168 results about "Bones regeneration" patented technology

The invention discloses a special-shaped titanium alloy capable of enhancing bone regeneration and repair efficacy as well as a preparation method and application of the special-shaped titanium alloy. The special-shaped titanium alloy comprises a special-shaped titanium alloy base body and a porous structure attached to the special-shaped titanium alloy base body, and the porous structure is in multi-stage size distribution and comprises long-strip-shaped gullies (100-150 micrometers wide) and large holes (30-50 micrometers wide). And the morphology in the gullies shows coralline ligaments and a nest-shaped pore channel (0.5-2 microns) structure. This can provide space and sites for the growth and adsorption of osteoblasts. According to the method, a gas phase alloying (VPA) and VPD synergistic process is adopted in a breakthrough mode, a non-oxidation / nitridation coralline-shaped hierarchical pore structure is constructed on the surface of the commercial titanium implant, and it can be achieved through verification of an aging animal model that a special-shaped matrix is uniformly modified in the whole domain, the regeneration function of aging stem cells is activated, and the bone repair and regeneration effect is effectively improved.

The invention provides a composition for changing plasticity of bone meal particles as well as a preparation method and application of the composition, and relates to the technical field of biomedical materials. The composition is prepared from the following raw material components: bioactive glass, dihydrazide modified sodium hyaluronate, sodium hyaluronate with the molecular weight of 50kDa to 500kDa, a polylactic acid-glycolic acid copolymer and carboxymethyl cellulose. The preparation method of the dihydrazide modified sodium hyaluronate comprises the following steps: carrying out carboxyl activation on sodium hyaluronate, carrying out a cross-linking reaction on the activated hyaluronic acid and adipic dihydrazide, and then terminating the reaction. The composition provided by the invention not only can provide support, but also can adjust the degradation rate to be matched with the bone regeneration period, and all the components synergistically improve the mechanical property and the viscoelastic property. The composition disclosed by the invention can be formed in situ at a bone defect part, provides a space for growth and proliferation of bone cells, improves the biological activity and osteoinductivity of an artificial bone, and promotes bone growth and repair.

The invention provides a composite hydrogel for promoting diabetic bone regeneration, the composite hydrogel is composed of L-histidine modified chitosan, oxidized konjac glucomannan, Zn and nano clay, and in-situ gelation is realized through coordination regulation, interlayer exchange and dynamic covalent bond synergistic effect. Through combined application of coordination regulation, interlayer exchange and dynamic covalent bonds, on-demand staged delivery of various ions is realized. According to the innovative design, the adhesive property and mechanical integrity of the hydrogel at a defect part are maintained, intelligent response can be realized according to the change of a bone microenvironment, and the defect that a single release mechanism in the prior art is difficult to meet the complicated demand of diabetic bone regeneration is overcome.

This invention relates to the field of biomedical technology, specifically to a biomimetic piezoelectric scaffold for promoting bone repair, its preparation method, and its applications. The biomimetic piezoelectric scaffold provided by this invention is produced by electrospinning PLLA into oriented nanofibers, and then modifying the fiber surface with nano-hydroxyapatite. To enhance the adhesion of hydroxyapatite to the fiber surface, a polydopamine layer is modified between the two as a connecting layer, ultimately resulting in a biomimetic piezoelectric scaffold with high porosity, good osteointegration, and osteogenic promoting effects. This scaffold exhibits good bone regeneration promoting effects in in vitro MSC recruitment, osteogenic differentiation experiments, and repair of skull bone defects in rats.

The invention discloses a bionic Voronoi-nickel titanium-hydrogel heterostructure implant and a preparation method thereof, and relates to the technical field of bone implantation, the construction of a Voronoi porous structure and the preparation of a bionic Voronoi-nickel titanium skeleton are completed through an innovative design method by referring to the structural concept of human bone trabecula, and then the bionic Voronoi-nickel titanium skeleton is used as a substrate to prepare the bionic Voronoi-nickel titanium hydrogel heterostructure implant. And preparing the bionic Voronoi-nickel titanium-hydrogel heterostructure implant by adopting a drying gel forming method. The implant integrates the mechanical advantages of high strength and low modulus of the nickel-titanium alloy and the structural characteristics of high connectivity and high specific surface area of the bionic Voronoi porous structure, the defect that hydrogel is weak in bearing is effectively overcome, meanwhile, the hydrogel has the buffering and energy absorbing effects, multifunctional time sequence delivery and release of antibiosis, anti-inflammation and bone regeneration promotion can be achieved, and the requirements of all stages of bone repair are met.

