67 results about "Liriodendron" patented technology

The invention provides a special medicine for hog house disinfection. The traditional Chinese medicine composition for hog house disinfection and disease prevention has the advantages of convenience in availability of materials, no chemical injury, low cost and free of worry of meat commodity inspection. The medicine for hog house disinfection, provided by the invention, comprises cortex meliae, camphor tree leaves, myrrh, senecio scandens, lygodium japonicum, solidago decurrens, mastic, honeysuckle, mint, citrus peel, moutan bark, liriodendron bark, dandelion, denseflower indigo leaf, common carpesium fruit and calamus leaves.

The invention discloses a hybrid liriodendron somatic embryogenesis synchronization control method. In the method, 2,4-D concentration is reduced gradually by utilizing a growth hormone regulating mechanism; kinetin (KT) with the dose of 0.5 mg.L<-1> is added to induce uniform embryonic cell masses; the embryonic cell masses are subjected to fractional culture by a mechanically sieving method; and different subsequent synchronization control methods are adopted according to different cell mass sizes and somatic embryogenesis modes. After the synchronization control, the somatic growth synchronization ratio of hybrid liriodendron subjected to classified screening at the stage of globular embryo reaches about 90 percent; the synchronization ratio of fractional treatment at the stages of heart-shaped embryo and torpedo-shaped embryo is about 40 percent; after a proper period of refrigeration, the synchronization ratio is increased to some extent; and the conversion rate of a mature embryo, which develops from a material at the stage of globular embryo, to a regeneration plant can reach 70 to 80 percent.

The invention relates to a method for inducing a high-frequently generating of cross-breeding liriodendron liquid suspended somatic embryo by solid-liquid alternate culture and cross-breeding liriodendron regeneration. After an immature zygotic embryo from ball-shaped stage to cotyledon stage is obtained from separating, and goes through embryonic callus induction stage, embryonic callus propagation stage, the stage of the embryonic callus tissue suspension cell system establishing and pre-multiplication, embryonic suspension cell multiplication stage, the stage of stopping embryonic suspension cell multiplication and forming of embryo, somatic embryo development stage and somatic embryo plant regenerating stage, the regenerated plant of cross-breeding liriodendron can be obtained.

The invention provides a combined bleaching agent for timber. The combined bleaching agent comprises the following main components in weight ratio per 1000 ml of solution : 15-75 g of sodium percarbonate; 5-30 g of sodium perborate; 2-8 g of sodium thiosulfate and 12-50 g of sodium sulfate. The combined bleaching agent provided by the invention has higher use security and good bleaching performance; with the adoption of the bleaching agent, dark spots, mineral lines, blue stain and the like on the timber surface and the phenomena that the timber color is uneven caused by various pollutions can be eliminated, and the color difference of heartwood can be eliminated; the overall timber color can be diluted, so that the timber surface can become white and bright and uniform in color and luster; and moreover, the original timber texture and quality can be maintained, particularly the blue-green heartwood of North American liriodendron wood can be repaired better; the bleached timber is in light yellow color, which is very attractive. The processing method further can be used for reducing the white degree reducing trend caused by phototropy, so that the light resistance performance of the timber surface can become more ideal.

The invention discloses a forestation method improving the fir wood property. The method comprises the following steps of high-quality seedling cultivation, land preparation and fertilization, tree species arrangement and planting and tending management. By conducting mixed plantation between plants or mixed plantation by rows on fir and one or more of pinus massoniana, phoebe bournei, steamed beech, liriodendron chinese and schima superba, competition between the fir and other tree species is increased, broad leaves of the phoebe bournei, the steamed beech, the schima superba, the liriodendron chinese and the like are likely to rot to form humus compared with Chinese fir leaves, multiple mineral nutrition is contained, the mineral nutrition is absorbed by the fir and then accumulated in wood of the trunk, and the wood property of the fir is improved.

