115 results about "Cell aggregation" patented technology

The present invention relates to a microfluidic device (1) for forming a cell aggregate comprising at least one first cell (C1) and one second cell (C2), and for individually processing selected cells of the cell aggregate: - Microfluidic channel (10); - At least one main inlet (11) for fluids containing a first cell, a second cell, and a third cell, respectively, located in the first portion (101) of the microfluidic channel; - An outlet (12) located in the second portion (102) of the microfluidic channel for controlling the flow rate of fluid within the microfluidic channel; - A first auxiliary inlet (131) for at least one first auxiliary fluid, located in the first portion (101) of the microfluidic channel upstream or downstream of the main inlet (11); - At least one cell trapping section (14) positioned between the first and second parts within the microfluidic channel; - At least one first valve to control the flow rate of the first auxiliary fluid, causing the fluid containing the first cells and the second cells to flow at a predetermined height within the microfluidic channel, thereby guiding the first cells and the second cells to the first and second capture units, respectively. Equipped with, The microfluidic device relates to a microfluidic device in which each cell capture section (14) comprises at least one first capture section (141) and one second capture section (142), each first and second capture section being sized to accommodate a first or second cell, the first and second capture sections being adjacent to each other in a direction perpendicular to the bottom (100) of the microfluidic channel, and forming a cell aggregate containing the captured first and second cells, each cell being at a different height relative to the bottom of the microfluidic channel.

The present disclosure relates to methods of determining percentage viability of a cell composition comprising cell aggregates. Such methods may include providing a first aliquot of cells and a second aliquot of cells, wherein the first aliquot of cells and the second aliquot of cells have about the same concentration of cells, measuring a total number of live cells in the first aliquot of cells; measuring a total number of cells in the second aliquot of cells, and determining the percentage viability of the cell composition as a ratio between the total number of live cells in the first aliquot of cells and the total number cells in the second aliquot of cells.

The present invention aims to provide a method for efficiently producing a cell mass containing pituitary tissue from pluripotent stem cells. A method for producing a cell mass containing pituitary tissue, including the following steps (1) and (2):(1) a first step of suspension-culturing pluripotent stem cells to form a cell aggregate in the presence of a Wnt signal transduction pathway inhibiting substance,(2) a second step of suspension-culturing the aggregate obtained in the first step in the presence of a BMP signal transduction pathway activating substance and a Sonic hedgehog signal transduction pathway activating substance, thereby obtaining a cell mass comprising pituitary tissue.

This invention provides a method for propagating pluripotent stem cells (PSCs) by suspension culture in a bioreactor. [Solution] The method comprises the following steps: (i) adding a ROCK inhibitor (ROCKi) to pluripotent stem cells cultured in suspension in a bioreactor; (ii) adding a cell dissociation agent, thereby dissociating aggregates of pluripotent stem cells; (iii) diluting the cell dissociation agent added in step (ii) by adding a sufficient excess volume of culture medium to reduce the concentration of the cell dissociation agent to a concentration at which cell aggregates can be re-formed; and (iv) culturing the mixture obtained in step (iii) under appropriate conditions that allow for the proliferation of PSCs.

In a bioprinting system and method, a bioprinter may include one or more reservoirs, a printhead, and a print surface on which the bioprinting is dispensed. An optical system may collect imaging data of a plurality of cells within a cross-linkable material during and / or after passage of the plurality of cells through the bioprinting system. A computing system may generate, via a machine learning algorithm executing on the image data, a plurality of segmented regions in the image data, wherein each segmented region in the plurality of segmented region defines a boundary around a different set of cell aggregates in the plurality of cells, identify a first segmented region in the plurality of segmented regions that defines a boundary around at least a visible portion of a first cell aggregate, wherein the first cell aggregate includes an occluded portion, determine, based on an estimated shape of the first cell aggregate, an extrapolated boundary around at least some of the visible portion of the first cell aggregate and at least some of the occluded portion of the first cell aggregate, compute a quantity of cells within the first cell aggregate based on an area surrounded by the extrapolated boundary, and determine one or more parameters associated with the plurality of cells, the material flow, or the bioprinting system based on the quantity of cells.

