90 results about "Pig reproduction" patented technology

The invention belongs to the technical field of pig molecular marker-assisted selection, and particularly relates to application of an SNP molecular marker related to the character of the number of live piglets born by pigs. The SNP molecular marker is a basic group of a 32663785th site of a ninth chromosome in a pig genome Sscrofa11.1. 109, the SNP site of the molecular marker has C / T polymorphism, and T is a favorable allelic variation of the character of the number of live piglets born by a pig. The dominant alleles of the SNP are preferably selected, the frequency of the dominant alleles can be increased generation by generation, the number of live piglets of pigs is increased, and the genetic improvement progress of the pigs is accelerated, so that the breeding and production benefits of the pigs are effectively improved.

The invention discloses an automatic grading and grouping system and method for boars, and relates to the technical field of boar breeding, and the system constructs a multi-modal biological characteristic data set by collecting gene, semen quality, health examination and growth performance data. Secondly, extracting gene features by using a convolutional neural network, calculating a gene screening coefficient JYX, and classifying the gene screening coefficient JYX into an A class level and a B class level through a first threshold Q1; then, semen quality analysis is conducted on the A-class boars, a semen quality screening coefficient ZLX is calculated, and the A-class boars are divided into the A1-class boars and the A2-class boars through a second threshold Q2; and when the A2-class boar triggers health early warning, further evaluating a health index JCZ, and subdividing the health index JCZ into A21, A22 and A23 classes according to a third threshold Q3. And finally, grouping the boars according to the class levels of the boars, and formulating an accurate feeding strategy to optimize the reproductive performance and improve the breeding benefits.

The invention provides a triple inactivated vaccine for porcine circovirus type 2, porcine mycoplasmal pneumonia and porcine reproductive and respiratory syndrome as well as a preparation method and application of the triple inactivated vaccine, and belongs to the technical field of biology. The triple inactivated vaccine is prepared on the basis of a porcine circovirus type 2 protective antigen Cap protein, a mycoplasma hyopneumoniae protective antigen recombinant fusion protein F-P46-P65 and a porcine reproductive and respiratory syndrome virus PRRSV-NADC30-like LF strain. According to the triple inactivated vaccine, three major diseases caused by the porcine circovirus type 2, the mycoplasma hyopneumoniae and the porcine reproductive and respiratory syndrome virus can be prevented at the same time through one-time inoculation, the immunization procedure is greatly simplified, the stress reaction caused by multiple immunization to pigs is effectively reduced, and the healthy growth of the pigs is guaranteed. The immune cost of a farm is remarkably reduced, the effect of'immune burden reduction 'is really achieved, and the efficiency and economic benefits of breeding management are improved.

The invention discloses a complete feed suitable for sows in a perinatal period and a preparation method of the complete feed in the technical field of animal nutrition and feed science, and relates to a beta-glucan sulfated-chitosan conjugate and a nano-embedded quercetin-medium-chain fatty glyceride compound, and application of the beta-glucan sulfated-chitosan conjugate and the nano-embedded quercetin-medium-chain fatty glyceride compound in the complete feed. Wherein the beta-glucan sulfated-chitosan conjugate is formed by carrying out sulfated modification on beta-glucan through chlorosulfonic acid and then carrying out covalent coupling on the modified beta-glucan and chitosan under the conditions of a specific condensing agent and pH (Potential of Hydrogen). The complete feed contains the energy raw material, the protein raw material, the functional additive and the two modified compounds, and is prepared through the processes of step-by-step crushing, mixing and granulation, the complete cycle regulation of relieving constipation, promoting ingestion, reducing oxidative damage and improving lactation performance is finally achieved, and the breeding efficiency of sows and the health level of piglets are remarkably improved.

The invention belongs to the field of veterinary biological products, and relates to a porcine reproductive and respiratory syndrome virus strain and application thereof. The invention particularly discloses a porcine reproductive and respiratory syndrome virus strain with a microbial preservation number of CGMCC (China General Microbiological Culture Collection Center) No.47095. The invention also discloses a vaccine composition prepared from the strain. After pigs are inoculated with the vaccine composition, the vaccine composition can resist challenge of a porcine reproductive and respiratory integrated virus SD strain and a porcine reproductive and respiratory integrated virus HuN4 strain, and has application prospects.

