44 results about "Liriodendron chinense" patented technology

The invention is characterized by that on the basis of choice of parents of basswood generic cross and breeding of interspecfic hybridization, the immature embryo of limited quantities got respectively from normal, reciprocal an rotation cross is engaged in induction and cultivation of embryo somatric cell, produces large quantities of embryo of somatic cell in synchronizing development, and leads to cultivate embryo of somatic cell into whole tree characteristics.

The invention relates to an asexual propagation method of michelia compressa. The asexual propagation method includes the steps that firstly, seedlings of the michelia compressa are manufactured; secondly, pretreatment of a scion of michelia compressa is performed; thirdly, a grafting method is adopted; fourthly, management after grafting is performed. Liriodendron chinense is used as a rootstock, and the cut-grafting method is used for grafting the michelia compressa. After cutting is sequentially soaked into a plant growth regulator and 1%DMSO, the rootstock is inserted, and after the cambium of the scion and the cambium of the rootstock are aligned, an anti-drop film is used for tightly winding and wrapping a port from bottom to top. According to the asexual propagation method of the michelia compressa, a great number of root-included seedlings of the michelia compressa can be obtained, the rate of reproduction is 81-86 times, and after the seedlings are obtained, the seedlings are grafted on the Liriodendron chinense, namely the rootstock, and the survival rate is 95%. After transplantation on a field is achieved for two years, the tree vigor is strong, a tree is resistant to diseases and capable of wintering safely, the problem of vacancy of the market resource of the michelia compressa is effectively solved, and the asexual propagation method of the michelia compressa is wide in application prospect.

The invention provides a combined bleaching agent for timber. The combined bleaching agent comprises the following main components in weight ratio per 1000 ml of solution : 15-75 g of sodium percarbonate; 5-30 g of sodium perborate; 2-8 g of sodium thiosulfate and 12-50 g of sodium sulfate. The combined bleaching agent provided by the invention has higher use security and good bleaching performance; with the adoption of the bleaching agent, dark spots, mineral lines, blue stain and the like on the timber surface and the phenomena that the timber color is uneven caused by various pollutions can be eliminated, and the color difference of heartwood can be eliminated; the overall timber color can be diluted, so that the timber surface can become white and bright and uniform in color and luster; and moreover, the original timber texture and quality can be maintained, particularly the blue-green heartwood of North American liriodendron wood can be repaired better; the bleached timber is in light yellow color, which is very attractive. The processing method further can be used for reducing the white degree reducing trend caused by phototropy, so that the light resistance performance of the timber surface can become more ideal.

The invention discloses a method for full-crown transplanting of a big liriodendron chinense tree at high temperature in summer and belongs to the technical field of big tree transplanting. The method includes following steps: 1), cutting off roots and rooting; 2), excavating and loading; 3), transporting and unloading; 4), trimming and performing antiseptic treatment; 5), improving soil; 6), digging a hole for planting; 7), planting; 8), supporting and maintaining; 9), preventing disease and pest. Through scientific transplanting and maintaining management, survival rate of the big liriodendron chinense tree after being transplanted is high, the big tree has many roots and exuberant branches and leaves, and surviving and healthy and strong growing are realized.

The invention discloses a key gene LhHB9 for somatic embryo radicle elongation of liriodendron hybrids and application thereof. The DNAsequence of the key gene LhHB9 for somatic embryo radicle elongation of liriodendron hybrids is as shown in SEQ ID NO. 1. A cDNA sequence of the LhHB9 gene of liriodendron hybrids is cloned, an overexpression vector of the LhHB9 gene is constructed, liriodendron hybrids embryogenic callus is taken as a receptor material, and the genetic transformation is carried out by an agrobacterium-mediated method. After transgenic callus is obtained by G418 screening, it is detected and the transgene was preliminarily determined to be sucessful. Through the induction of the LhHB9 gene transgenic callus embryo, it is found that the transgenic somatic embryo radicle hasobvious elongation. The statistical data shows that the 35S:LhHB9 somatic embryo radicle is the most significant compared with the control CK, indicating that overexpression of the target gene can promote the elongation of the radicle and has good practicability.

