33results about How to "Improve reproductive performance" patented technology

The invention relates to the technical field of feed additives, in particular to application of 3-indolepropionic acid to improvement of reproductive performance of sows. The 3-indolepropionic acid is added into the feed for the sows in the middle and later periods of pregnancy, is used for improving the reproductive performance of the sows, and has a remarkable effect, so that the use amount of antibiotics for health care can be reduced in the breeding process of the sows. The feed additive prepared from the 3-indolepropionic acid can improve the average litter live piglet weight and average litter healthy piglet number of the sows, improve the lactation ability of the sows and relieve the postpartum lochia condition of the sows.

The invention discloses a strain of lactobacillus reuteri YSLR01 and application of the strain of lactobacillus reuteri YSLR01 in preparation of anti-inflammatory, anti-oxidation, fat-reducing and anti-aging products. According to the invention, a YSLR01 strain with anti-inflammatory, anti-oxidation, fat-reducing and anti-aging effects is separated from excrement of healthy human beings, the strain is preserved in China General Microbiological Culture Collection Center on September 2, 2024, and the preservation number is CGMCC NO.31800. The invention further discloses a preparation method of the YSLR01 strain. Researches show that the YSLR01 strain can significantly reduce secretion of inflammatory factors in a lipopolysaccharide (LPS)-induced RAW264.7 cell inflammation model, improve the oxidation resistance of caenorhabditis elegans, enhance the body length, action ability and reproductive capacity of the caenorhabditis elegans, reduce fat deposition in the body and delay aging of the body.

The present invention discloses a SNP molecular marker related to the reproductive traits of Haimen goats and its application, belonging to the field of biotechnology and livestock breeding. The SNP molecular marker contains a mutation site of Wnt10a g.28944633G>A. The present invention uses the blood genomic DNA of Haimen goats as a template and a method of direct sequencing of PCR products to determine the genotype. Among them, the AA genotype is significantly associated with the number of lambs born by Haimen goats, and can be used as an effective molecular marker to improve the reproductive traits of Haimen goats. The detection method of the present invention is simple, rapid, highly accurate and low-cost, can improve the breeding efficiency of Haimen goats, and has high application value.

The invention discloses a compound grease feed additive for improving breeding quality of channa maculata and application. The method comprises the following steps: (1) preparing experimental feed; the method comprises the following steps: respectively preparing an experimental group ARA 1.5 added with 1.5% of arachidonic acid and an experimental group ARA + PC added with 1.5% of arachidonic acid and 1.5% of phosphatidylcholine; (2) feeding; (3) after the culture experiment is finished, treating and detecting the channa maculata; (4) carrying out an artificial propagation test; (5) carrying out ovarian steroid synthesis related gene expression detection; and (6) carrying out data statistical analysis. By adding arachidonic acid (ARA) and phosphatidylcholine (PC) into the feed, the breeding performance of channa maculata and the quality of fry are improved, and the feed has very important significance for improving the aquatic product efficiency of high-quality fry of channa maculata in aquaculture.

The application relates to application of an olive extract in preparation of a daily ration additive for improving reproduction performance of mammals, wherein 0.05 wt% of the olive extract is added in the daily ration of a pregnant mammal, so as to improve embryo survival rate, live birth number, initial birth litter weight and lactation capacity of the mammal; the application explicitly shows the influence of the olive extract on embryo development and lactation capacity of the mammal, and provides a new technical approach for high-value development of olive resources and nutrition regulation of the mother and the child.

The invention provides a breeding-promoting lactating sow compound feed as well as a preparation method and application thereof. The breeding-promoting lactating sow compound feed comprises the following components in parts by weight: 70-110 parts of a base material, 1-2 parts of calcium salt, 1-3 parts of a functional additive and 1-2 parts of a premix, the functional additive is prepared from the following components in parts by weight: 20 to 40 parts of plant extract, 0.2 to 2 parts of verbascoside, 0.1 to 1 part of geniposide and 0.5 to 2 parts of L-theanine. The compound feed provided by the invention can improve the feed intake of the lactating sows, maintain proper backfat thickness, reduce the weaning loss of the sows and improve the weaning oestrus rate of the sows; excessive free radicals generated by the body can be removed, the balance of a redox system in the body is maintained, oxidative stress is prevented from being generated, the reproductive system of the lactating sow is prevented from being damaged, and the reproductive performance of the sow is improved; and inflammatory factors in the sows can be removed, inflammatory reaction of the sows is avoided, and the reproductive capacity of the lactating sows is improved.

