945results about How to "Stable traits" patented technology

The invention discloses a method for tissue-culture breeding and wild-simulated cultivation of dendrobium candidum, which comprises: inoculating dendrobium candidum tender straw slices onto a culturemedium for culture under conditions of a temperature of 22 to 24 DEG C and an illuminance of 1,000 to 1,500 Lx to first induce the growth of calles to form protocorms and then promote the multiplicative growth of the protocorms into 5 to 8 centimeter strong seedlings; and moving the seedlings out of a culture room, hardening the seedlings at a normal temperature for 3 to 5 days and transplanting the seedlings into the ground in a green house. The plane basic layer of a cultivation check field in the green house are provided with spaced breather holes; the upper layer of the cultivation check field is a cultivation medium; the seedlings are planted at a row spacing of 12*8cm and a spacing in the row of 12*8cm; in the green house, the temperature is 0 to 28 DEG C, the humidity is 60 to 70 percent and the shade density is 70 to 80 percent; and disease and pest prevention and control are carried out periodically and the dendrobium candidum can be harvested in 3 to 4 years. The method improves the civil year breeding coefficient of the dendrobium candidum by about 10 times, provides enough seedlings for large-scale cultivation, solves the problem of wild-simulated cultivation and realizes scale cultivation.

The invention discloses a nutritional health food with the function of improving gastrointestinal function and a preparation method thereof. The nutritional health food can be used for regulating the intestinal flora to improve the gastrointestinal function from the aspects of increasing the probiotics type and quantity, increasing the probiotics nutrient substance, inhibiting the growth of harmful bacteria and enhancing the immunity of human body. According to the preparation method, probiotics powder such as lactobacillus plantarum is used as the main raw material, and edible fungi extracts and modified dietary fibers are scientifically compounded; saussurea involucrata culture extracts which are capable of remarkably enhancing the immunity of human body, resisting inflammatory and easing pain, and have the functions of oxidation resistance, radiation resistance and fatigue resistance are used, so that while the intestinal probiotics flora types is increased, the fix planting capacity of the endogenous probiotics and exogenous probiotics in the intestinal tracts of human body is remarkably enhanced, and fix planting time of the endogenous probiotics and exogenous probiotics in the intestinal tracts of human body is prolonged remarkably, the growth and reproduction of the harmful bacteria, particularly the Gram negative bacteria, in the intestinal tracts are effectively inhibited, the forming of the probiotic floras in the intestinal tracts is fully regulated and the nutritional health food which is good in probiotic property, capable of remarkably enhancing the immunity of human body and has the effect of improving the gastrointestinal function is prepared.

The invention discloses a health food capable of improving intestinal floras and a preparation method of the health food. The intestinal floras are adjusted on a plurality of aspects of increasing the probiotics variety and quantity, adding probiotic nutrient substances, inhibiting the harmful bacterial growth and increasing the human immunity, so that the gastrointestinal functions are improved. With probiotic powder such as lactobacillus plantarum as a main raw material, an edible mushroom extract is scientifically compounded, and dietary fibers are modified, so that the human immunity can be significantly improved; a saussurea involucrate culture extract has the efficacies of resisting inflammation, relieving pain, and resisting oxidation, radiation and fatigue; the variety of the intestinal beneficial bacteria floras is increased; the field planting capacity of endogenous and exogenous probiotics in human intestinal tracts is significantly enhanced and the field planting time of endogenous and exogenous probiotics in human intestinal tracts is significantly prolonged; the growth and the reproduction of intestinal harmful bacteria, especially gram negative bacteria, are effectively inhibited; the components of the intestinal beneficial bacteria floras are fully adjusted; and the health food which is high in probiotic property, and is capable of significantly improving the human immunity and improving the intestinal floras is prepared.

