148results about How to "Simplify the breeding process" patented technology

The invention discloses a method for breeding new corn strain by cross inducing parthenocarpy material, belonging to the crop breeding field. The invention mainly uses the Jigao inductive line No. 3, which can cross inducing with high frequency parthenocarpy to generate autocopulative line, to induce following generation to generate haploid after the corn group or hybrid pollination, then forms stable autocopulative line through natural or artificial duplication with haploid. Compared with normal method, the invention is characterized by the simplified breeding process, shortened time, increased efficiency, and autocopulative line of high purity.

The invention relates to a method for selecting male sterility line of rape cytoplasm, wherein the invention comprises that: selecting wide mustard sterility male, using mustard rape as hold line; crossing and molecule mark to assist the selection, to obtain the mustard rape cytoplasm male sterility line and hold line with stable generation; cultivating said sterility line to obtain usable mustard rape cytoplasm male sterility line.

The invention provides a process for producing the oriental lily sterilized seed globule in the bottle, comprising: choosing explant, sterilizing, inducing culture and getting the sterilized stem tip, then breeding culture, subculture, and producing the sterilized seed globule in the bottle directly. The invention produces the sterilized seed globule in the bottle directly to avoid the problem of long period of ball forming and the accumulation of virus existing in the oriental lily, the seed globule can be planted in the soil when out of the bottle, and the survival rate is high. The breeding procedure of the sterilized seed globule is simplified by modifying and optimizing the whole producing procedure for the lily seed globule, and the breeding time of sterilizing the oriental lily is shortened greatly, the quality of seed globule is improved when the producing cost is decreased, which reinforces market competitive power of the domestic self-breeding seed globule and is suitable for the mass production of the oriental lily sterilized seed globule.

The invention belongs to the technical field of rapeseed breeding, in particular to a method for breeding the self-incompatible two-line hybrid seeds of a brassica napus. The invention is characterized in that: aiming at the problem of being incapable of popularizing the self-incompatible hybrid seeds of the brassica napus in large area on production caused by the defects of the existing breeding method for the self-incompatible hybrid seeds of the brassica napus, the invention proposes a breeding method for the breeding the self-incompatible two-line hybrid seeds of the brassica napus, which comprises the breeding and propagating of the self-incompatible hybrid seeds of the brassica napus; and the breeding and propagating of the self-incompatible restorer line of the brassica napus as well as the breeding of the breeding the self-incompatible two-line hybrid seeds of the brassica napus and a production method for the seeds thereof. The invention provides an effective method for the breeding the self-incompatible hybrid seeds of the brassica napus, is favorable to the large area popularization of the self-incompatible hybrid seeds of the brassica napus and promotes the utilization on the advantages of hybrid seeds of the brassica napus.

The invention discloses lucid ganoderma feed containing a lucid ganoderma product, conventional feed and water. The lucid ganoderma feed has the advantages of mass production, stable quality and low cultivation cost and also can enhance the quality of cultivation animals and avoid the residual of chemical substances such as mildew preventives, antibiotics, hormones and the like.

The invention relates to a method for incubating brachymystax lenok seeds, which belongs to the technical field of aquaculture technology, and solves the problem of high death rate during incubation process of brachymystax lenok seeds and the problem that the incubation technologies for other fish seeds are too complex, not easy to promote, and not suitable for incubating brachymystax lenok seeds. The method comprises the following steps: positioning fertilized eggs into a horizontal tank and incubating the fertilized eggs without light until floating upward; then relaying opening, acclimatizing and feeding the fertilized eggs with compound feed, and simultaneously controlling the environmental factors such as the cultivation density until the seeds reach a weight of 10-12g. By using the method, the survival rate of brachymystax lenok seeds can be improved by above 50 percent. The method has the advantages of environment protection, simple operation, low cost and easy promotion.

