39results about How to "Stable traits" patented technology

The invention discloses a bacillus subtilis strain and application thereof, the bacillus subtilis strain is bacillus subtilis X-NY1 and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20242038. Bacillus subtilis X-NY1, bacillus velezensis and bacillus amyloliquefaciens are compounded into a microbial agent, a film-forming agent, a dispersing agent, a coloring agent, a stabilizing agent, a preservative, a conditioning agent and a nutritional agent are further added to prepare the compound microbial seed coating agent, the effective viable count of the microbial agent is 108-1011, and the quality guarantee period is longer than 6 months. The compound microbial seed coating agent can improve the plant height, root length, fresh weight and the like in the growth process of pennisetum alopecuroides, can effectively improve the stress resistance of the pennisetum alopecuroides under the salinization condition, and is small in addition amount, low in cost, safe, reliable, obvious in growth promoting effect and suitable for application and popularization.

The invention discloses a breast adenoma B3 type source cell strain as well as construction and application thereof, the breast adenoma B3 type source cell strain is named as human breast adenoma cell Hthy2301 Homo sapiens and is preserved in China Center for Type Culture Collection on March 14, 2024, and the preservation number is CCTCC NO: C202478. The human thymoma cell line is stable in character, can be passaged in vitro for a long time, and has high proliferation activity. The human thymoma cell line disclosed by the invention can be used as an effective cell model, and a new experimental model can be provided for deeply researching the occurrence, development and transfer mechanisms of thymoma, the drug resistance mechanism, drug screening, drug effect evaluation and the like.

The invention belongs to the technical field of microbial medicine, and provides a primary multidrug resistance human far-end bile duct cancer cell line CBC3T-6 Homo sapiens and application thereof.The human far-end bile duct cancer cell line CBC3T-6 Homo sapiens is separated from differentiated adenocarcinoma in a far-end bile duct, the human far-end bile duct cancer cell line CBC3T-6 Homo sapiens is already subcultured to 50 generations at present, the long-term in-vitro culture character is stable, and on the premise that the biological characteristics of bile duct cancer are reserved, the human far-end bile duct cancer cell line CBC3T-6 Homo sapiens can be used for preparing the primary multidrug resistance human far-end bile duct cancer cell line CBC3T-6 Homo sapiens. The compound has the characteristics of short multiplication time, high mouse subcutaneous tumor formation rate and the like, and has different degrees of drug resistance to gemcitabine, 5-fluorouracil, oxaliplatin and cis-platinum in first-line anti-cancer drugs for bile duct cancer. The method not only enriches an extrahepatic bile duct cancer cell bank, provides a new cell model for basic research and clinical drug research and development of bile duct cancer, but also provides powerful scientific research data for research developed based on the genetic background of Chinese population. Therefore, the primary multidrug resistant cell line has higher scientific research and clinical application values.

The invention discloses 3D printing biological material ink based on a drug sustained-release polycitrate material and a preparation method and a printing method of the 3D printing biological material ink, and relates to the technical field of 3D printing biologication.The 3D printing biological material ink is prepared from a medical polycitrate polymer, nano-hydroxyapatite and an active drug ingredient; the addition amount of the hydroxyapatite is 5-65% of the total mass of the obtained biological material ink; the active pharmaceutical ingredients are gastrodin, notoginsenoside, recombinant wnt3a protein and LL-37 peptide; when the active medicines are gastrodin and notoginsenoside, the addition amount of the active medicines is 4-12% of the total mass of the obtained biological material ink; when the used active drugs are recombinant wnt3a protein and LL-37 peptide, the addition amount of the active drugs is 0.001-0.002% of the total mass of the obtained biological material ink; and the particle size of the nano hydroxyapatite is 60-80 nm. According to the method, 3D printing process parameters are selected, post-treatment is adopted to adjust a cross-linked network structure of a poly-citrate polymer, biological material scaffolds with different mechanical properties and degradation properties are obtained, and stable and long-acting release of carried drugs is ensured.

The invention discloses an estradiol valerate sugar-coated tablet and a preparation method thereof. The estradiol valerate sugar-coated tablet sequentially comprises a tablet core, a powder coating layer, a first sugar coating layer, a coloring layer and a second sugar coating layer from inside to outside, the tablet core contains estradiol valerate; the powder coating layer is prepared from the following components in parts by weight: 1.7 to 3.7 parts of polyethylene glycol 6000, 25 to 35 parts of cane sugar, 0.1 to 0.4 part of povidone K90, 10 to 18 parts of calcium carbonate and 4 to 10 parts of talcum powder. According to the invention, crystallization of cane sugar is effectively inhibited, the surface of a powder coating layer is finer and smoother, the undesirable phenomenon of tablet edge cracking is avoided, and the quality defects of uneven color, color fading and the like caused by moisture absorption are also avoided.

