439 results about "Lycium barbarum polysaccharide" patented technology

The invention provides a food for reducing blood sugar and blood fat, which is prepared from full tartary buckwheat flour, soybean protein powder, oat bran powder, Lycium barbarum polysaccharides, timosaponin, ganoderma lucidum polysaccharide, vitamins and edible calcium carbonate. The preparation method comprises the processes of raw material weighting, raw material mixing, crushing and screening, forming tabletting or granulation, baking, curing, standing, cooling and packaging to prepare the food for reducing blood sugar and blood fat. The food has scientific formula, wide raw materials, convenient taking by the patients and easy promotion.

The invention provides a method for efficiently extracting lycium barbarum polysaccharide. The method comprises the steps: adding lycium barbarum into an extraction kettle, injecting preheated deionized water to the extraction kettle at the flow speed of about 70mL/min by using a pump, controlling the liquid to solid ratio to be 10:1 to 30:1mL/g, simultaneously extracting the lycium barbarum polysaccharide at an extraction phase by using synergistic ultrasonic and subcritical water methods after heating to the preset temperature (larger than or equal to 100 DEG C), controlling the extraction pressure to 3-7MPa, the extraction temperature to 100-130 DEG C and the extraction time within 20-80min, and discharging an extracting solution after the extraction is ended, wherein the lycium barbarum is dried to the constant weight, the frequency of the ultrasonic is 18.5Hz, and the ultrasonic power is 100-140W; and after the extracting solution is filtered and subjected to vacuum concentration, settling for 12h by using 95% ethanol, removing a supernatant liquid and drying in vacuum to obtain the lycium barbarum polysaccharide. The yield of the lycium barbarum polysaccharide extracted by using the method is higher than the yields of lycium barbarum polysaccharides extracted by using a hot water extraction method, an ultrasonic extraction method and a subcritical water extraction method, and the antioxidant activity of the lycium barbarum polysaccharide extracted by using the method is stronger than the antioxidant activities of the lycium barbarum polysaccharides obtained by using the hot water extraction method, the ultrasonic extraction method and the subcritical water extraction method. Therefore, the method can lay the foundation for the industrial production and pharmacological research of the lycium barbarum polysaccharide.

The invention relates to a preparation method of RG-I type lycium barbarum pectin with anti-aging activity. The method comprises the following steps: (1) water is added into lycium barbarum, and the mixture is subjected to microwave-ultrasonic assisted extraction, filtering and concentration, such that a concentrate liquid is obtained; (2) anhydrous ethanol is added into the concentrate liquid, and the materials are well mixed; the mixture is allowed to stand and is subjected to centrifugation, such that a precipitation product is obtained; (3) the precipitation product is re-dissolved, and the solution is subjected to centrifugation and drying, such that lycium barbarum polysaccharide is obtained; (4) lycium barbarum polysaccharide subjected to DEAE-cellulose column chromatography separation, and is eluted; and dialysis desalination and lyophilization are carried out, such that lycium barbarum neutral polysaccharide LBP-N and lycium barbarum acidic polysaccharide LBP-A are respectively obtained; (5) lycium barbarum acidic polysaccharide LBP-A is subjected to Sepharose CL-6B gel filtration column chromatography separation, and is eluted; dialysis desalination and lyophilization are carried out, such that lycium barbarum acidic polysaccharides LBP-A-1 and LBP-A-2 are obtained; (6) lycium barbarum acidic polysaccharide LBP-A-1 is subjected to DEAE-Sepharpse Fast Flow anion exchange column chromatography purification; continuous gradient elution is carried out with a 0-0.5 mol/L NaCl aqueous solution; and dialysis desalination and lyophilization are carried out, such that the anti-aging RG-I type lycium barbarum pectin pure product is obtained. The method provided by the invention has the advantages of simple operation and high extraction rate

