A stereoscopic imaging optical device and a culture bin

Abstract

The application discloses a kind of stereoscopic imaging optical device and culture bin, wherein a kind of stereoscopic imaging optical device is disclosed, including light source module, light processing module, sample placement platform for placing sample and imaging module arranged in sequence, the light beam emitted by the light source module is irradiated and passes through sample placement platform after light processing module, the light beam that passes through sample placement platform enters imaging module and is imaged;The included angle between the light beam that enters sample placement platform and the imaging optical axis of sample placement platform is α, and the α is 15-25 °;The application also discloses a kind of stereoscopic imaging culture bin, using the stereoscopic imaging optical device described above, sample placement platform is located in the chamber of culture bin, imaging module and culture bin are detachably connected, light source module and light processing module are located in culture bin, light path is bent using reflector to adapt the shape and size of culture bin.

Description

Technical Field

[0001] This invention relates to the field of optics, and in particular to a stereoscopic imaging optical device and a culture chamber. Background Technology

[0002] During embryo culture, it is necessary to observe the embryo to obtain information that can determine its developmental status. However, as a typical transparent biological sample, embryos are generally poorly distinguishable under conventional bright-field illumination due to the lack of ability to differentiate transparent structures. This often results in problems such as low image contrast and blurred internal structures. The embryo information presented by such imaging results is very limited, which will seriously affect the accuracy of embryo morphology assessment.

[0003] Existing technologies include optical devices that reduce the focus and improve image contrast by shrinking the aperture of the aperture stop. However, since these devices can only improve contrast and cannot create a three-dimensional relief effect, the internal spatial layers of the embryo are still indistinguishable, meaning that the internal structure remains blurry. Furthermore, shrinking the aperture leads to a decrease in light flux accumulation, thus requiring a significant increase in exposure time (usually 3-5 times longer) to achieve imaging. This not only reduces image acquisition efficiency and affects timeliness, but also may introduce noise that affects accuracy. Summary of the Invention

[0004] To address the problems existing in the prior art, this invention discloses a stereoscopic imaging optical device and a culture chamber.

[0005] A stereo imaging optical device includes a light source module, a light processing module, a sample placement stage for placing a sample, and an imaging module arranged in sequence. The light beam emitted by the light source module illuminates the sample placement stage after passing through the light processing module, and the light beam passing through the sample placement stage enters the imaging module to form an image.

[0006] The light processing module sets the angle between the light beam incident on the sample placement stage and the imaging optical axis of the sample placement stage to α, where α is 15-25°.

[0007] Specifically, the light source module can select an appropriate wavelength band according to the specific imaging object. When used for embryo imaging, a 620-650nm red LED can be selected. This wavelength band is a sensitive band that does not damage the embryo and can effectively avoid the interference of light on embryo development. In addition, the light source module can also be equipped with a condenser lens, which is installed below the light source to collect the divergent beam emitted by the light source and improve the utilization rate of the light source.

[0008] The angle between the light beam after passing through the light processing module and the imaging optical axis of the sample placement stage is α, that is, the light beam is obliquely incident into the sample placement stage (sample). The obliquely incident light beam is easy to form light and shadow differences, providing optical conditions for forming a three-dimensional relief imaging result. Having a three-dimensional relief is the most important condition for obtaining a three-dimensional image.

[0009] Preferably, the light processing module is provided with a field stop, a field lens, an aperture stop, and a focusing lens in sequence in the direction away from the light source module. The angle between the field stop and the illumination optical axis is β, where β = 90° - α, and the angle between the optical axis of the focusing lens and the imaging optical axis of the sample placement stage is α.

[0010] Specifically, the beam enters the sample placement stage at an angle α with the imaging optical axis of the sample placement stage, and the angle between the field stop and the illumination optical axis (the center line of the beam) is β. This is to control the illumination range, that is, to accurately control the size of the illumination spot on the plane where the sample placement stage is located. Effectively controlling the size of the illumination spot is a condition to ensure that the sample placed on the sample placement stage can be uniformly illuminated.

[0011] In addition, the field stop is preferably an adjustable circular stop, which can adjust the diameter of the illumination spot by adjusting the field stop.

[0012] Preferably, the aperture stop has multiple light-transmitting windows with different transmittance.

[0013] Specifically, by setting light-transmitting windows with different transmittance, the proportion of light flux in different areas of the beam can be controlled, which can avoid local overexposure or underexposure caused by beam shift. It can also provide an optical basis for improving the contrast of different areas of the sample. The difference in contrast between different areas of the sample is an important condition for the imaging results to have a three-dimensional effect.

