Mink heart DNA detection kit and identification method

A detection kit, identification reaction technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the difficulty of quality control of mink heart and its preparations, the identification of traditional Chinese medicinal materials mink heart, and the inability to distinguish between mink heart and mink heart. It can solve problems such as the heart of easily mixed animals, and achieve the effect of reliable detection results and simple identification methods.

Inactive Publication Date: 2009-05-20
BEIHUA UNIV
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no legal identification method for mink heart in the national standard, and there are no literature reports on the composition research and identification of mink heart at ho

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mink heart DNA detection kit and identification method
  • Mink heart DNA detection kit and identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] The present invention will be further described below using examples.

[0030] A detection kit for mink heart DNA, which comprises:

[0031] (a) Identification reaction system: the total system is 100 μL, which contains buffer 10 μL, 12.5 mMdNTP 4-10 μL, 0.1 mM primer 1 and primer 2 1.5-4.0 μL, Taq DNA polymerase 2-10 μL, to be tested Sample DNA 2-5μL, the rest is made up to 100μL with double distilled water;

[0032] (b) Positive control reaction system: the total system is 100 μL, which contains 10 μL buffer, 12.5 mM dNTP 4-10 μL, 0.1 mM primer 1 and primer 2 1.5-4.0 μL, Taq DNA polymerase 2-10 μL, Mink heart DNA 2-5μL, and make up to 100μL with double distilled water;

[0033] (c) Negative control reaction system: the total system is 100 μL, which contains 10 μL buffer, 12.5 mM dNTP 4-10 μL, 0.1 mM primer 1 and primer 2 1.5-4.0 μL, Taq DNA polymerase 2-10 μL, Chicken heart, duck heart, goose heart and rabbit heart DNA were mixed at a ratio of 1:1 to form a mixture...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the Chinese medicine detection technology field and belongs to a mink heart DNA detection kit, comprising buffer solution, 12.5mM dNTP, one type of 0.1mM primer 1 or 2, Taq DNA polymerase, sample DNA to be detected, and the identification reaction system of double-distilled water; containing buffer solution, 12.5mM dNTP, one type of 0.1mM primer 1 or 2, Taq DNA polymerase, mink heart DNA, and the positive control reaction system of the double-distilled water; including buffer solution, 12.5mM dNTP, one type of 0.1mM primer 1 or 2, Taq DNA polymerase, mixture of chicken heart, duck heart, goose heart and rabbit heart DNA according to 1:1, and the negative control reaction system of the double-distilled water. The mink heart DNA identification method comprises steps such as designing mink heart mitochondrial DNA two-pair specific oligonucleotide primer, artificially synthetizing mink heart mitochondrial DNA two-pair specific oligonucleotide primer, fixing reaction procedures and result judgment, and the like; the method can simultaneously and accurately differ the specificity of various animal hearts which are easily mixed with the mink heat; and the identification method has the advantages of simplicity, rapidness, and reliable detection result, etc.

Description

technical field [0001] The invention relates to the technical field of detection of traditional Chinese medicines, in particular to a detection kit and identification method for mink heart DNA. Background technique [0002] Mink heart is a fine and precious medicinal material, and it is the main drug in Lixin Pills, a Chinese patent medicine. Lixin Pill has the effect of invigorating the heart and calming the nerves, and can treat rheumatic heart disease, tachycardia, arrhythmia and heart failure. At present, there is no legal identification method for mink heart in the national standard, and there is no literature report on the composition research and identification of mink heart at home and abroad. The quality control of mink heart and its preparations has brought great difficulties, and it is difficult to satisfy the identification of mink heart, a traditional Chinese medicine. Contents of the invention [0003] The present inventor follows mitochondrial DNA (mtDNA) ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68
Inventor 李明成张丽华王冰梅孙红霞
Owner BEIHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap