12results about How to "Stable traits" patented technology

This invention discloses a murine undifferentiated thyroid cancer cell line and its applications. The invention provides a murine undifferentiated thyroid cancer cell line, MATC (Mus musculus), deposited on October 10, 2024, at the China Center for Type Culture Collection (CCTCC), with accession number CCTCC NO: C2024159. The MATC murine undifferentiated thyroid cancer cell line of this invention exhibits good adherence and stable cell characteristics, allowing for stable multiple passages. STR identification confirms it as a novel single cell line, enriching the library of undifferentiated thyroid cancer cell lines. It provides new experimental materials with biological characteristics closely resembling clinical tumors for research on undifferentiated thyroid cancer, serving as an effective target cell line for target research. It possesses very strong in vitro tumorigenic ability and can successfully prepare animal models of undifferentiated thyroid cancer for basic research and drug screening.

This invention discloses a method and microbial preparation for controlling mercury accumulation in rice grains using Metarhizium anisopliae colonization, belonging to the field of agricultural environment and microbiology technology. The method involves inoculating rice plants with Metarhizium anisopliae spores, followed by planting the rice in mercury-contaminated paddy fields; water management is implemented throughout the rice's growth cycle until harvest. This invention establishes a complete microbial remediation system for mercury contamination in paddy fields, providing not only a method for the colonization and application of Metarhizium anisopliae in mercury-contaminated environments, but also ensuring safe rice production, offering a feasible approach to safeguarding food security and human health.

The invention discloses a method for cultivating high-starch potatoes through grafting, and relates to the technical field of potato cultivation. The method comprises the following specific steps: (1) cultivating a high-starch potato stock and a common potato scion; (2) grafting the rootstock and the scion; (3) grafted plant treatment; (4) domestication treatment: carrying out stress treatment on the grafted seedlings for 10-15 days, then transferring the grafted seedlings outdoors for culture, and harvesting tubers; and (5) screening of high-starch germplasm: selecting tubers with high starch content for planting, repeatedly domesticating for 2-3 generations, and harvesting tubers to obtain the high-starch potato germplasm. According to the method, the high-starch potato seedlings and the common potato seedlings are grafted, aeroponic culture management is performed after grafting, domestication treatment is performed, the starch content of the grafted potatoes can be increased, and after multi-generation domestication, the high-starch potato germplasm is obtained through breeding. According to the method, the variety breeding process is shortened, and the problem that the high-starch potato germplasm is not created by adopting a grafting method at present is solved.

This invention discloses a plant-derived herbicide and its preparation method, specifically relating to the field of biological weed control. The whole plant of *Coriandrum sativum* is dried, pulverized, and sieved. After ultrasonic extraction with an organic solvent, the extract is concentrated to obtain a paste-like extract. This plant-derived herbicide contains the following raw materials in volume fractions: 10 parts *Coriandrum sativum* extract, 2 parts emulsifier, and 88 parts water. The technical solution of this invention solves the problem of environmental pollution and pesticide residues exacerbated by existing chemical herbicides. Furthermore, the plant-derived herbicide of this invention, after being prepared into a stable formulation, can effectively control annual weeds, achieving a control effect of over 75% on weeds in farmland. The application of this plant-derived herbicide can significantly reduce the amount of chemical herbicides used, thus reducing environmental pollution.

The application belongs to the technical field of biological probes, and relates to a PD-L1 guided specific targeted CTC detection combined with tumor boundary distinguishing biological probe and a preparation method thereof. The PD-L1 guided specific targeted CTC detection combined with tumor boundary distinguishing biological probe comprises a core, a covalent organic framework and a PD-L1 antibody, the core comprises a superparamagnetic nanoparticle, the covalent organic framework is coated on the surface of the core, and the PD-L1 antibody is combined on the surface of the covalent organic framework. The PD-L1 guided specific targeted CTC detection combined with tumor boundary distinguishing biological probe prepared by the application can be transported into the body across the membrane, smoothly enter a PD-L1 positive malignant tumor lesion, and perform Raman imaging to microscopically distinguish the tumor boundary.

This invention, entitled "A Method for Preparing an Antiepileptic Carbon Dot Suspension," belongs to the field of pharmaceutical technology. It relates to a method for preparing a nanoparticle suspension and provides a carbon dot suspension for treating epilepsy. The raw materials and excipients of the suspension include: carbon dots from human hair, stabilizers, flavoring agents, and preservatives. The suspension of this invention exhibits stable properties, simple excipient composition and preparation process, and excellent taste. The most preferred formulation is: 5g of carbon dots from human hair, 0.125% CMC-Na, 0.05% ethylparaben, and 2.5% sucrose.