The invention discloses a key gene LhHB9 for somatic embryo radicle elongation of liriodendron hybrids and application thereof. The DNAsequence of the key gene LhHB9 for somatic embryo radicle elongation of liriodendron hybrids is as shown in SEQ ID NO. 1. A cDNA sequence of the LhHB9 gene of liriodendron hybrids is cloned, an overexpression vector of the LhHB9 gene is constructed, liriodendron hybrids embryogenic callus is taken as a receptor material, and the genetic transformation is carried out by an agrobacterium-mediated method. After transgenic callus is obtained by G418 screening, it is detected and the transgene was preliminarily determined to be sucessful. Through the induction of the LhHB9 gene transgenic callus embryo, it is found that the transgenic somatic embryo radicle hasobvious elongation. The statistical data shows that the 35S:LhHB9 somatic embryo radicle is the most significant compared with the control CK, indicating that overexpression of the target gene can promote the elongation of the radicle and has good practicability.

The invention discloses liriodendron chinensis LhPIN3 genes and application of the hybrid liriodendron chinensis LhPIN3 genes. A nucleotide sequence of the hybrid liriodendron chinensis LhPIN3 genes is shown as SEQ ID NO.1. According to the hybrid liriodendron chinensis LhPIN3 genes, on the basis that a mature hybrid liriodendron chinensis somatic embryogenesis system is established, auxin is cloned to transport the LhPIN3 protein genes; through expression analysis, it is proved that the hybrid liriodendron chinensis LhPIN3 genes have larger effect on embryogenic cell development and somatic embryo growth in the process of hybrid liriodendron chinensis somatic embryogenesis; after overexpression experiments, it is found out that overexpression of the LhPIN3 can influence growth and development of plants; in addition, the expression of the LhPIN3 can save partially pin1 mutants, with functions completely lost, of the LhPIN3 genes of arabidopsis thaliana, can enable the phenotypes of the pin1 mutants to recover and enable the pin1 mutants to bloom and yield legumes; accordingly, the liriodendron chinensis LhPIN3 genes can be applied to somatic embryogenesis of the plants, adventitious root induction, adventitious bud induction and differentiation, and the like and has good application prospects.

The invention discloses a method for generating a hybrid liriodendron somatic embryo by using jasmonic acid methyl ester. The method comprises the following steps: seed sterilization, induction of embryonic callus, establishment of an embryogenic cell suspension line, acquisition and transition of liquid single cells, and induction of generation of the hybrid liriodendron somatic embryo. The method is characterized in that when generation of the hybrid liriodendron somatic embryo is induced, the jasmonic acid methyl ester and ABA are added in the culture medium, or the jasmonic acid methyl ester is independently added in the culture medium. According to the method for generating the hybrid liriodendron somatic embryo by using the jasmonic acid methyl ester, a plant growth regulator MeJa is added, the inductivity of the somatic embryo is increased effectively, the abnormal rate is reduced, the generating efficiency of the hybrid liriodendron somatic embryo is improved further, the practicability is good, and establishment of a hybrid liriodendron rapid propagation system is facilitated.

The invention relates to a method for reducing somatic embryo generating and plant regenerating by clone vegetative suspension cell line of cross-bred liriodendron. The method adopts bud, stem tip, leaf, flower organ and new branch from different parts of a mother tree as induction materials for the generating of somatic embryo and young plant is obtained after the stage of callus induction, establishing and multiplication of suspension cell line, induction of suspension cell embryo development, maturity of embryo, somatic embryo germinating and plant regenerating. The method solves the problem of the somatic embryo generating and plant regenerating can not be reduced by adult cross-bred liriodendron vegetative directly, and provides the new way with short cycle, high propagation rate and low cost.

The invention discloses a liriodendron chinensis cellulose synthase gene LcCESA1 and as well as expression protein and application thereof. The nucleotide sequence of LcCESA1 is as shown in SEQ ID NO.1; the amino acid sequence of the expression protein is as shown in SEQ ID NO.2. By taking a model plant arabidopsis as a research object, a Chinese liriodendron chinensis cellulose synthase gene LcCESA1 obtained by cloning is mediated and transformed into arabidopsis, and a T3 generation homozygosous gene plant is finally obtained by means of screening and cultivation; by observing microstructures of the wild arabidopsis and transgenetic arabidopsis, and the condition that cell walls of histocytes of vascular bundles of stem sections and the blade histocytes of the transgenetic arabidopsis are different from those of the wild arabidopsis is found, so that the LcCESA1 gene affects the cell walls of histocytes of vascular bundles of stem sections and the blade histocytes of the transgeneticarabidopsis, can promote accumulation of cellulose and has wide application.