The application belongs to the field of biological medicine, and particularly relates to a medicine for blue ear virus. The application mainly provides a medicine combination based on polyether long-chain antibiotics, which is used for treating and / or preventing a disease caused by a porcine reproductive and respiratory syndrome virus (PRRSV). The medicine of the application can efficiently inhibit the replication of the PRRSV, helps to maintain the health state and production performance of pigs, has a simple preparation method, is suitable for large-scale production, and provides a new and effective solution for the animal husbandry.

The invention provides an SNP molecular marker related to porcine reproductive traits in a porcine DDR2 gene, and belongs to the technical field of porcine molecular marker screening, and the reproductive traits are total litter size and live litter size. The SNP molecular marker is located in a pig DDR2 gene sequence, the nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO.1 in a sequence table, and a Ggt exists at the 265bp position of the sequence; a basic group mutation; the invention also provides a primer pair for amplifying the molecular marker and a method for detecting and judging porcine reproductive traits. The invention provides a new marker for molecular marker-assisted breeding of the porcine reproductive traits, and the marker can be utilized to realize rapid identification of the porcine reproductive traits so as to improve the efficiency of porcine breeding.

The invention discloses a clostridium butyricum preparation for preventing and treating porcine reproductive and respiratory syndrome and application of the clostridium butyricum preparation, and belongs to the technical field of microbial fermentation feed. According to the invention, the clostridium butyricum with the preservation number of CCTCC NO: M 20231636 is subjected to activation culture, seed culture and high-density fermentation culture to prepare the clostridium butyricum preparation, and the effect of the clostridium butyricum preparation in prevention of PRRSV infection is developed. Experimental results show that when the clostridium butyricum preparation is added into a pig complete feed, the positive infection rate of the porcine reproductive and respiratory syndrome of a pig herd can be effectively reduced, the death number of the pig herd is remarkably reduced, and the clostridium butyricum preparation has outstanding prevention and treatment effects. The clostridium butyricum preparation provided by the invention is free from drug resistance and toxic and side effect risks, has the advantages of low cost, high safety, environment friendliness and the like, and has great significance in benefit improvement of the live pig breeding industry.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker of an eleventh intron of a pig ARRB1 gene, a primer pair and application of the SNP molecular marker and the primer pair in pork quality character breeding, and relates to the technical field of pig molecular markers. Wherein the pork quality character is intramuscular fat character; the SNP molecular marker is located in an eleventh intron of an ARRB1 gene of a ninth chromosome of a pig, the nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO.1 in a sequence table, and g.130Cgt exists at the 130bp position of the sequence; the T basic group is mutated. The invention also provides a primer pair for amplifying the molecular marker and a method for breeding pigs by using the molecular marker. According to the invention, the correlation between one SNP molecular marker in the pig ARRB1 gene and the intramuscular fat character of the pig is explored for the first time, so that the SNP molecular marker can be used for marker-assisted selective breeding of the pig, that is, a new application of the SNP molecular marker in the pig ARRB1 gene in marker-assisted breeding of the pork quality character is provided, early screening of the breeding character of the pig is realized, and the breeding efficiency of the pig is improved. The screening method is simple and rapid.

This application discloses a circular RNA vaccine against porcine reproductive and respiratory syndrome (PRRS) virus, its construction method, and its application. The circular RNA vaccine comprises circular RNA and a pharmaceutically acceptable carrier. The coding elements in the circular RNA encode the GP4, GP2, GP5, and M proteins of PRRS virus. The circular RNA vaccine exhibits high stability and good safety, producing no adverse reactions after immunization of pigs. It induces high levels of neutralizing antibodies in pigs after immunization and activates a high level of cellular immune response, providing up to 100% immunoprotection. Furthermore, its preparation process eliminates the need for virus isolation, significantly shortening the research and development cycle. The production process is not constrained by biological factors such as virus titer, facilitating standardization and large-scale production, and effectively reducing production costs.

