19 results about "Vernalization" patented technology

The invention relates to the technical field of flower cultivation, and discloses an intelligent flowerpot system for promoting flower growth and flowering, comprising: a flowerpot assembly including an intelligent flowerpot and a temperature control assembly, the intelligent flowerpot being provided with an illumination period control assembly, a moisture control assembly and a nutrition control assembly; the server is connected with the flowerpot assembly, a database and an analysis model are arranged on the server, and the analysis model calculates the temperature condition, the illumination condition, the moisture condition and the nutrition condition required by growth and flowering triggering according to the database and the types of the planted flowers; and the control module is connected with the flowerpot assembly and the server, and accurately regulates and controls the real-time temperature, illumination, moisture and nutrition of flower planting in the intelligent flowerpot through the temperature control assembly, the illumination period control assembly, the moisture control assembly and the nutrition control assembly. According to the intelligent flowerpot, high-precision automatic regulation and control of vernalization, accumulated temperature, illumination, humidity and nutrition of flowers planted in the intelligent flowerpot can be achieved, and flowering is effectively promoted.

This invention relates to a genetic transformation method for Ningxia wolfberry using germinating seeds as explants, belonging to the field of plant genetic transformation technology. The invention mainly includes the following steps: (1) After disinfecting Ningxia wolfberry seeds, inoculate them onto a germination medium, and obtain germinating seeds through vernalization and dark culture treatment; (2) Activate and culture Agrobacterium rhizogenes and Agrobacterium tumefaciens strains carrying target gene expression frames to obtain a bacterial solution for infection; (3) Take the germinating seeds obtained in step (1) and mix them with the bacterial solution obtained in step (2) for infection, then transfer the infected germinating seeds to a co-culture medium or substrate for co-culture; (4) Cultivate the material after co-culture in step (3) to induce root growth, and detect the expression level of the target gene or reporter gene on the roots or plants to obtain transgenic positive plants. This invention has the advantages of simple operation, significantly shortened transformation cycle, and high transformation efficiency.

The invention belongs to the technical field of agricultural biology, and particularly discloses a grape germplasm innovation method based on X-ray gradient mutagenesis and an LD50 precise prediction model, and the method comprises the following steps: selecting an annual grape branch for vernalization treatment, and trimming the annual grape branch into a single-bud stem segment; setting an irradiation time gradient, accurately calculating an LD50 value through a cubic spline interpolation model, and carrying out X-ray irradiation on the single-bud stem segment according to the LD50 value; after irradiation, performing cutting and liquid paraffin sealing, and then performing cuttage, potting and field planting treatment; performing secondary vernalization on mutagenesis plant branches, trimming the mutagenesis plant branches into single-bud stem segments, collecting tender leaves after cutting survival, extracting genome DNA, performing whole genome sequencing, and accurately distinguishing mutagenesis specific variation and natural variation. By establishing a complete technical system of standardized vernalization, gradient irradiation, LD50 accurate calculation and whole genome verification, the problems of inaccurate irradiation parameters, nonstandard material treatment, low mutation screening accuracy and the like in the prior art are solved.

The utility model belongs to the technical field of agricultural planting equipment, and particularly relates to a peony and Chinese herbaceous peony tissue culture seedling electric heating seedling raising device which comprises a device shell and a support, the support is installed in the device shell, a seedling raising device is installed in the device shell, and the seedling raising device comprises a monitoring unit, a spraying unit and an illumination unit. According to the electric heating seedling raising device for the peony and paeonia lactiflora tissue culture seedlings, the monitoring unit is installed, the temperature is accurately controlled to be 0-20 DEG C, and the temperature needed by the peony and paeonia lactiflora tissue culture seedlings in the seedling hardening stage, the rooting stage, the vernalization stage and the abnormal weather is met. The spraying unit is installed and comprises a spraying head, a soil humidity sensor, a ventilation assembly and the like, and the requirements of peony and peony tissue culture seedlings for soil humidity in the germination stage, the leaf expansion stage, the flowering stage and the dormancy stage are met. The illumination unit is installed, the LED lamp tube assembly is utilized, and proper light supplement is provided according to weather conditions, so that the temperature, humidity and illumination required by the growth environment of the peony and paeonia lactiflora tissue culture seedlings are provided, and the peony and paeonia lactiflora tissue culture seedlings grow better.

