Belonging to the technical field of animal origin component molecular detection, the invention provides a detection kit for sheep origin component identification and detection of multi-species origin components in products. The kit includes primers shown as SEQ ID No.2&3, a reaction reagent, positive control and blank control. In addition, the kit also includes specific primers of ordinary cattle, buffalo, pigs, equus, rabbits, foxes, dogs, minks, rats, chickens and ducks, and the nucleotide sequences thereof are shown as SEQ ID No.4-25. The kit provided by the invention is employed for analysis to determine whether the sample contains sheep origin components and can effectively distinguish sheep and goats, and whether pigs, bos turus, buffalo, equus, rabbits, foxes, dogs, minks, rats, chickens, ducks and other animal ingredients are mixed. The invention provides an effective, accurate and reliable means for detection of the animal origin components in meat, hides and feed, and the kit and the method have the characteristics of simplicity, fastness, comprehensiveness, strong specificity, low cost, and wide applicability.