226 results about "Vernalization" patented technology

The invention provides a sexual propagation method for polygonatum zanlanscianense. The method consists of the following steps including 1, seed harvesting and treatment, 2, seed stratification, 3, vernalization, and 4, seeding and seedling culture. The proper condition suitable for the germination and growth of polygonatum zanlanscianense seeds is artificially provided, so that polygonatum zanlanscianense young seedlings are promoted to fast grow, a great number of seeds and seedlings can be produced in a short period, and the capability of commercial production of the polygonatum zanlanscianense seeds and seedlings is reached. The method has the characteristics of short time, fast effect taking and low cost, in addition, the scaled operation can be realized, and the popularization and the application are easy.

The invention relates to a technical method for high-efficient breeding of wheat, which comprises low-temperature vernalization, south breeding and generation adding, shuttle breeding, multi-point combined identification and the like. In particular, the technical method comprises the following steps of: treating the seeds obtained after the strong-winterness wheat blossoms for 15 to 20 days by hydrogen peroxide, washing the seeds by pure water, sprouting the seeds at the room temperature, putting the seedlings growing to 1 to 2 centimeters in an artificial weather room, performing the green-body vernalization of the seedlings for 45 and 50 days at a temperature of between 3 and 5 DEG C and a relative humidity of 70 percent and under a light intensity of 15001x; hardening the seedlings of the winter wheat undergoing the vernalization treatment at the normal temperature for 1 to 2 days, and transporting the seedlings to Yuanmou in Yunnan province; and preparing a rice seedling bed, watering and soaking the rice seedling bed, discharging visible water before transplanting, and transplanting the seedlings in mud at dusk. The disease resistance, stress resistance, adaptability and yieldstability selections of breeding materials are carried out by shuttle breeding and multi-point combined identification and the like. The technical method for breeding provides rich reserve of raw materials for the rapid improvement of the wheat variety, and also lays a sold foundation for demonstration and popularization of a new variety in large areas.

The invention relates to a cultivation method of crops, and particularly relates to a cultivation method of broccolis and the method belongs to the technical field of plant cultivation. According to the invention, on the basis of the existing cultivation technologies, lucifugal low-temperature treatment is carried out on germinated broccoli seeds, so that the green vernalization of the broccolis can be promoted; and on the basis of accelerating each state of the flower bud differentiation, the leafing period is brought forward, benefit is brought to the improvement of photosynthetic products and the aim of promoting the high yield of the broccolis is achieved. The cultivation method has the beneficial effects that simplicity and operability are provided, the mature period of the broccolis can be brought forward by about 3 days, the yield is increased by more than 10% compared with the conventional cultivation, and the comprehensive benefit is higher.

The invention discloses a fresh broad bean vernalizing method, which comprises the following steps: soaking seeds, accelerating buds, vernalizing buds, exercising seedlings, sowing, paving a film, managing in fields, and the like. According to the fresh broad bean vernalizing method disclosed by the invention, the fresh broad bean vernalizing principle is utilized to artificially carry out proper low-temperature vernalization on the seed in the budding period for a certain time, and the growth time is shortened by utilizing conditions of a greenhouse facility to make the blossoming and fruiting ahead of time so as to enhance the gain. Meanwhile, the fresh broad bean vernalizing method also can efficiently prevent harm of insects, such as bruchus rufimanus and the like, and reduce the application amount of the pesticide. The harvesting time of the fresh bean produced by using the fresh broad bean vernalizing method is more than 40 days earlier than the harvesting time of the fresh bean cultured in the open, the output of the bean is enhanced by more than 50%, and the production value is tripled.

The invention discloses a greenhouse watermelon and greenhouse broad bean rotation method of facility resource continuous utilization. Watermelon vines are pulled away and a greenhouse is cleaned after greenhouse watermelons are collected from August to October of the first year. Broad bean seeds are directly seeded on or broad bean seedlings after manual vernalization are transplanted on mulching films on two sides of an original drip irrigation zone. Broad bean straws are buried green and returned to fields from May to June of a next year, soil is activated, and soil organic matter is improved. The greenhouse is sealed in July of the next year. Autumn waxy corns are planted in the beginning of August of the next year, and fresh corns are harvested and come into the market in the last ten-day period of October. The corn straws are buried green and left mulching films in the field are cleaned in November of the next year. Watermelon plant facility and watermelon seedlings bed for the next year are prepared in December of the next year. According to different crop rotation time of the greenhouse watermelons, broad bean seeds are directly seeded or broad bean seedlings after the manual vernalization are transplanted for forcing culture, and therefore time of broad bean fresh pods to market can be shifted to earlier time from January to June.