The invention discloses a food additive for expelling toxin and nourishing skin, which is obtained by taking maize peel, wheat bran, lecithin, seabuckthorn, red dates, medlars, coix seeds, chrysanthemum, black sesame, yam, pawpaw, lucerne, mulberry, fructus cannabis, fingered citron, brush cherry seeds, diamonds, jujube kernels, radix puerariae, xylooligosaccharide and colla corii asini as raw materials according to a method of: drying and crushing the maize peel to obtain zein fiber after the maize peel is subjected to alkali treatment to remove protein and is subjected to acid treatment to remove starch; drying and crushing the wheat bran to obtain wheat fiber after the wheat bran is subjected to acid treatment to remove starch and is subjected to alkali protease treatment to decompose protein; using water to extract lycium barbarum polysaccharides and jujube date polysaccharides from the crushed medlars and the pitted red dates; using a supercritical CO2 extraction method to extract seabuckthorn oil from the seabuckthorn; crushing other raw materials; and mixing the materials, stirring the mixture, and sieving the mixture to obtain a finished product of the food additive. The food additive has the unique effect which cannot be substituted by medicaments, has a simple manufacturing method, and has large market potential.

The present invention discloses a synbiotic feed additive, and a preparation method and applications thereof. The synbiotic feed additive comprises: 1 ~ 5 parts by weight of, preferably 2-4 parts by weight of, more preferably 3-4 parts by weight of, and most preferably 3.5 parts by weight of at least one selected from chitosan oligosaccharide, chitosan and algal oligosaccharide; 2-10 parts by weight of, preferably 3 to 9 parts by weight of, more preferably 4-8 parts by weight of, and most preferably 5.5 parts by weight of at least one selected from astragalus polysacharide, and lycium barbarum polysaccharides hawthorn flavone; and 100 parts by weight of probiotics composition which is composed of bacillus subtilis, bacillus coagulans and clostridium butyricum at a weight ratio of 1-3:1-3:1-3, preferably 3:3:3, relatively preferably 3:2:2, more preferably 3: 2:1, and most preferably 3:1:1. Compared with existing synbiotic feed additives, the synbiotic feed additive of the present invention is easier to digest and absorb, and can significantly improve animal immunity and animal health, increase feed utilization, lower feed conversion rates, promote reproduction, improve production performance, improve meat quality and improve breeding environment.

The invention relates to a lycium ruthenicum murr anthocyanin chewable tablet which is prepared from the following components in parts by weight of 50-500 parts of lycium ruthenicum murr anthocyanin, 30-300 parts of peach flower kaempferol, 10-100 parts of rhizome of conic gymnadenia and 50-500 parts of lycium barbarum polysaccharide according to a conventional method comprising the steps of sequentially granulating, drying at the temperature of 40-50 DEG C, finishing, and tabletting under the condition that the pressure is 80-100kN. The lycium ruthenicum murr anthocyanin chewable tablet has the advantages that raw materials are easily available, industrial implementation is realized easily, and a human body can maximally utilize and absorb plant extracts.

The invention relates to an extraction method of high cell apoptosis induction active lycium barbarum polysaccharide. The extraction method comprises using the fruit of Chinese wolfberry to serve as a raw material, obtaining lycium barbarum coarse polysaccharide through extraction, adopting an ultrafiltration membrane with 30K of molecular weight cutoff to separate the lycium barbarum polysaccharide, collecting percolate, using an ultrafiltration membrane with 10K of molecular weight cutoff to separate, collecting cutoff liquid 1, using an ultrafiltration membrane with 40K of molecular weight cutoff to separate, collecting percolate 1, conducting vacuum concentration and freeze drying on the cutoff liquid 1 and the percolate 1, and obtaining the high cell apoptosis induction active lycium barbarum polysaccharide. The extraction method uses a water extraction method to extract the lycium barbarum polysaccharide, removes protein, then adopts an ultrafiltration technique to grade and separate the polysaccharide, adopts the ultrafiltration membrane with a plurality of molecular weight cutoffs to repeatedly separate, overcomes shortcomings of being complex in column chromatography operation and unfavorable for industry or production and the like, is high in efficiency, low in cost, simple in operation, soft in condition, short in production phase and the like and provides a new thought for development of an active compound and industrialized application.

