50results about How to "High pollen" patented technology

The invention discloses a selective breeding method for a black glutinous maize inbred line, which includes the following steps: (1) Jinnuo No. 8 is chosen as female parent and Suyunuo No. 5 is chosen as male parent for hybridization, so that hybrid ears are obtained; (2) after the seeds of the hybrid ears are planted, Jinnuo No. 8 is used as the male parent for backcross, so that backcrossed ears are obtained; (3) after being planted, the seeds of the backcrossed ears are bagged to be inbred, ears with black cobs and seeds are chosen, each ear is separately threshed, the seeds of each ear are planted in a row, and thereby different ear rows are formed; all the individual plants in each ear row are bagged to be inbred, so that inbred ears are obtained, and the inbred ears in the different ear rows are separately harvested; (4) the ear rows in which both the cobs and seeds of all the inbred ears are black are chosen to be left, the seeds of the inbred ears are planted, and according to stress resistance and qualitative character, the excellent individual plants are chosen to be continuously inbred for three generations, so that the black glutinous maize inbred line is obtained. The black glutinous maize inbred line has the advantages of rich nutrition, unique taste, thin pericarp, high glutinousness, high stress resistance, wide applicability and excellent agronomic character.

The invention relates to a breeding method for a plant, in particular to a breeding method for broccoli, belonging to the technical field of plant cultivation. The breeding method comprises the following steps of: performing continuous selfing and directional selection for at least five generations through collected broccoli material to obtain a self compatible line of the broccoli to serve as a transformation male parent to be hybridized with a cytoplasmic male sterile material; breeding a cytoplasmic male sterile line with superior comprehensive performance and stable genetic character through backcross transformation of five generations; then hybridizing the cytoplasmic male sterile line with stable genetic character with the superior self compatible line of the broccoli; and performing field repeated identification and screening to obtain superior hybridized combined variety. According to the method, an optimal growth and development environment for the broccoli is created though low-temperature treatment of seeds in coordination with related facilities and equipment; double blossom and seed setting are realized in one year; the breeding progress can be quickened; and the seed quality is improved.

The invention discloses a method for breeding a corn inbred line by using the hybridizing induction of haploids, and belongs to the field of crop breeding methods. The method comprises the following steps: pollinating basic population or cenospecies according with breeding targets by using JASS31, and selecting aleurone layer top purple and purple-free embryonic tip labeled grains; naturally or artificially doubling the obtained material, and inbreding; and selecting non-separated head progeny rows realizing consistent progenies as a new inbred line. The method has a high haploid inductivity reaching above 13%, and has the advantages of obvious selection labeling, easy screening and high accuracy; and additionally, the JASS31 used in the invention has a moderate height, a long flowering time and a large pollen amount, and natural pollination induction of haploids can be carried out, so the artificial pollination can be avoided, labor saving is realized, and it is suitable for the large scale population operation. The method also has the characteristics of high breeding efficiency, simple operation and fast breeding speed.

The invention relates to a breeding method of crops, and particularly to a breeding method of broccolini, belonging to the technical field of plant breeding. The breeding method has the advantages that 1. the male sterility line belongs to cytoplasmic male sterility and maternal inheritance, and is stable in sterility, the sterile plant rate and the sterility are 100 percent, nectary is developed, more nectars grow, stigma is normal, flowering and fruiting characters are good, and a seedling does not yellow under the condition of low temperature and can not deteriorate after multi-generation backcross, transform is easy, growth is vigorous, and combining ability is strong; 2. a transformation early generation sterile line is stronger in growth vigor in comparison with a maintainer line and has the advantage of interspecies cross; 3. a later producing shoot of the transformation early generation sterile line is stronger than that of the maintainer line, and thus the transformation early generation sterile line material is early planted by 5 days in comparison with a backcross male parent; 4. F1 is prepared by using the sterile line, the seed yield is high, the seed producing cost is low and the labor is saved; and 5. a new variety prepared by using the sterile line is strong in disease resistance, and is remarkable in production increasing.

