66 results about "Grain Proteins" patented technology

Provided is the use of a protein derived from wheat and a biomaterial related thereto in increasing the yield per plant and grain protein content of a crop. The protein is designated as SWEET11, and may be a protein having an amino acid sequence as shown in sequence 9, sequence 6 or sequence 3 in the sequence listing. The wheat SWEET11 protein (TaSWEET11) is over-expressed in the Zhengmai 7698 wheat variety (nationally approved), and it is found that the over-expression of TaSWEET11 can significantly improve the thousand-grain weight of wheat grains and increase the yield. In addition, the over-expression of TaSWEET11 also increases the protein content in the wheat grain, significantly improving the grain quality while increasing the yield. The TaSWEET11 protein can be used for breeding and quality improvement of crops.

The invention discloses an application of a soybean ZF-HD protein coding gene GmZFHD11 in the aspect of influencing the quality of soybean seeds. The nucleotide sequence of the soybean ZF-HD protein coding gene GmZFHD11 is as shown in SEQ ID NO. 1. The invention also discloses a preparation method of the soybean ZF-HD protein coding The constructed plant overexpression vector pBA002-GmZFHD11 is subjected to agrobacterium tumefaciens-mediated soybean genetic transformation to obtain a soybean receptor variety Jack, and it is found that compared with a receptor control group, the content of sulfur-containing amino acid in seeds is increased, the content of protein is increased, the content of beta-conglycinin (7S) is reduced, the content of glycinin (11S) is increased, and the 11S / 7S ratio is increased; and the quality of the overexpressed transgenic soybean strain seed protein is improved. Therefore, the gene can be introduced into plants as a target gene to improve the quality of soybean grain protein.

The invention discloses a cross-platform soybean seed protein content estimation method based on a spectral time sequence. The method comprises the following steps: step 1, acquiring high spectral data of a soybean canopy; 2, collecting a soybean multispectral image; step 3, acquiring soybean physiological parameters and grain protein content data; step 4, obtaining soybean photosynthetic parameter data; step 5, calculating a vegetation index for estimation; step 6, constructing a vegetation index time sequence; 7, screening an optimal time window; 8, extracting dynamic characteristics; step 9, establishing a model, estimating the soybean protein content, and performing precision verification; and step 10, carrying out mobility analysis on the model from a near-ground canopy to a satellite platform. According to the method, a theoretical basis and a practical path are provided for realizing remote sensing-based soybean GPC accurate monitoring, wide application of the method on a satellite remote sensing platform can be promoted in the future, and high-precision, high-time-efficiency and large-scale soybean GPC estimation is realized.

The invention belongs to the technical field of food, and particularly relates to a method for preparing coffee mate from brewer's grains. According to the method, the brewer's grains are crushed and then subjected to alkali cooking, then high-pressure homogenization treatment is combined, protein in the brewer's grains is released, the brewer's grain protein can be adsorbed on an oil-water interface, a stable emulsion is formed, and the purpose of preparing the coffee mate from the brewer's grains is achieved. Meanwhile, the brewer's grain contains various nutritional ingredients and health-care ingredients, and when the brewer's grain is used as a coffee mate, the nutritional value of coffee is increased, the flavor of the coffee is improved, and a certain health-care effect can be achieved. Results of the embodiment show that after the coffee mate prepared by the method provided by the invention is added into coffee, the coffee mate is closest to a commercially available Nestle coffee mate in chromaticity, appearance and particle size distribution, and the coffee mate has application potential.

