13 results about "Red kidney beans" patented technology

The invention discloses a preparation method and application of an anti-continuous cropping microbial fertilizer, and aims to solve the problems of aggravation of crop diseases and insect pests and reduction of yield and quality caused by continuous cropping planting. The preparation method comprises the following steps: activating and uniformly mixing bacillus subtilis and bacillus licheniformis, and adding an alpha-lactalbumin solution and a calcium chloride solution to prepare a bacillus aggregate; adding bentonite into the polytrehalose solution, and performing ultrasonic treatment on the mixture and the humic acid solution to prepare a modified polytrehalose-bentonite compound; uniformly mixing acremonium terricola, trichoderma harzianum and a bacillus aggregate, and adding a wall material solution to prepare a compound bacterium microcapsule preparation; and finally, mixing the microcapsules with the corn straw biochar, the chitosan oligosaccharide and the like to obtain a finished product. Through calcium ion induction and humic acid modified embedding, the strain stability and viable count are improved, soil organic matter and nitrogen phosphorus and potassium nutrients can be activated, pathogenic bacteria are inhibited, the crop rhizosphere environment is improved, the growth index and quality of red kidney beans and potatoes are effectively improved, and the method is suitable for continuous cropping resistant planting of crops.

The invention provides application of delphinidin-3-glucoside in preparation of uric acid reducing drugs or functional products, and belongs to the technical field of medical application. According to the invention, a novel component-target-metabolic pathway (C-T-M) network is constructed, and the network provides a comprehensive method for analyzing a specific component target and the effect of the specific component target in influencing the metabolic pathway of the HUA. Molecular dynamics (MD) simulation and surface plasmon resonance (SPR) technologies are adopted, and in-vitro and in-vivo metabonomics researches are carried out. The invention discloses a uric acid reducing effect of Dp-3G in red kidney beans, and the uric acid reducing effect is realized by combining with a flavin adenine dinucleotide (FAD) site of xanthine oxidase (XO) enzyme. Therefore, the research provides a new insight for preventing and treating the HUA by specific beans and key natural medicines.

The present application belongs to the technical field of plant active peptides, and particularly relates to an active peptide with anti-inflammatory and immune-regulating functions and application thereof. In order to provide a new bioactive peptide, polypeptides are prepared by protease hydrolysis of red kidney bean, and the enzyme-hydrolyzed polypeptides with a molecular weight less than 3 kDa are separated by ultrafiltration, and the polypeptide sequences are identified by LC-MS / MS. The active peptides FL and YF are screened by bioinformatics and molecular docking, and the amino acid sequences of the active peptides are FKSIGVKPPKGILL and YLFPESPARLPGF respectively. The active peptides can improve the survival rate, NO release amount, phagocytic capacity and cytokine production of RAW264.7 macrophages, can obviously inhibit the NO release amount of RAW264.7 cells induced by LPS, can inhibit the expression of inflammatory factors iNOS, TNF-alpha and IL-6, and can be applied to the preparation of anti-inflammatory or immune-regulating functional food or drugs.

The invention belongs to the technical field of phytopathogen detection. The invention provides a multiplex PCR (Polymerase Chain Reaction) primer combination which comprises a common upstream primer FusF, and the sequence of the upstream primer is SEQ ID NO.1; and downstream primers FoxR, FeqR and FtrR, and the sequences of the downstream primers are SEQ ID NO.2-4. The three pathogenic bacteria of the red kidney bean root rot are fusarium oxysporum, fusarium equiseti and fusarium tritrichum respectively, the upstream primer and the downstream primer are combined respectively, the primer pair of FusF / FoxR is a primer pair for identifying or assisting in identifying the fusarium oxysporum, the primer pair of FusF / FeqR is a primer pair for identifying or assisting in identifying the fusarium equiseti, and the primer pair of FusF / FeqR is a primer pair for identifying or assisting in identifying the fusarium tritrichum. The primer pair of the FusF / FtrR is a primer pair for identifying or assisting in identifying the fusarium trisporum; the invention also provides application of the multiplex PCR primer combination in detection of three pathogenic bacteria of red kidney bean root rot and a detection method. The method has strong specificity and high efficiency, and can provide reliable technical support for diagnosis and prevention of red kidney bean root rot.