The invention relates to a preparation method of lycium barbarum polysaccharide. The preparation method comprises the following steps: adding water to soak lycium barbarum; pulping; removing seeds; placing lycium barbarum pulp in a circular ultrasonic extraction machine for ultrasonic extraction; carrying out solid-liquid separation; concentrating the filtrate; adding a certain amount of ethanol to precipitate polysaccharide; purifying the polysaccharide by resin to prepare a lycium barbarum polysaccharide product. The preparation method provided by the invention solves the problems that conventional extraction of lycium barbarum polysaccharide by hot water is long in time consumption, high in energy consumption and low in yield, degradation to polysaccharide by high temperature, the biological activity of polysaccharide is destroyed and the like by means of advantages of a circular ultrasonic extraction technology so as to realize the purpose of quickly and efficiently producing lycium barbarum polysaccharide.

The invention provides a NK (natural killer) cell culture medium and a culture method of a NK cell, wherein the NK (natural killer) cell culture medium comprises the following components in parts by weight: 83 to 93 parts of serum-free basal culture medium; 4 to 6 parts of plasma; 0.5 to 1.5 parts of interleukin-2; 0.5 to 1.5 parts of anti-CD3 monoclonal antibody; 1 to 5 parts of insulin-like growth factor 1; 1 to 3 parts of lycium barbarum polysaccharide. According to the NK (natural killer) cell culture medium provided by the invention, the safety is better, and the NK cell cultured by the culture medium with the components can quickly and stably proliferate, and has better killing activity to tumor cell. Experimental results show that, after using the NK cell culture medium provided by the invention for culturing the NK cell for two weeks, the proliferation times soars almost fiftyfold; the killing activity to K562 reaches 91 percent at effector-target ratio being 40: 1.

The invention discloses a preparation method of lycium barbarum polysaccharides and belongs to the technical field of food processing. The preparation method of lycium barbarum polysaccharides comprises steps as follows: sieving, impurity removal, drying, crushing, separation, supercritical CO2 extraction, soaking, enzymolysis, centrifugation, desalination, four-level ultrafiltration, reverse osmosis concentration, freeze sublimation drying, low-temperature crushing, packaging and obtaining of a finished product, namely, the lycium barbarum polysaccharides. The preparation method of the lycium barbarum polysaccharides has the characteristics of simple process, high operability and the like. The lycium barbarum polysaccharides are extracted in an ultrafiltration and reverse osmosis concentration combined manner, the rejection rate of the lycium barbarum polysaccharides can reach 94.3%, and the largest content of the lycium barbarum polysaccharides reaches 65% or above. According to the lycium barbarum polysaccharides prepared with the method, the anti-oxidation index of the lycium barbarum polysaccharides is tested to be larger than or equal to 150,000, and the highest index can reach 163,000; 100 times or more of experiment researches prove that the technical data tend to be stable, the product quality is reliable, and industrial and large-scale production is easy to realize.

The invention belongs to the technical field of lycium barbarum polysaccharide extraction, and provides an ultrasonic extraction method of lycium barbarum polysaccharide. The method comprises the following steps of: accurately weighing 5g of lycium barbarum dry powder, placing in a conical flask and adding 150mL of distilled water; performing ultrasonic extraction twice, filtering, combining the filtrates, precipitating with 95% ethanol and standing for 24 hours before suction filtration; washing the obtained solid matter sequentially with 95% ethanol, absolute ethyl alcohol and acetone; and performing vacuum drying at 50 DEG C to obtain the coarse powder of solid matter lycium barbarum polysaccharide, wherein the best technological conditions of the ultrasonic extraction of lycium barbarum polysaccharide are as follows: the ultrasonic material-liquid ratio is 1:30, the power is 90W, the time is 30min, and the extraction is conducted twice; and moreover, the extraction technology is simple, the usage amount of the extraction solvent is small, the yield is high, the cost is low, and a great value in popularization and application is realized.