The invention provides a novel compound feed for leuciscus waleckii, which comprises the following components: 10 to 30 percent of fish meal, 8 to 12 percent of soybean protein concentrate, 15 to 20 percent of flour, 3 to 6 percent of casein, 0.5 to 2 percent of gelatin, 4 to 6 percent of corn protein powder, 2 to 3 percent of soybean oil, 2 to 3 percent of fish oil, 18 to 24 percent of dextrin, 0.3 to 0.6 percent of mineral substance and vitamin premix, 0.2 to 0.6 percent of choline chloride, 1.5 to 2.5 percent of monocalcium phosphate, 0.05 to 0.12 percent of vitamin C ester, 2.5 to 3.5 percent of microcrystalline cellulose and the balance of water. The non-grain novel protein source defatted tenebrio molitor powder is used for partially replacing the fish meal, and the replacement proportion is 0-60%. The invention further provides a preparation method of the novel compound feed for the leuciscus waleckii, and the obtained feed is used for the leuciscus waleckii and can promote the growth performance, the digestive function, the protein metabolism function, the lipid metabolism function, the immune function and the oxidation resistance of the leuciscus waleckii. The invention provides a theoretical reference for developing a novel non-grain protein source, saving fish meal protein, reducing the feed cost and optimizing the artificial compound feed for the leuciscus waleckii.

The invention discloses rice LOC_Os01g68930 The application of genes in regulating the protein content in rice grains relates to the field of genetic engineering technology. LOC_Os01g68930 The CDS sequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence of the protein encoded by it is shown in SEQ ID NO. 2. The present invention obtains OE‑LOC_Os01g68930 Overexpression plants. Experiments have shown that: PLACE_ Os01g68930 The total content of glutenin and alcohol-soluble proteins in the seeds of overexpressing transgenic rice reached 7.88% to 7.97%. In comparison, the protein content of wild-type rice grains was 6.97%, and the protein content of transgenic rice grains increased by 13.1%-14.3%. LOC_Os01g68930 It has been confirmed that this gene can effectively regulate the protein content of rice grains, laying a solid foundation for the subsequent breeding of high-protein rice varieties.

The invention discloses a primer combination for water saving and quality screening of winter wheat and application thereof, and relates to the technical field of molecular biology, the primer combination for water saving and quality screening of winter wheat comprises a primer pair P1 and a primer pair P2; according to the primer combination, the water-saving and drought-resistant characters are screened through PCR amplification, and SDS-PAGE electrophoresis is combined to detect grain protein subunit composition so as to screen quality characters. The efficient breeding method suitable for winter wheat is formed by designing a specific primer combination, constructing a molecular marker screening system and innovatively combining a cultivation management mode of water before drought and drought after water before winter wheat. According to the method, dependence on a single known gene is avoided, oriented improvement of water-saving drought resistance and quality is realized through collaborative screening and environment adaptability cultivation of a primer combination, and a new technical path is provided for wheat breeding in the arid region of North China.

Provided are a GPC1 gene and the use thereof. The GPC1 gene encodes an amino acid sequence as shown in SEQ ID NO. 2. An increase in the single-gene expression level of the GPC1 gene leads to an increase in the whole-grain protein content in rice, and knockout of the gene results in a decrease in the whole-grain protein content in rice.

A method and system for increasing the purity of protein from a system that produces protein, such as a high protein meal, from dry milling of grains including, for example, corn and wheat. The purity of the high protein meal, for example, can be increased by back end enzyme treatment of yeast, which has been separated along with protein from whole stillage. The enzyme(s) can breakdown the yeast into its yeast components that can be removed from the protein to provide for an increased purity of a resulting high protein product.

The invention belongs to the technical field of biology, and particularly relates to a multifunctional bioactive peptide based on highland barley vinasse protein and a preparation method thereof, and the amino acid sequence of the bioactive peptide is shown as SEQ ID NO: 1. The bioactive peptide disclosed by the invention is small in molecular weight and easy to absorb, can inhibit tyrosinase activity and efficiently scavenge free radicals, and has the advantages of no toxicity, no carcinogenicity, high hydrophilicity, longer half-life period and the like through biosignal analysis and prediction; the compound can be applied to development of drugs for treating diseases related to skin pigmentation and oxidative stress or daily chemical products for whitening, anti-aging, anti-oxidation and the like, and has wide application prospects.

