51results about How to "Rapid identification method" patented technology

The invention discloses a sugarcane top rot resistance identification method. The method comprises the steps of injecting a well-prepared sugarcane top rot pathogenic bacterium spore suspension onto a growth point of a sugarcane seedling, setting blank sterile water as a reference, culturing the sugarcane seedling for 10 to 15 days after the sugarcane seedling is inoculated, investigating the pathogenic bacterium breeding situation, and classifying the resistance of the sugarcane variety to the sugarcane top rot into an immunity grade, a disease-resisting grade, a medium-resisting grade and an infectious grade according to the disease degree and the disease situation. The sugarcane seedling top rot is identified by adopting an injection inoculation way, the method is closer to the real situation that the sugarcane seedling in the field is infected with the top rot, and the resistance of the sugarcane seedlings of various varieties to the sugarcane top rot can be directly reflected.

The invention relates to a fast and intelligent waste plastic sorting method. The method comprises the steps of scanning the infrared spectrum of a sample with an attenuated total reflection fourier transform infrared spectrograph to obtain an infrared spectrum data matrix, pre-processing the data matrix including standardization and principal component analysis and dimensionality reduction, and carrying out cluster analysis on data with dimensionality reduced by means of the hierarchical clustering method. The result shows that the sample testing method is free of sample preprocessing and high in sample testing speed, and the accuracy of sorting of waste plastic can reach 100% by means of the principal component analysis - hierarchical clustering identification method. Therefore, fast and intelligent waste plastic sorting is achieved by means of attenuated total reflection fourier transform infrared spectrum and chemical mode combined identification.

The invention discloses a wild ginseng and cultivated ginseng multiple PCR test kit, which is characterized by containing: buffer solution, 12.5mM of deoxy-ribonucleoside triphosphate (dNTP), 0.1mM of one of a primer 1 and a primer 2, Taq DNA polymerase, a sample DNA to be tested and a double-distilled water identification reaction system; or the buffer solution, 12.5 mM of dNTP, 0.1 mM of one of the primer 1 and the primer 2, Taq DNA polymerase, wild ginseng and cultivated ginseng DNA 1:1 mixture and a double-distilled water positive reference reaction system; or the buffer solution, 12.5 mM of dNTP, 0.1 mM of one of the primer 1 and the primer 2, Taq DNA polymerase, araliaceae congeneric DNA 1:1 mixture, and a double-distilled water negative reference reaction system. The detection and identification method comprises the steps of designing two pairs of specific oligonucleotide primers of wild ginseng and cultivated ginseng mitochondrion DNAs, designing two pairs of specific oligonucleotide primers of synthetic wild ginseng and cultivated ginseng mitochondrion DNAs, determining a reaction process, determining result and the like. The method can accurately determine the specificity of both the wild ginseng and the cultivated ginseng, and the detection result is reliable.

The invention discloses a molecular marker combination for rapidly identifying different albino tea tree varieties. The molecular marker combination adopts CSR238, CSR 1297 and LG04-06 primer combinations; the invention further discloses a method for rapidly identifying different albino tea tree varieties by using the molecular marker combination and a kit containing the molecular marker. The molecular marker combination can distinguish and identify the albino tea tree varieties on the market, and the identifying method is simple and fast, and can solve the problem of confused names of albino tea tree varieties on the market for a long time; the invention further discloses a finger-print of the albino tea tree varieties; tea selling enterprises can apply the two-dimensional code albino tea tree varieties generated by the invention to mark the product formed by processing different albino tea tree varieties, thus consumers can more conveniently learn about the product information.

The invention discloses a method for fast identifying frankliniella occidentalis. The method is based on the identification results of forms of six kinds of adult thrips such as frankliniella occidentalis and the like for respectively carrying out PCR amplification by using two pairs of frankliniella occidentalis specific primers F.OL1/F.OR and F.OL2/F.OR to establish the method for fast identifying the frankliniella occidentalis. The invention has the advantages of high speed, accuracy and good specificity, provides an effective identification measure for the quarantine pest of the frankliniella occidentalis, and has important meaning for protecting the ecological environment.

The invention relates to the technical field of traditional Chinese medicine, in particular to an American ginseng DNA detection reagent box and an identification method. The American ginseng DNA detection reagent box comprises five parts, namely a sample pretreatment solution, a genome DNA extracting system, a PCR reaction system, a restriction enzyme reaction system and an observation result system. The identification method comprises six steps, namely detection sample pretreatment, genome DNA extraction, PCR primer design and synthesis, PCR reaction establishment, restriction enzyme reaction and result judgment. According to judgment standards, a 100 bp band and a 50 bp band occur simultaneously, the American ginseng is a salable product, and if only one band occurs or no band occurs, the American ginseng is a fake product. The PCR-AFLP identification method has the advantages of being simple, convenient, fast, reliable in detection result and the like, and the specificity of the American ginseng and the fake product can be identified accurately at the same time.