This invention discloses recombinant umbilical cord mesenchymal stem cells related to pentamericin 3 in the field of genetic engineering, their preparation method, and applications. The technical problem this invention aims to solve is how to provide a cell product capable of treating bone regeneration insufficiency. To solve this technical problem, this invention provides recombinant umbilical cord mesenchymal stem cells, which are prepared according to a method comprising the following steps: upregulating or enhancing or increasing the content and / or activity of pentamericin 3 or the expression of the gene encoding said protein in the umbilical cord mesenchymal stem cells, thereby obtaining the recombinant umbilical cord mesenchymal stem cells. The recombinant umbilical cord mesenchymal stem cells provided by this invention can be used to treat diseases related to bone regeneration insufficiency.

The invention discloses a gradient degradation type personalized absorbable sternum fracture fixing plate and a preparation process, and belongs to the technical field of medical implant materials. Comprising an anatomical adaptive plate body and an integrated locking assembly, the plate body is of a partitioned structure of a middle supporting area and an edge buffering area, a gradient pore array penetrating through the thickness direction is formed in the surface of the plate body, and a hydroxyapatite-collagen composite coating is loaded on the inner wall of a pore; the plate body is made of a composite absorbable material through 3D printing forming and hot pressing post-treatment, and the composite absorbable material comprises, by weight, 60%-70% of polylactic acid-glycolic acid copolymer (PLGA, LA / GA = 75 / 25), 15%-20% of silanized magnesium alloy microwires, 5%-10% of hydroxyapatite nano-particles and 5%-10% of polycaprolactone (PCL). Space-time matching of the degradation rate and the bone healing process is achieved through the partition gradient structure, the magnesium alloy microwires enhance the mechanical property and release active ions to promote osteogenesis, and the method has the advantages of personalized adaptation, gradient degradation, bone regeneration promotion and the like.

The invention provides a multi-nozzle 3D printing composite scaffold capable of promoting bone regeneration as well as a preparation method and application thereof, the multi-nozzle 3D printing composite scaffold is prepared by adopting multi-nozzle collaborative printing of a 3D printing system, the multi-nozzle 3D printing composite scaffold comprises a synthetic polymer material printed by a high-temperature nozzle and a hydrogel material printed by a low-temperature nozzle, and microfilament patterns formed by the two materials are alternately arranged. Wherein the synthetic polymer material is prepared from polycaprolactone and magnesium oxide nano particles; the hydrogel material is prepared from methacrylated gelatin, methacrylated gilan gum and double drug loading nano particles. The 3D printing composite scaffold provided by the invention has excellent mechanical properties, the double-drug-loaded nanoparticle sequence released osteogenesis promoting and vascular promoting drug coupling osteogenesis-vascularization promoting effects, released magnesium ions synergistically enhance osteogenesis and vascularization performance, and the 3D printing composite scaffold has a good bone defect repair effect and has application potential in the field of bone tissue repair.

The utility model provides a fibrous membrane for guiding periodontal tissue regeneration, and relates to the technical field of medical instruments. Preparing a PLGA double-layer fiber membrane loaded with drug-loaded sodium hyaluronate particles through the cooperative work of electrostatic spinning and electrostatic spraying; the sodium hyaluronate particles can greatly improve the loading rate of hydrophilic anti-inflammatory antibacterial and bone regeneration promoting medicines; a PLGA compact layer in the PLGA double-layer fiber membrane is loaded with first sodium hyaluronate particles loaded with anti-inflammatory and antibacterial drugs, and when the first sodium hyaluronate particles make contact with gingival tissue, cell adhesion and permeation can be hindered, and the anti-inflammatory and antibacterial effects can be achieved; a PLGA loose layer in the PLGA double-layer fiber membrane carries second sodium hyaluronate particles loaded with a bone regeneration promoting medicine, and the second sodium hyaluronate particles can promote osteoblast adhesion and facilitate dentale regeneration when making contact with bone tissue.

