13 results about "Arabidopsis" patented technology

The invention relates to the technical field of particle track microscopic analysis, in particular to a heavy ion track positioning method suitable for tiny seeds. The method mainly comprises the following steps of: scanning biological stacks by using a scanner, processing a scanning image by using drawing software to obtain an image of a single seed hole, acquiring position information of seeds in the seed holes, scanning a CR39 detection sheet by using a VHX microscope, acquiring passive detection information of each seed hole, and superposing the passive detection information with the image processed by the drawing software to obtain a final detection result. The radiation detection condition at the arabidopsis thaliana seeds is analyzed, and data conversion is further carried out to obtain various radiation information of individuals aiming at the arabidopsis thaliana seeds. The method is applicable to batch, rapid and high-precision processing of returned detection piece data. And in the aspect of the batch degree, the whole process is batch processing, and batch pictures and data can be automatically output by utilizing related software, so that the manual participation degree is reduced.

The invention belongs to the technical field of plant heredity and genetic engineering, and particularly relates to a cold-resistant and bolting-resistant related gene BraTZF3 of Chinese cabbage and application of the cold-resistant and bolting-resistant related gene BraTZF3. A stress-related transcription factor BraA01g008400.3 C is screened by combining a yeast stress library screening technology with transcriptome analysis, the stress-related transcription factor BraA01g008400.3 C belongs to a co-tandem C3H type zinc finger protein and comprises a C-x8-C-x5-C-x3-H zinc finger (ZNF) structural domain, and tests prove that the BraTZF3 is positively correlated to low-temperature stress response and cold resistance, and the BraA01g008400.3 C can be used for preparing the stress-related transcription factor BraA01g008400.3 C. According to the present invention, the BraTZF3 is subjected to heterologous overexpression to positively regulate the cold resistance and the bolting resistance of arabidopsis thaliana, such that the gene provides the positive regulation effect on the cold resistance and the bolting resistance of the plant, especially the Chinese cabbage, and the new tool and the new method are provided for improving the cold resistance and the bolting resistance of the crop by using the gene engineering means so as to provide the good practical application value;

The invention relates to a method for generating a radiation effect on plants by low-dose-rate gamma radiation, and belongs to the technical field of biological radiation. Comprising a plant screening mode plant, a food chain related plant and an ICRP recommended reference organism. And a gamma irradiation source selected for gamma irradiation is 60Co or 137Cs. And the gamma irradiation dose rate is less than 50mGy / h. The implementation effect of the method shows that the antioxidant enzyme system of arabidopsis thaliana can be activated by low-dose-rate irradiation. The method provides an important basis for evaluating the influence of low-dose radiation on the plant biological effect. In the future, low-dose-rate irradiation can be further expanded to be used for adjusting a complex relation between plant population ecological effects and mining adaptive application of plants under radiation stress, for example, a method for monitoring adverse effects of nuclear facility effluents on plant populations can be established through the method, and the method plays a positive role in ecological system stability. Meanwhile, the method disclosed by the invention can be used for plant stress tolerance breeding or seed selection.

The invention belongs to the technical field of plant biology, and particularly relates to a coding gene of a phoebe bournei PbWRKY55 transcription factor, a recombinant expression vector, a recombinant bacterium, application and a method for improving plant traits. The nucleotide sequence of the coding gene of the phoebe bournei PbWRKY55 transcription factor is as shown in SEQ ID NO. 1. By increasing the expression quantity of the coding gene of the phoebe bournei PbWRKY55 transcription factor, the phosphorus absorption of transgenic arabidopsis thaliana can be effectively improved, the symptoms caused by low-phosphorus stress of plants are relieved, and the tolerance of the phoebe bournei PbWRKY55 transcription factor to a low-phosphorus environment is improved. And a new strategy is provided for improving the low-phosphorus-resistant property of the plant and increasing the phosphorus absorption and utilization efficiency of the plant by utilizing a genetic engineering means in the future.