A method for planting rape bolts for oilseed and vegetable includes: seeding a double-low rape variety of Ningza No.15 or Ningyou No.16 of Brassica napus in facilities such as greenhouses and the like; after low-temperature vernalization of rape, using a plastic film to cover a greenhouse, covering the greenhouse with straw mulch, using artificial lights to assist in illuminating, keeping night temperature to be above 0 DEG C, and increasing growth temperature of rape and prolonging illumination time to promote rape to enter a bolting period in advance; and harvesting rape bolts of two crops when bolts are level, and harvesting no re-growing bolts to harvest rapeseeds. By the method, the greenhouse is used to produce high-quality pollution-free rape bolts, the bolting period of the high-quality pollution-free rape bolts is shifted earlier by 1-2 months as compared with that of rape bolts in a common field, and high-quality fresh vegetables are provided for the market around spring festival. More than 300kg of rape bolts can be produced per mu of the greenhouse, 170-200kg of rapeseeds can be produced, and the economic benefit is evident.

The invention discloses a method for rapidly obtaining a large number of wheat double-haploid homozygous populations. According to the method, based on a wheat and corn double-haploid breeding technology, through the key links of wheat and corn sowing at different stages, haploid induction, transplantation and vernalization, reduplication and transplantation and winter sowing treatment, after wheat young embryos differentiate into haploid seedlings, transplantation, vernalization and haploid plant reduplication are completed in the same year without over-summer treatment, wheat double-haploid seeds can be harvested in the same year, the requirement for sowing once more can be met after the harvest on the perspective of time, a large number of stable new strains of the wheat homozygous populations can be obtained within one year, the requirement for identifying the new strains at the next stage is met, the natural environment is utilized to the maximum degree, and the time of wheat double-haploid breeding is shortened to one year from two to three years. In this way, the wheat breeding speed is increased, the breeding efficiency is improved, the breeding cycle is shortened, and the breeding cost is lowered.

The invention belongs to the field of haploid breeding technology and discloses a wheat double haploid production method by wheat-and-maize distant hybridization. The main technological characteristics of the method comprise steps of planting, induction of haploid embryo, dissection of haploid embryo, respective isolated culture of small embryo and big embryo into haploid plantlet, vernalization,chromosome doubling, transplantation and harvesting. The big embryo is cultured in big embryo culture medium. The small embryo is firstly cultured in small embryo culture medium and then cultured in basal medium. Small embryo culture medium has a higher sugar concentration, a higher osmotic pressure, richer kinds of amino acids, higher vitamin contents and contains trace of growth regulation factors with the germinating and seedling rate of small embryo being over 75%. By low-temperature vernalization, isolated cultured tissue culture seedlings are directly carried out chromosome doubling andplanting with the doubling treatment efficiency being over 89%. The method provided by the invention is adopted to improve seedling rate of haploid embryo and survival rate and success rate of chromosome doubling, simplify wheat breeding program, increase breeding efficiency and shorten breeding cycle, and has apparent effects on accelerating cultivation of fine varieties.

The invention discloses a method for natural generation-adding breeding of ariplain winter wheat. The method comprises the steps of generation-adding time; generation-adding material; low temperature sprouting; vernalization; timely transplantation; shading treatment; later period film covering and warming; and harvesting and sprouting seeding. According to the method, the cost is reduced, the energy consumption is low, a technology is simple and easy to operate, and the method is suitable for popularization and application.