The invention belongs to the technical field of traditional Chinese medicine species identification, and provides a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for identifying dinodon rufozonatum. The sequence of the premier is DK1-CO1:5'-CAA CTA ACC ACA AAG ACA TCG G-3', DK1-CO2:5'-CTT CTG GCT GGC CGA AAA ATC A-3'; and the characteristic DNA base sequence of dinodon rufozonatum is 5'-GTGCAC-3', which can be recognized and cut by restriction endonuclease APaL I. The identification comprises the following steps: extracting the DNA of a sample; using premiers DK1-CO1 and DK1-CO2 to carry out PCR amplification; using restriction endonuclease APaL I to digest the PCR products; carrying out agarose gel electrophoresis to obtain the analysis result; and comparing the analysis result with the PCR-RFLP characteristic result of dinodon rufozonatum to judge whether the provided sample is dinodon rufozonatum or not. The invention also provides a kit for the identification method. The identification is accurate, rapid, and reliable, and is capable of effectively identifying dinodon rufozonatum from other snakes.

The invention discloses a method for identifying lamb meat and adult mutton, which comprises the following steps of extracting metabonomics data of a sample to be detected, and carrying out chemometrics analysis on the detection data to extract characteristic information, analyzing through the chemometrics, establishing an analysis model to screen difference markers, and using the screened characteristic marker to identify the sample to be detected. A reliable and rapid identification method is provided for distinguishing the lamb meat from the adult mutton, the method is convenient, sensitiveand accurate, and the rapid identification on the mutton can be achieved by screening the difference marker through the analysis model.

The invention discloses a set of real time-loop-mediated isothermal amplification (RT-LAMP) primers for differentiating canine distemper virus (CDV) wild strains from vaccine strains and application of the primers. The RT-LAMP primers comprise a pair of outer primers and a pair of inner primers, wherein the sequences of the outer primers are shown as SEQ ID NO:1 and SEQ ID NO:2 respectively; and the sequences of the inner primers are shown as SEQ ID NO:3 and SEQ ID NO:4 respectively. When the primers are used for detecting various genotypes of CDV wild strains according to an RT-LAMP detection method, the detection results are positive; and when the primers are used for detecting canine distemper vaccine strains, canine parvoviruses and other common canine viruses according to the RT-LAMPdetection method, the detection results are negative. Due to application of the primer sequence by the RT-LAMP detection method, the CDV wild strains and the CDV vaccine strains can be differentiatedaccurately; and the invention has the advantages of high specificity, high sensitivity, high repeatability and the like.

The invention belongs to the technical field of traditional Chinese medicine species identification, and provides a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for identifying garter snake. The sequence of the provided PCR amplification primer is DK1-CO1:5'-CAA CTA ACC ACA AAG ACA TCG G-3', DK1-CO2:5'-CTT CTG GGT GGC CGA AAATC A-3'; the characteristic DNA base sequences of garter snake are 5'-ACTAGT-3', which is positioned at the 121st position to 126th position of the COI sequence, and 5'-GGAAACC-3', which is positioned at the 353rd position to the 359th position of the COI sequence; the restriction endonuclease Spe I can identify and cut the characteristic sequence 5'-GGAAACC-3', the restriction endonuclease BstE II cannot identify the characteristic sequence 5'-GGAAACC-3', and the two restriction endonucleases can only cut garter snake double-enzyme into two segments. The identification process comprises the following steps: extracting the DNA of a sample, using primers DK1-CO1 and DK1-CO2 to carry out PCR amplification, purifying the PCR products, using restriction endonucleases Spe I and BstE II to digest the PCR products; carrying out agarose gel electrophoresis to obtain the analysis result, comparing the analysis result with the PCR-RFLP characteristic result of garter snake to determine whether the provided sample is garter snake or not. The invention also provides a kit for the identification method. The identification method is accurate, rapid, and reliable, and can be widely applied to identification of traditional Chinese herbals.

The invention discloses a method for analyzing flavonoid chemical components in walnut flowers. The method comprises the following steps: extracting the medicine walnut flowers by using an ethanol heating reflux method, concentrating, redissolving, carrying out ultrasonic treatment, and filtering to obtain a test solution; detecting the test solution by using an ultrahigh performance liquid chromatography-quadrupole-time of flight mass spectrometry so as to obtain secondary mass spectrometry information of compounds, and analyzing the information about the chemical components in the detectionresult. The method provided by the invention can be used for rapidly and efficiently analyzing the flavonoid compounds in the walnut flowers, thus providing a basis for the research and rapid and accurate identification of effective substances of the walnut flowers and being beneficial to the development and utilization of the walnut flowers.