[0014] Preferably, the light-transmitting window includes a light-blocking window and a light-transmitting window, the light-blocking window is arranged around the light-transmitting window, and there are multiple light-transmitting windows, with adjacent light-transmitting windows having different light transmittance.

[0015] Specifically, the structure of the light-blocking window surrounding the light-transmitting window can effectively block light far from the center of the beam, thereby constraining stray light.

[0016] Preferably, multiple light-transmitting windows are arranged sequentially in the direction away from the imaging optical axis of the sample placement stage, and the light transmittance of the light-transmitting windows close to the imaging optical axis of the sample placement stage is lower than that of the light-transmitting windows away from the imaging optical axis of the sample placement stage.

[0017] Specifically, in this structure, the light beam passing through the high-transmittance light-transmitting window enters the focusing lens at a larger angle, which is more conducive to obtaining a better three-dimensional relief effect in imaging.

[0018] Preferably, the field-of-view lens is a double-sided convex spherical lens with a focal length of 60-80mm; the focusing lens is a double-sided convex spherical lens with a focal length of 50-70mm.

[0019] Specifically, the field lens is used to collimate the beam into a parallel beam so that the beam can be uniformly incident into the aperture stop; the angle between the optical axis of the focusing lens and the imaging optical axis of the sample placement stage is α, so as to ensure that the beam after being adjusted by the aperture stop is still tilted and focused onto the sample placement stage at an angle of α.

[0020] Preferably, the distance between the light source module and the field stop is 10-15mm, and the distance between the focusing lens and the sample placement stage is 50-60mm; the total dimension of the light source module and the light processing module along the imaging optical axis of the sample placement stage is 250-315mm, and the total dimension of the light source module and the light processing module along the direction perpendicular to the imaging optical axis of the sample placement stage is 120-200mm.

[0021] Specifically, since the optical device provided by this invention is generally suitable for imaging small samples such as embryos, its overall structural size is small, which allows for better imaging of the sample.

[0022] A stereoscopic imaging culture chamber employs the stereoscopic imaging optical device as described in any of the preceding claims. The sample placement stage is located inside the culture chamber. The imaging module is detachably connected to the culture chamber. The light source module and the light processing module are located inside the culture chamber. A reflector is used to bend the light path to adapt to the shape and size of the culture chamber.

[0023] Specifically, the size of the culture chamber varies significantly depending on the object being cultured. For example, the embryo culture chamber is relatively small. If an optical device is to be embedded in the culture chamber, it is necessary to add a structure with two reflectors to bend the light path from the light source module to the sample placement stage.

[0024] When two reflectors are added to embed the light source module and the light processing module into the culture chamber, the sum of the height (direction of the imaging optical axis of the sample placement stage) of the light source module and the light processing module is 100-160mm, and the sum of the width is 180-200mm. The overall size is small and adaptable to the culture chamber, making it easy to embed into the culture chamber.

[0025] Compared with the prior art, the advantages of the present invention are:

[0026] The light processing module allows the light beam to enter the sample placement stage at an angle α of 15-25° with the imaging optical axis, making it easy for the beam to create light and shadow differences and providing optical conditions for the imaging result to have an embossed effect. At the same time, the angle β between the field aperture and the illumination optical axis is 90°-α. The β angle is used to control the illumination range of the beam to ensure that the sample placed on the sample placement stage is uniformly illuminated. The combination of α and β can make the imaging result have an embossed effect. In addition, the structure of multiple light-transmitting windows with different transmittances combined with the aperture aperture can make the presented result have contrast. The combination of contrast and embossed effect can make the imaging result have a good three-dimensional effect. Attached Figure Description

[0027] Figure 1 A schematic diagram of the structure of the stereo imaging optical device provided by the present invention (direct light path).

[0028] Figure 2 A schematic diagram of the structure of the stereo imaging optical device provided by the present invention (optical path reflection and folding).

[0029] Figure 3 A schematic diagram of the aperture stop of the stereo imaging optical device provided by the present invention;

[0030] Figure 4 Comparison of imaging results provided by the present invention. Detailed Implementation

[0031] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0032] like Figure 1 The stereo imaging optical device shown includes a light source module 10, a light processing module 20, a sample placement stage 40 for placing samples, and an imaging module 30 arranged in sequence. The light beam emitted by the light source module 10 illuminates the sample placement stage 40 after passing through the light processing module 20. The light beam passing through the sample placement stage 40 enters the imaging module 30 for imaging.

