27 results about "Protein formation" patented technology

The invention discloses a dipeptide modified polymer and application thereof in intracellular delivery of protein. The screened polymer and protein form a compound and are subjected to phase transformation, solute in a liquid phase is transformed into solid-phase particles insoluble in water, early release of the target protein is reduced, and delivery of the protein in the presence of serum is achieved. The protein intracellular delivery method provided by the invention has relatively high intracellular delivery efficiency in the presence of serum-free protein and serum, and the delivery efficiency is higher than that of a commercial protein transfection reagent PULSin; after beta-Gal and other enzyme proteins are delivered into cells, the activity can be recovered, a catalytic substrate is converted into a monitorable product, and the amount of the delivered and active recovered protein is less affected by serum protein in incubation conditions; in addition, the material has low cytotoxicity, the cell activity is higher than 90% under the experimental condition of protein delivery, and the material has good biocompatibility.

The application provides a DNA virus tracer coated with a protein by an EDC covalent coupling method and a preparation method and application thereof. The DNA virus tracer coated with the protein of the application is composed of a core, a shell and a protein shell coated on the shell; the core is a SiO2 nanosphere with adsorbed nucleic acid on the surface, a layer of SiO2 shell is coated on the outside of the core, the surface of the shell is modified by carboxylation, and the shell is formed by covalently coupling the protein through EDC. The novel DNA virus tracer provided by the application can effectively protect the coding DNA, facilitate subsequent quantitative detection and analysis, effectively ensure that the outermost shell of the tracer is completely composed of the selected protein, and make the surface characteristics and migration characteristics of the tracer closer to the target virus.

Structure-based drug design using a computer, includes: identifying a protein target of pharmacological interest; identifying at least three different ligands for binding to the protein; for each of the ligands, determining a relative strength of binding between the ligand and the protein to form a corresponding complex; ranking the different ligands identified as forming complexes with the protein based on the determined relative binding free energies; and identifying one or more of the ranked ligands as candidates for the drug based on the ranking. Determining the relative strength includes: simulating, using the computer, a set of pairs of different ligands forming at least one closed thermodynamic cycle comprising a plurality of legs linking at least two different ligand pairs to determine multiple relative binding free energy differences for the set of pairs of different ligands; and determining, using the computer, a non-zero hysteresis magnitude associated with each closed thermodynamic cycle by summing the relative binding free energy differences for each of the ligand pairs that form a closed thermodynamic cycle.

The invention discloses a protein-based adsorbent for selectively adsorbing metal rhenium ions and its application. The adsorbent is obtained by encapsulating alkylamine or ammonium salt in a phase transition aggregate formed by reducing protein with a reducing agent. The method of using the adsorbent is to put the adsorbent into a mixed solution containing rhenium and molybdenum to achieve selective adsorption of rhenium. The preparation and use methods of the adsorbent are simple, environmentally friendly, recyclable, and have a high selective adsorption capacity for rare metal rhenium. The adsorbent can be used for the separation of rhenium and molybdenum in industrial wastewater and ore leachate, and after adsorption-desorption cycles, it still has a high adsorption ratio.

There are disclosed therapies and preventions of prion protein complex infections. The transcription of the amyloid precursor protein gene and PrP gene and the RNA transcript are the rate-limiting steps and are most susceptible for blockage and control of the process of amyloid protein formation and PrPsc formation. Thus, therapies and prevention regimes for prion protein complex infections interrupt this process at the level of DNA transcription to RNA, RNA transport to the mitochondrion for protein synthesis and deposition in the cerebral cortex neurons.

The invention discloses an electrochemical sensor for glycoprotein detection and a glycoprotein detection method. The method comprises the following steps: anchoring an aptamer-protein conjugate on the surface of a gold electrode, and then assembling an anti-fouling peptide around the aptamer-protein conjugate to form an imprinted self-assembled monolayer film. And removing the combined protein through an acid solution to form a biocompatible imprinting cavity capable of being used for recombining a target object. The imprinted cavity can eliminate non-specific adsorption and enhance targeted binding efficiency. The sensor can be used for directly detecting carcino-embryonic antigens with the concentration as low as 0.1 ng / mL through an electrochemical impedance spectroscopy method. Besides, homodimer concanavalin A (ConA) is used as a recognition element and a cross-linking agent, homodimer glucose oxidase (GOx) is assembled on the surface of the electrode in situ, a protein network can be formed, and enzyme catalysis signal amplification detection is achieved. Through in-situ formation of a ConA-GOx assembly, the sensitivity is improved by 100 times.

