534 results about "Sorghum" patented technology

The invention discloses a SbWRKY51 gene, a promoter and application of the SbWRKY51 gene and the promoter in improvement of salt tolerance of sorghum, and belongs to the technical field of plant genetic engineering. The SbWRKY51 gene is screened from sorghum, and the expression level of the SbWRKY51 gene is up-regulated after salt stress treatment. The protein coded by the gene belongs to a WRKY transcription factor family, has transcriptional activation activity, and can start the expression of a reporter gene. Sorghum overexpression strains and complementary strains of the gene are obtained, salt tolerance related physiological indexes are measured, overexpression of the gene can improve the seed germination rate, increase the seed root length, regulate ion balance and enhance the free radical scavenging capacity of plants, and the effect of the gene in the aspect of improving the salt tolerance is embodied. A further research finds that the SbWRKY51 gene can enhance the plant salt tolerance by regulating a lignin synthesis pathway. The technical scheme provides a basis for cultivating resistant plants, and has important guiding significance for enhancing the production potential of sorghum under a high-salt condition and promoting agricultural development.

The invention belongs to the technical field of biotechnology and agricultural biological breeding, and relates to a sorghum double haploid breeding method which comprises the following steps: (1) performing gene mutation on SbMTL and SbDMP to obtain a sorghum double-gene mutant strain according to any one of claims 1 to 3; (2) screening and identifying sorghum haploids by using GFP (Green Fluorescent Protein) marker and Ruby marker genes; and (3) naturally doubling the obtained haploid plant to obtain the double haploid of sorghum. SbMTL and SbDMP genes of sorghum are knocked out by utilizing a CRISPR / Cas9 technology so as to realize high-frequency haploid induction, meanwhile, an effective haploid selection marker is introduced, the induction frequency of a haploid induction system is evaluated, and after the obtained haploid plant is naturally doubled, a double-haploid system of sorghum is successfully obtained. The induction efficiency of the sorghum haploid induction line reaches up to 18% or above, the economic value is high, and the process of sorghum breeding is remarkably promoted.

The invention discloses a sorghum dwarf gene PCR (Polymerase Chain Reaction) detection primer and application thereof, and belongs to the technical field of biology. The specific PCR primer is designed and compared with a wild sorghum sequence after being subjected to PCR amplification, so that whether a germplasm resource contains the dwarf gene or not can be determined, the mutation site and the mutation type of the dwarf gene can be determined, and a foundation is laid for quickly screening dwarf sorghum varieties, performing cross breeding in the next step and the like.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a gene SbAIF1 for enhancing sorghum alkali tolerance and application of the gene SbAIF1. According to the invention, the gene SbAIF1 is separated and cloned from sorghum, the CDS sequence of the SbAIF1 gene is connected with pCAMBIA2300-GFP-BWM, and the sorghum is transformed by using an agrobacterium-mediated method. Experimental results show that the SbAIF1 gene is over-expressed in sorghum, the alkali tolerance of the sorghum is enhanced after the gene is over-expressed, meanwhile, the survival rate of the sorghum under alkali stress can be increased after the gene is over-expressed, and a new thought is provided for cultivating saline-alkaline tolerant transgenic plants.

The invention discloses a special bacterial fertilizer for sorghum and application of the special bacterial fertilizer in prevention and control of sorghum diseases, and belongs to the technical field of microbial fertilizers and agriculture. The special bacterial fertilizer for sorghum comprises the following components in parts by weight: 8-10 parts of a composite microbial agent, 40-50 parts of sorghum residues, 5-10 parts of bentonite, 1-2 parts of potassium humate, 1-2 parts of composite trace elements, 10-20 parts of sawdust and 10-20 parts of rice bran, wherein the complex microbial inoculant consists of a bacillus subtilis microbial inoculant, a rhizobium microbial inoculant, a bacillus velezensis microbial inoculant and a trichoderma harzianum microbial inoculant. The special bacterial fertilizer for sorghum provided by the invention contains rich microorganisms and nutrient substances, and can effectively prevent and control sorghum diseases and enhance the disease resistance of sorghum. The components such as microorganisms and organic acids in the special bacterial fertilizer for sorghum can promote the formation of soil organic matters, improve the soil structure, improve the soil quality and effectively improve the growth environment of sorghum.

