16 results about "Liriodendron chinense" patented technology

The invention discloses a method and a device suitable for CUAMP. The invention discloses a method for extracting liriodendron tulipifera cell nucleuses by a Tag technology and application of the liriodendron tulipifera cell nucleuses. Hybridized liriodendron tulipifera calluses are used as materials, and protoplasts are released through enzymolysis solution vacuumizing and mild enzymolysis for 1.5 h; the method comprises the following steps: sequentially purifying by using a W5 solution and mannitol, detecting the activity by FDA, cracking by using an NE buffer solution, and washing by using a Wash buffer solution in two steps, thereby obtaining 2 * 10 < 6 >-3 * 10 < 6 > high-purity and high-integrity cell nucleuses. The obtained core is clean in background and complete in membrane structure, and can be directly used for CUTamp; tag is used for building a library, so that the magnetic bead capturing efficiency is improved by 46%, and protein-DNA interaction high-throughput sequencing under the condition of low sample size is realized. The method disclosed by the invention is simple and convenient to operate and good in repeatability, and provides key technical support for epigenetic research of rare tree species such as liriodendron tulipifera.

The invention relates to the technical field of hybrid liriodendron and discloses a hybrid liriodendron topping and dwarfing device which comprises a bottom plate and a supporting rod, the supporting rod is fixedly connected to the outer wall of the bottom plate, a lifting mechanism is arranged in the supporting rod, and a stabilizing assembly used for improving the stability of the whole bottom is arranged on the side, away from the supporting rod, of the bottom plate. The outer wall of the supporting rod is slidably connected with a fixing plate. According to the method, the gathering plate I and the gathering plate II move towards the middle, the branches and leaves at the top of the tulip tree between the gathering plate I and the gathering plate II are gathered towards the middle, and the tulip tree at the gathering part can be cut and dwarfed through the movement of the cut-off knife. Branches and leaves at the top of the liriodendron tulipifera can be prevented from being cut and dwarfed many times, the branches and leaves at the top of the liriodendron tulipifera are gathered and then cut and dwarfed, the branches and leaves at the top of the liriodendron tulipifera can be cut and dwarfed at a time, and the cutting and dwarfing efficiency of the liriodendron tulipifera is improved.

The invention discloses a method for in-vitro induction of tulip tree cluster buds and regeneration seedlings. The method comprises the following steps: firstly, cutting 3-4cm of a plant material containing terminal buds or axillary buds from a liriodendron twig, disinfecting, removing plant endophyte, then cutting 0.5-1cm of the terminal buds or axillary buds as explants, then putting the explants on a cluster bud induction culture medium for induction culture for about 30 days, then cutting off the induced cluster buds, and putting the induced cluster buds on an elongation culture medium for culture; when the buds extend to be rootless seedlings with the length of 2-3 cm, the seedlings are transferred to a rooting culture medium for rooting induction, and the seedlings can root after being cultured in the rooting culture medium for about 40 days. According to the method, terminal buds and axillary buds are used as explants, growth of endophytes is effectively controlled, a cluster bud induction culture medium, a bud elongation culture medium and a rooting culture medium are screened out, an in-vitro regeneration system is established, and technical support is provided for propagation of liriodendron plants.

The present application relates to the technical fields of hybrid liriodendron, and disclose a kind of hybrid liriodendron top cutting dwarf device, including bottom plate, support rod, support rod is fixedly connected in the outer wall of bottom plate, the inside of support rod is provided with lifting mechanism, the side of bottom plate away from support rod is provided with the firm component for increasing the stability of overall bottom, the outer wall of support rod is slidably connected with fixed plate;Through the effect of moving to middle by gathering board one and gathering board two, the top branches and leaves of liriodendron in gathering board one and gathering board two middle are gathered to middle, again by the moving of cutting knife, the effect that liriodendron of gathering part can be cut off dwarf, compared with the top branches and leaves of liriodendron in prior art are scattered state top cutting, can avoid the top branches and leaves of liriodendron to be cut off dwarf multiple times, and by gathering after cutting off dwarf, the top branches and leaves of liriodendron can be cut off dwarf once, improve liriodendron cutting dwarf efficiency.

The invention discloses a method for establishing an efficient organ in-vitro regeneration system of improved hybrid liriodendron species, and belongs to the technical field of plant cell engineering. The method comprises the following steps: obtaining a hybrid liriodendron chinense improved seed somatic embryo regenerated plant which is subjected to illumination culture for 60 days, taking a stem segment or a stem tip as an explant for in-vitro bud induction, screening out a culture medium formula which is most suitable for the stem segment and the stem tip, and vertically placing the stem segment; carrying out proliferation and elongation culture on the induced in-vitro buds under specific conditions, and screening out the most suitable proliferation culture medium formula and elongation culture medium formula; carrying out rooting culture on the elongated buds, and screening out an optimal rooting culture medium formula; finally, seedling hardening and transplanting are conducted, the seedling hardening method is that bottles are sealed for 3 days, the bottles are opened for 1 day, and a transplanting matrix is prepared from humus nutrient soil and perlite according to the ratio of 7: 4. The method is high in regeneration efficiency, simple and convenient to operate and capable of meeting large-scale production requirements.