The invention belongs to the field of plant breeding, and particularly relates to a method for improving the plant height character of wheat. The invention systematically studies the histone modification enzyme responding to vernalization low temperature in wheat, and finds that the overexpression TaELF6 gene can reduce the plant height of wheat, increase the ear length of wheat and improve the heading period of wheat; the TaELF6-4A / 4B / 4D gene is knocked out, so that the wheat plant height can be increased, the tiller number can be increased, and the heading period can be delayed.

This invention discloses a method for industrialized seedling cultivation of Angelica sinensis and a three-dimensional circulating seedling cultivation device, belonging to the field of traditional Chinese medicinal herb seedling technology. It solves the problems of high reliance on manual labor, poor seedling quality, numerous soil-borne diseases, and low seedling efficiency in traditional Angelica sinensis seedling cultivation. The method includes: non-destructive mechanical dewing, cleaning, grading, pelleting, and quality testing of Angelica sinensis seeds; using peat moss, vermiculite, and perlite as base materials, compounded in a 9:3:1 mass ratio to form a seedling substrate; filling the seedling substrate into Angelica sinensis-specific seedling trays; sowing the pelleted seeds; covering with soil and watering; placing the seedling trays in a three-dimensional circulating Angelica sinensis seedling cultivation device for precise environmental control to cultivate seedlings; transferring the seedlings to a cold storage for low-temperature dormancy storage; and transplanting after vernalization. This invention achieves mechanization, intelligentization, and intensification of Angelica sinensis seedling cultivation, reducing the total cost of seedling cultivation and the seedling bolting rate, improving the transplant survival rate, saving land, and is suitable for large-scale industrial application.

The application discloses a kind of strong winter self-incompatible Brassica napus material's breeding method, the breeding method includes the following steps: S1: seed germination;S2: seed germination, cotyledon just transplants when unfolding;S3: seedling stage low temperature treatment, low temperature treatment time is 60-80 days;S4: field transplanting;S5: pollination;S6: harvest seed.The application is by seedling stage low temperature vernalization treatment, advantage is: (1) simple operation.It is only needed to be seedling to 10 leaf period low temperature vernalization treatment, then transplanting field can;(2) greatly shorten breeding cycle.The application is from August to May of next year, significantly shorten the breeding time, improve the breeding efficiency.

The new and distinct cultivar of perennial Salvia plant named ‘Big Sky’ characterized by its large, mounded, vigorous, and winter-hardy habit. The large violet-blue flowers densely arranged in verticils on tall heavily-branched panicles that repeat if deadheaded above a mound of large, rugose, dark-green foliage. The new plant flowers without vernalization. ‘Big Sky’ is useful for landscaping as a specimen plant or en masse.

The invention belongs to the technical field of cultivation and breeding, and particularly relates to a method for regulating vernalization of celery and inducing bolting and flowering, which comprises the following steps: selecting celery seedlings with 4-6 true leaves, and performing vernalization treatment to obtain celery plants; the vernalization treatment is light-dark alternate culture; the duration and temperature conditions are limited; after vernalization treatment, performing bolting and flowering induction on the celery plants; the bolting and flowering induction is light-dark alternate culture; and the duration and temperature conditions are limited. According to the method, the temperature and sunshine duration parameters are accurately regulated and controlled, so that the artificial controllable induction of bolting and flowering processes of different celery varieties is realized, the variety adaptation limitation in the prior art is thoroughly solved, the breeding period of the celery varieties is effectively shortened, and technical support is provided for rapid breeding of good celery varieties.

