41 results about "Grain Proteins" patented technology

The invention discloses an application of a soybean ZF-HD protein coding gene GmZFHD11 in the aspect of influencing the quality of soybean seeds. The nucleotide sequence of the soybean ZF-HD protein coding gene GmZFHD11 is as shown in SEQ ID NO. 1. The invention also discloses a preparation method of the soybean ZF-HD protein coding The constructed plant overexpression vector pBA002-GmZFHD11 is subjected to agrobacterium tumefaciens-mediated soybean genetic transformation to obtain a soybean receptor variety Jack, and it is found that compared with a receptor control group, the content of sulfur-containing amino acid in seeds is increased, the content of protein is increased, the content of beta-conglycinin (7S) is reduced, the content of glycinin (11S) is increased, and the 11S / 7S ratio is increased; and the quality of the overexpressed transgenic soybean strain seed protein is improved. Therefore, the gene can be introduced into plants as a target gene to improve the quality of soybean grain protein.

The invention discloses a cross-platform soybean seed protein content estimation method based on a spectral time sequence. The method comprises the following steps: step 1, acquiring high spectral data of a soybean canopy; 2, collecting a soybean multispectral image; step 3, acquiring soybean physiological parameters and grain protein content data; step 4, obtaining soybean photosynthetic parameter data; step 5, calculating a vegetation index for estimation; step 6, constructing a vegetation index time sequence; 7, screening an optimal time window; 8, extracting dynamic characteristics; step 9, establishing a model, estimating the soybean protein content, and performing precision verification; and step 10, carrying out mobility analysis on the model from a near-ground canopy to a satellite platform. According to the method, a theoretical basis and a practical path are provided for realizing remote sensing-based soybean GPC accurate monitoring, wide application of the method on a satellite remote sensing platform can be promoted in the future, and high-precision, high-time-efficiency and large-scale soybean GPC estimation is realized.

The invention discloses a primer combination for water saving and quality screening of winter wheat and application thereof, and relates to the technical field of molecular biology, the primer combination for water saving and quality screening of winter wheat comprises a primer pair P1 and a primer pair P2; according to the primer combination, the water-saving and drought-resistant characters are screened through PCR amplification, and SDS-PAGE electrophoresis is combined to detect grain protein subunit composition so as to screen quality characters. The efficient breeding method suitable for winter wheat is formed by designing a specific primer combination, constructing a molecular marker screening system and innovatively combining a cultivation management mode of water before drought and drought after water before winter wheat. According to the method, dependence on a single known gene is avoided, oriented improvement of water-saving drought resistance and quality is realized through collaborative screening and environment adaptability cultivation of a primer combination, and a new technical path is provided for wheat breeding in the arid region of North China.

Provided are a GPC1 gene and the use thereof. The GPC1 gene encodes an amino acid sequence as shown in SEQ ID NO. 2. An increase in the single-gene expression level of the GPC1 gene leads to an increase in the whole-grain protein content in rice, and knockout of the gene results in a decrease in the whole-grain protein content in rice.

A method and system for increasing the purity of protein from a system that produces protein, such as a high protein meal, from dry milling of grains including, for example, corn and wheat. The purity of the high protein meal, for example, can be increased by back end enzyme treatment of yeast, which has been separated along with protein from whole stillage. The enzyme(s) can breakdown the yeast into its yeast components that can be removed from the protein to provide for an increased purity of a resulting high protein product.

The invention relates to the technical field of food preparation, and discloses a preparation method and application of composite edible mushroom protein powder, and the preparation method comprises the following steps: step 1, edible mushroom protein preparation and whey protein treatment; 2, adopting a three-stage gradient mixing method; 3, low-temperature pasteurization is combined with microwave sterilization; step 4, carrying out superfine grinding on the dried material, passing through a 300-mesh sieve, carrying out nitrogen-filled packaging, and sealing and storing in a shady, cool and dry place; a superfine grinding technology and a three-stage gradient mixing technology are adopted, so that protein powder particles are uniformly dispersed, the solubility is greatly improved, meanwhile, coarse particle protein powder is greatly improved, and the protein powder is particularly suitable for people with weak digestive functions such as old people and postoperative patients; according to the process, the heat-sensitive components are effectively protected while microbial indexes are ensured to reach the standard, and compared with traditional high-temperature sterilization, the process has the advantage that the activity retention rate of the ganoderma lucidum polysaccharide is effectively increased.