The invention discloses a probiotic-containing health food and a preparation method thereof, wherein intestinal florae are adjusted in many aspects of increasing the types and quantity of probiotics, increasing the nutrient substances of the probiotics, inhibiting the growth of harmful fungi, enhancing human immunity and the like, so as to improve gastrointestinal functions. Probiotic powders of lactobacillus plantarum and the like are taken as main raw materials, and edible fungi extract, modified dietary fibres, a saussurea involucrata culture extract capable of remarkably enhancing the human immunity, resisting inflammation and easing pain and having anti-oxidization, anti-radiation, anti-fatigue functions and the like are scientifically compounded, and then the planting capacity and the planting time of endogenous and exogenous probiotics in human intestinal tracts are remarkably improved and prolonged, the growth and reproduction of intestinal harmful fungi, especially, Gram-negative bacterium, are effectively inhibited, and the composition of intestinal probiotic florae is adequately regulated while the types of the intestinal probiotic florae are increased; therefore, a health food with high probiotic property, capable of remarkably enhancing human immunity and containing probiotics is prepared.

The invention relates to a quick and efficient non-template agent hydro-thermal synthesizing method. The system can synthesize an alpha-molybdenum trioxide nanometer rod and a high-density echinoid molybdenum oxide based nanometer materials. Molybdenum peroxide acid prepared from molybdenum trioxide and aqueous hydrogen peroxide solution is used as a precursor, is produced into scattered alpha-molybdenum trioxide nanometer rod by hydro-thermal synthesis at a temperature of between 80 and 180 DEG C, and is produced into the peroxide modified molybdenum oxide hydrate by hydro-thermal synthesis at a temperature of between 65 and 75 DEG C. The hydrate is a multiscale structure; a nanometer thin slice, a micron prism and a nanometer rod-shaped structure unit are divergently assembled into a micron-size high-density echinoid structure. The hydrate is roasted to obtain high-density echinoid alpha-molybdenum trioxide. Modulation of the synthesizing condition can realize fine adjustment for appearance of the nanometer rod, the micron-size echinoid structure and the structure unit thereof. The method uses raw materials with low cost, has the advantages of simple technical process, controllable conditions and the like, and can promote research and application of the molybdenum oxide in the fields of sensors, field transmission, electrode materials and so on.

The invention discloses a liquid enzyme preparation and a preparation method thereof and mainly solves the technical problem that a glutamine transaminase liquid preparation is hard to preserve at the normal temperature. According to the technical scheme, the enzyme preparation is a liquid preparation of glutamine transaminase EC2.3.2.13, and the constituent content of the enzyme preparation is that enzyme activity of the glutamine transaminase liquid preparation is 10-1000 u / ml, the weight volume percentage content of a water activity conditioning agent is 30-80%, the weight volume percentage content of a redox potential conditioning agent is 0.0075-1%, the weight volume percentage content of a food preservative is 0-0.1%, and a pH conditioning agent is supplemented to 100% in volume. The preparation method of the liquid enzyme preparation comprises the steps of enzyme liquid purification, mixing, sterilization and bottling to obtain a finished product. By means of the liquid enzyme preparation and the preparation method thereof, the enzyme activity of the enzyme preparation preserved for six months at the normal temperature can be maintained at 80% or above, the character is stable, no microbial spoilage is caused, and the using effect is good.

The invention relates to a low-lactose liquid milk product containing fish oil and a production method thereof. The low-lactose liquid milk product containing fish oil is prepared from the following materials in parts by weight: 700.0-999.74 parts of cow milk, 0.1-12 parts of fish oil, 0-30 parts of oligosaccharide, 0-100 parts of dietary fiber, 0-3 parts of nutrients, 0.05-5 parts of lactase, 0-10 parts of an emulsifier, 0-5 parts of a thickener, 0-1 parts of an acidity regulator, 0.01-1 part of an antioxidant, 0-5 parts of edible essence, and the balance of pure water. According to the fish oil-containing low-lactose liquid milk product and the production method thereof, the stability problem of the fish oil in the liquid milk product is successfully solved by reasonably screening the proportion of various components, and the product taste and the content of the nutrients are stable within a shelf life. The high nutritional value of the product can satisfy the nutritional needs of the children, the youngsters, the office workers and the middle aged and elderly people, so the low-lactose liquid milk product containing fish oil has a strong market prospect of sale.