The invention provides a crop protein content remote sensing monitoring method based on atlas synergy, and relates to the technical field of crop quality monitoring, and the method comprises the steps: obtaining a remote sensing image of a target crop, calculating texture parameters, structure parameters, growth vigor parameters and pigment parameters of the target crop, and predicting the protein content of the target crop through a grain protein content prediction model. Obtaining the protein content of the target crop. The crop protein content remote sensing monitoring method based on map collaboration provided by the invention realizes efficient and accurate monitoring of the grain protein content of crops in the field scale, can provide a decision basis for crop cultivation management and agricultural production strategy adjustment, promotes improvement of crop quality, and serves precision agriculture.

The invention relates to the technical field of food preparation, and discloses a preparation method and application of composite edible mushroom protein powder, and the preparation method comprises the following steps: step 1, edible mushroom protein preparation and whey protein treatment; 2, adopting a three-stage gradient mixing method; 3, low-temperature pasteurization is combined with microwave sterilization; step 4, carrying out superfine grinding on the dried material, passing through a 300-mesh sieve, carrying out nitrogen-filled packaging, and sealing and storing in a shady, cool and dry place; a superfine grinding technology and a three-stage gradient mixing technology are adopted, so that protein powder particles are uniformly dispersed, the solubility is greatly improved, meanwhile, coarse particle protein powder is greatly improved, and the protein powder is particularly suitable for people with weak digestive functions such as old people and postoperative patients; according to the process, the heat-sensitive components are effectively protected while microbial indexes are ensured to reach the standard, and compared with traditional high-temperature sterilization, the process has the advantage that the activity retention rate of the ganoderma lucidum polysaccharide is effectively increased.

The invention provides a major QTL (Quantitative Trait Loci) for identifying corn kernel protein as well as a primer group, a kit and a method thereof, and belongs to the field of biology. The primer group comprises a forward primer, a first reverse primer and a second reverse primer, the sequence of the forward primer is shown as SEQ ID NO: 1 in a sequence table, the first reverse primer is shown as SEQ ID NO: 2 in the sequence table, and the second reverse primer is shown as SEQ ID NO: 3 in the sequence table. When the primer group, the kit and the method provided by the embodiment are used for detection, the result is consistent with the grain protein content measured by a near infrared spectrum analyzer, and meanwhile, the method provided by the embodiment is simple and convenient to operate and clear in typing, and can be used for molecular marker-assisted selective breeding of the corn grain protein content.

The present application relates to the technical field of animal feed, more particularly, to a methanol-based single-cell protein feed composition and a preparation method thereof.The feed composition comprises the following raw material components by weight: 30-70 parts of methanol-based single-cell protein powder, 10-40 parts of plant protein source, 10-30 parts of energy source, 1-10 parts of functional additive, and 5-20 parts of carrier.The present application uses the methanol-based single-cell protein, a non-grain protein source with high protein and good amino acid composition, to significantly improve the palatability, digestibility and nutritional value of the feed by compounding the methanol-based single-cell protein with conventional feed components through a scientific formula and a physical wall-breaking and mixing process, and to partially or completely replace traditional protein sources such as soybean meal, thereby reducing the cost of feed, alleviating the problem of food competition between humans and animals, and providing a sustainable new protein feed solution for the animal husbandry.

This tomato sauce contains 15-25 mass% of a tomato paste, 0-10 mass% of tomato ketchup, and 0.1-0.5 mass% of a grain protein hydrolysate, and has a total acidity of 0.8-2.0%. The grain protein hydrolysate is preferably an acid hydrolysate of a soybean protein. The mass ratio of acidic amino acid to basic amino acid (acidic amino acid : basic amino acid) is preferably 1.5-2.0 : 1.0 when the amount of amino acid contained in the grain protein hydrolysate is measured by an amino acid analyzer.