The invention discloses an application of gallnut tannic acid as an additive in boar production. The application of the gallnut tannic acid as the additive comprises the application of the gallnut tannic acid as a feed additive in improving the reproductive performance of adult breeding boars and the application of the gallnut tannic acid as a semen diluent additive in improving the normal-temperature preservation quality of boar semen. The feed additive can improve the reproductive performance of adult breeding boars, can significantly improve the sperm density, the total sperm number and the effective sperm number of the boars after being fed for 12 weeks, and improves the oxidation resistance of sperms, sperms and serum; the semen diluent additive can improve the normal-temperature preservation quality of the boar semen, and the sperm motility can still reach 61.20% after the boar semen is preserved for 7 days.

The application discloses a kind of sow reproductive behavior intelligent monitoring methods based on depth estimation, belong to wisdom breeding and animal husbandry science field, which includes the following steps: based on the original sow monitoring video is preprocessed to obtain several short video clips according to frame extraction rule;Several short video clips are extracted to obtain short video representative frame, and short clip attention mask chart is obtained by processing short video representative frame based on depth estimation algorithm;Several short video clips are reorganized to obtain long monitoring video, and long monitoring time clustering table is obtained by extracting features and clustering long monitoring video;Long monitoring video summary and analysis chart are formed based on short clip attention mask chart and long monitoring time clustering table.This application can efficiently process massive monitoring video data, accurately capture the key information of sow reproductive behavior, reduce the cost of artificial monitoring, improve monitoring efficiency and accuracy.

The present invention belongs to the field of biomedicine, and specifically relates to a novel drug against the porcine reproductive and respiratory syndrome virus. The present invention mainly provides a polyether long-chain antibiotic-based pharmaceutical combination, which is used for treating and / or preventing diseases caused by the porcine reproductive and respiratory syndrome virus (PRRSV). The drug of the present invention can efficiently inhibit the replication of PRRSV, and helps maintain the health state and reproduction performance of pigs. The preparation method is simple and convenient, is suitable for large-scale production, and provides a new and effective solution for the livestock industry.

The present application relates to the field of traditional Chinese medicinal materials, in particular to a composition for resisting PRRSv and improving the reproductive performance of sows and application thereof. The present application formulates a composition composed of 10 plant extracts (Angelica sinensis extract, Atractylodes macrocephala Koidz extract, Glycyrrhiza uralensis Fisch extract, Eucommia ulmoides Oliver extract, Eucommia ulmoides leaf extract, Populus davidiana Dode extract, Perilla frutescens leaf extract, Gynostemma pentaphyllum Makino extract, Leonurus japonicus extract and Astragalus membranaceus Bge extract) for sows in late pregnancy, and collects sow blood and placenta samples at the time of delivery, studies the influence of the composition on sow reproduction, and explores the mechanism of action. The experimental results show that the composition can improve the reproductive performance of sows by increasing the total number of piglets and other indicators; meanwhile, the composition can effectively inhibit the replication of PRRSv.

The present application belongs to the technical field of livestock and poultry anti-virus micro-ecological preparation, and particularly relates to a bacillus compound preparation containing grape seed extract and application of the bacillus compound preparation in preparation of a medicine for treating or preventing PRRSV infection. Two strains of bacillus with significant inhibition of PRRSV infection and synergistic effect after being combined with grape seed extract are screened, and the preservation numbers are CCTCC NO: M2026187 and CCTCC NO: M2026186 respectively. The test results show that the compound preparation can significantly reduce the infection activity and proliferation capacity of PRRSV in cells and pigs, effectively reduce the clinical symptoms of pigs caused by PRRSV infection, and relieve the damage degree of lung tissue organs of pigs. The potential synergistic effect between the bacillus compound bacteria and grape seed proanthocyanidin provides a new technical scheme for effective prevention and control of porcine reproductive and respiratory syndrome.