The present invention relates to a positive electrode electrolyte having a redox couple of Fe(CN) 6 3‑ / Fe(CN) 6 4‑ The invention discloses a method for recovering the positive electrode electrolyte of an alkaline iron-based liquid flow battery, which specifically comprises: taking the positive electrode electrolyte after the battery / stack is operated as the recovery object, adding a green reducing agent therein, and reducing the Fe(CN) accumulated in the positive electrode electrolyte to 6 3‑ Reduction, so that the Fe(CN) accumulated in the positive electrode electrolyte 6 3‑ Reduction to Fe(CN) 6 4‑ The present invention solves the problem of Fe(CN) accumulated in the positive electrode electrolyte during the operation of the battery / electrical stack by adding a green reducing agent to the positive electrode electrolyte after the battery / electrical stack is in operation. 6 3‑ The problem of capacity and efficiency attenuation caused by this can be solved, and the battery electrolyte can be reused.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to fluocinolone hydroquinone tretinoin acid emulsifiable paste and a preparation method thereof.The fluocinolone hydroquinone tretinoin acid emulsifiable paste is prepared from fluocinolone, hydroquinone, tretinoin, a matrix, a preservative, a humectant, an antioxidant, a thickener and an emulsifier; wherein the humectant is a mixed humectant of glycerol and methyl glucitol polyether-10 in a mass ratio of (1: 1.5)-(1: 3), and the quality of the cream product is favorably improved. In addition, by limiting the feeding sequence and the feeding temperature of the raw materials and the auxiliary materials in the preparation process, the fluocinolone hydroquinone tretinoin acid cream which is good in product quality and stable in release can be obtained.

This invention discloses a murine undifferentiated thyroid cancer cell line and its applications. The invention provides a murine undifferentiated thyroid cancer cell line, MATC (Mus musculus), deposited on October 10, 2024, at the China Center for Type Culture Collection (CCTCC), with accession number CCTCC NO: C2024159. The MATC murine undifferentiated thyroid cancer cell line of this invention exhibits good adherence and stable cell characteristics, allowing for stable multiple passages. STR identification confirms it as a novel single cell line, enriching the library of undifferentiated thyroid cancer cell lines. It provides new experimental materials with biological characteristics closely resembling clinical tumors for research on undifferentiated thyroid cancer, serving as an effective target cell line for target research. It possesses very strong in vitro tumorigenic ability and can successfully prepare animal models of undifferentiated thyroid cancer for basic research and drug screening.

This invention discloses a method and microbial preparation for controlling mercury accumulation in rice grains using Metarhizium anisopliae colonization, belonging to the field of agricultural environment and microbiology technology. The method involves inoculating rice plants with Metarhizium anisopliae spores, followed by planting the rice in mercury-contaminated paddy fields; water management is implemented throughout the rice's growth cycle until harvest. This invention establishes a complete microbial remediation system for mercury contamination in paddy fields, providing not only a method for the colonization and application of Metarhizium anisopliae in mercury-contaminated environments, but also ensuring safe rice production, offering a feasible approach to safeguarding food security and human health.

The invention discloses a method for cultivating high-starch potatoes through grafting, and relates to the technical field of potato cultivation. The method comprises the following specific steps: (1) cultivating a high-starch potato stock and a common potato scion; (2) grafting the rootstock and the scion; (3) grafted plant treatment; (4) domestication treatment: carrying out stress treatment on the grafted seedlings for 10-15 days, then transferring the grafted seedlings outdoors for culture, and harvesting tubers; and (5) screening of high-starch germplasm: selecting tubers with high starch content for planting, repeatedly domesticating for 2-3 generations, and harvesting tubers to obtain the high-starch potato germplasm. According to the method, the high-starch potato seedlings and the common potato seedlings are grafted, aeroponic culture management is performed after grafting, domestication treatment is performed, the starch content of the grafted potatoes can be increased, and after multi-generation domestication, the high-starch potato germplasm is obtained through breeding. According to the method, the variety breeding process is shortened, and the problem that the high-starch potato germplasm is not created by adopting a grafting method at present is solved.

