49 results about "Arachis hypogaea" patented technology

The invention discloses a KASP molecular marker related to brassica napus quality traits and application of the KASP molecular marker, and belongs to the technical field of molecular genetics. According to the KASP molecular marker related to the brassica napus quality traits, the primers of the KASP molecular marker and the application of the KASP molecular marker and the primers in brassica napus genetic typing or quality traits identification, results show that the KASP molecular marker can accurately identify genotypes, is high in sensitivity and specificity and effectively reduces the error rate. The invention also discloses a brassica napus genotyping / quality identification method, and the result shows that the material with the genotyping result of AA has the characteristics of low erucic acid content, low thioglycoside content, high oleic acid content, high linoleic acid content, low arachidonic acid content and low protein content; gG-type materials exhibit high erucic acid, high thioglycoside, low oleic acid, low linoleic acid, high arachidonic acid and high protein content. Individuals with excellent quality characters can be rapidly screened in early generations, and the long process of character identification after plant maturation in traditional breeding is avoided.

The invention discloses a molecular marker closely linked with peanut yield related traits and application, and belongs to the technical field of plant molecular genetics and molecular breeding. The molecular marker disclosed by the invention is Arahy0036; the nucleotide sequence of Arahy0036 in peanuts with low single plant yield is shown as SEQ ID NO: 1, and the nucleotide sequence of Arahy0036 in peanuts with high single plant yield is shown as SEQ ID NO: 2. The marker is used for detecting the gene locus related to the yield character of the peanut single plant, the single plant with high yield can be identified after seeds are harvested, other single plants are selected, reserved and eliminated, the breeding scale can be effectively controlled, and the progress and efficiency of variety selection breeding are improved.

The invention discloses a streptomyces albidoflavus strain SaTC2601 and application thereof, and belongs to the field of biological control. According to the invention, a streptomyces albidoflavus strain SaTC2601 is separated from faeces of larvae of WSFC, and the preservation number of the streptomyces albidoflavus strain SaTC2601 is CGMCC (China General Microbiological Culture Collection Center) No.33741. The strain SaTC2601 has a very good prevention and treatment effect on peanut southern blight caused by sclerotium rolfsii at peanut roots, also has a prevention and treatment effect on soil-borne pathogenic bacteria such as fusarium graminearum, fusarium solani, fusarium moniliforme, fusarium oxysporum, rhizoctonia solani and streptomyces scabies, and further, the strain SaTC2601 also has a very good growth promoting effect. The strain becomes a precious resource for new drug development and agricultural disease prevention and control, and is expected to play a huge role in the aspect of soil disease prevention and control.

This invention relates to the field of microbial technology, specifically to a compound bacterial agent, its preparation method, and its application, wherein the active ingredient is Bacillus arachidica (B. arachidica). Peanut Bacillus CH-RS16 and cold-resistant *Bacillus subtilis* ( Peribacillus frigoritolerans ZM-RS6. The compound microbial agent provided by this invention promotes the growth and development of wheat and improves its drought resistance.

The invention discloses a compound grease feed additive for improving breeding quality of channa maculata and application. The method comprises the following steps: (1) preparing experimental feed; the method comprises the following steps: respectively preparing an experimental group ARA 1.5 added with 1.5% of arachidonic acid and an experimental group ARA + PC added with 1.5% of arachidonic acid and 1.5% of phosphatidylcholine; (2) feeding; (3) after the culture experiment is finished, treating and detecting the channa maculata; (4) carrying out an artificial propagation test; (5) carrying out ovarian steroid synthesis related gene expression detection; and (6) carrying out data statistical analysis. By adding arachidonic acid (ARA) and phosphatidylcholine (PC) into the feed, the breeding performance of channa maculata and the quality of fry are improved, and the feed has very important significance for improving the aquatic product efficiency of high-quality fry of channa maculata in aquaculture.

The invention discloses a molecular marker closely linked with peanut pod size character, a primer pair and application, and belongs to the technical field of plant molecular genetics and molecular breeding. The molecular marker disclosed by the invention is Arahy0055; the nucleotide sequence of the Arahy0055 in the peanuts with small pods is as shown in SEQ ID NO: 1, and the nucleotide sequence of the Arahy0055 in the peanuts with large pods is as shown in SEQ ID NO: 2. The marker is used for detecting gene loci related to the size character of the peanut pods, single plants of the large pods can be identified after seeds are harvested, other single plants are selected, reserved and eliminated, the breeding scale can be effectively controlled, and the progress and efficiency of variety selection breeding can be improved.