The invention belongs to the technical field of antiviral drugs for livestock and poultry, and discloses application of lysophosphatidylcholine and dihydromyricetin in preparation of drugs for treating or preventing porcine reproductive and respiratory syndrome virus infection. The CAS number of the lysophosphatidylcholine is 17364 to 16 to 8, and the CAS number of the dihydromyricetin is 27200 to 12 to 0. The applicant performs cell level and animal body level anti-infection research on the pharmaceutical composition, and finds that the pharmaceutical composition shows a remarkable anti-PRRSV effect in vivo and in vitro, can reduce proliferation of viruses in a pig body, reduces damage of PRRSV infection to lung tissues and organs, effectively controls adverse effects of PRRSV infection to the body, and has a remarkable anti-PRRSV effect. The clinical disease symptoms of the pigs in the PRRSV infection period can be weakened, and the porcine reproductive and respiratory syndrome can be prevented and controlled.

The present disclosure relates to methods of creating Porcine Reproductive and Respiratory Syndrome virus (PRRSv) resistant pigs by gene silencing. In particular, the methods comprise using tools such as CRISPR / CAS9 to edit genes that either directly encode or encode molecules that are processed into non-coding RNA molecules to induce or modify their silencing activity against CD163 and / or in cells of pigs for the purpose of promoting PRRSv resistance.

The invention belongs to the technical field of animal husbandry epidemic disease prevention and control, and particularly discloses application of scutellaria baicalensis flavone in prevention and treatment of PRRSV (porcine reproductive and respiratory syndrome virus) induced ferroptosis. It is confirmed for the first time that baicalein can play a role in resisting PRRSV by inhibiting ferroptosis in vitro, EC50 is 10.20 [mu] g / mL, a safety window is wide, and the role covers three modes of prevention, co-treatment and treatment; scutellaria baicalensis flavone can improve oxidative stress, iron metabolism disorder and mitochondrial injury of PRRSV (porcine reproductive and respiratory syndrome virus) infected piglet lung tissues in vivo, and the core target is p53. The invention also provides a pig feed containing scutellaria baicalensis flavone / baicalein, and the additive amounts of the scutellaria baicalensis flavone / baicalein are 50-250 mg / kg and 0.1-0.5 g / kg respectively. The invention provides a novel natural traditional Chinese medicine and feed additive for prevention and control of PRRSV, and has the advantages of high safety, definite mechanism, exact in-vivo and in-vitro effects and the like.

The invention discloses application of a PLAC8 promoter methylation promoter in preparation of a product for promoting angiogenesis of a pig placenta, and belongs to the technical field of biological medicines. Experiments prove that PLAC8 as an anti-angiogenesis factor can directly inhibit the angiogenesis function, and DNMT3A can promote placenta vascular development and improve sow farrowing performance by regulating the methylation level of a PLAC8 promoter. In addition, it is determined that daily ration supplementation of methyl donors in the pig placenta vascular development peak period is an important nutrition way for improving DNA methylation of the PLAC8 promoter region. According to the method, the placenta injury mechanism under the adverse pregnancy outcome is directly determined through combination of in-vivo and in-vitro experiments, a theoretical basis is provided for analysis of a regulation strategy for promoting pig placenta angiogenesis and improving sow reproductive performance from epigenetics, and the accuracy and credibility of a result are improved.

The invention belongs to the technical field of livestock breeding, and discloses a preparation method and application of a bacterium-enzyme synergistic fermentation material for improving the reproductive performance of first-born sows and the growth performance of piglets. The invention discloses a fermentation material which is prepared from the following raw materials: dried alfalfa, palm meal, sugar, compound enzyme and compound bacteria, the compound enzyme is composed of cellulase, mannase and xylanase, the invention provides a fermented material, the fermented material is added to a basic feed for feeding first-fetus lactating sows, and the result shows that the daily average feed intake of the lactating sows can be remarkably improved, the backfat loss of the lactating sows is reduced, and the reproductive performance and milk components of the sows are improved; the antioxidant capacity and the immunocompetence of the piglets can be improved, and the inflammation level of piglet bodies can be reduced.

This invention discloses an epitope peptide of the key SRCR5 domain of porcine reproductive and respiratory syndrome virus (PRRSV) CD163, an epitope peptide vaccine, and its applications. The epitope vaccine of this invention exhibits good safety, without safety issues such as virulence reversion, viremia, or recombination with other PRRSV strains. It also demonstrates good immunogenicity; 14 days after secondary immunization, it can induce pigs to produce specific antibodies against the CD163 SRCR5-FP14 epitope with a maximum agglutination titer of 1:16. Immunized pigs can resist challenge from the highly pathogenic PRRSV JAX1 strain. Since all PRRSV subtypes rely on interaction with the CD163 receptor to infect susceptible host cells, and the CD163 molecule is conserved, the epitope vaccine blocking PRRSV infection also provides broad-spectrum protection against multiple subtypes without affecting pig production performance. This novel epitope vaccine has broad potential applications in PRRS prevention and control.