The present application relates to the technical field of plant growth and development regulation, and relates to a cultivation method for normal flowering and fruiting of winter (semi-winter) plants without low-temperature vernalization. The present application provides a cultivation method for normal flowering and fruiting of winter (semi-winter) plants without low-temperature vernalization, comprising the following steps: (1) sterilization and germination: after sterilization treatment, the plant seeds are germinated and cultivated in darkness to obtain plant seedlings; (2) vernalization-free cultivation: the plant seedlings are irradiated with the following light conditions in the vernalization-free cultivation, the light conditions comprise two or more of 400-500 nm, 600-700 nm and 720-750 nm, the light period adopts 16-22 h light / 2-8 h darkness, the light period temperature ranges from 20-28 DEG C, and the dark period temperature ranges from 18-26 DEG C. The present application actively replaces the sustained low temperature in the flowering induction process of winter plants by using light, a non-temperature environmental factor, thereby realizing exemption from the low-temperature vernalization stage.

The vernalization device for the wheat seeds comprises a box body internally provided with a plurality of layers of partition plates, a box door capable of being opened and closed is installed at the front end of the box body, reflective films are installed on the inner wall of the box body and the lower surfaces of the partition plates, light supplementing lamps are installed on the inner wall of the box door in the vertical direction, and when the box door is in a closed state, the reflective films are arranged on the inner wall of the box door. Light emitted by the light supplementing lamp can be reflected on the seed surface of the partition plate under the action of the reflective film; the wheat seed vernalization device is reasonable in design structure and convenient to use, the vernalization number of wheat seeds can be increased through the design of the multiple layers of partition plates, the illumination time can be reasonably controlled under the condition of uniform illumination through the structure that the light supplementing lamps are matched with the reflective films, and the wheat seed vernalization device has the advantages of being simple in structure and low in cost.

The invention provides a method for regulating and controlling soilless low-temperature vernalization and arrow drawing of cymbidium goeringii, and belongs to the technical field of cymbidium goeringii flowering phase regulation and control. The method comprises the following steps: (1) washing the root system of cymbidium goeringii, and airing to separate out plants with flower buds; (2) loading the plant root with the flower bud into a container containing a culture medium; (3) wrapping with a buffer product; (4) placing the plants in a refrigeration house to complete vernalization; and (5) spraying a plant growth regulator, wrapping the buffer product, and then transferring into an environment with the day temperature of 23-27 DEG C and the night temperature of 13-17 DEG C for flower forcing culture, so that the plant is subjected to arrow drawing, namely the vernalization and arrow drawing process of cymbidium goeringii is completed. According to the method for soilless low-temperature vernalization of the cymbidium goeringii and growth promotion of the arrows, the flowering period of the cymbidium goeringii is manually regulated and controlled, out-of-season or timed flowering of the cymbidium goeringii is achieved, the production cost is reduced, the efficiency is improved, and meanwhile the commodity value and market adaptability of the cymbidium goeringii are improved.

The application discloses application of an anticancer drug to preparation of an accelerated winter wheat breeding drug and relates to the technical field of winter wheat breeding.The application is characterized in that a histone modification enzyme inhibitor or an O-GlcNAcase (OGA) inhibitor is used to treat winter wheat by seed soaking and / or spraying during planting, so that a vernalization regulation flowering pathway can be specifically activated, and expression of winter wheat vernalization genes such as VRN1 is promoted, thereby replacing the low-temperature vernalization treatment process of winter wheat, significantly reducing the rapid breeding cost of winter wheat, simplifying the rapid breeding cultivation mode, and improving the rapid breeding efficiency.The application uses the anticancer drug to treat winter wheat, so that the low-temperature vernalization treatment is not needed in the winter wheat breeding process, and the winter wheat can be planted under a conventional greenhouse (RG) condition (20 DEG C; 16h light: 8h darkness) to realize early heading and flowering, and the breeding period of the winter wheat is significantly shortened.

