23results about How to "Highly toxic" patented technology

The invention discloses a nitrogen fertilizer synergist and application thereof, and belongs to the technical field of fertilizers. The nitrogen fertilizer synergist comprises the following raw materials in percentage by mass: 15-40% of a nitrification inhibitor, 5-10% of butyl dihydrojasmonate, 1-5% of a soil loosening agent, 30-50% of a dispersing aid, 5-10% of an anti-caking agent, 0.1-5% of a trace element fertilizer and 0-5% of nutritional ingredients. According to the nitrogen fertilizer synergist disclosed by the invention, by adding the dihydro jasmonic acid butyl ester, the dispersity of the nitrogen fertilizer synergist can be improved, the fertilizer efficiency of the fertilizer is improved, the growth of plants is promoted, the stress resistance of the plants is improved, the nitrogen fertilizer synergist also has double functions of delaying nitrogen conversion and promoting nitrogen absorption, the utilization efficiency of nitrogen is further improved, and the nitrogen fertilizer synergist is relatively good in flowability and can be applied to the field of agricultural production. The nitrification inhibitor is stable in high-temperature and low-temperature environments, is convenient to use, can be directly used for particle spraying or adding of fertilizer, and solves the problem that nitrification inhibitors such as DMPP can only be used after being dissolved by special solvents.

The invention relates to the technical field of tumor immunotherapy, in particular to a dual-targeting chimeric antigen receptor, a recombinant expression vector, an anti-tumor natural killer cell and application, and provides a PD1-DAP10-41BB-P2A-NKG2D-Ig4-CD28-41BB-CD3 zeta fusion receptor.The dual-targeting chimeric antigen receptor can target PD-L1 on the surface of a tumor cell, can also recognize NKG2DLs and can be used for preparing anti-tumor natural killer cells. The broad spectrum of the NK cells is greatly improved, and meanwhile, the immune escape of tumor cells is also reduced.

The invention discloses an EGFR (epidermal growth factor receptor) dependent micromolecular conjugine-2 as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The invention discloses an EGFR (epidermal growth factor receptor) dependent micromolecular conjugate. The preparation method comprises the following steps: reacting lapatinib with succinic anhydride to obtain an intermediate compound lapatinib-COOH; and then carrying out an amide condensation reaction on the conjugate and an IDO inhibitor NLG919 to obtain the conjugine-2. The result shows that the compound has a strong inhibition effect on pEGFR (Y1068) protein expression in MDA-MB-231 cells of triple negative breast cancer, and the compound can promote differentiation of CD4 + T cells by inhibiting kynurenine in the MDA-MB-231 cells; and the drug resistance of the lapatinib-mediated MDA-MB-231 cell can be effectively reversed.

The invention provides a compound preparation for reducing the toxic effect of zearalenone of bred animals as well as a preparation method and application of the compound preparation, and belongs to the technical field of medicines for livestock and poultry. The invention provides a compound preparation for reducing the toxic effect of zearalenone of bred animals. The compound preparation is prepared from the following components in parts by weight: 40 to 50 parts of total flavonoids of radix astragali seu hedysari, 0.1 to 0.4 part of selenium-enriched yeast, 10 to 15 parts of modified montmorillonite and 1 to 4 parts of vitamin C. The four components in the compound preparation are mutually matched and synergistically synergized to reduce the toxic effect of zearalenone and resist zearalenone poisoning of bred animals, and the compound preparation has high safety, so that the production performance of the bred animals and the quality of animal-derived products are improved, the green and healthy development of animal husbandry is promoted, the preparation method is simple, the cost is low, and the compound preparation is suitable for industrial production. The method is suitable for large-scale production and application.

The utility model relates to a water-soluble hexavalent chromium filtering and collecting device, which belongs to the field of laboratory collection, and comprises a Buchner funnel (1) and a tray (2), the Buchner funnel (1) comprises a funnel opening (3) and a funnel neck (4), the funnel neck (4) is arranged at the bottom of the funnel opening (3), the funnel neck (4) is connected with the tray (2), the Buchner funnel (1) is taken out, and a beaker (11) filled with filtrate is taken out at the same time. The Buchner funnel (1) is provided with a suction filtration bottle (5), the suction filtration bottle (5) is provided with an extraction opening (6), the extraction opening (6) is connected with an extraction pipe (7), the extraction pipe (7) is connected with the suction filtration bottle (5), and the suction filtration bottle (5) is connected with the suction filtration bottle (5). The exhaust pipe (7) is connected with a vacuum pump (8), so that the filtering effect is improved, and the filtering is faster.

The invention belongs to the field of microbial fermentation and the technical field of daily chemicals, and particularly relates to deacetylated sophorolipid as well as a preparation method and application thereof. By using cheap culture medium components and screening out saccharomycetes with high yield of lactone type sophorolipid, the problem that the production cost of the sophorolipid is too high is effectively solved, and the application of the sophorolipid in various fields is promoted; through the step of carrying out enzymolysis on a fermentation substrate in advance, the problems of long single fermentation period, low thallus utilization rate, high energy consumption and high cost of traditional saccharomycetes are solved. A multi-round fermentation circulation system is designed, and bacteria liquid is settled and recycled and then inoculated and fermented, so that thalli are efficiently and repeatedly utilized, the unit yield is increased, the fermentation period is shortened, and the production cost is effectively controlled. The structure is adjusted through a resin modification technology, a final product mainly comprises acetylated sophorolipid, and compared with acetylated sophorolipid and deacetylated sophorolipid, the sophorolipid has better foam stability and sensitive skin repairing effect and is suitable for the application fields of cosmetics, washing care and the like.

The invention discloses a method for detecting nitrofuran metabolites in macrobrachium rosenbergii based on UPLC-MS / MS (Ultra Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) and application of the method, and belongs to the technical field of safety evaluation and detection of aquatic products. According to the method, a macrobrachium rosenbergii sample is pretreated by utilizing a QuECHERS method, nitrofuran metabolites are extracted, and the method comprises the steps of adding acidified acetonitrile for extraction, adding ammonium acetate and sodium chloride for water removal, adding a dispersed solid extraction column for reducing a matrix effect and the like. After the treatment by the QuECHERS method disclosed by the invention, the obtained nitrofuran metabolite does not need to be subjected to a derivatization procedure, and an HILIC hydrophilic chromatographic column can be directly used for carrying out liquid chromatography-mass spectrometry analysis in a positive ion mode so as to detect the nitrofuran metabolite such as semicarbazide and the like. The detection method provided by the invention has the advantages of high sensitivity, strong accuracy, wide applicability and the like, and compared with the traditional detection method, the reagent waste can be avoided, and a large amount of time can be saved.

The invention provides a method for removing antimony by using zirconium functionalized adsorption resin, and belongs to the field of environmental protection. Comprising the following steps: (1) adjusting the pH value of a solution containing antimony ions to 1-11, and then adding zirconium functionalized adsorption resin into the solution to adsorb the antimony ions in the solution containing the antimony ions; and 2) after adsorption is finished, eluting, concentrating, separating and regenerating antimony loaded on the zirconium functionalized adsorption resin by adopting an eluent, so as to realize removal of antimony. According to the method disclosed by the invention, key reaction participants of the method are improved, and the zirconium-functionalized adsorption resin material is applied to antimony recovery, particularly can be applied to adsorption of low-content antimony in mine cavern gushing water, and also can be used for removing arsenic with relatively strong toxicity. The method is easy to operate and suitable for large-scale recovery of low-concentration antimony.

The invention discloses an exosome composition containing resveratrol, astragalus membranaceus and ginseng as well as a preparation method and application of the exosome composition, and belongs to the technical field of biological medicines. The exosome composition disclosed by the invention is obtained by inducing alveolar epithelial cells by active ingredients containing resveratrol, an astragalus extract and a ginseng extract and then secreting the alveolar epithelial cells. The preparation method of the exosome composition mainly comprises the following steps: co-culturing the active component and alveolar epithelial cells, and then sequentially carrying out differential centrifugation and ultracentrifugation on the collected cell supernatant to purify the exosome. The invention also provides a pharmaceutical preparation containing the exosome composition. According to the composition, traditional Chinese medicine active ingredients are combined with a modern cell exosome technology, a potential strategy is provided for prevention and treatment of pulmonary fibrosis, and the composition has a good application prospect.

