Application of cabbage type rape floral leaf mutant

A technology of Brassica napus and rapeseed, which is applied in the fields of application, botany equipment and methods, plant gene improvement, etc., can solve the problems of long cycle and achieve the effects of saving labor and time, effectively identifying, and ensuring production quality

Active Publication Date: 2014-07-23
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The other is to use the fertility survey at the flowering stage for identification. This method requires low-temperature vernalization at the seedling stage and can only be carried out after flowering, and the cycle is longer.

Method used

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  • Application of cabbage type rape floral leaf mutant
  • Application of cabbage type rape floral leaf mutant
  • Application of cabbage type rape floral leaf mutant

Examples

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Embodiment 1

[0036] The technical solution of the present invention will be further described in detail below in conjunction with specific embodiments and accompanying drawings. Example 1 Breeding of Homozygous Two-type Lines of Mosaic and Leaf Recessive Genic Sterility in Brassica napus

[0037] see Figure 4 , taking the sterile line 20118A of the homozygous two-type line of recessive genic sterility as the female parent, crossed with the Brassica napus mosaic mutant as the male parent, and used the Linbao line M-6029 to test the sterile line at the same time , the offspring of the test cross were all sterile, indicating that the genotype of the selected individual plant was aabbRfRf.

[0038] The mosaic fertile plant AABBRfRfDD and the hybrid offspring of the sterile plant aabbRfRfdd are semi-mosaic fertile, whose genotype is AaBbRfRfDd, and the F1 generation is self-bagged, and the harvested seeds are sown in the field in autumn, and the field observation is carried out during the flo...

Embodiment 2

[0039] Example 2 Breeding of Mosaic Temporary Line of Brassica napus and Production of Total Sterile Line

[0040] see Figure 4 , using the mosaic sterile plant aabbRfRfDD as the female parent, crossed with the Linbao line M-6029, all the hybrid offspring are sterile plants, the genotype is aabbRfrfDd, half-mosaic shape, the semi-mosaic sterile line and Linbao Line M-6029 was backcrossed, there were 46 semi-mosaic fertile plants aabbrfrfDd in 214 backcross populations, and 3 of them were selected for bagging self-crossing, and 21 mosaic fertile plants were obtained in one of the self-crossed offspring testcross one of them with the mosaic sterile plant, and the offspring of the testcross are all mosaic sterile, indicating that the genotype of the mosaic fertile plant is aabbrfrfDD, that is, the Mosaic Linbao line of Brassica napus.

[0041] The mosaic recessive genic male sterile homozygous two-type line was planted in the female parent row, and the mosaic leaf temporary ins...

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Abstract

The invention relates to an application of a cabbage type rape floral leaf mutant. The cabbage type rape floral leaf mutant is applied by the following steps: hybridizing a sterile plant in a cabbage type rape recessive genic male sterile homozygous two-type line 20118AB serving as a female parent with a cabbage type rape floral leaf mutant serving as a male parent, bagging and performing inbreeding to obtain a floral leaf sterile plant; hybridizing the floral leaf sterile plant with a fertile plant in 20118AB to breed a new homozygous two-type line, namely, a floral leaf recessive genic male sterile homozygous two-type line; hybridizing the floral leaf sterile plant with a temporary maintaining line to obtain a new temporary maintaining line, namely, a floral leaf temporary maintaining line; and hybridizing the cabbage type rape floral leaf recessive genic male sterile homozygous two-type line with the floral leaf temporary maintaining line to produce a cabbage type rape floral leaf complete sterile line. Purity identification and impurity removal at the seedling stage of the complete sterile line are realized by using the character of a floral leaf, the purity of the complete sterile line is increased, the genic male sterile seed production procedure is simplified, and the labor amount of field hybrid seed production is reduced.

Description

technical field [0001] The invention relates to the field of Brassica napus breeding, in particular to the application of a flower leaf mutant of Brassica napus. Background technique [0002] (1) Inheritance of recessive genic male sterile 20118A in Brassica napus [0003] Recessive nuclear male sterility has become an important way to utilize rapeseed heterosis in China because of its complete and stable sterility and wide recovery source. When planting, 50% of the fertile plants in the sterile line need to be manually removed, which limits its wide application, such as double recessive genic male sterile S45A and 117A. [0004] 20118A is a new recessive nuclear male sterile line bred by researcher Sun Chaocai of Shanghai Academy of Agricultural Sciences in 1997. The sterile line has empty anthers, no pollen, bagged self-fertilization, and stable sterility. Not affected by environmental conditions. General genetic studies have proved that 20118A is controlled by 3 pairs ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 杨立勇孙超才周熙荣王伟荣李延莉蒋美艳林金元
Owner SHANGHAI ACAD OF AGRI SCI
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