25 results about "Biomimetic scaffold" patented technology

The invention relates to the technical field of bionic medical materials, in particular to a bionic scaffold material for promoting repair and reconstruction of infectious bone defects and a preparation method and application of the bionic scaffold material. The preparation method comprises the following steps: wrapping the oxygen vacancy titanium dioxide nanorod in collagen through temperature control, and directionally depositing mineralized crystals in a gap region in the collagen to obtain the porous bionic scaffold with a bone-like structure. The bionic scaffold material disclosed by the invention has a bone-like structure, not only can realize efficient physical antibiosis after ultrasonic irradiation, but also can adjust the intercellular effect of macrophages to promote tissue repair, and the repair and reconstruction effects of the material on infectious bone defects are verified in animal experiments. The successful preparation of the material can realize time-space controllable precise antibiosis, further promote the repair and regeneration of bone tissues, and provide a new method for clinical treatment of bone defects, especially bone defect related diseases accompanied by infection.

The application discloses a kind of multi-objective structure optimization design methods for porous biomimetic stent, the method includes the following steps: step one, by biology constraint, construct the constraint condition of stent structure parameter, and utilize genetic algorithm to obtain the feasible region satisfying constraint condition;Step two, based on feasible region, construct the three-dimensional model of porous biomimetic stent, carry out finite element analysis and fluid dynamics simulation calculation, to obtain the elastic modulus and permeability of stent structure;Step three, based on the mechanical properties and fluid properties of bone tissue, determine the target performance optimization design space consistent with it, to obtain the stent structure parameter design set meeting the performance requirements of bone tissue, determine the structure design scheme capable of simultaneously optimizing the mechanical properties and biological fluid properties of stent according to Pareto optimal solution.This method realizes the high decoupling of elastic modulus and permeability of stent under given porosity while considering multiple performance requirements.

The invention discloses a preparation method of a vascular organ chip based on a degradable bionic stent. The degradable biological scaffold (PCL-FBE scaffold for short) is obtained by mixing a swimming bladder extract and polycaprolactone and carrying out electrostatic spinning. On the basis, a vascular organ chip which can be integrated in a micro-fluidic system is further constructed, and a whole process for completing three-dimensional dynamic culture of vascular organs by using the chip is established. According to the present invention, the swim bladder extract and polycaprolactone are compounded to form the electrospinning scaffold, such that the defects of the mechanical property, the degradability and the biocompatibility of the traditional bionic scaffold are successfully overcome, the organoid culture effect is significantly improved, and the efficient and sustainable innovation platform is provided for the tissue engineering and the regenerative medicine.

The invention belongs to the technical field of tissue engineering, and particularly relates to a bio-ink system for osteochondral repair as well as a preparation method and application of the bio-ink system. The system comprises first bio-ink and second bio-ink which are respectively used for simulating cartilage and bone tissue. The first biological ink is prepared from methacrylic acid gelatin, acellular extracellular matrix powder, aldehyde modified natural polysaccharide, a photoinitiator and a transforming growth factor; the second biological ink is prepared from methacrylic acid gelatin, acellular extracellular matrix powder, nano-hydroxyapatite, aldehyde modified natural polysaccharide, a photoinitiator and human bone morphogenetic protein BMP-2. The bio-ink system can guarantee smooth extrusion and interlayer fusion in the printing process; the constructed bionic microenvironment and the affinity effect of the bionic microenvironment on growth factors are suitable for 3D printing construction of the osteochondral integrated bionic scaffold and have good clinical transformation potential.

The invention discloses a stem cell-loaded microcapsule, a preparation method and application, the stem cell-loaded microcapsule is composed of hydrogel and stem cells, and the stem cells are wrapped in the stem cell-loaded microcapsule; the stem cells are selected from at least one of umbilical cord mesenchymal stem cells, bone marrow mesenchymal stem cells, adipose-derived stem cells, embryonic stem cells and induced pluripotent stem cells; the number of living cells in the stem cell-loaded microcapsule is 1-10; the particle size of the stem cell-loaded microcapsule is 30-200m, and the dispersion coefficient of particle size distribution is 0.01-20%. According to the preparation method, stem cells and a biological material are mixed to prepare a prepolymerization solution, the biological material provides immune protection for the cells, the in-vivo retention time of the cells is prolonged, and the problem that at present, the stem cells are rapidly removed by an immune system after being transplanted into the body is solved. The capsule can be used as bio-ink to be assembled into a bionic stent through 3D printing or directly injected to an injured part, and has a wide application prospect.

