8 results about "Protein formation" patented technology

The application provides a DNA virus tracer coated with a protein by an EDC covalent coupling method and a preparation method and application thereof. The DNA virus tracer coated with the protein of the application is composed of a core, a shell and a protein shell coated on the shell; the core is a SiO2 nanosphere with adsorbed nucleic acid on the surface, a layer of SiO2 shell is coated on the outside of the core, the surface of the shell is modified by carboxylation, and the shell is formed by covalently coupling the protein through EDC. The novel DNA virus tracer provided by the application can effectively protect the coding DNA, facilitate subsequent quantitative detection and analysis, effectively ensure that the outermost shell of the tracer is completely composed of the selected protein, and make the surface characteristics and migration characteristics of the tracer closer to the target virus.

The invention discloses an electrochemical sensor for glycoprotein detection and a glycoprotein detection method. The method comprises the following steps: anchoring an aptamer-protein conjugate on the surface of a gold electrode, and then assembling an anti-fouling peptide around the aptamer-protein conjugate to form an imprinted self-assembled monolayer film. And removing the combined protein through an acid solution to form a biocompatible imprinting cavity capable of being used for recombining a target object. The imprinted cavity can eliminate non-specific adsorption and enhance targeted binding efficiency. The sensor can be used for directly detecting carcino-embryonic antigens with the concentration as low as 0.1 ng / mL through an electrochemical impedance spectroscopy method. Besides, homodimer concanavalin A (ConA) is used as a recognition element and a cross-linking agent, homodimer glucose oxidase (GOx) is assembled on the surface of the electrode in situ, a protein network can be formed, and enzyme catalysis signal amplification detection is achieved. Through in-situ formation of a ConA-GOx assembly, the sensitivity is improved by 100 times.

The invention discloses a protein targeted degradation system based on a biological orthogonal protein pair and application of the protein targeted degradation system, and belongs to the field of biological medicine. Wherein the protein targeting degradation system comprises a ligand A targeting a tumor specific receptor, a ligand B targeting a tumor target protein and a biological orthogonal protein pair, and the ligand A and the ligand B respectively form a fusion protein with one protein in the biological orthogonal protein pair. The targeted degradation system provided by the invention can realize precise, efficient and safe degradation of tumor-related proteins (such as EGFR, PD-L1, HER2, c-MET and the like) through dual precise recognition of the targeting ligand and the bio-orthogonal protein pair, thereby realizing anti-tumor treatment.

The present invention relates to method for purifying proteins naturally occurring in mammalian milk from aqueous protein solution using water-soluble silicate. The silicates form an insoluble precipitate of a silicate-protein complex from which the proteins can be isolated.

This invention belongs to the medical field and provides a calcium carbonate manganese nanoprobe for in situ imaging detection of vascular epidermal growth factor, its preparation method, and its application. In this strategy, a DNA strand (P1 and P2) containing a protein recognition antibody, a protein recognition sequence, and a deoxyribonuclease, along with a molecular beacon DNA strand (H1), is stirred in a DMEM cell culture medium containing calcium chloride and manganese chloride to prepare the calcium carbonate manganese nanoprobe. In acidic tumor cells, this nanoprobe releases P1, P2, H1, and manganese ions. P1 and P2 can simultaneously recognize proteins, forming a complete deoxyribonuclease center, which catalyzes the cleavage of H1 under the action of the cofactor manganese ions, generating a fluorescent signal. This strategy achieves specific in situ dynamic imaging of low-abundance proteins in vivo and has great potential in biomedical research.

The application discloses an amyloid configuration optical detection method based on infrared waveband multi-frequency resonance, which comprises the following steps: obtaining an optical super-structure surface unit; adding an amyloid solution to be detected; irradiating incident light to realize electromagnetic field excitation; detecting the refractive index and layer thickness of a protein formation layer in a groove of a coupling complementary opening resonance ring working in a near-infrared waveband to obtain one-dimensional structure information of the amyloid formation layer to be detected; detecting the component molecule configuration in the groove of the coupling complementary opening resonance ring working in a mid-infrared waveband by using a characteristic absorption peak of a molecule to obtain structure information of the amyloid molecule to be detected; and determining the configuration analysis result of the amyloid to be detected and the relationship between the protein formation layer and the protein component molecule configuration by using the two aspects of information. The application realizes the measurement based on the medium refractive index and the measurement based on the molecular absorption spectrum on the same sensing surface, can realize the protein configuration analysis and organically connects the layer structure and the molecule configuration.

The invention discloses a production method for improving sauce consistency based on protein micelles, and belongs to the technical field of food processing. Aiming at the problems of unnatural texture, unclean label and the like caused by the use of a chemical thickening agent in the existing sauce, the preparation method comprises the following steps: shearing vegetable protein and water at a specific temperature at a high speed to form a hydration solution, adjusting the pH value, and standing for hydration; mixing the protein hydrated liquid with the non-salt and non-acid base material of the sauce; then carrying out thermal induction micellization treatment at 85-95 DEG C to enable the protein to form a stable three-dimensional network structure; after the system is cooled, adding edible salt, table vinegar and other flavoring agents; and finally, homogenizing, degassing, sterilizing and filling to obtain a finished product. Through an innovative process sequence, the technical problem of application of protein in a complex sauce system is successfully solved, and the obtained sauce has the characteristics of smooth texture, good adhesiveness and good stability, completely meets the requirements of clean labels, and is particularly suitable for production of seasoning sauce products such as barbecue sauce and curry sauce.

The invention provides a sustained-release protein gel and a preparation method thereof, and belongs to the field of biological medicine, and specifically, the preparation method of the sustained-release protein gel provided by the invention comprises the following steps: S1, adding protein into cross-linked sodium hyaluronate to form a primary mixture, the protein is selected from at least one of type I bovine collagen, human serum albumin and egg white lysozyme, and the cross-linked sodium hyaluronate is sodium hyaluronate cross-linked by a terminal epoxy group cross-linking agent; s2, performing high-pressure homogenization on the primary mixture in the step S1 to obtain gel particles with uniform particle size; and S3, carrying out static adsorption equilibrium on the gel particles in the step S2 to obtain the sustained-release protein gel. The sustained-release protein gel prepared by adopting the preparation method does not generate a Tyndall effect, and has the advantages of high strength and low adverse reaction occurrence rate.