The invention belongs to a medlar general flavone extracting and purifying technology and a medlar general flavone detecting method as well as applications of medlar general flavone. At present, the method for preparing medlar general flavones comprises a reflux extracting method, an ultrasonic wave extracting method and a microwave extracting method. According to the extracting and preparing method of flavones in medlar leaves disclosed in 201010542616.3, a colloid breaking-in technique, a high-pressure homogenizing technique and a membrane separation and combination application technique are adopted, and the preparation is complex and long time is consumed. The invention aims to research the medlar general flavone extracting and purifying technology, the medlar general flavone detecting method as well as applications of the medlar general flavone. The technical scheme provided by the invention comprises the following steps of conducting an extracting technology: drying and crushing medlar, adding alcohol, extracting and combining filter liquor, concentrating, and extracting, thereby obtaining a medlar flavone rough extract; and conducting an extracting and purifying technology: purifying the rough extract by adopting porous resin D101, and further purifying the flavones by adopting polyamide. The medlar general flavone extracting and purifying technology, the medlar general flavone detecting method as well as applications of the medlar general flavone provided by the invention have the effects that general flavones are extracted as well as polysaccharide is obtained, effective ingredients of medlar are extracted fully, and foundation for large-scale industrial production is laid.

The invention belongs to the field of fertilizer industry and discloses synergistic calcium superphosphate and a preparation method and application thereof. The preparation method comprises the steps of preparing organic acid, amino acid or other small-molecular organic matters containing a large number of low molecular weights by utilizing acid hydrolysis of agricultural wastes and secondary hydrolysis of wastes from a food processing plant, then taking the substance as a synergist I, then adding calcium superphosphate into the synergist I, and uniformly drying and crushing to obtain powdery sustained-release synergistic calcium superphosphate; or uniformly mixing the synergist I, a suspending agent and an emulsification coating agent to prepare a synergist II, then adding calcium superphosphate into the synergist II, and performing high-speed shearing and uniform mixing by a colloid mill to obtain liquid sustained-release synergistic calcium superphosphate. The synergistic calcium superphosphate obviously reduces the opportunity of adsorbing phosphorus by soil colloid or fixing phosphorus by iron ions and the like, increases the effectiveness of phosphorus and improves the utilization rate of fertilizer. The method has the advantages of simplicity, convenience, easiness in operation, capability of saving energy and raw materials and effect of reducing production cost. The synergistic calcium superphosphate disclosed by the invention can be applied to the field of agricultural production of vegetables, fruit trees and the like.

The invention belongs to a preparation method of high economic fish micro-spheres opening bait, specifically it uses sodium alginate and starch as micro-spheres binding material and embeds bait nutritional component through air and mist means. It protects vitamins, proteins, inorganic salts and other material components away the influence and interreaction of heat, moisture, oxygen, ultraviolet rays and metallic ion during the process of processing, storing, transporting and feeding. It controls the particle diameter of opening bait using micro-spheres particle diameter to fitting the first ingress of fish fry. It could reduce loss rate of bait nutrient components in water, maintain water clean, improve survival rate of economic fish seedlings and reduce the production costs of artificial breading.

The invention discloses a processing method for a black tea fungus solid beverage. The processing method sequentially comprises the following steps: (1) preparing tea soup; (2) inoculating; (3) fermenting; (4) centrifuging; (5) sterilizing; (6) spraying and drying, namely adding a maltodextrin drying aid into concentrated liquid and uniformly mixing; and then drying by using a spraying dryer under the condition that an air inlet temperature is controlled at 150-180 DEG C, an air outlet temperature is controlled at 70-90 DEG C, and the water content is 5-7% after spraying and drying, thus preparing black tea fungus dried powder; and (7) blending and putting into bags, namely mixing and blending following materials into mixed powder I in percentage by weight: 1%-3% of instant black tea powder, 2%-3% of citric acid, 0.15%-0.45% of sodium citrate, 90%-96% of white granulated sugar powder; uniformly mixing the mixed powder I and the black tea fungus dried powder according to the ratio of (15%-50%) to (85%-50%) to obtain mixed powder II; and putting the powder into the bags. The sterilization treatment guarantees the stability of product properties and the product obtained by concentration and drying procedures is convenient to store and transport.

