41 results about "Dichocrocis punctiferalis" patented technology

The invention discloses a formula of an artificial feed for dichocrocis punctiferalis, and a manufacturing method of the artificial feed and belongs to the field of artificial feeds for insects. The feed comprises Chinese chestnut powder, maize meal, peach powder, soybean flour, beer yeast powder, multivita-glucose, cholesterol, casein, ascorbic acid, erythromycin, agar powder, methylparaben, methyl parahydroxybenzoats and water. The invention further provides a manufacturing method of the artificial feed for dichocrocis punctiferalis. According to the artificial feed, the Chinese chestnut powder, the maize meal, the soybean flour, the beer yeast powder and the like are added and are in coordination with a plurality of trace elements, so that on the premise of improving the survival rate, the fecundity and the like of dichocrocis punctiferalis, the raising cost is lowered effectively, and the problem that when the conventional artificial feed is used for feeding dichocrocis punctiferalis, larvae refuse feed, the survival rate is low, and the development is not uniform can be solved.

The invention discloses a synthetic Bt insecticidal gene mcry1Ab for transgenosis insect-resistant plants. The Bt insecticidal gene mcry1Ab for transgenosis insect-resistant plants provided by the invention is used for encoding mCry1Ab protein. The mCry1Ab protein is b1), b2) or b3) as follows: b1) the protein with the amino acid sequence as shown in the sequence 2 in a sequence table; b2) the fused protein acquired in the manner of connecting a label with a N end or/and C end of the protein as shown in the sequence 2 in the sequence table; and b3) the protein related to plant insect resistance that is acquired in the manner of substituting and/or deleting and/or adding one or more amino acid residue of the amino acid sequence shown as the sequence 2 in the sequence table. An experiment proves that the mcry1Ab gene can easily acquire the transformant with high expression quantity than the cry1Ab gene, the cry1Ab protein, compared with the mcry1Ab protein, has obvious higher insecticidal activity to ostrinia nubilalis, oriental armyworm, cotton bollworm and dichocrocis punctiferalis, and the mcry1Ab gene has an important popularizing value.

The invention discloses a red illumination method for prompting the mating and oviposition of artificially bred dichocrocis punctiferalis. The method specifically includes the following steps that: temperature in an insectary is controlled in a range of 24 to 26 DEC C, and relative humidity in the insectary is controlled in a range of 40 to 60%, and a common fluorescent lamp is adopted to provide a light source, so that the alternation of day and light can be controlled, and the ratio of the illumination duration of the day to the illumination duration of the light is 15:9; 15% sucrose water is provided for supplementing nutrition for the adults, an apple wrapped with medical gauze is suspended at the top of a cage so as to provide a place for the oviposition of the females; the gauze can be taken off regularly, so that ovums can be obtained; the interior of the insectary is regularly disinfected and is kept ventilating; and in the dark period of a light cycle, a red fluorescent lamp is adopted as a light source, and the fluorescent lamp is suspended right above the cage by 10cm, and the illumination intensity of the four corners of the bottom of the cage is not lower than 2lux assuredly, and illumination time lasts for 9 hours assuredly, namely, red illumination is always provided in the dark period. According to the method, red illumination is provided in the breading period of the adults so as to simulate the natural spectrum at dusk and dawn, and therefore, the dichocrocis punctiferalis can be promoted to mate and oviposit, and breeding effects can be improved. The method is worthy of promotion and use.

The invention relates to a control method for trapping and killing orchard pests by using sweet and sour liquid. In the control method, improved sweet and sour liquid trap devices are utilized, pull line arrangement is conducted between rows of fruit trees, the height of each pull line is 2-2.5 m, and the two ends of each pull line are fixed by using upright rods; the sweet and sour liquid trap devices are hung on the pull lines, and the orchard pests can be trapped, killed and controlled to the greatest extent with the combination of the control method. According to the control method for trapping and killing the orchard pests by using the sweet and sour liquid, compared with a traditional orchard pest control mode by using the sweet and sour liquid, the use and control modes of the sweet and sour liquid are more scientific and efficient, the control method is based on the combination of the life habits of the orchard pests including carposina nipponensis and dichocrocis punctiferalis, the creatively designed sweet and sour liquid trap devices and a scientific and reasonable hanging arrangement mode are utilized, the control effect is greatly improved. The control method is more scientific, more reasonable and more efficient, has good economic values and market values, and is worthy of application and popularization.