The invention discloses a sorghum aphid MsCYP1 gene as well as an encoding protein, dsRNA (double-stranded ribonucleic acid) and application thereof, and aims to solve the technical problem that a key gene is lacked when an RNA (ribonucleic acid) interference technology is used for biological prevention and control of sorghum aphids at present. According to the application, the sorghum aphid protein MsCYP1 capable of being secreted into sorghum cells is screened, and the protein and the coding gene thereof can inhibit the content of hydrogen peroxide in sorghum and the immune response of the sorghum. Meanwhile, according to the cDNA sequence of the MsCYP1 gene of the sorghum aphid, dsRNA for the gene is designed, the dsRNA is introduced into the body of the sorghum aphid in an artificial feeding mode, it is found that the dsRNA can remarkably inhibit the MsCYP1 gene of the sorghum aphid, then the honeydew secretion amount and the reproduction rate of the sorghum aphid are affected, the MsCYP1 gene can serve as an RNAi target gene to be used for aphid prevention and control, pesticide use is reduced, and the application prospect is good. The ecological balance is maintained.

The invention provides a moniliella malayi and a fungicide and application thereof, and relates to the field of microorganisms. The acer rubrum is specifically acer rubrum RA, and the preservation number of the acer rubrum RA is CGMCC (China General Microbiological Culture Collection Center) No.35889. The acer rubrum RA can be used for preparing the acer rubrum. The strain has the capability of decomposing inorganic phosphorus, is wide in phosphorus-dissolving range, can decompose tricalcium phosphate, iron phosphate and aluminum phosphate, and particularly still has very strong capability of decomposing inorganic phosphorus under a low-temperature condition; the strain has the capability of producing siderophore and IAA at low temperature; the strain has an inhibition effect on various pathogenic bacteria causing field crop diseases, and can inhibit diseases such as corn root rot, sorghum leaf spot or wheat root rot; the strain can be colonized in a soil environment of 5-40 DEG C, a microbial inoculum prepared from the strain can promote crop growth, the crop yield is remarkably improved, and high yield can be achieved even if the usage amount of phosphate fertilizer is reduced.

The invention provides a cronobacter malonate strain and application thereof, the strain is preserved in China Center for Type Culture Collection on January 15, 2025, the preservation date is January 15, 2025, and the preservation number is CCTCC M 2025146. The strain has excellent capability of producing 4-vinyl guaiacol, and the yield in a sorghum juice fermentation culture medium is 2.70 + / -0.15 mg / L; meanwhile, the strain can resist ferulic acid and has good capability of converting ferulic acid to generate 4-vinyl guaiacol, and when 0.1% (w / w) of ferulic acid is added into a fermentation culture medium, the content of 4-vinyl guaiacol reaches 2.68 + / -0.04 g / L. The strain is applied to production of Maotai-flavor liquor, the content of 4-ethyl guaiacol in raw liquor reaches 3.20 + / -0.26 mg / L and is remarkably increased by 223.23% (P is smaller than 0.01) compared with the content of 4-ethyl guaiacol without the strain, the Maotai flavor is prominent through sensory evaluation, the flower, fruit and burnt flavor is obvious, and the strain has important significance on improvement of the health quality of the liquor.

The invention relates to a composite microbial agent suitable for saline-alkali soil remediation of sorghum production in northeast alpine regions, and a preparation method and application thereof. The composite microbial agent comprises bacillus subtilis, bacillus mucilaginosus and pseudomonas fluorescens. Bacillus mucilaginosus capable of efficiently producing polyglutamic acid and potassium and pseudomonas fluorescens capable of secreting chitinase are separated and screened from obstacle soil in northeast alpine regions, and field trials are carried out in the obstacle soil (coastal saline-alkali soil) in the northeast alpine regions. The low-temperature slow-release fertilizer has the unique characteristics of secreting polyglutamic acid, potassium and chitinase under the low-temperature condition, is low in cost, and can effectively improve the physical and chemical properties of soil, increase the seed emergence rate, stimulate the root growth and enhance the salt resistance and alkali resistance of seedlings after being applied.