The invention discloses a breeding method of a corn selfing line and belongs to the technical field of corn breeding. The breeding method includes: using a corn line NH6249 as a basic material; obtaining the corn selfing line NH6249-1 after selfing of several generations. The corn selfing line NH6249-1 obtained by the breeding method has the advantages of high matchability, high yield, high quality and multi-resistance, is suitable in mechanical production and can be matched and bred to obtain a basic material of new corn varieties having excellent performances like high matchability, high yield, high quality and multi-resistance.

The invention introduces a production method of rice seeds. The production method is characterized by adopting rice variety seeds generated by intermediate indica japonica restorer R900. The intermediate indica and japonica restorer R900 is a high-yielding, high-quality and multi-resistant uniform new rice variety selected by breeding an intermediate material with big warehouse, sufficient sources, high setting percentage and good circularity by utilizing strong hybrid vigour of indica japonica subspecies through broad spectrum broad affinity genes, then selecting multi-resistant seeding head germplasm with polymerized indica japonica stress resistance genes by utilizing high pressure screening and finally a variety of rice which is high in yield, good in quality and uniform in multi-resistant performances can be obtained by inspecting the rice quality.

A process for preparing the hybrid seeds of rape and the vegetables in the mustard family by using the sterile cytoplasm of charlock includes such steps as separating the protoplast from leaf pulp, purifying it, pre-treating, fusing, culturing, discriminating the hybrid, reproduction, selectively culturing the male sterility recovery line of charlock cytoplasm, transferring the male sterility and recovery characters from the charlock cytoplasm to rape or the vegetables in mustard family, and hybridizing between the charlock cytoplasm male sterility line of rape or the said vegetables and the male fetile rape or the said vegetables.

The invention discloses a method for selecting early-maturing yellow skin pepper germplasm and belongs to the technical field of plant breeding. Firstly, an ornamental pepper selfing population is subjected to sowing and seedling raising, and plants with viridescent cotyledon and euphylla are selected from the population in the cotyledon period and the euphylla period; in the flowering period, plants with about 10 initial flower sections, upward pedicels, milk white petals, faint yellow placentas, white styles and purple stripes on style tips are selected, and plants which do not conform to any of the features in the population continue to be removed; in the blooming period, each individual plant is covered with a nylon insect-proof mesh bag so that the plants can conduct selfing and fruiting, or selfing is conducted by means of artificial pollination; in the fruiting period, the colors of young fruits, commercially ripe fruits and physiologically ripe fruits are distinguished, individual plants with yellow young fruits and commercially ripe fruits and red physiologically ripe fruits are selected, and the fruits are harvested and seeds are obtained according to the individual plants; individual-plant selfing populations are sown respectively the next year, selection continues to be conducted according to the features, and if the plants in a certain individual-plant selfing population all conform to the features in all the periods, selection of homozygous early-maturing yellow skin pepper germplasm is completed; if the populations continue to be separated, selection continues to be conducted for 2-3 years according to the features.

A method for selectively culturing three lines of hybrid corn rice with ultra-strong dominance by distant hybridization between paddy rice and corn includes such steps as respective sexual hybridization of the sterile line of hybrid rice with the selfing line and recovery line of corn, and continuous respective backcross of the obtained first hybrid generation with the selfing line and recovery line of corn to obtain the sterile line, maintenance line and recovery line of corn rice, which have ultra-strong dominance, high resistance to stress and high yield.

The invention discloses a propagation method of an oil sunflower Xinkui No. 14 sterile line 1666A. The propagation method comprises the following steps of: under the precondition of spatial isolation that no sunflower planting area exits 5km around an isolation area to be chosen, setting the proportion of sterile lines to maintainer lines to be 6: 2; irrigating once every 7 days before squaring stage of sunflower; and configuring a box of strong bee colony in every 0.2hm<2> of seed farm; and establishing a propagation method of oil sunflower sterile line through pollination. Through isolation propagation, the purity can be ensured, and the propagation yield is also improved; and the propagation method is relatively low in cost.