The invention discloses a cell-based new drug screening method for inhibiting lipid accumulation. The method comprises the following steps: culturing a hepatocyte line, inoculating the hepatocyte line into a culture plate, and adhering the hepatocyte line to the wall for overnight; adding free fatty acid and to-be-detected compounds with different concentrations or an equal amount of solvent into the culture plate for incubation; the method comprises the following steps of: firstly, incubating cells with D-PBS (D-Phosphate Buffer Solution), fixing the cells with paraformaldehyde, washing the cells with D-PBS, incubating the cells with D-PBS containing BODIPY 493 / 503 and DAPI in a dark place, washing the cells with D-PBS, and finally measuring the fluorescence intensity of BODIPY 493 / 503 and the fluorescence intensity of DAPI by using a microplate reader; calculating the ratio of the BODIPY 493 / 503 fluorescence intensity to the DAPI fluorescence intensity, and evaluating the lipid content according to the ratio; and comparing the lipid content of the experimental group with the lipid content of the control group to screen out the drug for inhibiting lipid accumulation. The method is simple and easy to implement, does not depend on expensive instruments and equipment, and can be widely applied to different laboratories and under different conditions.

The invention relates to the technical field of composite nano materials, in particular to a preparation method and application of a ferrocenyl photo-thermal nano material, and the preparation method comprises the following steps: S1, preparing gamma-3-chloropropionyl-L-glutamate which is marked as CPLG; s2, preparing gamma-3-chloropropionyl-L-glutamic acid N-carboxylic acid anhydride, and recording the gamma-3-chloropropionyl-L-glutamic acid N-carboxylic acid anhydride as CPLG S3, preparing methoxy polyethylene glycol-b-poly (gamma-3-azidopropionyl-L-glutamic acid ester), and recording the methoxy polyethylene glycol-b-poly (gamma-3-azidopropionyl-L-glutamic acid ester) as mPEG-b-PAPLG; s4, preparing mPEG-block-poly (gamma-3-ferrocene propionyl-L-glutamic acid ester), and recording the mPEG-block-poly (gamma-3-ferrocene propionyl-L-glutamic acid ester) as MPF ( S5, preparing the ferrocenyl photo-thermal nano material; the prepared ferrocene-based photo-thermal nano material is used for photo-thermal therapy of tumors, an amphiphilic polymer and acetylene ferrocene are used for carrying out a click chemical reaction, the nano material has a nano particle core with higher hydrophobicity, leakage of an organic photosensitizer in the nano material can be effectively prevented, the nano material can cope with a complex in-vivo environment, and the photo-thermal therapy effect is improved.

The invention discloses a glucose oxidase modified gold nanocluster and a preparation method and application thereof, and belongs to the technical field of nano-medicines.The glucose oxidase is added into ultrapure water to be dissolved, then chloroauric acid is added, ascorbic acid is added to assist reduction, and finally the pH is adjusted; reacting under a shading condition, centrifuging, taking supernate, and dialyzing to obtain the glucose oxidase modified gold nanocluster with a molecular formula of AuNCs (at) GOx. The prepared AuNCs GOx has the characteristics of ultra-small size, better dispersity and uniform particle size, and can be applied as a cell autophagy accelerant, an anti-tumor drug and a tumor detection fluorescent agent.

The present invention discloses an EGFR-dependent small molecule conjugate 2 and a preparation method and application thereof, and belongs to the field of biomedical technology. The EGFR-dependent small molecule conjugate disclosed in the present invention is to react lapatinib with succinic anhydride to obtain an intermediate compound lapatinib-COOH; then it is subjected to an amide condensation reaction with the IDO inhibitor NLG919 to obtain conjugate 2. The results show that the compound has a strong inhibitory effect on the expression of pEGFR (Y1068) protein in triple-negative breast cancer MDA-MB-231 cells, and the compound can also promote the differentiation of CD4+T cells by inhibiting kynurenine in MDA-MB-231 cells; it can effectively reverse the drug resistance of MDA-MB-231 cells mediated by lapatinib.

