12results about How to "Improve reproductive performance" patented technology

The invention discloses a compound grease feed additive for improving breeding quality of channa maculata and application. The method comprises the following steps: (1) preparing experimental feed; the method comprises the following steps: respectively preparing an experimental group ARA 1.5 added with 1.5% of arachidonic acid and an experimental group ARA + PC added with 1.5% of arachidonic acid and 1.5% of phosphatidylcholine; (2) feeding; (3) after the culture experiment is finished, treating and detecting the channa maculata; (4) carrying out an artificial propagation test; (5) carrying out ovarian steroid synthesis related gene expression detection; and (6) carrying out data statistical analysis. By adding arachidonic acid (ARA) and phosphatidylcholine (PC) into the feed, the breeding performance of channa maculata and the quality of fry are improved, and the feed has very important significance for improving the aquatic product efficiency of high-quality fry of channa maculata in aquaculture.

The application relates to application of an olive extract in preparation of a daily ration additive for improving reproduction performance of mammals, wherein 0.05 wt% of the olive extract is added in the daily ration of a pregnant mammal, so as to improve embryo survival rate, live birth number, initial birth litter weight and lactation capacity of the mammal; the application explicitly shows the influence of the olive extract on embryo development and lactation capacity of the mammal, and provides a new technical approach for high-value development of olive resources and nutrition regulation of the mother and the child.

The present application relates to the field of traditional Chinese medicinal materials, in particular to a composition for resisting PRRSv and improving the reproductive performance of sows and application thereof. The present application formulates a composition composed of 10 plant extracts (Angelica sinensis extract, Atractylodes macrocephala Koidz extract, Glycyrrhiza uralensis Fisch extract, Eucommia ulmoides Oliver extract, Eucommia ulmoides leaf extract, Populus davidiana Dode extract, Perilla frutescens leaf extract, Gynostemma pentaphyllum Makino extract, Leonurus japonicus extract and Astragalus membranaceus Bge extract) for sows in late pregnancy, and collects sow blood and placenta samples at the time of delivery, studies the influence of the composition on sow reproduction, and explores the mechanism of action. The experimental results show that the composition can improve the reproductive performance of sows by increasing the total number of piglets and other indicators; meanwhile, the composition can effectively inhibit the replication of PRRSv.

The present application relates to the technical field of livestock data processing, in particular to a livestock and veterinary breeding data analysis system, which comprises: a state transition analysis module collects data of the breeding cycle of female livestock, integrates the data of all female livestock to construct a transition probability matrix, predicts the future state transition path of female livestock according to the transition probability matrix, and generates a female livestock state transition evaluation result. In the present application, by analyzing the data of each state of the breeding cycle of female livestock, the state change path of female livestock at different stages is determined, accurate prediction and quantitative evaluation of the state in the breeding cycle are realized, and the formulation of the breeding plan is more accurate. By evaluating the residence time and transition trend of each stage, the best breeding time is predicted, and the overall breeding efficiency is improved. Based on the similarity analysis of behavior data such as activity frequency, body temperature and number of reproductions, individual female livestock is divided into different behavior groups, and more targeted reproduction management strategies are formulated for different groups.

This utility model provides an aquaculture support frame with multiple hanging plates, including a support body, which includes a support frame. The support frame includes two parallel and spaced first rods, which are connected by three second rods. The second rods are spaced along the length of the first rods, and the first and second rods are perpendicularly connected. A float is provided at the connection between the first and second rods, and the float floats on the sea surface. Fixed anchors are connected to both ends of the first rods. Each second rod is connected to an aquaculture plate by a metal rope, the length of which is no more than 1m. The aquaculture plates are arranged from left to right along the arrangement of the second rods as a first aquaculture plate, a second aquaculture plate, and a third aquaculture plate. The first aquaculture plate is a vertically placed aquaculture plate, the second aquaculture plate is a triangularly arranged aquaculture plate, and the third aquaculture plate is a horizontally placed aquaculture plate.

A method for promoting ovarian development of pelteobagrus vachelli belongs to the technical field of fish culture, and is characterized in that in the pelteobagrus vachelli culture process, a functional additive is fed at the ovarian development preparation stage and the ovarian development strengthening stage, and the functional additive is prepared by mixing epigallocatechin gallate and compound unsaturated fatty acid, adding the compound unsaturated fatty acid into the mixture, and stirring to obtain the functional additive for promoting the ovarian development of the pelteobagrus vachelli. Beta-cyclodextrin is firstly adopted for inner layer embedding, then chitosan is adopted for outer layer coating, the microcapsule is prepared, and the composite unsaturated fatty acid is composed of monounsaturated fatty acid and polyunsaturated fatty acid according to the volume ratio of (0.8-1): (1-1.2). EGCG and compound unsaturated fatty acid are compounded and embedded to prepare microcapsules, the microcapsules are added into feed for feeding, ovarian development of pelteobagrus vachelli is effectively promoted, the number of oocytes of pelteobagrus vachelli is increased, meanwhile, the cell proportion of III-stage oocytes is increased, the reproductive capacity of pelteobagrus vachelli is effectively improved, the survival rate of pelteobagrus vachelli is increased, and the survival rate of pelteobagrus vachelli is increased. The egg laying amount, the fertilization rate and the hatching rate are improved.

This invention discloses a sheep small RNA miR-433-3p and its applications, belonging to the field of animal molecular breeding and reproductive biology technology. The mature nucleotide sequence of miR-433-3p is shown in SEQ ID NO.1. Studies have shown that after transfection of miR-433-3p mimic into sheep follicular granulosa cells, the expression level of miR-433-3p significantly increased, the cellular EdU positivity rate decreased, the cell cycle G0 / G1 phase ratio increased while the S and G2 phase ratios decreased, the apoptosis rate increased, and the expression of proliferation-related genes / proteins and apoptosis-related genes / proteins changed accordingly; transfection of miR-433-3p inhibitor showed the opposite changes. Based on the above results, miR-433-3p can be used to regulate the proliferation and apoptosis of sheep follicular granulosa cells, and can serve as a candidate molecular marker for constructing an evaluation system related to sheep high fertility screening / breeding, providing experimental basis for the subsequent establishment of molecular evaluation indicators for fertility; its mimics, inhibitors, expression vectors or kits containing it can also be used to screen candidate nutritional factors, bioactive factors or reproductive regulatory factors that affect granulosa cell function.

The application provides a method for breeding a Paralichthys olivaceus clone improved strain, and the method comprises the following steps: preparing a high-purity Paralichthys olivaceus gynogenesis diploid, preparing a Paralichthys olivaceus double haploid (DH), preparing a double clone hybrid strain, preparing a recombination double haploid (rDH), cultivating the Paralichthys olivaceus recombination double haploid (rDH), and preparing and cultivating the clone improved strain. The Paralichthys olivaceus clone improved strain with fast growth and high reproductive capacity is bred, and the problem of practical application of fish double haploid breeding can be solved.

The invention belongs to the technical field of animal breeding, and particularly relates to application of pentadecanoic acid compounds and / or heptadecanoic acid compounds in improvement of animal reproductive performance. Research finds that the pentadecanoic acid compound and / or the heptadecanoic acid compound can remarkably improve the reproductive capacity of animals, which is specifically embodied in that the number of live piglets and the number of healthy piglets born by maternal animals can be remarkably increased; the delivery backfat thickness and the weaning backfat thickness of the animals are increased; the weaning backfat loss is reduced; the birth average weight and the weaning average weight of the animal offspring are improved. The method effectively improves the reproductive performance of animals, and has important significance and application value in the animal breeding industry.

This invention discloses a method for alleviating cadmium stress on duckweed using ethyl caffeate. With industrial development, cadmium pollution in water bodies seriously threatens the ecosystem and human health, and traditional remediation methods have shortcomings. Although duckweed has the potential for water body remediation, cadmium stress inhibits its growth. This invention, through concentration gradient experiments, determines the maximum Cd concentration that duckweed can withstand. 2+ The optimal concentration of ethyl caffeate for alleviating cadmium stress is 200 μmol, with a concentration of 10 μmol. Adding 200 μmol of ethyl caffeate per 100 ml of cadmium-contaminated water significantly promotes duckweed growth, increases leaf number, fresh weight, and root length, enhances antioxidant enzyme content, reduces MDA content, increases chlorophyll content, and strengthens photosynthesis. It also significantly reduces cadmium accumulation in duckweed, achieving efficient bioremediation of cadmium pollution in water bodies. This method is simple to operate, low in cost, and environmentally friendly, showing broad application prospects in the field of water pollution control.

This invention, entitled "Application of Bifidobacterium animalis subsp. lactis BL-16 and its Astragalus ferment and anti-aging products," belongs to the field of microbial technology. The technical problem to be solved is to provide an anti-aging Bifidobacterium animalis subsp. lactis and its BL-16 fermented Astragalus extract. The key technical point is the application of Bifidobacterium animalis subsp. lactis BL-16 or its fermented Astragalus extract in the preparation of anti-aging compositions. Bifidobacterium animalis subsp. lactis BL-16 and its fermented Astragalus extract can scavenge free radicals in vivo and in vitro and enhance anti-lipid peroxidation capacity, exhibiting good antioxidant and immunomodulatory effects. They can effectively improve survival rate, motility, and reproductive capacity, thus having a good effect on delaying aging.

The invention belongs to the technical field of fish breeding, and particularly relates to a fish seminal plasma exosome, an extraction method and application in fish breeding. The extraction method of the fish seminal plasma exosome comprises the following steps: S1, centrifuging fish seminal fluid to obtain seminal plasma; s2, mixing the seminal plasma with PBS and BPS according to a certain volume ratio, and standing; s3, centrifuging the standing liquid to obtain a precipitate I; s4, centrifuging the precipitate I to obtain exosome precipitate; s5, adding seminal plasma into the exosome precipitate in proportion, mixing and resuspending to obtain a mixed solution I; s6, centrifuging the mixed solution I obtained in the step S5 to obtain supernate rich in exosome particles; and S7, placing the supernatant in an EPF column for centrifugation. The fish seminal plasma exosome can be used for in-vitro fertilization and artificial insemination pretreatment of fish sperms, especially in-vitro fertilization and artificial insemination pretreatment of turbot sperms, and can improve the fertilization success rate, the sperm activation efficiency and the sperm motility.