The invention discloses a Chinese medicine composition for prevention and control of porcine circovirus disease and a preparation method thereof, and belongs to the field of traditional Chinese medicine for livestock. The Chinese medicine composition is composed of twenty traditional Chinese medicines comprising honeysuckle flower, bupleurum, Herba Houttuyniae, Radix Isatidis, Radix Astragali, licorice, fructus forsythiae, wolfberry fruit, dried tangerine peel, baikal skullcap root, epimedium, bighead atractylodes rhizome, cucumber, rhizoma atractylodis, Folium Isatidis, Polyporus umbellatus, angelica, Codonopsis pilosula, Poria and Rhizoma Coptidis. An injection is prepared by steps of crushing, decoction, enzymolysis, flocculation precipitation, concentration, filtration, sealing and sterilization. The pure natural herbs adopted by the invention are safe, reliable and without toxic or side effects; compatibility of the medicines is in line with the principle of assistant and guide; and the composition is safe, and has no toxic or side effects, and the efficacies of clearing heat, removing toxicity, resisting bacteria, relieving inflammation, and enhancing immunity of the body. The prepared injection has high bioavailability and stable traits, can effectively enhance immunity of the body, and has good treatment effect on porcine circovirus disease.

A process for preparing arachidonic acid oil by microbial fermenting includes culturing and activating Mortierella alpina (M0223), culturing seed, inoculating the seed in shake flask or fermentor, fermenting, collecting wet thallus, baking and extracting oil. Its advantages are high purity and safety.

The invention discloses a method for breeding a new golden-shell crassostrea gigas strain. The method is characterized by including the operating steps of (1) breeding the first generation: selecting wild parent crassostrea gigas with golden left shells, and creating a plurality of families without genetic relationships; (2) breeding the second generation: selecting the families with the obvious golden-shell characters from the created first-generation families, and screening out the crassostrea gigas with the golden left shells and the golden right shells to carry out inner-family inbreeding; (3) breeding the third generation: carrying out inter-family hybridization on the families, with the left shells and the right shells being in the golden characters, obtained from the created second-generation families; (4) breeding the fourth generation: carrying out stability verification on the families with the left shells and the right shells being in the golden characters. According to the method, the breeding process of the new crassostrea gigas shell-color strain is accelerated, and new products of bred shellfishes are increased; the product additional value is increased through the color characters, the breeding yield is improved through the growth characters, incomes of breeding enterprises and farmers are increased, and the market prospects are broad.

The invention discloses a bag expansion diameter-variable steel reinforcement cage. The bag expansion diameter-variable steel reinforcement cage comprises an axial rod, a circular ring or an annular plate, several longitudinal ribs, several rib strips and a ring-shaped fixator, the circular ring or the annular plate is perpendicular to the axial rod, one end of each longitudinal rib is uniformly fixed to the circular ring or the annular plate, the other end or the middle of each longitudinal rib is connected to one end of a corresponding rib strip, several longitudinal ribs surround the axialrod, the other end of each rib strip is connected to the ring-shaped fixator, and the ring-shaped fixator is fixed to or slides on the axial rod or a pile foundation rod; an outer bag covers the bottoms of the several longitudinal ribs, at least the bottom of the bag is sealed, and the bag with an expanded pile bottom end covers several longitudinal ribs; at least one slurry injecting opening anda slurry outlet with a one-way valve are formed in the bag.