The present application relates to the field of traditional Chinese medicinal materials, in particular to a composition for resisting PRRSv and improving the reproductive performance of sows and application thereof. The present application formulates a composition composed of 10 plant extracts (Angelica sinensis extract, Atractylodes macrocephala Koidz extract, Glycyrrhiza uralensis Fisch extract, Eucommia ulmoides Oliver extract, Eucommia ulmoides leaf extract, Populus davidiana Dode extract, Perilla frutescens leaf extract, Gynostemma pentaphyllum Makino extract, Leonurus japonicus extract and Astragalus membranaceus Bge extract) for sows in late pregnancy, and collects sow blood and placenta samples at the time of delivery, studies the influence of the composition on sow reproduction, and explores the mechanism of action. The experimental results show that the composition can improve the reproductive performance of sows by increasing the total number of piglets and other indicators; meanwhile, the composition can effectively inhibit the replication of PRRSv.

The present application relates to the technical field of livestock data processing, in particular to a livestock and veterinary breeding data analysis system, which comprises: a state transition analysis module collects data of the breeding cycle of female livestock, integrates the data of all female livestock to construct a transition probability matrix, predicts the future state transition path of female livestock according to the transition probability matrix, and generates a female livestock state transition evaluation result. In the present application, by analyzing the data of each state of the breeding cycle of female livestock, the state change path of female livestock at different stages is determined, accurate prediction and quantitative evaluation of the state in the breeding cycle are realized, and the formulation of the breeding plan is more accurate. By evaluating the residence time and transition trend of each stage, the best breeding time is predicted, and the overall breeding efficiency is improved. Based on the similarity analysis of behavior data such as activity frequency, body temperature and number of reproductions, individual female livestock is divided into different behavior groups, and more targeted reproduction management strategies are formulated for different groups.

The invention belongs to the technical field of water treatment, and particularly relates to a biological fluidized bed immobilized microorganism wastewater treatment device and method, the biological fluidized bed immobilized microorganism wastewater treatment device is of a tank body structure, and a biological reaction area is arranged in a tank body; the biological reaction area comprises fixed plates and embedded balls, the fixed plates comprise a first fixed plate and a second fixed plate, and the embedded balls are arranged between the first fixed plate and the second fixed plate; the embedded pellets are prepared from polyvinyl alcohol, sodium alginate, nano modified charcoal and antibiotic degrading bacteria. A biological fluidized bed is creatively combined with an immobilized microorganism technology, so that microorganisms can be stably attached to a carrier, the utilization rate of the microorganisms is increased, and the resistance of the microorganisms to a severe environment is enhanced; according to the biological fluidized bed, microbial carrier particles are fluidized in fluid, so that the contact area between microorganisms and a substrate is increased, and the mass transfer efficiency is improved.

The invention discloses application of quercetin in reduction of the number of resistance genes in soil, solid bacterial fertilizer and a preparation method, and relates to the technical field of preparation of solid bacterial fertilizer and reduction of the number of resistance genes. Quercetin is introduced in the fermentation process of the solid bacterial fertilizer to inhibit propagation and diffusion of resistance genes, and the ecological safety of the bacterial fertilizer is improved on the premise that growth and proliferation of beneficial microorganisms are not affected. The concentration (0.01%-0.05%) of quercetin and the adding mode (added in the first-stage fermentation) are precisely designed, and the inhibition effect of quercetin on beneficial bacteria can be effectively avoided, so that the high efficiency and safety of the bacterial fertilizer are guaranteed.