[0033] The light processing module 20 sets the angle between the light beam incident on the sample placement stage 40 and the imaging optical axis 51 of the sample placement stage 40 to be α, where α is 15-25°.

[0034] The light source module 10 can select an appropriate wavelength band according to the specific imaging object. When used for embryo imaging, a 620-650nm red LED can be selected. This wavelength band is a sensitive band that does not damage the embryo and can effectively avoid the interference of light on embryo development. In addition, the light source module 10 can also be equipped with a condenser lens, which is installed below the light source to collect the divergent beam emitted by the light source and improve the utilization rate of the light source.

[0035] The angle between the light beam passing through the light processing module 20 and the imaging optical axis 51 of the sample placement stage 40 is α, that is, the light beam is obliquely incident into the sample placement stage 40 (sample). The obliquely incident light beam is easy to form light and shadow differences, providing optical conditions for forming a three-dimensional relief imaging result, which is the most important condition for obtaining a three-dimensional imaging result with a three-dimensional relief.

[0036] The light processing module 20 is provided with a field stop 21, a field lens 22, an aperture stop 60, and a focusing lens 24 in sequence in the direction away from the light source module 10. The angle between the field stop 21 and the illumination optical axis is β, where β = 90° - α. The angle between the optical axis of the focusing lens 24 and the imaging optical axis 51 of the sample placement stage 40 is α.

[0037] The beam enters the sample placement stage 40 at an angle α with the imaging optical axis 51 of the sample placement stage 40. The angle between the field stop 21 and the illumination optical axis (the center line of the beam) is β. This is to control the illumination range, that is, to accurately control the size of the illumination spot on the plane where the sample placement stage 40 is located. Effectively controlling the size of the illumination spot is a condition to ensure that the sample placed on the sample placement stage 40 can be uniformly illuminated.

[0038] In addition, the field stop 21 is preferably an adjustable circular stop, and the diameter of the illumination spot can be adjusted by adjusting the field stop 21.

[0039] like Figure 3 As shown, the aperture stop 60 has multiple light-transmitting windows with different transmittance.

[0040] By setting light-transmitting windows with different transmittance, the proportion of light flux in different areas of the beam can be controlled, which can avoid local overexposure or underexposure caused by beam shift. It can also provide an optical basis for improving the contrast of different areas of the sample. The difference in contrast between different areas of the sample is an important condition for the imaging results to have a three-dimensional effect.

[0041] The light-transmitting window includes a light-blocking window 61 and a light-transmitting window 62. The light-blocking window 61 is arranged around the light-transmitting window 62. There are multiple light-transmitting windows 62, and the light transmittance of adjacent light-transmitting windows 62 is different.

[0042] The structure of the light-blocking window 61 surrounding the light-transmitting window 62 can effectively block light far from the center of the beam, thereby constraining stray light.

[0043] Multiple light-transmitting windows 62 are arranged sequentially in the direction away from the imaging optical axis 51 of the sample placement stage 40, and the light transmittance of the light-transmitting window 62 close to the imaging optical axis 51 of the sample placement stage 40 is lower than that of the light-transmitting window 62 away from the imaging optical axis 51 of the sample placement stage 40.

[0044] With this structure, the light beam passing through the high-transmittance light-transmitting window 62 enters the focusing lens 24 at a larger angle, which is more conducive to obtaining a better stereoscopic relief effect.

[0045] The field lens 22 is a double-sided convex spherical lens with a focal length of 60-80mm; the focusing lens 24 is a double-sided convex spherical lens with a focal length of 50-70mm.

[0046] The field lens 22 is used to collimate the beam into a parallel beam so that the beam can be uniformly incident into the aperture stop 60; the angle between the optical axis of the focusing lens 24 and the imaging optical axis 51 of the sample placement stage 40 is α to ensure that the beam after being adjusted by the aperture stop 60 is still tilted and focused onto the sample placement stage 40 at an angle of α.

[0047] The distance between the light source module 10 and the field stop 21 is 10-15mm, and the distance between the focusing lens 24 and the sample placement stage 40 is 50-60mm. The total dimensions of the light source module 10 and the light processing module 20 along the imaging optical axis 51 of the sample placement stage 40 are 250-315mm, and the total dimensions of the light source module 10 and the light processing module 20 along the direction perpendicular to the imaging optical axis 51 of the sample placement stage 40 are 120-200mm.