The invention provides grain sorghum combine harvesting equipment, and relates to the technical field of grain harvesting, the grain sorghum combine harvesting equipment comprises a vehicle body and a harvesting bucket, the top end of the harvesting bucket is fixedly connected with a connecting frame, one side of an inner cavity of the connecting frame is rotatably connected with a rotating rod, and the outer surface of the rotating rod is fixedly connected with a reel for pulling an object to be cut; a driving assembly is arranged at the end, close to the vehicle body, of the harvesting hopper, a cutting knife assembly used for grain harvesting is fixedly installed on one side of an inner cavity of the harvesting hopper, through grooves are formed in the two sides of the end, close to the vehicle frame, of the harvesting hopper, and the cutting knife assembly comprises a cutting table fixedly connected to one side of the inner cavity of the harvesting hopper; top blocks are fixedly connected to the two sides of the top end of the guide plate, a scraping plate used for removing blockages is arranged at the top end of the guide plate, crops blocked in the blades are cleaned through the scraping plate, the cutting effect of the cutter assembly is guaranteed, meanwhile, manual cleaning is not needed, and the working efficiency of the equipment is effectively improved.

The present invention relates to the technical field of agricultural fertilizers, and discloses a biofertilizer synthesized from Chinese herbal medicines, a preparation method and an application thereof, wherein the biofertilizer is prepared by combining herbal extracts such as astragalus, dandelion, forsythia, gallnut, and liquorice with Chinese herbal medicine residues such as honeysuckle residue, tobacco residue, malt residue, yeast residue, sophora flavescens residue, coptis chinensis residue, and stemonae residue, and is combined with organic fertilizers and nitrogen, phosphorus, and potassium fertilizers, and is fermented by composite microorganisms. The obtained biofertilizer is not only comprehensive in nutrients and easy to absorb, but also rich in natural antibacterial, anthelmintic substances and active microorganisms, which helps to promote crop growth and enhance disease and insect resistance. At the same time, long-term use can improve soil structure, enhance soil fertility and microecological balance, and reduce the use of chemical fertilizers and pesticides, thereby achieving the effects of increased production and efficiency, green environmental protection, and sustainable development.

The invention discloses a strain of Pseudomonas sp., which is named as Pseudomonas sp. CY58, is preserved in China Center for Culture Collection on September 27, 2024, and has a preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20242104. The invention also discloses a method for preparing the Pseudomonas sp. The invention also discloses a microbial agent containing the Pseudomonas sp. CY58 as well as a preparation method and application of the microbial agent. The microbial agent containing the Pseudomonas sp. CY58 or the fermentation broth thereof disclosed by the invention can effectively prevent and treat tea anthracnose, pepper anthracnose, apple anthracnose and sorghum anthracnose, obviously slows down the plant disease condition, has a good prevention and treatment effect, especially has the best prevention and treatment effect on pepper anthracnose, and has a good application prospect. A new direction is found for preventing and treating the plant anthracnose.

The invention relates to the technical field of saline-alkali soil improvement, and particularly discloses a method for improving light and moderate saline-alkali soil through winter rye crop rotation, which comprises the following steps of land parcel preparation, winter rye sowing, winter rye field management, management after winter green turning, winter rye harvesting, feeding sweet sorghum sowing and feeding sweet sorghum field management. According to the method, the moderate saline-alkali soil is improved by sowing the winter rye and sowing the feeding sweet sorghum, and after crop rotation of the winter rye and the feeding sorghum, the salt content and pH of the soil are effectively reduced, the soil structure is improved, and the saline-alkali soil is suitable for ploughing. Meanwhile, high economic benefits can be brought, two seasons of forage grass income is obtained per mu, meanwhile, the sediment transport amount in spring is obviously reduced in a land parcel covered with winter rye, compared with a corn straw stubble field, the sediment transport amount in the same period of the rye field is reduced, and losses of organic matter and nutrients of surface soil are reduced.