The present application relates to the technical field of animal feed, more particularly, to a methanol-based single-cell protein feed composition and a preparation method thereof.The feed composition comprises the following raw material components by weight: 30-70 parts of methanol-based single-cell protein powder, 10-40 parts of plant protein source, 10-30 parts of energy source, 1-10 parts of functional additive, and 5-20 parts of carrier.The present application uses the methanol-based single-cell protein, a non-grain protein source with high protein and good amino acid composition, to significantly improve the palatability, digestibility and nutritional value of the feed by compounding the methanol-based single-cell protein with conventional feed components through a scientific formula and a physical wall-breaking and mixing process, and to partially or completely replace traditional protein sources such as soybean meal, thereby reducing the cost of feed, alleviating the problem of food competition between humans and animals, and providing a sustainable new protein feed solution for the animal husbandry.

The present application relates to the technical field of plant genetic breeding, and particularly relates to a wheat grain protein content related protein, a coding gene thereof and application.The wheat grain protein content related protein is a TaNPF5.5-2A protein and / or a TaNPF5.5-2B protein.The research shows that the grain protein content and the total nitrogen content of the TaNPF5.5-2B overexpression transgenic wheat strain have a significant advantage.The present application screens and mines a new wheat grain protein content related gene, which not only provides a theoretical clue for the molecular genetic research of the wheat grain protein content, but also provides important gene resources for the molecular breeding of the wheat quality.

This invention discloses a fermentation control system and method based on multi-parameter collaborative control, belonging to the field of fermentation control technology. The system includes the following steps: collecting continuous data on temperature, humidity, airflow velocity, and heat flow distribution within the fermentation chamber and overlaying them with time markers; simultaneously recording the heating and ventilation response times to form a dynamic reference; tracking heat flow changes based on the time difference between heating and ventilation stages and extracting the convergence point, extending the heating cycle to form a time misalignment; adjusting the ventilation trigger interval in conjunction with the time misalignment buffer to couple and match air inflow with heat energy attenuation, constructing a stable heat exchange state; and implementing temporal perturbations based on this state, introducing delay differences to maintain asynchronous balance. This invention, through multi-parameter time correlation and temporal control, enables stable misalignment between temperature control and ventilation, suppressing heat flow resonance and stabilizing the temperature field; and maintains long-term asynchronous balance through temporal perturbations, ensuring stable microbial metabolism and improving the conversion efficiency of distiller's grains protein and the overall stability of the fermentation process.

The present application relates to a kind of spent grain protein peptide and its preparation method and application. Specifically, with beer brewing by-product spent grain as raw material, it is prepared by protease enzymolysis to prepare spent grain enzymolysis oligopeptide, and it is proved by mouse in-vivo experiment that the enzymolysis complex has the function of improving blood sugar, obesity, blood fat. On this basis, from the enzymolysis product, the key blood sugar reducing active peptide segment FPQPPQ, FPQQPPFG, FPQPQQPFP is identified, and it is verified that its alpha amylase inhibition rate is superior to the same concentration of acarbose. The spent grain protein peptide complex and spent grain protein peptide of the present application have the effect of reducing blood sugar, and can be applied to the development of food, health food, medicine and the like.

This invention discloses a primer combination for water-saving and quality screening of winter wheat and its application, relating to the field of molecular biology. The primer combination includes primer pair P1 and primer pair P2. This primer combination is used for molecular marker screening of water-saving and drought-resistant traits in winter wheat and can be combined with SDS-PAGE electrophoresis to detect grain protein subunit composition for screening quality traits in winter wheat. This invention designs specific primer combinations, constructs a molecular marker screening system, and innovatively combines it with the "pre-irrigation followed by drought" cultivation management model, forming a highly efficient breeding method suitable for winter wheat. This method avoids dependence on a single known gene, instead achieving targeted improvement of water-saving, drought-resistant, and quality through synergistic screening of primer combinations and environmental adaptability breeding, providing a new technical path for wheat breeding in the arid North China region.

