The invention discloses a fish collagen composition as well as a preparation method and application thereof. The fish collagen composition comprises 45-65 parts of fish collagen peptide, 6-15 parts ofmaltodextrin, 10-15 parts of resistant dextrin, 5-10 parts of calcium aspartate, 1-5 parts of lemon powder, 0.1-1 part of acerola cherry powder, 5-15 parts of xylitol, 0.5-2 parts of xanthan gum, 1-2parts of anhydrous citric acid, 1-2 parts of lemon essence, and 0.01-0.2 part of sucralose. The fish collagen peptide is used as a main raw material, and the calcium aspartate is used as an auxiliarymaterial for complementing collagen and calcium, so that metabolism of skeleton collagen is promoted, chondrocyte is stimulated and actuated to strengthen synthesis of a cartilage matrix, bone jointrubbing is reduced, damaged articular cartilage is repaired, bone density is increased, and osteoporosis is prevented. The lemon powder and the acerola cherry powder contain massive rich vitamin C, sothat proliferation, formation and oxidation resistance of the collagen can be promoted; the lemon powder and the acerola cherry powder generate synergy effect with the fish collagen peptide and the calcium aspartate, so that the efficacy of products for beautifying the features is improved, and the effects of smoothing skin, enabling the skin to be elastic, preventing wrinkles and delaying senescence can be achieved.