The invention discloses Klebsiella and application thereof, and belongs to the technical field of microorganisms. According to the invention, firstly, a strain LLMJ-DNXJ-01 with high yield of tannase is screened from wine-making yeast, the identification result shows that the strain belongs to klebsiella, then the tannase activity of the strain is investigated, and the result shows that the enzyme activity reaches 8.8 + / -0.02 U / mL. In addition, the invention also investigates the application of the strain in degrading sorghum tannin, the sorghum tannin degradation rate reaches 34.95%, and isoamyl alcohol, isopentenol, caryophyllene, phenethyl alcohol, 4-vinyl guaiacol and other volatile aroma substances with Daqu characteristics can be generated through fermentation. Therefore, by integrating the tannase production capability of the strain LLMJ-DNXJ-01 and the sorghum tannin degradation and fermentation aroma production capability, the strain has relatively good development potential in the aspects of manufacturing of reinforced yeast for making hard liquor and quality improvement and aroma enhancement of white spirit.

The invention relates to the technical field of soil improvement, in particular to a method for suppressing saline-alkali soil in autumn and winter and ploughing in early spring, improving soil moisture and controlling salt in saline-alkali soil, which utilizes the fact that a large amount of moisture migrates from deep soil or underground water to the surface layer in the winter soil freezing process of a water-deficient saline-alkali area and the moisture content of the surface layer soil is obviously increased after the soil freezing layer is ablated in spring. According to the dynamic law of soil water and salt in the soil freezing and thawing process, a compacting machine is used for compacting the land before soil freezing in winter in the current year, the compactness of the soil is improved, the soil pore proportion of rising water is increased, the soil freezing process in winter is used for promoting the migration of deep soil moisture to the surface layer, and in the early spring of the next year and in the soil freezing layer ablation and slurry returning period, the soil moisture content is increased. A large-scale ploughing machine is adopted to conduct deep ploughing on the land, the conditions of high moisture and low salinity of a plough layer are kept, and normal seedling emergence and growth of spring crops are guaranteed in combination with micro-ditch sowing of spring sowing crops such as sorghum, oil sunflowers and oats.

The invention discloses a sorghum brown midrib mutant gene bmr34 related to high digestibility and application of the sorghum brown midrib mutant gene bmr34. The haplotype of the mutant gene bmr34 can specifically reduce the lignin content by 2.99% without affecting the biological yield. The invention provides the KASP marker which can accurately locate the bmr34 gene of sorghum and is closely linked with the bmr34 gene of sorghum, so that the breeding efficiency can be greatly improved, the breeding process is accelerated, and the KASP marker has important theoretical and practical significance for improving the quality of sorghum feed.

The invention discloses a biochar-reinforced hydrogel slow-release fertilizer and a preparation method thereof, and relates to the technical field of fertilizers. Comprising the following steps: S1, preparing sorghum straw biochar; s2, adding the sorghum straw biochar and a potassium nitrate and ammonium dihydrogen phosphate solution into a polymer monomer mixed solution to obtain a biochar gel mixed solution; s3, adding the genipin solution into the biochar gel mixed solution, stirring, performing ultrasonic treatment, freezing and unfreezing to obtain the biochar reinforced hydrogel slow-release fertilizer. Aiming at the technical defects of the hydrogel slow-release fertilizer, the performance of the hydrogel slow-release fertilizer is modified and enhanced by utilizing the biochar, and the biochar-enhanced hydrogel slow-release fertilizer is formed. The preparation method is simple in process and convenient to operate, the raw materials are cheap and easy to obtain, the cost is low, and the prepared biochar reinforced hydrogel slow-release fertilizer has the advantages of good slow-release performance, high water retention capacity and environmental stability, and is of great significance in popularization and application of the hydrogel slow-release fertilizer.