The invention discloses a preparation method and application of peony seed protein with high surface hydrophobicity. The method comprises the following steps: washing and drying peony seeds, carrying out superfine grinding treatment, removing grease through a squeezing method, mixing peony seed meal with distilled water according to a certain proportion, carrying out homogenization pretreatment, adopting an ultrasonic physical field superposition auxiliary alkali extraction method, and carrying out operations of multiple times of acid precipitation, redissolution, centrifugation, dialysis and the like, so as to obtain the peony seed meal. And finally freeze-drying to obtain powdery peony seed protein. The peony seed protein obtained by the method has high surface hydrophobicity, the foam property of the protein is remarkably improved, and the protein yield is also remarkably increased. A new technical route is provided for extraction and preparation of protein with high surface hydrophobicity in plant source grain protein, high-quality novel plant protein resources are provided for industries such as food, medicine and cosmetics, and the method has wide application prospects and market potential.

The invention discloses an application of a soybean GmCIPK3 (GmCIPK3) protein coding gene GmCIPK3. The nucleotide sequence of the soybean GmCIPK3 protein coding gene GmCIPK3 is as shown in SEQ ID NO. 1. The constructed plant overexpression vector pBA002-GmCIPK3 is subjected to agrobacterium tumefaciens-mediated soybean genetic transformation to obtain a soybean receptor variety Tianlong, and it is found that compared with a receptor control group, the protein content in seeds of an overexpression transgenic soybean plant is increased, the oil content is reduced, and the protein quality of the seeds of the overexpression transgenic soybean plant is improved. Therefore, the gene can be introduced into plants as a target gene to improve the quality of soybean grain protein. Therefore, the new function of the soybean GmCIPK3 protein coding gene GmCIPK3 in soybean plants is found, the protein content and the oil content are changed through genetic engineering, and then the application in the aspect of transgenic soybean grain quality is affected.

This invention belongs to the field of molecular biology and genetic breeding technology, and discloses a QTL related to soybean seed protein content. qPro12 Tightly linked molecular markers and their applications. A genome-wide association study (GWAS) was used to identify a seed protein content QTL on soybean chromosome 12. qPro12 The significantly associated SNP is located at position 7,757,075 on chromosome 12 of the reference genome Glycine_max_v2.1, and can explain 0.51% of the phenotypic variation in the associated population. PARMS markers developed using this SNP were used to detect genotyping in 256 soybean varieties, demonstrating clear typing and ease of operation, making it suitable for molecular breeding of soybean grain protein content.

The invention belongs to the technical field of agriculture, and particularly relates to a molecular breeding method for improving wheat quality and application. According to the invention, key genetic regulation and control sites of kernel quality characters such as grain protein content, grain hardness and HMW-GS are integrated, a set of efficient wheat quality molecular marker selection system is constructed, and the invention aims to provide technical support for wheat quality precise breeding and assist efficient cultivation of high-quality wheat varieties.

The invention discloses a gene Dv-LMW10 for improving the processing quality of wheat bread and application of the gene Dv-LMW10. The CDS sequence of the haynaldia villosa Dv-LLW10 gene is as shown in SEQ ID NO: 1, the Dv-LLW10 genome sequence and the upstream 2000bp promoter sequence are as shown in SEQ ID NO: 2, and the coding amino acid sequence of the Dv-LLW10 gene is as shown in SEQ ID NO: 3. The Dv-LMW10 gene is converted into wheat under the driving of a promoter of the Dv-LMW10 gene and has the effect of obviously increasing the grain protein content, and the processing quality indexes such as the wet gluten content, the sedimentation value and the weakening degree are obviously superior to those of background and negative control materials. Therefore, the gene provided by the invention can be combined with a self promoter and then introduced into a proper expression vector and transformed into a plant host, so that the content of grain protein is changed, and the processing quality of wheat bread is improved.

