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Method for extracting protein from cereal fine powder

A technology of protein and fine powder, which is applied to grains that are not easy to extract protein, to separate and extract protein from grain fine powder, and in the field of protein separation and extraction technology, which can solve the problems of difficult protein separation and high-purity protein, and achieve simple operation Easy to control, low cost, no environmental pollution

Inactive Publication Date: 2009-05-20
甘肃奇正实业(集团)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large number of disulfide bonds in rice protein, the protein structure is compact; and highland barley powder contains a large amount of amylopectin, which exists in a bonded state with highland barley protein, and there are a large number of disulfide bonds between highland barley protein and peptide, making the protein Difficult to separate, it is not easy to obtain high-purity protein by conventional extraction methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, the process of extracting protein from highland barley fine powder

[0021] ①Crush the highland barley grains into fine powder, pass through a 100-mesh sieve to obtain the highland barley fine powder;

[0022] ② Mix highland barley powder and water at a mass ratio of 1:6 to 1:10, and stir evenly; adjust the pH value to 6.0 to 6.4 with 3mol / L sodium hydroxide solution, and add 1% to 2% of the powder mass Calcium chloride, fully stirred to make highland barley powder slurry;

[0023] ③Put the highland barley powder slurry in a heater, heat and gelatinize at 40°C-50°C for half an hour, stir well, and gelatinize completely; when the temperature rises to 60-70°C, add medium-temperature α- Amylase liquefies for 1 hour to completely disperse the starch;

[0024] ④Reduce the temperature to 50-55°C, add 2‰ of fine powder glucoamylase to saccharify for 30 minutes; decanter centrifuge for 10 minutes to obtain starch syrup and solid crude protein slag; save the star...

Embodiment 2

[0027] Embodiment 2, the process of extracting protein from highland barley fine powder

[0028] ①Crush the highland barley grains into fine powder, pass through a 100-mesh sieve to obtain the highland barley fine powder;

[0029] ② Mix highland barley powder and water at a mass ratio of 1:6 to 1:8, and stir evenly; use 3mol / L sodium hydroxide solution to adjust the pH value to 6.0 to 6.4, and add 2% to 3% of the powder mass Calcium chloride, fully stirred to make highland barley powder slurry;

[0030] ③Put the highland barley powder slurry in a heater, heat it to 70-80°C, and gelatinize for half an hour (stir fully to make it gelatinize completely); raise the temperature to 80°C, add high-temperature α-starch with a quality of fine powder of 2‰ Enzyme and 0.5‰ amylose, liquefied for 1 hour to completely disperse and liquefy the starch; lower the temperature to 55°C, add 2‰ glucoamylase, and saccharify for 30 minutes; centrifuge in a decanter for 10 minutes to obtain Combin...

Embodiment 3

[0033] Embodiment 3, the process of extracting protein from highland barley fine powder

[0034] ①Crush the highland barley grains into fine powder, pass through a 100-mesh sieve to obtain the highland barley fine powder;

[0035] ②Mix the highland barley powder and water at a mass ratio of 1:6, and stir evenly; use 3mol / L sodium hydroxide solution to adjust the pH value to 6.0-6.4, add calcium chloride with 2% of the powder quality, and stir well, Made into highland barley powder slurry;

[0036] ③ Put the highland barley powder slurry in a heater, heat it to 60-70°C, gelatinize for half an hour, stir well to make it gelatinize completely; raise the temperature to 80°C, add high-temperature α-amylase with a quality of fine powder of 2‰ and 0.5‰ amylose, liquefied for 1 hour to completely disperse the starch; lowered the temperature to 55°C, added 2‰ glucoamylase, and saccharified for 30 minutes; centrifuged with a decanter for 10 minutes to obtain starch syrup with solid cr...

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Abstract

The invention provides a method for extracting protein from grain finemeal, which comprises the steps: firstly saccharification is carried out to the grain finemeal, and then the protein residue after saccharification is separated and purified by the alkaline extraction and acid precipitation method for obtaining high-purity protein. The saccharification process solves the technical problems that some grain protein is combined with starch tightly and is not easy for being separated, and the extraction of protein is difficult; the alkaline extraction and acid precipitation process effectively improves the extraction rate and purity of grain protein; and the saccharification liquid can be used for other usages, thereby saving the resources to the largest extent. The protein extracted by the invention can be analyzed and determined with kjeldahl method-GB / T 5009.5 and is not less than 80 percent when being counted by crude protein content (NX6.25 dry basis), and the yield thereof is 6-10 percent.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry and relates to a protein separation and extraction process, in particular to a method for separating and extracting protein from grain fine powder. It is mainly suitable for rice, common barley, highland barley and other grains whose protein is not easy to extract. Background technique [0002] Protein is an indispensable nutrient for the human body. Grains such as rice and barley are rich in protein. At present, the methods for extracting protein from grains mainly include alkaline method, enzymatic method, alkaline enzyme two-step method, membrane separation and other extraction methods. However, due to the large number of disulfide bonds in rice protein, the protein structure is compact; and highland barley powder contains a large amount of amylopectin, which exists in a bonded state with highland barley protein, and there are a large number of disulfide bonds between highland ba...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14
Inventor 雷菊芳韩静
Owner 甘肃奇正实业(集团)有限公司
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