The invention relates to the technical field of economical forest cultivation and rapid propagation and discloses a styrax hypoglauca grafting method with scion stock time separating treatment. The method includes: collecting scion before budding, sealing with wax and performing cold storage, taking stock, grafting scion, sleeving plastic bags for preserving moisture, and timely removing buds of stock when scion and stock bud to guarantee nutritional growth of scion buds. By the method, vernalization time of scion is guaranteed, budding capacity of scion is improved, stock vitality is guaranteed by delayed grafting, and withering of stock is reduced. By sleeving plastic bags for preserving moisture and removing stock buds, grafting survival rate is increased evidently, statistic average survival rate is above 67%, and mass propagation of styrax hypoglauca clone seedling is achieved.

A method for reproducing Jerusalem artichoke culturing seedlings on vernalization bed until the seedling has 15-20 cm in height, cutting the seedling into segments, culturing seedlings with said segments in the culture medium prepared from humas soil, fertilizer, trace elements and macro elements, and transplanting by hole sowing with soil. Its advantage is high survival rate. Said Jerusalem artichoke can be used to prevent soil erosion.

The invention discloses a Xundian county wheat summer planting generation-adding method which belongs to the technical field of wheat summer planting generation adding. Optimal dormancy breaking methods, vernalization conditions, seedtime and field management modes of different winter and spring wheats are researched, thereby a complete set of wheat summer planting technical system is established, theoretical and technical supports are provided for summer planting generation adding of nationwide wheat heredity and breeding and Yunnan province wheat parent and elite seed expanding propagation, the improvement of food security of China is facilitated, and the social harmony and stability and rural economic development are promoted. By means of the method, the wheat summer planting generation adding can be well achieved in Xundian county.

The invention discloses an improved wheat shoot apex transformation method induced by agrobacterium which comprises, (1) subjecting the seeds to vernalization for 20-30 days at 4 deg. C after germination, (2) activating bacillus which contains destination genes, (3) cutting the seedling of suitable size, exposing or damaging growing point positions, (4) dropping the bacillus droplet containing the destination genes onto the seedling incision, (5) recovering seedling growth, transplanting into soil for cultivation, screening antibiotics to obtain transgene strains and filial generation, (6) identifying transgene strain and the filial generation.

The invention discloses a garlic sprout seed cultivation method. According to the garlic sprout seed cultivation method, a mean of tissue section is adopted, garlic sprouts seeds which can be directly sowed are obtained via cultivation in a 120-day vernalization period in each year. The garlic sprouts seed cultivation method specifically includes: 1) primarily selecting seeds; 2) secondly selecting the seeds; 3) growing seedlings; 4) managing seedbeds; 5) managing fields; 6) performing cross pollination; 7) selecting seed producing farms; 8) harvesting; 9) screening and drying. The garlic sprouts seed cultivation method has the advantages that after primary selection and second selection, quality of the cultivated garlic sprouts seeds is improved; by controlling surrounding environments of the seed producing farms, pollination quality of the seeds is guaranteed, and cross pollination is avoided; after 2-3 years of seed production, the garlic sprout seeds which can be directly sowed are obtained, cost is lowered, and economic benefit is increased.

The invention discloses a greenhouse broad bean ultra-early-maturing high-efficiency cultivation method. The harvesting of fresh pods is started from December and is completed till the Labor Day. The method sequentially comprises field management steps of seed soaking, culture of bud seedlings, manual vernalization treatment of bud seedlings, drip irrigation and transplantation under (black) films, erection of sunshade nets, erection of a first layer of thin films, erection of a second layer of thin films, greenhouse internal temperature and humidity regulation and control, etc. The manually vernalized broad bean bud seedlings are transplanted from the end of August to the end of October; by adding covering materials and an under-film drip irrigation system and conducting temperature and humidity regulation and control management, the yield of fresh pods is increased by more than 60 percent relative to the yield of fresh pods cultured in open fields, the applicable planting duration is long and reaches three months, the fresh pod harvesting duration is long and reaches six months, and the production value per mu of freshly eaten broad bean pods per season is more than ten thousand Chinese Yuan; compared with other greenhouse fruits and vegetables, the labor is saved, the cost is reduced, the risk is low, the net benefit is high and the ecological effect is remarkable.

