14 results about "Tissue formation" patented technology

The application provides a preparation method of organoids based on a single cell chip, which comprises microfluidic chip preparation, a two-water-phase solution preparation, a single cell suspension preparation, microfluidic manipulation, single cell-loaded microgel generation and organoid construction and the like. The application takes a microfluidic chip integrated with a normally closed pneumatic pump valve as a technical platform, takes a single cell suspension pre-incubated with a crosslinking agent as a chemical reaction core, takes a water-in-water droplet as a forming template, and realizes efficient in-situ loading of single dispersed seed cells in a micro hydrogel carrier through a one-step method, and simply removes empty droplets without cells in a subsequent transfer step. The single cells in the microgel can be cultured, expanded and self-organized to form required organoids. The application improves the consistency of the starting point of the organoids, has the high-throughput characteristics of the droplet microfluidic technology, realizes efficient and highly reproducible in-vitro construction of the organoids, and can play a great role in the fields of basic research and transformation application related to the organoids.

The linear cutting anastomat with the side plate comprises a nail bin seat and a nail abutting seat, the nail abutting seat is movably hinged to the nail bin seat, a nail bin assembly is arranged on the nail bin seat, a knife pushing groove used for a cutting knife to walk is formed in the nail bin assembly, a nail bin is arranged on the position, on one side of the knife pushing groove, of the nail bin assembly, and the side plate is arranged on the nail bin assembly. The nail bin is provided with a nail outlet hole, the other side of the push broach groove is provided with a first clamping part, the nail abutting seat is provided with a nail abutting part and a second clamping part, the nail abutting part corresponds to the nail outlet hole of the nail bin, and the second clamping part corresponds to the first clamping part; a side plate is connected to the position, on one side of the nail bin, of the nail bin base, and a gap is formed between the side plate and the nail bin base. The invention further provides an eversion type extra-cavity anastomosis method based on the anastomat. By arranging the side plate structure, physical limiting is formed on the tissue, so that the tissue to be cut is effectively prevented from sliding towards the retention side in the cutting process, it is ensured that the tissue is cut neatly, the anastomotic stoma area is clean, and the anastomotic effect and the overall quality of an operation are improved.

The application discloses a kind of wound repair composite preparation based on FGF2 variant and its application, it is related to the technical field of skin wound repair biological preparation, the composite preparation is composed of active ingredient and matrix, the active ingredient is FGF2 mutant, the matrix is cream.The FGF2 mutant is NGF2 protein, the NGF2 protein is obtained by protein engineering technology to natural FGF2 is reformed;The NGF2 protein has high thermal stability and extended normal temperature half-life.The cream is preferably water cream (Basic Aquacream).In the application, NGF2 and water cream synergistically, extend the local action time of protein, realize the effect of reducing the frequency of smearing, enhance repair durability.Preparation can effectively promote wound granulation tissue formation, angiogenesis and collagen remodeling, improve healing quality and reduce scar, provide reliable scheme for the development of new generation wound repair biological preparation.

Provided is an implantable tissue scaffold comprising a mixture of a biocompatible organic polymer and chitin, wherein the chitin is embedded in the biocompatible organic polymer. Also provided is a composition for 3D printing, the composition comprising at least one biocompatible organic polymer and chitin that may be partially deacetylated, wherein the chitin is embedded within the biocompatible organic polymer. Further provided is a method of promoting tissue formation, comprising implanting a tissue scaffold comprising a mixture of a biocompatible organic polymer and chitin at a site in need of regenerative bone tissue formation.

The present invention relates to a reverse retreat lithotripsy device. The reverse retreat device can include a lithotripter, a collection channel, and an energy directing device. The lithotripter can be configured to deliver energy to tissue at a tissue formation site. The collection channel can be positioned at or near a body lumen. The energy directing device can be positioned near the lithotripter and the collection channel. The energy directing device can be configured to propel tissue toward the collection channel.

To provide a method for producing a cell tissue, capable of reducing variation in application amount among a plurality of application parts.SOLUTION: The depth of the coated surface of the first substrate for cellular tissue formation relative to the surface is measured. Prior to supply of the cellular tissue to the first substrate, a first trial application of a first application liquid is performed on at least one application part that is a part of an application surface of the first substrate (S3). In the step of performing the first trial application (S3), the first application liquid is applied to one application portion by the first pushing amount of the application needle in accordance with the depth (S3 - 1). A first measuring step (S3 - 3) of measuring the diameters of the first coating liquids is performed.SELECTED DRAWING: Figure 11

Provided are ultrafine-fiber or microfiber scaffolds including a plurality of wall fibers and a plurality of reinforcing fibers, each reinforcing fiber including at least two (such as at least four) anchor segments and at least one (such as at least three) bridge segment, wherein: the wall fibers and the anchor segments are substantially aligned forming a plurality of walls, and the bridge segments each extend across the space between any two adjacent walls. Further provided are methods of producing an ultrafine-fiber or microfiber scaffold by electrohydrodynamic printing (EHD), including jetting along a first straight jetting path that is offset relative to the wall fiber direction at angle of less than 90°. Also provided are methods of culturing cells, forming tissues, and treating an injury using the disclosed scaffolds and compositions.