[0048] Since the optical device provided in this embodiment is generally suitable for imaging small samples such as embryos, its overall structural size is small, which allows for better imaging of the sample.

[0049] The settings of α and β, along with the structure of the aperture stop 60 including a light-blocking window 61 and multiple light-transmitting windows 62 with different transmittances, significantly enhance the stereoscopic effect of the overall imaging result compared to existing technologies. A comparison of the embryo imaging results obtained using the scheme provided in this embodiment with those obtained using existing technologies is shown below. Figure 4 As shown.

[0050] This embodiment also includes a stereoscopic imaging culture chamber, employing the aforementioned stereoscopic imaging optical device. A sample placement stage 40 is located within the culture chamber. The imaging module 30 is detachably connected to the culture chamber. The light source module 10 and the light processing module 20 are located within the culture chamber. Figure 2 As shown, the reflector 70 can be used to bend the light path to fit the shape and size of the culture chamber.

[0051] The size of the culture chamber varies significantly depending on the object being cultured. For example, the embryo culture chamber is relatively small. If an optical device is to be embedded in the culture chamber, it is necessary to add a structure with two reflectors 70 to bend the light path from the light source module 10 to the sample placement stage 40.

[0052] When two reflectors 70 are added to embed the light source module 10 and the light processing module 20 into the culture chamber, the sum of the heights of the light source module 10 and the light processing module 20 (in the direction of the imaging optical axis 51 of the sample placement stage 40) is 100-160mm, and the sum of their widths is 180-200mm. The overall size is relatively small and fits the culture chamber, making it easy to embed into the culture chamber.

Claims

1. A stereoscopic imaging optical device, comprising a light source module, a light processing module, a sample placement stage for placing a sample, and an imaging module arranged sequentially, characterized in that, The light beam emitted by the light source module illuminates the sample placement stage after passing through the light processing module, and the light beam passing through the sample placement stage enters the imaging module to form an image. The light processing module sets the angle between the light beam incident on the sample placement stage and the imaging optical axis of the sample placement stage to α, where α is 15-25°.

2. The stereoscopic imaging optical device according to claim 1, characterized in that, The light processing module is provided with a field stop, a field lens, an aperture stop, and a focusing lens in sequence in the direction away from the light source module. The angle between the field stop and the illumination optical axis is β, where β = 90° - α. The angle between the optical axis of the focusing lens and the imaging optical axis of the sample placement stage is α.

3. The stereoscopic imaging optical device according to claim 2, characterized in that, The aperture stop is provided with multiple light-transmitting windows with different transmittance.

4. The stereoscopic imaging optical device according to claim 3, characterized in that, The light-transmitting window includes a light-blocking window and a light-transmitting window. The light-blocking window is arranged around the light-transmitting window. There are multiple light-transmitting windows, and adjacent light-transmitting windows have different light transmittance.

5. In the stereoscopic imaging optical device according to claim 4, a plurality of light-transmitting windows are arranged sequentially in the direction away from the imaging optical axis of the sample placement stage, and the light transmittance of the light-transmitting window close to the imaging optical axis of the sample placement stage is lower than the light transmittance of the light-transmitting window away from the imaging optical axis of the sample placement stage.

6. The stereoscopic imaging optical device according to claim 2, characterized in that, The field-of-view lens is a double-sided convex spherical lens with a focal length of 60-80mm.

7. The stereoscopic imaging optical device according to claim 2, characterized in that, The focusing lens is a double-sided convex spherical lens with a focal length of 50-70mm.

8. The stereoscopic imaging optical device according to claim 2, characterized in that, The distance between the light source module and the field stop is 10-15mm, and the distance between the focusing lens and the sample placement stage is 50-60mm.

9. The stereoscopic imaging optical device according to claim 1, characterized in that, The total dimensions of the light source module and the light processing module along the imaging optical axis of the sample placement stage are 250-315 mm, and the total dimensions of the light source module and the light processing module along the direction perpendicular to the imaging optical axis of the sample placement stage are 120-200 mm.

10. A three-dimensional imaging culture chamber, characterized in that, The stereoscopic imaging optical device as described in any one of claims 1-9 is used, wherein the sample placement stage is located inside the culture chamber, the imaging module is detachably connected to the culture chamber, the light source module and the light processing module are located inside the culture chamber, and the light path is bent by a reflector to adapt to the shape and size of the culture chamber.

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