The invention discloses a synergistic attractant for Holotrichia parallela and Artemisia cordata and a pheromone light source trapping method of the synergistic attractant, and belongs to the technical field of pest trapping. On the basis of sex attractants (L-isoleucine methyl ester and linalool), diethyl phthalate and benzyl alcohol are added, a synergistic attractant with a remarkable attraction effect on holotrichia parallela and ponceau is formed, and all the components have a synergistic effect. Meanwhile, the synergistic attractant has a low attraction effect on field dominant natural enemies such as harmonia axyridis, orius minutus and propylaea japonica in the growth period of peanuts, and is very friendly. Besides, a synergistic attractant and light trapping (380-385nm) are combined, so that more remarkable trapping of the holotrichia parallela and the largetria corpulenta is realized, and an efficient and environment-friendly pheromone light trapping technology is finally developed. The trapping technology has an excellent trapping effect on holotrichia parallela and Lonicera corpulenta, and is friendly to natural enemies.

The present invention belongs to the technical field of genetic engineering, and particularly relates to an GmHMGS application of a gene and a recombinant overexpression vector in regulating the fatty acid content of plants, and the GmHMGS nucleotide sequence of the gene is shown as SEQ ID NO.1. The present invention uses an overexpression vector of an exogenous gene sequence to introduce the GmHMGS gene shown as SEQ ID NO.1 into plants for overexpression GmHMGS of the gene, and the plants show that the contents of palmitoleic acid, arachidonic acid, docosanoic acid and erucic acid are significantly higher than those of the wild type; at the same time, the contents of stearic acid, oleic acid and linolenic acid are significantly reduced. The present invention regulates the GmHMGS expression of the gene in plants and then uses it to cultivate new plant varieties with adjustable fatty acid content, which provides a new idea for cultivating new crop varieties and has great application value in molecular breeding.

The application discloses application of Fagopyrum cymosum root powder in preparation of products for improving egg quality or intestinal health of laying ducks, and belongs to the technical field of feed additives. The application provides application of Fagopyrum cymosum root powder in preparation of products for improving egg quality or intestinal health of laying ducks, and the Fagopyrum cymosum root powder can improve eggshell strength, yolk color and eggshell proportion, improve the content of aspartic acid, threonine, glutamic acid, glycine, cystine and leucine in yolk, improve the content of arachidonic acid and arachidonic acid in yolk, and reduce the content of saturated fatty acid in yolk; the Fagopyrum cymosum root powder can improve the activity of maltase in the mucous membrane of the jejunum of laying ducks, and can improve the diversity and abundance of intestinal flora of laying ducks. The application provides a new strategy for improving egg quality or intestinal health of laying ducks.

The invention provides application of arachidonic acid in delaying reproductive aging, and particularly relates to the technical field of reproductive health. The invention provides application of arachidonic acid in preparation of a product for delaying reproductive aging and / or prolonging the reproductive period. By taking caenorhabditis elegans and mice as examples, the arachidonic acid can obviously activate reproductive aging gonad lysosome, protect oocyte quality and prolong reproductive cycle, thereby delaying reproductive aging. The invention provides a new application prospect of arachidonic acid in the aspects of delaying ovarian aging and protecting fertility.

The invention discloses Erwinia americana and application of the Erwinia americana in prevention and treatment of plant pathogenic fungi, and belongs to the technical field of agriculture. The Ewingella americana disclosed by the invention is Ewingella americana NJC80 and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M2025462, and the preservation date is March 13, 2025. The Ewingella americana (Ewingella americana) NJC80 has an inhibition effect on plant pathogenic fungus alternaria alternata (Alternania alternata), and can be used for preparing a preparation for preventing the peanut leaf spot disease.