The invention discloses a method for cultivating a breeding material with broad spectrum and lasting spike blast resistance, belonging to the technical field of rice molecular breeding. The method is characterized in that with a parent of conventional rice or hybrid rice with large production extension area and excellent comprehensive characters as a recurrent parent, broad-spectrum anti-disease genes, Pigm and Pi54, are polymerized in the breeding material through continuous backcross and convergent cross and with combination of marker-assisted selection; meanwhile, representative strains are selected for performing artificial inoculation identification of spike blast, natural inducedidentification in rice blast serious areas and basic agronomic character evaluation during a total growth period, and an advanced line with excellent spike blast resistance is obtained. The agronomic characters of the advanced line bred through the method are similar to or consistent with those of the recurrent parent, and the broad spectrum and lasting resistance to spike blast is enhanced obviously.

The invention belongs to the field of haploid breeding technology and discloses a wheat double haploid production method by wheat-and-maize distant hybridization. The main technological characteristics of the method comprise steps of planting, induction of haploid embryo, dissection of haploid embryo, respective isolated culture of small embryo and big embryo into haploid plantlet, vernalization,chromosome doubling, transplantation and harvesting. The big embryo is cultured in big embryo culture medium. The small embryo is firstly cultured in small embryo culture medium and then cultured in basal medium. Small embryo culture medium has a higher sugar concentration, a higher osmotic pressure, richer kinds of amino acids, higher vitamin contents and contains trace of growth regulation factors with the germinating and seedling rate of small embryo being over 75%. By low-temperature vernalization, isolated cultured tissue culture seedlings are directly carried out chromosome doubling andplanting with the doubling treatment efficiency being over 89%. The method provided by the invention is adopted to improve seedling rate of haploid embryo and survival rate and success rate of chromosome doubling, simplify wheat breeding program, increase breeding efficiency and shorten breeding cycle, and has apparent effects on accelerating cultivation of fine varieties.

The invention discloses an InDel molecular marker KB2 co-separated with a brassica rapa clubroot resistance gene Crrla, a primer and application thereof. The invention further provides a method for identifying or auxiliarily identifying whether a brassica rapa contains the clubroot resistance gene Crrla or not, and whether the brassica rapa is capable of resisting a clubroot disease or not. The method comprises the steps that a to-be-detected brassica rapa genome serves as a template, the primer provided by the invention is subjected to PCR amplification, and whether the to-be-detected brassica rapa contains the clubroot resistance gene Crrla or not and whether the brassica rapa can be capable of resisting the clubroot disease or not are judged according to the size of an amplification product. The molecular marker is utilized to conduct auxiliary selection on the clubroot resistance brassica rapa, the accuracy is high, the cost is low, and both time and labor are saved.

The invention belongs to the field of rape breeding, in particular to a method for breeding a cabbage rape self-incompatible line. The invention aims at the problem of limiting the large area popularization of self-incompatible hybrids caused by the difficult massive propagation of the cabbage rape self-incompatible line, adopts the methods of auxiliary pollination of NaCl liquid plus honey and system selection to overcome the breeding barrier of the cabbage cole self-incompatible line, adopts the measures of early sowing, thin planting and high fertilizer and the like to culture a large-plant type cabbage rape self-incompatible line with a plurality of branches and adopts the selection methods of quality analysis and compatibility authentication and the like to obtain the protospecies of the cabbage rape self-incompatible line, thus further obtaining the protospecies of the cabbage rape self-incompatible line. The invention provides a simple and effective method for the massive propagation of the cabbage rape self-incompatible line and promotes the utilization of the advantages of the self-incompatible hybrids.

The invention discloses a method for breeding inbred line by using sweet potato special materials. The method comprises a special material selection and isolation method, manpower-assisted inbreeding and inbreeding offspring testcross selection. In the invention, a sweet potato special resource which blooms and fructifies naturally and has wide compatibility is taken as a parent material, a plurality of systems with different genetic backgrounds are established at the same time, the inbreeding is performed strictly and the hybridization is avoided by taking isolation measures; the character of blooming and fructifying naturally and the target economical character are successively selected, so as to breed a inbred line material which has excellent target characters and can bloom and fructify naturally stably in the north area in China, the testcross of the inbred line materials is used for identifying the heterosis and the excellent inbred line is used for crossbreeding.