The invention discloses a method for identifying relative relationship of liriodendron hybrid and a special kit thereof. The method comprises the following steps that (1) DNA (deoxyribonucleic acid) of leaves of the liriodendron hybrid is extracted; (2) the PCR (polymerase chain reaction) detection is performed; (3) a primer 18.2 is used for specifically amplifying a female parent of the 400bp product as Chinese liriodendron, and specifically amplifying a female parent of the 382bp product as North American liriodendron; a primer 700 is used for performing PCR amplification on the Chinese liriodendron, specifically amplifying a female parent of the 700bp product as the western seed source of the Chinese liriodendron, and specifically amplifying a female parent of the 242bp product as the eastern seed source of the Chinese liriodendron; when the PCR amplification is performed on the North American liriodendron by the primer 700, the 700bp product can be specifically obtained. The methodhas the advantages that the natural forest of liriodendron hybrid in the seedling stage can be effectively verified; the speed is quick, the efficiency is high, the result is accurate, the repeatability and stability are good, and the like.

Provided is a cutting breeding method for improving cutting rooting percentage of Liriodendron Chinese. The method is characterized by comprising the following steps: acquiring 2-5 years old thick branches and making the branches into cuttings, after standing completes, using NAA+2,4-D mixed liquor to soak 0-4 cm of cutting bases, while soaking, using an ultrasonic cell crusher to perform ultrasonic treatment on the cuttings, establishing a full-automatic mist-spraying cutting pool, paving a medium which is disinfected in the cutting pool, putting the cuttings in the medium, after cuttage, using a watering can to watering, to make the cuttings tightly contact with the medium, and performing management after cuttage.

The invention relates to a method for building a fire-prevention forest belt in a Chinese fir man-made forest. The method includes the following steps that (1) a line of strawberry trees are planted along a ridge line center line, and the two sides of a ridge line are respectively planted with two lines of schima superba, that is, four lines of schima superba are planted in total; (2) a tourism path is built in the Chinese fir man-made forest or a mountain baseline serves as the tourism path, wherein the tourism path is three meters wide, two lines of landscape tree species such as liriodendron Chinese, mangnolia officinalis, cyclobalanopsis glauca, gingko or privet and oleander which are high in fire resistance are planted from the two sides, 2.5 m away from the center line, of the tourism path till the planted landscape tree species are connected with the schima superba planted in the step (1); (3) a fire-prevention forest belt closed ring is formed, wherein after trees in the step (1) and the step (2) are planted, the periphery of one slope surface of the Chinese fir man-made forest in the mountainous area is surrounded by the schima superba forest, and the fire-prevention forest belt closed ring is formed. By means of the method, the problem that the forest belt width is difficult to control when the fire-prevention forest belt is built is solved theoretically and practically, and the situation that the fire-prevention forest belt is built relaying on experience is changed. The technological method has wide prospects in application and popularization.

The invention discloses a method for constructing a particle gun mediated liriodendron hybrids transformation system. The method comprises the following steps: 1) taking embryonic callus of liriodendron hybrids, and performing high osmotic treatment; 2) extracting plasmids, preparing gold dust, and preparing micro-particles; 3) performing particle bombardment; 4) transferring the callus to restoreto a normal level to perform dark recovery culture; 5) performing embryoid induction culture, performing light culture after embryoid grows out, and inoculating into a rooting medium to perform screening culture after seedlings grow out; and 6) performing molecular detection, thereby obtaining the liriodendron hybrids transformation system. According to the method for constructing the particle gun mediated liriodendron hybrids transformation system, disclosed by the invention, the feasibility of the particle gun mediated liriodendron hybrids system is explored, and a reliable experimental foundation is provided for transforming liriodendron hybrids by mediating target genes with a particle gun in future, so that a novel technology is created for functional gene transformation of the liriodendron hybrids, and a technical support is provided for researching the effects of various functional genes in the liriodendron hybrids.

