688 results about "Bones regeneration" patented technology

The invention relates to the technical field of bone implantation, and discloses a biomechanical evaluation method and system for a bone defect repair implant, and the method comprises the steps: constructing a human muscle-bone system biomechanical model and a three-dimensional digital model of the bone defect repair implant; through a finite element analysis method, the stress conditions of the bone defect repair implant in different gaits and movement modes are calculated, and the stress conditions of the implant in the actual service process are simulated; and calculating the performance of the implant under the simulated stress, and adjusting the design parameters of the implant. By combining dynamics calculation, bone regeneration process simulation, nano computed tomography image three-dimensional reconstruction and structure optimization, a bone repair implant biomechanical adaptability evaluation mechanism is established, and a comprehensive evaluation system integrating system dynamics calculation and bone healing process analysis is designed. The bone repair and regeneration process and the acting force change process are simulated, the effect of mechanical stimulation on bone regeneration is revealed, and comprehensive evaluation of the structure, the mechanical property, the environmental adaptability and the service life of the implant is achieved.

Hydrogel comprises hydroxyapatite nanowires and a base material for forming the hydrogel, and further comprises glucose oxidase (GOx), gallic acid, magnesium ions (Mg < 2 + >) and the like, the gallic acid and the Mg < 2 + > form Mg-MOF, and the GOx is loaded on the Mg-MOF, so that the diabetes microenvironment of a bone defect part is locally adjusted, and bone defect repair is facilitated. Through verification, the sustained-release gallic acid of GOx-MOF participates in cascade reaction, GOx is removed to generate H2O2, the benefit is maximized, and the sustained-release gallic acid has good effects of controlling blood glucose, removing ROS, regulating inflammation and promoting osteogenesis and angiogenesis. The research is expected to play a role in microenvironment regulation of diabetes mellitus bone regeneration and minimally invasive treatment of bone defects.

The invention belongs to the technical field of hydrogel, and relates to piezoelectric / conductive integrated hydrogel for jaw defect repair, which is prepared from methylacryloyl protein, zwitterionic monomer and titanate piezoelectric particles through ultraviolet crosslinking under the action of a crosslinking agent and a photoinitiator, the hydrogel comprises, by mass, 1.0%-10% of methacrylated protein, 1.0%-10% of zwitterionic monomers, 1.0%-10% of titanate piezoelectric particles and 0.1%-5% of an initiator, and the volume fraction of an accelerant is 0.05%-1.0%. The hydrogel disclosed by the invention has excellent mechanical strength and certain flexibility, can realize piezoelectric and conductive functions of the hydrogel, can effectively promote proliferation, differentiation and mineralization of bone cells under the action of an electric field, accelerates bone tissue repair, and has a more remarkable bone regeneration effect compared with a non-electrical stimulation material in the prior art.

The invention belongs to the field of biomedical materials, and particularly discloses a preparation method and application of a 3D printing porous composite scaffold. I-type collagen and silk fibroin composite gel is modified on a 3D printing three-element doped hydroxyapatite / polycaprolactone (PCL) composite scaffold with a polydopamine coating, the three-element doped hydroxyapatite is synthesized by using a chemical precipitation method, and Mg < 2 + >, Sr < 2 + > and SeO3 < 2-> ions are doped in crystal lattices. The 3D printing porous composite scaffold provided by the invention effectively simulates the structure and function of soft and hard combination of natural bones, is helpful for regulating and controlling the stem cell fate to improve the bone regeneration process, repairs bone defects while eliminating tumors, and can be applied to bone defect repair or osteosarcoma treatment.

