33 results about "Grain Proteins" patented technology

The invention discloses a cross-platform soybean seed protein content estimation method based on a spectral time sequence. The method comprises the following steps: step 1, acquiring high spectral data of a soybean canopy; 2, collecting a soybean multispectral image; step 3, acquiring soybean physiological parameters and grain protein content data; step 4, obtaining soybean photosynthetic parameter data; step 5, calculating a vegetation index for estimation; step 6, constructing a vegetation index time sequence; 7, screening an optimal time window; 8, extracting dynamic characteristics; step 9, establishing a model, estimating the soybean protein content, and performing precision verification; and step 10, carrying out mobility analysis on the model from a near-ground canopy to a satellite platform. According to the method, a theoretical basis and a practical path are provided for realizing remote sensing-based soybean GPC accurate monitoring, wide application of the method on a satellite remote sensing platform can be promoted in the future, and high-precision, high-time-efficiency and large-scale soybean GPC estimation is realized.

The invention discloses a primer combination for water saving and quality screening of winter wheat and application thereof, and relates to the technical field of molecular biology, the primer combination for water saving and quality screening of winter wheat comprises a primer pair P1 and a primer pair P2; according to the primer combination, the water-saving and drought-resistant characters are screened through PCR amplification, and SDS-PAGE electrophoresis is combined to detect grain protein subunit composition so as to screen quality characters. The efficient breeding method suitable for winter wheat is formed by designing a specific primer combination, constructing a molecular marker screening system and innovatively combining a cultivation management mode of water before drought and drought after water before winter wheat. According to the method, dependence on a single known gene is avoided, oriented improvement of water-saving drought resistance and quality is realized through collaborative screening and environment adaptability cultivation of a primer combination, and a new technical path is provided for wheat breeding in the arid region of North China.

Provided are a GPC1 gene and the use thereof. The GPC1 gene encodes an amino acid sequence as shown in SEQ ID NO. 2. An increase in the single-gene expression level of the GPC1 gene leads to an increase in the whole-grain protein content in rice, and knockout of the gene results in a decrease in the whole-grain protein content in rice.

A method and system for increasing the purity of protein from a system that produces protein, such as a high protein meal, from dry milling of grains including, for example, corn and wheat. The purity of the high protein meal, for example, can be increased by back end enzyme treatment of yeast, which has been separated along with protein from whole stillage. The enzyme(s) can breakdown the yeast into its yeast components that can be removed from the protein to provide for an increased purity of a resulting high protein product.

The present application relates to the technical field of animal feed, more particularly, to a methanol-based single-cell protein feed composition and a preparation method thereof.The feed composition comprises the following raw material components by weight: 30-70 parts of methanol-based single-cell protein powder, 10-40 parts of plant protein source, 10-30 parts of energy source, 1-10 parts of functional additive, and 5-20 parts of carrier.The present application uses the methanol-based single-cell protein, a non-grain protein source with high protein and good amino acid composition, to significantly improve the palatability, digestibility and nutritional value of the feed by compounding the methanol-based single-cell protein with conventional feed components through a scientific formula and a physical wall-breaking and mixing process, and to partially or completely replace traditional protein sources such as soybean meal, thereby reducing the cost of feed, alleviating the problem of food competition between humans and animals, and providing a sustainable new protein feed solution for the animal husbandry.

The present application relates to the technical field of plant genetic breeding, and particularly relates to a wheat grain protein content related protein, a coding gene thereof and application.The wheat grain protein content related protein is a TaNPF5.5-2A protein and / or a TaNPF5.5-2B protein.The research shows that the grain protein content and the total nitrogen content of the TaNPF5.5-2B overexpression transgenic wheat strain have a significant advantage.The present application screens and mines a new wheat grain protein content related gene, which not only provides a theoretical clue for the molecular genetic research of the wheat grain protein content, but also provides important gene resources for the molecular breeding of the wheat quality.

This invention discloses a fermentation control system and method based on multi-parameter collaborative control, belonging to the field of fermentation control technology. The system includes the following steps: collecting continuous data on temperature, humidity, airflow velocity, and heat flow distribution within the fermentation chamber and overlaying them with time markers; simultaneously recording the heating and ventilation response times to form a dynamic reference; tracking heat flow changes based on the time difference between heating and ventilation stages and extracting the convergence point, extending the heating cycle to form a time misalignment; adjusting the ventilation trigger interval in conjunction with the time misalignment buffer to couple and match air inflow with heat energy attenuation, constructing a stable heat exchange state; and implementing temporal perturbations based on this state, introducing delay differences to maintain asynchronous balance. This invention, through multi-parameter time correlation and temporal control, enables stable misalignment between temperature control and ventilation, suppressing heat flow resonance and stabilizing the temperature field; and maintains long-term asynchronous balance through temporal perturbations, ensuring stable microbial metabolism and improving the conversion efficiency of distiller's grains protein and the overall stability of the fermentation process.