The present invention relates to a method for constructing a dry eye cell model based on mitochondrial dysfunction, which is characterized in that the human corneal epithelial cells cultured in vitro are treated by a method of targeted knockout or knockdown of the expression level of TFAM in human corneal epithelial cells, and then a dry eye cell model induced by mitochondrial dysfunction is constructed in a directional manner. The present invention uses targeted knockout / knockdown of the expression of TFAM in human corneal epithelial cells to establish a dry eye cell model that can better and more realistically reflect mitochondrial dysfunction-induced dry eye cell model, which has the characteristics of high model construction success rate, stable model characteristics, and no generation of non-target cell models, and is conducive to the study of the mechanism of downstream mitochondrial dysfunction in the occurrence and development of dry eye or horizontal comparison between treatment schemes.

This invention discloses a plant-derived herbicide and its preparation method, specifically relating to the field of biological weed control. The whole plant of *Coriandrum sativum* is dried, pulverized, and sieved. After ultrasonic extraction with an organic solvent, the extract is concentrated to obtain a paste-like extract. This plant-derived herbicide contains the following raw materials in volume fractions: 10 parts *Coriandrum sativum* extract, 2 parts emulsifier, and 88 parts water. The technical solution of this invention solves the problem of environmental pollution and pesticide residues exacerbated by existing chemical herbicides. Furthermore, the plant-derived herbicide of this invention, after being prepared into a stable formulation, can effectively control annual weeds, achieving a control effect of over 75% on weeds in farmland. The application of this plant-derived herbicide can significantly reduce the amount of chemical herbicides used, thus reducing environmental pollution.

The invention provides a method for obtaining a sugarcane variety with high smut resistance by using a Clerodendron simsii polymer, and relates to the technical field of sugarcane breeding. The method comprises the following steps: hybridizing Yunnan 82-1 (smut resistance) serving as a female parent and Cipmi Yunnan 2017-22 (smut resistance) serving as a male parent to obtain a high-smut-resistance Cipmi hybrid A, hybridizing a high-smut-resistance sugarcane variety CP72-3709 serving as a female parent with A to obtain a hybrid B, hybridizing a high-smut-resistance sugarcane variety CP89-2143 serving as a female parent with the hybrid B to obtain a hybrid C, and hybridizing the hybrid C with Cipmi Yunnan 2017-22 (smut resistance) serving as a female parent with Cipmi Yunnan 2017-22 (smut resistance) serving as a male parent to obtain the high-smut-resistance Cipmi hybrid. And after three times of hybridization, the offspring hybrid C is a high-resistance parent containing two cleft-hand dense consanguinity. According to the method, the hybrid C is hybridized with other high-smut-resistance sugarcane varieties, the high-sugar and high-smut-resistance sugarcane varieties can be obtained, the obtained high-sugar and high-smut-resistance sugarcane varieties are stable in character, and the sugar content and smut resistance of filial generations are remarkably enhanced.

The invention relates to biological medicine manufacturing, in particular to a dexmedetomidine freeze-dried composition and a preparation method thereof.The freeze-dried composition comprises active pharmaceutical ingredients and auxiliary materials, the active pharmaceutical ingredients in unit dosage are smaller than 200 micrograms, and the auxiliary materials comprise a skeleton supporting agent, an adhesive, edible essence and a sweetening agent; the skeleton supporting agent, the adhesive and the sweetening agent are all water-soluble. The freeze-drying composition disclosed by the invention can be disintegrated in the oral cavity, and the defect that the dosage of the taken medicine is greatly changed due to the condition that microgram-level effective component freeze-drying preparations stick to the bottom or fall off residues in the preparation process is overcome. In addition, the freeze-dried composition can be rapidly absorbed in the oral cavity and rapidly take effect, the first-pass effect is reduced, and the bioavailability is improved.

The application belongs to the technical field of biological probes, and relates to a PD-L1 guided specific targeted CTC detection combined with tumor boundary distinguishing biological probe and a preparation method thereof. The PD-L1 guided specific targeted CTC detection combined with tumor boundary distinguishing biological probe comprises a core, a covalent organic framework and a PD-L1 antibody, the core comprises a superparamagnetic nanoparticle, the covalent organic framework is coated on the surface of the core, and the PD-L1 antibody is combined on the surface of the covalent organic framework. The PD-L1 guided specific targeted CTC detection combined with tumor boundary distinguishing biological probe prepared by the application can be transported into the body across the membrane, smoothly enter a PD-L1 positive malignant tumor lesion, and perform Raman imaging to microscopically distinguish the tumor boundary.