The application provides a red kidney bean functional peptide, a red kidney bean active peptide and a preparation method and application thereof, and belongs to the fields of biological medicine and food technology. The red kidney bean functional peptide comprises at least one of the following polypeptides: peptide 1, peptide 2 and peptide 3; the amino acid sequence of the peptide 1 is LPLF; the amino acid sequence of the peptide 2 is FLS; and the amino acid sequence of the peptide 3 is WYG. The application provides a preparation method of the red kidney bean active peptide, comprising the following steps: after broken red kidney beans are treated with alkali, the broken red kidney beans are subjected to first enzymolysis by using cellulase and saccharifying enzyme, and then subjected to second enzymolysis by using alkaline protease; and a component with a molecular weight of 3-10 kDa is collected to obtain the red kidney bean active peptide. The red kidney bean functional peptide and the red kidney bean active peptide can regulate blood lipids, regulate blood sugar and relieve oxidative stress, and have a good application prospect in the fields of functional food or medicine.

The present application relates to a kind of red kidney bean bacteriostatic active polypeptide and its preparation method.The preparation method includes the following preparation steps: S100, raw material pretreatment: the red kidney bean dreg of extracting albumin is softened and grinded homogenization to be made into slurry by adding water;S200, hydrolysis: two kinds of neutral protease hydrolysis are added to the slurry, to obtain hydrolysate;S300, membrane separation: the hydrolysate is inactivated after enzyme, and solid-liquid separation is carried out, and the obtained filtrate is separated using the ultrafiltration membrane with the molecular weight cut-off 3000Da, and the obtained ultrafiltrate is desalted and concentrated using the nanofiltration membrane with the molecular weight cut-off 300Da, to obtain red kidney bean bacteriostatic active polypeptide concentrate.The polypeptide prepared by the method of the present application has inhibitory effect on streptococcus mutans, salmonella and staphylococcus aureus.Because these bacteria are common digestive tract pathogenic bacteria, the polypeptide can be used as a product for inhibiting harmful bacteria in digestive tract or as an external bacteriostatic product.

The invention belongs to the technical field of compound fertilizers, and particularly relates to a compound fertilizer for red kidney beans as well as a preparation method and application thereof. The compound fertilizer adopts a core fertilizer-coating structure, and the core of the compound fertilizer is that the core fertilizer comprises a composite microbial inoculum which is prepared by compounding rhizobium RH64 and lysinibacillus fusiformis according to the concentration ratio of 1: 2-2: 1, and synergistic urea, diammonium phosphate, potassium chloride, borax, sodium molybdate, fulvic acid and other nutritional and functional components. Field test results show that the compound fertilizer significantly improves nitrogenase activity of root nodules of red kidney beans, thereby effectively promoting growth of the red kidney beans, achieving great yield increase, and significantly increasing crude protein and total dietary fiber content in grains. The invention provides an efficient and reliable solution for solving the continuous cropping obstacle of the red kidney beans and improving the yield and quality of the red kidney beans.

The invention relates to composite instant fish maw and a preparation method thereof, and belongs to the field of fish maw products, and the composite instant fish maw comprises the following substances in parts by weight: 20-30 parts of sciaenops ocellatus fish maw, 10-14 parts of red kidney beans, 10-20 parts of small red beans, 6-10 parts of red dates, 2-4 parts of coix seeds, 2-3 parts of a composite additive solution, 2-4 parts of brown sugar and 40-60 parts of purified water. According to the invention, the selected sciaenops ocellatus fish maw is not only rich in collagen, but also contains type 6 collagen, and the type 6 collagen is unique collagen and plays an important role in uterus at different stages. According to the invention, the composite additive solution, the fish maw, the red kidney beans, the small red beans, the red dates, the coix seeds and the brown sugar have a synergistic effect; the traditional Chinese medicine composition has the effects of warming and nourishing uterus and nourishing qi and blood, and further enhances the nourishing and health-care functions on qi and blood, liver and kidney and the like of women while providing basic nutrition, so that the effects of nourishing uterus and protecting nests are enhanced, and the diversified requirements of female consumers on health and health preservation are met.