The present invention discloses a SNP molecular marker combination for identifying superior Liriodendron chinense strains and its application, belonging to the field of biological breeding technology. The SNP molecular marker for identifying superior Liriodendron chinense strains disclosed in the present invention consists of 10 SNP molecular markers, and each SNP molecular marker corresponds to a SNP site. Based on the results of Hyper‑seq high-throughput sequencing, the present invention has developed 10 pairs of SNP primers. Multiple primer combinations can be used to identify candidate superior strains and can be used to construct fingerprint maps of superior Liriodendron chinense strains. Superior Liriodendron chinense strains can be distinguished at the molecular level. This method has the advantages of high accuracy, good stability, simple operation, etc., and can play a positive role in Liriodendron chinense molecular marker-assisted breeding and variety protection.

The invention discloses a method for establishing a hybrid liriodendron somatic embryogenesis system based on an LhAHL10 / 15 gene, and relates to the technical field of plant genetic engineering. According to the method for establishing the hybrid liriodendron somatic embryogenesis system based on the LhAHL10 / 15 gene disclosed by the invention, the purpose of promoting the generation of the hybrid liriodendron somatic embryogenesis is achieved by promoting the LhAHL15 gene or inhibiting the expression of the LhAHL10 gene; the nucleotide sequences of the LhAHL15 gene and the LhAHL10 gene are respectively as shown in SEQ ID NO. 3 and SEQ ID NO. 1. The result shows that overexpression of the LhAHL15 in the hybrid liriodendron is beneficial for improving the indirect somatic embryogenesis efficiency of the hybrid liriodendron. The overexpression of the LhAHL10 in the hybrid liriodendron can also improve the somatic embryo generation efficiency, but can influence the modeling development process of somatic embryos, so that the development of the somatic embryos is stagnated in the globular embryo stage.

The invention discloses an LhNCED3b gene as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the LhNCED3b gene provided by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of protein expressed by the LhNCED3b gene is as shown in SEQ ID NO.2. According to the application, by constructing a CRISPR / Cas9 editing vector, LhNCED3b knockout of hybrid liriodendron is realized, and in combination with LhNCED3b gene overexpression analysis, it is found that under a salt stress condition, an LhNCED3b overexpression strain significantly improves salt tolerance by inhibiting active oxygen accumulation, maintaining moisture balance and enhancing membrane stability; and the knockout strain shows serious stress damage due to pore regulation disorder and oxidation resistance weakening, which indicates that the LhNCED3b gene positively regulates and controls the salt stress response of the hybridized liriodendron fine variety by regulating and controlling the redox steady state and the osmotic protection mechanism.

The invention relates to a liriodendron hybrids LhSAMDC gene and application thereof, and belongs to the technical field of gene engineering. The CDS nucleotide sequence of the LhSAMDC gene disclosed by the invention is as shown in SEQ ID NO.1, and adenosine methionine decarboxylase coded by the LhSAMDC gene plays a key role in plant polyamine metabolism. The invention also provides a primer pair (SEQ ID NO.2 and SEQ ID NO.3) for amplifying the gene and a plant expression vector containing the gene. Transgenic arabidopsis thaliana experiments show that the overexpression of the LhSAMDC gene can significantly improve the biomass, plant height, leaf SPAD value and stress resistance of the plant. The invention provides a new technical means for plant breeding, can be used for cultivating new plant varieties with stronger growth vigor, higher biomass and better stress resistance, and has a wide application prospect.

This invention discloses a promoter, ProWUS, specifically for the apical meristem of hybrid tulip tree and its applications, belonging to the field of plant genetic engineering technology. Based on the genome sequence information of Chinese tulip tree, the ProWUS promoter sequence was cloned, and its nucleotide sequence is shown in SEQ ID NO.1. Driven by the cloned ProWUS promoter, the reporter gene can be specifically expressed at the apical meristem of the plant. Using the promoter provided by this invention, the specific expression of the corresponding target gene in the apical meristem can be induced in plant genetic engineering, which not only enriches the molecular biological theoretical basis of plant shoot tips but also has important application value in the development of plant apical meristems and the maintenance of stem cells.