The invention discloses a method for breeding a corn inbred line by using the hybridizing induction of haploids, and belongs to the field of crop breeding methods. The method comprises the following steps: pollinating basic population or cenospecies according with breeding targets by using JASS31, and selecting aleurone layer top purple and purple-free embryonic tip labeled grains; naturally or artificially doubling the obtained material, and inbreding; and selecting non-separated head progeny rows realizing consistent progenies as a new inbred line. The method has a high haploid inductivity reaching above 13%, and has the advantages of obvious selection labeling, easy screening and high accuracy; and additionally, the JASS31 used in the invention has a moderate height, a long flowering time and a large pollen amount, and natural pollination induction of haploids can be carried out, so the artificial pollination can be avoided, labor saving is realized, and it is suitable for the large scale population operation. The method also has the characteristics of high breeding efficiency, simple operation and fast breeding speed.

The invention discloses a virus-free rapid propagation technique of edible lily to solve the problem that culture of producing bulbs with virus-free tissue culture bulbs of edible lily is long in cycle. The technique includes planting lily bulbs more than 2.2cm in length, subjecting the bulbs to vernalization by a 4 DEG C refrigerator for 40-45 days, and thereby shortening culture time of the edible lily from 5 years to 3 years. Culture steps sequentially include culture of edible lily stem tip, subculture, strong seedling culture, bulb induction, bulb vernalization, soil culture, and bulb harvesting. The whole production process of the lily bulbs is adjusted and optimized, the culture process of the virus-free bulbs is simplified, the culture time of the virus-free bulbs of the edible lily is shortened greatly, production cost is lowered while bulb quality is improved, market competitiveness of the edible lily bulbs is enhanced, and the technique is applicable to large-scale production of the virus-free bulbs of the edible lily.

The invention discloses a creation method of a paddy rice common nuclear sterile line and application. The creation method is characterized a common fertile breeding material is used to create the sterile line, the sterile line is hybridized with an intermediate male parent material, hybrid seeds are sorted according to sorting gene expression characters to obtain a large number of sterile-line seeds which do not contain transgenic components, and the reproduction of the common nuclear sterile line is achieved. The common nuclear sterile line and a restoring line are used to produce the hybridseeds, the breeding procedure is simple, a large amount of backcross transformation time can be saved, the sterile characters of the common nuclear sterile line are fast imported into the fertile material, and the breeding cycle is shortened greatly.

The invention discloses a pyramiding breeding method for a rice blast resisting rice variety with the excellent taste. The method comprises the steps that Wu rice 13 and Guandong 194 are selected to be hybridized and matched; then the generation F1 and Wu rice 15 are subjected to multiple crosses, the generation F2 and the generation F3 are subjected to single plant mixed harvesting, marker-assisted selection of dark endosperm mutant genes and rice blast resisting genes is carried out on the product, the obtained dark endosperm mutant gene type is Wx-mqWx-mq, and the obtained rice blast resisting gene type is a three-gene homozygote of Pi-taPi-ta and Pi-bPi-b; subsequent generations are subjected to yield and agronomic trait selection, and rice blast resisting high-yield rice with stable and uniform agronomic traits and the excellent taste is bred on the generation F7. The rice is high in yield and resistant to rice blast and stripe virus diseases, the flexibility of sticky rice and the elasticity of polished round-grained rice are integrated, the taste and quality are very good, the rice is planted in Huaibei and the central region in Jiangsu province under the condition of high and medium fertility, and wide application prospects are achieved.

The invention discloses an artificial reproduction method of mandarin fish. The method comprises steps as follows: parent fish culturing, parent fish selection, artificial spawning induction and fish fry incubation. Compared with the prior art, the artificial reproduction method has the advantages as follows: at the reinforced aquaculture stage of parent fish, male and female parents of the mandarin fish are bred respectively, the breeding process of the male parent is simplified to a certain extent, the female and male parents are managed differently according to physiological characteristics, the sexual maturity stage of the parents is advanced, the gonadosomatic index of male fish is optimized, the quality of ovum and the ovulation amount are improved, the fertilization rate and the hatching rate are further increased, the labor intensity for artificial reproduction and the reproduction cost are reduced, and large-scale popularization of artificial reproduction of the mandarin fish is facilitated.