The invention discloses a method for preparing black rice enzymatic hydrolysate. The preparing method comprises the following steps of, conducting extrusion expansion and grinding to black rice materials to prepare puffed black rice micro powder; adding the black rice micro powder with purified water to prepare puffed black rice paste; conducting liquidation to the puffed black rice paste by adopting alpha-amylase; after liquidation, conducting combined enzymolysis to the puffed black rice paste by cellulase and glucoamylase to prepare black rice enzymatic hydrolysate. Prepared black rice enzymatic hydrolysate helps not only with digestive absorption of black rice products but also with effective release and reservation of polyphenol substances black rice cortex. Prepared black rice enzymatic hydrolysate and an auxiliary material such as lycium barbarum juice are compounded into black rice polyphenol compound beverage respectively with the weight percentage of 60%-80% of black rice enzymatic hydrolysate and the weight percentage of 20%-40% of lycium barbarum juice, and black rice polyphenol and lycium barbarum polysaccharide are enriched in the black rice polyphenol compound beverage.

The invention relates to the field of biological medicines, in particular to a method for enhancing activity of CIK (cytokines-induced killer) cells through traditional Chinese medicine induction. The method comprises the step of adding rhizoma atractylodis macrocephaiae and lycium barbarum extracts into a culture fluid for the CIK cells, wherein the rhizoma atractylodis macrocephaiae and lycium barbarum extracts are rhizoma atractylodis macrocephaiae polysaccharide and lycium barbarum polysaccharide. According to the method, the activity of the CIK cells in the aspects of proliferating, tumor killing and immunity can be activated; the in-vitro tumour killing effect of the CIK cells is improved, and meanwhile, a large number of CIK cells can be obtained, so that the cell culture time is shortened, and the immunological activity of the CIK cells can also be enhanced; the enhanced CIK cells prepared by utilizing the method can be used for treating tumour diseases.

The invention relates to an instant ginger decoction powder and instant ginger decoction. The instant ginger decoction powder and the instant ginger decoction are characterized in that: the instant ginger decoction powder is prepared by: adding 10-30 parts by weight of a mixture of potato starch and soluble starch with a weight ratio of 1:1-1:3, 1-5 parts by weight of water-soluble dietary fiber, 5-10 parts by weight of maltitol, 1-5 parts by weight of collagen powder and 1-5 parts by weight of lycium barbarum polysaccharide into each 100 parts by weight of ginger juice, mixing uniformly for compounding, homogenizing, performing quick-freezing processing and vacuum freeze drying. The ginger juice is a filtrate obtained by: cleaning ginger, removing impurities, adding water and juicing, then adding cellulase and pectase into a mixture obtained by juicing, keeping warm at 30-50 DEG C for 10-50 min and filtering. The instant ginger decoction powder is capable of keep the ginger flavor and is fast in dissolving speed, and the preparation technology is simple and convenient.

The present invention relates to lick bricks with interaction effects of combining nutrition and improving immunity for cattle and sheep and a technology thereof. The lick bricks contain the following components: lycium barbarum polysaccharides, astragalus polysaccharides, methionine zinc, yeast selenium, sodium bicarbonate, magnesium oxide, corn bran, edible salt and molasses. The lycium barbarum polysaccharides, astragalus polysaccharides, methionine zinc, yeast selenium, sodium bicarbonate, magnesium oxide and corn bran are mixed, the mixture is stirred, and the stirred mixture is superfinely crushed to obtain a first mixture; in the first mixture, the edible salt is added and the materials are stirred to obtain a second mixture; the molasses at 70-150 parts by weight are heated at a temperature of 80-100 DEG C to prepare syrup; and the second mixture and the syrup are uniformly mixed, the mixture is pressed and shaped to obtain the lick bricks. The lick bricks are used for improving immunity and production performance of animals, particularly the immunity and production performance of the cattle or sheep.

The invention relates to a lycium barbarum polysaccharide lozenge and a preparation method thereof. The lozenge is prepared from the following components in parts by weight: 1-50 parts of lycium barbarum polysaccharide, 5-30 parts of microcrystalline celluloses, 1-15 parts of citric acid, 5-60 parts of sugar powder, 0.1-3 parts of a de-molding agent and proper amount of binding agent. The preparation process comprises the following steps: respectively crushing lycium barbarum polysaccharide and all the auxiliary materials, sieving by a sieve of 100 meshes, uniformly mixing, preparing a soft material, pelletizing, drying, granulating and tabletting to obtain the lycium barbarum polysaccharide lozenge. The lozenge has the effects of aging resistance, fatigue resistance, blood sugar reduction, tumor resistance, blood fat resistance, oxidization resistance, human body immunity adjustment and the like.