The invention discloses a health muscle-relaxing and bone-strengthening tea wine and a preparation method thereof. The health muscle-relaxing and bone-strengthening tea wine comprises the following raw materials in parts by weight: 8-10 parts of astragalus membranaceus, 3-4 parts of radix pseudostellariae, 2-4 parts of semen strychni, 5-7 parts of radix polygonati officinalis, 3-5 parts of herba epimedii, 2-3 parts of rhzizoma drynariae, 6-8 parts of herba cistanche, 3-5 parts of frankincense preparata, 8-10 parts of semen boitae, 2-4 parts of rhizoma atractylodis, 1-3 parts of deer horn, 4-6 parts of radix rehmanniae preparata, 2-3 parts of sea-buckthorn, 1-2 parts of liriodendron root, 1-2 parts of aleuritopteris, 6-8 parts of a complex nutrient, 23-25 parts of white spirit and 5-8 parts of apple vinegar. Since the health tea wine is prepared from natural Chinese medicinal herbs, the health tea wine has sweet taste as well as health-caring effect; by adding rhzizoma drynariae, herba epimedii, astragalus membranaceus and the like, the tea wine has the effects of replenishing blood, nourishing blood, promoting blood circulation to remove blood stasis, clearing and activating the channels and collaterals, relaxing muscle and strengthening bone, tonifying Qi and promoting spleen yang and eliminating stagnation and removing swelling; the health-caring effect of the tea wine is improved and the tea wine is beneficial to the health.

The invention discloses an identification method for the types of descendant tree species of Liriodendron forest and a special kit thereof. The method comprises the following steps: 1) extracting leafDNA (Deoxyribonucleic Acid) of the descendant tree species; 2) performing SSR-PCR (Simple Sequence Repeat-Polymerase Chain Reaction) detection; 3) determining the descendant tree species, wherein thedescendant tree species which can specifically amplify a product of 112bp by electrophoresis are Liriodendron chinense (Hemsl.) Sarg., the descendant tree species which can specifically amplify a product of 124bp by electrophoresis are Liriodendron tulipifera Linn., and the descendant tree species which can specifically amplify products of 112bp and 124bp are hybrid of Liriodendron chinense and Liriodendron tulipifera. The identification method for the types of the descendant tree species of Liriodendron forests disclosed by the invention has the advantages of high repeatability and high stability, can effectively identify the types of descendant tree species of Liriodendron natural forests and artificial forests, particularly the descendant trees of Liriodendron natural forests and artificial forests at a seedling stage, and can identify whether seeds of seed orchards are Liriodendron chinense (Hemsl.). seeds, Liriodendron tulipifera seeds or seeds of hybrid of Liriodendron chinenseand Liriodendron tulipifera. The identification method has the advantages of fastness, high efficiency, accurate result and very high practicality.

The invention relates to a transplanting method of Hybrid Tree of Liriodendron in clay land. The method comprises the following steps: S1, improving the clay land, wherein one month before transplanting, soil amendments which are prepared from 5-10 parts of chitin, 40-50 parts of pine needles leaves, 1-2 parts of aggregate agent and 5-8 parts of gypsum, are laid on planting land surface; the laying thickness is 3-5 cm, and then the soil conditioners are mixed in the topsoil of the planting land and a place which is below the topsoil and the depth of which is 20-30 cm; S2, processing the Hybrid Tree of Liriodendron before transplanting, wherein the root cutting treatments are carried out for 1-2 years before the big tree transplanting; branches and leaves are pruned in the year of transplanting, and soil balls are removed after spraying enough water; S3, transplanting, wherein planting holes and auxiliary holes are dug, and the auxiliary holes are installed around the circumference of the planting holes; S4, processing after transplanting. The transplanting method improves the adaptability of the clay land for the Hybrid Tree of Liriodendron, and ensures the survival rate of the transplanting Hybrid Tree of Liriodendron.