Provided is a cell culture suitable for use in the repair of a cartilage.　The present invention provides a cell culture having a chondroid tissue formation property, the cell culture comprising a cell mass in which the expression level of at least one cell surface marker selected from the group consisting of CD166, CD165, CD99, GD2, STRO-1, CD108, CD164, CD6, CD106 and CD107b is equal to or lower than a threshold value for each of the cell surface markers, and / or the expression level of at least one cell surface marker selected from the group consisting of CD26, CD73, CD105, CD44, CD120a, CD201, EGFR, CD146, CD140a and CD90 is equal to or higher than a threshold value for each of the cell surface markers.

Stretchable electronics implanted during tissue formation allow chronic studies of tissue-wide electrophysiology. This disclosure describes integrated, stretchable mesh nanoelectronics implanted during organogenesis of human stem cell-derived pancreatic islets, allowing for the long-term, stable recording of single-cell extracellular spike bursting dynamics. By employing a conventional spike sorting pipeline, two major electrical states for both α and β cells were identified based on their responses to glucose threshold for action potential firing. Continuous recording during further maturation by entrainment to 24-hour feeding cycles showed islet-level hormone secretion rhythms reflect increased and sustained coordination between α and β stimulated electrical activities, linked to cell-cell communication and exocytic network induction, revealing a role for circadian rhythms in coordinating system- level stimulus-coupled responses. These results highlight the ability for "cyborg" islets to advance efforts to generate fully functional in vitro islets for research and therapeutic applications.

The invention belongs to the technical field of artificial intravascular stents, and particularly relates to a small-caliber tissue engineering intravascular stent and a preparation method and application thereof. The method comprises the following steps: 1) doubling and knitting silk yarns and polylactic acid fiber yarns by using a knitting machine to obtain a tubular fabric; 2) performing heat setting to obtain an intravascular stent skeleton; (3) putting the intravascular stent skeleton into a mold filled with the gel liquid, and freeze-drying the intravascular stent skeleton; (4) crosslinking; and (5) purifying the cross-linked material to obtain the small-caliber tissue engineering intravascular stent with the inner diameter of 2-6mm. According to the invention, fibers with different degradation speeds are adopted to weave the vascular skeleton, polylactic acid is high in degradation speed, and rapid degradation is used for providing a growth space for cells; the silk is low in degradation speed and plays a role in supporting and enhancing; meanwhile, the chitosan and the gelatin hydrogel play a role in promoting cell adhesion in a scaffold texturing process.

The depth, relative to a surface, of an application surface of a first substrate for cell tissue formation is measured. Prior to the supply of a cell tissue to the first substrate, at least one application section, which is a section of the application surface of the first substrate, is subjected to first trial application (S3) with a first application liquid. In the step (S3) for the first trial application, the first application liquid is applied (S3-1) to the one application section using a first pressing amount of an application needle, said first pressing amount corresponding to the depth. A first measurement step (S3-3) for measuring the diameter of the first application liquid is performed.

The invention relates to a plaster patch for forming photodynamic reaction based on tissue and a manufacturing method thereof. The plaster patch comprises a plaster skin layer, a plaster layer, a stripping protection layer, a light source exciter, a chip board and a plurality of luminous bodies. On one hand, photodynamics and plaster are integrated, light beam elements are relatively separated by the plaster layer, wavelength at a strong absorption peak of a medicament in the plaster layer forms tissue to generate photodynamic reaction so as to promote efficient absorption of the medicament, and hot compress caused by light waves reduces discomfort in use; on the other hand, the pasty fluid can wrap and hide the light beam elements, the light beam elements are arranged in a relatively separated mode, the photodynamic reaction can be prevented from being affected by the external environment, meanwhile, the light beam elements can be repeatedly used, the structure is simple, the manufacturing cost and the use cost are low, and practicability is high.

A device can include a medical treatment system including an implantable scaffold and components that can be configured to be coupled with a target site. The implantable scaffold can include one or more surfaces having a surface architecture with a texture that can promote formation and regeneration of tissue at a target site. The formed tissue can have a similar texture to a corresponding surface of the implantable scaffold.

The invention discloses a puncture structure, and belongs to the technical field of medical instruments, the puncture structure comprises a cannula assembly, the cannula assembly comprises a circuit layer and a heating layer, the circuit layer is located in the heating layer, the heating layer is arranged around the circuit layer, the circuit layer and the heating layer are spaced from each other, and the heating layer is used for heating a wound surface. A heat dissipation channel is defined between the heating layer and the circuit layer; the connecting piece is located in the heat dissipation channel and electrically connected with the circuit layer and the heating layer. When the puncture structure is used, tissue can be punctured to cause bleeding of the tissue on a moving path, the temperature on the heating layer is raised to a proper range through the circuit layer, in the moving process of the device, the bleeding tissue in contact with the path can be heated for hemostasis, the hemostasis effect is good, a current loop cannot be formed through the tissue, and the hemostatic effect is good. The injury to a patient is small, and the safety is improved.