The invention discloses a liriodendron chinensis cellulose synthase gene LcCESA1 and as well as expression protein and application thereof. The nucleotide sequence of LcCESA1 is as shown in SEQ ID NO.1; the amino acid sequence of the expression protein is as shown in SEQ ID NO.2. By taking a model plant arabidopsis as a research object, a Chinese liriodendron chinensis cellulose synthase gene LcCESA1 obtained by cloning is mediated and transformed into arabidopsis, and a T3 generation homozygosous gene plant is finally obtained by means of screening and cultivation; by observing microstructures of the wild arabidopsis and transgenetic arabidopsis, and the condition that cell walls of histocytes of vascular bundles of stem sections and the blade histocytes of the transgenetic arabidopsis are different from those of the wild arabidopsis is found, so that the LcCESA1 gene affects the cell walls of histocytes of vascular bundles of stem sections and the blade histocytes of the transgeneticarabidopsis, can promote accumulation of cellulose and has wide application.

The invention belongs to the field of plant breeding, and particularly relates to a method for increasing the cuttage survival rate of hybrid Chinese tulip trees. The method mainly includes selection of cuttage mediums and cuttage twigs of the hybrid Chinese tulip trees, concentration matching of rooting agents and corresponding soaking time, the sterilization anti-corrosion technology, moisture preservation and temperature regulation and control measures. The defects that roots of the cuttage twigs of the hybrid Chinese tulip trees are prone to rotting and the rooting rate is low are overcome, and the cuttage survival rate is increased to 81%.

The invention discloses a method for constructing a particle gun mediated liriodendron hybrids transformation system. The method comprises the following steps: 1) taking embryonic callus of liriodendron hybrids, and performing high osmotic treatment; 2) extracting plasmids, preparing gold dust, and preparing micro-particles; 3) performing particle bombardment; 4) transferring the callus to restoreto a normal level to perform dark recovery culture; 5) performing embryoid induction culture, performing light culture after embryoid grows out, and inoculating into a rooting medium to perform screening culture after seedlings grow out; and 6) performing molecular detection, thereby obtaining the liriodendron hybrids transformation system. According to the method for constructing the particle gun mediated liriodendron hybrids transformation system, disclosed by the invention, the feasibility of the particle gun mediated liriodendron hybrids system is explored, and a reliable experimental foundation is provided for transforming liriodendron hybrids by mediating target genes with a particle gun in future, so that a novel technology is created for functional gene transformation of the liriodendron hybrids, and a technical support is provided for researching the effects of various functional genes in the liriodendron hybrids.

The invention discloses an air freshener. The air freshener is prepared from the following raw material components in parts by weight: 81-90 parts of total plant hydrosol and 19-21 parts of total plant volatile oil, the total plant hydrosol includes liriodendron chinense leaf sol, albizia flower sol, phoenix tree flower sol and ginkgo leaf sol, and the total plant volatile oil includes liriodendron chinense leaf volatile oil, albizia flower volatile oil, phoenix tree flower volatile oil and ginkgo leaf volatile oil. The invention further discloses a preparation method of the air freshener; theair freshener not only can remove peculiar smell, relieve the mood and refresh the mind, but also can sterilize, fight against bacteria and diminish inflammation; and the air freshener has a strong antibacterial function, can inhibit the growth and reproduction of the bacteria in air and the surface of objects, and the air freshener is safe and non-toxic, and high efficient and environmental-friendly.

The present invention provides a compound botanical insecticide for mosquito larvae control, a preparation method therefor and applications thereof. The insecticide consists of a bulk drug and conventional auxiliaries, wherein the mass of the bulk drug is 40-80% of the mass of the insecticide, and the mass of the conventional auxiliaries is 20-60% of the mass of the insecticide. The bulk drug comprises the following components in weight percentages based on the total weight of the bulk drug: 2-20% of a tomato extract, 5-30% of a garlic extract, 5-30% of a Euphorbia humifusa extract, 5-10% of a ginger extract, 5-25% of a potato extract, 5-30% of a Cinnamomum camphora extract, 5-20% of a photinia extract, 5-15% of a Liriodendron chinense extract, and 4-10% of a Camptotheca acuminata extract. The compound botanical insecticide for mosquito larvae control provided by the present invention is high in efficiency, free of residuals, low in production cost, economical and practical, environmentally-friendly, and excellent in comprehensive properties, and comprises agricultural wastes.