The invention relates to a selective breeding and propagating method of a plant, particularly relates to a selective breeding and propagating method of a capsicum, belonging to the technical field of plant breeding. Compared with the existing selective breeding technology of a male sterile line of a capsicum, the method has the advantages that the breeding procedure is simple and convenient, the breeding target character and procedure are controllable, the sterile plant rate and the sterile degree are high, and the breeding efficiency is enhanced. A new approach is developed for capsicum heterosis utilization, selective breeding of a leaf color marked sterile line, rapid purity identification of hybrid F1-generation seeds, and the like.

The invention relates to a breeding for culturing good-taste and brown stripe-resisting rice and belongs to the field of genetic improvement of rice and application of biological technology. The method comprises the following steps of: selecting Wujing 13 and Guangdong 194 for matched hybridization; performing individual mixed harvest in the F2 and F3 generations, and performing the molecular mark auxiliary selection of dark albumen mutant genes and brown stripe-resisting genes at the F4 and F5 generations to obtain the double-gene homozygote, of which the dark albumen mutant genotype is Wx-mqWx-mq and the brown stripe-resisting genotype is Stv-biStv-bi; and performing the selection of yield and agronomic character at following generations until the new variety of high-yield rice, namely Nanjing 5055, with stabile and consistent agronomic character, good taste and brown stripe resistance is cultured in the F7 generation. The variety of rice has the advantages of high yield, brown stripe resistance, the combination of the softness of glutinous rice and the elasticity of non-glutinous rice, excellent taste and quality and wide application prospect in proper plantations of riverside areas and southern part of Jiangsu Province.

The invention discloses a herbicide-resistance-based rape hybrid seed producing method and applications thereof, and relates to the field of plant seed production. The method includes (1) introducing a wide-spectrum type herbicide-resistant gene into a rape male parent line to obtain a herbicide-resistant rape male parent line that is an imidazolidinone-resistant line 12WH381 or a glufosinate-resistant line Z7B10, (2) pollinating a female parent that is sterile after a chemical androcidal action by utilizing pollen of the herbicide-resistant rape male parent line, and harvesting hybrid seeds from male parent plants, and (3) coating the hybrid seeds with a herbicide, or spraying a corresponding herbicide to the hybrid seeds when the hybrid seeds are planted to remove false hybrid seeds at the same time of removing weeds so as to obtain high-purity rape hybrid seeds or group. The applications include (1) hybrid seed production for rape, (2) improvement on rape three-line seed production methods at present, (3) improvement on rape nucleus-sterility two-line seed production methods at present and (4) improvement on rape chemical androcidal seed production systems at present.

The invention relates to a breeding method of maize inbred line S37, and belongs to the technical field of agricultural science. The breeding method comprises the following steps: (1) using Shen 137 as a female parent and MO17 as a male parent for hybridization to obtain an F1 generation of Shen 137 / MO17; (2) inbreeding the F1 generation to obtain an F2 generation of Shen 137 / MO17; (3) using an early-maturing density-resistant material FR3 as a female parent and the F2 generation of Shen 137 / MO17 as a male parent for hybridization to obtain the F1 generation of FR3 / Shen 137 / MO17; (4) selecting excellent individual plants from the F1 generation of FR3 / Shen 137 / MO17 as the female parent, using the early-maturing density-resistant material FR3 as the female parent for backcrossing to obtain the F1 generation of FR3 / FR3 Shen 137 / MO17; (5) continuously inbreeding the F1 generation of FR3 / FR3 Shen 137 / MO17 for six generations to breed the maize inbred line S37. The S37 bred according to the method provided by the invention has the advantages of high combining ability, moderate growth period, good plant type, good density resistance, high disease resistance and wide adaptability, has become one of excellent inbred lines commonly used in our country, and is an important basic material for the improvement of maize inbred lines and the hybridization breeding in the future.

The invention relates to an early-stage domestication and cultivation method of paddlefish seedlings, and relates to the early-stage domestication and cultivation method of fish seedlings. The technical problems that the mortality of paddlefish is high, the operation is complicated, the cost is higher, and the popularization is uneasy according to the traditional fish seedling cultivation method are solved, thereby providing the early-stage domestication and cultivation method of paddlefish seedlings. The method comprises the following steps that larval paddlefish seedlings are cultivated with live baits for 5-6d, and then are domesticated and bred with cultivated a compound feed, and simultaneously the breeding density and other environmental factors are controlled until the seedling weight is up to 1.5 to 2g and the seedling length is up to 4 to 5cm to complete the early-stage domestication and cultivation of paddlefish seedlings. The cultivated seedlings have good quality, and the survival rate can be increased by more than 50%. The method provided by the invention has the advantages of simplicity in operation, low cost, easiness in popularization and the like, and is applied to the field of aquatic product breeding.