The utility model relates to periodontal surgery technical field, especially a kind of periodontal surgery granulation tissue removal instrument set, including, placing tray, the placing tray is provided with the adaptation area that can place six appliances, the placing tray includes the square open-bottom portion with waist line type, and the limiting plate is set in its inner cavity two sides, the rotating handle is movably provided in the length direction both ends of placing tray;Six The appliance is bone defect granulation removal instrument and alveolar crypt granulation removal instrument respectively.The application scheme can effectively help doctor to realize the removal of granulation tissue in clinical process, reduce the influence of residual granulation tissue on periodontal tissue and bone regeneration, reduce the muscle fatigue of doctor, improve the debridement efficiency and regeneration success probability.

The invention relates to a metal ion-doped osteogenesis nano enzyme and application thereof, and belongs to the technical field of biological medicines. The metal ion-doped osteogenesis nano-enzyme is prepared by partially replacing iron ions in crystal lattices of the Prussian blue nano-enzyme with active metal ions by utilizing the open type crystal structure characteristic of the Prussian blue nano-enzyme. On the premise of keeping the original catalytic performance of the Prussian blue nano enzyme, the osteogenic nano enzyme can endow a material with a new biological activity function, so that the diabetic bone microenvironment is accurately regulated, and the bone tissue regeneration in a hyperglycemia environment is powerfully promoted. The osteogenic nano-enzyme is integrated into a PCL (polycaprolactone) scaffold, so that the scaffold can be endowed with strong osteoinduction, anti-oxidation and anti-inflammatory functions, and a good foundation is laid for application of the osteogenic nano-enzyme in diabetic bone regeneration.

The present invention relates to a two- or three-layer film composition for periodontal use, comprising a hydrophobic polymer outer layer and one or two inner layers in the form of electrospun micro / nanofibers based on marine sulfated polysaccharides and calcium.

To provide a novel Lactobacillus paracasei strain with bone regeneration effects, and pharmaceutical and food compositions containing the same. [Solution] The present invention relates to a novel Lactobacillus paracasei L-30 and its uses, and more particularly to Lactobacillus paracasei L-30 (KCTC16035BP) which has the ability to promote the differentiation or formation of osteoblasts, the ability to suppress the differentiation or formation of osteoclasts, and the ability to promote immune activity, and which can regenerate bone, and to a pharmaceutical composition for the treatment or prevention of bone diseases containing the same.

The invention discloses a shapeable bone repair material capable of being implanted in a positioning manner and a preparation method thereof. The interior of the bone repair material is a core layer made of a gelatin, gallic acid and hydroxyapatite particle composite material, and the exterior of the bone repair material is a porous spongy collagen wrapping layer; the density of the wrapping layer is 20-100mg / cm < 3 >; the density of the core layer is 100-800 mg / cm < 3 >; and the particle size of the hydroxyapatite particles is 0.1-2 mm. The collagen sponge on the outer layer of the material wraps the gelatin / hydroxyapatite composite material, so that the problems of loose structure, weak binding force and displacement and powder falling after implantation of bone meal or collagen bone meal composite materials are solved, stimulation and trauma of bone meal particles to surrounding tissues can be avoided, and the conditions of bone meal discharge, bone grafting part collapse and the like are avoided. And in the self tissue regeneration process, the collagen component is degraded and absorbed, and the hydroxyapatite is fused with the new bone, so that bone regeneration is realized, and the success rate of tooth implantation can be improved.

The application relates to a guided bone regeneration composite barrier membrane based on inverse chirality topological deformation and temperature-sensitive shape memory and a preparation method thereof, and belongs to the technical field of medical devices. The barrier membrane comprises a metal framework and a base film layer completely wrapping the metal framework, wherein the metal framework comprises a plurality of rows of first circular rings and second circular rings in staggered distribution, and the first circular ring and the adjacent second circular ring are connected with a connecting rod in tangential connection. The application realizes the synergistic treatment effects of minimally invasive implantation, in-situ self-expansion, nail-free soft anchoring and full degradation through a micro-nano immersion process and reverse thermodynamic programming.

1. Name of the product in this design: Barrier Membrane for Guided Bone Regeneration (I). 2. Purpose of this design: To guide the use of a barrier membrane in bone regeneration procedures. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: the front view. 5. This design is a two-dimensional product, so the left view is omitted; this design is a two-dimensional product, so the right view is omitted; this design is a two-dimensional product, so the top view is omitted; this design is a two-dimensional product, so the bottom view is omitted.

