39 results about "In vitro stimulation" patented technology

A method including in vitro stimulating a core cell population (CCP) of at least 5 million cells that have a density of less than 1.072 g/ml, and at least 1.5% of which are CD34+CD45−/dim, to differentiate into a neural progenitor/precursor cell population (NPCP). Other embodiments are also described.

The invention discloses a preparation method and application of a magnetic nano microcarrier for wrapping a tumor cell membrane. The method is based on the tumor cell membrane, and the surface of a ferroferric oxide nano particle modified by silicon dioxide is wrapped with the cell membrane derived from a tumor cell through a membrane-nano coextrusion and centrifugation method, so that the Fe3O4@SiO2 magnetic nano microcarrier (CMNPs) wrapped with the tumor cell membrane is formed. The magnetic nano microcarrier contains a tumor cell membrane surface antigen, has superparamagnetism, and can beenriched and purified by a magnetic field, so that the magnetic nano microcarrier can stimulate activation of immune cells in vitro or is injected into a body to be captured by macrophages to improvea tumor specific antigen, and the anti-tumor effect is enhanced. The preparation method of the microcarrier based on the cell membrane has the advantages of simplicity and easiness in operation, lowcost, wide application and the like.

The invention discloses an immune adjuvant of a foot-and-mouth disease vaccine and application of the immune adjuvant, and belongs to the field of biological vaccines. The immune adjuvant is a 3D protein fragment of foot-and-mouth disease viruses. Experiments prove that after the expressed 3D protein fragment is compatible with antigens, animals are immunized, the immunogenicity of multi-epitope antigens can be enhanced, and high-level protective neutralizing antibodies are produced. In vitro stimulation shows that after the 3D protein fragment of the foot-and-mouth disease viruses is separately adopted or compatible with the multi-epitope antigens, lymphocyte proliferation response can be produced, namely the immune adjuvant can be used for enhancing immune response, distinguishing infection and immunizing the animals and is a good immune stimulant and a vaccine molecular marker.

The invention relates to a medical suture friction testing apparatus. The apparatus comprises an assembly for testing the friction performance of a medical suture when the medical suture penetrates tissues, and an assembly for testing the friction performance between medical sutures. The assembly for testing the friction performance of a medical suture penetrating tissues stimulates the friction performance between the medical suture and all tissues when the medical suture penetrates human tissues, and the assembly for testing the friction performance between medical sutures stimulates the friction assembly between the medical sutures in the knotting process of the medical suture. The two assemblies can be arranged in a multifunctional fiber strength and elongation tester to replace a lower clamping head of the tester in order to carry out relevant friction performance test. The invention also discloses a corresponding medical suture friction testing method. The friction testing apparatus has the advantages of simple structure, convenient operation, and objective and real result. The apparatus and the method fill up a blank in the quantitative characterization of present in-vitro stimulation of the friction performance of the medical suture, and provide scientific bases for the improvement of the structure of the medical suture.

The invention discloses a wireless program-controlled illumination system used for cell high-throughput research of optogenetics. The system comprises an upper computer and control software, a wireless communication module, a lower computer central processor, a lower computer register chip set, a lower computer light emitting diode group and a constant-voltage direct-current power supply. The device provided by the invention is used for the research for the optogenetics signal channel of the in-vitro large-scale culture of cells, especially the research for drug molecule screening relevant to the signal channel, the problem that in the current relevant researches, the devices for realizing high-throughput, multi-intensity, long-time and multi-factor illumination in-vitro stimulation are not available is solved, and the accuracy and experiment efficiency of the light stimulation system in the optogenetics research are effectively improved. In addition, the cell culture light activating conditions acquired based on the system provided by the invention, such as the illumination intensity and the light activating time, have wide application prospects for the optogenetics research of the cells in the future.

This invention provides cells and methods for stimulating proliferation and migration of endogenous and exogenous mammalian stem cells in vivo and in vitro. The invention provides reagents and methods for efficiently proliferating mammalian stem cells in an animal in need thereof and producing stem cells that can be re-introduced into an animal in need thereof to alleviate neurological and corporal disorders.

The invention provides a method for regulating the polarization state of macrophages. The method comprises the step of activating cholinergic anti-inflammatory pathways of the macrophages by adoptingcholinergic anti-inflammatory pathway agonists and/or vagus nerve electrical stimulation signals. According to the method in the invention, the cholinergic anti-inflammatory pathway agonists are adopted to stimulate the macrophages in vitro; or the electrical stimulation signals are adopted to stimulate vagus nerves in vivo; therefore, the cholinergic anti-inflammatory pathways are activated; polarization of M1 type macrophages is inhibited; polarization of M2 type macrophages is promoted; a regulating effect on macrophage polarization types is achieved; and the method has the important significance in the aspects of specifically regulating the functions of the polarized macrophages and diseases caused by the polarized macrophages.