The invention belongs to the field of medical materials. The invention provides a porous artificial bone and a preparation method thereof, the components of the porous artificial bone comprise absorbable macromolecules and an orthopedic inorganic implant material, the mass ratio of the absorbable macromolecules to the orthopedic inorganic implant material is 1: 0.1-2, the porous artificial bone has a through porous structure, the porosity is greater than 70%, and the pore size distribution range is less than 500 [mu] m. The porous artificial bone has a pore structure and mechanical properties beneficial to bone regeneration, the pore diameter is stable, the mechanical properties are appropriate, after the porous artificial bone is implanted into a defect part, the bone volume is always increased, the increase rate of regenerated bone tissue is remarkably improved, the effect of bone conduction is effectively achieved, bone regeneration is very beneficial, and animal experiments prove that the porous artificial bone has a good application prospect. The material can be used for filling and repairing various bone defects in orthopedic surgery. The preparation method is simple, stable and effective, can be absorbed, is low in cost, has great market potential, and is simple, stable and effective compared with a conventional artificial bone preparation method at present.

The invention discloses functional injection type bionic hydrogel for promoting bone healing and a preparation method thereof, and relates to the technical field of medical instruments. The hydrogel has good functional characteristics, is a biomimetic material prepared on the basis of methacrylated gelatin, and aims to regulate and control the degradation rate, the drug release mechanism and diversified synergistic treatment and promote bone repair and regeneration; according to the hydrogel, matching of the degradation rate and the bone regeneration rate is achieved through reasonable matching, stem cell osteogenic differentiation and angiogenesis are promoted, ultrasonic controlled-release drug release and synergistic controlled-release of active factors are achieved, inflammation is reduced, and the bone repair process is promoted; the calcium phosphate bone cement provides biocompatibility and bone conductivity, and the nano clay releases active ions to improve the microenvironment and promote bone repair; in addition, the magnetic nanoparticles and a static magnetic field have synergistic interaction, the bone healing process is further accelerated, and treatment effect monitoring is enhanced through magnetic resonance imaging.

Aiming at the challenge of osteochondral defect repair, the invention provides a preparation method of a fiber-reinforced self-repairing hydrogel scaffold (SBPS). According to the method, phenylboronic acid modified sodium alginate, polyvinyl alcohol and fibroin nanofibers rich in beta-Sheet are utilized to form the hydrogel through dynamic boric acid ester bonds and hydrogen bonds. The SBPS hydrogel loaded with PDGF-BB is combined with the nanosheet containing LDH / ChS / TGF-beta3, so that multi-dimensional synergy of mechanical strengthening, sequential drug release (PDGF-BB is released in an early stage and TGF-beta3 / Mg < 2 + > is released in a later stage), ROS removal and endogenous repair is realized. The Mg < 2 + > concentration gradient effect further assists staged regulation (vascularized bone regeneration-cartilage matrix synthesis), and breaks through the limitation of a single factor. Experiments prove that the scaffold can effectively drive angiogenesis, stem cell recruitment and cartilage / bone differentiation in vivo and in vitro, and finally osteochondral regeneration with structure-function matching is achieved.

The invention relates to the field of biomedical materials, in particular to composite hydrogel as well as a preparation method and application thereof. The composite hydrogel prepared by the invention realizes step-by-step release and time sequence regulation of a polypeptide drug through the combined action of Gel-PBA and GelMA: the anti-inflammatory peptide (Ac2-26) is quickly released under the conditions of high ROS and low pH, so that inflammation and pyroptosis are reduced; osteogenic peptide (OGP) is continuously and slowly released, and osteogenic differentiation is promoted. The step-by-step release mechanism of the anti-inflammatory peptide and the osteogenic peptide can realize accurate drug release, and is helpful for synergistically regulating inflammation and promoting bone regeneration. The composite hydrogel is used as a biological medicine material, can realize timely anti-inflammatory effect and long-term osteogenesis in a complex pathological environment such as diabetic periodontitis, and has a good application prospect.

