43 results about "Liriodendron chinense" patented technology

The invention discloses an air freshener. The air freshener is prepared from the following raw material components in parts by weight: 81-90 parts of total plant hydrosol and 19-21 parts of total plant volatile oil, the total plant hydrosol includes liriodendron chinense leaf sol, albizia flower sol, phoenix tree flower sol and ginkgo leaf sol, and the total plant volatile oil includes liriodendron chinense leaf volatile oil, albizia flower volatile oil, phoenix tree flower volatile oil and ginkgo leaf volatile oil. The invention further discloses a preparation method of the air freshener; theair freshener not only can remove peculiar smell, relieve the mood and refresh the mind, but also can sterilize, fight against bacteria and diminish inflammation; and the air freshener has a strong antibacterial function, can inhibit the growth and reproduction of the bacteria in air and the surface of objects, and the air freshener is safe and non-toxic, and high efficient and environmental-friendly.

The invention discloses a liriodendron hybrids LhRGL1 gene and application thereof. The nucleotide sequence of the LhRGL1 gene is shown as SEQ ID NO.1, and the amino acid sequence of expression protein of the LhRGL1 gene is shown as SEQ ID NO.2. By cloning and authenticating the liriodendron hybrids LhRGL1 gene and analyzing the expression of the gene, functions of the gene are verified, it is found that an LhRGL1 transgenic plant is slow in growth and development, dwarfed and plenty in branch, and it can be seen that the gene has wide application in plant breeding.

The invention provides a grafting method of liriodendron chinense and relates to the technical field of planting grafting. The grafting method of the liriodendron chinense disclosed by the invention comprises the following steps: preparing scions; preparing rootstock; grafting; performing final-period management of grafted seedlings. According to the grafting method of the liriodendron chinense disclosed by the invention, 1-2 years of wild liriodendron chinense are used as the scions, the scions are semi-lignified, development of the scions is full, and more nutrient substances are stored, andtherefore, the grafting method is suitable for grafting big rootstock and has the advantages of quick sprouting, early formation of tree crowns, smooth grafting healing part and high grafting survival rate.

The invention discloses a method for somatic embryogenesis of Liriodendron chinensis by using methyl jasmonate, including seed sterilization, induction of embryogenic callus, establishment of embryogenic suspension cell lines, and acquisition and transition of liquid single cells . The step of inducing the occurrence of somatic embryos of Liriodendron chinensis, characterized in that, when inducing the somatic embryos of Liriodendron chinensis, methyl jasmonate and ABA are added to the culture medium, or methyl jasmonate is added separately. The method for utilizing methyl jasmonate of the present invention to carry out hybrid Liriodendron body rapid propagation, in the hybrid Liriodendron somatic embryogenesis process, adding plant growth regulator MeJa effectively improves somatic embryo induction rate, reduces deformity rate, and further It is very practical to improve the somatic embryogenesis efficiency of Liriodendron hybridum, and it is beneficial to the establishment of rapid propagation system of Liriodendron hybridum.

The invention discloses a regeneration method adopting pure solid culture for a somatic embryo plant of liriodendron chinense. The method comprises steps as follows: 1) hybrid liriodendron chinense embryonic calli are induced; 2) 3 / 4 MS, VC with concentration of 5mg / L, saccharose with concentration of 30g / L and amino oligochitosan with concentration of 0.01-0.1mg / L are taken as basic media, and the hybrid liriodendron chinense embryonic calli are cultured in dark at 24 DEG C; 3) the material is transferred to a tissue-culture vessel in a light culture room and cultured continuously with the 3 / 4 MS basic medium until the regeneration plant is formed; 4) the regeneration plant is acclimated and transplanted. According to the regeneration method adopting pure solid culture for the somatic embryo plant of liriodendron chinense, the pure solid culture manner is adopted, the somatic embryo induction links including suspension culture and plate culture in the somatic embryo generation process are skipped, a hybrid liriodendron chinense somatic embryo generation system is optimized and enriched, and accordingly, the high-quality somatic embryo regeneration plant is obtained more efficiently and more conveniently; a stable and high-frequency hybrid liriodendron chinense somatic embryo generation system adopting pure solid media and having properties of being easy to operate and good in seedling resistance is established, and reference is provided for establishment of a more efficient genetic transformation receptor system of hybrid liriodendron chinense.

The invention belongs to the cutting propagation combination technology of forest trees, and specifically provides a cutting propagation method of Liriodendron tulipifera, and simultaneously provides a matching incision anti-corrosion rooting paste film. The present invention is also applicable to the propagation of Chinese mantle tree. The present invention solves the shortcomings of long rooting time and perishable cuttings of Liriodendron tulipifera in the prior art through the reasonable combination of cutting selection, soil cultivation configuration, antiseptic rooting paste film formula and spray moisturizing management, and provides a set of The formula and propagation method can prevent rot of cuttings and accelerate rooting, and the survival rate of repeated cuttings is stable at more than 85%.

The invention provides a combined bleaching agent for timber. The combined bleaching agent comprises the following main components in weight ratio per 1000 ml of solution : 15-75 g of sodium percarbonate; 5-30 g of sodium perborate; 2-8 g of sodium thiosulfate and 12-50 g of sodium sulfate. The combined bleaching agent provided by the invention has higher use security and good bleaching performance; with the adoption of the bleaching agent, dark spots, mineral lines, blue stain and the like on the timber surface and the phenomena that the timber color is uneven caused by various pollutions can be eliminated, and the color difference of heartwood can be eliminated; the overall timber color can be diluted, so that the timber surface can become white and bright and uniform in color and luster; and moreover, the original timber texture and quality can be maintained, particularly the blue-green heartwood of North American liriodendron wood can be repaired better; the bleached timber is in light yellow color, which is very attractive. The processing method further can be used for reducing the white degree reducing trend caused by phototropy, so that the light resistance performance of the timber surface can become more ideal.