The present application relates to a kind of spent grain protein peptide and its preparation method and application. Specifically, with beer brewing by-product spent grain as raw material, it is prepared by protease enzymolysis to prepare spent grain enzymolysis oligopeptide, and it is proved by mouse in-vivo experiment that the enzymolysis complex has the function of improving blood sugar, obesity, blood fat. On this basis, from the enzymolysis product, the key blood sugar reducing active peptide segment FPQPPQ, FPQQPPFG, FPQPQQPFP is identified, and it is verified that its alpha amylase inhibition rate is superior to the same concentration of acarbose. The spent grain protein peptide complex and spent grain protein peptide of the present application have the effect of reducing blood sugar, and can be applied to the development of food, health food, medicine and the like.

This invention discloses a primer combination for water-saving and quality screening of winter wheat and its application, relating to the field of molecular biology. The primer combination includes primer pair P1 and primer pair P2. This primer combination is used for molecular marker screening of water-saving and drought-resistant traits in winter wheat and can be combined with SDS-PAGE electrophoresis to detect grain protein subunit composition for screening quality traits in winter wheat. This invention designs specific primer combinations, constructs a molecular marker screening system, and innovatively combines it with the "pre-irrigation followed by drought" cultivation management model, forming a highly efficient breeding method suitable for winter wheat. This method avoids dependence on a single known gene, instead achieving targeted improvement of water-saving, drought-resistant, and quality through synergistic screening of primer combinations and environmental adaptability breeding, providing a new technical path for wheat breeding in the arid North China region.

The invention discloses an application of a soybean GmCIPK3 (GmCIPK3) protein coding gene GmCIPK3. The nucleotide sequence of the soybean GmCIPK3 protein coding gene GmCIPK3 is as shown in SEQ ID NO. 1. The constructed plant overexpression vector pBA002-GmCIPK3 is subjected to agrobacterium tumefaciens-mediated soybean genetic transformation to obtain a soybean receptor variety Tianlong, and it is found that compared with a receptor control group, the protein content in seeds of an overexpression transgenic soybean plant is increased, the oil content is reduced, and the protein quality of the seeds of the overexpression transgenic soybean plant is improved. Therefore, the gene can be introduced into plants as a target gene to improve the quality of soybean grain protein. Therefore, the new function of the soybean GmCIPK3 protein coding gene GmCIPK3 in soybean plants is found, the protein content and the oil content are changed through genetic engineering, and then the application in the aspect of transgenic soybean grain quality is affected.

This invention belongs to the field of molecular biology and genetic breeding technology, and discloses a QTL related to soybean seed protein content. qPro12 Tightly linked molecular markers and their applications. A genome-wide association study (GWAS) was used to identify a seed protein content QTL on soybean chromosome 12. qPro12 The significantly associated SNP is located at position 7,757,075 on chromosome 12 of the reference genome Glycine_max_v2.1, and can explain 0.51% of the phenotypic variation in the associated population. PARMS markers developed using this SNP were used to detect genotyping in 256 soybean varieties, demonstrating clear typing and ease of operation, making it suitable for molecular breeding of soybean grain protein content.

The invention belongs to the technical field of agriculture, and particularly relates to a molecular breeding method for improving wheat quality and application. According to the invention, key genetic regulation and control sites of kernel quality characters such as grain protein content, grain hardness and HMW-GS are integrated, a set of efficient wheat quality molecular marker selection system is constructed, and the invention aims to provide technical support for wheat quality precise breeding and assist efficient cultivation of high-quality wheat varieties.

The invention discloses a gene Dv-LMW10 for improving the processing quality of wheat bread and application of the gene Dv-LMW10. The CDS sequence of the haynaldia villosa Dv-LLW10 gene is as shown in SEQ ID NO: 1, the Dv-LLW10 genome sequence and the upstream 2000bp promoter sequence are as shown in SEQ ID NO: 2, and the coding amino acid sequence of the Dv-LLW10 gene is as shown in SEQ ID NO: 3. The Dv-LMW10 gene is converted into wheat under the driving of a promoter of the Dv-LMW10 gene and has the effect of obviously increasing the grain protein content, and the processing quality indexes such as the wet gluten content, the sedimentation value and the weakening degree are obviously superior to those of background and negative control materials. Therefore, the gene provided by the invention can be combined with a self promoter and then introduced into a proper expression vector and transformed into a plant host, so that the content of grain protein is changed, and the processing quality of wheat bread is improved.