The invention relates to the use of insecticidal protein. A method for controlling dichocrocis punctiferalis pests comprises the step of at least contacting the dichocrocis punctiferalis pests with Cry2Ab protein. According to the use of the insecticidal protein, the dichocrocis punctiferalis pests can be controlled by the Cry2Ab protein which is capable of killing the dichocrocis punctiferalis and is generated in plants; compared with the agricultural control method, chemical control method and biological control method which are used in the prior art, the use has the advantages that the insecticidal protein can be used for protecting the whole plants in the whole growth period so as to control the harm of the dichocrocis punctiferalis pests, and is free from pollution and residue, stable and thorough in effect, simple, convenient and economical.

The invention relates to a method for preventing and controlling juicy peach dichocrocis punctiferalis guenee. Microemulsion prepared by regarding plant source extracting solutions as an active ingredient is adopted to perform preventing and controlling, wherein the microemulsion is prepared, by mass percentage, by the following raw materials of 10-20% of the plant source extracting solutions, 15-25% of methyl oleate, 15-20% of styrylphenol polyoxyethylene ether, 3-8% of lauryl polyoxyethylene and the balance water, and the mass percentage sum of the raw materials is 100 percents. The method comprehensively considers the assistant and guide relation among the plant materials, and can obviously improve the prevention and control effect on the juicy peach dichocrocis punctiferalis guenee.

The invention discloses an artificially-synthetic Bt insecticidal gene mcrylF for a transgenic insect-resistant plant. The artificially-synthetic Bt insecticidal gene mcrylF for the transgenic insect-resistant plant, disclosed by the invention, encodes mCry1F protein. The mCry1F protein is b1) or b2) or b3), wherein the b1) is protein with an amino acid sequence shown as a sequence 2 in a sequence table; the b2) is fusion protein obtained by connecting labels at the N end or/and C end of the protein shown as the sequence 2 in the sequence table; the b3) is plant insect resistance-related protein obtained by performing the replacement and/or the loss and/or the addition of one or several amino acid residues on the amino acid sequence shown as the sequence 2 in the sequence table. An experiment shows that compared with a crylF gene, the gene mcrylF more easily obtains a highly-expressed transformant; compared with those of crylF protein, the insecticidal activities of the mcrylF protein for ostrinia nubilalis, mythimna separate, cotton bollworms and dichocrocis punctiferalis are significantly improved; therefore, the popularization value is important.

The invention relates to a method for inhibiting or killing dichocrocis punctiferalis and application thereof, in particular to a method for inhibiting or killing dichocrocis punctiferalis and a method for detecting whether a Cry1Da-7 gene is inserted into a plant genome or not. The method for inhibiting or killing dichocrocis punctiferalis comprises the following steps that (1) a plant of which a genome is inserted into a gene for coding Cry1Da-7 protein as shown in SEQ ID NO: 2 is provided, wherein the plant expresses the Cry1Da-7 protein and generates dichocrocis punctiferalis resistance; and (2) the plant is in contact with dichocrocis punctiferalis, and the dichocrocis punctiferalis is inhibited or killed. It is found that after the Cry1Da-7 gene is inserted into plant genome DNA, the plant shows good dichocrocis punctiferalis resistance, and the insecticidal rate of the Cry1Da-7 gene to dichocrocis punctiferalis reaches 92%-100%.

