The invention discloses a custom customization method of an anti-aging skincare product. The custom customization method comprises the following steps: step 1, collecting user cell samples; step 2, extracting mRNA in the cell samples; step 3, performing reverse transcription for the mRNA to synthesize cDNA; step 4, detecting polymorphism of aging-related genes in cDNA and expression of corresponding cDNA, wherein the aging-related gene contains at least one of MMP9, NQ01, SOD1 and FOXO4; and step 5, judging the anti-aging capacity according to the gene polymorphism and the corresponding cDNA expression, selecting anti-aging skincare product components and use amount according to the anti-aging capacity, and preparing the appropriate anti-aging skincare product. The anti-aging capacity of different users is acquired by measuring the expression of the aging-related gene, and the appropriate anti-aging skincare product is prepared according to the specific anti-aging capacity of the user.