The invention discloses a bone regeneration promoting drug-loaded skull implant with a bionic structure and a preparation method of the bone regeneration promoting drug-loaded skull implant, and belongs to the technical field of medical materials. The skull implant is prepared through a flat plate mold pressing technology and is of a bionic three-layer structure, the upper layer and the lower layer of the haft tube imitating structures and the middle layer of the bone trabecula imitating structure are prepared through the water-soluble core, a vascularization channel can be provided, and bone tissue climbing is promoted; a polydopamine-carboxymethyl chitosan coating is used for carrying out surface modification on a porous structure of the implant, hydroxyapatite in-situ forming and bone regeneration promoting medicine loading are achieved through an impregnation process, and the polydopamine coating can provide a hydration environment; hydroxyapatite in-situ forming increases biocompatibility on the premise of not influencing the mechanical properties of implants, in addition, hydroxyapatite degradation and drug release can jointly induce bone tissue regeneration, and skull repair is promoted.

The invention discloses an injectable material for promoting bone regeneration through piezoelectric and conductive integrated nanofiber hydrogel with ultrasonic response and a preparation method of the injectable material. The injectable material is composed of piezoelectric and conductive nanofibers and hydrogel. Wherein the piezoelectric conductive nanofiber is prepared from an inorganic piezoelectric material and a piezoelectric polymer through electrostatic spinning, or is prepared from an inorganic / organic composite piezoelectric material and a biological material with conductive performance through electrostatic spinning.

The invention relates to a method for constructing an injectable bone organ to realize rapid bone regeneration. The method comprises the following steps: 1, obtaining a water-soluble periosteum acellular matrix by adopting an acellular technology; secondly, compound bone salt is prepared according to the proportion of natural bone salt components, the water-soluble periosteum acellular matrix and the compound bone salt are mixed according to the proportion of natural bone organic and inorganic components to form a bionic matrix component, and then the bionic matrix component is added into gelatin to construct a completely bionic osteogenesis microenvironment; and 3, constructing a bone regeneration material to form a bone organ, firstly preparing a granular gelatin scaffold with a full-bionic osteogenesis microenvironment, then inoculating the bone marrow mesenchymal stem cells, finally performing in-vitro culture, and regulating the differentiation and secretion of the bone marrow mesenchymal stem cells by virtue of the osteogenesis microenvironment of the scaffold to finally form the bone organ.

The invention provides an interface-enhanced regenerated bone scaffold for sustained-release medicines as well as a preparation method and application of the interface-enhanced regenerated bone scaffold, and belongs to the technical field of bone tissue engineering. According to the invention, polycaprolactone and hydroxyapatite are taken as matrix components of the regenerated bone scaffold, so that the regenerated bone scaffold has mechanical strength matched with a host bone and good biocompatibility; the polydopamine coating is loaded on the surface and internal pores of the regenerated bone scaffold matrix, surface functional modification is easy to perform, and a catechol structure in the polydopamine coating can perform coordination binding with metal ions in MOFs, so that the falling rate of MOFs particles is reduced, and the MOFs particles are uniformly and stably distributed; the MOFs particles are used for loading the eucommia ulmoides extract, safety and non-toxicity are achieved, the eucommia ulmoides extract is encapsulated in the MOFs particles, the stability of the eucommia ulmoides extract can be improved, and a better drug release effect and a better bone regeneration promoting effect are achieved.

The invention belongs to the crossing field of biomedical engineering and additive manufacturing, and particularly relates to a 3D printing metamaterial piezoelectric bone defect repair scaffold, and the pore structure of the scaffold is formed by compounding three extremely small curved surface units, namely Gyandroid, SchwarzP and Diamond, which are defined by mathematical expressions; the porosity of the stent is 30%-70%, and the pore diameter range is 0.1-1 mm; the stent is made of a piezoelectric composite material composed of polyvinylidene fluoride (PVDF), polylactic acid and barium titanate, the piezoelectric composite material comprises the following components in percentage by mass: 30%-55% of polyvinylidene fluoride (PVDF), 20%-45% of polylactic acid (PLA) and 10%-25% of barium titanate (BaTiO3), and the sum of the mass percentages of all the components is 100%. Precise regulation and control of a macrostructure and a microstructure are achieved through a multi-material 3D printing technology, and the system has the mechanical adaptability, degradation controllability and the function of actively promoting bone regeneration and is suitable for large-section bone defect repair scenes such as craniomaxillofacial and long bones of four limbs.

