49 results about "Binding selectivity" patented technology

X-ray fluorescence (XRF) spectrometry has been used for detecting binding events and measuring binding selectivities between chemicals and receptors. XRF may also be used for estimating the therapeutic index of a chemical, for estimating the binding selectivity of a chemical versus chemical analogs, for measuring post-translational modifications of proteins, and for drug manufacturing.

A modified antibody molecule which selectively binds to a specific target, the antibody molecule being modified at at least one amino acid residue that determines antigen binding selectivity and/or affinity, characterised in that the modified antibody molecule exhibits a greater stability than an unmodified parent antibody molecule which selectively binds to the target. Also described are nucleotide sequences encoding, vectors, host cells and composition containing and uses of such antibodies, antibody fragments or antibody derivatives.

The invention discloses an affinity chromatography medium employing tetrapeptide as a functional ligand and a preparation method of the affinity chromatography medium. The method comprises the following steps: adding dry matrix and allyl bromide to a dimethyl sulfoxide solution, activating, and reacting activating matrix with N-bromo succinimide; enabling bromo alcoholized matrix to react with hexamethylendiamine to obtain amino activating matrix; sequentially washing with deionized water, absolute ethyl alcohol and anhydrous N,N-dimethyl formamide, adding an N,N-dimethyl formamide solution containing tetrapeptide, 2-(7-azobenzotriazole)-N,N,N',N'-te-tramethyluronium hexafluorophosphate and N,N-diisopropylethylamine, and coupling a tetrapeptide ligand; and putting a medium coupled to tetrapeptide in a mixed liquid of sodium acetate and acetic anhydride to obtain the affinity chromatography medium employing tetrapeptide as the functional ligand. According to the novel chromatography medium developed by the method, a functional group is tetrapeptide composed of tyrosine, phenylalanine, arginine and histidine, and is designed on the basis of a protein A binding site of an antibody Fc segment; the antibody binding selectivity is greatly improved; and the affinity chromatography medium can be applied to efficient separation of an antibody.

Methods and apparatus are provided for using various megasonic apparatus including megasonic tanks, scanning megasonic plates, megasonic jets, and megasonic sweeping beams etc., in combination with selective chemistries to remove sub-micron particulate contaminants from the surfaces of the processing equipment used in semiconductor, medical, or any other processing environments.

The invention discloses purification equipment for tail gas from an acrylonitrile absorption tower. The purification equipment comprises a gas-liquid separator for separating the tail gas from the acrylonitrile absorption tower, an air filter for mixing fresh air and the tail gas, an oxygen supply fan and a heat exchanger for heat exchange of mixed gas of the tail gas and the air; the heat exchanger is circularly communicated with a catalytic reactor, and the mixed gas produced after catalytic oxidation reaction is introduced from the top of a denitration reactor through the heat exchanger; the bottom of the denitration reactor is communicated with a waste heat boiler for recycling heat; and a chimney for exhausting the purified tail gas is arranged at the exhaust port of the waste heat boiler. Through the technical scheme, the purification equipment has the following advantages that: by taking the measures of combining a catalytic oxidation system and a selective catalytic reduction denitration system, adopting segmental heat receiving in the catalytic oxidation reactor, reducing redundant oxygen quantity or adopting segmental combustion, operational stability and safety are ensured, and the organic substances and the nitrogen oxides in the treated tail gas accord with the national discharge standards.

Disclosed are embodiments of a semiconductor wafer processing method that allow device regions to be selectively annealed following back end of the line (BEOL) metal wiring formation without degrading wiring layer reliability. In the embodiments, a semiconductor device is formed adjacent to the top surface of a wafer such that it incorporates a selectively placed infrared absorbing layer (IAL). Then, following BEOL metal wiring formation, the bottom surface of the wafer is exposed to an infrared light having a wavelength that is transparent to the wafer. The infrared light is absorbed by and, thereby heats up the IAL to a first predetermined temperature (e.g., a dopant activation temperature, a temperature required for a state change, etc.). The resulting heat is transferred from the IAL to an adjacent region of the semiconductor device without raising the temperature of the metal wiring above a second predetermined temperature (e.g., a temperature that could degrade the metal wiring) that is lower than the first predetermined temperature.

The present invention provides halogenated calixpyrrole, calixpyridinopyrrole, and calixpyridine macrocycles having 4–12 pyrrolic rings with greater stability, enhanced anion and neutral molecule binding affinity, and different binding selectivites as compared to their non-halogenated congeners as judged from ¹H NMR, ¹⁹F NMR and fluorescence emission spectroscopic analyses.

