40 results about "Biomimetic scaffold" patented technology

The present invention relates to a layered chitosan scaffold wherein said layered scaffold comprises at least two fused layers, wherein at least one of the fused layers comprises a chitosan nanofiber membrane and the other fused layer comprises a porous chitosan support layer. Moreover, the present invention provides a layered chitosan scaffold characterized by (i) a good adhesion between the porous and nanofiber layers, (ii) a tuneable porosity of the nanofiber layer by tuning the distance between the nanofibers, (iii) a stable nanofibers and porous morphology even when immersed in water or other solvents and a process for the preparation of such layered chitosan scaffold. The present invention also provides a process for the preparation of the layered chitosan scaffold.

The invention discloses a biomimetic hydroxylapatite powder/gelatin/sodium alginate composite 3D print bracket and a preparation method thereof. The preparation method of the biomimetic 3D bracket comprises the steps of (1) preparing hydroxylapatite powder having a biomimetic classified structure; (2) preparing hydroxylapatite powder/gelatin/sodium alginate composite pulp; and (3) preparing the biomimetic 3D print bracket. According to the biomimetic hydroxylapatite powder/gelatin/sodium alginate composite 3D print bracket and the preparation method thereof disclosed by the invention, a 3D printing technique is utilized, the prepared organic/inorganic composite three-dimensional print bracket is controllable in outside size and good in biocompatibility, components and a multi-level structure of natural bone tissue are sufficiently simulated, and controlled mechanical strength is given to materials; and the micro-nano multi-level structure of the composite bracket has higher osteogenesis activity and osteoinductive, a multi-level pore structure can effectively induce ingrowth of tissue and blood vessels, and regeneration and repair of the bone tissue are promoted.

The invention discloses a preparation method and application of a silk fibroin and chitin blended nanofiber embedded hydrogel cartilage biomimetic scaffold. The method utilizes mulberry silk to prepare regenerated the silk fibroin, purifies industrial grade chitin, mixes a silk fibroin solution and a chitin solution, prepares a blended nanofiber membrane by an electrospinning technique, cross-links with ethanol to form a short fiber through a blended nanofiber, mixes the short fiber and the silk fibroin solution, conducts cross-linking by a crosslinking agent, and conducts freeze-forming to obtain a spongy cartilage bionic scaffold. In particular, the insertion of the nano short fiber increases the compressive strength and biocompatibility of the scaffold material. The method constructs the biomimetic scaffold with good biocompatibility, low immune rejection and similar composition with natural cartilage matrix, has wide material source, low cost and simple preparation process, can beused for constructing tissue engineering cartilage and repairing cartilage regression, and has good clinical application prospects.

The invention provides a drug/factor control-released thin film scaffold. The drug/factor control-released thin film scaffold is characterized by comprising a film-like bionic scaffold, a PDGF (Platelet Derived Growth Factor) coating layer and a slow release layer which are sequentially arranged. According to a scaffold material, i.e, a film-like scaffold material on the surface layer of a factor/the core layer of a drug, for clinic periodontal treatment, provided by the invention, controlled release of active factors and drugs is realized, and cell proliferation and osteogenic differentiation are promoted, so that three elements of seed cells, a scaffold material and regulatory factors in tissue regeneration are organically combined, periodontal repairing is promoted, and a facilitation function for periodontal tissue engineering research is obtained.

A novel method for building complex three-dimensional scaffolds for biomimetic applications such as in vitro organ growth, using a gelatin/sugar/water gel, ultraviolet radiation, and heat or enzyme is described. The method produces gelatin-sugar hydrogels demonstrating greater thermal stability, mechanical strength, and resistance to enzymatic degradation. The invention also provides a means to assemble the gelatin sugar hydrogel films into complex three-dimensional structures (scaffolds). To account for the native biochemical factors present in natural scaffolds, methods of conjugating such factors to the gelatin-sugar hydrogel are described. These scaffolds can then be applied for tissue culturing and organ growth. The present invention also describes a system and apparatus for constructing these complex three-dimensional scaffolds by taking advantage of the physical and chemical properties of the gelatin-sugar hydrogel.