The invention discloses a siraitia australis feed as well as a preparation method and application thereof, and relates to the technical field of aquaculture. The feed comprises the following raw materials in parts by mass: 20-28 parts of fish meal, 14-22 parts of corn flour, 12-16 parts of clostridium ethanol protein, 10-14 parts of cottonseed protein concentrate, 8-12 parts of rice bran, 6-10 parts of tenebrio molitor powder, 2-6 parts of casein, 1-5 parts of chlorella powder, 1-3 parts of fish oil, 1-3 parts of soybean oil, 1-3 parts of a multi-vitamin multi-mineral premix and 0.5-1.5 parts of sodium carboxymethyl cellulose. Specific non-grain protein raw materials and easily available corn flour, rice bran and other raw materials are added into the feed to optimize the feed formula, the purposes of reducing cost and improving efficiency are achieved, and the developed special feed for the juvenile fishes of the onychostoma simachiae has the advantages that the special feed is suitable for the juvenile fishes of the onychostoma simachiae and the juvenile fishes of the onychostoma simachiae; the survival rate and the growth performance of the juvenile fishes of the onychostoma hirsutum are improved, and a solid foundation is laid for promoting resource protection, development and utilization of the onychostoma hirsutum.

The present application relates to the technical field of plant genetic breeding, and particularly relates to a wheat grain protein content related protein, a coding gene thereof and application.The wheat grain protein content related protein is a TaNPF5.5-2A protein and / or a TaNPF5.5-2B protein.The research shows that the grain protein content and the total nitrogen content of the TaNPF5.5-2B overexpression transgenic wheat strain have a significant advantage.The present application screens and mines a new wheat grain protein content related gene, which not only provides a theoretical clue for the molecular genetic research of the wheat grain protein content, but also provides important gene resources for the molecular breeding of the wheat quality.

This invention discloses a fermentation control system and method based on multi-parameter collaborative control, belonging to the field of fermentation control technology. The system includes the following steps: collecting continuous data on temperature, humidity, airflow velocity, and heat flow distribution within the fermentation chamber and overlaying them with time markers; simultaneously recording the heating and ventilation response times to form a dynamic reference; tracking heat flow changes based on the time difference between heating and ventilation stages and extracting the convergence point, extending the heating cycle to form a time misalignment; adjusting the ventilation trigger interval in conjunction with the time misalignment buffer to couple and match air inflow with heat energy attenuation, constructing a stable heat exchange state; and implementing temporal perturbations based on this state, introducing delay differences to maintain asynchronous balance. This invention, through multi-parameter time correlation and temporal control, enables stable misalignment between temperature control and ventilation, suppressing heat flow resonance and stabilizing the temperature field; and maintains long-term asynchronous balance through temporal perturbations, ensuring stable microbial metabolism and improving the conversion efficiency of distiller's grains protein and the overall stability of the fermentation process.

The present application relates to a kind of spent grain protein peptide and its preparation method and application. Specifically, with beer brewing by-product spent grain as raw material, it is prepared by protease enzymolysis to prepare spent grain enzymolysis oligopeptide, and it is proved by mouse in-vivo experiment that the enzymolysis complex has the function of improving blood sugar, obesity, blood fat. On this basis, from the enzymolysis product, the key blood sugar reducing active peptide segment FPQPPQ, FPQQPPFG, FPQPQQPFP is identified, and it is verified that its alpha amylase inhibition rate is superior to the same concentration of acarbose. The spent grain protein peptide complex and spent grain protein peptide of the present application have the effect of reducing blood sugar, and can be applied to the development of food, health food, medicine and the like.

This invention discloses a primer combination for water-saving and quality screening of winter wheat and its application, relating to the field of molecular biology. The primer combination includes primer pair P1 and primer pair P2. This primer combination is used for molecular marker screening of water-saving and drought-resistant traits in winter wheat and can be combined with SDS-PAGE electrophoresis to detect grain protein subunit composition for screening quality traits in winter wheat. This invention designs specific primer combinations, constructs a molecular marker screening system, and innovatively combines it with the "pre-irrigation followed by drought" cultivation management model, forming a highly efficient breeding method suitable for winter wheat. This method avoids dependence on a single known gene, instead achieving targeted improvement of water-saving, drought-resistant, and quality through synergistic screening of primer combinations and environmental adaptability breeding, providing a new technical path for wheat breeding in the arid North China region.