Phage display was used to screen peptide libraries that distinguish between well-differentiated (HCT116) and poorly-differentiated colon carcinoma cells (HT29). The screening protocol used selection and subtraction on intact, viable cells, resulting in phage libraries exhibiting high binding selectivity for the poorly-differentiated HT29 cells. A nine amino acid, disulfide-constrained peptide (RPM) was identified that selectively bound and was internalized into colon cancer cells. The peptide may be used to detect colon cancer cells and also may be used to selectively deliver therapeutic agents to the cells.

The invention discloses a nitrogen-doped carbon-inlaid non-noble metal catalyst and a preparation method and application thereof. A non-noble metal organic complex and a compound of silicon dioxide are taken as precursors, and the catalyst for efficient decomposition of ammonia-borane into hydrogen by the aid of high temperature thermal decomposition and in combination with a selective etching strategy. The preparation method includes: dispersing the non-noble metal organic complex and commercial silica nanoparticles in an ethanol-chloroform two-phase solvent system and removing the solvent byspin evaporation to obtain composite nanopowder; putting the composite nanopowder in a tube furnace, raising the temperature to 700-1000DEG C under the protection of insert gas and keeping the constant temperature for 1-6 hours; repeatedly treating obtained black solid in a dilute alkali solution for 12-24 hours at the temperature of 50DEG C, and performing bleaching, washing and drying to obtainthe target catalyst. The catalyst shows excellent catalytic properties, resistance to poisioning/inactivation and cycling stability in the catalytic hydrolysis of ammonia-borane to produce hydrogen;the method has the advantages that the raw materials are easy to obtain, preparation period is short, and batch production can be realized.

The present invention relates to a peptide or polypeptide comprising an Fv molecule, a construct thereof, a fragment of both, or a construct of a fragment, which has enhanced binding characteristics to favor selective and/or Or specifically bind to target cells, wherein the binding selectivity or specificity is mainly determined by the first hypervariable region, and wherein the Fv is scFv or dsFv, and optionally has one or more markers. Said enhanced binding is to a substantially exposed and/or overexpressed binding site on or within the target comprising a cell that is substantially Other cells that do not present and/or do not express the binding site are more favorable for binding. The invention also relates to methods for isolating said peptides and polypeptides from phage display libraries, and to nucleic acid molecules encoding them. The present invention provides pharmaceutical compositions comprising said peptides or polypeptides, and kits for the diagnosis and treatment of diseases, preferably cancer, most preferably acute myeloid leukemia.

The present invention relates to the detection of HLA-G. Antibodies to both soluble and membrane bound HLA-G are disclosed. Exemplary antibodies include 2C/C8, 3C/G4, and 4H84. 2C/C8 and 4H84 antibodies bind to the same region of HLA-G, which is a different region than that bound by 3C/G4. Methods of detection and diagnosis are disclosed as well as kits, including a miniaturized assay suitable for a clinical setting. Further, a method of selecting an embryos for in vitro fertilization is disclosed. A sandwich ELISA test is provided using two antibodies that bind to HLA-G at different regions. The HLA-G test according to the invention is over 1000 times more selective in binding to HLA-G than to antigens HLA-A2, HLA-B4, HLA-C, or mixed WBC preparations.

Described herein are cyclic peptides, nanoparticles bound with cyclic peptides, and methods for using said cyclic peptides and/or said nanoparticles bound with cyclic peptides for intraoperative nerve tissue imaging.

X-ray fluorescence (XRF) spectrometry has been used for detecting binding events and measuring binding selectivities between chemicals and receptors. XRF may also be used for estimating the therapeutic index of a chemical, for estimating the binding selectivity of a chemical versus chemical analogs, for measuring post-translational modifications of proteins, and for drug manufacturing.

The invention provides a preparation method and application of monodisperse double-sided structure cyclodextrin-palladium nanosheet/zeolite-like imidazole framework-8 (CD-PdNS/ZIF-8) nano particles. By utilizing two unique large surfaces of two-dimensional palladium nanosheets, utilizing the guidance effect of polyacrylic acid and combining with a selective growth and modification method, a simplemethod for preparing monodisperse double-sided structure CD-PdNS/ZIF-8 nano particles is developed, and the obtained product is good in dispersion, uniform in particle size and good in biocompatibility and has a very broad application prospect in the fields such as dual-drug delivery.