The invention discloses a protein targeted degradation system based on a biological orthogonal protein pair and application of the protein targeted degradation system, and belongs to the field of biological medicine. Wherein the protein targeting degradation system comprises a ligand A targeting a tumor specific receptor, a ligand B targeting a tumor target protein and a biological orthogonal protein pair, and the ligand A and the ligand B respectively form a fusion protein with one protein in the biological orthogonal protein pair. The targeted degradation system provided by the invention can realize precise, efficient and safe degradation of tumor-related proteins (such as EGFR, PD-L1, HER2, c-MET and the like) through dual precise recognition of the targeting ligand and the bio-orthogonal protein pair, thereby realizing anti-tumor treatment.

A disease detection system including a platform or cartridge to perform testing using a rapid lateral flow chromatographic immunoassay (LFIA) test intended for the qualitative detection of the disease in a sample, such as collected sputum, disrupted by a digestive enzyme and a detergent to release its content and digest its proteins and other polymeric molecules. A method of the present invention includes treating a sample with a digestive enzyme and a detergent to disrupt the collected sample releasing content and digesting proteins of the sample to form a lysed-digested-extracted sample, applying the lysed-digested-extracted sample to a platform or cartridge, performing a rapid lateral flow chromatographic immunoassay (LFIA) test of the lysed-digested-extracted sample using the platform or cartridge and detecting presence or absence of an analyte of interest from the tested lysed-digested-extracted sample.

The present invention relates to method for purifying proteins naturally occurring in mammalian milk from aqueous protein solution using water-soluble silicate. The silicates form an insoluble precipitate of a silicate-protein complex from which the proteins can be isolated.

This invention belongs to the medical field and provides a calcium carbonate manganese nanoprobe for in situ imaging detection of vascular epidermal growth factor, its preparation method, and its application. In this strategy, a DNA strand (P1 and P2) containing a protein recognition antibody, a protein recognition sequence, and a deoxyribonuclease, along with a molecular beacon DNA strand (H1), is stirred in a DMEM cell culture medium containing calcium chloride and manganese chloride to prepare the calcium carbonate manganese nanoprobe. In acidic tumor cells, this nanoprobe releases P1, P2, H1, and manganese ions. P1 and P2 can simultaneously recognize proteins, forming a complete deoxyribonuclease center, which catalyzes the cleavage of H1 under the action of the cofactor manganese ions, generating a fluorescent signal. This strategy achieves specific in situ dynamic imaging of low-abundance proteins in vivo and has great potential in biomedical research.

A servo-driven plate forming machine comprising: a mold plate that operates at (120) 10″ strokes per minute; a single hydraulic pump combined with proportional hydraulic valves with closed and open loop controls; individually controlled feed screws to control whole muscle integrity and retain particle definition; and a one piece hopper that assists in providing a substantially leak proof feed.

The application discloses a kind of fermented citrus dreg insoluble dietary fiber functional emulsion and preparation method and application.The application utilizes composite probiotic fermentation to be combined with composite enzyme processing, obtains modified citrus dreg insoluble dietary fiber, then with protein forms insoluble dietary fiber-protein covalent complex, is treated by ultrasonic and high speed shearing to obtain nutritional emulsion.Compared with prior art emulsion preparation method, the application utilizes fermentation to improve insoluble dietary fiber monosaccharide content, so that it is easy to occur Maillard reaction with protein to form stable covalent complex, and the nutritional ingredient delivery system droplet prepared by using the same as emulsifier is small, and environmental tolerance is strong, solve the problems such as poor emulsion stability, easy to aggregate, low encapsulation efficiency, improve the bioavailability of nutritional ingredients, and enhance the antioxidant activity of emulsion.The fermented citrus dreg insoluble dietary fiber functional emulsion obtained by the foregoing preparation method is a good biological delivery carrier, and can be applied in food, cosmetics and pharmaceuticals and other fields.