The present invention relates to a restoring line breeding method of the barley combination hybridized by robust and high-quality ones; the method belongs to the technical field of agricultural crop breeding. The method comprises that the two-rowed barley restoring line is taken as the maternal plant and multi-rowed barley as the paternal plant; individual plants with scattered fringe and good flowering habit in the F2 generation to inbreed for 3 to 5 generations and get the restoring line 238; the restoring line 238 is taken as the maternal plant to hybridize with the multi-rowed barley 35 as the paternal plant; the offspring is backcrossed with the 35 paternal plant for 3 to 5 generations and get the flowering restoring line 03-358 with excellent agriculture features. The fringes of the restoring line 03-358 are scattered which is beneficial to long-term loose powder; the blossom is 2 to 3 days which is beneficial to adjusting the parent plants blossoms to meet; the anther is hung outside in blossom which is beneficial to hybridization. The parent 270A is taken as the maternal plant and the restoring line 03-358 is taken as the paternal plant; the combination is hybrid one of strong advantages; the hybrid F1 generation is two-rowed one; the largest fringe has 33 grains and the dry grain weight is about 45 grams; the stem is hard which is lodging resistant and the advantage of the hybrid is strong.

The present invention relates to a high-yield breeding method of round-grained non-glutinous rice sterile line, belonging to the field of seed production technology. Said high-yield breeding method includes the following measures: defining breeding process, defining breeding field, defining ridge-forming mode of male parentage and female parentage, defining reasonable spacing between rows of male parentage plants and spraying plant growth regulating agent, etc. Said invention can raise setting percentage and can greatly raise yield.

The invention discloses a breeding method of an excellent super sweet corn inbreeding line, and belongs to the technical field of agriculture new variety breeding. The breeding method is characterized in that a subtropical variety resource which is good in stress resistance, wide in applicability and good in quality is selected to carry out hybridization and backcross improvement with inbreeding super sweet corn, and strict selection is carried out on width, resistance, quality and the like of grains; excellent genetic genes sourcing from different source resources are gathered together, and richer sweet corn gene resources are provided for breeding. A super sweet corn inbreeding line 403-11A bred through the invention is semi compact in plant type, moderate in ear position, more tolerant in close planting and strong in heat resisting ability and cold resisting ability; higher general combining ability and specific combining ability effects are obtained, a prepared hybrid variety is high in yield, good in high temperature resistance and low temperature resistance, strong in rain resistance and wide in applicability, and a high-yield and stable-yield variety can be easily bred; the grains are relatively outstanding in quality, appearance properties, seed yield and the like, and the super sweet corn inbreeding line is good in comprehensive properties.

The invention discloses a production method of rice seeds. The method is characterized in that rice seeds produced by indica / japonica intermediate type restoring series R900 are adopted, wherein the indica / japonica intermediate type restoring series R900 are the intermediate materials which are prepared through wide spectrum and wide compability gene according to the high heterosis of indica / japonica subspecies; the intermediate materials are large in capacity, sufficient in source, high in ripening rate, and good in circularity; then the high pressure sieving is carried out to select multi-antibody seedling germplasm with polymerized indica / japonica stress resistance genes; the new rice spicy with high output, high quality and unified multi-antibody performances can be selected by rice inspection.

The invention discloses the cuttage method for the meeting of kiwi fruit in flower period and relates to the plant meeting method in flower period, especially to the kiwi fruit meeting method in flower period. The invention comprises the following steps: (i)cuttage time, the start time of cuttage of the male plant is 45-55 days earlier than the flower time of the female plant; (ii) preparing slotter; (iii) cutting the cuttage-ear; (iv) the management after the cuttage, removing the top when the new ear grows out; (v) making the fresh male flower meet with that of female when the kiwi fruit blooms. The invention possesses the following characteristics: the large quantity of pollen, which guarantees enough pollen supply; the change of flower period, the meeting in the flower period when the flower period varies widely can be realized by controlling the time artificially; the wide range of the use, it can be used in many basal kiwi fruit production area and can also be used to solve the problem of no-meeting in the flower period of other plants.