The invention discloses a method for evaluating the reproductive performance of sows. The reproductive performance is at least one of the number of healthy piglets, the number of weak piglets and the weaning litter weight. According to the specific method, genotypes based on an SNP site 1, an SNP site 3, an SNP site 4, an SNP site 7, an SNP site 10, an SNP site 11 and / or an SNP site 12 in a sow genome are detected, so that the reproductive performance of the sow is evaluated or assisted to be evaluated. The method has the advantages of being reliable in result, high in repeatability and high in accuracy, is suitable for stage-by-stage management of reproductive performance of sows in a pig farm and breeding of boars, and has important application prospects and popularization value in modern breeding.

The invention relates to the technical field of livestock breeding, and discloses a breeding system capable of accurately managing the breeding cycle of sows, which comprises an active piezoelectric platform module, a quasi-static fringe electric field detection array and a multi-mode signal processing unit. The active piezoelectric platform module is located at the bottom of the limiting fence, provides vertical mechanical excitation for the sow and monitors the body weight; the quasi-static fringe electric field detection array is located on the side wall, and a deep penetration non-contact detection electric field is constructed by using an active shielding technology. And the multi-mode signal processing unit cooperatively drives the mechanical excitation signal and the electromagnetic carrier signal, and extracts an intermodulation component synchronous with the mechanical frequency from the feedback of the quasi-static fringe field detection array. And the multi-mode signal processing unit calculates a weighted reproductive response index according to a demodulation result, and judges the reproductive cycle state of the sow by using a double-threshold logic tree. On the basis of the mechanical dielectric intermodulation principle, non-intrusive high signal-to-noise ratio monitoring of the oestrus state of the sow is realized by detecting the mechanical compliance change of biological tissues.

This invention discloses an anti-porcine reproductive and respiratory syndrome virus (PRRSV) preparation and its preparation method. The anti-PRRSV preparation comprises traditional Chinese medicine, excipients, and probiotics. The traditional Chinese medicine includes: mustard greens, thistle, bitter melon, and barley sprouts. The excipients include: soybean meal, wheat bran, and molasses. The probiotics include: Bacillus subtilis, Enterococcus faecium, and Saccharomyces cerevisiae. By feeding pigs suffering from PRRSV with the anti-PRRSV preparation of this invention, PRRSV can be antagonized, improving the recovery rate of PRRSV-affected pigs and reducing the mortality rate. The anti-PRRSV preparation of this invention can be used as an antagonist of PRRSV. Furthermore, the anti-PRRSV preparation of this invention can also be used as a feed additive for the prevention or treatment of PRRSV in pigs.

The invention discloses a porcine reproductive and respiratory syndrome virus typing quadruple fluorescent quantitative RT-PCR (reverse transcription-polymerase chain reaction) detection composition and a kit thereof. The detection composition is used for simultaneously detecting four viruses, namely PRRSV-2 (porcine reproductive and respiratory syndrome virus 2), NADC30-like PRRSV (porcine reproductive and respiratory syndrome virus), HP-PRRSV (porcine reproductive and respiratory syndrome virus) and NADC34-like PRRSV; the kit obtained on the basis of the detection composition has good sensitivity, specificity and repeatability, nucleic acids of PRRSV-2, NADC30-like, HP-PRRSV and NADC34-like PRRSV strains can be effectively distinguished, the virus types can be accurately and rapidly identified by analyzing fluorescent quantitative results, the detection period is greatly shortened, and the kit has the advantages of being easy to operate, high in sensitivity, rapid in detection and the like, and has a good application prospect in the detection of PRRSV-2, NADC30-like, HP-PRRSV and NADC34-like PRRSV strains. And a new tool is provided for monitoring the propagation of the PRRSV in the swinery.

