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Application of Arabidopsis glycosyltransferase gene UGT73C7 in improving plant disease resistance

A technology of glycosyltransferase and Arabidopsis sugar, applied to the application of glycosyltransferase gene, the application field of Arabidopsis glycosyltransferase gene UGT73C7 in improving plant disease resistance, can solve the problem of glycosyltransferase The special function of the gene has not been revealed, etc., to achieve the effect of improving disease resistance

Inactive Publication Date: 2015-08-19
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the sequences of these glycosyltransferase genes have been published in the nucleic acid sequence database, the special functions of these glycosyltransferase genes have not been revealed. For example, the UGT73C7 gene in Arabidopsis glycosyltransferase family 1 can enhance The function and application of transgenic plant disease resistance have not been reported so far

Method used

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  • Application of Arabidopsis glycosyltransferase gene UGT73C7 in improving plant disease resistance
  • Application of Arabidopsis glycosyltransferase gene UGT73C7 in improving plant disease resistance
  • Application of Arabidopsis glycosyltransferase gene UGT73C7 in improving plant disease resistance

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Embodiment 1

[0021] Example 1 Cloning of Arabidopsis Glycosyltransferase Gene UGT73C7

[0022] 1. Cloning of Arabidopsis glycosyltransferase gene UGT73C7

[0023] The cDNA sequence of the UGT73C7 gene was obtained through the public website http: / / www.cazy.org. Primers were designed according to the cDNA sequence, the forward primer was 73C7-F:5'-GGATCCCTTTCTACAATGTGTTCTCATGATCC-3', and the reverse primer was 73C7-R:5'-GAGCTCGTTTTTCTCTGGTGCCAAATACC-3'. Arabidopsis RNA was extracted using TRIzol kit, and the full-length cDNA sequence of UGT73C7 gene was amplified by RT-PCR. The process of cloning the cDNA was first digested with BamHI and Sac I, and then connected into the pBluescript II SK(+) vector digested with the corresponding enzymes to construct a sequencing intermediate vector called pK73C7. Then PCR amplification of the target gene and verification of BamHI and Sac Ⅰ enzyme digestion were carried out on the vector, and finally the sequence was determined to verify the correctness...

Embodiment 2

[0026] Example 2 Transgenic application of Arabidopsis thaliana glycosyltransferase gene UGT73C7

[0027] 1. Construction of cDNA expression vector containing UGT73C7 coding region

[0028] After the pK73C7 intermediate sequencing vector was digested with BamHI and SacⅠ, the full-length cDNA sequence with the cohesive ends was obtained. The gene fragment was connected with the pBI121 carrier part digested with corresponding enzymes to obtain a plant expression vector driven by the CaMV 35S promoter to overexpress the glycosyltransferase gene, which was called pBI73C7.

[0029] 2. Agrobacterium-mediated plant transformation

[0030] Agrobacterium GV3101 has the ability to infect plants and transfer genes, so the constructed UGT73C7 plant expression vector (pBI73C7) was transformed into Agrobacterium, and then verified by PCR and enzyme digestion. Arabidopsis flower buds were infiltrated with Agrobacterium GV3101 containing a plant expression vector using the flower dip method...

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Abstract

The invention discloses an application of Arabidopsis glycosyltransferase gene UGT73C7 in improving plant disease resistance, wherein the nucleotide sequence of the glycosyltransferase gene UGT73C7 is shown in SEQ ID No. 1 and is cloned by RT-PCR from Arabidopsis thaliana. The ene UGT73C7 is used to construct a plant overexpressing vector for plant transgene operation, so as to obtain the transgenic plants. The detection results show that the disease resistance of the transgenic plants is significantly improved, suggesting that new disease-resistant plants can be created after the implementation of the application disclosed by the invention, and the application can be used for the subsequent crop variety improvement and has great significance for China's agricultural production.

Description

technical field [0001] The invention relates to the application of a glycosyltransferase gene, in particular to the application of an Arabidopsis thaliana glycosyltransferase gene UGT73C7 in improving plant disease resistance, and belongs to the field of genetic engineering. Background technique [0002] Pathogenic bacteria (living bacteria, semi-living bacteria, dead bacteria) are ubiquitous in nature, and they enter plants through their own different ways (such as through stomata, water pores or injured parts of epidermal cells) and lodge in plants. In different parts of the plant (intercellular or across the plasma membrane into the cell) (Jones at el., 2006). Phytopathogenic bacteria residing in plants proliferate in large numbers and cause serious plant diseases. [0003] In agriculture, plant diseases have always been difficult to prevent and control in production. According to the estimates of the Food and Agriculture Organization of the United Nations (FAO), the ann...

Claims

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Application Information

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IPC IPC(8): C12N15/54C12N15/84A01H5/00
Inventor 侯丙凯王勇李燕洁
Owner SHANDONG UNIV
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