The invention relates to a sheep BMPR1B gene single nucleotide polymorphism detection primer and application, and belongs to the technical field of genes. The SNP molecular marker corresponding to the primer is located on the sixth chromosome of the BMPR1B gene, and the primer is used for marker-assisted selective breeding of sheep. The invention provides a plurality of SNP molecular marker sites related to sheep breeding traits, and the genotype of each site is judged by a method of taking sheep blood genome DNA as a template and directly sequencing a PCR product. The molecular marker can be used as an effective molecular marker for improving the breeding traits of the sheep, the detection method is simple and rapid, the accuracy is high, the breeding efficiency of the sheep can be improved, the application value is very high, and more ways are provided for screening and researching the breeding traits of the sheep.

The invention discloses a yak breeding method, and belongs to the technical field of livestock breeding. According to the breeding mode, the yaks in the three-month green grass period from June to August are grazed, the yaks in the three-month withering period from September to November are grazed and are fed with concentrated feed in a supplementary mode, and the yaks in the six-month hay period from December to May are bred in a barn feeding mode. The feed has very positive significance in increasing the weight of yaks, increasing the income of herdsmen, realizing balanced feeding and balanced slaughtering of yaks all year round and protecting the ecological environment.

This utility model provides an aquaculture support frame with multiple hanging plates, including a support body, which includes a support frame. The support frame includes two parallel and spaced first rods, which are connected by three second rods. The second rods are spaced along the length of the first rods, and the first and second rods are perpendicularly connected. A float is provided at the connection between the first and second rods, and the float floats on the sea surface. Fixed anchors are connected to both ends of the first rods. Each second rod is connected to an aquaculture plate by a metal rope, the length of which is no more than 1m. The aquaculture plates are arranged from left to right along the arrangement of the second rods as a first aquaculture plate, a second aquaculture plate, and a third aquaculture plate. The first aquaculture plate is a vertically placed aquaculture plate, the second aquaculture plate is a triangularly arranged aquaculture plate, and the third aquaculture plate is a horizontally placed aquaculture plate.

The invention discloses a combined artificial fish reef which structurally comprises a fish reef box, a fish inlet cover and a light-transmitting plate, the lower end of the fish inlet cover is installed in a splicing groove formed in the top of the fish reef box in an inserted mode, and the light-transmitting plate is embedded in a cavity panel arranged on the side edge of the fish reef box. Through active zeolite in the oxygenation box, oxygen is generated in the oxygenation box, the oxygen flows upwards along a filter screen and a flow guide groove and enters the fish reef box to oxygenate fish in the fish reef box, flow blocking is conducted on the interiors of flowing grooves in the two sides of the fish reef box through flow blocking plates, the flow speed in the fish reef box is reduced, and the fish reef box is more stable in fish reef. Therefore, the staying time of the fishes in the fish reef box is prolonged, it is ensured that the fishes can breed in the fish reef box, roes produced by breeding adhere to sea grass planted in the fish reef box, and the effect that the fishes breed in the artificial fish reef is improved.

The invention discloses a method for breeding large-size high-quality young crayfish in early spring. The method comprises the following steps: (1) selecting and cleaning a pond; (2) nutrient enrichment culture of adult shrimps; (3) environment regulation and control; (4) parent screening; and (5) breeding young prawns. According to the method, independently researched and developed pellet feed and hermetia illucens protein source fermented feed are utilized to strengthen parents, gonad development of the crayfish is forcibly promoted by utilizing the environment, and the crayfish parents are promoted to mate and lay eggs in advance; an independently researched and developed hermetia illucens protein source pellet feed is used for cultivating young crayfish and cultivating early spring large-size crayfish fry, and a foundation is laid for staggered breeding of crayfish; according to the method, large-scale supply of large-size high-quality seedlings in spring is achieved, the technical bottlenecks that the seedlings of the premna davidiana are difficult to obtain and adult shrimps are excessively concentrated to appear on the market in the current industry are solved, and healthy and sustainable development of the industry is promoted.