The invention discloses a natural hybridization method for early bud extraction of female parents of bananas. The method comprises the steps of (1) selection of a female parent material and male parent material of bananas for interplanting; (2) super-dense planting of the male parent material; (3) dwarfing of the female parent material and adjustment of the flowering period; (4) dense planting ofthe female parent material; (5) releasing of bees for pollination; (6) thinning of flowers and fruits of the male parent material; (7) bud cutting and leaf nursing of the female parents; (8) bud suction for reserving plants; (9) strengthening of field management. By means of the method, the parent materials are naturally pollinated and hybridized, and when female parent banana fruits are mature oralmost mature, hybrid seeds are collected. The method simplifies the procedures of a method for artificial cross pollination among different varieties, shortens the breeding process, reduces the labor intensity of seed production, saves labor and lowers the production cost, and the purity of produced seeds is high. By means of the natural banana hybridization method, hybrid seed production in a large area can be conducted, and the natural banana hybridization method is easy to popularize.

A method for reduplicating the pollen chromosomes of eucommia tree by inducing features that a high-temp reduplicating device of tree is used to heat the male flower buds of eucommia tree in the meiophase of pollen metrocytes at 45-47 deg.C for 2 hr to increase the 2n pollen percentage to 45-70%, especially in the diakinasis-metaphase I.

The invention discloses a rice seed production method. The method is characterized in that rice species seeds generated by the Indica-Japonica intermediate type restorer 'R900' are adopted; the Indica-Japonica intermediate type restorer 'R900' is obtained in the manner that an intermediate material large in inventory, sufficient in source, high in grain setting rate and good in grain plumpness is bred with wide-spectrum and wide-compatibility genes by utilizing the strong heterosis of Indica-Japonica subspecies, a multi-resistance pioneer germplasm with Indica-Japonica stress resistance genes polymerized is selected through high-pressure screening, and finally a high-yield, good-quality and multi-resistance-unified new rice species is selected through rice quality inspection.

The invention relates to the maize breeding field and especially relates to a pollination method for increasing maize haploid induced seed setting rate. Inducing lines or hybrid strains thereof are used as male parents, and maize material expected to obtain haploid is used as female parents; the male parents and the female parents are sowed with intervals and the male parents are sowed by stages; besides, the male parents are sowed by stages in the central region of the sowing area for artificial auxiliary segment pollination. The segment auxiliary pollination method for increasing maize haploid induced seed setting rate prevents pollination but no seed setting due to the sterility of male parents and the female parents; the adopted hybrid pollinating method is combination of natural pollination and artificial segment auxiliary pollination so that full pollination of female parents is guaranteed and the seed setting rate and induction rate are increased.

The invention discloses a breeding method of a photoperiod high-sensitive BMR sorghum sterile line, and belongs to the technical field of breeding methods of forage varieties. According to the breeding method, a BMR sorghum maintainer line serves as a female parent, photoperiod high-sensitive forage sorghum serves as a male parent, hybridization and combination of the female parent and the male parent are implemented in Sanya city, Hainan province, the photoperiod high-sensitive BMR sorghum maintainer line is bred by the aid of differences of long and short sunshine time, selection standards of the maintainer line include that non-heading or late-heading brown midrib single plants at a growth stage are selected under north spring sowing long sunshine conditions, heading brown midrib singleplants within 60 days are selected under short sunshine conditions in Sanya city, Hainan province, and continuous breeding of six generations is implemented to obtain the photoperiod high-sensitive BMR sorghum maintainer line. Backcross breeding of the maintainer line and a common sorghum sterile line is implemented, completely sterile brown midrib single plants with agronomic characters consistent with those of the photoperiod high-sensitive BMR sorghum maintainer line are selected in backcross population to obtain the photoperiod high-sensitive BMR sorghum maintainer line. According to themethod, a sorghum sterile line gene bank is enriched.