The invention belongs to the field of polymer compositions for mulching films, and particularly relates to a modified polylactic acid degradable mulching film for red kidney bean planting and a preparation method thereof. The modified polylactic acid degradable mulching film is prepared from the following raw materials in parts by mass: 40 to 60 parts of polylactic acid, 40 to 60 parts of poly (butylene adipate-co-terephthalate), 15 to 25 parts of drug-loaded nano microspheres, 4 to 6 parts of a coupling agent modified degradation promoting agent and 1 to 3 parts of a lubricating agent, adding the drug-loaded nano-microspheres into water through phenolated lignin to adjust the pH value, adding a pore-foaming agent and a cross-linking agent, and preparing a water phase; uniformly mixing the water phase and the oil phase for reaction to obtain porous nano microspheres; and soaking the porous nano-microspheres in a pesticide solution for adsorption, and adding a coating solution. The mulching film is high in mechanical property; and the drug-loaded nano microspheres and the coupling agent modified degradation promoting agent cooperate to enable the degradation rate of the mulching film to be matched with the growth cycle of the red kidney beans.

Fusarium equiseti can cause root rot of many crops including red kidney beans, which seriously affects crop yield and quality. The present application selects CYP51C gene as a target to provide a LAMP primer composition for detecting Fusarium equiseti, a detection kit and application, and establishes a LAMP detection technology system for Fusarium equiseti. The technology can realize the rapid detection of Fusarium equiseti within 22 min, and the detection limit is 10 pg / μL. The method provides strong technical support for the field rapid diagnosis of red kidney bean root rot.

This invention relates to the field of monitoring and control technology, specifically disclosing a monitoring system and method for diseases of mixed beans, including a UAV remote sensing acquisition module, an image preprocessing and feature extraction module, an expert teaching and path recording module, a trajectory replay and servo execution module, a gradient ensemble classification module, a dynamic abnormal sample mining and adaptive reinforcement learning module, and a visualization and control scheduling module. This invention achieves spatial localization, hierarchical identification, and closed-loop scheduling of control tasks for CBB lesions in red kidney beans at different stages (early, middle, and late) through hyperspectral multi-resolution data acquisition, expert interactive path standardization script generation and automatic reproduction, priority capture of dynamic abnormal samples and multi-terminal expert remote collaborative verification, feature screening and gradient ensemble modeling, and intelligent generation of variable prescription maps.

The application belongs to the field of polymer compositions for mulching films, and particularly relates to a modified polylactic acid degradable mulching film for red kidney bean planting and a preparation method thereof. The modified polylactic acid degradable mulching film comprises the following raw materials in parts by mass: 40-60 parts of polylactic acid, 40-60 parts of polybutylene adipate terephthalate, 15-25 parts of drug-loaded nanometer microspheres, 4-6 parts of coupling agent modified degradation promoter, and 1-3 parts of lubricant. The drug-loaded nanometer microspheres are prepared by adding phenolated lignin into water to adjust pH, adding a pore former and a crosslinking agent, and preparing an aqueous phase; the aqueous phase and an oil phase are mixed and reacted to obtain porous nanometer microspheres; the porous nanometer microspheres are immersed in a pesticide solution for adsorption, and a coating liquid is added to obtain the modified polylactic acid degradable mulching film. The mulching film has strong mechanical properties; and the drug-loaded nanometer microspheres and the coupling agent modified degradation promoter are used in combination to match the degradation rate of the mulching film with the growth cycle of red kidney beans.