1.The name of the design product: flower device (mengqu ling cat). 2.The use of the design product: decorative ornaments. 3.The design points of the design product: the combination of shape, pattern and color. 4.The picture or photo that best shows the design points: perspective view. 5.The design claimed contains color.

The invention discloses an oilseed rape BnaC07. WRKY7 gene and application of the oilseed rape BnaC07. WRKY7 gene in regulation and control of the content of phytosterol, and belongs to the technical field of plant genetic engineering, and the nucleotide sequence and the amino acid sequence of the oilseed rape BnaC07. WRKY7 gene are shown as SEQ ID NO: 1 and SEQ ID NO: 2. The invention reports the function and application of the BnaC07. WRKY7 gene in the synthesis of phytosterol for the first time. Through construction of a gene overexpression vector, the gene overexpression vector is transiently expressed in tobacco leaves, so that the total sterol content is remarkably increased; in an arabidopsis thaliana T-DNA mutant, the total sterol content of the seeds is obviously reduced; overexpression is carried out in oilseed rape, and the total sterol content in rapeseeds and leaves is remarkably increased. The gene is proved to have the functions of positively regulating and controlling sterol synthesis and further improving the content of phytosterol in rapeseeds and leaves, which is of great significance in analyzing a genetic regulation and control mechanism of the content of sterol in the rape, cultivating a new variety of rape with high phytosterol and improving the nutritional quality of the rape.

The invention provides a culture method of symbiotic bacteria for promoting growth of Arabidopsis arundinacea and application of the symbiotic bacteria. The culture method comprises the following steps: (1) sterilizing a symbiotic bacteria culture medium at 120-125 DEG C for 10-20 minutes; (2) respectively adding the alternaria alternata BB-1, the alternaria brassicae BB-6 and the alternaria hexaphoria BB-8 into the symbiotic bacterium culture medium; and keeping the temperature of the symbiotic bacterium culture medium at 29-30 DEG C, firstly carrying out aerobic fermentation culture for 6-7 hours, then carrying out anaerobic fermentation culture for 3-4 hours, and repeating the steps for 50-80 hours to obtain a symbiotic culture product. According to the invention, DSE of alternaria alternata BB-1, DSE of alternaria brassicae BB-6 and DSE of alternaria hexangularis BB-8 are used to establish a reciprocal symbiotic relationship with host plant Arabidopsis arundinacea in a long-term ecological adaptation process.

The invention belongs to the technical field of plant heredity and genetic engineering, and particularly relates to a Chinese cabbage bolting-resistant gene BraDREB1D and application thereof. Specifically, a low-temperature-resistant key transcription factor BraDREB1D (BraA10g010540.3 C) induced by low temperature is found through combination of a yeast stress library screening technology and transcriptome analysis, belongs to an AP2 type transcription factor, and has a relatively close genetic relationship with arabidopsis thaliana CBF4 / DREB1D in evolution, so that the key transcription factor is named as BraDREB1D. Experiments prove that the key transcription factor can effectively improve the cold resistance of plants, and the application of the key transcription factor BraDREB1D in the field of plant growth. Therefore, the method can be used for improving the quality and yield of plants (such as Chinese cabbages). Meanwhile, the BraDREB1D is used as the marker, so that the performance of plant germplasm, especially the germplasm performance of Chinese cabbage, can be identified, and therefore, the BraDREB1D has a good practical application value.

The utility model discloses a paddy rice seedling stage adversity hydroponic device relates to paddy rice hydroponic field, including front plate, shading flashboard hand-held position and mobile seed planting groove hand-held position, one side of the front plate is fixedly connected with right side plate, the other side of the front plate is fixedly connected with left side plate, one side of the front plate is fixedly connected with bottom plate, and the other side of the front plate is fixedly connected with the mobile seed planting groove hand-held position. The side, away from the front plate, of the bottom plate is fixedly connected with a back plate, one side of the right side plate is connected with a shading insertion plate in an inserted mode, the front side face of the bottom plate is provided with scale marks, and the top face of the back plate is provided with scale marks. Under the condition that root systems are not damaged, arabidopsis seeds can be prevented from falling off, and the germination rate is increased.