The invention provides a method for stabilizing fermented buffalo milk, which comprises the following steps: firstly, adjusting the pH value of buffalo milk before thermal sterilization to 7.4 from about 6.7 in a natural state, so that the chemical environment of the product in a high-temperature sterilization process can be effectively improved; secondly, TG enzyme is added while the white granulated sugar is added into the buffalo milk system, the TG enzyme can catalyze cross-linking reaction in protein molecules or among the molecules, and in the fermented buffalo milk system, the protein is promoted to form a more stable and ordered network structure. According to the invention, the two key problems of mouth feel and shelf life in the production process of the normal-temperature fermented buffalo milk are fundamentally solved, the market competitiveness of the product is greatly improved, and the preparation method has extremely high application value and economic benefit.

To provide a novel protein for facilitating the stabilization or structural analysis of a target protein.SOLUTION: Provided is a protein for addition to a target protein that forms a predetermined tertiary structure including a central domain, a first peripheral domain, and a second peripheral domain, the central domain, the first peripheral domain, and the second peripheral domain each including a hydrophobic core formed by a plurality of helices, the hydrophobic core of the central domain being formed by a plurality of helices including at least one helix included in the first peripheral domain and at least one helix included in the second peripheral domain.SELECTED DRAWING: Figure 1A

The present invention relates to a method for producing a gelation-enhanced plant protein isolate derived from legumes such as mung beans, chickpeas, lentils, and peas. The process involves milling, dry air classification, alkaline extraction with anti-foaming agents, and protein recovery via centrifugation or filtration, optionally followed by spray drying. The protein isolate is enzymatically treated with Glucose Oxidase to improve gelation properties, then further enhanced by calcium lactate and optional natural additives like quillaia extract and glucono delta lactone. The resulting protein isolate contains at least 80% protein on a dry weight basis, forms thermally reversible or irreversible gels, and excludes synthetic crosslinkers, emulsifiers, or animal-derived ingredients, supporting clean-label applications. Additionally, the invention covers methods of incorporating this protein isolate into plant-based food formulations and processing them to yield final products with superior textural attributes including firmness, cohesiveness, elasticity, and mouthfeel.

The application discloses an amyloid configuration optical detection method based on infrared waveband multi-frequency resonance, which comprises the following steps: obtaining an optical super-structure surface unit; adding an amyloid solution to be detected; irradiating incident light to realize electromagnetic field excitation; detecting the refractive index and layer thickness of a protein formation layer in a groove of a coupling complementary opening resonance ring working in a near-infrared waveband to obtain one-dimensional structure information of the amyloid formation layer to be detected; detecting the component molecule configuration in the groove of the coupling complementary opening resonance ring working in a mid-infrared waveband by using a characteristic absorption peak of a molecule to obtain structure information of the amyloid molecule to be detected; and determining the configuration analysis result of the amyloid to be detected and the relationship between the protein formation layer and the protein component molecule configuration by using the two aspects of information. The application realizes the measurement based on the medium refractive index and the measurement based on the molecular absorption spectrum on the same sensing surface, can realize the protein configuration analysis and organically connects the layer structure and the molecule configuration.

Embodiments of the present application provide a method and apparatus for model training, drug screening, and affinity prediction. Embodiments of the present application provide a method for predicting the affinity between a small molecule compound and a protein, which includes: determining an access region based on the three-dimensional conformation of a complex formed by the small molecule compound to be analyzed and the protein; constructing a topological graph G based on the characteristics of atoms and chemical bonds within the access region; determining a feature vector based on the topological graph G; and using a trained machine learning model to process the feature vector to obtain the affinity between the compound and the protein. Using this method can improve the efficiency, interpretability, repeatability, accuracy, and precision of predicting the affinity between a small molecule compound and a protein.

