Application of cabbage type rape floral leaf mutant
A technology of Brassica napus and rapeseed, which is applied in the fields of application, botany equipment and methods, plant gene improvement, etc., can solve the problems of long cycle and achieve the effects of saving labor and time, effectively identifying, and ensuring production quality
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Embodiment 1
[0036] The technical solution of the present invention will be further described in detail below in conjunction with specific embodiments and accompanying drawings. Example 1 Breeding of Homozygous Two-type Lines of Mosaic and Leaf Recessive Genic Sterility in Brassica napus
[0037] see Figure 4 , taking the sterile line 20118A of the homozygous two-type line of recessive genic sterility as the female parent, crossed with the Brassica napus mosaic mutant as the male parent, and used the Linbao line M-6029 to test the sterile line at the same time , the offspring of the test cross were all sterile, indicating that the genotype of the selected individual plant was aabbRfRf.
[0038] The mosaic fertile plant AABBRfRfDD and the hybrid offspring of the sterile plant aabbRfRfdd are semi-mosaic fertile, whose genotype is AaBbRfRfDd, and the F1 generation is self-bagged, and the harvested seeds are sown in the field in autumn, and the field observation is carried out during the flo...
Embodiment 2
[0039] Example 2 Breeding of Mosaic Temporary Line of Brassica napus and Production of Total Sterile Line
[0040] see Figure 4 , using the mosaic sterile plant aabbRfRfDD as the female parent, crossed with the Linbao line M-6029, all the hybrid offspring are sterile plants, the genotype is aabbRfrfDd, half-mosaic shape, the semi-mosaic sterile line and Linbao Line M-6029 was backcrossed, there were 46 semi-mosaic fertile plants aabbrfrfDd in 214 backcross populations, and 3 of them were selected for bagging self-crossing, and 21 mosaic fertile plants were obtained in one of the self-crossed offspring testcross one of them with the mosaic sterile plant, and the offspring of the testcross are all mosaic sterile, indicating that the genotype of the mosaic fertile plant is aabbrfrfDD, that is, the Mosaic Linbao line of Brassica napus.
[0041] The mosaic recessive genic male sterile homozygous two-type line was planted in the female parent row, and the mosaic leaf temporary ins...
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