The invention relates to a novel method for preparing a Qinghai Qaidam lycium barbarum polysaccharide in an extraction and separation manner. The method comprises the following steps: (1) washing fresh Qinghai Qaidam lycium barbarum fruits taken as raw materials with pure water for multiple times and then removing impurities so as to obtain treated lycium barbarum; (2) soaking the treated lycium barbarum in the pure water at a normal temperature and carrying out smashing so as to obtain the smashed lycium barbarum; (3) carrying out ultrasonic extraction by adding the pure water into the smashed lycium barbarum so as to obtain an extraction liquid; (4) filtering the extraction liquid with a filter cloth so as to respectively obtain a filtrate and filter residues; (5) centrifuging the filtrate so as to respectively obtain a supernate and residues; (6) feeding the supernate to pass through a nonpolar macroporous resin and then cleaning a resin column with the pure water so as to obtain a polysaccharide eluent; (7) removing granular impurities from the polysaccharide eluent via a ceramic membrane so as to obtain a transparent liquid; (8) intercepting the transparent liquid with an organic ultrafiltration membrane so as to obtain an intercepting liquid, carrying out freeze drying on the intercepting liquid to reach a constant weight so as to obtain a brown yellow Qaidam lycium barbarum polysaccharide powder product. The method is simple, convenient, rapid and low in cost.

The invention discloses lycium ruthenicum anthocyanin soft capsules and a preparation method thereof. The soft capsules comprise contents and shells, wherein the contents are composed of lycium ruthenicum anthocyanin, lycium barbarum polysaccharide and wolfberry seed oil; the shells are composed of gelatin, water and glycerinum. The invention further discloses the preparation method of the soft capsules. The preparation method is simple, the produced lycium ruthenicum anthocyanin soft capsules are good in stability, convenient to take and extremely high in nutritive value.

The invention discloses a health-care product with a blood glucose reducing function. The health-care product is prepared from the following raw material components in parts by weight: 10-20 parts of semen coicis extracts, 10-15 parts of euonymus alatus extracts, 8-10 parts of astragalus polysaccharides, 5-8 parts of corn stigma aqueous extracts and 3-5 parts of lycium barbarum polysaccharide. The extracts of all the components are prepared according to a composite formula in which semen coicis, euonymus alatus, astragalus, corn stigma and lycium barbarum which all have a better blood glucose reducing effect are scientifically selected, so that the blood glucose reducing components are enriched, the effect of the health-care product regulating diabetes can be enhanced, and the health-care product can also be used for effectively preventing, treating and relieving the happen and development of diabetic complications and is suitable for being taken by diabetic patients for a long term.

The invention relates to the technical field of biological extraction and separation in plant natural products, in particular to an extraction process of lycium barbarum polysaccharide. The process comprises the following steps of (1) screening and impurity removal: screening lycium barbarum fruits; removing impurities and diseased fruits; (2) dewatering and drying: putting the lycium barbarum fruits at 50 DEG C to be subjected to vacuum drying for 24h; (3) crushing and separation: crushing the dried lycium barbarum fruits by a coarse particle crushing machine; then, passing the lycium barbarum fruits through a three-stage sieve to separate pulp powder and lycium barbarum seeds, wherein the crushing and the sieving are performed under normal temperature condition; (4) extraction and enzymolysis: adding petroleum ether into the lycium barbarum powder for extraction; performing vacuum drying on degreased powder at 60 DEG C to reach the constant weight; adding the degreased powder into acomplex enzyme solution for extracting; then, putting the degreased powder into a boiling water bath to be heated for enzyme deactivation; performing suction filtering after cooling; concentrating thesolution; (5) adding 4 times of volume of absolute ethyl alcohol into the concentrated solution; performing still standing for 12h at 4 DEG C for alcohol precipitation; performing centrifugation for15min under the condition of 4200rpm; taking precipitates; washing the precipitates by absolute ethyl alcohol for three times; performing drying to obtain coarse polysaccharide.