The invention discloses a method for efficiently inducing hybrid liriodendron somatic embryogenesis, and belongs to the technical field of plant embryogenesis. The method mainly comprises the following steps: performing callus multiplication culture on embryogenic calluses of hybrid liriodendron in a callus multiplication culture medium containing a DNA methyltransferase inhibitor 5-Aza-dC or Zebularine, then transferring the embryogenic calluses into a somatic embryo induction culture medium for somatic embryo induction, and obtaining mature hybrid liriodendron somatic embryos after somatic embryo induction is completed. The method is easy to operate, and the effects of inhibiting mass proliferation of embryonic tissues, promoting generation and development of somatic embryos and improving the somatic embryo generation efficiency can be achieved only by adding a proper amount of DNA methylated transferase inhibitor in the callus proliferation stage. The method can enhance the somatic embryo induction efficiency of the hybrid liriodendron, provides a new technical scheme for optimizing the somatic embryo generation of the hybrid liriodendron by using an epigenetic regulation means, and has important application value in the field of rapid propagation of the hybrid liriodendron.

The invention discloses a liriodendron hybrids LhARIA gene as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the liriodendron hybrids LhARIA gene provided by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the expressed protein is as shown in SEQ ID NO.2. The expression mode of the LhARIA gene under cold, drought and salt stress is analyzed through a q-PCR technology, and it is found that the gene is remarkably up-regulated under the cold stress condition. An overexpression experiment of the LhARIA gene is respectively carried out in arabidopsis thaliana and hybrid liriodendron, and a result shows that the LhARIA gene remarkably improves the cold resistance of a transgenic plant through multiple mechanisms of regulating ROS metabolism, enhancing the antioxidant enzyme activity, activating an ICE-CBF-COR signal channel and the like. The invention provides important candidate genes and theoretical support for improving the cold resistance of plants by using a genetic engineering technology in future.

The application discloses a hybrid Liriodendron chinense LhTRX-h3 gene, an expression protein thereof and application, relates to the technical field of plant genetic engineering.The hybrid Liriodendron chinense LhTRX-h3 gene disclosed by the application has a nucleotide sequence as shown in SEQ ID NO.1 and an amino acid sequence as shown in SEQ ID NO.2.The application constructs an expression vector of the hybrid Liriodendron chinense LhTRX-h3 gene, transforms the expression vector of the hybrid Liriodendron chinense LhTRX-h3 gene into hybrid Liriodendron chinense, and cultivates, screens and obtains a transgenic hybrid Liriodendron chinense plant with improved non-biological stress resistance.The hybrid Liriodendron chinense LhTRX-h3 gene disclosed by the application can promote the plant to improve non-biological stress resistance, regulate plant growth and regulate the length-width ratio of plant leaves.

The application discloses a hybrid Liriodendron hybridum somatic embryogenesis system establishment method based on LhAHL10 / 15 genes, and relates to the technical field of plant genetic engineering. The hybrid Liriodendron hybridum somatic embryogenesis system establishment method based on LhAHL10 / 15 genes disclosed by the application promotes the expression of LhAHL15 genes or inhibits the expression of LhAHL10 genes, so that the purpose of promoting the somatic embryogenesis of the hybrid Liriodendron hybridum is achieved; the nucleotide sequences of the LhAHL15 genes and the LhAHL10 genes are respectively shown in SEQ ID NO. 3 and SEQ ID NO. 1. The results show that overexpression of LhAHL15 in the hybrid Liriodendron hybridum is beneficial to improving the indirect somatic embryogenesis efficiency of the hybrid Liriodendron hybridum. Overexpression of LhAHL10 in the hybrid Liriodendron hybridum can also improve the somatic embryogenesis efficiency, but can affect the patterning development process of the somatic embryos, so that the somatic embryos are developed to stagnate at the globular embryo stage.

The present disclosure relates to the field of medicaments, and particularly to use of Liriodendron chinense (Hemsl.) Sarg, or an extract thereof in the preparation of a medicament for reducing a serum uric acid level and preventing and treating uric acid nephropathy, and belongs to the field of medicaments. In one aspect, the present disclosure provides use of Liriodendron chinense (Hemsl.) Sarg. or an extract thereof in the preparation of a medicament for reducing a serum uric acid level. In another aspect, the present disclosure further provides use of Liriodendron chinense (Hemsl.) Sarg. or an extract thereof in the preparation of a medicament for treating and / or preventing uric acid nephropathy.

The invention discloses a liriodendron LCYE gene as well as an expression protein and application thereof, and relates to the technical field of plant genetic engineering. The liriodendron LCYE gene disclosed by the invention comprises an LcLCYE gene, or / and an LtLCYE gene, or / and a variant gene LcLCYE-alpha, or / and a variant gene LtLCYE-alpha, or / and the liriodendron LCYE-alpha, or / and the liriodendron LCYE-alpha. The nucleotide sequences of the gene respectively correspond to SEQ ID NO.1, 3, 5 and 7. The results of the embodiment of the invention show that the liriodendron LCYE homologous protein not only has the traditional LCYE enzyme activity, but also has the LCYB enzyme activity for catalyzing lycopene to form gamma-carotene and beta-carotene; the C-terminal structure of the liriodendron LCYE homologous protein is necessary for the LCYE enzyme activity function of the liriodendron, but does not affect the LCYB enzyme activity function of the liriodendron.