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Method for determining grain protein

A method of determination and protein technology, which is applied in the field of determination of grain protein, can solve the problems of long time-consuming, cumbersome operation, large amount of digestion catalyst, etc., and achieve the effect of easy operation and accurate measurement

Inactive Publication Date: 2014-11-26
HUBEI FORBON TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this method has the following disadvantages: concentrated H 2 SO 4 —The two processes of catalyst digestion and Kjeldahl nitrogen determination are time-consuming, cumbersome to operate, and consume a large amount of digestion catalyst

Method used

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  • Method for determining grain protein
  • Method for determining grain protein
  • Method for determining grain protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: a kind of assay method of cereal protein

[0021] Weigh 0.1152g rice sample and place it in a 50ml digestion tube, add 5ml concentrated H 2 SO 4 , placed on a far-infrared digestion furnace, heated at 80-100 ° C, until concentrated H 2 SO 4 Decompose and emit white smoke and raise the temperature until the solution turns brown-black, remove the digestion tube from the digestion furnace, cool for 1 minute, add 300g / LH drop by drop 2 o 2 10 drops, shake well, heat on the digestion stove for 10 minutes, remove and cool for 1 minute, then add 300g / LH 2 o 2 10 drops, repeat this way 2-3 times until the cooking solution is colorless or clear, then heat for 10 minutes to remove excess H 2 o 2 , that is, the digestion is completed; transfer the digestion solution to a 100ml volumetric flask, and filter after constant volume; accurately draw 1ml of the filtrate into a 50ml colorimetric tube, add an appropriate amount of water, adjust it to neutral with NaOH ...

Embodiment 2

[0022] Embodiment 2: a kind of assay method of cereal protein

[0023] Weigh 0.1232g corn sample and place it in a 50ml chemical tube, add 5ml concentrated H 2 SO 4 , placed on a far-infrared digestion furnace, heated at 80-100 ° C, until concentrated H 2 SO 4 Decompose and emit white smoke and increase the temperature until the solution turns brown-black, remove the digestion tube from the digestion furnace, cool for 5 minutes, add 300g / L H 2 o 2 10 drops, shake well, heat on the digestion stove for 10 minutes, remove and cool for 5 minutes, then add 300g / L H 2 o 2 5 drops, repeat this way 2-3 times until the cooking solution is colorless or clear, then heat for 5 minutes to remove excess H 2 o 2 , that is, the digestion is completed; transfer the digestion solution to a 100ml volumetric flask, and filter after constant volume; accurately draw 1ml of the filtrate into a 50ml colorimetric tube, add an appropriate amount of water, adjust it to neutral with NaOH solution,...

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Abstract

The invention discloses a method for determining grain protein. The method comprises the following specific steps: putting a certain quantity of grain samples into a 50ml digestive tube; performing digestion on the samples in the presence of concentrated H2SO4-H2O2 in a digestion furnace until a solution is clear bright; transferring digestion liquor into a 100ml volumetric flask, filtering, and determining filter liquor with a Nessler method. The method has the advantages of high measurement precision and easiness and convenience in operation and can be suitable for detecting the protein content of various grains.

Description

technical field [0001] The invention relates to a method for measuring grain protein. Background technique [0002] Protein is an important indicator to determine the edible quality of grains. Currently, the commonly used national standard method uses concentrated H 2 SO 4 — Catalyst digestion, determined by Kjeldahl method. But this method has the following disadvantages: concentrated H 2 SO 4 —The two processes of catalyst digestion and Kjeldahl nitrogen determination are time-consuming, cumbersome to operate, and a large amount of digestion catalyst is used. In production and scientific research, in order to complete the determination of a large number of samples, it is necessary to adopt a fast and simple analysis method. At present, there are many rapid determination methods of grain protein reported in the literature, such as biuret colorimetric method, dye binding method, and ninhydrin method. Contents of the invention [0003] The purpose of the present inven...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
Inventor 蔚霞王仁宗刘裕王瑞
Owner HUBEI FORBON TECH
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