A method for promoting ovarian development of pelteobagrus vachelli belongs to the technical field of fish culture, and is characterized in that in the pelteobagrus vachelli culture process, a functional additive is fed at the ovarian development preparation stage and the ovarian development strengthening stage, and the functional additive is prepared by mixing epigallocatechin gallate and compound unsaturated fatty acid, adding the compound unsaturated fatty acid into the mixture, and stirring to obtain the functional additive for promoting the ovarian development of the pelteobagrus vachelli. Beta-cyclodextrin is firstly adopted for inner layer embedding, then chitosan is adopted for outer layer coating, the microcapsule is prepared, and the composite unsaturated fatty acid is composed of monounsaturated fatty acid and polyunsaturated fatty acid according to the volume ratio of (0.8-1): (1-1.2). EGCG and compound unsaturated fatty acid are compounded and embedded to prepare microcapsules, the microcapsules are added into feed for feeding, ovarian development of pelteobagrus vachelli is effectively promoted, the number of oocytes of pelteobagrus vachelli is increased, meanwhile, the cell proportion of III-stage oocytes is increased, the reproductive capacity of pelteobagrus vachelli is effectively improved, the survival rate of pelteobagrus vachelli is increased, and the survival rate of pelteobagrus vachelli is increased. The egg laying amount, the fertilization rate and the hatching rate are improved.

The invention discloses a dietary fiber raw material as well as a preparation method and application thereof. The preparation method comprises the following steps: step 1, uniformly mixing fresh potato starch residues with fresh bean residues, crushing and mixing the potato bean residues, stirring with water to adjust the moisture of the materials, adding calcium oxide, continuously stirring, standing, carrying out filter pressing, and scattering into small particles; and 2, steam explosion: mixing ethanol according to a solid-liquid ratio of 1: (4-6), uniformly stirring, standing, carrying out solid-liquid separation by using a centrifugal machine, and drying to obtain the dietary fiber raw material. The potato starch residues are fully utilized, nutrient substances in the fresh bean dregs are supplemented, particularly main nutrient substances in the mixed potato bean dregs formed by the potato residues and the bean dregs are converted and reserved, the potato bean dregs are rich in resistant starch and pectin, the treatment process is relatively simple, an enzyme preparation does not need to be added, and the production cost is low. According to the present invention, the dietary fiber 1: 1 bran replacement is blended into the complete ration of the sow to feed, such that the body condition of the sow can be improved, and the reproductive performance of the sow can be improved;

The invention provides application of an ACOT12 gene in regulation and control of bovine sertoli cell proliferation, and belongs to the technical field of biology. According to the invention, the proliferation condition of the bovine sertoli cells is observed by interfering the expression of the ACOT12 gene. Results show that the ACOT12 interference gene can significantly inhibit the proliferation of the bovine sertoli cells, which prompts that the ACOT12 gene has a positive regulation effect in the proliferation of the bovine sertoli cells.

The invention discloses a method for manufacturing a fermentation bed for cattle breeding, which comprises the following steps: S1, activating a strain, namely mixing and activating the strain and a mixture; s2, laying is conducted, specifically, padding and activated strains are evenly mixed, and laying is conducted according to the preset thickness; s3, raising a bed, placing the raising bed after laying the fermentation bed, slowly heating the fermentation bed, and then putting cattle into the fermentation bed; and S4, later-stage maintenance: in the process of finishing the breeding bed and feeding the cattle into the fermentation bed for breeding, maintaining at set intervals. According to the fermentation bed manufactured through the fermentation bed manufacturing method, ecological cattle raising can be achieved, if the fermentation bed is properly maintained, the growth performance of fattening beef cattle can be remarkably improved, the stress death and culling rate of calves can be reduced, the meat quality can be improved, and the good market price can be obtained. In the south, the reproductive capacity of the reproductive cows can also be remarkably improved.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to a pig live piglet number character and application, and relates to the technical field of biology. According to the application, a substance for detecting the SNP sites is applied to preparation of a product for predicting the character of the number of live piglets born by pigs, and the SNP sites comprise the SNP site rs334299237 and the SNP site rs325934988. The SNP sites are related to the character of the live litter size of the pigs, and can be used for predicting the character of the live litter size of the pigs and screening and breeding sows with the character of high live litter size yield.