The origin of a gemstone often governs its value. Traditional jewelry appraisals attempted the recognition of the origin of a gemstone based on criteria of its physical properties include, but not limited to color, refractive indices, and microstructure. However, these criteria, in addition to its inclusions, are generally failed to resolve the locality and origin of a gemstone without doubt. Through careful examination and compilation of Raman spectroscopic data, Argyle pink diamonds can be classified as two types according to their characteristic Raman spectra. The method of the present invention provides sound basis for the rapid determination of the authenticity of the Argyle pink diamonds by Raman spectroscopy.

The invention relates to the technical field of food detection and discloses a multiple PCR detection kit for kangaroos, guinea pigs and sewer rats. The multiple PCR detection kit is characterized bycomprising an animal tissue mitochondria DNA extraction system and an authenticating reaction system (being similar to a positive control solution reaction system and a negative control solution reaction system). An authenticating reaction total system is 50 microliters and contains 5 microliters of a reaction buffer solution, 2-5 microliters of dNTP, 23 microliters of MgCl, 1-5 microliters of TaqDNA polymerase, 2.25 microliters of a kangaroo primer, 3 microliters of a guinea pig primer, 2 microliters of a sewer rat primer, 2-6 microliters of DNA of a to-be-detected sample and the balance ofdouble distilled water. A multiple PCR authentication method for kangaroo meat, guinea pig meat and sewer rat meat comprises the steps of designing three specific oligonucleotide primers by virtue ofspecies specificity of cytochrome b, determining reaction procedures, determining a result, and the like; and the specificities of three rat meat can be simultaneously distinguished; and the method has the advantages of rapidness, reliable detection result and the like.

The invention belongs to the technical field of animal reproduction, and in particular relates to the field of early embryo sex determination of transgenic dairy cattle. The invention discloses a molecular biology method of early embryo sex determination of transgenic dairy cattle, which comprises the following steps of: incising 4-6 cells from an embryo to be detected by applying a micromanipulation technology, washing the incised cells by using sterile ultra-pure water and putting the cells in a PCR (Polymerase Chain Reaction) tube; adding a proper amount of the sterile ultra-pure water, boiling and placing in trash ice; cooling, centrifuging at a low temperature, and taking a supernatant liquor to perform PCR reaction; detecting PCR amplification products by using agarose gel electrophoresis so as to obtain an electrophoretogram; observing the electrophoretogram, determining the embryo as a bull when a 131bp stripe and a 250bp stripe are simultaneously existent in the electrophoretogram, and determining the embryo as a cow when only a 250bp stripe is existent in the electrophoretogram.

The invention relates to a seedling stage identification method of almond fragrance black tea resources. The method comprises the following steps: (1) extracting aroma substances in fresh tea leaves by using a headspace solid-phase microextraction method: after freeze-drying and crushing a fresh leaf sample, adding water of 90-100 DEG C, soaking at 40-70 DEG C, and inserting an extraction head into a space above the liquid level for aroma adsorption; (2) after the adsorption is finished, inserting an extraction head into a gas chromatography sample inlet for desorption, carrying out gas chromatography separation, carrying out mass spectrometry detection, and analyzing data; and (3) determining the relative percentage content of benzaldehyde in the aroma substances. According to the method,any fresh tea leaves can be detected, whether the black tea product has the aroma characteristics of almond aroma or not can be judged without tea trial-production property study, and a rapid and accurate identification method is provided for seedling stage identification of almond aroma black tea resources.

The invention discloses a molecular beacon probe for rapid detection of rifampin-resistant mycobacterium tuberculosis and a detection method thereof. The molecular beacon probe comprises Beacon 1, Beacon 2, Beacon 3, Beacon 4 and Beacon 5. All 81 sites of a rifampin-resistant rpoB gene are covered by the five probes, so that the rifampin-resistant mycobacterium tuberculosis can be detected. The molecular beacon probe has high detection sensitivity and specificity, and can rapidly and specifically detect the rifampicin-resistant mycobacterium tuberculosis. The invention also discloses a detection method of the molecular beacon probe for rapid detection of the rifampin-resistant mycobacterium tuberculosis.

The invention discloses a method for quickly identifying the specificity of a transgenic herbicide-resistant soybean ZH10-6 transformant based on a multiplex PCR method. The method comprises the following steps of designing and synthesizing a specific primer by using G2-epsps and GAT exogenous insertion gene sequences of the transgenic herbicide-resistant soybean ZH10-6, and constructing a transformant specificity identification multiplex PCR reaction system through primer concentration optimization combination. Four pairs of transformant specific sequences are designed aiming at a unique expression mode (two repeated G2-epsps genes and one GAT gene are inserted to form two expression cassettes and four boundaries) of an exogenous insertion sequence of the transgenic herbicide-resistant soybean ZH10-6, and a soybean endogenous Lectin gene is introduced to construct a quintuple PCR reaction system. And an economic, efficient, accurate and reliable high-throughput technical means is provided for specific identification of the transgenic transformant based on the exogenous target gene.