The invention discloses a making method for artificial hybrid liriodendron seeds. The making method comprises the steps of cultivating hybrid liriodendron cotyledon embryos, making hybrid liriodendron cotyledon embryo capsules, preparing artificial hybrid liriodendron seed coats and the like. The artificial seeds made by the making method can replace natural tree seeds, so that the liriodendron foresting period is shortened; furthermore, the making method cannot be limited by seasons and can be applied to large-scale and large-batch high-efficiency production of the artificial hybrid liriodendron seeds; sufficient seedlings are supplied to large-scale planting of hybrid liriodendrons.

The invention discloses a composition capable of improving stress resistance of hybrid liriodendron body embryo seedlings and realizing a promoting effect. The composition is prepared from 5 to 10 parts of wheat germ agglutinin, 5 to 10 parts of peanut agglutinin, 5 to 10 parts of sodium lignosulphonate, 5 to 10 parts of high-dispersion silicic acid, 10 to 20 parts of sodium dodecyl sulfonate and70 to 140 parts of kaolin. According to the composition disclosed by the invention, massive experiments are conducted for screening; the wheat germ agglutinin and the peanut agglutinin of which the weight ratio is 1 to 1 are creatively adopted as main active ingredients and matched with the sodium lignosulphonate, the high-dispersion silicic acid, the sodium dodecyl sulfonate and the kaolin with optimal use amounts. Experimental results show that the composition provided by the invention has the beneficial effects that the resistance of the hybrid liriodendron body embryo seedlings to adversetemperature environments of 4 DEG C low temperature and 40 DEG C high temperature is remarkably enhanced, and the drought resistance of the hybrid liriodendron body embryo seedlings can be obviously improved. The composition disclosed by the invention has the advantages of scientificity and reasonability in component matching, greenness, environmental protection, low toxicity and important practical application value.

The invention discloses a liriodendron hybrids LhRGL1 gene and application thereof. The nucleotide sequence of the LhRGL1 gene is shown as SEQ ID NO.1, and the amino acid sequence of expression protein of the LhRGL1 gene is shown as SEQ ID NO.2. By cloning and authenticating the liriodendron hybrids LhRGL1 gene and analyzing the expression of the gene, functions of the gene are verified, it is found that an LhRGL1 transgenic plant is slow in growth and development, dwarfed and plenty in branch, and it can be seen that the gene has wide application in plant breeding.

The invention belongs to the field of plant breeding, and particularly relates to a method for increasing cutting survival rate of liriodendron sino-americanum. The method mainly comprises the following steps of selecting cutting substrates of the liriodendron sino-americanum and selecting cutting slips; selecting a formula of a rooting agent, a leaching manner, and a disinfection technique; and taking moisture preservation and temperature adjustment and control measures. According to the method disclosed by the invention, the problems that the cutting scion bases of the liriodendron sino-americanum are easy to rot and the rooting rate is low are solved, the cutting survival rate is increased to 92%, and the cost is greatly reduced.

The present invention discloses a medical pipefish mass reducing and swelling resolving tea substitute brewing potion and a preparation method thereof. The tea substitute brewing potion is prepared from the following raw materials in parts by weight: 45-55 parts of medical pipefish, 15-25 parts of radix cyathulae, 15-25 parts of radix salviae miltiorrhizae, 15-25 parts of red peony roots, 10-15 parts of radix curcumae, 10-15 parts of lotus seeds, 10-15 parts of rhizoma corydalis, 5-8 parts of semen alpiniae katsumadai, 5-8 parts of liriodendron barks, 5-8 parts of herba alyxiae sinensis, 5-8 parts of star anises, 5-8 parts of radix morindae officinalis, 3-5 parts of xylitol, and 2-4 parts of white granulated sugar. The preparation method consists of the following main steps: 1, raw material pre-treating; 2, crushing and mixing; 3, mixed extract preparing; 4, concentrating and drying; and 5, preparation preparing. The medical pipefish mass reducing and swelling resolving tea substitute brewing potion has functions of reducing mass and resolving swelling, relieving stasis and restoring menstrual flow, and benefiting qi and promoting salivation after being eaten for a long time, and has advantages of being health-care, fast in effects, pure natural, free of toxic or side effects, etc.