The present disclosure relates to a method of generating pancreatic lineage cells, e.g. islet-like cell aggregates comprising pancreatic beta cells, comprising the steps of providing a single cell suspension of a population of endocrine progenitor cells (EP), allowing the EP cells in the single cell suspension to form 3D structures, and culturing the cells under conditions allowing differentiation into pancreatic mono-hormonal beta cells. The present disclosure also relates to islet-like cell aggregates obtainable by said method and their medical use.

The present invention relates to novel cell culture and transfection methods using a wildtype Caf1 ("Caf1-WT") polymer or a variant thereof. Use of a Caf1-WT polymer or a variant thereof to increase cell viability, cell proliferation, cell migration and / or cell clustering; promote the formation of a 3D cell structure; and / or promote suspension cell culture of cells that are normally adherent are also provided herein.

To provide a method for evaluating the quality of neuroretina for transplantation and neuroretina sheets for transplantation selected by said method. [Solution] The present invention provides a method for evaluating the quality of neuroretina for transplantation, comprising: extracting a part or all of a cell aggregate containing a neuroretina having an epithelial structure derived from pluripotent stem cells as a quality evaluation sample; detecting the expression of neuroretinal cell-related genes and non-neuroretinal cell-related genes in the quality evaluation sample; and determining, if the expression of neuroretinal cell-related genes is observed and the expression of non-neuroretinal cell-related genes is not observed, that (1) a neuroretina (neuroretina for transplantation) from the same cell aggregate as the cell aggregate containing a part of the quality evaluation sample, (2) a neuroretina (neuroretina for transplantation) from the same lot as the cell aggregate containing a part of the quality evaluation sample, or (3) a neuroretina (neuroretina for transplantation) from the same lot as the cell aggregate of the entire quality evaluation sample, wherein the non-neuroretinal cell-related genes include one or more genes selected from the group consisting of brain and spinal cord tissue marker genes and eyeball-related tissue marker genes.

The invention discloses a magnetic suspension three-dimensional cell culture method which comprises the following steps: performing pancreatin digestion, centrifugation and resuspension on cells, inoculating the cells into a pore plate, adding a magnetic nanoparticle solution, performing co-incubation, performing pancreatin digestion, stopping digestion, centrifuging to remove residual magnetic nanoparticles in a culture medium, and adding the culture medium for resuspension; placing a magnetic plate above the pore plate, and carrying out three-dimensional culture in an incubator; a magnet is loaded on the magnetic plate, and one side loaded with the magnet is attached to the pore plate, so that the magnet is always located above the culture medium. According to the invention, the three-dimensional magnetic suspension culture of cells is realized by preparing ferroferric oxide magnetic nanoparticles Fe3O4 (at) DA-PEG (at) PLL for enhancing cell adhesion and designing a three-dimensional culture device for large-scale magnetic suspension cell culture, and the homogenized cell aggregate with a three-dimensional structure is constructed.

In a bio-printing system and method, a bio-printer may include one or more reservoirs, a printhead, and a printing surface onto which bio-prints are dispensed. An optical system may collect imaging data of a plurality of cells within a crosslinkable material before, during, and / or after the plurality of cells pass through the bioprinting system. A computing system may process the imaging data to determine a number, distribution, concentration, and / or morphology of the plurality of cells. The machine learning system compares characteristics of the plurality of cells before, during and / or after the plurality of cells pass through the bioprinting system to a user established cellular morphology corresponding to physical characteristics of cells and / or cell aggregates within a predetermined tolerance. Additionally or alternatively, the machine learning system compares characteristics of a plurality of cells at one stage in the bioprinting system to characteristics of a plurality of cells at another stage in the bioprinting system.

A liquid treatment device is provided that shears a biological substance including cell aggregates (e.g., organoids) and filters out fragments of cell aggregates (or other biological substances) of a selected cutoff size. Further, methods of shearing a biological substance and screening out fragments of the biological substance of a selected cutoff value with a liquid treatment device are provided, including without contacting the device with another solid object.

There was a demand for a culture method that would allow for the large-scale culture of cells requiring three-dimensional culture, such as organoids and gastroids, using simple equipment. [Solution] An initial culture step in which target cells and culture medium are placed in a cylindrical culture vessel, and culture is performed using a horizontal rotation culture method in which the culture vessel is rotated with its side facing downwards in the direction of gravity and the length of the cylinder as the central axis, An induction culture step in which the culture medium is replaced with a culture medium containing an organoid inducer and cultured using the horizontal rotation culture method, The three-dimensional culture method for organoids, characterized by having a culture step in which the culture medium is replaced with a culture medium containing polysaccharides but not containing the organoid inducer, and cultured using the horizontal rotation culture method, allows for the large-scale culture of organoids without the cell aggregate adhering to any wall of the culture vessel, even when it grows large.