This invention discloses the application of NADK inhibitors in the preparation of drugs for treating liver cancer, such as siRNA targeting NADK. This invention demonstrates that NADK inhibitors can be used to treat liver cancer, and the siRNA provided by this invention exhibits stronger cytotoxicity against HepG2 cells compared to previously disclosed NADK target sequences, effectively inhibiting the proliferation of liver cancer cells both in vitro and in vivo, achieving a tumor-suppressive effect. Furthermore, the siRNA designed in this invention can simultaneously knock down the mRNA levels of both human and mouse NADK genes, facilitating scientific research and the development of tumor drugs.

The invention relates to lactobacillus rhamnosus KXW1 with a hypoglycemic function and application of the lactobacillus rhamnosus KXW1, and belongs to the technical field of microorganisms. In order to excavate a microecological preparation for treating hyperglycemia-related diseases, a strain of lactobacillus rhamnosus KXW1 is separated, and in-vivo and in-vitro experiments prove that the strain has remarkable blood sugar reducing capacity, including promoting proliferation of high-glucose-induced INS-1 cells and increasing the relative expression quantity of MafA genes in the high-glucose-induced INS-1 cells, so that the hyperglycemia-related diseases can be treated. The fasting blood glucose level, postprandial blood glucose level, insulin resistance level, glycosylated hemoglobin level, liver copper ion level and the expression level of copper death related genes FDX1 and DLAT in the liver of the type II diabetes mellitus patient are reduced, and the oral glucose tolerance of the type II diabetes mellitus patient is enhanced. The strain KXW1 provided by the invention can be used for preparing medicines for reducing blood sugar and preventing and / or treating diabetes mellitus or health-care foods for assisting in reducing blood sugar, and opens up a new way for preventing and treating diabetes mellitus.

The application belongs to the technical field of biology, and particularly relates to a bacillus cereus for preventing and treating root-knot nematode and application thereof. The bacillus cereus is named as bacillus cereus ZWL-B-24, is preserved in Guangdong Microbial Culture Collection Center (GDMCC) with an address of 5th floor, building 59, Guangdong Academy of Microbiology, 100, Middle Junli Street, Guangzhou, and a preservation number of GDMCC No: 63680, and a preservation time of July 24, 2023. The bacillus cereus obtained in the application is derived from sweet potato soil, has a strong soil colonization capacity, and due to a long-term coexistence with sweet potato root systems, mutual symbiosis, a series of similar survival mechanisms are formed, and the bacillus cereus is applied to the prevention and treatment of sweet potato root-knot nematode disease, and is more safe and reliable for the growth, edible and processing products of sweet potato.

The application relates to the technical field of anti-counterfeiting materials, in particular to a bio-based anti-counterfeiting filter paper and a preparation method thereof. The application provides a bio-based anti-counterfeiting filter paper which comprises a base material and an anti-counterfeiting dye layer compounded on the base material; the anti-counterfeiting dye layer is prepared from anti-counterfeiting dyes; the anti-counterfeiting dyes are prepared from raw materials including isocyanate, polyethylene glycol, 2,2-dimethylol propionic acid, ethylenediamine and hematoxylin. The bio-based anti-counterfeiting filter paper provided by the application is doped with special anti-counterfeiting dyes, provides unique spectral reflection information, and has the advantages of being difficult to replicate, non-toxic and long service life. Compared with the anti-counterfeiting filter paper currently in circulation, the bio-based anti-counterfeiting filter paper provided by the application can reduce the inflow of fake and inferior products into the market, reduce the use risk of customers, and protect the legal rights and interests of customers.

The present invention discloses a lead-based neutron multiplier suitable for the solid blanket of a fusion reactor, which adopts a composite sphere design with a three-layer core-shell structure: Pb x M y The core material is a lead-based intermetallic compound with a high melting point and excellent neutron multiplication performance, including but not limited to LaPb3, CePb3, Zr5Pb4, Zr5Pb3, PrPb3, NbPb3, Nd5Pb4, Nd5Pb3, PbS, which plays the role of neutron multiplication; the middle shell is Pb x M y ‑Li2O metal-ceramic composite materials, through the efficient production of tritium by Li2O and fusion neutrons to improve the comprehensive tritium breeding ratio of the blanket, while the metal-ceramic composite materials and Pb x M y The metal interface has a good thermodynamic match, achieving a stable overall core-shell structure; Pb x M y The outer shell is a thin film to ensure the chemical inertness of the spherical shell as a whole. The present invention proposes a neutron multiplier design that is economical and can meet the requirements of tritium proliferation in fusion reactors, and can provide a practical solution for replacing pure beryllium.