The invention belongs to the field of edible mushrooms, and particularly discloses a Morchella sextelata strain Qian DL02 which is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the Morchella sextelata strain is CCTCC NO: M2022899. The invention further discloses application of the Qian DL02 in the fields of food, beverages and the like. The ascotums of the strain Qian DL02 are light brown, the mushroom shape is good, and the commodity value is high; in addition, the strain also has the advantages of short cultivation period, high yield, strong heavy metal chromium stress resistance and the like.

The invention discloses a method and a device for creating heat-resistant potato germplasm through interspecific grafting. The method comprises the following steps: (1) cultivating potato seedlings and tomato seedlings; (2) mutual stock-scion grafting; 3, grafted plant treatment, wherein after grafting is conducted for three weeks, grafting openings are pulled to the positions 15 cm below plate holes, and variable temperature treatment is conducted; (4) potato seed management: cutting off all the grown stolons before temperature-changing treatment, leaving the potatoes after temperature-changing treatment, and harvesting the potatoes at proper time; and (5) heat-resistant domestication: after 5-6 compound leaves grow on the seed potato potted plant in the step (4), performing high-temperature domestication at 38 DEG C in the daytime and 30 DEG C at night in an illumination incubator for 9 days, transferring the heat-resistant plant to outdoor culture, and harvesting tubers. And (6) heat-resistant germplasm screening: repeating the step (5) for two generations, and harvesting tubers. According to the method, the potato germplasm with high heat resistance and genetic stability is obtained, the variety breeding process is shortened, and the problems that a heat-resistant potato germplasm creation technology and heat-resistant varieties are lacked, and potato production is affected by high temperature are solved.

The invention discloses a fresh rehmannia extract, fresh rehmannia glutinosa phenolic acid components, an extraction method and application, and belongs to the field of daily chemical products. The extraction method comprises the following steps: adding ethanol and enzyme to fresh rehmannia glutinosa, extracting twice, and filtering to obtain a filtrate; decompressing and concentrating the filtrate, and then purifying it through a membrane, collecting the clear liquid, and obtaining a fresh rehmannia extract; separating and purifying the fresh rehmannia extract with a macroporous adsorption resin, and obtaining fresh rehmannia glutinosa phenolic acid components. The fresh rehmannia extract and fresh rehmannia glutinosa phenolic acid components extracted by the invention have good antibacterial activity against Staphylococcus aureus and Propionibacterium acnes that cause acne, and also have a significant inhibitory effect on acne muscle inflammation. The invention provides a new natural raw material and method for acne removal and anti-inflammation.

This invention, entitled "A Method for Preparing an Antiepileptic Carbon Dot Suspension," belongs to the field of pharmaceutical technology. It relates to a method for preparing a nanoparticle suspension and provides a carbon dot suspension for treating epilepsy. The raw materials and excipients of the suspension include: carbon dots from human hair, stabilizers, flavoring agents, and preservatives. The suspension of this invention exhibits stable properties, simple excipient composition and preparation process, and excellent taste. The most preferred formulation is: 5g of carbon dots from human hair, 0.125% CMC-Na, 0.05% ethylparaben, and 2.5% sucrose.

The invention discloses an organic water-soluble fertilizer and a preparation process thereof, and relates to the technical field of water-soluble fertilizers. The organic water-soluble fertilizer comprises the following components: 85-105 g / L of reef film extract; 18-34 g / L of live microorganisms; 5-7 g / L of fulvic acid; 34-52 g / L of corn syrup; 47-63 g / L of small-particle diatomaceous earth; 72-88 g / L of birch sap; and water. The organic water-soluble fertilizer of the invention is obtained by using organic resources such as reef film extract, live microorganisms, fulvic acid, corn syrup, and birch sap as main raw materials and processed through a specific process. The fertilizer has comprehensive and balanced nutrients, and the beneficial bacteria contained therein can comprehensively regulate the physiological functions of crops, safely promote the healthy growth of crops, improve soil matrix, inhibit soil-borne diseases, and enhance the soil's ability to retain and supply fertilizers. The organic matter is high, which can promote crop growth and the reproduction of beneficial live bacteria. The marine extracted active substances contained in the fertilizer, through the synergistic effect with other components, stimulate the physiological activity of crops and improve resistance.