The invention relates to the technical field of food engineering, and discloses pediococcus acidilactici, a microbial agent containing the pediococcus acidilactici, application of the microbial agent, sorghum fermented food and a preparation method of the sorghum fermented food. The preservation number of the pediococcus acidilactici is GDMCC (China General Microbiological Culture Collection Center) No.65676, and the pediococcus acidilactici can be used for preparing the pediococcus acidilactici. The microbial agent contains pediococcus acidilactici and auxiliary bacteria. According to the sorghum fermented food obtained through fermentation treatment of the fungicide, antinutritional factors such as tannin in sorghum can be converted into urolithin, hydrolysis of protein, fat and starch can be promoted, the utilization rate of the sorghum is increased, the quality and flavor of fermented products are improved, the fermentation period is shortened, the shelf life is prolonged, and high economic benefits are achieved.

The invention relates to the technical field of preparation processes, and discloses ancient Huangtai liquor, which is prepared from the following components in percentage by weight: 50 to 55 percent of base liquor, 10 to 15 percent of medicinal and edible traditional Chinese medicinal material extract, 2 to 4 percent of honey, 0.1 to 2 percent of flavor regulator and the balance of purified water, the medicinal and edible traditional Chinese medicinal materials comprise at least one of angelica sinensis, lily, radix ophiopogonis, Chinese dates, Chinese wolfberry fruits, American ginseng, rhizoma polygonati, papayas, astragalus membranaceus and poria cocos, and the liqueur is prepared through the processes of stepped extraction, compound blending, ultrasonic-assisted homogenization, dynamic ageing and membrane filtration. According to the invention, through a stepped extraction process, temperature and time are regulated and controlled in stages, volatile components are preferentially extracted at low temperature, and then non-volatile substances are extracted at medium temperature, so that effective components in the traditional Chinese medicinal materials are fully extracted, the natural flavor of the traditional Chinese medicinal materials is retained, the extraction rate of the effective components is improved, and the flavor level of the liqueur is enriched.

The invention provides a gene SbERF71 capable of improving waterlogging tolerance of sorghum, protein, application and a breeding method, and belongs to the technical field of crop breeding. On the basis of a sorghum plant with waterlogging tolerance, it is determined that SbERF71 is a key gene affecting the waterlogging tolerance of sorghum through RNA extraction and library building, RNA-Seg data analysis, weighted gene co-expression network analysis (WGCNA) and other methods. The SbERF71 gene is related to gene expression of various stress reaction pathways (such as energy metabolism and aero-tissue formation) in sorghum, is a central regulatory factor of sorghum waterlogging, and is beneficial to reaction of sorghum to flooding stress, expression height up-regulation of sorghum in a flooding stress environment and enhancement of flooding resistance of sorghum, so that sorghum can adapt to waterlogging stress. The invention reveals that sorghum can adapt to molecular and physiological mechanisms of waterlogging stress, provides a new direction for breeding of sorghum waterlogging-resistant varieties, and makes a contribution to food safety in waterlogging agricultural areas.

The invention discloses a sorghum anthracnose susceptible gene SbABCA7, a recombinant plasmid and application of the sorghum anthracnose susceptible gene SbABCA7 and the recombinant plasmid. The sorghum anthracnose susceptible gene SbABCA7 is a sorghum susceptible gene, the nucleotide sequence of the sorghum susceptible gene SbABCA7 is SEQ ID NO.01, and the amino acid sequence of the SbABCA7 protein gene is SEQ ID NO.02. The invention further discloses a preparation method of the sorghum anthracnose susceptible gene SbABCA7. The susceptible gene SbABCA7 of the sorghum anthracnose can be subjected to amplification, recovery, plasmid recombination and homologous genetic transformation to obtain a positive sorghum plant; a primer designed by the susceptible gene SbABCA7 can be directly used as a molecular marker to quickly screen and identify sorghum anthracnose-resistant resources in a seedling stage; the sorghum susceptible gene can be mutated into a disease-resistant gene through a gene locus, and the SbABCA7 sorghum susceptible gene can be changed into the disease-resistant gene through technologies such as gene editing; the invention can provide a convenient method for rapidly screening materials, creating new materials and improving sorghum anthracnose-resistant varieties for sorghum anthracnose-resistant breeding research.