The invention provides a preparation method and application of a BMP-2 / Apt19S two-factor immobilized mesoporous bioglass material, and belongs to the technical field of biomaterials. The mesoporous bioglass material with an ordered mesoporous structure, high specific surface area and good biological activity is stably prepared by combining a sol-gel method with a surfactant template technology; by introducing carboxyethyl silanetriol sodium, carboxyl functionalization on the surface of the material is realized, and further through amide reaction, a deoxynucleotide fragment Apt19S is grafted, so that the material has good stem cell collection capability and bone-induced growth factor BMP-2 loading capability; the method is simple in process, and the biological activity of the growth factor can be well maintained. The prepared BMP-2 / Apt19S two-factor immobilized mesoporous bioglass material is expected to realize targeted enrichment and slow release of drugs in the field of bone defect repair, improves bone regeneration efficiency, and has important clinical application prospects.

This invention discloses a method for preparing biomimetic calcium phosphate particles carrying type III recombinant human collagen, relating to the field of new biomedical materials. The particle formulation, by mass percentage, contains 50%-90% hydroxyapatite and 10%-50% type III recombinant human collagen. The preparation process involves artificially synthesizing hydroxyapatite precipitate, then compounding it with type III recombinant human collagen. The compounded material is then spread, naturally dried, crushed, and sieved to form particles. The resulting particles have a uniform and controllable particle size. When mixed with blood or water, they can quickly form a high-viscosity, malleable, and stable filling material, directly adapting to the site preservation requirements of alveolar sockets after tooth extraction. The advantages are: the degradation rate of these particles precisely matches the bone regeneration rate after site preservation surgery, efficiently guiding bone tissue regeneration while significantly shortening the waiting period for implantation after tooth extraction. This solves the clinical promotion difficulties caused by the long waiting period and complex operation of site preservation surgery, contributing to the widespread application of this technology in oral clinical practice.

The application discloses a kind of biomimetic Voronoi-nickel titanium-hydrogel heterostructure implants and preparation method thereof, it is related to bone implant technical field, draw lessons from the structural concept of human bone trabecula, complete the preparation of Voronoi porous structure construction and biomimetic Voronoi-nickel titanium skeleton by innovative design method, again with the biomimetic Voronoi-nickel titanium skeleton as base, adopt drying gel method to prepare biomimetic Voronoi-nickel titanium-hydrogel heterostructure implant.The implant integrates the mechanical advantages of high strength, low modulus of nickel titanium alloy and the structural characteristics of high connectivity, high specific surface area of biomimetic Voronoi porous structure, effectively makes up the short board of hydrogel bearing weakness, while hydrogel has buffering energy absorption function, can realize the multi-functional time sequence release of antibacterial, anti-inflammatory, bone regeneration promotion, adapt to the needs of each stage of bone repair.

The material for post-radiation bone repair comprises taurine and a covalent organic framework material, and the taurine is loaded on the covalent organic framework material and then dispersed in a dispersing agent. Through verification, the material disclosed by the invention has good biocompatibility, is in a flowing state when being heated to 52 DEG C and is in a solidification state after being cooled in an oral cavity, endows a bone defect area with retention force, and can stably and slowly release taurine for a long time and promote bone regeneration and repair for a long time in a radiated bone tissue.

The application discloses a kind of biphasic bioactive tissue repair scaffolds and preparation method, comprising the following steps: step 1: calcium phosphate ceramic powder is modified on surface, and the modified calcium phosphate ceramic powder is obtained;Step 2: the modified calcium phosphate ceramic powder, potassium sodium niobate piezoelectric ceramic powder, dispersing agent, photoinitiator, photosensitive resin are mixed evenly, and the light-cured resin slurry is obtained after ball milling;Step 3: according to the design, 3D printing model is constructed, and the light-cured resin slurry is printed according to 3D printing model, and the scaffold body can be obtained;Step 4: scaffold body is cleaned, and the required scaffold can be obtained after defatting sintering;The application can prepare biphasic bioactive tissue repair scaffold with good mechanical properties, efficient antitumor ability and better bone regeneration capacity, and provide technical support for the repair of bone tumor postoperative bone defect in clinic.

