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Forcing culture technique of virus-free lily by industrial tissue culture and low-temperature bulb treatment

A technology of lily grouping and technology, applied in cultivation, soilless cultivation, gardening tools/equipment, etc., can solve the problems of easy spread of insect pests and easy infection of viral diseases, reduce production costs, simplify cultivation procedures, and strengthen market competitiveness Effect

Inactive Publication Date: 2012-11-14
安徽徽生源生物科技股份有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Because this traditional breeding method is very susceptible to viral diseases, and the parasitic pests on the scales are also easy to spread

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] Embodiment: the method that improves the edible lily test-tube bulb to take out the rate of the current year.

[0020] Take the edible lily stem tip, start it up, subculture the strong seedlings, and obtain the virus-free edible lily tissue culture seedlings. After being tested as virus-free seedlings, after the strong seedlings are used to induce regeneration of scales, select scales larger than 2.2 cm, and undergo low-temperature After vernalization at 3-5°C for 40-50 days, plant them in the substrate, observe and record the growth of edible lily stems and underground scales in that year.

[0021] The specific method is as follows:

[0022] 1. Take edible lily bulbs, and peel off 0.2mm stem tips under a dissecting microscope as the starting material for propagation.

[0023] 2. Inoculate the micro shoot tip on the culture medium MS+6-BA0.2mg / l+sucrose 3%+agar 7g / l, PH 5.8 culture medium, culture in the dark for 3-4 days, then turn to the light for culture. T...

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PUM

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Abstract

The invention discloses a virus-free rapid propagation technique of edible lily to solve the problem that culture of producing bulbs with virus-free tissue culture bulbs of edible lily is long in cycle. The technique includes planting lily bulbs more than 2.2cm in length, subjecting the bulbs to vernalization by a 4 DEG C refrigerator for 40-45 days, and thereby shortening culture time of the edible lily from 5 years to 3 years. Culture steps sequentially include culture of edible lily stem tip, subculture, strong seedling culture, bulb induction, bulb vernalization, soil culture, and bulb harvesting. The whole production process of the lily bulbs is adjusted and optimized, the culture process of the virus-free bulbs is simplified, the culture time of the virus-free bulbs of the edible lily is shortened greatly, production cost is lowered while bulb quality is improved, market competitiveness of the edible lily bulbs is enhanced, and the technique is applicable to large-scale production of the virus-free bulbs of the edible lily.

Description

technical field [0001] The invention relates to an edible detoxified lily promoting cultivation technology, in particular to the industrialization of edible detoxified lily tissue culture and the low-temperature treatment of bulbs to promote the cultivation technology. Background technique [0002] Edible lily has been cultivated in my country for nearly a thousand years. According to the "Compendium of Materia Medica" records, lily has the effect of "relaxing the mind, strengthening the mind, and nourishing the five internal organs". Among them, the bulbs of lily are rich in protein, starch, fat and alkaloids, etc., and have high nutritional value. And other effects, so it has been regarded as a good nourishing food. Because edible lily is full of treasures, while the demand in the domestic market is increasing, European and American markets and Southeast Asian countries are also gradually realizing its value. The conventional lily propagation method is generally smal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 何鹏飞何声斌张承妹李琴李传涛陈凤肖建梅陈敏敏陈权
Owner 安徽徽生源生物科技股份有限公司
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