84 results about "Embryogenesis" patented technology

The present invention provides methods and compositions for the identification of transgenic seeds. This is accomplished by use of screenable markers linked to aleurone-specific promoters. The screenable markers can be provided as gene fusions with selectable markers, allowing both selection and screening of transformants. The use of aleurone-specific promoters, which also direct expression in embryogenic tissues, allows efficient selection of transgenic cells and the screening of viable transgenic seeds, while avoiding the deleterious effects associated with constitutive expression of screenable marker genes. Screening of transgenic seeds avoids the need for growing and assaying of seeds for transgenes and allows implementation of automated seed screening techniques for the identification of transgenic seeds.

The invention relates to transformed, embryogenic microspores and progeny thereof characterized by being transformed by Agrobacterium tumefaciens, capable of leading to non-chimeric transformed haploid or doubled haploid embryos that develop into fertile homozygous plants within one generation and containing stably integrated into their genome a foreign DNA, said DNA being characterized in that it comprises at least one gene of interest and at least base pairs within the right border sequence of Agrobacterium T-DNA. The invention furthermore relates to a method for the incorporation of foreign DNA into chromosomes of microspores comprising the following steps: a) infecting of embryogenic microspores with Agrobacteria, which contain plasmid carrying a gene of interest under regulatory control of initiation and termination regions bordered by at least one T-DNA border, b) Washing out and killing the Agrobacteria after co-cultivation.

Methods are provided for transforming freshly isolated, immature maize embryos and for producing transgenic maize plants. The methods comprise obtaining immature embryos from a maize plant, contacting the embryos with an auxin-depleted or phytohormone-depleted transformation support medium and introducing a nucleotide construct into cells from the embryos prior to subjecting the embryos to conditions which promote embryogenic-tissue formation. The methods additionally comprise identifying or selecting transformed cells and regenerating such cells into transformed maize plants.

Protoplasts which regenerate reproducibly in a short time to normal, fertile plants can be regenerated from an auxin-autotrophic genotype of Zea mays (L.). Starting from immature embryos on hormone-free media, an auxin-autotrophic, embryogenic callus is formed on the shoot basis of the seedlings, which callus retains its embryogenic potential over a substantial period of time when subcultured on hormone-free medium. In addition to fully-developed embryos, adventitious embryos are also formed under suitable culture conditions (6-9% of sucrose in the medium). When the sucrose content is reduced to 2-3% and 2,4-dichlorophenoxyacetic acid is added, soft, granular calli are formed which consist of embryogenic cell aggregates (type II callus). After subculturing the type II callus in the form of a cell suspension culture, totipotent protoplasts can be isolated. From these protoplasts, the maize plants according to the invention are regenerated.

The invention relates to a tissue culture propagating method of Manihot esculenta Crantz. As one of the global three-top tuber crops and the number one alimentarn crop in Africa, the Manihot esculenta Crantz is currently ranked as a vital biological energy crop in our country. Stem section vegetative cottage is commonly adopted for propagation, and the low propagation rate thereof is a restrictive factor for popularizing the new variety and shortening the breeding period. The method of the invention comprises the following steps: the Manihot esculenta Crantz stem section with stem apexes or lateral buds is taken as an explant, and the Manihot esculenta Crantz tissue culture plantlets are quickly obtained in virtue of the micro-propagation method; young leaves, stem apexes and axillary buds of the sterile plantlets obtained from micro-propagation are taken as explants, somatic embryo generation and plant regeneration are induced, thus the Manihot esculenta Crantz somatic cell regeneration system is established. By adopting the method, the Manihot esculenta Crantz has the advantages of high propagation rate, no genotype dependence, and the like; the method has high value on the quick propagation and large-scale production of the Manihot esculenta Crantz improved variety in a short term, and establishes a technical basis for the Manihot esculenta Crantz transgene and other breeding.

Disclosed are processes for producing stably transformed fertile wheat a system of transforming wheat via Agrobacterium. This invention provides methods transforming a variety of explants, such as freshly isolated or pre-cultured immature embryos, embryogenic callus and suspension cells. Also disclosed are methods for recovering transgenic plants after transformation within a short period of time, if the explants are regenerable at the time of transformation. Thus the frequency of somaclonal variation associated with prolonged in vitro culture period is significantly reduced. The transformation frequency using this system is comparable to or better than published methods using other systems, such as microprojectile bombardment.

A method is provided for transforming maize plants to express DNA sequences of interest from plant cell plastids. The method allows the transformation of maize plant tissue with heterologous DNA constructs by microprojectile bombardment of green light grown cultures and dark grown embryogenic cultures. The invention also provides for maize cells in which the plastids contain heterologous DNA constructs.

The invention relates to a eucalyptus urophylla*grandis embryoid induction seedling raising method and belongs to the technical field of eucalyptus tissue culture and regeneration. The eucalyptus urophylla*grandis embryoid induction seedling raising method specifically comprises the steps of: taking a eucalyptus urophylla*grandis aseptic seedling stem segment as an explant, and obtaining a complete regenerated plant through embryonic callus induction, embryoid induction and embryoid germination. The eucalyptus urophylla*grandis embryoid induction seedling raising method can be adopted for obtaining the regenerated plant by an embryogenesis way with stable results and good repeatability, can be applied in the production and large-scale commercialization of seedlings, and also lays a foundation for eucalyptus urophylla*grandis breeding.