The invention discloses application of soybean GmAGL1 in changing soybean grain protein components and increasing sulfur-containing amino acid content. The nucleotide sequence of the transcription factor coding gene GmAGL1 of the soybean MADS box is as shown in SEQ ID NO. 1. The constructed plant overexpression vector pBA002-GmAGL1 is subjected to agrobacterium tumefaciens-mediated soybean genetic transformation to obtain a soybean receptor variety Jack, and it is found that compared with a receptor control group, the 7S component content and the sulfur-containing amino acid content in seeds of an overexpression transgenic soybean plant are increased, and the quality of the seeds of an overexpression transgenic soybean line is improved. Therefore, the gene can be introduced into plants as a target gene to improve the sulfur-containing amino acid content of soybean seeds. Therefore, a new function of the transcription factor coding gene GmAGL1 of the soybean MADS box in soybean plants is found, and protein components are changed through genetic engineering, so that the application in the aspect of transgenic soybean grain quality is influenced.

The invention discloses a preparation method and application of peony seed protein with high surface hydrophobicity. The method comprises the following steps: washing and drying peony seeds, carrying out superfine grinding treatment, removing grease through a squeezing method, mixing peony seed meal with distilled water according to a certain proportion, carrying out homogenization pretreatment, adopting an ultrasonic physical field superposition auxiliary alkali extraction method, and carrying out operations of multiple times of acid precipitation, redissolution, centrifugation, dialysis and the like, so as to obtain the peony seed meal. And finally freeze-drying to obtain powdery peony seed protein. The peony seed protein obtained by the method has high surface hydrophobicity, the foam property of the protein is remarkably improved, and the protein yield is also remarkably increased. A new technical route is provided for extraction and preparation of protein with high surface hydrophobicity in plant source grain protein, high-quality novel plant protein resources are provided for industries such as food, medicine and cosmetics, and the method has wide application prospects and market potential.

The invention discloses an application of a soybean GmCIPK3 (GmCIPK3) protein coding gene GmCIPK3. The nucleotide sequence of the soybean GmCIPK3 protein coding gene GmCIPK3 is as shown in SEQ ID NO. 1. The constructed plant overexpression vector pBA002-GmCIPK3 is subjected to agrobacterium tumefaciens-mediated soybean genetic transformation to obtain a soybean receptor variety Tianlong, and it is found that compared with a receptor control group, the protein content in seeds of an overexpression transgenic soybean plant is increased, the oil content is reduced, and the protein quality of the seeds of the overexpression transgenic soybean plant is improved. Therefore, the gene can be introduced into plants as a target gene to improve the quality of soybean grain protein. Therefore, the new function of the soybean GmCIPK3 protein coding gene GmCIPK3 in soybean plants is found, the protein content and the oil content are changed through genetic engineering, and then the application in the aspect of transgenic soybean grain quality is affected.

This invention belongs to the field of molecular biology and genetic breeding technology, and discloses a QTL related to soybean seed protein content. qPro12 Tightly linked molecular markers and their applications. A genome-wide association study (GWAS) was used to identify a seed protein content QTL on soybean chromosome 12. qPro12 The significantly associated SNP is located at position 7,757,075 on chromosome 12 of the reference genome Glycine_max_v2.1, and can explain 0.51% of the phenotypic variation in the associated population. PARMS markers developed using this SNP were used to detect genotyping in 256 soybean varieties, demonstrating clear typing and ease of operation, making it suitable for molecular breeding of soybean grain protein content.

The invention belongs to the technical field of agriculture, and particularly relates to a molecular breeding method for improving wheat quality and application. According to the invention, key genetic regulation and control sites of kernel quality characters such as grain protein content, grain hardness and HMW-GS are integrated, a set of efficient wheat quality molecular marker selection system is constructed, and the invention aims to provide technical support for wheat quality precise breeding and assist efficient cultivation of high-quality wheat varieties.