The invention belongs to the technical field of bone repair hydrogel, and discloses a hydrogel band-aid for bone repair and a preparation method and application thereof, and the preparation method comprises the following steps: mixing a polyethylene glycol succinimide succinate solution with an alendronate solution, dialyzing, and freeze-drying to obtain alendronate modified polyethylene glycol succinimide succinate; and uniformly mixing alendronate modified polyethylene glycol succinimide succinate, polyphenol, metal ions and water in proportion, carrying out solid-liquid separation, and drying the solid to obtain the hydrogel. The preparation process provided by the invention has the advantages of simplicity, convenience and rapidness, not only simplifies the production process, but also enhances the bone regeneration, angiogenesis and immunoregulation capabilities.

The invention discloses a piezoelectric double-network hydrogel coating modified titanium material and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The piezoelectric double-network hydrogel coating modified titanium material provided by the invention comprises a titanium substrate, an adhesion layer and a piezoelectric double-network hydrogel coating, wherein the adhesion layer and the piezoelectric double-network hydrogel coating are compounded on the titanium substrate; the adhesion layer is compounded on the titanium substrate, and the adhesion layer is positioned between the titanium substrate and the piezoelectric double-network hydrogel coating; and the piezoelectric double-network hydrogel coating is prepared from methacrylic acid modified gelatin, sodium alginate and whitmanite nanoparticles. According to the prepared modified titanium material, electric signals can be generated on the surface of the piezoelectric material under the action of polarization or stress, recovery of charge transfer of a bone defect part is facilitated, a good electroactive microenvironment is created for bone regeneration, the osseointegration capacity of the modified titanium material is enhanced, and bone regeneration is effectively promoted; the method has a wide application prospect in preparation of bone graft materials and bone repair materials.

Biomaterials disclosed herein can comprise a hydrogel comprising PEG, gelatin, and a glycosaminoglycan with sulfated moiety; and chondrogenic, osteogenic, and immunomodulatory cytokines; wherein the biomaterial is capable of potentiating bone regeneration in a compromised wound while reducing inflammatory response. The glycosaminoglycan with sulfated moiety can comprise heparin, heparan sulfate, keratin sulfate, chondroitin sulfate, dermatan sulfate, and / or similar materials. The biomaterial can further comprise mesenchymal stem cells (MSCs), a crosslinking initiator, microparticles and nanoparticles, and or other materials. The biomaterial can be injectable into a wound, or the biomaterial can be loaded in, or further comprise a porous scaffold providing mechanical support for other components of the biomaterial, such that it can be implanted into a wound.

The invention discloses a chitosan / nano-hydroxyapatite guided bone regeneration membrane with a density composite structure and a preparation method of the chitosan / nano-hydroxyapatite guided bone regeneration membrane. Chitosan is used as an organic matrix, calcium salt and phosphate are induced in situ through a freeze-drying method to be uniformly mineralized and crystallized on the surface of a chitosan polymer chain to form nano-hydroxyapatite, and the chitosan / nano-hydroxyapatite guided bone regeneration membrane with a density composite structure is prepared in combination with a mechanical extrusion method. And oriented crystallization and uniform dispersion of the nano-hydroxyapatite are realized through in-situ induction by virtue of rich functional groups (amino groups) of chitosan, so that the bone mineralization capability is enhanced through nanomechanics. The compact layer of the membrane effectively prevents tissue cells growing fast from entering a tooth root (bone) defect area, the loose porous layer guides periodontal ligament (or bone) cells to collect and provides growth space for the periodontal ligament (or bone) cells, and the optimal tissue regeneration effect is ensured. The invention has the advantages of wide raw material source, cheap price, simple process, environmental protection and easier conversion of finished products, and is expected to be applied to clinical dental bone defect repair.