The invention discloses an application of OsFTL1 and a coding gene thereof in improving protein content and chlorophyll content. The OsFTL1 disclosed by the invention is derived from rice and is a protein as shown in SEQ ID No. 1. Experiments prove that the OsFTL1 and the coding gene thereof can increase the chlorophyll content and the protein content, and under the normal nitrogen condition, the contents of chlorophyll a, chlorophyll b and carotenoid of leaves of the OsFTL1 transgenic plant are higher than those of wild plants; under the condition of low nitrogen, the leaf protein content of the OsFTL1 transgenic plant is obviously higher than that of a wild type; after the plants growing in the field are harvested, the grain protein content of the OsFTL1 transgenic plants is obviously higher than that of the wild plants. The results show that the OsFTL1 and the coding gene thereof can promote nitrogen assimilation, increase pigment synthesis and increase protein content, and have good application prospects.

The invention discloses a wheat grain protein content regulating gene TaPK-1B as well as an encoding protein and application thereof. The gene has a nucleotide sequence as shown in SEQ ID No.3, or a coding nucleotide sequence which is obtained by substitution, deletion or insertion of one or more nucleotides of the nucleotide sequence as shown in SEQ ID No.3 and has the same functional protein. The wheat TaPK-1B gene is excavated through whole genome association analysis, the wheat TaPK-1B gene is knocked out through genetic transformation, the wheat grain protein content can be increased, the function of the gene for negatively regulating the grain protein content is defined, a new gene resource is provided for wheat quality improvement, and an effective way is provided for wheat molecular breeding.

The invention discloses a preparation method of a cassava starch-waste syrup-vinasse protein composite formaldehyde-free binder. The environment-friendly formaldehyde-free binder is prepared by taking cassava starch, waste syrup and vinasse protein as main raw materials. The preparation method comprises the following steps: firstly, pretreating the vinasse protein extract, mixing with starch milk prepared in a certain proportion, gelatinizing, adding waste syrup, adjusting the pH value of a mixed solution, and then adding a cross-linking agent to realize a cross-linking reaction, thereby obtaining the binder. The binder prepared by the preparation method disclosed by the invention is free of formaldehyde emission in the production and use processes, and high-value utilization of various biomass resources such as waste syrup, vinasse and cassava starch is realized. The adhesive prepared by the invention has good adhesive property and water resistance, is green and environment-friendly, and can be widely applied to the field of artificial board manufacturing.

The invention discloses an application of a rice OsPIN1c protein and a related biological material thereof in regulating and controlling the grain length of rice seeds. In order to solve the problem of how to regulate and control the length of rice grains, the invention provides application of the protein or a substance for regulating and controlling the expression of a coding gene of the protein or a substance for regulating and controlling the activity or content of the protein in regulating and controlling the protein content of the plant grains, the name of the protein is OsPIN1c, and the amino acid sequence is shown as SEQ ID NO: 2. The invention provides a new germplasm resource for genetic breeding work, also provides a new direction of the rice OsPIN1c mutant plant in rice grain shape research, and has potential application value in the aspect of improving rice yield.

The utility model relates to the technical field of feed drying, and discloses an air flow drying device for vinasse protein feed production, which comprises a heating furnace, a pipeline is arranged on one side of the heating furnace, a driving piece is arranged below the pipeline, a feeding hopper for feeding vinasse protein feed is arranged above the pipeline, and the driving piece is arranged below the feeding hopper. A stirring and cleaning assembly for stirring the vinasse protein feed is arranged in an inner cavity of the feeding hopper, and the driving piece is provided with a linkage piece in a transmission mode; through cooperation of the driving part, the linkage part and the stirring and cleaning assembly, the driving part drives the linkage part to rotate, then the linkage part drives the stirring and cleaning assembly to rotate, and a lower scraping rod and a side scraping rod synchronously scrape vinasse protein feed adhering to the bottom and the side wall of the feeding hopper; therefore, the vinasse protein feed is prevented from being attached to the feeding hopper, the feeding efficiency of the vinasse protein feed is improved, meanwhile, the workload of workers for manually cleaning the feeding hopper in the later period can be reduced, and the drying efficiency of the vinasse protein feed is improved.