The present application relates to a kind of yunnan ganoderma strain HL45 and its cultivation method and application, belong to the field of microbial technology.The strain has been preserved in Guangdong Provincial Microbial Culture Collection Center on September 11, 2024, and the preservation number is GDMCC No:65119.The present application yunnan ganoderma strain HL45 fruiting body presents single or multi-branch, cap purple brown, flesh white, and stable in character;The content of triterpenoid ganoderol F and glucosan polysaccharide is very high, which can be used for preparing antitumor drugs.In addition, the fruiting body of the strain has strong mushroom aroma, and the quality of the whole mushroom is good.The average growth cycle of cultivation bag is 26d, the mycelial growth rate is 0.78cm / d, the average yield is 16.62g / bag, the highest biological efficiency reaches 10.26%, the cultivation period is about 4-5 months, and it is suitable for cultivation in high-altitude areas above 2000m, with high ornamental and application value.

The invention relates to an application of an SlCLE3 gene and polypeptide thereof in improvement of salt tolerance of tomatoes. Specifically, the nucleotide sequence of the SlCLE3 gene is as shown in SEQ ID NO: 1. According to the invention, an overexpression vector of the SlCLE3 gene is constructed, and an agrobacterium tumefaciens-mediated genetic transformation method is utilized to obtain a transgenic tomato homozygous strain overexpressed by the SlCLE3. Under the condition of salt stress, the transgenic line shows significantly enhanced salt tolerance, specifically, active oxygen accumulation is reduced, cell damage is reduced, photosynthetic indexes are improved, and relative conductivity is reduced. In addition, the damage of salt stress to tomato plants can be effectively relieved by exogenously applying the pCLE3 polypeptide. The invention provides a new gene resource and a breeding method for tomato salt-tolerant breeding.

The present application relates to a kind of renal cancer therapeutic DC vaccine based on the immune peptide activation of HERV-E protein source and its preparation method, and the preparation method includes the following steps: obtaining immature DC cell;Synthesis HERV-E protein source immune peptide;Immune peptide and immature DC are co-cultured in DC activation culture medium for 1 day to DC maturation.The renal cancer therapeutic DC vaccine obtained by the way of immature DC loaded with HERV-E immune peptide is more stable in character, and has strong anti-tumor capacity, can effectively inhibit the progress of renal cancer.

The application discloses CsPHO1;H3 The application relates to the fields of plant genetic engineering and vegetable breeding technology, and discloses application of a gene in improving photosynthetic efficiency and energy metabolism of cucumber seedlings. CsPHO1;H3 The application proves for the first time that overexpression of the gene can positively improve photosystem II efficiency and net photosynthetic rate of cucumber, and can improve TP, ATP and ATP / ADP, and optimize cell energy state, and the photosynthetic improvement is not dependent on stomatal regulation, and does not increase transpiration water consumption, and the plant is more stable and stronger in resistance. The application has clear gene function, and the obtained cucumber seedlings are stable in traits, and can be directly used for cucumber transgenic breeding, gene editing breeding and molecular marker assisted breeding.

The invention relates to the technical field of compound microbial agents, in particular to a compound microbial agent with a broad-spectrum nitrogen fixation function, which comprises at least two anaerobic clostridium bacteria and photosynthetic bacteria, the anaerobic clostridium bacteria comprise Zn2T and M3 / 9T, the 16S rDNA sequence of the Zn2T is shown as SEQ ID NO.1, and the 16S rDNA sequence of the M3 / 9T is shown as SEQ ID NO.2; the photosynthetic bacteria are rhodopseudomonas palustris, and the 16S rDNA (ribosomal deoxyribonucleic acid) sequence of the photosynthetic bacteria is shown as SEQ ID NO.3. The photosynthetic compound microbial agent provided by the invention has the effect of remarkably increasing the crop yield.

This invention relates to the fields of genetic engineering and plant biotechnology, specifically to a method for single expression cassette knockout and overexpression of multiple genes. This method constructs a single expression cassette comprising a constitutive promoter, a Cas9 gene, a linker CAATCAAAC, multiple overexpressed genes, multiple sgRNA expression cassettes, and a terminator. Plants are transformed using Agrobacterium-mediated transformation, achieving simultaneous knockout of multiple endogenous gene sites and overexpression of multiple exogenous genes in a single genetic transformation. The linker connects the Cas9 gene to the overexpressed genes and among the overexpressed genes themselves. This invention offers advantages such as ease of operation, high efficiency, short cycle time, and the ability to simultaneously regulate complex metabolic pathways, making it of significant application value in crop genetic engineering and metabolic breeding.