Provided is a method for producing a heterodimer protein, which is also capable of producing a heterodimer protein comprising only a domain comprising a natural amino acid sequence, for example, a heterodimer protein such as a bispecific antibody. This production method is a method for producing a heterodimer protein, comprising a production step for producing a heterodimer protein by reacting a dimer protein containing a reaction tag with a modified protein that modifies the dimer protein, the reaction tag-containing dimer protein comprises a first monomeric protein comprising, in order, a first reaction tag comprising a binding tag and a first C-intein and a first dimer-forming domain capable of forming a dimer, and a second monomeric protein comprising, in order, a second reaction tag comprising a binding tag and a second C-intein, and a second monomeric protein comprising, in order, a second reaction tag comprising a binding partner capable of binding to the binding tag and a second C-intein and a second dimer-forming domain capable of forming a dimer with the first dimer-forming domain, the modified proteins comprise a first modified protein comprising a first N-intein capable of reacting with the first C-intein and a first adduct attached to the first monomeric protein, and a second modified protein comprising a second N-intein capable of reacting with the second C-intein. And a second modified protein comprising a second N-intein capable of reacting with the second C-intein and a second adduct attached to the second monomeric protein, the first adduct and the second adduct being different adducts, the first monomeric protein and the second monomeric protein forming a dimer, in the production step, the first N-intein of the first modified protein reacts with the first C-intein of the first monomeric protein such that a first adduct of the first modified protein is linked to the first monomeric protein. The second N-intein of the second modified protein reacts with a second C-intein of the second monomeric protein such that a second adduct of the second modified protein is linked to the second monomeric protein.

There are disclosed therapies and preventions of prion protein complex infections. The transcription of the amyloid precursor protein gene and PrP gene and the RNA transcript are the rate-limiting steps and are most susceptible for blockage and control of the process of amyloid protein formation and PrPsc formation. Thus, therapies and prevention regimes for prion protein complex infections interrupt this process at the level of DNA transcription to RNA, RNA transport to the mitochondrion for protein synthesis and deposition in the cerebral cortex neurons.

The invention discloses a production method for improving sauce consistency based on protein micelles, and belongs to the technical field of food processing. Aiming at the problems of unnatural texture, unclean label and the like caused by the use of a chemical thickening agent in the existing sauce, the preparation method comprises the following steps: shearing vegetable protein and water at a specific temperature at a high speed to form a hydration solution, adjusting the pH value, and standing for hydration; mixing the protein hydrated liquid with the non-salt and non-acid base material of the sauce; then carrying out thermal induction micellization treatment at 85-95 DEG C to enable the protein to form a stable three-dimensional network structure; after the system is cooled, adding edible salt, table vinegar and other flavoring agents; and finally, homogenizing, degassing, sterilizing and filling to obtain a finished product. Through an innovative process sequence, the technical problem of application of protein in a complex sauce system is successfully solved, and the obtained sauce has the characteristics of smooth texture, good adhesiveness and good stability, completely meets the requirements of clean labels, and is particularly suitable for production of seasoning sauce products such as barbecue sauce and curry sauce.

The invention discloses curcumin-embedded red bean protein-corn starch composite condensation gel as well as a preparation method and application thereof, and belongs to the technical field of functional food processing. In order to solve the technical problems that curcumin is relatively low in stability and bioavailability, and condensation gel only formed by protein cannot meet the application requirement of being used as a curcumin carrier, the invention provides a preparation method of curcumin-embedded red bean protein-corn starch composite condensation gel. Sequentially carrying out mixing co-heating and ultrahigh pressure treatment on red bean protein subjected to pH shift and ultrasonic treatment and corn starch with a specific straight / branched ratio, introducing curcumin into a protein-starch composite system, and carrying out high-pressure microjet homogenization treatment to finally form the composite condensation gel under the acid induction condition. The prepared composite condensation gel has high gel strength and physical and chemical stability, can provide better protection for sensitive bioactive compounds such as curcumin, realizes controlled release of the bioactive compounds and improves the bioaccessibility of the bioactive compounds.