The application discloses a kind of biomimetic composite scaffold with periosteum-cortical bone-cancellous bone structure and its preparation method and application.The scaffold is assembled by independent periosteum biomimetic scaffold module and independent bone trunk biomimetic module.The periosteum biomimetic scaffold module is cylindrical tube structure hydrogel, loaded with zinc silicate particles and periosteum related cells, with the biological functions of promoting cartilage differentiation, secreting nerve and vascular factors and synthesizing periosteum matrix proteins.The bone trunk biomimetic scaffold module is cylindrical bioceramic, with multiple vertical pipes embedded in the dense layer of outer wall to simulate cortical bone, and with staggered structure inside to simulate cancellous bone.Animal experiments show that the biomimetic composite scaffold can effectively promote the integrated regeneration of bone trunk and periosteum in rabbit radial critical size defect model.Therefore, the application provides a new solution for the biomimetic design and regeneration repair of bone tissue engineering.

The application relates to a 3D freeze printing system and printing method of an anisotropic support, the system comprising a refrigerant gas transmission device and a freeze printing auxiliary module; the freeze printing auxiliary module comprises a liquid nitrogen refrigeration ring, one or more gas flow channels with a set configuration and arrangement mode are arranged on the liquid nitrogen refrigeration ring, the air inlet of the gas flow channel is connected with the output end of the cold gas transmission device, and the air outlet of the gas flow channel is oriented to the printing platform of an extrusion type 3D printer in a specific direction, so as to guide the formation of an anisotropic microstructure with a specific orientation of a hydrogel support in a printing process. Compared with the prior art, the application can print a large-size anisotropic support with vertical and radial microstructures, strong mechanical properties, guided cell directional migration and endogenous tissue regeneration, and provides a brand-new biomimetic support solution for anisotropic tissue regeneration.

A rod-based porous biomimetic scaffold with dual adjustable parameters and its design method are disclosed. The method involves designing basic units based on Primitive units in a typical TPMS lattice; solving the volume fraction function expression based on the geometric relationships of the basic units; determining the volume fraction of the basic units for practical application according to the target scaffold and obtaining the dual adjustable parameters; establishing a three-dimensional model of the basic unit array arranged as a porous structure and 3D printing the rod-based porous biomimetic scaffold; and evaluating the mechanical properties and mass transfer performance of the rod-based porous biomimetic scaffold. The inclusion of two independently adjustable parameters—the diameters of the main and secondary load-bearing rods—enables a high degree of decoupling between its mechanical properties and mass transfer performance, giving the scaffold structure more possibilities.

The invention discloses a bone-periosteum bionic stent for vascularized bone regeneration and a preparation method of the bone-periosteum bionic stent, and belongs to the technical field of bone repair. The stent comprises a bone bionic stent and a bionic periosteum, the bone bionic scaffold is of a hierarchical pore structure and is formed by compounding polycaprolactone, silk fibroin and beta-tricalcium phosphate through fused deposition 3D printing, and the surface of the bone bionic scaffold is subjected to alkali treatment so as to improve hydrophilicity and osteogenic activity; the bionic periosteum is printed on the surface of the bone bionic scaffold through a near-field direct writing technology and is composed of polycaprolactone, mineralized collagen and magnesium ions, and the mineralized collagen is distributed in the bionic periosteum in a gradient mode. Through integrated bionic design, structures and functions of natural bones and periosteum are simulated; the bone bionic support plays a role in mechanical support and bone formation promotion; the bionic periosteum rapidly releases magnesium ions in the early stage of degradation, and early vascularization is promoted; along with degradation, continuously released collagen induces mineralization of a collagen matrix in the later period; the stent has good cell compatibility and capability of promoting osteogenic differentiation and blood vessel formation.