The invention provides a feed additive for improving the hypoxia tolerance function of micropterus salmoides. The feed additive is prepared from the following raw materials in percentage by mass: 20% of arachidonic acid and the balance of maltodextrin. The invention also provides a feed composition of the feed additive. The feed is prepared from the following raw materials in percentage by mass: 450 per mill of fish meal, 50 per mill of fermented hermetia illucens pulp, 69.5 per mill of enzymolysis chicken powder, 60 per mill of chicken liver powder, 60 per mill of enzymolysis soybean meal, 75 per mill of fermented soybean meal, 60 per mill of cottonseed meal, 29 per mill of dextrin, 40.5 per mill of cassava starch, 23 per mill of soybean oil, 23 per mill of linseed oil, 15 per mill of monocalcium phosphate, 5 per mill of choline chloride, 1 per mill of a micropterus salmoides premix, 1 per mill of zeolite powder and 20 per mill of a feed additive. The feed composition is used for improving the hypoxia tolerance of the micropterus salmoides. According to the invention, arachidonic acid is used as a functional active substance to prepare the hypoxia stress resistant functional feed, and the addition of arachidonic acid can significantly activate liver angiogenesis genes, promote the liver angiogenesis level and improve the hypoxia tolerance of largemouth bass.

The invention discloses a method for identifying the specificity of red seed coat peanuts based on high antioxidant active substances, which is used for identifying or identifying peeled peanut materials or other industrial and agricultural peanut materials, and constructs a specific chromatographic analysis path based on newly found specific chemical components in the red seed coat peanuts. And the red seed coat peanut raw material is identified and identified based on the specific characteristic chromatographic peak of the red seed coat peanut material. For common peanut protein powder, peeled peanut powder, peeled peanut seed kernels and other common materials, the method can directly identify whether the agricultural and industrial peanut raw materials are red-seed-coat peanut sources or not without depending on the integrity of seed coats, and an important technical basis is provided for quality control and variety identification of the peanut raw materials.

The invention discloses a synergistic composition and application thereof in prevention and treatment of peanut diseases, and relates to the technical field of pesticides. Comprising physcion and thifluzamide. The bactericidal composition is applied to prevention and treatment of peanut southern blight caused by sclerotium rolfsii, peanut root rot caused by fusarium solani and focal spot caused by pholiota arachida. The synergistic composition provided by the invention has a great application prospect in prevention and treatment of diseases such as southern blight, root rot and focal spot of peanuts.

The present application relates to a kind of methods for improving the biological activity of Bacillus thuringiensis preparation using organic acid.The method is specifically as follows: adding organic acid to Bacillus thuringiensis preparation, thereby improving the biological activity of Bacillus thuringiensis preparation;The Bacillus thuringiensis preparation is Bacillus thuringiensis wettable powder;The organic acid is selected from any one or more of palmitic acid, stearic acid, arachidic acid, acetylsalicylic acid and oleic acid;The biological activity includes any one or more of biofilm formation ability, ultraviolet resistance and insecticidal activity.By the method of the present application, the application effect of Bacillus thuringiensis preparation in agriculture can be significantly improved, and has high practical application value.

The invention belongs to the technical field of peanut cultivation methods, and particularly relates to a method for high-density shake-flask fermentation of bradyrhizobium anhuoshanense and application of the bradyrhizobium anhuoshanense. The improved YGA culture medium is used for carrying out shake flask fermentation on the peanut bradyrhizobium strain WYCCWR 15459, the high-density peanut bradyrhizobium inoculant is prepared, the effective viable count of the inoculant is larger than 10 < 11 > cfu / mL, after fermentation is carried out for 168 h, the inoculant shows significant difference from a YMA culture medium, an MYMA culture medium, a TY culture medium, a YMAR culture medium, a ZJC culture medium and a YDS culture medium, and finally OD600 is 1.26 times that of the YMA culture medium. By optimizing the formula and fermentation conditions of the YGA culture medium, high-density shake flask fermentation of the bradyrhizobium anhuoshanense microbial inoculum is achieved, and the preparation method is efficient, environmentally friendly, simple in technological process and relatively low in cost and has production and application potential.