The invention provides an application of a YTHDC1 gene as a detection target and an application of an SNP molecular marker related to a sheep monoembryonic multilambing character, and belongs to the technical field of molecular markers and genetic breeding. The invention provides an application of a YTHDC1 gene as a detection target. The lambing performance of the sheep to be detected can be determined by quickly typing the YTHDC1 genotype of the newborn sheep, so that the breeding ewes can be conveniently selected and remained. The invention further provides application of the SNP molecular marker related to the sheep single-birth multi-lambing character, based on sheep genome sequence information (Arv3.1), the SNP molecular marker is located on the 84064404bp site on the sixth chromosome of sheep, and A / G polymorphism exists. The SNP molecular marker disclosed by the invention is remarkably related to the single-birth high-lambing-number character of sheep, and individuals with GG-type polymorphic sites can be selected and remained in the production process of the sheep, so that the fecundity of a sheep group is improved.

The invention provides a method for improving the conception rate of meat cows by using rumen bypass linseed oil and choline chloride. In the cow pregnancy stage, rumen bypass linseed oil and choline chloride are supplemented in basic ration at the same time, a suitable nutrition environment is provided for pregnancy recognition and the occurrence of the physiological process of embryo implantation, the rumen bypass linseed oil and the choline chloride can promote each other, embryonic development is effectively promoted, the conception rate after artificial insemination is remarkably increased, and the reproductive performance of meat cows is effectively improved. The method can be widely popularized and applied in production.

This invention discloses a sheep small RNA miR-433-3p and its applications, belonging to the field of animal molecular breeding and reproductive biology technology. The mature nucleotide sequence of miR-433-3p is shown in SEQ ID NO.1. Studies have shown that after transfection of miR-433-3p mimic into sheep follicular granulosa cells, the expression level of miR-433-3p significantly increased, the cellular EdU positivity rate decreased, the cell cycle G0 / G1 phase ratio increased while the S and G2 phase ratios decreased, the apoptosis rate increased, and the expression of proliferation-related genes / proteins and apoptosis-related genes / proteins changed accordingly; transfection of miR-433-3p inhibitor showed the opposite changes. Based on the above results, miR-433-3p can be used to regulate the proliferation and apoptosis of sheep follicular granulosa cells, and can serve as a candidate molecular marker for constructing an evaluation system related to sheep high fertility screening / breeding, providing experimental basis for the subsequent establishment of molecular evaluation indicators for fertility; its mimics, inhibitors, expression vectors or kits containing it can also be used to screen candidate nutritional factors, bioactive factors or reproductive regulatory factors that affect granulosa cell function.

The invention discloses an improved biochemical sewage treatment system which comprises two CASS pools and a moving frame arranged at the tops of the CASS pools, the two ends of the moving frame are controlled to move through a longitudinal moving assembly, a transverse moving vehicle is arranged on the top face of the moving frame, the transverse moving vehicle is controlled to move through a transverse moving assembly, a sludge pump is installed on the transverse moving vehicle, the inlet end of the sludge pump is connected with an inlet pipe, and the outlet end of the sludge pump is connected with an outlet pipe. The other end of the inlet pipe extends into the CASS pool, the outlet end of the sludge pump is connected with a transverse pipe, the other end of the transverse pipe is connected with a telescopic pipe, the other end of the telescopic pipe is connected with an outlet pipe, and the other end of the outlet pipe extends into the adjacent CASS pool. When the CASS pool is overhauled, sludge is transferred through the sludge pump which moves at any time, the sludge pumping range is expanded, manual participation is reduced, the working efficiency is greatly improved, nitrobacteria complementation is facilitated, the sewage treatment efficiency is improved, the environmental protection risk is reduced, and the CASS pool is suitable for large-scale popularization and application. And the sewage enters the anaerobic tank in two directions, so that the reproductive capacity of denitrifying bacteria is improved, and a powerful guarantee is provided for sewage treatment.

The application provides a method for breeding a Paralichthys olivaceus clone improved strain, and the method comprises the following steps: preparing a high-purity Paralichthys olivaceus gynogenesis diploid, preparing a Paralichthys olivaceus double haploid (DH), preparing a double clone hybrid strain, preparing a recombination double haploid (rDH), cultivating the Paralichthys olivaceus recombination double haploid (rDH), and preparing and cultivating the clone improved strain. The Paralichthys olivaceus clone improved strain with fast growth and high reproductive capacity is bred, and the problem of practical application of fish double haploid breeding can be solved.