The invention discloses a bionic intervertebral disc integrated organoid based on 3D printing and a construction method and application thereof, and relates to the field of biomedical engineering. The construction method comprises the following steps that a polymer material is adopted to prepare a crossed net-shaped bionic stent through 3D printing, the crossed net-shaped bionic stent is curled to form a cylindrical fiber ring structure, and the fiber ring bionic stent is obtained; performing mesoderm induction on the pluripotent stem cells, and respectively performing cartilage induction and fibrous cartilage induction to obtain nucleus pulposus-like cartilage organs and fibrous ring-like fibrous cartilage organs; the cartilage-like organ is placed in the center of the fibrous ring bionic scaffold, the fibrous cartilage-like organ is filled in the grid structure of the fibrous ring bionic scaffold, and after matrigel wrapping and incubation curing, the bionic intervertebral disc integrated organ based on 3D printing is obtained through induction culture. The bionic intervertebral disc integrated organ based on 3D printing provided by the invention can effectively reproduce structural characteristics and functional characteristics of a natural intervertebral disc.

The application discloses a perspective image analysis method for bionic repair of bone tumors, comprising the following steps: acquiring perspective image data of a bone tumor area; performing cross-modal feature alignment on the data, constructing a three-dimensional bone tumor image model in a three-dimensional voxel space coordinate system, and eliminating image artifacts; performing segmentation processing on the bone tumor through a deep learning segmentation network, identifying a bone tumor infiltration boundary, and acquiring three-dimensional morphological parameters and bone tumor characteristic parameters of the bone tumor area; reconstructing a bionic scaffold structure of a bone defect area through biomechanical modeling, and implanting the bionic scaffold into the bone tumor area; and acquiring bone-scaffold interface fusion rate, stress distribution uniformity and new bone growth depth indexes based on postoperative image data. The application has the advantages that: through the combination of accurate segmentation at the voxel level and a convolutional neural network, the bone tumor area and morphology can be accurately identified and divided, thereby providing reliable support for subsequent tumor infiltration evaluation and bionic scaffold design.

The invention relates to the technical field of biotechnology and cell engineering, in particular to a large-scale culture method of central memory T cells (TCM). According to the method, the lymphocyte expressed by the high homing receptor is activated by adopting an alternating electric field, and the cell activity is improved by combining with a composite metabolism regulator. A two-photon excitation three-dimensional scaffold of a modified collagen peptide, ferroferric oxide and a quantum dot labeled organoid is utilized to simulate an in-vivo microenvironment to promote cell anchoring. And finally, through gene editing, engineering a cryopreservation system of saccharomyces cerevisiae and ultraviolet light activated autophagic peptide, so as to realize the enhancement of cell function stability and long-term memory characteristic. According to the invention, the limitation of traditional two-dimensional culture is broken through, and an efficient and controllable T cell preparation scheme is provided for tumor immunotherapy, autoimmune disease regulation and personalized cell therapy through systematic integration of physical field regulation, bionic scaffold design and genetic engineering means.

The present invention provides porous biomimetic scaffolds and methods for making the same. The scaffolds have graded pore sizes for enhanced cell penetration. The scaffolds are useful for wound regeneration by facilitating cell penetration into the scaffold interior and due to their inherent immunomodulatory effects. The scaffolds have tissue modeling specification by mimicking the inherent stratified structure of certain tissues.

The invention belongs to the technical field of biological products, and particularly relates to a bionic biological 3D printing active stent, a preparation method and application. The method comprises the following steps: respectively preparing decellularized matrix hydrogel (DSCM-G) from spinal cord tissues and decellularized matrix hydrogel (DNM-G) from peripheral nerve tissues, preparing a partitioned spinal cord bionic scaffold or a partitioned mold through 3D printing, then respectively pouring DSCM-G containing or not containing cells into a central region, pouring DNM-G containing or not containing cells into peripheral regions, and finally, preparing the partitioned spinal cord bionic scaffold or the partitioned mold through 3D printing. And gelatinizing and curing to obtain the multi-bionic spinal cord active stent. Bionic construction of grey matter and white matter is achieved through different biological materials and cells, spatial distribution construction of the grey matter area and the white matter area is achieved through the 3D printing technology, the components, modulus, micro-network structure and function of the material are closer to those of spinal cord tissue, good biocompatibility is achieved, and the material can be applied to the field of spinal cord tissue engineering. And synchronous collaborative recovery of grey matter and white matter after spinal cord injury is more likely to be realized.

The application provides a dental implant bionic support, which comprises a main support, a fixing wing arranged on the side of the main support, a fixing hole is arranged in the fixing wing, an absorbable fixing nail is arranged in the fixing hole to position the main support on the alveolar bone, the main support is arranged between the alveolar bone and the implant, and an array of grooves is arranged on the side of the main support close to the implant, the grooves are used for guiding and restraining fibroblasts; the application can greatly simplify the preparation difficulty of the autologous root piece, and prevent the problems of complications caused by the breakage and perforation of the autologous root piece.