The invention discloses a preparation method of a super-bionic soft and hard tissue composite scaffold. The preparation method comprises the following steps: designing a bionic bone tissue scaffold, preparing a printing raw material, preparing printing ink, performing DLP photocuring 3D printing, performing freeze drying, extracting dental pulp mesenchymal stem cells of a patient, performing directional differentiation, inoculating and culturing the cells, preparing a soft tissue scaffold and preparing a soft and hard tissue composite scaffold. Through 3D printing of the super-bionic biological material, simultaneous personalized repair of oral clinical soft and hard tissues is achieved, development of a second operation area is avoided, and the treatment time for stage repair of the soft and hard tissues at present is shortened. Meanwhile, the three-dimensional microstructure of the jawbone tissue and the Harvard tube and the Walkman tube simulating the jawbone are inspired to establish good blood supply in the early stage, conditions are provided for layered induction of the bionic jawbone tissue, and a new thought is provided for design, research and development of the bionic scaffold.

The invention relates generally to generation of biomimetic scaffolds for bone tissue engineering and, more particularly, to multi-level lamellar structures having rotated or alternated plywood designs to mimic natural bone tissue. The invention also includes methods of preparing and applying the scaffolds to treat bone tissue defects. The biomimetic scaffold includes a lamellar structure having multiple lamellae and each lamella has a plurality of layers stacked parallel to one another. The lamellae and/or the plurality of layers is rotated at varying angles based on the design parameters from specific tissue structural imaging data of natural bone tissue, to achieve an overall trend in orientation to mimic the rotated lamellar plywood structure of the naturally occurring bone tissue.

The invention provides an aperture gradient porous scaffold and a minimal curved surface structure for the same. The minimal curved surface structure unit is a Gyroid curved surface structure unit, aPrimitive curved surface structure unit, a Diamond curved surface structure unit or an I-WP curved surface structure unit, and the Gyroid curved surface structure unit, the Primitive curved surface structure unit, the Diamond curved surface structure unit and the I-WP curved surface structure unit are respectively controlled by implicit function expressions. The pore diameters of the Gyroid, Primitive, Diamond and I-WP curved surface structure units are 200-1000 [mu]m, the porosity of the Gyroid, Primitive, Diamond and I-WP curved surface structure units is 10-90%, and the lengths a, b and c of the Gyroid, Primitive, Diamond and I-WP curved surface structure units in the x, y and z directions are 0.5-2 mm. The bionic scaffold with the pore structure and function similar to those of the natural bone is obtained based on the minimal curved surface structure unit.

The invention relates to a novel bionic scaffold model constructed through a 3D-Voronoi algorithm and a random distribution micropore algorithm, and a high-precision tissue engineering scaffold is manufactured by using a biological 3D printing technology. The bovine femur is calcined by using an ammonium dihydrogen phosphate secondary calcination method, inorganic components such as hydroxyapatiteand the like are removed on the basis of realizing allogeneic bone degreasing, decalcification and antigen removal, so that the remainder is high-purity beta-tricalcium phosphate, and variable difference caused by a matrix material in an experiment is eliminated while the form of a natural cancellous bone is retained. Through various experiments such as characterization detection, structural analysis, in-vitro experiments and in-vivo implantation, the influence of different scaffold structures on cell proliferation and adhesion and in-vivo bone repair effects is comprehensively evaluated, a reliable scaffold model and an effective detection means are provided for bionic structure design of the bone tissue engineering scaffold, and the bone tissue engineering scaffold can be used for clinically treating various bone defects.

The invention discloses an active extracellular matrix, a composition containing the active extracellular matrix, a 3D tissue repair scaffold, and a preparation method and application. The method comprises the following steps of introducing a gene segment for expressing an active factor into a cell in advance, and carrying out in-vitro cell culture to obtain the extracellular matrix containing theactive factor, so that the reliability of cell origin is ensured while imparting stability and modifiability or processability to the quality of extracellular matrix products. According to the method, stem cell culture and directional differentiation are carried out on a 3D printed porous scaffold to prepare a bionic scaffold capable of promoting damage repair, a material design principle and a stem cell differentiation technology based on stem cell combined directional differentiation are established, and a new field of stem cell and material combination in in-situ tissue engineering repairapplication is developed. According to the method, the bionic scaffold is further optimized, induced adsorption, growth and differentiation of cells after implantation are achieved, and tissue (bone,cartilage, skin, myocardium and blood vessels) regeneration is achieved.