The invention discloses a rapid propagation method of liriodendron sino-americanum with leaves as explants. The method comprises the steps of pretreatment and disinfection of leaves of liriodendron sino-americanum with three genotypes of XY1, XY2 and T8, callus induction medium, differentiatial medium, strong seedling medium, rooting medium and transplanting of hardening-seedlings. The method provided by the invention is suitable for the rapid propagation of liriodendron sino-americanum, and has the advantages of high propagation speed, large proliferation coefficient and high survival rate oftransplanting. Experiments verify that under the optimal culture conditions, the average induction rate of the highest callus induction of XY1 genotype is 93.33%, and the average proliferation coefficient of maximum callus differentiation is 7.10; the average number of leaves per plant in maximum strong seedling culture of XY2 genotype is as high as 9.4; the average rooting rate of the highest rooting culture of T8 genotype is 56.00%, and the survival rate of final hardening-seedling transplanting is 89%.

The invention discloses a liriodendron transcription factor LcbHLH52 gene and an application thereof, and belongs to the technical field of plant genetic engineering. Sequences of liriodendron bHLH family are analyzed through a bioinformatics tool, and then the LcbHLH52 transcription factor gene is successfully cloned through sequencing and homologous comparison. An overexpression vector is constructed for the cloned LcbHLH52 transcription factor gene, and used to transform arabidopsis thaliana to obtain T1 generation of seeds. T2 generation of LcbHLH52 transgenic arabidopsis thaliana plants and wild type arabidopsis thaliana plants are processed at the low temperature of 4 DEG C for 3 days simultaneously. Phenotypic observation shows that the growth of the transgenic plants is unlikely to be affected by low-temperature stress, while the growth of the wild type plants is inhibited with wilted leaves. The result shows that the liriodendron LcbHLH52 gene can enhance the tolerance of arabidopsis thaliana plants to low-temperature stress, and has important application value in improving molecular breeding of plants against low-temperature stress.

The invention discloses a novel application of a liriodendron hybrid LhWOX1 gene, and belongs to the technical field of plant genetic engineering. The novel application of the liriodendron hybrid LhWOX1 gene provided by the invention specifically comprises the following application steps: constructing a vector of the liriodendron hybrid LhWOX1 gene; transforming the constructed vector of the liriodendron hybrid LhWOX1 gene into plants or plant cells; and performing cultivation and screening to obtain transgenic plants with curly leaves. The invention discovers that the liriodendron hybrid LhWOX1 gene can curl the plant leaves for the first time, and the overexpression can cause some plant leaves to be curled and change morphology; and leaf curling is a very useful agronomic trait for somecrops, so that the LhWOX1 gene provided by the present application can be used in preparation of plants having the curled leaves and cultivation of novel plant varieties, and has important applicationvalue.

The invention discloses a liriodendron transcription factor LcbHLH16421 gene and application thereof, and belongs to the technical field of plant genetic engineering. The liriodendron tulipifera bHLH family sequence is analyzed through a bioinformatics tool, and then the LcbHLH16421 transcription factor gene is obtained through cloning through sequencing and homologous comparison. An overexpression vector for the LcbHLH16421 gene is constructed, and arabidopsis thaliana is converted to obtain T1-generation seeds; LcbHLH16421 transgenic arabidopsis thaliana T2-generation plants and wild plants are treated at the low temperature of 4 DEG C for 3 days at the same time, phenotypic observation shows that the influence of low-temperature stress on the growth of the transgenic plants is small, the growth of the wild plants is inhibited, and leaves are withered. The result shows that the liriodendron tulipifera LcbHLH16421 gene can enhance the tolerance of an arabidopsis thaliana plant to low-temperature stress, and has important application value in molecular breeding for improving the low-temperature stress of the plant.