The invention relates to an RBM-coated CeO2 nano-drug and a preparation method and application thereof.CeO2 nano-particles are wrapped with RBM, then diabetic nephropathy is relieved with the nano RBM drug, and the treatment effect is evaluated; the RBM-CeO2 is passively targeted to the kidney after intravenous injection and releases CeO2 in glomerulus, the RBM-CeO2 enters cells and can inhibit expression of HGMB1 protein, phosphorylation of NF-kB protein is weakened by down-regulation of expression of the HGMB1 protein, glomerular inflammation can be relieved by reduction of phosphorylation of NF-kB, the treatment effect of RBM-CeO2 on relieving of a molecular mechanism of diabetic nephropathy is revealed, and the RBM-CeO2 can be used for treating diabetic nephropathy. The invention aims to provide feasible prevention and treatment measures for patients with diabetic nephropathy.

The invention discloses an attapulgite composite solid waste-based aerogel material as well as a preparation method and application thereof, and the attapulgite composite solid waste-based aerogel material is prepared by taking fly ash, alkaline industrial solid waste and the like as raw materials and attapulgite as a reinforcing material through sol-gel, surface hydrophobic modification and supercritical drying. The density is relatively low, the porosity is relatively high, and the hydrophobic and oleophylic properties are strong. Meanwhile, by doping the attapulgite, not only can the synergistic adsorption performance of the attapulgite and the aerogel be exerted, but also the skeleton strength of the aerogel can be enhanced, so that the cyclic adsorption stability of the aerogel is improved. The adsorbing material also has the advantages of mild and controllable preparation process, low toxicity of the product, environmental friendliness and the like, a new scheme is provided for adsorbing VOCs in waste gas in a complex industrial environment, and high-valued utilization of various bulk solid wastes is realized.

The invention discloses composite paclitaxel albumin nanoparticles as well as a preparation method and application thereof, paclitaxel and a non-nucleotide interferon gene stimulating factor agonist are co-loaded by human serum albumin in a simple manner to form nanoparticles which are uniform in size, proper in particle size, relatively good in dispersity and stable in physicochemical property; the added non-nucleotide interferon gene stimulating factor agonist forms a dimer in a tumor acidic microenvironment, and active targeting of the carrier is combined, so that the nanoparticles have excellent selectivity on tumor tissues; a non-nucleotide interferon gene stimulating factor agonist starts congenital immunity and is combined with adaptive immunity of paclitaxel, so that the defect of low immune responsiveness of'cold 'tumors is overcome. In-vivo and in-vitro toxicity experiments prove that the nanoparticles provided by the invention can solve the problem of strong toxic and side effects of paclitaxel and have synergistic effects on anti-tumor immunotherapy of paclitaxel at the same time, so that the effect of combined treatment is achieved.

The invention belongs to the technical field of fluorescent probes, and particularly relates to a non-oxygen-dependent multi-mode selenium rhodamine photodynamic photosensitizer as well as preparation and application of the non-oxygen-dependent multi-mode selenium rhodamine photodynamic photosensitizer. In order to develop a non-oxygen-dependent and efficient photosensitizer, an N-nitroso functionalized selenium rhodamine photosensitizer SeR-NO is developed on the basis of a selenium rhodamine spiro ring'off-on 'mechanism, and the N-nitroso functionalized selenium rhodamine photosensitizer SeR-NO exists in a ring-closed lactone form in PBS (Phosphate Buffer Solution) and has extremely low cell dark toxicity and excellent cell permeability; under illumination, the photosensitizer not only can generate a large amount of ROS through a type I mechanism and a type II mechanism, but also can catalyze NADPH oxidation and Cyt c reduction in an oxygen-independent manner, so that SeR-NO has high phototoxicity, and can induce cell death in a ferroptosis manner in both normal oxygen (21% O2) and hypoxia (2% O2).