The invention discloses a cultivation method for quickly breeding allium fistulosum. The cultivation method mainly comprises the steps that cultivation at different places is reasonably arranged, allium fistulosum seeds are sown at a cold area which is suitable for allium fistulosum growth at the seedling stage, it is guaranteed that the average stem diameter of the allium fistulosum can reach 1-2 cm after the allium fistulosum seeds are sown for about 90 days, the natural environment when the first frost date approaches at the local place is fully utilized, so that the allium fistulosum is subjected to vernalization smoothly, plants are transported to the area with the temperature being 20-30 DEG C for field planting, the plants can enter in a reproductive growth stage smoothly, the allium fistulosum blooms after field planting is conducted for 30-40 days, and fruitage can be achieved after 60 days; the first alternation of generations of the allium fistulosum can be completed within six months; afterwards, harvested seeds are sown continuously, the temperature is controlled within the range of 10-25 DEG C, it is guaranteed that the average stem diameter of the allium fistulosum can reach 1-2 cm after sowing is conducted for about 90 days, field planting is carried out in the natural environment at the early spring, sudden change of seasons and air temperatures is utilized ingeniously to enable the allium fistulosum to enter in the reproductive growth stage through vernalization, and fruitage is achieved after field planting is conducted for 60-80 days; the rapid two-generation breeding process of the allium fistulosum can be realized in one year. By means of the cultivation method for quickly breeding allium fistulosum, the breeding speed of the allium fistulosum can be greatly increased.

The invention discloses a virus-free rapid propagation technique of edible lily to solve the problem that culture of producing bulbs with virus-free tissue culture bulbs of edible lily is long in cycle. The technique includes planting lily bulbs more than 2.2cm in length, subjecting the bulbs to vernalization by a 4 DEG C refrigerator for 40-45 days, and thereby shortening culture time of the edible lily from 5 years to 3 years. Culture steps sequentially include culture of edible lily stem tip, subculture, strong seedling culture, bulb induction, bulb vernalization, soil culture, and bulb harvesting. The whole production process of the lily bulbs is adjusted and optimized, the culture process of the virus-free bulbs is simplified, the culture time of the virus-free bulbs of the edible lily is shortened greatly, production cost is lowered while bulb quality is improved, market competitiveness of the edible lily bulbs is enhanced, and the technique is applicable to large-scale production of the virus-free bulbs of the edible lily.

The invention relates to the technical field of agricultural technology and biotechnology cross technologies, and particularly discloses a method for rapidly screening low-accumulation heavy metal crop varieties and a device used by the method. The method comprises the steps that the device for rapidly screening the low-accumulation heavy metal crop varieties is manufactured, then crop seeds are subjected to vernalization, the seeds are cultivated by using a nutrient solution after the seeds are sowed in nutrient agar medium of the device, after crops are cultivated to the required period, a heavy metal solution is added to the nutrient solution, the crops are harvested after cultivation, and low-accumulation heavy metal crop variety screening is carried out. The device is made of light-proof PVC boards, so that harmful substances are not prone to be leached out to affect a research result, and a dark condition like that roots of the crops are in soil can be simulated; a centrifuge tube and small holes are simultaneously used for marking, and thus the marking is convenient; and by adopting a water culture mode for cultivation, the nutrient solution can provide nutrients and water, so that the survival rate is high, and clean and complete crop plants, particular the roots, can be obtained rapidly and accurately.

The invention discloses a method for cultivating a new species of super-high-yield wheat. The method includes the steps that (1) mandatory vernalization is conducted on wheat after dormancy breaking and accelerating germination are conducted till the wheat grows to three centimeters in height; (2) corn seedlings grow till the third section of leaf sheath exposes the head; (3) the wheat is cut off from the position of the tillering node through a disinfection blade, and the upper portion of the wheat tillering node serves as cion; (4) a grafting hole is formed in the middle of the second leaf sheath and the third leaf sheath of each corn seedling in a drilled mode; (5) the cion is inserted into the grafting hole; (6) the corn seedlings are transferred to a nutrition cultivation box to be cultivated for 30-35 days and are sprayed with water, and foliage dressing is conducted on the corn seedlings; (7) the corn seedlings are transferred to an artificial intelligent climate chamber, and corn seeds and wheat seeds can be harvested three months later; (8) grafting backcross with the corn is conducted on half the wheat seeds, and sub-secondary-generation seeds are obtained; (9) grafting backcross with the corn is conducted on half the sub-secondary-generation seeds, and sub-third-generation seeds are obtained. According to the method, broken root regeneration of allopolyploid wheat is utilized to cultivate a high-lighting-effect super-high-yield wheat new species, and the wheat new species is made to have better drought resistance and a high lighting effect so as to increase yield of the wheat.