The invention relates to the technical field of high-value utilization of beer brewing byproducts, and discloses a spent grain all-component utilization protease-cellulase compound enzyme preparation which is prepared from the following raw materials in percentage by mass: 50-60% of protease, 10-30% of cellulase, 10-30% of hemicellulase and 10-30% of sodium citrate. 25%-35% of cellulase; 10%-20% of hemicellulase; the prepared compound enzyme preparation is applied to a spent grain conversion process of three-stage synergetic enzymolysis, by designing a staged enzymolysis process of acid first and alkali second, all enzyme systems are activated in sequence under the optimal condition, cellulase and hemicellulase break fiber barriers preferentially in the acid stage, and the enzyme systems are activated in sequence under the optimal condition. The method has the advantages that the method is simple and convenient to operate, the accessibility of subsequent protease to substrates is improved, the protein extraction rate and the fiber conversion rate are increased, the spent grain protein and fiber synchronous efficient conversion and almost all-component resource utilization are realized, and the problems of incomplete resource conversion and easy generation of secondary solid waste in a traditional treatment mode are thoroughly solved.

The invention belongs to the technical field of plant genetic engineering. In particular to application of the corn gene ZmARF16 in controlling the ear row number, the tassel branch number and the grain protein content. The protein coded by the gene is shown as SEQ ID NO.2. The invention also relates to application of the corn gene ZmARF16 in controlling the ear row number, tassel branch number and grain protein content. The gene is located in the fourth chromosome of corn and is used for controlling the corn tassel branch number, the corn ear row number and the grain protein content. The gene is knocked out by utilizing a CRISPR / Cas9 technology, gene expression is inhibited, the ear row number and the grain protein content of a corn inbred line in a normal environment can be increased, and the branch number of tassels is reduced.

The application discloses a kind of high-efficiency sterilization device for chicken manure conversion non-grain protein, including degradation component and steam generating component, degradation component includes reaction tank and stirring part, stirring part is located in reaction tank, stirring part includes driving shaft and stirring vane, reaction tank surface is provided with steam chamber, driving shaft is equipped with steam cavity and is penetrated with steam chamber, stirring vane is equipped with air groove, air groove is hourglass-shaped, the lower surface of stirring vane is fixedly connected with several evenly arranged nozzles, the output direction of nozzle is obliquely downward, nozzle, air groove and steam cavity are penetrated, steam generating component includes: superheated steam generator, pulse valve and pipe one, superheated steam generator is connected with pulse valve by pipe one, the output end of pulse valve is communicated with the inside of reaction tank, pipe one is communicated with steam chamber;Overall chicken manure sterilization is uniform and thorough, without new compound addition, treated material is pure, protein is not deteriorated during processing.

The invention discloses a goat non-grain protein compound functional feed, belongs to the technical field of animal feeds, and aims to solve the problems that the traditional goat daily ration depends on high-price protein raw materials such as soybean meal, the cost is high, anti-nutritional factors exist, agricultural by-products such as macadamia nut green peel are not effectively utilized, and the feed utilization rate is low. The technical problems of complex fermentation of single strain or simple strain combination, low raw material efficiency, incomplete degradation of antinutritional factors, easy contamination by infectious microbes and the like are solved. The feed comprises a beer residue mixed fermentation material, a needle mushroom mycelium high-protein raw material, a jasmine flower residue mixed fermentation material and other raw materials in specific parts by weight, beer residues and oat hulls are fermented according to the ratio, the needle mushroom mycelium high-protein raw material is prepared by inoculating needle mushroom strains with a specific culture medium, and jasmine flower residues and oat hulls are fermented according to the ratio. The feed also contains a premix and a mineral nutrient additive, and efficient utilization of the raw materials is realized through multi-strain synergistic fermentation and fungal conversion; the feed is mainly used for goat breeding, and can reduce feed cost and improve goat growth performance and feed utilization efficiency.

The invention discloses a method for extracting spent grain protein, the spent grain protein, a fruit and vegetable fresh-keeping agent and application. The method for extracting the spent grain protein comprises the following steps: uniformly mixing degreased spent grains with water, adjusting the pH value to be alkaline, carrying out alkaline extraction, and collecting an extracting solution; and carrying out acid precipitation on the extracting solution, collecting the precipitate, desalting, and preparing the spent grain protein. According to the method for extracting the spent grain protein, the yield, the purity and the functional characteristics of the spent grain protein are remarkably improved through the technologies of alkaline extraction and the like. The edible fruit and vegetable fresh-keeping agent prepared from the extracted spent grain protein can form a film on the surfaces of fruits and vegetables, has good moisture barrier property and antibacterial property, and can effectively prolong the normal-temperature storage period of the fruits and vegetables and enhance the storage stability of the fruits and vegetables.