The invention discloses a preparation method for prevention and treatment of panonychus ulmi. Raw materials include Liriodendron chinense extract, ginger extract, Sapium sebiferum extract, Daphne extract, castor bean extract and Berberis extract. The prepared acaricidal agent has best effect and long pesticide effecting time, and the pesticidal rate is above 93%.

The invention discloses a method for carrying out generation of hybridized liriodendron chinense somatic embryos by utilizing salicylic acid. The method comprises the following steps: establishing a hybridized liriodendron chinense suspension system, obtaining liquid single cells, transiting and inducing the generation of the hybridized liriodendron chinense somatic embryos; in a process of inducing the generation of the hybridized liriodendron chinense somatic embryos, adding the salicylic acid and ABA (Abscisic Acid) into a culture medium at the same time or independently adding the salicylic acid. According to the method for carrying out the generation of the hybridized liriodendron chinense somatic embryos by utilizing the salicylic acid, disclosed by the invention, a plant growth regulator SA is added in a generation process of the hybridized liriodendron chinense somatic embryos, so that the inductivity of the somatic embryos and the synchronism of development are effectively improved; benefits of breeding hybridized liriodendron chinense somatic cell engineering germchits are improved.

The invention discloses an identification method for the types of descendant tree species of Liriodendron forest and a special kit thereof. The method comprises the following steps: 1) extracting leafDNA (Deoxyribonucleic Acid) of the descendant tree species; 2) performing SSR-PCR (Simple Sequence Repeat-Polymerase Chain Reaction) detection; 3) determining the descendant tree species, wherein thedescendant tree species which can specifically amplify a product of 112bp by electrophoresis are Liriodendron chinense (Hemsl.) Sarg., the descendant tree species which can specifically amplify a product of 124bp by electrophoresis are Liriodendron tulipifera Linn., and the descendant tree species which can specifically amplify products of 112bp and 124bp are hybrid of Liriodendron chinense and Liriodendron tulipifera. The identification method for the types of the descendant tree species of Liriodendron forests disclosed by the invention has the advantages of high repeatability and high stability, can effectively identify the types of descendant tree species of Liriodendron natural forests and artificial forests, particularly the descendant trees of Liriodendron natural forests and artificial forests at a seedling stage, and can identify whether seeds of seed orchards are Liriodendron chinense (Hemsl.). seeds, Liriodendron tulipifera seeds or seeds of hybrid of Liriodendron chinenseand Liriodendron tulipifera. The identification method has the advantages of fastness, high efficiency, accurate result and very high practicality.

The invention discloses a liriodendron hybrids LhRGL1 gene and application thereof. The nucleotide sequence of the LhRGL1 gene is shown as SEQ ID NO.1, and the amino acid sequence of expression protein of the LhRGL1 gene is shown as SEQ ID NO.2. By cloning and authenticating the liriodendron hybrids LhRGL1 gene and analyzing the expression of the gene, functions of the gene are verified, it is found that an LhRGL1 transgenic plant is slow in growth and development, dwarfed and plenty in branch, and it can be seen that the gene has wide application in plant breeding.

The invention discloses a liriodendron transcription factor LcbHLH52 gene and an application thereof, and belongs to the technical field of plant genetic engineering. Sequences of liriodendron bHLH family are analyzed through a bioinformatics tool, and then the LcbHLH52 transcription factor gene is successfully cloned through sequencing and homologous comparison. An overexpression vector is constructed for the cloned LcbHLH52 transcription factor gene, and used to transform arabidopsis thaliana to obtain T1 generation of seeds. T2 generation of LcbHLH52 transgenic arabidopsis thaliana plants and wild type arabidopsis thaliana plants are processed at the low temperature of 4 DEG C for 3 days simultaneously. Phenotypic observation shows that the growth of the transgenic plants is unlikely to be affected by low-temperature stress, while the growth of the wild type plants is inhibited with wilted leaves. The result shows that the liriodendron LcbHLH52 gene can enhance the tolerance of arabidopsis thaliana plants to low-temperature stress, and has important application value in improving molecular breeding of plants against low-temperature stress.