The invention discloses a breeding method of temperate and tropical corn selfing line QB446. The breeding method includes: using the F1 generation hybridized by a tropical Suwan1(C11) colony materialand temperate Lancaster material representative line Mo17 as the base material, and using a natural selection method and a manual selection method to mutually improve the two materials and perform section. The method has the advantages that the advantages of the two materials are integrated, and the excellent selfing line which is good in resistance, high in seed output rate and good in quality; the breeding procedure is simple, easy in operation, high in success rate and low in manpower and material source consumption as compared with large colony selection; the QB446 bred by the method is high in combining ability, moderate in growth period, high in seed output rate, good in quality, high in disease resistance, fast in dewatering, many in ear rows, thick in ear, widely applicable and thelike.

The invention discloses a ganoderma lucidum bran feed, which is prepared from the following raw materials in parts by weight: 10-20 parts of soybean, 15-30 parts of corn, 25-40 parts of wheat bran, 30-40 parts of rice bran and 3-6 parts of ganoderma lucidum strain. The feed is obtained by culturing and fermenting liquid ganoderma lucidum, so that the meat quality of the conventional domestic animals is achieved, and rich natural health-care factors such as ganoderma lucidum polysaccharides, karnosin and the like are contained simultaneously; ganoderma lucidum mycelia are rich in various pharmacological ingredients and organic germanium which has an anticancer function, so that the generation of interferon, the growth of interferon control cancel cells can be called, the oxygen carrying capacity of red blood cells is enhanced, metabolism is promoted, the organism immunity is enhanced, and the incidence rate is lowered; chemical substances such as mold preventing agents, antibiotics, hormones and the like are added into a formula, so that residue of toxic and harmful substances is avoided, the culturing process is simple and controllable, the culturing success rate is increased, the culturing period is shortened, and batch production is available; the feed has stable quality and low cost, and is suitable for culturing various animals; and the meat quality of cultured animals are enhanced, and most importantly, human body health is facilitated.

The invention discloses a reproductive technique of an intelligent rice sterile line. The reproductive technique is adopted to achieve the effect that reproduced seeds of the intelligent rice sterile line are not required to be subjected to fluorescent sorting. In the method, the intelligent rice sterile line is used as a maintainer line and is hybridized with the corresponding rice sterile line; pollen grains carrying transgenes of the intelligent rice sterile line are aborted and cannot be fertilized, pollen grains without the transgenes are normally fertilized with female gametes of the sterile line and are fruited, and therefore, the fruited seeds of the sterile line are all genetically-modified sterile line seeds and can be used for producing hybrid rice seeds. In the method, the fluorescent sorting procedure is not required in the intelligent rice sterile line reproductive process, and only the reproduction of the maintainer line requires the fluorescent sorting, and therefore, the reproductive process of the sterile line is simplified, the reproductive efficiency of the sterile line is improved and the seed industry benefit is increased.

The invention discloses a culture medium for tissue culture of dragon fruits. The culture medium not only can be used for culturing subculture plantlets, but also can be used for inducing rooting seedlings, i.e., culture steps are simplified, subculture enrichment culture and rooting induction culture are completed by one step, the production period is greatly shortened, and cost is saved. The invention provides the efficient culture medium for the tissue culture of the dragon fruits so as to solve the problems of long production period and high production cost in the dragon fruit cultivating process.

The invention relates to a microalgae culturing method and a grease producing method. The culturing method includes: adding glucose and an alcohol into a culture medium of microalgae, and culturing under photoautotrophy conditions, wherein the alcohol is methanol and / or ethanol. The culturing method and the grease producing method can be used for large-scale low-cost efficient culturing of the microalgae, guarantee rapid growth of the microalgae and allow the microalgae to have a high grease content.