The invention discloses a polydatin tendon-bone repair gradient bionic scaffold and a preparation method thereof, a nano-hydroxyapatite (nHAP) / polycaprolactone (PCL) composite material and a biological hydrogel containing polydatin (PLD) liposome are designed and prepared by 3D printing to form a multi-partition structure, so as to meet the complex requirements of tendon-bone healing. Wherein the nHAP / PCL composite material has good mechanical performance and is matched with a tendon stress environment to serve as a mechanical framework of the stent. The introduction of nHAP can effectively promote the healing of a bone injury area, and the construction of the slow-release PLD liposome hydrogel can effectively reverse the fibrosis of a tendon-bone interface. The gradient bionic scaffold for slowly releasing the polydatin, which is constructed by a 3D printing technology, can bring a brand-new treatment scheme for tendon-bone healing.

The present application relates to the technical field of biomimetic medical materials, in particular to a kind of biomimetic scaffold material for promoting the repair and reconstruction of infected bone defect and its preparation method and application.The preparation method is, by temperature control, oxygen vacancy titanium dioxide nanorod is wrapped in collagen inside, and makes mineralized crystal directional deposition in collagen internal gap area, obtains the porous biomimetic scaffold with bone-like structure.The biomimetic scaffold material of the present application has bone-like structure, not only can realize efficient physical antibiosis after ultrasonic irradiation, but also can adjust the cytophagous action of macrophage to promote tissue repair, and the repair and reconstruction effect of the material for infected bone defect has been verified in animal experiment.The successful preparation of the present application material can realize the precise antibiosis of space-time controllable, and further promote the repair and regeneration of bone tissue, provide a new method for the clinical treatment of bone defect, especially the bone defect related diseases with infection.

The invention relates to the technical field of preparation of medical stem cell preparations, and discloses a preparation method and a preparation system of a stem cell preparation for repairing joint injury, according to the method, autologous bone marrow MSCs are sorted through microfluidic, SOX9 genes are activated through CRISPR-Cas9 dual targeting, HLA-I / I is knocked out, then a bionic scaffold is subjected to 3D printing based on MRI data, and the stem cell preparation for repairing joint injury is obtained. The method comprises the following steps: fixing stem cells on a stent in a cross-linking manner, loading an exosome through CD63-amino covalent coupling, applying 0.5-1Hz periodic compression load culture according to a viscoelastic model, and performing gradient cryopreservation in a trehalose protective agent; the system comprises five modules: a stem cell pretreatment module, a bionic scaffold construction module, a cell-scaffold compounding module, a dynamic culture module and a cryopreservation quality inspection module. According to the method, the cartilage differentiation efficiency is improved by more than 3 times by utilizing gene editing, the exosome slow release period is prolonged to 14 days, meanwhile, the secretion amount of collagen I is improved by 89% through dynamic culture, and the freezing survival rate is greater than 96%; and the quality inspection qualification rate is 100%.

The application discloses a kind of multilayer biomimetic scaffolds and preparation method thereof.The preparation method of the multilayer biomimetic scaffold includes the following steps:S1, sacrificial material is layer-by-layer 3D printed, n layers are continuously printed, and grid-shaped porous inner mold is obtained;S2, biomimetic hydrogel precursor solution is poured in the grid-shaped porous template, then light curing crosslinking is carried out, and sacrificial material-hydrogel complex is formed;S3, repeat step S1 and S2, and form multilayer sacrificial material-hydrogel complex;S4, solvent dissolution method is used to carry out inner demolding treatment to the multilayer sacrificial material-hydrogel complex, and multilayer biomimetic scaffold is obtained.The preparation method realizes layered biomimetic design based on inner demolding technology, ensures that hydrogel of different biomimetic layers exists independently, avoids mixing and diffusion problem of biomimetic material between adjacent layers when pouring once, accurately realizes the design of different biomimetic layers, and also ensures effective curing.

