2results about How to "Simplify the breeding process" patented technology

The invention discloses a vegetable breeding method, particularly relates to a green Chinese onion double haploid breeding method, and belongs to the technical field of biotechnology and double haploid breeding. A green Chinese onion double haploid breeding method comprises the following steps: (1) field planting and sampling; (2) carrying out in-vitro gynogenesis culture to induce the embryoid, and taking MS, 1.0 mg.L <-12 >, 4-D, 0.04 mg.L <-1 > NAA, 2.5 mg.L <-16 >-BA, 10% sucrose and 7g.L <-1 > agar as an induction culture medium; (3) culturing embryos and obtaining regenerated plants; (4) cultivation of green Chinese onion seeds. According to the breeding method, the breeding process is accelerated, the pure line is rapidly obtained, the highly homozygous DH line can be obtained only through one generation, the situation that 4-5 generations of continuous selfing are generally needed for breeding of the pure line in a traditional breeding method is changed, the breeding period is remarkably shortened, and the breeding efficiency is improved.

The present application relates to a kind of special strain for solid liquefaction is used to cultivate Ganoderma lucidum of linden and the method for obtaining spore powder, specifically: the present application discloses a kind of Ganoderma lucidum linden cultivation special strain for solid liquefaction medium and preparation method thereof, the medium is composed of the following weight parts of component: wheat starch 15~20 parts, glucose 1~2 parts, wheat cellulose 45~60 parts, bran 15~25 parts, yeast extract 1~2 parts, beef peptone 1~2 parts, potassium dihydrogen phosphate 1~2 parts, 100 parts of water.The present application also provides the special strain cultivation method for solid liquefaction of Ganoderma lucidum using the above-mentioned medium.The method of the present application can improve the breeding efficiency;The obtained mycelium is strong, and the species can be controlled, reduces the risk of variation.