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Liriodendron hybrids LhRGL1 gene and application thereof

A technology for hybridization of Liriodendron tulipifera and genes, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problems of slow progress, unknown structure and function, late research start, etc. The effect of increasing numbers

Active Publication Date: 2016-01-06
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the late start and slow progress of the research on woody plants, the number of genes encoding the DELLA protein in the hybrid tulip tree genome is still unclear, and its structure and function are unknown

Method used

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  • Liriodendron hybrids LhRGL1 gene and application thereof
  • Liriodendron hybrids LhRGL1 gene and application thereof
  • Liriodendron hybrids LhRGL1 gene and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The material used in this example is the hybrid Liriodendron tulipifera leaves on the campus of Nanjing Forestry University, which were quickly frozen in liquid nitrogen after harvest and stored in an ultra-low temperature refrigerator (-80°C).

[0025] 1) Extraction of total RNA from hybrid tulip tree leaves

[0026] According to the instructions of the NORGEN plant total RNA extraction kit, the specific operation is: add appropriate amount of materials and liquid nitrogen to the treated mortar, after fully grinding, add 600μL LysisSolution; transfer the lysed liquid in the mortar to a new In a centrifuge tube; after tightly closing the cap, invert and mix several times, let it stand at room temperature for 2 minutes to fully lyse, centrifuge at 12000rpm for 2 minutes, and transfer the supernatant to a new centrifuge tube; add an equal volume of 70% ethanol , vortex and mix well; transfer the mixture to the column provided in the kit (connected to a 2mL collection tube...

Embodiment 2

[0050] Research indicates LhRGL1 and wxya It plays an important role in various tissues and organs of Liriodendron hybrida. However, the expression levels of the two genes were the highest in the stem, indicating that the GA signaling pathway was active during the growth and development of the stem, and DELLA protein played an important regulatory role in the elongation and growth of the stem.

[0051] The plant material used in this embodiment is Arabidopsis ( Arabidopsisthaliana )Col (Columbia) wild-type seeds, donated by Professor Thomas Laux from Germany.

[0052] The Escherichia coli strain used in this embodiment is E. coli JM109; Agrobacterium strains GV3101 and EHA105 were used to transform Arabidopsis thaliana; the plant expression vector used in the experiment was pBI121. All strains were purchased from Related Biological Company.

[0053] 1) LhRGL1 Construction of gene pBI121 vector

[0054] With embodiment 1 obtaining LhRGL1 The full-length ORF sequen...

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Abstract

The invention discloses a liriodendron hybrids LhRGL1 gene and application thereof. The nucleotide sequence of the LhRGL1 gene is shown as SEQ ID NO.1, and the amino acid sequence of expression protein of the LhRGL1 gene is shown as SEQ ID NO.2. By cloning and authenticating the liriodendron hybrids LhRGL1 gene and analyzing the expression of the gene, functions of the gene are verified, it is found that an LhRGL1 transgenic plant is slow in growth and development, dwarfed and plenty in branch, and it can be seen that the gene has wide application in plant breeding.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to a hybrid Liriodendron LhRGL1 gene and an application thereof. Background technique [0002] Hybrid Liriodendron is a famous forest tree genetic breeder in my country, Professor Ye Peizhong, who uses the Chinese Liriodendron ( Liriodendron chinensis (Hemsl.) Sarg. ) and Liriodendron tulipifera ( LiriodendrontulipiferaLinn. ) is an interspecific hybrid obtained through artificial hybridization, which has fast growth, beautiful tree posture, peculiar leaf shape, strong resistance, and fine texture texture. It is an important tree species for landscaping and industrial timber. Although hybrid Liriodendron has many advantages, hybrid seed production is limited by season and efficiency, which affects the popularization and application of hybrid Liriodendron. Due to the large number of plant somatic embryos, once the somatic embryos are formed, they can q...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82C12N5/10A01H5/00
Inventor 陈金慧王鹏凯闫姗施季森董艺妮盛宇郑晨
Owner NANJING FORESTRY UNIV
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