Disclosed are methods of obtaining a cell population enriched for T cells having antigenic specificity for a cancer-specific mutation using in vitro stimulation of memory T cells. Also disclosed are related methods of isolating a T cell receptor (TCR), populations of cells, TCRs or antigen-binding portions thereof, pharmaceutical compositions, and methods of treating or preventing cancer.

The invention belongs to the technical field of inhibition of hypoxia spermatogenesis reduction, and discloses a detection method for inhibition of hypoxia spermatogenesis reduction of DR5 by VHA. VHAgene knockout mice and C57BL/6 mice are used as research objects, and a hypoxia spermatogenesis reduction model is constructed through hypoxia induction. A seminiferous tubule seminiferous cell of anexperimental mouse is separated to serve as a research object. Hypoxic in-vitro stimulation is conducted, and the regulating effect and the molecular mechanism of VHA for inhibiting seminiferous cellexpression DR5 are verified through western blot, immunocytochemistry and qPCR technologies. According to the detection method for inhibiting DR5 spermatogenesis reduction under oxygen deficit by VHA, which is provided by the invention, the effect of hypoxic inhibition of VHA in hypoxic spermatogenesis reduction and the molecular mechanism of hypoxic inhibition of VHA in inhibition of DR5 expression are clarified, and a necessary theoretical basis and a new treatment strategy are provided for treatment of hypoxic spermatogenesis reduction.

The invention discloses a hepatoma AFP (alpha-fetoprotein) specific artificial antigen-presenting cell inducing reagent kit, belongs to the technical field of cytobiology and solves the problems of the prior art that in-vitro amplification of DC cells is difficult and acquisition of specific CTLs (cytotoxic T Lymphocytes) by in-vitro stimulation of CD8+T cells is low in efficiency. The kit hereincomprises an HLA-A2-Ig dimer, an anti-CD28mAb antibody, magnetic beads, and AFP polypeptides. The kit herein is effective in amplifying antigen-specific CTLs in vitro, with specificity maintained formonths; CD*+T cells are stimulated for hepatoma-specific aAPC, the CTLs account for 95% and above the total cell quantity after one week of cultivation, and the amplification times is 1000 and above.The cell quantity can be further increased by repeatedly stimulating the AFP specific CTLs via aAPC. The attained CTLs present effect memory cell features in appearance, and can secrete TNF alpha andIFN gamma.

The invention relates to the field of biological medicine, and in particular relates to application of pulsatilla saponin B4 to preparation of medicine for preventing and/or treating tumor. Results ofhemolysis tests show that the pulsatilla saponin B4 does not have the obvious hemolysis phenomenon during incitation by in vitro addition in rabbit 2-percent erythrocyte suspension for stimulation. According to the evaluation on toxicity of the pulsatilla saponin B4 to the in vitro cell, the condition that the pulsatilla saponin B4 does not have the obvious cell toxicity to the in vitro culturedRD cells is discovered through study. The acute toxicity tests are used for evaluating the safety of the pulsatilla saponin B4; test results show that the pulsatilla saponin B4 is safe and non-toxic.Through MTT experiments, the condition that the pulsatilla saponin B4 does not have the cell toxic effects on S180 and Lewis cancer cells, and cannot be used for inhibiting the tumor cell multiplication is discovered; but the results of mice in vivo transplantation tumor tests show that the pulsatilla saponin B4 obviously inhibits the growth of the S180 sarcoma growth in the bodies of the mice, and the anti-tumor effect is achieved in the bodies.

The present disclosure provides compositions and methods for promoting stem cell and/or progenitor cell proliferation and/or differentiation. The provided compositions may be useful in treating a disease or condition that is related to mucosal barrier function, e.g., wound healing, treating skin conditions (e.g., atopic dermatitis, psoriasis, bed sores, or condition related to the aging of skin), treating a lung disorders (e.g., asthma), a condition related to improving mucosal barrier function, and/or treating injury to GI mucosa in a subject in need thereof. The present disclosure also provides methods for promoting the proliferation and/or differentiation of stem cells and/or the progenitor cells in a subject in need of such treatment by administering a composition. The ability to stimulate the proliferation and/or differentiation of stem cells and/or the progenitor cells in vivo, ex vivo and/or in vitro provides tremendous benefit. The present disclosure can be used to increase stem cell populations in in vivo, ex vivo and/or in vitro. Stem cell transplantation provides treatments and/or cures of many disease states, degeneration and/or injury.