The invention discloses a method for preparing dendrobium officinale primary pulp while fresh, and belongs to the technical field of health food development. The step of preparing the dendrobium officinale primary pulp while the dendrobium officinale is fresh comprises the following steps: mixing crushed fresh dendrobium officinale stalks with an extracting solution, pulping, then gelatinizing, and filtering to obtain filtrate, namely the dendrobium officinale primary pulp; the extracting solution is an aqueous solution in which amino acid and citric acid are dissolved. According to the preparation method, the aqueous solution containing amino acid and citric acid is used as the extracting solution, the fresh dendrobium officinale stems are adopted to prepare the dendrobium officinale primary pulp while being fresh, the yield of effective components in dendrobium officinale is increased, no preservative or stabilizer is added in the process, the components are natural and pure, and the primary pulp is good in oxidation resistance, stable in character and fresh and sweet in taste.

The invention discloses a bifidobacterium longum subsp. Longum i772 metagen product with a fat reducing function, a composition and application, and belongs to the field of microbial engineering. Wherein the metabiotic product comprises an inactivated bifidobacterium longum subsp. Longum i772, an inactivated bifidobacterium longum subsp. The composition is prepared from the following raw materials in parts by weight: 0.0002 to 10 parts of bifidobacterium longum subsp. Longum i772 bacterial powder, 0.015 to 0.06 part of L-carnitine, 0.5 to 3 parts of long-chain inulin and 1 to 5 parts of isomaltooligosacharide. The bifidobacterium longum subsp. Longum i772 provided by the invention can achieve the effects of promoting digestion and metabolism and losing weight by increasing the abundance of colabacillus and ruminococcus in intestinal tracts. The method is suitable for preparing milk powder or other foods and health-care products with a fat-reducing function.

The invention discloses a method for preparing aluminum chloride by leaching fly ash with hydrochloric acid under the assistance of chlorine, and belongs to the technical field of resource utilization of fly ash and preparation of aluminum oxide. The method comprises the following steps: utilizing hypochlorous acid with strong oxidizing property generated when chlorine is dissolved in an aqueous solution to promote decomposition of fly ash, promoting aluminum oxide components in the fly ash to be dissolved into the solution and exist in the solution in the form of AlCl3, simultaneously or crosswise introducing hydrogen into the solution, and enabling the chlorine and the hydrogen to react in a solution environment, so as to obtain the aluminum oxide in the fly ash. And chemical energy released during complexing of chlorine and hydrogen is directly converted into chemical energy required by decomposition of fly ash. According to the method, the hydrochloric acid concentration, the leaching temperature and the leaching pressure required in the leaching process can be reduced. And the corrosion problem of leaching equipment is relieved. And meanwhile, the energy cost consumed for maintaining a relatively high leaching temperature is reduced.

The present application relates to a kind of yunnan ganoderma strain HL45 and its cultivation method and application, belong to the field of microbial technology.The strain has been preserved in Guangdong Provincial Microbial Culture Collection Center on September 11, 2024, and the preservation number is GDMCC No:65119.The present application yunnan ganoderma strain HL45 fruiting body presents single or multi-branch, cap purple brown, flesh white, and stable in character;The content of triterpenoid ganoderol F and glucosan polysaccharide is very high, which can be used for preparing antitumor drugs.In addition, the fruiting body of the strain has strong mushroom aroma, and the quality of the whole mushroom is good.The average growth cycle of cultivation bag is 26d, the mycelial growth rate is 0.78cm / d, the average yield is 16.62g / bag, the highest biological efficiency reaches 10.26%, the cultivation period is about 4-5 months, and it is suitable for cultivation in high-altitude areas above 2000m, with high ornamental and application value.

The invention provides an antioxidant health-care beverage and a preparation method thereof. The health-care beverage is prepared from medicinal and edible traditional Chinese medicines including honeysuckle, chicory, day lily, pawpaw and folium cortex eucommiae through extraction, flocculation and purification. On the basis, the invention also verifies the in-vitro antioxidant activity of the beverage. The health-care beverage prepared by the method disclosed by the invention is scientific in compatibility, simple to operate and good in antioxidant effect. Compared with traditional antioxidant drugs and health-care products, the traditional Chinese medicine composition focuses on medicinal and edible traditional Chinese medicines, can remove harmful free radicals generated by oxidative stress, is high in safety, remarkably reduces the bitter taste of the traditional Chinese medicines, expands the range of adaptive people, and provides a new thought for product development of the medicinal and edible traditional Chinese medicines.