To provide a bone regeneration material having both antibacterial properties and high aesthetic properties, and to provide a method for producing the bone regeneration material.SOLUTION: A bone regeneration material in a cotton form comprising a biodegradable fiber, wherein the biodegradable fiber contains calcium phosphate, silver ion-solid solution calcium phosphate, and a biodegradable polymer, and a content ratio of the silver ion-solid solution calcium phosphate to a total amount of the calcium phosphate and the silver ion-solid solution calcium phosphate is 60 mass% or more. The silver ion solid solution calcium phosphate is preferably silver ion solid solution β - tricalcium phosphate.SELECTED DRAWING: None

This invention discloses a method for preparing a strontium titanate modified layer on the surface of zirconia based on an impregnation-heat treatment method, comprising: (1) mixing titanium sulfate, urea and water to obtain a precursor liquid A, placing zirconia ceramic in the precursor liquid A, heating to boiling and maintaining for a period of time, then removing the zirconia ceramic, and after heat treatment A, preparing a titanium dioxide film on the surface of zirconia; (2) mixing H2O2 aqueous solution, ammonia and deionized water, adding metatitanic acid and stirring to dissolve, obtaining a precursor liquid B, placing the zirconia with the titanium dioxide film in the precursor liquid B, and allowing it to stand and react for a period of time at room temperature, depositing zirconia modified with a titanium dioxide precursor layer; (3) dissolving strontium acetate, or a mixed salt composed of calcium acetate and / or magnesium acetate and strontium acetate in deionized water to obtain an impregnation treatment solution, placing the zirconia modified with the titanium dioxide precursor layer in the impregnation treatment solution, allowing it to stand and react for a period of time, and then removing it, and after heat treatment B, preparing a strontium titanate modified layer on the surface of zirconia. The strontium titanate layer prepared on the surface of zirconia ceramic in this invention is tightly attached to the zirconia substrate and has high hydrophilicity, which is conducive to the adhesion of proteins and cells in the human body. At the same time, it can release active factors such as strontium ions for a long time to promote bone formation and bone regeneration at the implantation site, ultimately enhancing the osseointegration capacity of the zirconia implant.

A method for producing biodegradable fibers containing inorganic filler particles by wet spinning, prepared by dissolving a mixture of inorganic filler particles and biodegradable resin in a weight ratio of 50-80:50-20 in a good solvent and stirring the solution is filled into a syringe of a wet spinning machine and extruded downward from the discharge port of the injection needle into a collector container. The collector vessel is filled with a first poor solvent having a dissolution parameter value with a small deviation from the good solvent and a second poor solvent having a specific gravity greater than the first poor solvent and a dissolution parameter value with a large deviation from the good solvent, in two layers, top and bottom. The spinning solution extruded from the syringe outlet enters the first poor solvent in the collector container by its own weight in fibrous form, and then continuously enters the second poor solvent filled below the first poor solvent. The fibrous spinning solution injected into the second poor solvent becomes continuous long fibers and is deposited floating on the bottom of the collector container to form a cotton shape.

The invention belongs to the technical field of biomedical materials and tissue engineering, and particularly relates to a self-pumping stent material with a double-layer heterogeneous structure as well as a preparation method and application of the self-pumping stent material. A hydrophobic membrane is prepared by an electrostatic spinning technology, and a hydrophilic microgel assembly is prepared by an emulsion method. Adding a dopamine modified polymer, and establishing a dynamic phenylboronic acid ester bond between microgel through a dopamine group to form a hydrophilic microgel assembly; the adhesion connection of the microgel assembly and the hydrophobic membrane is realized through mussel bionic chemical reaction of dopamine groups, and a double-layer heterogeneous structure based on the hydrophilic assembly and the hydrophobic membrane is constructed, so that a self-pumping effect is generated, and one-way flow of liquid components from the hydrophobic side of the stent to the hydrophilic side of the stent can be realized. Implanting the stent into an alveolar bone defect wherein the hydrophobic membrane contacts gingiva and the hydrophilic microgel assembly fills the bone defect; through the self-pumping effect of the stent, nutritional ingredients in the gingiva are unidirectionally pumped to the defect part of the alveolar bone, so that the regeneration of the alveolar bone is promoted.

The present application relates to a kind of biomimetic reticular nanofiber composite coating and its preparation method and infectious bone defect repair application, belong to biomedical material field.The present application is aimed at solving the technical problem of the poor bone integration of implant in infectious bone defect.The present application provides a kind of biomimetic reticular nanofiber composite coating, which is formed by layer-by-layer self-assembly on the surface of implant by lysozyme amyloid fiber and polyphenols (such as tannin etc.) Form reticular structure, then prepared with bioactive metal ions coordination;The number of coating layers is 4-8 layers.Preparation method includes lysozyme fiberization, alternate deposition and ion loading steps.The composite coating of the present application has broad-spectrum antibacterial, antioxidant stress and promotes osteogenesis function;In animal model, bacterial load is reduced by 80%, bone volume fraction and trabecular bone parameters are significantly improved, and can realize anti-bacterial biofilm infection-antioxidant stress-angiogenesis-promote bone regeneration synergy.