The invention discloses a novel method of using land and greenhouse for anniversary cultivation of fourth-generation winter wheat. Anniversary cultivation of fourth-generation winter wheat is realized by means of the techniques of embryo seedling, land and greenhouse combined on-site generation acceleration, and the like. The method includes: selecting 14-16d embryos to inoculate to an MB culture medium, so that the embryo seedling rate and embryo seedling transplanting survival rate are high; placing embryo seedlings in an illumination incubator for vernalization for 20-25d at the temperature of 0-6 DEG C; explicating the culture time, place and temperature and light conditions of one-year four-generation cultivation of winter wheat, wherein the greenhouse is 25-30 DEG C in winter, night light supplementation is 1000LX, and natural temperature and light are used in other time for generation accelerated cultivation; and transplanting the embryo seedlings to the land in the midmonth of June. The flowering period avoids the great heat period, and embryo seedling cultivation management measures are taken for increasing the embryo seedling transplanting survival rate and ripening rate. In addition, the method is simple, convenient, feasible, low in cost, high in efficiency and easy to popularize, and is a quick and efficient method provided for winter wheat seed breeding.

The invention discloses a high-yield ecological planting method for broad beans and relates to the technical field of broad bean planting. The high-yield ecological planting method comprises the following steps of (1) land selection and preparation; (2) variety selection; (3) seed vernalization; (4) seedling; (5) transplanting; (6) field management; and (7) harvesting. The planting process is ecological and environment-friendly, the yield of the broad beans can be greatly improved, the quality is obviously improved and the high-yield ecological planting method has popularization significance.

The invention relates to a wheat breeding method, in particular to a wheat winter heating type sunlight greenhouse generation adding method includes the following steps: field soil preparation, field sowing, greenhouse soil preparation, greenhouse transplanting, greenhouse management, harvesting, vernalization, re-sowing. The method can adjust the light, the temperature, water and fertilizers which are needed by the wheat, can relieve prosperous growth of the wheat, can prolong the reproductive period of the wheat, facilitates later pollination and fruit bearing; the wheat is high in fruit bearing rate and is excellent in plumpness, and the thousand seed weight of the wheat can be increased by 17-20%; the germination rate of hybrid seeds can be improved by 8%-10%; a temperature sensor, a humidity sensor and a control device are arranged in the greenhouse, real time control and management are facilitated, and the labor cost can be saved.

The invention provides a breeding method of Chinese cabbages resistant to clubroot. The breeding method comprises the following steps: 1) a to-be-transferred parent A and a transfer source parent B are hybridized and F1-generaiton seeds are obtained; 2) young embryos of the F1-generation seeds are germinated and sown and cultured after being vernalized; 3) F1-generation plants and the to-be-transferred parent A are subjected to backcrossing, and BC1-generation seeds are obtained; 4) young embryos of the BC1-generation seeds are germinated and sown and cultured after being vernalized; 5) BC1-generation plants are screened by means of a molecular marker, and a BC1-generation disease-resistant single plant is obtained; 6) the BC1 disease-resistant single plant and the to-be-transferred parent A are subjected to backcrossing, and BC2-generation seeds are obtained; 7) the young embryos of the BC2-generation seeds are taken as a treatment object, germination, vernalization, sowing, screening and backcrossing in the step 4), the step 5) and the step 6) are repeated four times, and BC6-generation seeds are obtained; 8) a BC6-generation plant is self-crossed, a BC6-generation self-crossed progeny is obtained, and a transfer single plant with homozygous clubroot resistance is obtained.