The invention discloses a method for carrying out generation of hybridized liriodendron chinense somatic embryos by utilizing salicylic acid. The method comprises the following steps: establishing a hybridized liriodendron chinense suspension system, obtaining liquid single cells, transiting and inducing the generation of the hybridized liriodendron chinense somatic embryos; in a process of inducing the generation of the hybridized liriodendron chinense somatic embryos, adding the salicylic acid and ABA (Abscisic Acid) into a culture medium at the same time or independently adding the salicylic acid. According to the method for carrying out the generation of the hybridized liriodendron chinense somatic embryos by utilizing the salicylic acid, disclosed by the invention, a plant growth regulator SA is added in a generation process of the hybridized liriodendron chinense somatic embryos, so that the inductivity of the somatic embryos and the synchronism of development are effectively improved; benefits of breeding hybridized liriodendron chinense somatic cell engineering germchits are improved.

The present invention provides a compound botanical insecticide for mosquito larvae control, a preparation method therefor and applications thereof. The insecticide consists of a bulk drug and conventional auxiliaries, wherein the mass of the bulk drug is 40-80% of the mass of the insecticide, and the mass of the conventional auxiliaries is 20-60% of the mass of the insecticide. The bulk drug comprises the following components in weight percentages based on the total weight of the bulk drug: 2-20% of a tomato extract, 5-30% of a garlic extract, 5-30% of a Euphorbia humifusa extract, 5-10% of a ginger extract, 5-25% of a potato extract, 5-30% of a Cinnamomum camphora extract, 5-20% of a photinia extract, 5-15% of a Liriodendron chinense extract, and 4-10% of a Camptotheca acuminata extract. The compound botanical insecticide for mosquito larvae control provided by the present invention is high in efficiency, free of residuals, low in production cost, economical and practical, environmentally-friendly, and excellent in comprehensive properties, and comprises agricultural wastes.

The invention relates to an asexual propagation method of michelia compressa. The asexual propagation method includes the steps that firstly, seedlings of the michelia compressa are manufactured; secondly, pretreatment of a scion of michelia compressa is performed; thirdly, a grafting method is adopted; fourthly, management after grafting is performed. Liriodendron chinense is used as a rootstock, and the cut-grafting method is used for grafting the michelia compressa. After cutting is sequentially soaked into a plant growth regulator and 1%DMSO, the rootstock is inserted, and after the cambium of the scion and the cambium of the rootstock are aligned, an anti-drop film is used for tightly winding and wrapping a port from bottom to top. According to the asexual propagation method of the michelia compressa, a great number of root-included seedlings of the michelia compressa can be obtained, the rate of reproduction is 81-86 times, and after the seedlings are obtained, the seedlings are grafted on the Liriodendron chinense, namely the rootstock, and the survival rate is 95%. After transplantation on a field is achieved for two years, the tree vigor is strong, a tree is resistant to diseases and capable of wintering safely, the problem of vacancy of the market resource of the michelia compressa is effectively solved, and the asexual propagation method of the michelia compressa is wide in application prospect.

The invention belongs to the technical field of protection and utilization of plant resources, and in particular relates to a method for cultivating tree seedlings of the genus Liriodendron. The method for cultivating Liriodendron forest tree seedlings provided by the invention directly utilizes forest land resources to increase the nutrient supply and photosynthesis of the mother tree through thinning, pruning and fertilization, so as to improve the quality of the seeds; The fallen leaves of tree species are mixed into the soil to keep the soil loose and air-permeable, and promote seed germination; furthermore, by spreading agricultural water-retaining agent and chaff ash, the water-holding capacity and air-permeability of the soil can be increased, and the cost of irrigation can be reduced; The surface fertilizer promotes the growth of seedlings and strong roots, and increases the survival rate of transplanting. The cultivation method of Liriodendron tree seedlings provided by the invention not only has a high seedling emergence rate, but also is simple and easy to implement, saves land, has low technical difficulty, and is easy to popularize. Rapid development has a great impetus.

The invention provides a DNA barcode primer pair for amplification of liriodendron chinense(Hemsl.)Sarg. and an identification method of the liriodendron chinense(Hemsl.)Sarg. and a germplasm source of the liriodendron chinense(Hemsl.)Sarg., and belongs to the technical field of plant molecular identification. The DNA barcode primer pair for amplification of the liriodendron chinense(Hemsl.)Sarg. comprises an upstream amplification primer and a downstream amplification primer. The sequence of the upstream amplification primer is shown in SEQ ID No.1, and the sequence of the downstream amplification primer is shown in SEQ ID No.2. The identification method of the liriodendron chinense(Hemsl.)Sarg. comprises the following steps of 1, extracting a whole genome DNA of a to-be-detected sample; 2, taking the whole genome DNA as a template, and utilizing the DNA barcode primer pair for PCR amplification to obtain an amplification product; 3, sequencing the amplification product to obtain specific sequence information of the amplification product, comparing the specific sequence information with DNA barcode standard sequences of the liriodendron chinense(Hemsl.)Sarg., and then determining tree species and the regional germplasm source of the to-be-detected sample. The method is easy to operate and high in accuracy.