The present application belongs to the technical field of natural polymer materials and cosmetic dressings, and particularly relates to a composite hydrogel facial mask dressing using spent grain as raw material and a preparation method thereof. The method comprises the following steps: dispersing spent grain protein obtained by extracting spent grain in water, and performing fiberization treatment under heating conditions to prepare spent grain protein fiber suspension; using solid residues after extracting spent grain protein from dry spent grain as raw material, obtaining purified cellulose after purification, and performing acidolysis reaction on the purified cellulose to prepare nanocrystalline cellulose suspension; mixing the spent grain protein fiber suspension and the nanocrystalline cellulose suspension, and inducing physical crosslinking under heating conditions to prepare spent grain protein fiber-nanocrystalline cellulose composite hydrogel. The composite hydrogel has significant shear thinning characteristics and relatively optimal viscosity, and has relatively high water retention rate and antioxidant activity.

The invention belongs to the field of gene engineering, and particularly relates to a gene and a method for improving corn characters. According to the invention, molecular markers are continuously developed in a QTL interval in several years, recombinant single plants are screened from offspring for offspring test, characters of tens of thousands of recombinant single plants are measured to reduce a functional interval, and finally, the functional gene ZCN9 capable of influencing the content of tocopherol in corn is finely positioned; the ZCN9 gene and the homologous gene ZCN10 of the ZCN9 gene are subjected to gene knockout so as to perform more detailed functional identification, and corn plants after the ZCN9 and ZCN10 genes are knocked out can increase the content of tocopherol in grains and improve the characters such as the flowering period, the plant height, the ear height, the grain protein content and the grain yield.

The invention discloses a primer group for detecting a KASP molecular marker related to the grain protein content and application of the primer group, and belongs to the technical field of biology. The wheat-aegilops tauschii introgression line and the secondary segregation population are utilized to finely locate a new major QTL existing in the aegilops tauschii parent and the aegilops tauschii introgression line, and the synergistic gene comes from aegilops tauschii, is not widely utilized by breeders, and has great potential application value. The invention also further develops a KASP molecular marker CS4D-19405835 which is closely linked with the content of the grain protein. High-quality germplasm resources, functional genes and molecular markers are provided for excavation and application of excellent-quality genes of aegilops tauschii, and wheat quality improvement is assisted.

The invention discloses an application of a wheat TaNAC025 gene in improving wheat grain protein, and belongs to the field of genetic engineering, the vector takes pBUE414 as a skeleton vector, and further comprises an sgRNA expression cassette and a Cas9 expression cassette, the Cas9 expression cassette is an expression cassette for regulating Cas9 expression by a Ubi promoter, and the sgRNA expression cassette sequentially comprises the following elements from upstream to downstream: a TaU3 promoter, sgRNA1 of TaNAC025 and a terminator OsU3t. By using the TaNAC025 gene knockout vector provided by the invention, TaNAC025 in wheat can be quickly knocked out, and the grain protein content of an obtained wheat material is remarkably increased.

The invention discloses an adversity regulation and control method for high-protein soybeans (the grain protein content is greater than or equal to 42%) encountering high-temperature stress (the daily maximum temperature is greater than or equal to 35 DEG C and lasts for more than 3 days) in the flowering period. According to the method, through the synergistic effect of physical cooling and soil moisture conservation, chemical stress resistance and flower protection and precise element regulation and control, flower falling and pod falling caused by high temperature are reduced, the yield is guaranteed, and meanwhile the high protein characteristic (the grain protein content is larger than or equal to 42%) is maintained. The method is suitable for high-protein soybean main producing areas such as south northeast, Huanghuai of North China and Yangtze River basin and summer high-temperature high-incidence areas.

Provided is the use of a TaMADS1-D1 gene in the regulation of wheat quality. TaMADS1-D1 can regulate the grain protein content and wet gluten content of wheat. Overexpression of the TaMADS1-D1 gene in wheat can significantly increase the grain protein content and wet gluten content of wheat.

The invention belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker closely linked with soybean grain protein content QTL qPro14 and application. A grain protein content QTL qPro14 is identified on the 14th chromosome of the soybean by utilizing whole genome association analysis, the remarkably associated SNP of the QTL qPro14 is located at the 1st, 360th and 542nd basic groups of the 14th chromosome of a reference genome Glycinemaxv2.1, and 0.32% of phenotypic variation can be explained. The PARMS marker developed by the SNP is used for detecting 256 soybean varieties, typing is clear, operation is easy and convenient, and the PARMS marker is suitable for soybean grain protein content molecular breeding.