The invention discloses a hot stamping foil applied to overlapping hot stamping on a gold stamping layer and a preparation method of the hot stamping foil. When overlapping hot stamping is carried out on an existing hot stamping foil, the defects that the transferring is incomplete, the hot stamping is not carried out on some portions due to omission, scumming is caused, powder falls off, and the edge of a hot stamping layer is not clear can happen. The hot stamping foil is composed of a base membrane layer, a release layer, a color coating layer, a vacuum aluminum coated layer and a gumming layer. The gumming layer is prepared by raw materials which comprise, by weight, 1-6 parts of chloroethylene-vinyl acetate resin, 1-10 parts of polyvinyl alcohol, 1-10 parts of acrylic ester, 1-5 parts of nitrocellulose, 1-5 parts of fumed silica, 20-70 parts of ethyl acetate, 10-50 parts of ethyl alcohol and 1-5 parts of propyl acetate. According to the hot stamping foil applied to the overlapping hot stamping on the gold stamping layer, the hot stamping foil has the good stamping performance and the good trimming performance, the overlapping hot stamping layer is complete in transferring, the edge is smooth and free of burrs, the adhesion is firm, the phenomena of dusting, powder falling and the like do not exist, the hot stamping effect is strong in third dimension, and the anti-fake performance is more outstanding.