The invention belongs to the technical field of animal breeding, and particularly relates to application of pentadecanoic acid compounds and / or heptadecanoic acid compounds in improvement of animal reproductive performance. Research finds that the pentadecanoic acid compound and / or the heptadecanoic acid compound can remarkably improve the reproductive capacity of animals, which is specifically embodied in that the number of live piglets and the number of healthy piglets born by maternal animals can be remarkably increased; the delivery backfat thickness and the weaning backfat thickness of the animals are increased; the weaning backfat loss is reduced; the birth average weight and the weaning average weight of the animal offspring are improved. The method effectively improves the reproductive performance of animals, and has important significance and application value in the animal breeding industry.

The invention discloses a miRNA (micro Ribonucleic Acid) marker related to ovarian granular cell proliferation of small tailed han sheep as well as a detection primer and application of the miRNA marker. The marker is novel-m0298-5p, and the sequence of the novel-m0298-5p is as shown in SEQ ID NO. 1. According to the invention, a novel-m0298-5p targeted WNT5A gene is predicted through bioinformatics, and then technologies such as a dual luciferase report system, an overexpression and knock-down test, RT-qPCR, Western blot, CCK-8, EdU and the like are utilized to further verify that the miRNA marker can inhibit the proliferation of ovarian granular cells of small tailed han sheep, so that the miRNA marker can inhibit the proliferation of ovarian granular cells of small tailed han sheep. The provided novel-m0298-5p can be used as a biomarker to be applied to identification of proliferation of ovarian granular cells of the small tailed han sheep.

The invention discloses a glutamine transaminase culture medium as well as a preparation method and a fermentation process thereof, and belongs to the field of microbial fermentation. The glutamine transaminase culture medium is prepared from the following raw materials: 25 to 35 g / L of saccharose, 1 to 3 g / L of sodium nitrate, 0.5 to 1.5 g / L of dipotassium phosphate, 0.3 to 0.6 g / L of magnesium sulfate, 0.3 to 0.6 g / L of potassium chloride, 0.01 to 0.02 g / L of ferrous sulfate, 15 to 25 g / L of agar, 40 to 60 mL / L of wort, 20 to 40 mL / L of jerusalem artichoke juice and the balance of water. The application has the effect of improving the enzyme activity level of culture fermentation of the strain for producing glutamine transaminase.

This invention discloses a method for alleviating cadmium stress on duckweed using ethyl caffeate. With industrial development, cadmium pollution in water bodies seriously threatens the ecosystem and human health, and traditional remediation methods have shortcomings. Although duckweed has the potential for water body remediation, cadmium stress inhibits its growth. This invention, through concentration gradient experiments, determines the maximum Cd concentration that duckweed can withstand. 2+ The optimal concentration of ethyl caffeate for alleviating cadmium stress is 200 μmol, with a concentration of 10 μmol. Adding 200 μmol of ethyl caffeate per 100 ml of cadmium-contaminated water significantly promotes duckweed growth, increases leaf number, fresh weight, and root length, enhances antioxidant enzyme content, reduces MDA content, increases chlorophyll content, and strengthens photosynthesis. It also significantly reduces cadmium accumulation in duckweed, achieving efficient bioremediation of cadmium pollution in water bodies. This method is simple to operate, low in cost, and environmentally friendly, showing broad application prospects in the field of water pollution control.

This invention, entitled "Application of Bifidobacterium animalis subsp. lactis BL-16 and its Astragalus ferment and anti-aging products," belongs to the field of microbial technology. The technical problem to be solved is to provide an anti-aging Bifidobacterium animalis subsp. lactis and its BL-16 fermented Astragalus extract. The key technical point is the application of Bifidobacterium animalis subsp. lactis BL-16 or its fermented Astragalus extract in the preparation of anti-aging compositions. Bifidobacterium animalis subsp. lactis BL-16 and its fermented Astragalus extract can scavenge free radicals in vivo and in vitro and enhance anti-lipid peroxidation capacity, exhibiting good antioxidant and immunomodulatory effects. They can effectively improve survival rate, motility, and reproductive capacity, thus having a good effect on delaying aging.