The invention discloses an application of OsFTL1 and a coding gene thereof in improving protein content and chlorophyll content. The OsFTL1 disclosed by the invention is derived from rice and is a protein as shown in SEQ ID No. 1. Experiments prove that the OsFTL1 and the coding gene thereof can increase the chlorophyll content and the protein content, and under the normal nitrogen condition, the contents of chlorophyll a, chlorophyll b and carotenoid of leaves of the OsFTL1 transgenic plant are higher than those of wild plants; under the condition of low nitrogen, the leaf protein content of the OsFTL1 transgenic plant is obviously higher than that of a wild type; after the plants growing in the field are harvested, the grain protein content of the OsFTL1 transgenic plants is obviously higher than that of the wild plants. The results show that the OsFTL1 and the coding gene thereof can promote nitrogen assimilation, increase pigment synthesis and increase protein content, and have good application prospects.

The present invention discloses a device for extracting and separating protein from grains. The present invention relates to the technical field of protein extraction and separation, and comprises a wrapping shell, wherein the outer surface of the wrapping shell is fixedly connected to a connecting plate, and the connecting plate is fixedly connected to a frame on a side away from the wrapping shell; the upper surface of the frame is provided with a shaking mechanism, and by providing the shaking mechanism, the grains that need protein extraction can be shaken, and rice husks or impurities in the grains can be removed during the shaking process, thereby preventing rice husks from being mixed in when the grains are ground, thereby achieving the effect of increasing the protein extraction rate; the shaking mechanism comprises a first fixed frame, the first fixed frame passes through the outer side surface of the wrapping shell, the inner cavity of the first fixed frame is fixedly connected to a track rod, and the outer surface of the track rod is slidably connected to a sliding frame, thereby achieving the effect of removing rice husks and residues in the grains and extracting protein.

The invention discloses a wheat grain protein content regulating gene TaPK-1B as well as an encoding protein and application thereof. The gene has a nucleotide sequence as shown in SEQ ID No.3, or a coding nucleotide sequence which is obtained by substitution, deletion or insertion of one or more nucleotides of the nucleotide sequence as shown in SEQ ID No.3 and has the same functional protein. The wheat TaPK-1B gene is excavated through whole genome association analysis, the wheat TaPK-1B gene is knocked out through genetic transformation, the wheat grain protein content can be increased, the function of the gene for negatively regulating the grain protein content is defined, a new gene resource is provided for wheat quality improvement, and an effective way is provided for wheat molecular breeding.

A provincial-scale prediction method of a winter wheat grain protein content includes obtaining crop canopy spectral information of different spatial resolutions and spectral resolutions during a wheat growth period, performing preprocessing on the spectral information, performing feature selection based on pixel-wise phenology matching method, calculating, pixel by pixel, a maximal value of a spectral vegetation index within 90 days of each pixel maturity period, and selecting a vegetation index most highly correlated with the grain protein content for correlation analysis, and constructing a hierarchical linear model to perform prediction on the winter wheat grain protein content. In the present disclosure, the phenological difference of the relationship of remote-sensing information, climatic information and grain protein content is taken into account comprehensively, and pixel-wise phenology matching and hierarchical linear model are introduced, such that with key environmental factors as difference factors of the regional grain protein model.

The invention discloses a preparation method of a cassava starch-waste syrup-vinasse protein composite formaldehyde-free binder. The environment-friendly formaldehyde-free binder is prepared by taking cassava starch, waste syrup and vinasse protein as main raw materials. The preparation method comprises the following steps: firstly, pretreating the vinasse protein extract, mixing with starch milk prepared in a certain proportion, gelatinizing, adding waste syrup, adjusting the pH value of a mixed solution, and then adding a cross-linking agent to realize a cross-linking reaction, thereby obtaining the binder. The binder prepared by the preparation method disclosed by the invention is free of formaldehyde emission in the production and use processes, and high-value utilization of various biomass resources such as waste syrup, vinasse and cassava starch is realized. The adhesive prepared by the invention has good adhesive property and water resistance, is green and environment-friendly, and can be widely applied to the field of artificial board manufacturing.