The invention discloses a celery hybrid combination method. According to the method, firstly through transformation, a plurality of celery cytoplasmic male sterile (CMS) lines with stable heredity areobtained; the CMS lines are used as female parents; an excellent selfing line is selected and determined to be used as male parents; the fine management is performed in winter; the plants are cultured into healthy and strong breeding plants; the low-temperature vernalization process is completed; in spring, according to the hybrid combination plan, several CMS female parents and corresponding male parents are subjected to simultaneous fixed planting into one seed collecting ridged field; in a period from bolting to blooming, a high-density isolation net chamber is erected above the seed collecting ridged field, so that all female and male parents in the net chamber are isolated from external pollen; then, a low-density isolation net bag is used in the net chamber for performing flower andbranch isolation on all CMS female parent plants. The artificial auxiliary pollination is performed in the blooming period; the hybrid seeds can be harvested from the CMS female parent plants when seeds become yellow. The condition that one male parent simultaneously perform hybrid combination with a plurality of CMS female parents can be realized by the method; the sufficient quantity of hybridseeds are obtained; the hybrid breeding efficiency and the plant disease and insect pest prevention and control effects are improved; the field management is convenient.

The invention discloses a selection and breeding method for a color sweet-glutinous maize inbred line, and belongs to the field of maize breeding. The method comprises the following steps: (1) hybridization is performed by using a purple-glutinous maize seed as a female parent and a white-sweet maize seed as a male parent, and therefore an F1 generation is obtained; (2) purple seeds in the F1 generation are selected and planted, backcrossing is performed by using purple-glutinous maize seeds as male parents, and therefore an F2 generation is obtained; (3) purple-glutinous seeds are selected from the F2 generation, each seed is singly planted, bagging is performed on every grown maize for self breeding, and therefore an F3 generation is obtained; (4) ears with white-sweet seeds which appear in the F3 generation are selected, all purple-glutinous seeds on the ears are selected out and the selected seeds are separately singly sown, bagging is performed on every grown maize for self breeding, and therefore an F4 generation is obtained; and (5) white-sweet seeds in the F4 generation are selected and planted, and self breeding is continuously performed for 3-4 generations according to quality, resistance and plant types, so that the color sweet-glutinous maize inbred line is bred, and the inbred line can be used to breed stock seeds through self breeding or mixed breeding. Fruits of the color sweet-glutinous maize inbred line bred by the method are sweet and glutinous, and colourful in appearances.

The invention discloses a method for inducing and identifying corn to generate haploid. The invention provides a method for inducing corn to generate haploid. The method comprises the following stepsof A) preparing a corn haploid induction line hybrid with red-root purple plant characters; B) pollinating female parent corn by taking the corn haploid inducible line hybrid obtained in the step A) as a male parent, harvesting clusters, and sowing seeds with colorless embryos to obtain seedlings; and selecting seedlings with white root systems as haploid plants. According to the induction line 'Jinyuan high hybrid induction No.1' prepared by the method, the purity of the identified corn haploid can be remarkably improved by adopting a corn haploid induction identification technology, and thepurity is as high as 100% and is far higher than that of a control group (generally about 70%). According to the method, the key technical problem that the false haploid rate is too high in the haploid identification process is solved, and therefore the haploid identification efficiency is greatly improved.