The invention discloses a novel application of a liriodendron hybrid LhWOX1 gene, and belongs to the technical field of plant genetic engineering. The novel application of the liriodendron hybrid LhWOX1 gene provided by the invention specifically comprises the following application steps: constructing a vector of the liriodendron hybrid LhWOX1 gene; transforming the constructed vector of the liriodendron hybrid LhWOX1 gene into plants or plant cells; and performing cultivation and screening to obtain transgenic plants with curly leaves. The invention discovers that the liriodendron hybrid LhWOX1 gene can curl the plant leaves for the first time, and the overexpression can cause some plant leaves to be curled and change morphology; and leaf curling is a very useful agronomic trait for somecrops, so that the LhWOX1 gene provided by the present application can be used in preparation of plants having the curled leaves and cultivation of novel plant varieties, and has important applicationvalue.

The invention discloses a hybrid liriodendron chinense cutting breeding method. A spraying slotting machine with full illumination and unblocked draining is used, the bottom layer of the slotting machine is laid with cobblestones, and gasp between the cobblestones are filled with sandstones; the middle layer is laid with river sand; the top layer is laid with a cutting matrix, and a bactericide isadded to the cutting matrix before cutting; when a cutting slip is cut, the notch in the lower end is flat, and the notch in the upper end is flat or inclined; the lower end of the cutting clip is immersed with a rooting liquid or dipped with rooting powder; a needed jack is made in the slotting machine in advance, and the lower end of the cutting clip is inserted into the jack; spraying and watering are carried out after cutting immediately, watering is carried out in small amount and frequently, it is ensured that there are water drops on leaves of the cutting clip but there is no accumulated water in the cutting matrix; and the leaves of the cutting clip are sprayed with foliar fertilizers regularly. The rooting rate and survival rate of the hybrid liriodendron chinense can be improvedgreatly, and the method also has the advantages of fast growth, high ornamental value, high stress resistance, high environmental protection performance and wide application prospects.

The invention discloses a method for increasing the slandscape liriodendron chinense transplantation survival rate. The method comprises the following steps of 1, pretreatment before transplantation;2, transporting pretreatment during transplantation; 3, tree transporting process; 4, transplanting site treatment; 5, post-maintenance of transplantation. According to the growth habit of slandscapeliriodendron chinense, from transplantation pretreatment to the transportation process to final aftertreatment of transplantation, it is guaranteed that a tree body is in the most comfortable state all the time, a method of hanging tree root subsoil and performing hanging bag infusion is adopted, it is guaranteed that the transpiration problem and the low resistance of the tree body in the long-term transportation process are solved, for a field planting pit of a transplantation site, the bottom layer belongs to an impervious layer, and water and fertilizer conservation is facilitated; a plant-derived additive is added on the surface layer, the tree body is protected against pests and diseases, and the problem that the survival rate in new areas of transplantation of shaped slandscape liriodendron chinense is low is solved.

The invention provides a grafting method of liriodendron chinense and relates to the technical field of planting grafting. The grafting method of the liriodendron chinense disclosed by the invention comprises the following steps: preparing scions; preparing rootstock; grafting; performing final-period management of grafted seedlings. According to the grafting method of the liriodendron chinense disclosed by the invention, 1-2 years of wild liriodendron chinense are used as the scions, the scions are semi-lignified, development of the scions is full, and more nutrient substances are stored, andtherefore, the grafting method is suitable for grafting big rootstock and has the advantages of quick sprouting, early formation of tree crowns, smooth grafting healing part and high grafting survival rate.

The invention discloses an aureobasidium aureum MR-57 and application thereof, and belongs to the field of functional microorganism screening and application, the strain MR-57 is preserved in Guangdong Microbial Culture Collection Center on November 26, 2021, and the preservation number is GDMCC No: 62084. The bacterial strain MR-57 has relatively strong inhibition and antagonism effects on seven pathogenic bacteria such as column spore destroying bacteria and the like, and has relatively strong antagonism capability on liriodendron alternaria alternata, column spore destroying bacteria and mechanical bacteria echinacea; the sterile fermentation liquor of the strain MR-57 has an obvious inhibition effect on the growth of fusarium equisetum hyphae and can obviously reduce the spore germination rate of fusarium equisetum; a soil mixing method is used for verifying that the strain MR-57 has good colonization capability in soil; a potted plant biocontrol test proves that the strain MR-57 has a certain prevention and growth promoting effect on the divaricate saposhnikovia root rot, and the disease index of the divaricate saposhnikovia root rot can be obviously reduced.