The invention discloses an application of a rice OsPIN1c protein and a related biological material thereof in regulating and controlling the grain length of rice seeds. In order to solve the problem of how to regulate and control the length of rice grains, the invention provides application of the protein or a substance for regulating and controlling the expression of a coding gene of the protein or a substance for regulating and controlling the activity or content of the protein in regulating and controlling the protein content of the plant grains, the name of the protein is OsPIN1c, and the amino acid sequence is shown as SEQ ID NO: 2. The invention provides a new germplasm resource for genetic breeding work, also provides a new direction of the rice OsPIN1c mutant plant in rice grain shape research, and has potential application value in the aspect of improving rice yield.

The utility model relates to the technical field of feed drying, and discloses an air flow drying device for vinasse protein feed production, which comprises a heating furnace, a pipeline is arranged on one side of the heating furnace, a driving piece is arranged below the pipeline, a feeding hopper for feeding vinasse protein feed is arranged above the pipeline, and the driving piece is arranged below the feeding hopper. A stirring and cleaning assembly for stirring the vinasse protein feed is arranged in an inner cavity of the feeding hopper, and the driving piece is provided with a linkage piece in a transmission mode; through cooperation of the driving part, the linkage part and the stirring and cleaning assembly, the driving part drives the linkage part to rotate, then the linkage part drives the stirring and cleaning assembly to rotate, and a lower scraping rod and a side scraping rod synchronously scrape vinasse protein feed adhering to the bottom and the side wall of the feeding hopper; therefore, the vinasse protein feed is prevented from being attached to the feeding hopper, the feeding efficiency of the vinasse protein feed is improved, meanwhile, the workload of workers for manually cleaning the feeding hopper in the later period can be reduced, and the drying efficiency of the vinasse protein feed is improved.

The invention relates to the technical field of high-value utilization of beer brewing byproducts, and discloses a spent grain all-component utilization protease-cellulase compound enzyme preparation which is prepared from the following raw materials in percentage by mass: 50-60% of protease, 10-30% of cellulase, 10-30% of hemicellulase and 10-30% of sodium citrate. 25%-35% of cellulase; 10%-20% of hemicellulase; the prepared compound enzyme preparation is applied to a spent grain conversion process of three-stage synergetic enzymolysis, by designing a staged enzymolysis process of acid first and alkali second, all enzyme systems are activated in sequence under the optimal condition, cellulase and hemicellulase break fiber barriers preferentially in the acid stage, and the enzyme systems are activated in sequence under the optimal condition. The method has the advantages that the method is simple and convenient to operate, the accessibility of subsequent protease to substrates is improved, the protein extraction rate and the fiber conversion rate are increased, the spent grain protein and fiber synchronous efficient conversion and almost all-component resource utilization are realized, and the problems of incomplete resource conversion and easy generation of secondary solid waste in a traditional treatment mode are thoroughly solved.

Described are non-animal protein blends comprising legume protein, and one or more of synthetic protein, seed protein, and grain protein, wherein the blend has a PDCAAS of 1. Compositions comprising these blends and methods of using them are also disclosed.

The invention belongs to the technical field of plant genetic engineering. In particular to application of the corn gene ZmARF16 in controlling the ear row number, the tassel branch number and the grain protein content. The protein coded by the gene is shown as SEQ ID NO.2. The invention also relates to application of the corn gene ZmARF16 in controlling the ear row number, tassel branch number and grain protein content. The gene is located in the fourth chromosome of corn and is used for controlling the corn tassel branch number, the corn ear row number and the grain protein content. The gene is knocked out by utilizing a CRISPR / Cas9 technology, gene expression is inhibited, the ear row number and the grain protein content of a corn inbred line in a normal environment can be increased, and the branch number of tassels is reduced.

