Method for breeding corn inbred line by using hybridizing induction of haploids

Abstract

The invention discloses a method for breeding a corn inbred line by using the hybridizing induction of haploids, and belongs to the field of crop breeding methods. The method comprises the following steps: pollinating basic population or cenospecies according with breeding targets by using JASS31, and selecting aleurone layer top purple and purple-free embryonic tip labeled grains; naturally or artificially doubling the obtained material, and inbreding; and selecting non-separated head progeny rows realizing consistent progenies as a new inbred line. The method has a high haploid inductivity reaching above 13%, and has the advantages of obvious selection labeling, easy screening and high accuracy; and additionally, the JASS31 used in the invention has a moderate height, a long flowering time and a large pollen amount, and natural pollination induction of haploids can be carried out, so the artificial pollination can be avoided, labor saving is realized, and it is suitable for the large scale population operation. The method also has the characteristics of high breeding efficiency, simple operation and fast breeding speed.

Description

technical field

[0001] The invention belongs to a method for crop breeding, in particular to a method for breeding maize inbred lines by using hybridization to induce haploid. Background technique

[0002] Breeding is a cyclical and long-term work. It generally takes 10-15 years to breed a variety. Not only is the workload heavy, time-consuming and laborious, but it may also take too long. Finally, the results obtained often do not meet the requirements of the market. Therefore, shortening the breeding time and speeding up the breeding speed have always been the goals that breeders are striving for. For this reason, many methods have been proposed, such as using anthers or pollen to cultivate haploids, and then doubling them into inbred lines can greatly shorten the breeding time. Time was once regarded as a promising method, but practice has proved that it is difficult to be widely used in practice due to the difficulty of cultivating anthers, complicated technology, and l...

Examples

Embodiment 1

[0022] Example 1: Breeding maize inbred lines using Jigaoyu Line 31 to induce haploid hybridization

[0023] Proceed as follows:

[0024](1) In 2010, in the Jilin Provincial Academy of Agricultural Sciences, Jigaoyu Line 31 was used as the male parent to pollinate the foreign hybrid T628394 with high yield and excellent comprehensive traits, and 248 ears were harvested from the female plant, and 42326 grains were obtained after threshing According to the traits of the grain markers, that is, the grains whose top aleurone layer and germ tip are both purple are normal hybrid diploid grains; while the top aleurone layer is purple, the germ tip is colorless, and the germ surface is small, showing a Triangular and deeply depressed marks are the induced parthenocarpic "quasi-haploid" grains of T628394; according to the above marks, a total of 6023 "quasi-haploid" grains were obtained, and the haploid induction rate of the grains = quasi-haploid Haploid kernels / number of hybrid kern...

Embodiment 2

[0028] Example 2: Breeding maize inbred lines using Jigaoyu Line 31 to induce haploid hybridization

[0029] Proceed as follows:

[0030] (1) In 2007, a total of 10 people with the blood of Huang Zao Si were Ji 853, Ji 854, Chang 7-2, Si 287, Jing 24, 773-2, Huang C, Huang Zao Si, Si 444, and Zhe 461. The population synthesized by artificial mixing and pollination of inbred lines (referred to as "Huang Zao Si population"). In 2010, "Huangzao Four Populations" was used as the basic material, and Jigaoyu Line 31 was used as the male parent to pollinate the "Huangzao Four Populations", and 315 spikes on the female plant were harvested, with a total of 53,897 grains. The aleurone layer was purple, the germ tip was not purple, and the germ surface was small, triangular, and deeply depressed, and other marking traits were identified, and 7589 "quasi-haploid" grains were obtained.

[0031] (2) In the spring of 2011, the selected "quasi-haploid" seeds were sown in a single seed in J...

technical field

[0001] The invention belongs to a method for crop breeding, in particular to a method for breeding maize inbred lines by using hybridization to induce haploid. Background technique

[0002] Breeding is a cyclical and long-term work. It generally takes 10-15 years to breed a variety. Not only is the workload heavy, time-consuming and laborious, but it may also take too long. Finally, the results obtained often do not meet the requirements of the market. Therefore, shortening the breeding time and speeding up the breeding speed have always been the goals that breeders are striving for. For this reason, many methods have been proposed, such as using anthers or pollen to cultivate haploids, and then doubling them into inbred lines can greatly shorten the breeding time. Time was once regarded as a promising method, but practice has proved that it is difficult to be widely used in practice due to the difficulty of cultivating anthers, complicated technology, and l...

Examples

Embodiment 1

[0022] Example 1: Breeding maize inbred lines using Jigaoyu Line 31 to induce haploid hybridization

[0023] Proceed as follows:

[0024](1) In 2010, in the Jilin Provincial Academy of Agricultural Sciences, Jigaoyu Line 31 was used as the male parent to pollinate the foreign hybrid T628394 with high yield and excellent comprehensive traits, and 248 ears were harvested from the female plant, and 42326 grains were obtained after threshing According to the traits of the grain markers, that is, the grains whose top aleurone layer and germ tip are both purple are normal hybrid diploid grains; while the top aleurone layer is purple, the germ tip is colorless, and the germ surface is small, showing a Triangular and deeply depressed marks are the induced parthenocarpic "quasi-haploid" grains of T628394; according to the above marks, a total of 6023 "quasi-haploid" grains were obtained, and the haploid induction rate of the grains = quasi-haploid Haploid kernels / number of hybrid kern...

Embodiment 2

[0028] Example 2: Breeding maize inbred lines using Jigaoyu Line 31 to induce haploid hybridization

[0029] Proceed as follows:

[0030] (1) In 2007, a total of 10 people with the blood of Huang Zao Si were Ji 853, Ji 854, Chang 7-2, Si 287, Jing 24, 773-2, Huang C, Huang Zao Si, Si 444, and Zhe 461. The population synthesized by artificial mixing and pollination of inbred lines (referred to as "Huang Zao Si population"). In 2010, "Huangzao Four Populations" was used as the basic material, and Jigaoyu Line 31 was used as the male parent to pollinate the "Huangzao Four Populations", and 315 spikes on the female plant were harvested, with a total of 53,897 grains. The aleurone layer was purple, the germ tip was not purple, and the germ surface was small, triangular, and deeply depressed, and other marking traits were identified, and 7589 "quasi-haploid" grains were obtained.

[0031] (2) In the spring of 2011, the selected "quasi-haploid" seeds were sown in a single seed in J...