The present application belongs to the technical field of plant cultivation, and particularly relates to a method for shortening flowering period of non-vernalized Brassica napus seed by using gibberellin treatment. The present application discloses a method for promoting vernalization of winter rape by using gibberellin, which comprises the following steps: (1) subjecting Brassica napus seed SC4 to constant-temperature light-proof germination treatment in a solution containing gibberellin to obtain white Brassica napus seed SC4; and (2) culturing the white Brassica napus seed SC4 under long-day conditions. Under the long-day light conditions, the flowering period of the non-vernalized Brassica napus seed SC4 is extremely significantly shortened and significantly shortened under the treatment of 0.001 mol / L and 0.002 mol / L gibberellin concentration, respectively.

The invention relates to the technical field of plant growth and development regulation and control, in particular to a cultivation method for normal flowering and fruiting of plants without low-temperature vernalization. The invention provides a cultivation method for normal flowering and fruiting of winter (semi-winter) plants without low-temperature vernalization, which comprises the following steps: (1) disinfecting and germinating: disinfecting plant seeds, and germinating and cultivating in the dark to obtain plant seedlings; (2) non-vernalization cultivation: in the non-vernalization cultivation, the plant seedlings are irradiated under the following illumination conditions: two or more of 400-500 nm, 600-700 nm and 720-750 nm, the light period adopts 16-22 h illumination / 2-8 h darkness, and the temperature range of the light period is 20-28 DEG C; the temperature range of the dark period is 18-26 DEG C. According to the method, illumination which is a non-temperature environmental factor is used for actively replacing continuous low temperature in the winter plant flowering induction process, so that exemption of the low-temperature vernalization stage is realized.

A new and unique cultivar of Lavandula plant named ‘Summer Serenade’ that is medium-sized, dense, mounded, well-branched, upright, winter-hardy, perennial habit. The new plant begins flowering early, is freely-flowering without requiring vernalization, and has flowers of dark lavender with calyxes of moderate violet-blue on densely branched panicles. The new plant has excellent vigor, is resistant to deer and rabbits, and is useful in the landscape and as a cut flower.

The new and distinct hybrid of Heuchera plant named ‘Glazed and Infused’ produced large mounds of large, cordate, moderately-dissected, mostly flat leaf blades of charcoal-black surrounding the veins and heavy silver between the veins. The new plant has upright, branched panicles producing deep rose-pink flowers without vernalization. The new plant is vigorous and produces large clumps with many dense leaves and is useful in the landscape or in containers.

This invention discloses a biostimulant prepared using the aerial microalga *Pilidiocystis* sp. FDA-4 and its application, belonging to the field of agricultural biotechnology and environmental microbiology. The biostimulant is prepared by culturing the aerial microalgae under specific stress conditions and then extracting the active ingredients through crushing. Experiments show that applying it to crop seeds during vernalization significantly improves the crop's salt stress tolerance: in a 5‰ NaCl salt stress environment, the germination potential of *Arabidopsis thaliana* seeds increased to 65.74%, and the taproot length of seedlings more than doubled compared to the salt stress group. The preparation process of this invention is green and efficient, effectively alleviating the inhibitory effect of soil salinization on crop growth, providing a novel solution for saline-alkali land improvement and crop yield increase, and has good application prospects.

The invention belongs to the field of plant biotechnology breeding, and particularly relates to an organogenesis approach-based alfalfa rapid tissue culture regeneration method, which comprises the following steps: disinfecting and sterilizing alfalfa seeds, placing the seeds in a seed culture medium for dark culture and vernalization, and alternately culturing the vernalized seeds in a light environment and a dark environment, enabling the seeds to germinate cotyledons; cutting off cotyledons, transferring the cotyledons into a bud induction culture medium, alternately culturing in a light environment and a dark environment, and inducing to obtain cluster buds; transferring the cluster buds into a bud elongation culture medium, and alternately culturing in a light environment and a dark environment to enable the cluster buds to grow, so as to obtain highly developed cluster buds; and transferring the highly developed cluster buds into a rooting culture medium, alternately culturing in a light environment and a dark environment, and culturing and rooting to obtain a complete plant. According to the regeneration material obtained by the method, the conventional tissue culture period of 6-8 months can be shortened to only 1-2 months, and the tissue culture regeneration time is greatly shortened.