The invention discloses an integrated printing method for a critical bone scaffold of a controllable disordered bionic structure, which comprises the following steps of: (1) constructing a bionic scaffold with controllable disordered arrangement by utilizing a melt near-field direct writing technology so as to simulate the tissue characteristics of cancellous bone; (2) establishing a dynamic control system of voltage and air pressure, wherein the on-off and numerical value of the high-voltage electric control system are directly controlled by a PLC according to the duration required by each printing unit; according to the air pressure control, a PLC drives a high-precision proportional valve to dynamically adjust air pressure output, and an air pressure value is monitored and displayed in combination with PID, so that the accuracy of air pressure output is ensured; and (3) on the basis, constructing an external cortical bone structure of the stent by adopting a fusion thermal deposition technology, and cooperating with a cancellous bone structure printed by melt near-field direct writing to realize multi-scale design and complete functional partition and integrated construction of the cancellous bone and the cortical bone. The method not only effectively simulates the characteristics of natural bone tissues, but also remarkably improves the mechanical properties of the whole stent.

The application discloses a lacrimal passage epithelial organoid and a construction method and application thereof, and the construction method comprises the following steps: S1, preparation of a three-dimensional biomimetic support; S2, acquisition of lacrimal passage epithelial stem cells; and S3, construction and culture of the organoid. The PCL / GEL nanofiber support constructed by the electrospinning technology is combined with Matrigel coating, the extracellular matrix (ECM) microenvironment of the in-vivo lacrimal passage mucosa is successfully simulated, and the technical difficulties that stem cells are prone to aging and differentiation direction disorder in traditional two-dimensional culture are fundamentally solved. This breakthrough result promotes the lacrimal passage tissue engineering from simple 'cell adhesion growth' to a new height of'structure-function synchronous reconstruction', and provides a double technical guarantee of seed cells and tissue engineering supports for solving the clinical core pain point of postoperative scar adhesion and re-occlusion of the lacrimal passage obstruction disease.

This invention provides a composite hydrogel biomimetic scaffold and its preparation method. The method includes preparing a phenylboronic acid-modified chitosan and hyaluronic acid solution; preparing carboxylated polyvinyl alcohol; preparing the two into a polyvinyl alcohol-chitosan-hyaluronic acid hydrogel; adding an anti-ferroptosis drug and an anti-P2X4 receptor drug to the polyvinyl alcohol-chitosan-hyaluronic acid hydrogel to obtain a drug-loaded composite hydrogel; preparing a hybrid material with a bio-glass sheet; and fabricating the hybrid material into a biomimetic scaffold using 3D printing. Through dynamic borate ester bonds as crosslinking and responsive units, the biomimetic scaffold can achieve rapid release of the loaded drug. By adding an anti-ferroptosis drug and an anti-P2X4 receptor drug, it synergistically addresses the intertwined pathological states of oxidative stress, ferroptosis, and neuropathic pain after nerve injury through three key pathways: inhibiting lipid peroxidation, chelating free iron, and blocking purine receptor-mediated neuroinflammation.

The invention discloses a multilayer bionic stent and a preparation method thereof. The preparation method of the multilayer bionic scaffold comprises the following steps: S1, performing layer-by-layer 3D printing on a sacrificial material, and continuously printing n layers to obtain a latticed porous inner mold; s2, a bionic hydrogel precursor solution is poured into the latticed porous template, then light curing crosslinking is carried out, and a sacrificial material-hydrogel complex is formed; s3, repeating the steps S1 and S2 to form a multi-layer sacrificial material-hydrogel complex; and S4, performing internal demolding treatment on the multi-layer sacrificial material-hydrogel complex by adopting a solvent dissolution method to obtain the multi-layer bionic stent. According to the preparation method, layered bionic design is achieved based on an internal demolding technology, it is ensured that hydrogel of different bionic layers exists independently, the problems of mixing and diffusion of bionic materials between adjacent layers during one-time perfusion are solved, design of the different bionic layers is accurately achieved, and meanwhile effective curing is also ensured.

This invention discloses a method for parametric design of biomimetic scaffolds based on three-period minimal curved surfaces, relating to the field of biomimetic bone scaffold technology. The specific method involves selecting Gyroid(G) units in the TPMS structure within a three-period minimal curved surface structure. A bias function is designed to cause the porosity of the novel porous structure to increase incrementally along the radial direction from both the edge and center. Subsequently, the novel porous structure is imported into mechanical simulation software for mechanical performance testing, and the compression deformation behavior of each model is observed. This invention improves upon the use of general uniform porous structures as biomimetic bone scaffolds, considering the actual stress conditions of the femur during daily human activities, including not only compressive loads but also shear forces, and is applicable to all types of TPMS structures.