The invention relates to a method for identifying the springness and winterness of wheat varieties and regulating flower synchronization. In December of every year, at the low position of a vegetable greenhouse warm in winter in the east-west direction, the wheat varieties which are to be identified and to be adopted as parents and the springness-winterness comparison wheat varieties of which the springness and winterness are known are planted in the north-south row direction, the row direction is perpendicular to the low edge of the greenhouse, the wheat variety which is late in heading and has a large heading (flowering) time difference on both ends of the same line (the heading-flowering of wheat at ends close to the edge of the greenhouse firstly occurs through vernalization) belongs to the winter variety; the wheat variety which is early in heading and has small heading (flowering) time difference on both ends of the same line (even the heading-flowering of the wheat at both ends occurs in the same period) belongs to the spring variety. Through the influence of the low temperature outside the winter greenhouse to the close temperature in the greenhouse and by virtue of the temperature-difference effect generated by different distances to the edge of the greenhouse, the speed of vernalization and growth of wheat is influenced, the springness and winterness of wheat varieties can be identified, and time of flower synchronization among different varieties is greatly prolonged, the flower synchronization of any two wheat varieties can be realized with the combination of intermission seeding, and the two wheat varieties are mutually cross-fertilized to be utilized as parents.

The invention discloses application of artificial vernalization to yield breeding of wheat and a method of the application. A wheat variety which has good comprehensive characters and relatively high yield level and has a relatively late heading period under an early sowing condition is selected and dry seeds sufficiently absorb moisture until the seeds are germinated and then are treated at a low temperature of 1-2 DEG C for 5-10 days; then the seeds are subjected to early sowing; and finally, a new wheat variety which has relatively high yield level is cultured by carrying out effect evaluation on testing of a seeding stage, a jointing stage, a heading period, a flowering period and a mature period of the wheat, and the yield and the yield structure. According to the application, the artificial vernalization and wheat yield breeding are combined for the first time, and a selection range of a certain type in the wheat yield breeding in a certain region can be expanded, and the difficulty of the wheat yield breeding in the region is reduced; with the adoption of the method, the high-quality wheat varieties with relatively high yield levels are treated and an adapted region range can be expanded; the novel wheat variety with relatively high yield can be easily obtained; when the cultured new wheat variety is applied, the seeds need to be subjected to the artificial vernalization treatment and then are sowed, so that the application can be used as a technical measure for protecting relevant rights and interests of breeders.

The invention discloses a fresh broad bean-taro efficient facility planting method which comprises the steps such as fresh broad bean vernalization, fresh broad bean winter facility heat preservation, taro early-spring greenhouse cultivation and the like. According to the fresh broad bean-taro efficient facility planting method, the time-to-market of fresh broad beans is more than 40 days ahead of broad beans cultured in the open, taros are harvested more than 20 days ahead, the yields of the broad beans and the taros are increased by 5% or above respectively, and the production value per mu in one year is more than 10 thousand yuan.

The invention relates to a method for transforming a sterile line of Chinese kale by utilizing vernalization and generation adding. The method comprises the following steps of: carrying out hybridization by taking a male sterile line of broccoli cytoplast as a female parent and a selfing line of the Chinese kale as a male parent, so as to obtain hybridized seeds; after the hybridized seeds are planted in summer, carrying out vernalization on plants, and meanwhile, selecting the sterile plants by utilizing pollen microscopic examination or single-plant bagging selfing; and taking the selfing line of the Chinese kale as a recurrent parent, carrying out 3-5-generation backcross with the recurrent parent and the sterile plants, so as to obtain the male sterile line of Chinese kale cytoplast. According to the method, the sterile line of the Chinese kale is transformed by utilizing the male sterile line of broccoli cytoplast, biological characters of the sterile line of the Chinese kale are stable, stamens degenerate, petals and pistils develop normally, nectaries develop well, and seed pods develop normally; the sterile line can be subjected to generation adding propagation under a normal growth condition of the plants, the transformation process is accelerated, the procedures are simplified, the cost is saved, and the purity of the Chinese kale can be improved.

The invention provides a sterile sowing method for ginseng seeds, and belongs to the technical field of ginseng tissue culture. The method includes the steps of seed surface disinfection, inoculation pretreatment and inoculation, cold water immersion is used for simulating vernalization, ginseng seed dormancy is broken through ultrasonic treatment and KOH solution immersion treatment, and ginseng seeds are induced to germinate. The sterile sowing method for the ginseng seeds has the advantages of being low in cost and high in germination rate.