The invention belongs to the field of chemical synthesis, and particularly relates to an industrial synthesis method of a dichocrocis punctiferalis sex pheromone. The method comprises the following steps: taking 1, 10-decanediol as a raw material; carrying out a single-side bromination reaction to prepare 10-bromodecanol; then reacting the 10-bromodecanol with triphenylphosphine to obtain 10-hydroxydecyltriphenylphosphine salt; performing a Wittig reaction with n-hexaldehyde under the action of alkali to obtain cis-based 10-hexadecene-1-ol; performing isomerization on the cis-based enol underthe action of p-methylthiophenol to obtain trans-based 10-hexadecene-1-ol; and finally performing oxidation under the action of an oxidant to obtain the final product 10-hexadecenal. The method is mild in reaction condition and suitable for large-scale production.

The invention discloses dichocrocis punctiferalis attractive toxicant. The toxicant is prepared from a plant source extracting solution, fruit extracts and additives. The plant source extracting solution is prepared from tea seed cakes, Chinese honey locust, Indian strawberries, Chinaberry seeds, stephania japonica, semiaquilegia adoxoides and radix glycyrrhizae. The fruit extracts are prepared from corn kernels, yellow peaches, Chinese chestnuts and sunflowers. The additives are prepared from white granulated sugar, methyl oleate, rosin and lauryl polyoxyethylene ether sodium sulfate. The dichocrocis punctiferalis attractive toxicant is wide in acting range, and has the same strong attractive killing effect on dichocrocis punctiferalis at different stages, can not do harms to the environment, and is free of obvious changes of the long-term use effect.

A new technique for controlling pomegranate fruit pest Dichocrocis punctiferalis belongs to the field of vegetable disease control. To overcome the defects of the conventional pomegranate fruit pest Dichocrocis punctiferalis control techniques, the new technique includes cleaning a garden; trapping and killing adult Dichocrocis punctiferalis; covering fruits with bags; performing trapping and killing; and performing chemical control so as to control the pomegranate fruit pest Dichocrocis punctiferalis. The new technique is simple to operate, is good in effect, and can effectively decrease the incidence rate of the pomegranate fruit pest Dichocrocis punctiferalis.

The invention aims at the damage caused by five types of pests including Cryptothelea variegate Ellen, cnidocampa flavescens, zeuzera coffeae Nietner, scale insects and dichocrocis punctiferalis often existing on pomegranate trees. By adopting an artificial prevention and control and pesticide-spraying prevention and control combined mode, disease and pest damage are effectively prevented and controlled, the pesticide application amount is decreased to the greatest extent, and the pomegranate yield is increased.

The invention discloses a determination method and application of a volatile active compound having an effect of attracting dichocrocis punctiferalis. The method comprises the following steps: Step 1,determining a trapping source: taking an apple inoculated with penicilliums as the trapping source; Step 2, trapping volatile matters: performing headspace trapping and elution on the volatile matters of the apple inoculated with the penicilliums; Step 3, identifying the volatile matters: identifying the volatile matters trapped by a gas chromatograph-mass spectrometer, so as to determine a compound required to be screened; Step 4, determining an EAG (electroantennogram) electrophysiological response, so as to acquire determination data; Step 5, determining a Y type olfactometer behavior response and calculating a selection percentage; Step 6, analyzing the acquired data, so as to determine that the volatile active compound having the strongest effect of attracting the dichocrocis punctiferalis is isoamyl alcohol. According to the method, the volatile active compound having the better effect of attracting the dichocrocis punctiferalis can be accurately determined, so as to provide anaccurate evidence for producing a dichocrocis punctiferalis attractant having the better attractive effect and provide a powerful guarantee for management of dichocrocis punctiferalis pests.