This invention relates to the field of biomedical materials, and discloses a biomimetic piezoelectric composite membrane, its preparation method, and its application. The biomimetic piezoelectric composite membrane comprises a dense layer and a porous layer. The dense layer contains a first L-polylactic acid and a first D-polylactic acid, while the porous layer contains a second L-polylactic acid, a second D-polylactic acid, and hydroxyapatite. This invention employs a stepwise continuous electrospinning technique to obtain a biomimetic piezoelectric composite membrane with an integrated dense-porous-porous layer structure. The dense layer prevents soft tissue ingrowth into bone defect areas, while the porous layer acts as a porous scaffold, supporting and guiding new bone growth. Simultaneously, it generates electrical signals in response to mechanical stress, promoting osteogenic differentiation. Furthermore, the porous layer is composited with nano-hydroxyapatite, providing essential calcium and phosphorus elements for osteogenic formation. This synergistic effect enhances alveolar bone regeneration and repair capabilities from multiple perspectives, including electrical, chemical, and topological aspects.

The application provides a screening method of human amniotic membrane mesenchymal stem cells (hAMSCs) with bone regeneration promoting potential, and uses the protein level of transmembrane protein 119 (TMEM119) as a functional index of the bone regeneration promoting ability of the hAMSCs, and uses an enzyme-linked immunosorbent assay (ELISA) to detect the TMEM119 protein concentration in a lysis solution prepared from 1×10 7 The application uses the TMEM119 protein concentration not lower than 15000 pg / mL as a screening standard of the hAMSCs with bone regeneration promoting potential, and screens the hAMSCs with significant bone regeneration promoting potential from different batches of hAMSCs, so that the hAMSCs meeting the screening standard of the application have obvious bone formation promoting potential, and the technical problem that the hAMSCs with bone regeneration promoting potential are difficult to be accurately identified and screened in clinical transformation is solved.

The invention relates to the technical field of medical composite materials, in particular to a high-compatibility bioactive glass composite material and a preparation method thereof. The preparation method comprises the following steps: synthesizing Mg / Sr-doped hydroxyapatite nano-powder and Zn / Li-doped bioactive glass microspheres, blending the Mg / Sr-doped hydroxyapatite nano-powder and the Zn / Li-doped bioactive glass microspheres with polycaprolactone, and preparing a stent primary blank with gradient pores which are dense outside and sparse inside through a low-temperature deposition 3D printing technology; and by taking the primary blank as a receiving device and adopting a coaxial electrostatic spinning technology, constructing a core-shell structure nanofiber functional layer loaded with the BMP-2 growth factor on the surface and in internal pores of the primary blank in situ. By regulating and controlling the composition of the bioactive glass and introducing functional metal ions, local alkaline stimulation caused by rapid release of ions after the material is implanted is reduced, and meanwhile, by combining a multi-gradient composite structure design and controllable loading of growth factors, synergistic improvement of biocompatibility, bioactivity and mechanical support performance of the composite material is realized; the bone regeneration can be effectively promoted, the stress damage is reduced, and the method is suitable for bone defect repair.

The invention discloses an exosome and drug loaded gradient aperture periodontal regeneration composite membrane and a preparation method thereof, and belongs to the field of biomedical materials. According to the composite membrane, RGD peptide modified freeze-dried chitosan is adopted as an inner-layer macroporous membrane, silk fibroin loaded with periodontal ligament stem cell source exosome is adopted as a middle-layer mesoporous membrane, silk fibroin loaded with medicine is adopted as an outer-layer microporous membrane, and a three-layer gradient aperture structure is constructed through an electrostatic spinning and freeze-drying combined technology. The interlayer bonding strength is improved through interface spraying of polyvinyl alcohol, the biological activity is guaranteed through an exosome freeze-drying protection technology, and time sequence synergistic release of rapid anti-inflammation of drugs and long-acting regeneration promotion of exosomes is achieved. The composite membrane has excellent mechanical property, degradation property and biological activity, can remarkably promote gingival fibroblast COL-I secretion and alveolar bone regeneration, and is applied to periodontitis tissue regeneration and repair.