The invention provides a special seedling-stage fertilizer for improving crop drought resistance and promoting growth and a preparation method thereof, and belongs to the technical field of fertilizers. The special fertilizer for the seedling stage comprises the following components in parts by mass: 60-100 parts of a compound fertilizer, 5-15 parts of a water pteris vittata extract, 5-10 parts of a seaweed extract, 3-7 parts of a compound microbial agent, 2-6 parts of proline, 0.1-0.3 part of calcium lignosulphonate and 0.1-0.3 part of tetrasodium glutamate diacetate. The special fertilizer for the seedling stage is suitable for various crops such as corn, soybeans, wheat, flax and sorghum, and the drought resistance and the biological accumulation amount of crop seedlings can be remarkably improved. The invention provides a simple, convenient, economical and effective solution for drought-resistant cultivation of crops, and has a better market application prospect.

The invention relates to the technical field of biological fermentation, in particular to a biological treatment method for sorghum vinasse and application thereof.The biological treatment method comprises the steps that sorghum vinasse, corn flour, soybean meal, wheat bran, ammonium sulfate, monopotassium phosphate, calcium carbonate and sodium bicarbonate are evenly mixed according to the material-water ratio of 1: 1.2, autoclaved sterilization and cooling are conducted, 0.8% of an enzyme preparation is sequentially added, fermentation is conducted for one day at the temperature of 30 DEG C, and then fermentation is conducted for one day at the temperature of 30 DEG C; and adding 9.6% of a complex microbial inoculant again, fermenting at 30 DEG C for 4 days, finally adding 0.06% of lactic acid bacteria, fermenting for 1 day, drying at 45 DEG C, and crushing. The water content in the treated sorghum vinasse is reduced by 6%, the crude protein content is increased by 5%, the neutral detergent fiber content is reduced by 10%, and the acid detergent fiber content is reduced by 8%. The sorghum vinasse prepared by the method disclosed by the invention is used as a protein raw material of the meat duck compound feed, and is added into the meat duck compound feed according to the weight of 10-25%, so that an ideal weight increasing effect can be achieved, the breeding cost is remarkably reduced, and the method is simple to operate and obvious in effect.

The invention discloses a drought-resisting, waterlogging-preventing and fertility-increasing crop diversity utilization system for organic sorghum in mountainous regions and an establishment method thereof, and belongs to the technical field of comprehensive utilization of rainwater. The system comprises a water guide groove, the water guide groove comprises a vertical water guide groove and an equal-height water guide groove, and the vertical water guide groove is connected with the equal-height water guide groove; the filter tank is connected with the vertical water chute; the reservoir is connected with the filter tank; the water tank is connected with the reservoir and used for controlling the water level of the reservoir; plants are planted above the water guide grooves; rainwater and surface runoff flow into the equal-height water guide groove, flow into the filter tank through the vertical water guide groove, flow into the reservoir to be stored after being filtered by the filter tank, and nutrients generated by plants flow into the reservoir along with water flow. The problems that the slope farmland field rainwater resource utilization rate is low, and the waterlogging-preventing and drought-resisting efficiency is low are solved. Waterlogging of crops caused by concentrated rainfall in the mountainous region is reduced, water resources in the mountainous region are reasonably utilized, the plants and the crops are intercropped, and diversity planting of the crops and integration of water and fertilizer are achieved.

A novel sorghum variety designated 2PRQV78R and seed, plants and plant parts thereof are provided. Methods for producing a plant comprise crossing sorghum variety 2PRQV78R with another plant. Sorghum seed, plants and plant parts produced by crossing sorghum variety 2PRQV78R with another plant are described. Methods for producing a plant containing in its genetic material one or more traits introgressed into 2PRQV78R include one or both of backcross conversion and transformation. Hybrid sorghum seed, plants or plant parts produced by crossing the sorghum variety 2PRQV78R or a locus conversion of 2PRQV78R with another sorghum variety are disclosed.