The invention discloses an automatic meat paste hammering machine, and relates to a machine automatically hammering massive raw meat into meat paste. The machine overcomes the defects that the fitness of square iron bars and an anvil face cannot be mastered well, the fineness, the elasticity and the like of the meat paste are not stable, the meat paste is easily deteriorated after hammering heating, and the physical energy is consumed when the massive raw meat is manually hammered into the meat paste with the two square iron bars in the current local characteristic technology of manual meat ball hammering. The machine is characterized in that a hammering area is formed by hammering a rotating anvil face arranged below in a fixed direction with a rectangular hammering bar in an extending hammering device of an air hammer; a used baffle device turns over the massive raw meat to the hammering area during rotation of the anvil face and avoids overflow; a coolant circulation cooling loop in the rectangular hammering bar reduces a heating state of the massive raw meat or the meat paste on the anvil face in a hammering process and improves the quality of a finished product until the massive raw meat is completely and uniformly hammered into the finished meat paste; and the characteristics of fine and elastic hammered meat paste and the like are shown better.

The invention discloses a feed sweetener, comprising the following components by parts: 70-95 parts of saccharin sodium, 2-5 parts of synergist, 0.2-1.0 part of flavour enhancer and 2-25 parts of glucose, wherein, the synergist is ASP sweet, AK sugar, thaumatin, stevioside and / or maltol; the flavour enhancer is neotame. The feed sweetener is produced by the compound fusion-spray drying method. The feed sweetener has high liquidity, good mouthfeel of sucrose, lasting and stable sweet taste effect and sweet scent flavour. Being added into the feed, the feed sweetener can improve the feed palatability and is suitable for various growth stages of animals.

Disclosed is the high quality germchit tissure culturing and rapid breeding method of Dendrobium sp., wherein a large amount of test tube seedlings with stabilized performance are obtained through fine breed tissue culture by adopting the path of clumping buds. The produced Dendrobium sp. has the advantages of high genetic stability, quick emergence of seedlings, strong seedlings, good seedling quality, strong resistance, and fine growth status.

The invention discloses a cordyceps militaris strain for producing cordycepin with high yield and belongs to the technical field of bioengineering. The cordyceps militaris strain is named as cordyceps militaris JN168 by classification and is preserved with CCTCC No: M2011333 inChinaCenter for Type Culture Collection. The cordyceps militaris strain which is stable in character is obtained by isolation and screening of wild cordyceps militaris sporocarps collected from Antu, Jilin Province. The cordyceps militaris strain is capable of producing the cordycepin by liquid-state fermentation at the fermentation temperature of 30 DEG C, and the final concentration of cordycepin can be 6g / L after fermentation for 5 days.

The invention discloses a butterfly orchid breeding method. The butterfly orchid breeding method comprises hybridization pollination, seed culture, young seedling plantation, middle seedling plantation and large seedling plantation. In the fourth month after the hybridization pollination, pods can be harvested. In the fifth-sixth month after aseptic sowing, seedlings can be taken out of a culture bottle and can be planted. The seedlings are planted in a greenhouse. Through control of environment conditions, the seedlings can grow fast. The time from seedling planting to plant maturation is in a range of about 10-12 months. The time from flower forcing to novel breed selection is controlled in 70-100 days. The time of the whole breeding process is controlled in about 22-25 months. The butterfly orchid breeding method can control all processes of the whole breeding process and realizes fast selection of a novel breed.

The invention provides a silicone oil-containing hair conditioner good in stability, comprising silicone oil, an emulsifier, a conditioning agent, a thickener, additives and deionized water. The emulsifier is a polymer having a molecular weight of 1000 to 5000 thousands and obtained by inverse emulsion polymerization of methacryloyloxyethyl trimethyl ammonium chloride and docosyl acrylate; the conditioning agent comprises polyquaternium and a small-molecular cationic surfactant; the thickener is a fatty alcohol; the additives include a preservative and a pH regulator; the components in percent weight in the conditioner are: the silicone oil 0.1-30.0%, the high-molecular emulsifier 0.5-4.0%, the polyquaternium 0.5-10%, the small-molecular cationic surfactant 0.1-8.0%, the fatty alcohol 0.5-10.0%, the preservative 0.1-0.3%, and the pH regulator 0.1-0.25%. The silicone oil-containing hair conditioner has a reasonable formula, a paste of the conditioner never thickens or bleeds during low-temperature storage, the conditioner can neutralize negative charge of shampoo remaining in hair, can smoothen the tangly hair and enable the hair to be easily combed, can impart gloss and smoothness to the air. The invention also provides a preparation method of the silicone oil-containing hair conditioner.