The invention relates to a preparation method of fruit juice, in particular to a preparation method of lycium barbarum raw juice capable of being preserved for a long time, which comprises the following steps: screening lycium barbarum fresh fruit, disinfecting, sterilizing, juicing, grinding, homogenizing, sterilizing at high temperature and filling. The preparation method of the invention is characterized by treating the lycium barbarum fresh fruit by food grade chemical bactericide and initially sterilizing by ultraviolet rays before juicing, adding vitamin C after juicing, and adopting high temperature sterilization and sterile filling, thereby prolonging the storage time of the lycium barbarum raw juice at room temperature, and slowing down the reduction of the contents of main biological active components such as lycium barbarum polysaccharide, betaine and beta-carotene in the lycium barbarum raw juice. Especially, by adding the vitamin C, the preservation effect of the beta-carotene is obviously improved, the lycium barbarum raw juice can be stored for 18-24 months at ordinary temperature, and the problem of processing in the concentration mature period of the lycium barbarum fresh fruit is solved. The technical scheme of the invention has convenient operation, green and energy saving, solves the technical problem in the deep processing course of the lycium barbarum product, and is suitable for popularization and use.

The invention relates to a complex enzyme microwave extraction method for optimizing lycium ruthenicum polysaccharide by utilizing a response surface method. The method comprises the following steps: firstly, sample preparation: preparing black medlar fruits into black medlar fruit powder, and then performing reaction extraction according to different ratios of material to solvent, different soak time, different microwave power and different extraction time to obtain black fruit medlar polysaccharide extract power with different microwave power; (2) measuring the polysaccharide content in each extracting solution by using a phenol-sulfuric acid method; (3) experimental design and statistical analysis: firstly, single factor experiment; and secondly, response surface method optimization design: according to the result of the single factor experiment, selecting four factors such as microwave power, extraction time, soak time and ratio of material to solvent with obvious influence on polysaccharide extraction effect to establish a pluralistic quadratic regression equation; (4) experimental result analysis and optimization: utilizing Design Expert 8.0 software to perform graphical analysis according to the pluralistic quadratic regression equation so as to obtain a response surface of the regression equation and a contour map thereof. The method is simple in operation and high in extraction efficiency.

The invention discloses multidimensional quick-acting medlar powder and a production method of the multidimensional quick-acting medlar powder. The multidimensional quick-acting medlar powder is obtained by performing cleaning, softening, crushing, ultrasonic extraction (water) and centrifugal separation on dry medlar, respectively extracting flavone, glycine betaine and lycium barbarum polysaccharide, then mixing and blending the dry medlar and enzymolyzed medlar juice, adding a drying adjuvant additive and performing uniform stirring, homogenization, concentration, sterilization, spray-drying, screening, detection and package. The multidimensional quick-acting medlar powder is light red, has a special smell of medlar, tastes slightly sweet, has no peculiar smell and contains the following components in percentage by weight: 8-12 percent of lycium barbarum polysaccharide, 1-3 percent of flavone, 1-10 percent of glycine betaine and the balance of medlar powder. According to the production method, the contents of the flavone, the glycine betaine and the lycium barbarum polysaccharide in the medlar powder are increased, and the flavor of fruit powder is improved; a product is convenient to carry, has rich nutrition and has a wide market prospect.

The invention relates to a nanoparticle gene delivery system of cationized lycium barbarum polysaccharides, in particular to a gene delivery system of lycium barbarum polysaccharide-DNA (deoxyribonucleic acid) plasmids modified by using an aminated compound. The molecular weight distribution of lycium barbarum polysaccharides is 20-40KD, wherein the mass ratio of the cationized lycium barbarum polysaccharides to the DNA plasmids is (1-200):1; the particle size of a cationized lycium barbarum polysaccharide-DNA plasmid compound is 351-414 nanometers; and the aminated compound is spermine, ethylene diamine or polyethylene imine with the number average molecular weight of 600-2,000Da. An electrophoresis experiment and a stem cell transfection experiment indicate that three types of cationized lycium barbarum polysaccharides have good DNA plasmid combining functions and genetic transmission expression functions. Meanwhile, an extensive application basis is laid for the gene delivery system of the cationized lycium barbarum polysaccharides by using an appropriate molecular particle size, biodegradability and immunogenicity resistance.