The invention provides a method for breeding trichopria drosophilae Perkins by drosophila melanogaster replacing drosophila suzukii Matsumura. The method includes the steps: firstly, winding one ringof plastics on the inner wall of an open container with drosophila melanogaster pupae; secondly, feeding the drosophila melanogaster pupae until larvae are pupated on the inner wall of the plastics; finally, inoculating the drosophila melanogaster pupae into the trichopria drosophilae Perkins, and breeding the trichopria drosophilae Perkins. Compared with the prior art, according to the method forbreeding the trichopria drosophilae Perkins by the drosophila melanogaster replacing the drosophila suzukii Matsumura, the drosophila melanogaster pupae are easily acquired as compared drosophila suzukii Matsumura phase pupae, feeding cost is low, and drosophila melanogaster pupae are more simply fed. The drosophila melanogaster replaces the drosophila suzukii Matsumura to breed the trichopria drosophilae Perkins, the progeny emergence rate of parasitic wasps is higher, the intrinsic increase rate of the parasitic wasps is higher, and the parasitic wasps are longer in life.

The invention provides a breeding method for a superstrong cold-resistant high yield and oil content brassia campestris L. variety. A winter nature brassia campestris L. variety is used as female parents, a winter nature brassia campestris L. high yield variety is used as male parents, and F1 generation is obtained through hybridization; a parts of F1 generation are frozen in a low-temperature manual climate room, low-temperature stress is relieved gradually, the parts of F1 generation live through the winter under natural winter conditions, survival cold-resistant single plants are harvested; the rest F1 generation are sown in an evaluation nursery and live through the winter under natural conditions, and single plants living through the winter safely are harvested; the survival cold-resistant single plants are F2 generation; the single plants of the F2 generation are sown in a cold-resistant evaluation nursery and live through the winter under natural conditions, and the single plants living through the winter safely are harvested to obtain F3 generation; F4 generation, F5 generation and F6 generation are sequentially obtained by repeating the process for obtaining the F2 generation; comparison between different strains is carried out from the F6 generation, elite plant strains are selected into the evaluation nursery, and the superstrong cold-resistant high yield and oil content brassia campestris L. variety is obtained. The breeding is simple in breeding process, small in blindness, and high in selection efficiency.

The invention discloses an annual culturing method for marine medaka in a lab. Culturing conditions include: culture seawater is 28-30 in salinity and 4-8mg / L in dissolved oxygen level after subjected to five-stage filtering via a nylon film, biological adsorbent, activated carbon, biochemical filter and ultraviolet sterilization system; the culture water is at 26-30 DEG C; the culture density reaches 0.2-0.5L per fish in the growth period and reaches 0.5-1L per fish in the breeding period; a light cycle includes 14 hours of illumination and 10 hours of no illumination, and illumination is performed from 5:00 to 19:00; the culture male-to-female ratio is 2:3; in terms of feeding frequency and feeding time, the fishes are fed with marine tropical fish feed three times a day, from 7:30 to 8:30, from 11:30 to 12:30 and from 15:30 to 16:30, and are fed with Artemia larvae twice a day, from 9:45 to 10:15 and from 13:45 to 14:15. By the annual culturing method, the defects that breeding conditions are difficult to control, cost is high, continuous egg laying fails, egg laying amount is low, breeding conditions are limited, and the like are overcome.

The invention relates to an acer truncatum bunge rapid seedling method, in particular to an in-vitro one-step rapid seedling method of acer truncatum bunge epicormic branch stem sections, and belongsto the technical field of plant rapid propagation. The method provided by the invention comprises the steps of collecting acer truncatum bunge annual epicormic branches; cutting stem sections with buds to be used as explants; after sterilization, inoculating the explants onto an explant germination culture medium so that leaves spread from bud bodies to obtain aseptic seedlings; putting new stem sections of the aseptic seedlings onto a rooting culture medium for directly rooting and seedling; and then, performing transplantation. Acer truncatum bunge tissue culture seedlings obtained by the method provided by the invention are healthy, strong and has a high rooting rate. The rooting rate can reach 85 percent; the plant root systems are exuberant; the transplanting survival rate is as highas 98 percent or higher; in addition, the operation is simple; the cost is low; and the method belongs to an efficient, simple and convenient acer truncatum bunge in vitro propagation technical methodwith a very high popularization and application value.