The invention discloses a method for extracting polysaccharide from cucumis metuliferus, and relates to the field of polysaccharide preparation, the extraction method comprises the following steps: S1, adding cucumis metuliferus pulp into an aqueous solution containing an antioxidant, homogenizing, adding an electrolyte solution, and stirring to obtain a cucumis metuliferus homogenate; s2, adding a compound enzyme solution into the horned melon homogenate for enzymolysis to obtain an enzymolysis mixed solution; s3, adding an extraction solvent into the enzymolysis mixed solution for extraction, and performing heat preservation and stirring to obtain an extracting solution; s4, sequentially carrying out solid-liquid separation, adsorption decoloration and deproteinization, ethanol precipitation, washing and drying to obtain cucumis metuliferus polysaccharide powder. By adopting a composite enzymolysis process containing pectinase, cellulase and protease, a mucus colloidal network structure formed by pectin cellulose and protein in the cucumis metuliferus is effectively broken, and compared with the phenomena of polysaccharide structure damage and glucosidic bond breakage caused by extraction of cucumis metuliferus polysaccharide by a traditional extraction method, the extraction method has the advantages that the extraction efficiency is improved; the extraction efficiency and activity retention of the cucumis metuliferus polysaccharide can be effectively improved.

The invention provides a sustained-release protein gel and a preparation method thereof, and belongs to the field of biological medicine, and specifically, the preparation method of the sustained-release protein gel provided by the invention comprises the following steps: S1, adding protein into cross-linked sodium hyaluronate to form a primary mixture, the protein is selected from at least one of type I bovine collagen, human serum albumin and egg white lysozyme, and the cross-linked sodium hyaluronate is sodium hyaluronate cross-linked by a terminal epoxy group cross-linking agent; s2, performing high-pressure homogenization on the primary mixture in the step S1 to obtain gel particles with uniform particle size; and S3, carrying out static adsorption equilibrium on the gel particles in the step S2 to obtain the sustained-release protein gel. The sustained-release protein gel prepared by adopting the preparation method does not generate a Tyndall effect, and has the advantages of high strength and low adverse reaction occurrence rate.

There are disclosed therapies and preventions of prion protein complex infections. The transcription of the amyloid precursor protein gene and PrP gene and the RNA transcript are the rate-limiting steps and are most susceptible for blockage and control of the process of amyloid protein formation and PrPsc formation. Thus, therapies and prevention regimes for prion protein complex infections interrupt this process at the level of DNA transcription to RNA, RNA transport to the mitochondrion for protein synthesis and deposition in the cerebral cortex neurons.

Methods of treating and preventing prion protein complex infection are disclosed. Transcription of an amyloid protein precursor protein gene and a PrP gene and RNA transcription are speed limiting steps, and the process of amyloid protein formation and PrPsc formation is most easily blocked and controlled. Thus, the regimen for the treatment and prevention of prion protein complex infection interrupts this process at the level of DNA transcription to RNA, RNA transport to mitochondria for protein synthesis and deposition at cerebral cortical neurons.

A gel forming combination, and textured vegetable protein blended therewith, the gel forming combination comprising a first and second plant proteins; wherein, the first plant protein comprises at least one protein or is a protein selected from the group consisting of potato protein and patatin and combination of same; wherein said second plant protein comprises one or more proteins other than potato protein or patatin; and wherein a combination gel, thermally produced from said first plant protein, second plant protein and water; has a hardness that is statistically significantly greater than a hardness of a single protein gel, formed from a single protein of said first plant protein or of said second plant protein, the amount of said single protein in the single protein gel being identical to total proteins amount in said combination gel. Also provided are alternative meat products comprising a gel from the disclosed gel forming combination.

To provide a protein delivery agent and an intracellular protein introduction method capable of efficiently transporting proteins into cells and / or nuclei without showing cytotoxicity.SOLUTION: An intracellular protein delivery agent is a complex formed by at least cationic carrier molecules and anionic carrier molecules, which are bonded to each other by at least ionic bonds and hydrophobic interactions. Preferably, the complex is formed by cationic carrier molecules, anionic carrier molecules, and a protein, which are bonded to each other by at least ionic bonds and hydrophobic interactions. An intracellular protein introduction method uses the intracellular protein delivery agent. Here, the intracellular protein delivery agent may be an oligomer or polymer in which the cationic carrier molecule has a cationic group as a repeating unit.SELECTED DRAWING: Figure 1