To provide pigs that exhibit resistance to porcine reproductive respiratory syndrome virus (PRRSV). [Solution] This disclosure relates to a genetically modified pig in which at least one allele of the SIGLEC-1 gene is inactivated and / or at least one allele of the CD163 gene is inactivated. A genetically modified pig in which both alleles of the SIGLEC-1 gene and / or both alleles of the CD163 gene are inactivated is resistant to porcine reproductive respiratory syndrome virus (PRRSV). Methods for producing such transgenic pigs are also provided.

The invention relates to the technical field of animal nutrition and reproduction, and discloses application of arginine in luteinization of replacement gilts. The arginine is used as a nutritional supplement for regulating and controlling the hormone level of the replacement gilts, the progesterone content can be effectively increased, and corpus luteum generation is promoted. Meanwhile, after the porcine ovarian granular cells are cultured, arginine can up-regulate expression of genes related to synthesis of progesterone and down-regulate expression of genes related to synthesis of estrogen, so that the progesterone is increased, the estrogen is reduced, and the effects of increasing the generation quantity of corpus luteum and maintaining a good pregnancy environment are achieved. According to the invention, 0.5% of arginine is directly added into the pig daily ration, so that the progesterone content in follicular fluid and serum can be remarkably increased, the estrogen content can be reduced, the quantity of small corpus luteum can be increased, and the pregnancy quality and success rate of the replacement gilts after mating can be improved. According to the invention, the problem of hormone level regulation can be solved, and a new nutrition regulation target is provided for increasing the luteum generation of the replacement gilts and improving the reproductive performance of the gilts.

The invention relates to the technical field of livestock breeding biology, and discloses a method for promoting porcine ovary granular cell proliferation, which comprises the following steps: adding a porcine follicular fluid exosome into a porcine ovary granular cell culture system, and acting for a certain time at an effective concentration, so as to improve the proliferation activity of granular cells. After the exosome treatment, the apoptosis rate of granular cells can be reduced, the cell survival rate can be improved, and the granular cells can be promoted to secrete steroid hormones, including the estradiol level and the expression of progesterone synthetase. Cell experiments prove that the follicular fluid exosome can effectively improve the activity of granular cells, promote the process of a cell cycle, inhibit cell apoptosis and remarkably enhance the synthesis capacity of steroid hormones such as estradiol and progesterone, and a new intervention strategy is provided for improving follicular development and reproductive performance of sows.

The invention provides a competition risk-based sow reproduction dynamic early warning regulation and control method and system, and the method comprises the steps: collecting the basic data of a sow at a current observation moment t, extracting a feature vector, calculating the risk ratio of an individual sow to a standard sow based on the feature vector, calculating the accumulated morbidity of each reproduction abnormality event from breeding to the current observation moment t, and carrying out the early warning regulation and control. Calculating the predicted cumulative morbidity of the sow from the current observation moment t to a period of time in the future, determining a risk level according to the predicted cumulative morbidity, generating a control instruction, sending the control instruction to a hardware execution layer through a communication bus, driving the hardware execution layer to execute intervention, and dynamically adjusting the acquisition frequency of a sensor according to the risk level; and body weight and backfat feedback data are obtained after intervention, and if a preset threshold value is not reached, intervention parameters are automatically upgraded, so that closed-loop regulation and control are realized. According to the method, the competing risk model is constructed by taking the abnormal reproduction events as the mutual exclusion events, so that the risk probability is more accurate, and the sow breeding process can be accurately intervened.

The invention belongs to the technical field of antiviral drugs for livestock and poultry, and discloses streptomyces LM-35, a metabolite of the streptomyces LM-35 and application of the streptomyces LM-35 in preparation of drugs for treating or preventing porcine reproductive and respiratory syndrome virus infection. A fermentation supernatant of the streptomyces LM-35 is used for carrying out anti-infection research on PRRSV at a cell level and an animal body level, and the streptomyces LM-35 is found to have remarkable anti-PRRSV activity in vivo and in vitro. The survival rate of the pigs fed with the streptomyces-containing LM-35 fermentation liquor reaches 80%; the adverse effect of PRRSV infection on the body can be effectively controlled, and the method can be used for preventing and controlling the PRRSV.