The invention discloses a gene Dv-LMW10 for improving the processing quality of wheat bread and application of the gene Dv-LMW10. The CDS sequence of the haynaldia villosa Dv-LLW10 gene is as shown in SEQ ID NO: 1, the Dv-LLW10 genome sequence and the upstream 2000bp promoter sequence are as shown in SEQ ID NO: 2, and the coding amino acid sequence of the Dv-LLW10 gene is as shown in SEQ ID NO: 3. The Dv-LMW10 gene is converted into wheat under the driving of a promoter of the Dv-LMW10 gene and has the effect of obviously increasing the grain protein content, and the processing quality indexes such as the wet gluten content, the sedimentation value and the weakening degree are obviously superior to those of background and negative control materials. Therefore, the gene provided by the invention can be combined with a self promoter and then introduced into a proper expression vector and transformed into a plant host, so that the content of grain protein is changed, and the processing quality of wheat bread is improved.

The invention discloses an application of OsFTL1 and a coding gene thereof in improving protein content and chlorophyll content. The OsFTL1 disclosed by the invention is derived from rice and is a protein as shown in SEQ ID No. 1. Experiments prove that the OsFTL1 and the coding gene thereof can increase the chlorophyll content and the protein content, and under the normal nitrogen condition, the contents of chlorophyll a, chlorophyll b and carotenoid of leaves of the OsFTL1 transgenic plant are higher than those of wild plants; under the condition of low nitrogen, the leaf protein content of the OsFTL1 transgenic plant is obviously higher than that of a wild type; after the plants growing in the field are harvested, the grain protein content of the OsFTL1 transgenic plants is obviously higher than that of the wild plants. The results show that the OsFTL1 and the coding gene thereof can promote nitrogen assimilation, increase pigment synthesis and increase protein content, and have good application prospects.

The present invention belongs to the technical field of molecular markers and millet genetic breeding, and specifically relates to a molecular marker related to the protein content of millet grains, a KASP primer pair and an application thereof. The molecular marker provided by the present invention is located at 8815143bp on chromosome 3 of the millet genome, and the single nucleotide polymorphism is C / G; when the genotype of millet is CC or CG, it has a high grain protein content trait; when the genotype of millet is GG, it has a low grain protein content trait. The molecular marker described in the present invention is related to the protein content of millet grains, and can distinguish millets with high grain protein content from millets with low grain protein content. It is effective for identifying the protein content trait of millet grains, and can be applied to millet breeding and high-quality genetic improvement application research, providing a new choice for breeding applications to increase the protein content of millet.

The present invention discloses a device for extracting and separating protein from grains. The present invention relates to the technical field of protein extraction and separation, and comprises a wrapping shell, wherein the outer surface of the wrapping shell is fixedly connected to a connecting plate, and the connecting plate is fixedly connected to a frame on a side away from the wrapping shell; the upper surface of the frame is provided with a shaking mechanism, and by providing the shaking mechanism, the grains that need protein extraction can be shaken, and rice husks or impurities in the grains can be removed during the shaking process, thereby preventing rice husks from being mixed in when the grains are ground, thereby achieving the effect of increasing the protein extraction rate; the shaking mechanism comprises a first fixed frame, the first fixed frame passes through the outer side surface of the wrapping shell, the inner cavity of the first fixed frame is fixedly connected to a track rod, and the outer surface of the track rod is slidably connected to a sliding frame, thereby achieving the effect of removing rice husks and residues in the grains and extracting protein.

The invention discloses a wheat grain protein content regulating gene TaPK-1B as well as an encoding protein and application thereof. The gene has a nucleotide sequence as shown in SEQ ID No.3, or a coding nucleotide sequence which is obtained by substitution, deletion or insertion of one or more nucleotides of the nucleotide sequence as shown in SEQ ID No.3 and has the same functional protein. The wheat TaPK-1B gene is excavated through whole genome association analysis, the wheat TaPK-1B gene is knocked out through genetic transformation, the wheat grain protein content can be increased, the function of the gene for negatively regulating the grain protein content is defined, a new gene resource is provided for wheat quality improvement, and an effective way is provided for wheat molecular breeding.