The invention discloses microorganism organic fertilizer special for corn. The microorganism organic fertilizer is prepared from the following raw materials in parts by weight: 13 to 15 parts of castor cakes, 9 to 11 parts of wormcast, 2 to 4 parts of duck egg shell, 3 to 5 parts of cottonseed hull, 2.4 to 2.6 parts of scarp, 1.6 to 1.8 parts of gingko biloba testa, 3.1 to 3.3 parts of pepper leaves, 3.4 to 3.6 parts of bamboo leaves, 4.1 to 4.3 parts of distillers grains, 0.6 to 0.8 part of algin, 0.6 to 0.8 parts of ox horn, 0.3 to 0.4 part of compound microorganism bacteria, 1.3 to 1.5 parts of bone oil, 2.4 to 2.6 parts of swill, 3.2 to 3.4 parts of humus and 2.1 to 2.3 parts of dolomite. The microorganism organic fertilizer disclosed by the invention is scientific and reasonable in formula, effective nutrient components of all raw materials are in mutual integration and have the functions of supplementing each other, the physical property and the biological activity of soil can be improved, and high ripening rate and full grains are obtained through enhancing the absorption and utilization of the corn on the nutrient components; bacterial wilt and dichocrocis punctiferalis of the corn can be effectively prevented, so that the yield of the corn is increased, and higher economic profit is brought for farmers.

The invention discloses a method for feeding dichocrocis punctiferalis on a large scale, and belongs to the field of insect feeding. The method comprises the following four steps: collection and incubation of dichocrocis punctiferalis eggs, feeding of larvas at the age of 1-3 of the dichocrocis punctiferalis, pupation of larvas at the age of 3 to aged larvas, and feeding of adult dichocrocis punctiferalis. Spawning and collection of the dichocrocis punctiferalis are solved by degreasing cotton, spawning capacity of single female dichocrocis punctiferalis is high, and the hatching rate is high;and secondarily, by the method, newly hatched larvas are fed with tender corns, requirements for nutrition of the larvas at the age of 3 are met, meanwhile, escaping of the larvas can be prevented, and a large number of larvas at the age of 3 can be provided for field inoculation tests; and moreover, compositions of an artificial feed in the method are simple, materials are easily obtained, the cost is low, requirements for nutrition of aged larvas can be met, pollution can be avoided easily, feed change frequency is reduced, and the labor and time are saved. By the artificial feed, large-scale artificial subculture feeding of the dichocrocis punctiferalis can be carried out. The method has the advantages of high feeding efficiency, low feeding cost, high survival rate of the dichocrocispunctiferalis and the like.

The invention discloses a technology for preventing and controlling the pest dichocrocis punctiferalis of pomegranate fruits, and belongs to the field of disease prevention and control of fruits and vegetables. According to the technology provided by the invention, in order to solve the technologic defect for preventing and controlling the dichocrocis punctiferalis in the prior art, the followingmethods are adopted: cleaning a garden, trapping and killing adult pests, carrying out fruit bagging, carrying out trapping and killing, and carrying out prevention and control with reagents, so thatthe purpose of preventing and controlling the pest dichocrocis punctiferalis of the pomegranate fruits is achieved.

The invention provides a reagent for controlling dichocrocis punctiferalis of a red peach and a using method of the reagent, and belongs to the technical field of agricultural planting. The reagent selects a plant extract as a main pesticide and is cooperated with alum, plaster, lime and the like, so that the reagent has not only the effects of preventing and killing the dichocrocis punctiferalis, but also a high degradation rate and does not pollute fruits.

The invention discloses a comprehensive control method for dichocrocis punctiferalis in an orchard for hylocereus undatus Foo-Lon. The control method involves physical control, chemical control and biological control to achieve control over dichocrocis punctiferalis in the orchard for hylocereus undatus Foo-Lon. According to the chemical control, after the orchard is cleaned in winter every year,firstly, an overwintering insecticide is scattered in the orchard, and then overwintering fertilizer is applied; in March-April every year, in combination with the application of spring fertilizer, apest-prevention agent is scattered in the orchard; from June every year, adult pests of dichocrocis punctiferalis enter an oviposition high-peak period and an egg incubation high-tide period, monitoring and forecasting are conducted in time, when the adult pests of dichocrocis punctiferalis are found, dichocrocis punctiferalis insecticide is continuously sprayed twice in time, the time interval ofthe two times of insecticide spraying is one week, and the insecticide is used up within 3-4 days every time. By means of the method, dichocrocis punctiferalis can be controlled, and the damage of dichocrocis punctiferalis is greatly reduced; moreover, a certain control effect is achieved on other diseases and pests, the incidence of the diseases and pests in the orchard is reduced, the yield andsingle-fruit weight of the dragon fruits can be increased, and the commercial fruit rate can be increased to 91% or above.