The invention discloses an electroactive dressing used for promoting bone cell differentiation and bone regeneration and used for negative pressure therapy and a manufacturing method. The manufacturing method of the electroactive dressing comprises the steps that S1, a PLLA / BG spinning solution is prepared; s2, preparing a PLLA / BG nanofiber membrane through electrostatic spinning; s3, vacuum drying; s4, PAMAM-NH2 surface modification, the negative pressure treatment dressing relates to the technical field of negative pressure treatment dressings, when mechanical deformation is generated during negative pressure work, the piezoelectric biological film generates continuous electric stimulation, and the electric stimulation has positive and beneficial effects on osteoblasts and bone tissue physiological processes. The generated electric effect continuously collects functional electrolyte in surrounding tissues, and the functional electrolyte is separated in the wound by the dendrimer PAMAM-NH2, so that excessive exudation caused by negative pressure treatment is effectively made up. Meanwhile, the flow of the electrolyte further strengthens a local electric signal, so that cell migration and osteoblast proliferation and differentiation are effectively promoted.

The invention relates to a preparation method and application of a functional gradient layered bone-imitating GBR (Guarantee Boron Reactor) membrane, the GBR membrane is a novel GBR membrane obtained by taking decellularized fish scales as a base material, constructing an EGCG-fish scale complex through EGCG cross-linking modification and then combining biomimetic mineralization, and the GBR membrane has certain mechanical properties and can maintain a space required by bone regeneration; meanwhile, mineralized hydroxyapatite wraps fish scale collagen, so that the degradation speed of fish scales is reduced, the action time is prolonged, and sufficient time is provided for bone repair; in addition, the mineralized fish scales are similar to natural cortical bones in structure and composition, a cell growth microenvironment is simulated, good biocompatibility is achieved, adhesion and differentiation of osteoblasts are promoted, and the healing speed is increased.

The invention relates to the technical field of tissue engineering, and particularly discloses preparation of an exosome and electrostatic spinning composite material for promoting bone regeneration and application of the exosome and electrostatic spinning composite material in bone tissue regeneration. Identifying the multiplication capacity, the surface marker and the multidirectional differentiation capacity of the cells after the cells are separated and cultured, and finally extracting and identifying the exosome; pLLA and gelatin are dissolved in a cosolvent trifluoroethanol, composite fibers are prepared through electrostatic spinning, and a composite fiber membrane is obtained through spinning by adopting a receiver; the exosome is loaded on the surface of the PLLA / gelatin composite fiber membrane. According to the invention, the exosome derived from human mesenchymal stem cells is combined with the membrane material to prepare the exosome capable of stably releasing, the good stability and low immunogenicity of the exosome are exerted, and the effect of accurately promoting local bone regeneration is realized. And defect repair and functional reconstruction are realized.

The invention belongs to the technical field of biomedical materials, and particularly relates to application of a HAp-MXene nano composite material in regulation and control of an osteogenesis immune microenvironment. The HAp and MXene nanocomposite is obtained by combining hydroxyapatite nanorods and MXene nanosheets, the HAp and MXene nanocomposite is interfered by adjusting an external magnetic field, so that the HAp and MXene nanocomposite shows one effect of regulating and controlling polarization of macrophage M1 or macrophage M2; then, the HAp-MXene nanocomposite is implanted into a bone defect area, and in the initial stage of the operation, the magnetoelectric effect of MXene is excited by applying a rotating magnetic field, polarization of M1 type macrophages is promoted, proinflammatory factors are generated, and bacterial infection is inhibited; then, in the middle stage, through removing a magnetic field, releasing calcium ions, promoting polarization of the M2 type macrophages, relieving inflammatory response and promoting bone regeneration, programmed regulation and control of the macrophages are achieved in different repair stages.