This invention discloses the application of the *Rhizopus dentata* ScPRT1 gene in regulating plant responses to abscisic acid (ABA), the nucleotide sequence of which is shown in SEQ ID NO:1. This invention found that *Arabidopsis thaliana* lines overexpressing the *Rhizopus dentata* ScPRT1 gene, or *Rhizopus dentata* lines transiently expressing the *Rhizopus dentata* ScPRT1 gene, exhibited significantly reduced ABA sensitivity and significantly enhanced ABA response. Therefore, the *Rhizopus dentata* ScPRT1 gene negatively regulates plant responses to ABA signals. This invention provides resources for the discovery of stress-resistance genes, improves the regulatory network of *Rhizopus dentata* in response to ABA signals, and offers a new perspective for understanding the molecular mechanisms of stress adaptation in desert bryophytes.

The invention provides a cruciferous plant cell membrane vesicle. The cruciferous plant cell membrane vesicle is a nano vesicle which is prepared from a cell membrane extracted from a cruciferous plant through a membrane extrusion technology and has the diameter of 50-100nm; the cruciferous plant is any one of arabidopsis thaliana, white radish, broccoli, cabbage or isatis tinctoria. The invention also provides a method for preparing the nano-vesicles, which comprises the following steps: degrading cell walls of cruciferous plants by using cellulase and macerozyme, swelling and breaking plant cells by using a hypotonic solution, carrying out differential centrifugal separation on plant cell membranes, and preparing the vesicles with the diameter of 50-100nm by using a liposome extruder. The invention also provides an application of the nano vesicle in treatment of inflammatory bowel diseases. The cruciferous plant cell membrane vesicle retains LORE protein, can specifically adsorb endotoxin, can prevent or save lipopolysaccharide induced cell inflammation, and can also effectively relieve mouse inflammatory bowel diseases.

The invention discloses actinidia eriantha endophytic fungi and application thereof, and belongs to the technical field of plant growth promotion. The endophytic fungi are separated from living actinidia eriantha of actinidae plants by adopting a traditional endophytic fungi separation and purification technology, belong to ascomycophyta, hypocrea, erythrinae and spiropolyspora fungi, and are preserved in the China General Microbiological Culture Collection Center on March 8, 2024, the strain is classified and named as the powdery spiropolyspora farinosa AER17, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.41113. The invention further discloses a preparation method of the powdery spiropolyspora farinosa AER17. The invention also discloses an application of the endophytic fungi: the expression of Arabidopsis thaliana growth related genes DPE2, PHS1, G6PD3 and G6PD5 is improved, the growth of a model plant Arabidopsis thaliana is promoted, and the fresh weight is improved by 149.20% (Plt; 0.01), the root length is increased by 47.39% (Plt; 0.01) of the substrate.

The invention discloses a phoebe bournei MADS33 gene, and the phoebe bournei MADS33 gene has a nucleotide sequence as shown in SEQ ID No: 1, or a sequence which is obtained by deleting, adding and / or substituting one or more nucleotides of the sequence and has an unchanged function. The invention also discloses a protein coded by the phoebe bournei MADS33 gene, and the protein is composed of amino acids as shown in SEQ ID No.2. The invention further discloses a method for advancing plant flowering. The invention also discloses an application of the phoebe bournei MADS33 gene in advancing plant flowering. The phoebe bournei SOC1 gene member MADS33 is obtained by screening based on transcriptome data, the biological function of the phoebe bournei SOC1 gene member MADS33 is verified through heterologous transgenic arabidopsis thaliana, the fact that the phoebe bournei MADS33 gene can advance plant flowering is revealed, and the phoebe bournei MADS33 gene is expected to artificially advance plant flowering.