The invention belongs to the technical field of biomedical engineering and microfluidics, and particularly discloses a circular chamber organ chip for efficient tumor sphere culture, the circular chamber organ chip comprises a runner layer and a bottom layer which are stacked, and the lower surface of the runner layer and the bottom layer are sealed through bonding; a culture solution channel is arranged on the lower surface of the runner layer, snakelike pipelines are symmetrically arranged on the culture solution channel, and a first liquid injection channel and a second liquid injection channel are arranged at the two ends of the culture solution channel respectively; a cell culture chamber communicated with the culture solution channel is arranged on the lower surface of the runner layer; according to the cell culture chamber disclosed by the invention, by adopting a circular tissue chamber structure, the aggregation behavior of cells in a three-dimensional matrix can be obviously optimized, the cell wall attachment tendency and the asymmetric wall attachment probability are obviously reduced, and a plurality of tumor cells can be promoted to quickly and spontaneously form a sphere-like structure in a chip; the probability that the tumor balls are irregular in shape and uneven in growth is reduced, the tumor balls can be conveniently cultured, and a standardized and repeatable three-dimensional tumor model can be established.

The invention provides a whole-brain organoid based on human induced pluripotent stem cells as well as a construction method and application thereof, and belongs to the technical field of organoid construction. The method comprises the following steps: culturing human induced pluripotent stem cells in a complete culture medium to obtain a cell aggregate; culturing in an embryoid body forming culture medium to obtain a spherical embryoid body; culturing in a nerve induction culture medium tissue to form a nerve epithelium structure; embedding the single embryoid body with the nerve epithelium structure in a Matrigel gel drop, culturing in a nerve expansion culture medium to form a nerve epithelium-like structure, and replacing a mature culture medium for culturing to obtain the whole-brain organ. According to the method, by dynamically and accurately regulating and controlling culture parameters, the control ability of a cell differentiation area is improved, and interference of non-target cell types is reduced, so that the efficiency and consistency of embryoid formation and neural differentiation are directly improved, and finally, the obtained mature organoid has a more complete epithelial structure and cell polarity.

The present invention provides a method for producing regenerated hair follicle primordium that allows production of regenerated hair follicle primordium more easily and in larger amounts compared to conventional production methods.A method for producing regenerated hair follicle primordium comprising a step of obtaining hair follicle primordium by culturing a first population of cells comprising epithelial cells and a second population of cells comprising mesenchymal cells while allowing them to be in contact is provided. The production method of the present invention is characterized in that at least either one of said first population of cells and said second population of cells has formed cell aggregates before said contact, and a culture support is not used when contacting the other population of cells with said cell aggregate.

The application relates to the technical field of medical image processing, in particular to a blood smear evaluation method, device and system based on medical examination. The method adopts a traversal scanning mode to obtain blood cell images of all regions of a blood smear, and constructs an image set in column units. By screening a red blood cell central region and determining a first blood cell region and a second blood cell region, analysis is jointly performed, so that the cell aggregation degree of each blood cell image can be accurately and effectively quantified. A cell aggregation degree sequence is constructed, and uniformity in the sequence is analyzed, so that the observation difficulty of each image set is obtained, an observable region can be screened, and accurate blood smear quality evaluation is realized. The application effectively quantifies the cell aggregation degree in the image through the image acquisition mode of traversal scanning, divides a correct observable region through the special morphology of the blood smear, and then realizes accurate blood smear quality evaluation.

The application discloses a dispersion mixed solution system capable of reducing cell aggregation in a domestication process and a preparation method thereof. The system comprises 50x concentrated A liquid (100-250 g / L trypsin+12.5 g / L EDTA) and 100x concentrated B liquid (100 g / L P188+10 g / L DS), and realizes long-term stable storage and rapid preparation in the form of concentrated stock solution. When used, the concentrated stock solution is diluted and mixed in proportion, and through the synergistic effect of mild cell lysis and weakening of cell aggregation force, the cell aggregation in the domestication process is efficiently dispersed, and the cell activity and function are maintained. The preparation method is simple, the storage period is long, the dispersion effect is good, and the cell damage is small, and the application provides an effective technical means for cell domestication.