The invention discloses guanidine hydrochloride sustained release preparation and a preparation method thereof. The guanidine hydrochloride sustained release preparation is mainly prepared by guanidine hydrochloride, a filling agent, sustained release materials and potential of hydrogen (pH) sensitive materials. The preparation method comprises the steps of firstly preparing all raw materials according to proportion, evenly mixing the guanidine hydrochloride, the filling agent, the sustained release materials and the pH sensitive materials at high speed, granulating and drying the evenly mixed powder, finally adding a flow agent, a lubrication agent and an adhesion agent, tableting according to a general method, and achieving guanidine hydrochloride sustained release tablets. The guanidine hydrochloride sustained release preparation is small in side effect, obviously reduces difference of preparation of different batches, improves stability of samples, is convenient long term curing of patients and improves compliance of medicine. A patient can take the guanidine hydrochloride sustained release tablets once a day, so that effective drug concentration in bodies can be guaranteed for 24 hours, and nervous centralis side reactions caused by the fact that a blood concentration peak value is high due to general preparation is reduced.

The invention discloses a method for using supercritical carbon dioxide for extraction of chrysanthemum essential oil, dry and crushed chrysanthemum is used as a raw material, the supercritical carbon dioxide uses anhydrous ethanol as an entrainer to extract a chamomile essential oil raw thick liquid, then the raw thick liquid is frozen, filtered and concentrated to obtain the high purity chamomile essential oil. The high purity chamomile essential oil product has high extraction yield, high purity, no toxicity, no residue, pure color, stable characters, pure naturalness and other characteristics, and is simple in process and low in energy consumption.

The invention relates to a medlar composite beverage which comprises 55% of medlar leach liquor, 8% of red date leach liquor and 12% of apple leach liquor. According to the invention, medlar with multiple health protection functions, red dates with rich vitamins and apples with special fruit flavor are complemented in nutrition and flavor so as to prepare the composite beverage which has unique flavor, and is sour and tasty, rich in nutrition and stable in property. According to the invention, boiling water is adopted for leaching, and effective ingredients in the raw materials can be utilized fully and effectively, so that on the one hand, the produced medlar composite beverage has functions of medical health protection and immune improvement of the medlar, on the other hand, the mouthfeel and the flavor of the beverage are enriched through the red dates and the apples, and the efficacies and nutritional values of the medlar beverage are improved, Meanwhile, the successful development of the medlar composite beverage provided by the invention not only provides a novel development direction for the research and development of the medlar beverage, but also opens a novel way for the development and utilization of medlar resources.

The invention relates to a nutrient freeze-drying production method, which comprises: making the nutrient into emulsion in a emulsifying machine; putting the emulsion into a vacuum freeze-drying chamber for vacuumizing and quick-freezing; sublimating by heating to obtain a dry anhydrous solid matter; and grinding the freeze-dried solid matter into powder in a grinding machine. By making the nutrient into powder by vacuum freeze-drying technology, the invention avoids the generation of peroxides and trans-fatty acid which is harmful to human body cuased by the oxidation of grease and fat-soluble nutrients, consequently, protects the active components of nutritious oil and fat-soluble nutrients. The powder made by the method achieves good stability and a long shelf life.

The invention relates to an oyster and kudzu root natural anti-alcohol agent and a preparation method thereof. The method comprises the following steps: (1) adding water in oyster, crushing and homogenizing, adjusting pH value of slurry, carrying out enzymolysis, refrigerating and centrifuging hydrolysate after the completion of enzymolysis to obtain supernatant, and spray-drying or freeze-drying in vacuum to obtain oyster extract powder; (2) grinding kudzu root, adding water, leaching in constant-temperature water bath, carrying out rough filtration to obtain leaching liquor, leaching residue again, filtering to obtain filtrate, merging the filtrate obtained for two times, adding amylase in the filtrate, stirring, placing in the constant-temperature water bath, carrying out enzyme inactivation using boiling water, carrying out multiple centrifugation to obtain light yellow kudzu root extracting solution, and spray-drying or freeze-drying to obtain kudzu root extract powder; and (3) mixing the oyster extract powder with the kudzu root extract powder in propart to obtain the oyster and kudzu root natural anti-alcohol agent. The raw materials of the method are very wide in sources and rich in nutrition, can protect liver, calm the nerves and clear away heat and toxic materials, are easy to be absorbed by the body without toxic or side effects and with high safety, and can be prepared into products in multiple forms.