The invention discloses a bionic eyelid scaffold having a double-layer composite structure and a construction method thereof. The bionic eyelid scaffold is mainly formed by compounding of two layers of different bionic eyelid tissue materials; a bottom layer is a porous degradable elastic polyester skeleton, and a surface layer is a natural biological material, that is a sponge; and porous degradable elastic polyester is taken as a raw material, an eyelid skeleton material having a communicating pore is prepared by using a gelatin template method, and the natural biological material is composited to the eyelid skeleton material, thus obtaining the bionic eyelid scaffold having the double-layer composite structure in tissue engineering. The elastic polyester/natural biological material composite scaffold prepared by the construction method of the invention has a matched mechanical strength and maintains an eyelid form, and simultaneously has a good biocompatibility and can promote growth and functional maintenance of fibrocytes and conjunctival cells; and the preparation method is simple, and the repeatability is good.

The present invention relates to biomimetic scaffolds, methods for making the same, and methods for using the same. The scaffolds comprise a plurality of graded or tapered microchannels that provide spatial control for cell penetration. The scaffolds are useful for regenerating missing interface tissue between two adjacent tissues, or regeneration and integration of two adjacent tissues directly.

The invention discloses a spherical nano hydroxyapatite/natural polymer bionic scaffold and a preparation method thereof. The problems are solved that in the prior art, the particle morphology, size and content of hydroxyapatite are uncontrollable and the distribution is uniform. The preparation method of the spherical nano hydroxyapatite/natural polymer bionic scaffold includes the following steps that a natural polymer is dissolved into deionized water to form a polymer solution; the polymer solution is cross-linked to form polymer gel; a soluble calcium salt solution is dropwise added on the surface of the gel for diffusion; then a soluble phosphorus-containing solution is dropwise added onto the surface of the gel for further diffusion; after diffusion, the solution left on the gel isremoved, the gel is soaked in an alkaline solution, then the gel is washed in sequence with a phosphate buffer solution (PBS) and deionized water, and after freeze drying, the three-dimensional porousbionic scaffold is obtained. The design is scientific, the method is simple and operation is simple and convenient.

Disclosed herein are biomimetic scaffolds for nervous tissue growth having a plurality of microchannels disposed within a sheath. Each microchannel includes a porous wall formed of a biocompatible and biodegradable material. The biocompatible and biodegradable material may be a poly (ethylene glycol) diacrylate, a methacrylated gelatin, a methacrylated collagen, or a polycaprolactone, and combinations thereof. The bionic stent has a high open volume percentage, can achieve excellent (linear and high fidelity) nervous tissue growth, and meanwhile reduces inflammation near an in-vivo implantation site to the maximum extent.

The invention relates to a method for constructing in-vitro tissue engineering cartilage, and the method comprises the following steps: inoculating cartilage cells onto a cartilage bionic scaffold, and applying stepped dynamic hydrostatic pressure to stimulate culture after adhesion, wherein the average pore size of the cartilage bionic scaffold is more than 100 microns, the stepped dynamic hydrostatic pressure means that the hydrostatic pressure changes in a stepped mode along with time, the number of steps is two or more, the hydrostatic pressure corresponding to each step is selected within the range of 0.1 MPa to 10 MPa, the duration time of the hydrostatic pressure corresponding to each step is selected within the range of 10 s to 100 s, and the difference between the hydrostatic pressures corresponding to the adjacent steps is 1.5 times or above. The in-vitro tissue engineering cartilage with good cartilage phenotype maintenance is constructed by combining construction of a proper natural protein cartilage bionic scaffold and stepped circulating dynamic hydrostatic pressure stimulation culture, and the mechanical property, porosity and other characteristics of the scaffold material are all suitable for adhesion growth and phenotype maintenance of cartilage cells; the stepped circulating dynamic hydrostatic pressure stimulation shows excellent cartilage cell growth promotion and phenotype maintenance capabilities.

The invention relates generally to generation of biomimetic scaffolds for bone tissue engineering and, more particularly, to multi-level lamellar structures having rotated or alternated plywood designs to mimic natural bone tissue. The invention also includes methods of preparing and applying the scaffolds to treat bone tissue defects. The biomimetic scaffold includes a lamellar structure having multiple lamellae and each lamella has a plurality of layers stacked parallel to one another. The lamellae and/or the plurality of layers is rotated at varying angles based on the design parameters from specific tissue structural imaging data of natural bone tissue, to achieve an overall trend in orientation to mimic the rotated lamellar plywood structure of the naturally occurring bone tissue.

The invention discloses a flying toy device based on infrared induction technology, which is composed of a bionic bracket, a power device and a remote control device. Composed of power components; the bionic bracket includes a main frame, a head frame, a wing frame and a tail frame. The present invention adopts remote control and is equipped with a power system, which drives the flapping mechanism to move through the gear set, thereby converting it into flying power, so that Toys are more vivid, realistic and intelligent.