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The Kjeldahl method or Kjeldahl digestion ([ˈkʰɛltæːˀl]) in analytical chemistry is a method for the quantitative determination of nitrogen contained in organic substances plus the nitrogen contained in the inorganic compounds ammonia and ammonium (NH₃/NH₄⁺). Without modification, other forms of inorganic nitrogen, for instance nitrate, are not included in this measurement. This method was developed by Johan Kjeldahl in 1883.
A rapid detection method for protein in feed based on wet chemistry
PendingCN122130632APreparing sample for investigationColor/spectral properties measurementsLinear regressionProtein concentration
This invention discloses a rapid detection method for protein in feed based on wet chemistry. The method includes the following steps: (1) mixing the feed sample with the sample extraction buffer, shaking to extract, centrifuging, and taking the supernatant; (2) adding the working solution of the protein standard and the supernatant to the diquinoline formic acid working solution, incubating, and measuring the absorbance value at a wavelength of 562 nm using an ELISA reader to obtain the absorbance values of the protein standard and the supernatant; plotting a standard curve with the concentration of the protein standard as the abscissa and the absorbance value as the ordinate, and obtaining a linear regression equation; substituting the absorbance value of the supernatant into the linear regression equation to calculate the protein concentration in the supernatant, and then calculating the protein content in the feed sample based on the extraction buffer volume in step (1). This invention achieves a high degree of consistency between the detection results and the Kjeldahl nitrogen determination method, while significantly shortening the detection time of a single sample to less than 1 hour.
Owner:CHINA AGRI UNIV