The invention belongs to the technical field of high polymer materials, and discloses a preparation method of nanoscale hydrophobic starch, the nanoscale hydrophobic starch, application of the nanoscale hydrophobic starch and reinforced styrene-butadiene rubber based on the nanoscale hydrophobic starch. According to the preparation method disclosed by the invention, starch is firstly subjected to nanocrystallization treatment by adopting a nanoprecipitation method and then is subjected to hydrophobic modification, so that the nano-scale hydrophobic starch is prepared. The nanoscale hydrophobic starch disclosed by the invention is applied to reinforcement of styrene-butadiene rubber, and the nanoscale crystalline starch is dispersed in the styrene-butadiene rubber in a nanoscale manner, has a micro-nano island structure, and improves the modulus and strength of a reinforcement body; through high-efficiency hydrophobic modification, the interface strength is further improved, the water absorption of the starch is reduced, and the defect that the performance of the butadiene-styrene rubber is reduced due to water absorption swelling in the use process of the starch is overcome; the mechanical properties of the reinforced styrene-butadiene rubber are obviously improved, the tensile strength is improved by 110% or above, the elongation at break is improved by 59%, and the stress at definite elongation of 100% and the stress at definite elongation of 300% are also obviously improved.

The invention discloses a KASP marker primer combination for detecting the gloss character of watermelon peel and application of the KASP marker primer combination, and belongs to the technical field of watermelon breeding molecular biology. The KASP labeled primer combination comprises an upstream primer 1, an upstream primer 2 and a downstream primer 1, the nucleotide sequence of the upstream primer 1 is as shown in SEQ ID NO. 1; the nucleotide sequence of the upstream primer 2 is as shown in SEQ ID NO. 2; and the nucleotide sequence of the downstream primer 1 is as shown in SEQ ID NO.3. According to the KASP marker primer combination, a KASP molecular marker Cla27993083 is used as a detection target. When the primer combination is applied to amplification, the luster character of the watermelon peel can be accurately and quickly identified, and a new technical path is provided for screening and identifying a watermelon material with the luster peel and breeding a watermelon variety with the luster peel.

The invention relates to the technical field of microalgae biology, in particular to a chlorella pyrenoidosa mutant strain and application thereof. According to the invention, a mutagenesis screening mode is adopted to obtain a chlorella pyrenoidosa mutant strain which is named as RMCP012, the chlorella pyrenoidosa mutant strain can still grow quickly after being continuously cultured for one year and is stable in character, and the protein content of the chlorella pyrenoidosa mutant strain is equivalent to that of a wild type chlorella pyrenoidosa mutant strain. Meanwhile, the algal strain of the mutant strain is light green, the decoloring process of industrial production of algal source protein is greatly reduced, the industrial cost is reduced, and the mutant strain is a high-quality algal strain capable of being used for industrial production of protein. The invention further relates to a heterotrophic culture mode of the chlorella pyrenoidosa, so that the chlorella pyrenoidosa can grow quickly, and protein with a relatively high level can be accumulated.

The invention discloses an ornamental shallot planting method, and belongs to the technical field of plant rapid propagation, and the method comprises the following steps: (1) explant disinfection; (2) callus induction; (3) differentiation culture; (4) multiplication culture; (5) rooting. According to the ornamental shallot planting method, the reproduction rate of ornamental shallots in each link from explant treatment to callus induction and adventitious bud induction to adventitious bud proliferation and finally to rooting is very high, the characters are stable, the excellent characters of female parents are kept to the maximum extent, and the ornamental shallots have good economic benefits. The invention establishes a good rapid propagation mode of the ornamental green onion, lays a foundation for research work such as new variety cultivation and genetic improvement on the variety by using a plant biotechnology in future, and also provides technical guidance and reference for establishment of a tissue culture rapid propagation technology of other plant varieties.

The invention relates to an application of an SlCLE3 gene and polypeptide thereof in improvement of salt tolerance of tomatoes. Specifically, the nucleotide sequence of the SlCLE3 gene is as shown in SEQ ID NO: 1. According to the invention, an overexpression vector of the SlCLE3 gene is constructed, and an agrobacterium tumefaciens-mediated genetic transformation method is utilized to obtain a transgenic tomato homozygous strain overexpressed by the SlCLE3. Under the condition of salt stress, the transgenic line shows significantly enhanced salt tolerance, specifically, active oxygen accumulation is reduced, cell damage is reduced, photosynthetic indexes are improved, and relative conductivity is reduced. In addition, the damage of salt stress to tomato plants can be effectively relieved by exogenously applying the pCLE3 polypeptide. The invention provides a new gene resource and a breeding method for tomato salt-tolerant breeding.