The invention belongs to the technical field of medical materials, and particularly provides a 3D printing bone repair material and a preparation method thereof.The 3D printing bone repair material is prepared from PCL / MnO2 composite microspheres and SiN, PCL has good biocompatibility and biodegradability, the mechanical performance and bone induction performance of the 3D printing bone repair material are enhanced due to addition of SiN, and the 3D printing bone repair material has a good application prospect. MnO2 can release oxygen and manganese ions, and the manganese ions can promote calcium deposition, jointly promote cell infiltration, osteogenic differentiation and matrix mineralization, promote bone precursor cell proliferation, accelerate bone defect repair and promote bone regeneration, so that the bone regeneration rate is increased, and the bone regeneration rate is increased. The prepared 3D printing bone repair material has good mechanical performance, biocompatibility, antibacterial property and biodegradability, cell proliferation is promoted, and bone repair and bone regeneration are accelerated.

The invention discloses a special-shaped titanium alloy capable of enhancing bone regeneration and repair efficacy as well as a preparation method and application of the special-shaped titanium alloy. The special-shaped titanium alloy comprises a special-shaped titanium alloy base body and a porous structure attached to the special-shaped titanium alloy base body, and the porous structure is in multi-stage size distribution and comprises long-strip-shaped gullies (100-150 micrometers wide) and large holes (30-50 micrometers wide). And the morphology in the gullies shows coralline ligaments and a nest-shaped pore channel (0.5-2 microns) structure. This can provide space and sites for the growth and adsorption of osteoblasts. According to the method, a gas phase alloying (VPA) and VPD synergistic process is adopted in a breakthrough mode, a non-oxidation / nitridation coralline-shaped hierarchical pore structure is constructed on the surface of the commercial titanium implant, and it can be achieved through verification of an aging animal model that a special-shaped matrix is uniformly modified in the whole domain, the regeneration function of aging stem cells is activated, and the bone repair and regeneration effect is effectively improved.

The invention relates to a pH-responsive copper hydrogen phosphate-loaded hydrogel with multiple enzyme-like activities and a preparation method and application thereof. The material mainly shows catalase-like activity in a neutral microenvironment, and can remove H2O2 and relieve oxidative stress; in a weakly acidic microenvironment, the compound mainly shows peroxidase-like activity and catalyzes H2O2 to generate. OH bacteria with higher toxicity; meanwhile, the inherent osteogenesis promoting performance of copper synergistically enhances bone regeneration, the biocompatibility, the oxidation resistance, the osteogenesis promoting capacity under the oxidative stress condition and the antibacterial efficiency under the weak acid condition of the SA / CuHP hydrogel are evaluated through in-vitro experiments, and a type II diabetes rat periodontitis model is established; the in-vivo antibacterial and osteogenesis promoting effects and the biological safety performance of the compound are verified, and a new strategy is provided for treatment of periodontitis with diabetes mellitus.

The invention discloses a muscle-bone regeneration sustained-release preparation for targeting knee joint cartilage and a preparation process thereof, the preparation is composed of a cartilage targeting peptide modified PLGA-hyaluronic acid composite nanoparticle sustained-release carrier, an active regeneration component, a biocompatible matrix and an anti-inflammatory component, nanoparticles are prepared by a multiple emulsion solvent evaporation method, and targeting peptide is modified; the composite concentrated growth factors, stem cells and traditional Chinese medicine extracts have the functions of targeted enrichment, long-acting slow release, anti-inflammation and cartilage regeneration promotion, and can be used for knee joint cartilage defect repair.