A novel technology for controlling pomegranate fruit pest Dichocrocis punctiferalis is disclosed. In order to solve deficiencies in current control technology of pomegranate fruit pest Dichocrocis punctiferalis, the novel technology employs means of clearing gardens, trapping and killing adult pests, bagging fruits, conducting trapping and killing, and using pesticides for control, so that controlling on pomegranate fruit pest Dichocrocis punctiferalis is achieved. The novel technology is simple to operate, good in effect and free of additional cost input, and can effectively reduce incidenceof the disease.

The invention discloses a determination method and application of a volatile active compound with a repelling effect on dichocrocis punctiferalis, and the method comprises the following steps: step 1,respectively collecting sunflower disc volatile matters in a flowering period and a mature period by using a headspace dynamic gas sampling method, and analyzing the collected volatile matters by using a gas chromatograph-mass spectrometer, respectively identifying the types of sunflower disc volatiles in two periods; 2, comparing and analyzing sunflower disc volatile matters in a flowering period and a mature period, and selecting 12 volatile compounds which are respectively unique in the two periods and have large content change between the two periods to carry out a dichocrocis punctiferalis mated female moth behavior reaction test; and 3, testing the behavior reaction of the mated female moths of the dichocrocis punctiferalis to the selected 12 compounds by using a Y-shaped olfactometer, and determining that the [beta]-phellandrene can be used as a repellent of the dichocrocis punctiferalis when the concentration is 0.01 (v/V). According to the method, the volatile active compoundwith the repelling effect on the dichocrocis punctiferalis is accurately determined.

The invention relates to an insecticidal composition containing bucloveramide and application thereof. The insecticidal composition comprises an active ingredient A and an active ingredient B, whereinthe active ingredient A is bucloveramide and the active ingredient B is one of abamectin, methylamino abamectin benzoate and ivermectin. The weight ratio of the active ingredient A and the active ingredient B is (10-1): (1-10). The composition can be applied to preparing pesticides into various dosage forms permitted agriculturally. The compound components are an amide novel broad spectrum pesticide and an antibiotic pesticide, so that the composition has a relatively good effect in preventing and treating rice leaf folder, plutella xylostella, european corn borer, dichocrocis punctiferalis,prodenia litura and cotton bollworm of various economical crops such as rice, vegetables, corn and citrus, and can effectively delay generation of resistance of injurious insects. Compared with a single preparation, the composition is good in control effect and long in lasting period, expends the insecticidal spectrum and is obvious in synergism.

The invention belongs to the technical field of pesticides and particularly relates to an insecticide composition comprising butyl chlorantraniliprole and nicotine. The insecticide composition comprises an effective ingredient A and an effective ingredient B, wherein the effective ingredient A is butyl chlorantraniliprole, and the effective ingredient B is one of imidacloprid, acetamiprid and nitenpyram. The weight ratio of the effective ingredient A to the effective ingredient B is (10-1):(1-10). The provided composition can be prepared into various agriculturally allowable preparations, hasbetter effects on preventing and controlling rice leaf folders, plutella xylostella, european corn borer, dichocrocis punctiferalis, prodenia litura, cotton bollworms, aphids, thrips and leafhoppers of various commercial crops like rice, vegetables, corns and citrus, and can effectively delay the production of pest resistance; compared with the single preparation, the prevention and control effects are good, the lasting period is long, the insecticidal spectrum is expanded, and the synergistic effect is obvious.