The application discloses a kind of high-efficiency sterilization device for chicken manure conversion non-grain protein, including degradation component and steam generating component, degradation component includes reaction tank and stirring part, stirring part is located in reaction tank, stirring part includes driving shaft and stirring vane, reaction tank surface is provided with steam chamber, driving shaft is equipped with steam cavity and is penetrated with steam chamber, stirring vane is equipped with air groove, air groove is hourglass-shaped, the lower surface of stirring vane is fixedly connected with several evenly arranged nozzles, the output direction of nozzle is obliquely downward, nozzle, air groove and steam cavity are penetrated, steam generating component includes: superheated steam generator, pulse valve and pipe one, superheated steam generator is connected with pulse valve by pipe one, the output end of pulse valve is communicated with the inside of reaction tank, pipe one is communicated with steam chamber;Overall chicken manure sterilization is uniform and thorough, without new compound addition, treated material is pure, protein is not deteriorated during processing.

The invention relates to the field of biomass resource fermentation and refining, in particular to a method for pretreating vinasse and fermenting vinasse protein. The method comprises the following steps: uniformly mixing vinasse with a lignocellulose raw material, controlling the water content to be 20-40wt%, granulating by using a flat die granulator, filling into a closed container, and hermetically storing or intermittently ventilating during hermetically storing the vinasse by using heteroacid and other substances of the vinasse under the conditions of normal temperature and normal pressure, so as to prepare pretreated vinasse; furthermore, the pretreatment efficiency is improved in manners of adding a surfactant, spraying a compound enzyme preparation and the like. The vinasse pretreatment method disclosed by the invention is simple and convenient to operate, high in treatment capacity, low in sugar loss, low in cost and easy for large-scale production, and a lignocellulose structure of the vinasse can be effectively destroyed; meanwhile, intake of acid and alkali is reduced, toxic degradation products are few, and treatment conditions are mild and environmentally friendly. By utilizing the pretreated vinasse to ferment vinasse protein and regulating and controlling fermentation conditions, the cost can be remarkably reduced, the fermentation period is shortened, the vinasse protein content is increased, and the method is suitable for industrial mass production.

The invention discloses a goat non-grain protein compound functional feed, belongs to the technical field of animal feeds, and aims to solve the problems that the traditional goat daily ration depends on high-price protein raw materials such as soybean meal, the cost is high, anti-nutritional factors exist, agricultural by-products such as macadamia nut green peel are not effectively utilized, and the feed utilization rate is low. The technical problems of complex fermentation of single strain or simple strain combination, low raw material efficiency, incomplete degradation of antinutritional factors, easy contamination by infectious microbes and the like are solved. The feed comprises a beer residue mixed fermentation material, a needle mushroom mycelium high-protein raw material, a jasmine flower residue mixed fermentation material and other raw materials in specific parts by weight, beer residues and oat hulls are fermented according to the ratio, the needle mushroom mycelium high-protein raw material is prepared by inoculating needle mushroom strains with a specific culture medium, and jasmine flower residues and oat hulls are fermented according to the ratio. The feed also contains a premix and a mineral nutrient additive, and efficient utilization of the raw materials is realized through multi-strain synergistic fermentation and fungal conversion; the feed is mainly used for goat breeding, and can reduce feed cost and improve goat growth performance and feed utilization efficiency.

The invention discloses a method for extracting spent grain protein, the spent grain protein, a fruit and vegetable fresh-keeping agent and application. The method for extracting the spent grain protein comprises the following steps: uniformly mixing degreased spent grains with water, adjusting the pH value to be alkaline, carrying out alkaline extraction, and collecting an extracting solution; and carrying out acid precipitation on the extracting solution, collecting the precipitate, desalting, and preparing the spent grain protein. According to the method for extracting the spent grain protein, the yield, the purity and the functional characteristics of the spent grain protein are remarkably improved through the technologies of alkaline extraction and the like. The edible fruit and vegetable fresh-keeping agent prepared from the extracted spent grain protein can form a film on the surfaces of fruits and vegetables, has good moisture barrier property and antibacterial property, and can effectively prolong the normal-temperature storage period of the fruits and vegetables and enhance the storage stability of the fruits and vegetables.