This invention provides a method for efficiently manufacturing three-dimensional structures using food ink (or cell aggregate ink) containing impurities such as granular or fibrous fragments, and also provides food three-dimensional structures or cell aggregate structures manufactured by these methods. [Solution] Multiple frames prepared for molding can be fixed to each other by inserting fixing rods or the like into common positioning holes provided in the frames. Food ink is dispensed into each filling area within the frames, spread and filled by a spreading means, and the surface is smoothed. Then the upper frames are stacked sequentially. These operations are repeated to form a composite. After the composite is fixed, the frames can be removed to obtain a three-dimensional structure (M). M can be used as is, decorated with a food 3D printer, or heated in a microwave or steamed to make it suitable for eating.

The present invention provides a method for producing regenerated hair follicle primordium that allows production of regenerated hair follicle primordium more easily and in larger amounts compared to conventional production methods.A method for producing regenerated hair follicle primordium comprising a step of obtaining hair follicle primordium by culturing a first population of cells comprising epithelial cells and a second population of cells comprising mesenchymal cells while allowing them to be in contact is provided. The production method of the present invention is characterized in that at least either one of said first population of cells and said second population of cells has formed cell aggregates before said contact, and a culture support is not used when contacting the other population of cells with said cell aggregate.

A gellable material for encapsulating a cellular aggregate in a well plate is provided. The cellular aggregate, before or after encapsulation, can be organoid or a spheroid, and typically, the cellular aggregate, once encapsulated, has substantially no movement during media exchange, handling of the well plate or tilting of the well plate. Methods for producing encapsulated cellular aggregates, and uses thereof are also provided.

The present invention provides a method for producing regenerated hair follicle primordium that allows production of regenerated hair follicle primordium more easily and in larger amounts compared to conventional production methods.A method for producing regenerated hair follicle primordium comprising a step of obtaining hair follicle primordium by culturing a first population of cells comprising epithelial cells and a second population of cells comprising mesenchymal cells while allowing them to be in contact is provided. The production method of the present invention is characterized in that at least either one of said first population of cells and said second population of cells has formed cell aggregates before said contact, and a culture support is not used when contacting the other population of cells with said cell aggregate.

The present invention relates to the field of biotechnology, and more particularly to methods for characterizing, typing, and identifying cells and their applications. The physical information of cells includes the mechanical force and / or stiffness of cells obtained in at least one of the following cases: interactions between cells and / or multicellular aggregates, cells and / or multicellular aggregates at different growth stages, different regions within multicellular aggregates, the effects of substances on cells and / or multicellular aggregates, and the effects of other physical, biological, or chemical factors on cells and / or multicellular aggregates. The present invention performs typing and identification of cells and / or multicellular aggregates by the above-described characterization method. The present invention can characterize the real-time and continuous state of cells and / or multicellular aggregates by the physical information of cells, and can identify each type and state of cells and / or multicellular aggregates in a short time, at low cost, and with high throughput, with an accuracy of 98% or more. The present invention is further limited to being realized by a characterization system, and the above effects can be achieved.

Disclosed herein are methods of analyzing a tissue sample using quantitative phase contrast microscopy, as well as corresponding microfluidic systems and corresponding devices. The methods include providing the tissue sample in a sample volume of a microfluidic system, wherein the tissue sample includes a plurality of biological cells forming a continuous tissue material. At least a portion of the tissue sample is lysed into single cells and / or cell aggregates in a carrier fluid in the sample volume. A carrier fluid flow is generated from the sample volume to a measurement volume of the microfluidic system, and a first phase shift image of the single cells and / or cell aggregates in the measurement volume is acquired with a quantitative phase contrast microscope.

The method for producing a cell aggregate including glial progenitor cells according to the present invention comprises:(1) a step of subjecting pluripotent stem cells to suspension culture in an embryoid-body-forming culture medium containing one or more SMAD signaling inhibitors and one or more Wnt signaling activators in the absence of feeder cells for 5 days to 10 days, to form a cell aggregate;(2) a step of subjecting the cell aggregate obtained in (1) to suspension culture in an embryoid-body-forming culture medium containing retinoic acid;(3) a step of subjecting the cell aggregate obtained in (2) to suspension culture in an embryoid-body-forming culture medium or neuron-and-glia-proliferating culture medium containing retinoic acid and one or more SHH signaling activators; and(4) a step of subjecting the cell aggregate obtained in (3) to suspension culture in a neuron-and-glia-proliferating culture medium containing no retinoic acid and one or more SHH signaling activators.