The invention relates to the technical field of molecular marker development and molecular marker assisted breeding, and particularly discloses a KASP molecular marker related to salt tolerance of sorghum, a primer and application of the KASP molecular marker and the primer, the KASP molecular marker is ST1.1 as shown in SEQ ID NO.1, and the molecular marker has T / C polymorphism at a basic group at 100bp of the sequence as shown in SEQ ID NO.1. The KASP molecular marker ST1.1 provided by the invention is used for identifying the salt tolerance of sorghum, through KASP genetic typing detection, the genotype of a sample with a strong FAM fluorescence signal is T / T, and the sample is a salt-tolerant germplasm; the genotype of the sample with strong HEX fluorescence signal is C / C, and the sample is a salt-sensitive germplasm.

The invention relates to the technical field of molecular marker development and molecular marker assisted breeding, and particularly discloses a KASP molecular marker related to sorghum panicle tightness, a primer and application thereof, the KASP molecular marker is at least one of PT1.1 as shown in SEQ ID NO.1 and PT1.2 as shown in SEQ ID NO.2, and basic groups at 25bp positions of molecular marker sequences of PT1.1 and PT1.2 have polymorphism. The molecular marker provided by the invention has relatively high correlation with the sorghum panicle tightness character, rapid, accurate and high-throughput detection of the sorghum panicle tightness can be realized by utilizing the molecular marker on the genotype level, and the marker has an earlier time advantage, higher accuracy and higher speed when being applied to assisted breeding.

The invention discloses application of a sorghum SbMyb1 gene to regulation and control of grain yield, tannin and anthocyanin, and relates to the field of plant genetic engineering. The invention specifically discloses an application of a SbMyb1 gene or a related biological material thereof in regulation and control of plant economic traits. Experiments prove that the SbMyb1 gene encoding the protein with the amino acid sequence shown as SEQ ID NO: 2 can negatively regulate and control yield-related traits such as grain length and grain weight and positively regulate and control the content of secondary metabolites such as grain tannin and anthocyanin, has a good application prospect in high-yield, high-quality and stress-resistant molecular design breeding, and has a wide application prospect. And a new research thought is provided for the SbMyb1 gene to participate in the grain development process through the function exertion of the MBW complex.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker of a gene SbST1 related to sorghum stem diameter and application of the SNP molecular marker. The invention relates to the technical field of molecular biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker of a gene SbST1 related to sorghum stem diameter and application of the SNP molecular marker. By detecting the polymorphism or genotype of SNP1 and SNP2 in the stem diameter gene SbST1 in the sorghum variety to be detected, it is found that the stem diameter mean value of the sorghum variety with the genotype of SNP1 being TT and the genotype of SNP2 being TT is significantly higher than that of the sorghum variety with the genotype of SNP1 being GG and the genotype of SNP2 being AA. The stem diameter of the sorghum variety of 78.05% of the haplotype SbST1-Hap1 is higher than 20 mm. It is shown that the sorghum stem diameter molecular marker of the haplotype SbST1-Hap1 can be used for assisting selective breeding, and the selection efficiency of sorghum high-stem-diameter varieties is remarkably improved.

The invention discloses a method for classifying imperfect sorghum grains based on hyperspectral decision-level data fusion, which comprises the following steps of: taking sorghum as a research object, collecting hyperspectral spectrum information of the sorghum, respectively extracting statistical texture and frequency domain texture of a grayscale image, simultaneously extracting spectral information, analyzing overlapping and difference of characteristic peaks, and determining a characteristic wave band; respectively establishing a random forest and a long and short time sequence network classification model based on various kinds of preprocessed spectrum information and image texture information; on the basis of the baseline model, model classification performance is compared, and a cross-domain feature level fusion model is established; performing feature screening and data dimension reduction on the spectral information and the texture information to establish an intermediate data fusion model; and finally, realizing decision level fusion through Bayesian consensus and a weighting mechanism. Through the data fusion method under different levels, the data dimension is reduced, the model performance and robustness are improved, and an efficient solution is provided for development and practical application of sorghum nondestructive testing.