Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Hepatitis B virus vaccine synergistic protein and its gene

A technology of hepatitis B virus and synergistic protein, applied in the direction of virus, antiviral agent, genetic engineering, etc., can solve the problems of big difference, inability to produce enough hepatitis B virus, and reduce the injection dose and frequency, enhance the Immunity effect, effect of increasing titer

Inactive Publication Date: 2007-04-18
FUDAN UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the immune effect of hepatitis B vaccine varies greatly among individuals in the population. After three routine injections, many people cannot produce enough antibodies to resist hepatitis B virus infection and hepatitis B disease.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hepatitis B virus vaccine synergistic protein and its gene
  • Hepatitis B virus vaccine synergistic protein and its gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] The acquisition of the protein gene: using the complete HBsAg as the screening molecule, the human liver cDNA expression library was screened by the immunoblotting method, and after a certain number of clones were screened, a positive cDNA clone was finally obtained. The cDNA was sequenced and analyzed, and it was found that the cDNA had an independent open reading frame (ORF), the size of the entire ORF was 1035bp (SEQ.ID.NO.1), and the amino acid sequence of the encoded protein was SEQ.ID.NO.2.

[0015] Genetic engineering expression and purification of the protein: the gene is cloned into a eukaryotic or prokaryotic expression vector system for expression and purification. For example, 6×his purification tag and enterokinase cleavage site DDDDK are introduced at the N-terminus of the cDNA. The 6×his purification tag is conducive to the affinity purification of the fusion protein of interest, and the enterokinase cleavage site can make the purified fusion protein The ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

This invention relates to a hepatitis B virus vaccine synergic protein and its genes, and utilizes gene engineering principle to express the method and application of the protein. CDNA of the protein is gained by screening from human liver CDNA expression bank, after sequence analysis ,using the way of gene engineering to clone to prokaryotic cell, eukaryotic cell(animals, plants) to express protein by coding of the CDNA ,for instance ,cloning CDNA to prokaryotic expression vector and gaining recombination protein by expression depuration in Bacterium coli. Combination of the protein and hepatitis B virus vaccine can improve vaccine immune effectiveness, reinforcing the immunity of hepatitis B virus infectors, increasing antibody titer. Protein of this invention can be used as adjuvant to hepatitis B vaccine.

Description

technical field [0001] The invention relates to a hepatitis B virus vaccine synergistic protein and its gene, as well as a method and application for expressing the protein by using the principle of genetic engineering. Background technique [0002] Hepatitis B is a relatively serious infectious disease that threatens human health at present. It has a high incidence and great harm in my country. Inoculation of hepatitis B vaccine is the most effective measure to prevent hepatitis B infection and reduce the carrier rate of hepatitis B virus. However, the immune effect of hepatitis B vaccine varies greatly among individuals in the population. After three routine injections, many people cannot produce enough antibodies to resist the infection of hepatitis B virus and the onset of hepatitis B. Therefore, finding molecules that can effectively enhance the immunogenicity of hepatitis B vaccine is an important way to solve this problem. [0003] Using the principles of genetic en...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07K14/435C12N1/21A61K38/17
CPCA61K38/00A61K39/292A61K2039/523C07K14/4705A61K2039/55516C12N2730/10134A61K39/12A61P31/20A61P37/04
Inventor 朱乃硕李华青杨敏
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com