In a method for implantation of a physically stabilized aggregate of living cells or tissue fragment is injected into a channel provided in soft tissue filled with an aqueous gel. Also discloses are methods of stabilizing such aggregates and fragments and of forming such channel in soft tissue as well as means for carrying out the methods.

The invention relates to the technical field of human induced pluripotent stem cells, and discloses a method and a system for constructing vascular organs based on human induced pluripotent stem cells, and the method comprises the following steps: inducing human induced pluripotent stem cells to aggregate into cell aggregates, and identifying aggregate characteristics of the cell aggregates; determining mesoderm induction molecules of the cell aggregate to construct a mesoderm induction environment of the cell aggregate, and performing induced differentiation on the human induced pluripotent stem cells in the cell aggregate; detecting the expression level of the mesoderm marker gene in the mesoderm cells, constructing a vascular induction culture medium of the mesoderm cells, and inducing the mesoderm cells to differentiate into vascular lineage cells; constructing a three-dimensional extracellular matrix of the vascular lineage cells to differentiate the vascular lineage cells into a vascular network; and calculating the permeability and branch density of the vascular network to determine the fluid shear force and pulse frequency of the vascular network, and culturing the vascular network into a vascular organ. The construction stability and functionality of the vascular organoid can be improved.

Featured is a device for aggregating populations of cells that includes one or more plates that include a microwell film composite. The microwell film composite includes a first layer with a plurality of microwells and a second layer that includes a permeable body that includes a plurality of pores. The device further includes one or more ports for fluid input and / or output. Also featured are methods of making aggregates including a first population of cells (e.g., parenchymal cells (e.g., hepatocytes)) and a second population of cells (e.g., stromal cells).

The invention provides a blood sample analyzer and a blood sample analysis method.The temperature of a temperature control assembly of the blood sample analyzer reaches a first preset threshold value, to-be-detected sample liquid in the temperature control assembly is pushed into a sheath flow assembly, and the sheath flow assembly is used for obtaining pulse signals when the to-be-detected sample liquid passes through a detection area of the sheath flow assembly; the processor is used for calculating the counting stability of the to-be-detected sample liquid based on the pulse signal and obtaining a detection result of the to-be-detected sample liquid based on the counting stability. Through the mode, the to-be-detected sample liquid of the temperature control assembly is pushed into the sheath flow assembly, and the heating temperature and the heating time of the to-be-detected sample liquid are gradually increased, so that the to-be-detected sample liquid can be gradually depolymerized at the preset temperature when cell aggregation occurs; furthermore, the processor can judge whether the to-be-detected sample liquid is subjected to cell aggregation or not based on the counting stability of the to-be-detected sample liquid and obtain a corresponding detection result, so that the judgment precision of the blood sample analyzer on the cell aggregation is improved, and the counting accuracy of the blood sample analyzer is further improved.

The present application relates to cell biology, and more specifically, to a multimodal biophysical characterization apparatus, a multimodal biophysical characterization system, a multimodal biophysical characterization method, and its use. The present application can achieve multimodal, high-resolution, high-throughput, high-sensitivity, low-cost, low-damage, and configurable physical characterization of single cells or multicellular aggregates, and can subject the sample to be tested to stimuli of different types and intensities (such as mechanical stimulation, electrical stimulation, light stimulation, etc.) to better simulate the in vivo microenvironment, as well as perform operations on samples at specific positions or regions (such as marking, fixing, ablating, cutting, extracting, sorting, etc.), and combine with other characterization methods (such as protein staining, histochemical staining, single-cell sequencing, etc.) for comparative analysis. The multimodal biophysical characterization apparatus provided by the present application is suitable for fields such as synthetic biology, diagnostics, drug discovery, early tumor screening, cell therapy, and precision medicine.

This disclosure describes a computing module and method for identifying cellular aggregates in real-time based on digital holographic images that are captured using digital holographic microscopy (DHM) techniques. In particular, the computing module is configured to use artificial intelligence models and data analysis techniques to analyze the captured DHM images. The analyzed DHM images are then classified and quantified to identify cellular patterns which are then statistically analyzed to reveal patterns linking the cellular patterns to associated diseases.