Method for controlling pests

A kind of pest, peach borer technology, applied in the field of pest control

Active Publication Date: 2013-03-20
BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] It has been proved that the plants transgenic for Cry1Fa can resist the attack of Lepidoptera pests such as the small cutworm. However, there is no report on the control of peach borer damage to plants by producing transgenic plants expressing Cry1F protein.

Method used

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  • Method for controlling pests
  • Method for controlling pests
  • Method for controlling pests

Examples

Experimental program
Comparison scheme
Effect test

no. 1 example

[0085] The first embodiment, the acquisition and synthesis of Cry1Fa gene

[0086] 1. Obtain the Cry1Fa nucleotide sequence

[0087] The amino acid sequence (605 amino acids) of the Cry1Fa-01 insecticidal protein, as shown in SEQ ID NO: 1 in the sequence listing; Cry1Fa-01 encoding the amino acid sequence (605 amino acids) corresponding to the Cry1Fa-01 insecticidal protein Nucleotide sequence (1818 nucleotides), as shown in SEQ ID NO:3 in the sequence listing; amino acid sequence (1148 amino acids) of Cry1Fa-02 insecticidal protein, as shown in SEQ ID NO:2 in the sequence listing ; Cry1Fa-02 nucleotide sequence (3447 nucleotides) encoding the amino acid sequence (1148 amino acids) corresponding to the Cry1Fa-02 insecticidal protein, as shown in SEQ ID NO:4 in the sequence listing.

[0088] 2. Obtain the nucleotide sequences of Cry1Ab and Vip3A

[0089] The Cry1Ab nucleotide sequence (1848 nucleotides) encoding the amino acid sequence (615 amino acids) of the Cry1Ab insectic...

no. 2 example

[0092] The second embodiment, construction of recombinant expression vector and transformation of recombinant expression vector into Agrobacterium

[0093] 1. Construction of a recombinant cloning vector containing the Cry1F gene

[0094] The synthesized Cry1Fa-01 nucleotide sequence was connected to the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and the operation steps were carried out according to the instructions of the pGEM-T vector produced by Promega Company to obtain the recombinant cloning vector DBN01-T , its construction process is as follows figure 1 Shown (wherein, Amp represents the ampicillin resistance gene; f1 represents the replication origin of phage f1; LacZ is the LacZ start codon; SP6 is the SP6 RNA polymerase promoter; T7 is the T7 RNA polymerase promoter; Cry1Fa-01 is the Cry1Fa-01 nucleotide sequence (SEQ ID NO: 3); MCS is the multiple cloning site).

[0095]Then, the recombinant cloning vector DBN01-T was transformed into Escherichia ...

no. 3 example

[0107] The third embodiment, the acquisition and verification of corn plants transferred to the Cry1F gene

[0108] 1. Obtaining corn plants transferred to the Cry1F gene

[0109] According to the commonly used Agrobacterium infection method, the immature embryos of the aseptically cultured maize variety Zong 31 (Z31) were co-cultured with the Agrobacterium described in 3 in the second example, so that the T-DNA in recombinant expression vectors DBN100014, DBN100012 and DBN100276 (including the promoter sequence of corn Ubiquitin gene, Cry1Fa-01 nucleotide sequence, Cry1Fa-02 nucleotide sequence, Cry1Ab nucleotide sequence, Vip3A nucleotide sequence , PMI gene and Nos terminator sequence) were transferred into the maize genome, and the maize plants transferred to the Cry1Fa-01 nucleotide sequence, the maize plants transferred to the Cry1Fa-01-Cry1Ab nucleotide sequence and the transferred Cry1Fa - the maize plant of 02-Vip3A nucleotide sequence; meanwhile, the wild type maize...

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Abstract

The invention relates to a method for controlling dichocrocis punctiferalis, which comprises the steps of getting the dichocrocis punctiferalis to be contacted with protein of CrylF. According to the method, the dichocrocis punctiferalis is controlled through the protein of CrylF which is capable of killing the dichocrocis punctiferalis and is generated in a plant. Compared with the prior art of agricultural control method, chemical control method and biological control method, the method can be used for protecting the whole plant from being damaged by the dichocrocis punctiferalis in the whole growth period, and the method has the advantages of being pollution-free, stable in effect, thorough in killing pests, simple, convenient and economic, and having no residue.

Description

technical field [0001] The invention relates to a method for controlling pests, in particular to a method for using Cry1F protein expressed in plants to control damage to plants by the peach borer borer. Background technique [0002] Peach borer (Conogethes punctiferalis) belongs to the family Lepidoptera and is an omnivorous pest. It not only damages crops such as corn and sorghum, but also damages fruit trees such as peaches, persimmons, and chestnuts. It is widely distributed in my country, starting from Heilongjiang and Inner Mongolia in the north. , south to Taiwan, Hainan, Guangdong, Guangxi, and the southern edge of Yunnan, east to the former Soviet Union, North Korea, west from Shanxi, Shaanxi to Ningxia, Gansu, and then to Sichuan, Yunnan, and Tibet. When maize is damaged, it mainly eats the ears and stems, and the plant damage rate reaches 30%-80%; when it damages sorghum, the newly hatched larvae eat into the young sorghum grains, and seal the mouth with feces or f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N47/44A01P7/04A01N63/50
CPCC07K14/325C12N15/8286A01N63/02A01N37/46C12R2001/07C12N1/205Y02A40/146A01N63/50A01N63/23
Inventor 康越景张爱红庞洁王登元刘海利黄金存魏雪松李春乐田康乐宋庆芳
Owner BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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