The present invention discloses an L-shaped fiber supramolecule and its preparation method and application. The structural formula of the L-shaped fiber supramolecule is shown in formula (I). The preparation method includes: activation of resin, coupling of amino acids, excision and precipitation of polypeptides, and separation and purification of polypeptides. The L-shaped fiber supramolecule prepared by the present invention has good biocompatibility, does not cause inflammatory reaction at the wound site, has the function of binding calcium ions, and exhibits excellent effects in hemostasis and bone regeneration. #imgabs0#

The invention provides a composite material porous bone repair scaffold as well as a preparation method and application thereof. The composite material porous bone repair scaffold is prepared from the following raw materials: a bone repair organic material, a bone repair inorganic material and poly (citric acid-1, 8-octylene glycol / ethylene glycol) ester particles. The biodegradable poly (citric acid-1, 8-octylene glycol / ethylene glycol) ester particles are added into the bone repair organic material and the bone repair inorganic material, so that the degradation rate of the bone repair scaffold can be regulated and controlled, and the degradation rate of the composite material is matched with the new bone regeneration rate; moreover, the bone repair scaffold has good biocompatibility and high osteogenic activity, the mechanical property of the bone repair scaffold can be improved, the mechanical behavior of natural bones can be simulated, and the stability of the scaffold can be improved.

The invention belongs to the technical field of dental dressings, and particularly relates to a bacteriostatic repair-promoting hydrogel dressing and a preparation method thereof. It is found for the first time that the compound A has remarkable antibacterial activity on common bacteria in the oral cavity, and after the compound A is prepared into the temperature-sensitive hydrogel dressing, the compound A still has antibacterial activity. When the implant is injected into the periphery of the implant, the alveolar bone can be prevented from being reduced, vertical bone regeneration at the periphery of the implant is stimulated, the bone mass is increased so as to improve the stability of the implant, finally, the fusion of a wound between the alveolar bone and the implant is promoted, and the service life of the implant is prolonged.

The invention discloses a preparation method and application of shape memory short fiber stiffened hydrogel, and the preparation method comprises the following steps: obtaining a copolymer PLCL nanofiber membrane of poly-L-lactic acid and caprolactone through electrostatic spinning, carrying out stretching shaping and cutting at a certain temperature, dispersing in a low-temperature homogenizer to form shape memory short fibers SMSFs, and carrying out shape memory short fiber reinforcement on the shape memory short fibers SMSFs to obtain the shape memory short fiber hydrogel. The SMSFs are added into the double-network hydrogel, and the shape memory short fiber stiffened hydrogel is obtained by exciting the shape memory effect of the SMSFs. The invention relates to an application of a shape memory short fiber, which is used for preparing shape memory short fiber stiffened hydrogel for promoting osteogenic differentiation and inhibiting osteoclast generation. According to the shape memory short fiber stiffened hydrogel and the application method thereof, the stiffening effect of the shape memory short fiber stiffened hydrogel has the direct regulation and control effect on stem cell osteogenic differentiation and indirectly has the ability of inhibiting osteoclast formation, and a new way is provided for preparing a bone regeneration scaffold for osteoporotic bone defect repair.

The invention relates to a 3D printing porous metal-ceramic assembled composite implant as well as a preparation method and application thereof. The 3D printing porous metal-ceramic assembled composite implant comprises a porous metal-ceramic implant body formed by compounding a 3D printing integrated porous metal module and a 3D printing integrated porous ceramic module, the porous metal-ceramic implant body and the net-shaped metal protection body are arranged on the periphery of the porous metal-ceramic implant body in a wrapping mode, and the assembled composite implant can be accurately matched with the appearance and the combination surface of bone tissue of a defect part. The composite implant disclosed by the invention has osteogenesis environment regulation and control functionalization, and can achieve the effects of immediately recovering the movement function of defective bone tissue, improving the tissue microenvironment of a bone defect part, accelerating new bone regeneration and guiding new bone to grow towards the interior of the porous metal implant, so that rapid osseointegration of a bone / metal implant interface is realized; and the long-term treatment effect of 3D printing porous metal implant / autologous bone fusion type repair after ceramic degradation is achieved.