The invention relates to an external Chinese medicine tincture for treating psoriasis. According to the weight components, he washed the washed sheep's trotter root, goldenrod, chicken baby grass, hibiscus bark, centipede, white fresh bark, flavescens, cork, coptis, skullcap, cnidium, Kochia chinensis, psoralen, Each of Dafengzi, Clove, and Baibu are immersed in 2500 grams of 95% medical alcohol, sealed in a large mouth bottle, soaked for half a month to one month, and removed to obtain a medicinal solution. Then mix and stir with borneol, benzoic acid, salicylic acid, and the above-mentioned medicinal liquid evenly, and promptly make this traditional Chinese medicine tincture. The traditional Chinese medicine tincture can quickly kill obstinate fungi and bacteria, eliminate skin infections, is suitable for treating various types of psoriasis, and is also suitable for treating neurodermatitis, jock itch, tinea pedis, goose palm and onychomycosis. The traditional Chinese medicine tincture is external medicine, convenient to use, abundant in medicine source, simple in preparation method, low in cost, leaves no scar after being cured by medicine, has no damage to human body, no pain, no toxic and side effects.

The invention discloses an aureobasidium aureum MR-57 and application thereof, and belongs to the field of functional microorganism screening and application, the strain MR-57 is preserved in Guangdong Microbial Culture Collection Center on November 26, 2021, and the preservation number is GDMCC No: 62084. The bacterial strain MR-57 has relatively strong inhibition and antagonism effects on seven pathogenic bacteria such as column spore destroying bacteria and the like, and has relatively strong antagonism capability on liriodendron alternaria alternata, column spore destroying bacteria and mechanical bacteria echinacea; the sterile fermentation liquor of the strain MR-57 has an obvious inhibition effect on the growth of fusarium equisetum hyphae and can obviously reduce the spore germination rate of fusarium equisetum; a soil mixing method is used for verifying that the strain MR-57 has good colonization capability in soil; a potted plant biocontrol test proves that the strain MR-57 has a certain prevention and growth promoting effect on the divaricate saposhnikovia root rot, and the disease index of the divaricate saposhnikovia root rot can be obviously reduced.

The invention discloses a culture medium using gamma-aminobutyric acid to promote hybrid liriodendron somatic embryogenesis and application thereof and belongs to the technical field of plant tissue culture. The culture medium is prepared by using 5mg / L of 3 / 4MC+VC and 30g / L sucrose as the basic culture medium and adding 0-10mg / L of gamma-aminobutyric acid, wherein the added gamma-aminobutyric acid can promote hybrid liriodendron callus somatic embryogenesis, accelerate somatic embryo development and increase somatic embryogenesis efficiency. When the culture medium is applied to hybrid liriodendron somatic embryogenesis and tissue culture, high somatic embryogenesis quantity and quality and fast somatic embryogenesis can be achieved, induced somatic embryo seedling number is extremely evidently higher than that of control, lateral root number and root length are evidently higher than those of the control, each callus can averagely form 20 regenerated plants, and the culture medium isquite high in practical application value.

The invention discloses a method for somatic embryogenesis of Liriodendron chinensis by using methyl jasmonate, including seed sterilization, induction of embryogenic callus, establishment of embryogenic suspension cell lines, and acquisition and transition of liquid single cells . The step of inducing the occurrence of somatic embryos of Liriodendron chinensis, characterized in that, when inducing the somatic embryos of Liriodendron chinensis, methyl jasmonate and ABA are added to the culture medium, or methyl jasmonate is added separately. The method for utilizing methyl jasmonate of the present invention to carry out hybrid Liriodendron body rapid propagation, in the hybrid Liriodendron somatic embryogenesis process, adding plant growth regulator MeJa effectively improves somatic embryo induction rate, reduces deformity rate, and further It is very practical to improve the somatic embryogenesis efficiency of Liriodendron hybridum, and it is beneficial to the establishment of rapid propagation system of Liriodendron hybridum.