Detection method for obligate hydrocarbon oxidizing bacteria

Abstract

The invention discloses a detection method for obligate hydrocarbon oxidizing bacteria, belonging to the technical field of microorganism detection methods. The detection method comprises the following steps of: suspending and diluting a soil sample with a basic culture medium; performing short-term culturing in combination with a selective culture carbon source and an oxidation reduction indicating dye for 3-5 days; and computing the quantity of obligate hydrocarbon oxidizing bacteria in the soil sample according to the color change of an airtight culture tube through a computer program. A detection technology for obligate hydrocarbon oxidizing bacteria disclosed by the invention has the advantages of simple operating process, economic efficiency, practicability, quickly-obtained result in a short period of time, high specificity, capability of effectively shielding the interference of other bacteria on counting and simple and accurate counting process, and is suitable for large-scale industrial exploration measurement with a large sample quantity.

Description

technical field [0001] The invention relates to the technical field of applied microorganism detection methods, in particular to an obligate hydrocarbon-oxidizing bacteria detection method that provides microorganism detection means for surface microorganism exploration technology. Background technique [0002] Microbial oil and gas exploration is gradually established based on the theory of vertical migration of volatile or semi-volatile hydrocarbons in underground oil and gas reservoirs. With the continuous supply of a certain concentration of hydrocarbons in the surface soil, the microorganisms in the oxidized layer that use hydrocarbons as the sole carbon source can grow and reproduce rapidly, and the difference in abundance can be used to indirectly represent the concentration of light hydrocarbons in the soil. Therefore, the determination of the number of oxidizing microorganisms (that is: obligate hydrocarbon oxidizing bacteria) that use hydrocarbons as the only carbo...

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Problems solved by technology

However, seasonal changes and calcium and magnesium metal ions in the soil will have a greater impact on the final result

[0007] It can be seen that the existing traditional testing techniques and testing methods are far from meeting the requirements of actual production.

Non-specificity, false positives, long incubation time, complexity of experimental operations and equipment, etc. have greatly limited the practical application of obligate hydrocarbon oxidizing microorganism counts

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