The invention discloses a culture medium using gamma-aminobutyric acid to promote hybrid liriodendron somatic embryogenesis and application thereof and belongs to the technical field of plant tissue culture. The culture medium is prepared by using 5mg / L of 3 / 4MC+VC and 30g / L sucrose as the basic culture medium and adding 0-10mg / L of gamma-aminobutyric acid, wherein the added gamma-aminobutyric acid can promote hybrid liriodendron callus somatic embryogenesis, accelerate somatic embryo development and increase somatic embryogenesis efficiency. When the culture medium is applied to hybrid liriodendron somatic embryogenesis and tissue culture, high somatic embryogenesis quantity and quality and fast somatic embryogenesis can be achieved, induced somatic embryo seedling number is extremely evidently higher than that of control, lateral root number and root length are evidently higher than those of the control, each callus can averagely form 20 regenerated plants, and the culture medium isquite high in practical application value.

The invention discloses a method for somatic embryogenesis of Liriodendron chinensis by using methyl jasmonate, including seed sterilization, induction of embryogenic callus, establishment of embryogenic suspension cell lines, and acquisition and transition of liquid single cells . The step of inducing the occurrence of somatic embryos of Liriodendron chinensis, characterized in that, when inducing the somatic embryos of Liriodendron chinensis, methyl jasmonate and ABA are added to the culture medium, or methyl jasmonate is added separately. The method for utilizing methyl jasmonate of the present invention to carry out hybrid Liriodendron body rapid propagation, in the hybrid Liriodendron somatic embryogenesis process, adding plant growth regulator MeJa effectively improves somatic embryo induction rate, reduces deformity rate, and further It is very practical to improve the somatic embryogenesis efficiency of Liriodendron hybridum, and it is beneficial to the establishment of rapid propagation system of Liriodendron hybridum.

The invention discloses a regeneration method adopting pure solid culture for a somatic embryo plant of liriodendron chinense. The method comprises steps as follows: 1) hybrid liriodendron chinense embryonic calli are induced; 2) 3 / 4 MS, VC with concentration of 5mg / L, saccharose with concentration of 30g / L and amino oligochitosan with concentration of 0.01-0.1mg / L are taken as basic media, and the hybrid liriodendron chinense embryonic calli are cultured in dark at 24 DEG C; 3) the material is transferred to a tissue-culture vessel in a light culture room and cultured continuously with the 3 / 4 MS basic medium until the regeneration plant is formed; 4) the regeneration plant is acclimated and transplanted. According to the regeneration method adopting pure solid culture for the somatic embryo plant of liriodendron chinense, the pure solid culture manner is adopted, the somatic embryo induction links including suspension culture and plate culture in the somatic embryo generation process are skipped, a hybrid liriodendron chinense somatic embryo generation system is optimized and enriched, and accordingly, the high-quality somatic embryo regeneration plant is obtained more efficiently and more conveniently; a stable and high-frequency hybrid liriodendron chinense somatic embryo generation system adopting pure solid media and having properties of being easy to operate and good in seedling resistance is established, and reference is provided for establishment of a more efficient genetic transformation receptor system of hybrid liriodendron chinense.

The invention relates to an asexual propagation method of michelia compressa. The asexual propagation method includes the steps that firstly, seedlings of the michelia compressa are manufactured; secondly, pretreatment of a scion of michelia compressa is performed; thirdly, a grafting method is adopted; fourthly, management after grafting is performed. Liriodendron chinense is used as a rootstock, and the cut-grafting method is used for grafting the michelia compressa. After cutting is sequentially soaked into a plant growth regulator and 1%DMSO, the rootstock is inserted, and after the cambium of the scion and the cambium of the rootstock are aligned, an anti-drop film is used for tightly winding and wrapping a port from bottom to top. According to the asexual propagation method of the michelia compressa, a great number of root-included seedlings of the michelia compressa can be obtained, the rate of reproduction is 81-86 times, and after the seedlings are obtained, the seedlings are grafted on the Liriodendron chinense, namely the rootstock, and the survival rate is 95%. After transplantation on a field is achieved for two years, the tree vigor is strong, a tree is resistant to diseases and capable of wintering safely, the problem of vacancy of the market resource of the michelia compressa is effectively solved, and the asexual propagation method of the michelia compressa is wide in application prospect.