The invention relates to a method for increasing a maturing rate in sugarcane hybrid seed production, belonging to the technical field of agricultural cultivation. A generated regeneration plant after a hybrid parent is subjected to air layering in sugarcane hybrid seed production is in culture solution in a culture stage; the florescence of flower spikes of the hybrid parent is prolonged by adjusting the temperature, the nutrients and the dissolved oxygen of the culture solution; simultaneously, the pollen quantity and the developmental rate can also be increased; and finally, the maturing rate of hybrid seeds is increased. The temperature of the culture solution is 15-20 DEG C; the nutrients respectively contain 145-160mg / L of nitrogen, 38-42mg / L of phosphorus, 285-315mg / L of potassium and 3.8-4.2mg / L of boron; and the content of the dissolved oxygen is 4-8mg / L. Experiments show that the florescence of flower spikes of the sugarcane hybrid parent can be effectively prolonged by 4-7 days; simultaneously, the pollen quantity can be increased by 20-50%; the maturing rate of the hybrid seeds can be increased by 10-20%; and the method disclosed by the invention is very applied to sugarcane hybrid seed production.

The invention relates to a breeding method for a purple waxy corn inbred line. The breeding method comprises the following steps of: (1) hybridizing waxy corn varieties including the Beijing sweet and purple waxy corn No. 2 used as a female parent with the Jiangsu waxy corn No. 5 used as a male parent to obtain a hybrid cluster, and selfing a Beijing sweet and purple waxy corn No. 2 single plant to obtain a selfed cluster; (2) selecting and reserving a purple corn seed from the hybrid cluster, planting the selected corn seed to obtain a female parent, and hybridizing the female parent with a male parent which is obtained by planting a purple seed which is selected from the selfed cluster to obtain hybrid seeds; and (3) planting the hybrid seeds, bagging, selfing the plants, selecting and reserving purple seeds continuously, planting the selected purple seeds, selecting superior individual plants by combining the stress resistance and qualitative characters, and selfing 4-5 generations of the superior individual plants continuously to breed a stable purple waxy corn inbred line ZN-01. The breeding method can ensure that all the developed hybrid seeds have the characteristics of high quality, high stress resistance, wide application range and good commodity performance.

The invention provides a method for raising the induction rate of corn haploid inducing breeding.The method comprises the steps of conducting cross breeding on a corn haploid inducing line A and a corn haploid inducing line B, so that F1 serving as an inducing line cross-breeding seed C is obtained; taking C as a male parent, and taking a group D used for selecting a new material as a female parent, conducting cross breeding, and harvesting cross breeding current generation grains, so that corn haploid is obtained, wherein cross breeding of C and D comprises the following steps of watering a female parent plant with tap water with the PH value ranging from 5.5 to 8.0; conducting mist spraying treatment on filaments of the female parent plant which is watered with tap water with the same PH value with tap water with the PH value ranging from 5.5 to 8.0; after the filaments of the female parent emerge, conducting pollination cross breeding on the female parent with pollen of the male parent on the fifth day and the seventh day.By means of the method, the haploid inducing rate is effectively raised, breeding efficiency is improved, less investment is input, and the method is simple, convenient and easy to implement; meanwhile, agronomic traits of cross breeding of the inducing lines are greatly improved, and guarantee is provided for efficient and large-scale haploid induction.

The invention discloses a breeding method of a three-high and one-excellent maize inbred line (FQ920), which belongs to the field of maize breeding, and is characterized in that the maize inbred line (FQ920) is bred by taking an introduced Thailand germplasm Suwan-3 population as a basic material through an intra-population mixed powder gene recombination and multi-generation optimal selection inbreeding method, and the three-high and one-excellent maize inbred line (FQ920) is bred into the maize inbred line (FQ920). The intra-population mixed powder gene recombination and multi-generation optimal selection selfing comprises the following steps: (A) performing optimal plant selection and powder taking mixed sister cross on the basic material population, and performing optimal selection to obtain an S2 strain; (B) selecting strains with good resistance, excellent quality and high yield potential from the strains S2, and performing selfing to obtain strains S3; according to the method, the breeding procedure is relatively simple, the operability is high, the success probability is large, the investment of manpower and material resources is small, the bred corn inbred line (FQ920) has the characteristics of high yield, high resistance, high matching and high quality, and the assembled new corn variety has the advantages of high yield, high quality, multiple resistance, wide applicability and the like; the problems that high quality is not high, high yield is not high and high yield is not resistant to diseases are solved.