A tissue culture technique for soybean includes using the immature soybean cotyledon as explant to induce the reproduction of somatic embryo in solid culture medium, successive transfer storage in the solid culture medium, and normally germinating to regenerate plant.

A method for inducing the embryoid of peony includes such steps as cleaning and disinfecting the explant, culturing in starting culture medium for 30-40 days, culturing in differentiating culture medium for 15-70 days to generate embryoid, separating the embryoid, culturing in culture medium of embryoid for 30-40 days, culturing in secondary culture medium for rooting, cold storage of rooted embryoid at 4 deg.C for 60-90 days, normal culturing in culture room, and transplanting.

According to the invention an embryogenic cell mass is cultured with a culture medium comprising an anti-auxin resulting in an unexpected shift in physiology from proliferation to maturation. Proliferation is reduced so that the formation of new immature embryos ceases. Reduction of proliferation facilitates the transition from proliferation to maturation and maturation frequency is increased to a much larger extend than expected. Surprisingly, it has been discovered that the quality of the somatic embryos is not reduced, although the activity of the important endogenous plant growth regulator, auxin, is reduced. As a mater of fact, the overall quality of the mature embryos harvested at the end of maturation is actually increased over the prior art.

The invention discloses a transcription factor for enhancing the embryogenesis of a plant cell and the synthesis of fatty acid as well as an encoding gene and an application thereof. The transcription factor is the following proteins: (a) a protein formed by an amino acid sequence disclosed by the sequence 3 in a sequence table; (b) relevant proteins derived from (a), enhancing the embryogenesis of the plant cell and obtained through replacing and/or losing and/or adding one or a plurality of amino acids in the amino acid sequence disclosed by the sequence 3 in the sequence table. The invention also discloses the encoding gene of the transcription factor and a recombined expression vector containing the gene, a transgene clone or a recombined germ. The transcription factor for enhancing the embryogenesis of a plant cell and the synthesis of fatty acid and the encoding gene thereof have great application values, such as enhancing the embryogenesis of the plant cell, culturing transgene plants with improved fatty acid content, being used as screening marks of the transgene plates, enabling plants to have asexual reproduction capacity and producing synthetic seed.

The invention relates to the field of genetic transformation, specifically to a wild tomato Solanum sitiens somatic embryo induction method and a somatic embryo genetic transformation method. The induction method provided by the invention comprises the following steps: (1) culturing Solanum sitiens aseptic seedlings;(2) carrying out dark culture and inducing roots, stems (without axillalry buds) and leaves of Solanum sitiens on a embryogenic callus induction medium ECIM to generate embryogenic callus;(3) transferring the generated embryogenic callus onto a somatic embryogenesis induction medium SEIM in the light to induce somatic embryogenesis; and (4) inducing budding and seedling.

The invention discloses a method for increasing the embryogenesis rate of microspores of Wucai, and belongs to the field of vegetable chemical control. The method further comprises the step of spraying brassinolide to seedlings with 6-7 main leaves before obtaining the microspores of the Wucai, the concentration of the brassinolide added to a culture medium is 0.3 mg/L, the concentration of the brassinolide sprayed to the seedlings of the Wucai is 50 mg/L, and the spraying parts of the seedlings of the Wucai are the back surfaces of the leaves until the surfaces of the leaves drip water. The method provided by the invention can effectively improve the occurrence rate of microspore embryos of the Wucai.

The invention provides an armeniaca sibirica somatic embryogenesis method, and belongs to a seedling technology in the field of plant cultivation and is a method for inducing somatic embryogenesis with mature armeniaca sibirica embryos. In the method, fully matured armeniaca sibirica seeds are adopted, mature embryos are taken as an explant material, are inoculated on callus induction media for cultivation and are induced to generate an embryonic callus, and the callus are proliferated, so as to achieve the purpose of expanding propagation; further induction is performed by the embryonic callus, so that the embryonic callus is differentiated to form proembryos in the prior period; synchronized cultivation is performed by early proembryos under a certain condition, so that the development of the mature embryos are promoted to realize synchronization; and the early proembryos synchronously cultivated are further matured, so as to form somatic embryos with normal germination conditions. The method has the advantages that a large number of armeniaca sibirica somatic embryos are obtained by adopting the method, so that excellent armeniaca sibirica varieties are proliferated rapidly, and therefore, the propagation coefficient is greatly improved, and the propagation period is shortened.

The invention discloses a method for rapidly propagating akebia trifoliata varaustralis seedlings through a somatic embryogenesis pathway. According to the method for rapidly propagating the akebia trifoliata varaustralis seedlings through the somatic embryogenesis pathway, immature akebia trifoliata varaustralis seeds are used as explants for inducing somatic embryos. The method mainly has the advantages that 1) zygotic embryos of immature seeds have higher cell totipotency, the somatic embryogenesis process is easily induced, the immature seeds are in a relatively sterile environment in pulp, and the tissue culture contamination rate is low; 2) no exogenous plant hormones need to be added in the process of inducing somatic embryogenesis initiation and subculture multiplication, and initiation induction and subculture multiplication can be carried out in an MS culture medium; and 3) through the somatic embryogenesis pathway, the somatic embryo proliferation efficiency is high, and a large number of akebia trifoliata varaustralis aseptic seedlings can be obtained in a short time; and 4) the induced somatic embryos can be developed into complete plants, are originated from single cells, and have the advantages of low chimerism rate, low mutation rate and the like.