The invention provides a heatproof lyoprotectant for a live vaccine against pseudorabies. The heatproof lyoprotectant comprises, by weight, 3 to 10 parts of gelatin, 1 to 5 parts of trehalose, 5 to 15 parts of sucrose, 0.1 to 2 parts bovine serum albumin, 1 to 8 parts of tryptone, 2 to 10 parts of enzyme-hydrolyzed casein, 1 to 5 parts of thiourea, 0.8 to 2 parts of L-monosodium glutamate, 0.1 to 3 parts of arginine, 0.5 to 5 parts of polyvinylpyrrolidone (PVP-K30) and 0.1 to 2 parts of mannitol. The invention also discloses a preparation method of the heatproof lyoprotectant and a lyophilized vaccine prepared from the heatproof lyoprotectant. When the heatproof lyoprotectant is used for protecting the vaccine and a specific lyophilization process is employed, lyophilization loss of viruses can be effectively reduced, the temperature tolerance of the viruses can be improved, and the vaccine can still maintain good physical properties and titer after long-term storage; i.e., the vaccine has stable characters and has the characteristics of heat resistance and long storage time.

The invention relates to an application of an esophageal squamous cell carcinoma primary tumor strain CH-H-1 in a mammal for producing esophageal squamous cell carcinoma cells. The primary tumor strain CH-H-1 disclosed by the invention can realize multiple passages through NOD / SCID mice, has significance for solving the bottleneck that the current treatment of the esophageal squamous cell carcinoma can not avoid distant metastasis, and is an ideal object for fundamental research on the esophageal squamous cell carcinoma and clinical early application.

The invention provides a human pancreatic cancer cell line and its preparation method and application. The human pancreatic cancer cell line is preserved in the China Center for Type Culture Collection and the preservation number is CCTCC C201007. The human pancreatic cancer cell line can be utilized for producing human pancreas cancer in mammals, preparing human pancreas cancer models and screening candidate drugs for treating human pancreas cancer. The human pancreatic cancer cell line has stable characters and is subcultured multiple times. Characters are stably maintained in 20th to 50th generation obtained by subculture in vitro. The human pancreatic cancer cell line has clinical biological characters of human pancreas cancer and provides an experimental material for pancreas cancer research, wherein biological characteristics of the experimental material are close to clinical tumor biological characteristics. Compared with a subcultured parent tumor in a nude mouse, the human pancreatic cancer cell line can be utilized for analysis of correlation of tolerance and susceptibility to drugs in vivo or vitro thus can establish two associated anti-pancreatic cancer drug screening platforms in vitro and in vivo.

The invention provides a green tangerine orange peel reference extract which is prepared by the following steps: carrying out multiple extraction on different sources or batches of green tangerine orange peel drug powders to obtain different sources or batches of green tangerine orange peel extracts, and blending the different sources or batches of green tangerine orange peel extracts to obtain the green tangerine orange peel reference extract. The green tangerine orange peel reference extract is blended from different sources or batches of extracts, thereby ensuring the consistency of different batches of green tangerine orange peel reference extracts. By selecting the representative drug as the raw material, the obtained product is representative, and can reflect the quality of the detected drug more visually when being used as the reference extract. The green tangerine orange peel reference extract has stable and uniform properties, and is convenient to use. The green tangerine orange peel reference extract can be used as the reference in the quality control of the green tangerine orange peel and drug preparations containing green tangerine orange peel, can be used for qualitative identification on the drug or traditional Chinese medicine preparation, and can also be used for semiquantitative and even quantitative analysis.