This invention belongs to the field of pesticide technology, specifically relating to a pesticide composition containing benzimidazole. The pesticide composition's active ingredient is a compound of benzimidazole and fluazinam or emodin methyl ether. This invention, through indoor bioactivity tests, found that the compound of benzimidazole with fluazinam or emodin methyl ether, within a certain mass ratio range, has a synergistic effect against various plant fungal diseases such as Rhizoctonia solani, Curvularia crescentis, and Sclerotium sclerotiorum, helping to improve control efficacy. This is of great significance for the research and development of pesticides for controlling diseases such as cucumber damping-off, corn curvularia leaf spot, and peanut white mold.

The invention belongs to the technical field of biology, and particularly relates to a method for culturing fucoxanthine, oleic acid and arachidonic acid to increase the accumulation amount of fucoxanthine, oleic acid and arachidonic acid. According to the method, the microcystis is added into the palmitotheca for mixed culture, and meanwhile, continuous inflation is performed, so that the microcystis is swallowed by the palmitotheca. The mode of swallowing the microcystis by utilizing the palmitothecium is utilized to realize higher fucoxanthine accumulation in the palmitothecium. The experiment proves that the palmitotheca has high removal efficiency on non-toxic microcystis, and can effectively ingest toxic microcystis, so that a new way is provided for biomanufacturing of fucoxanthine with higher accumulation amount.

The invention provides a thrombocytopenia accompanied with thrombus zebrafish model as well as a construction method and application thereof, belongs to the technical field of biology, and particularly relates to a thrombocytopenia accompanied with thrombus zebrafish model, which is characterized in that CALR del52 is over-expressed in zebrafish somatic cells to obtain transgenic zebrafish; adding an arachidonic acid thrombus inducer into the transgenic zebrafish to induce construction; the nucleotide sequence of the CALR del52 is as shown in SEQ ID NO. 1; the zebrafish model with thrombocytopenia associated with thrombosis disease provided by the invention can be used for screening antithrombotic therapeutic drugs, is short in experimental period and low in dosage, and is beneficial to high-throughput screening of trace antithrombotic active natural products.

The application provides a mycoplasma hyopneumoniae culture medium capable of significantly improving the strain titer and an application thereof, and belongs to the field of veterinary biological products. The mycoplasma hyopneumoniae culture medium is obtained by adding L-asparagine, myo-inositol, arachidonic acid, urea, histamine, sodium pyruvate, valproic acid, glyceric acid, lysophosphatidic acid, L-citrulline, L-glutamine and L-leucine into a KM2 culture medium containing Bama pig serum. The culture medium can significantly improve the growth rate and viable bacterial titer of the mycoplasma hyopneumoniae under a low serum concentration, and reduces the production cost.

The application discloses a KASP molecular marker related to quality traits of Brassica napus and application thereof, and belongs to the technical field of molecular genetics.The application discloses the KASP molecular marker related to quality traits of Brassica napus and a primer thereof, and application of the KASP molecular marker and the primer in genotyping or quality trait identification of Brassica napus.The results show that the application can accurately identify genotypes, has high sensitivity and specificity, and effectively reduces the error rate.The application further discloses a Brassica napus genotyping / quality identification method.The results show that a material with a genotyping result of AA has the characteristics of low erucic acid, low glucosinolate, high oleic acid, high linoleic acid, low arachidic acid and low protein content; a material with a GG type shows high erucic acid, high glucosinolate, low oleic acid, low linoleic acid, high arachidic acid and high protein content.The application can quickly screen out individuals with excellent quality traits in early generations, and avoids the long process of identifying traits after plants mature in traditional breeding.

The invention discloses a feed additive composition as well as a preparation method and application thereof, and belongs to the technical field of sturgeon culture. The feed additive composition comprises arachidonic acid, vitamin C and pollen. Besides, the invention also provides a preparation method of the feed additive composition, which comprises the following steps: mixing the selenium yeast, the first starch and the first water, drying to obtain first particles, and mixing the first particles, the arachidonic acid, the vitamin C and the pollen to obtain the feed additive composition. In addition, the invention further provides application of the feed additive composition in sturgeon feed. According to the feed additive composition provided by the invention, the immunity of sturgeons can be effectively improved under the cooperation of the arachidonic acid, the vitamin C and the pollen.

This invention discloses a method for increasing the yield of arachidonic acid (RAA) produced by *Morchella spp.* fermentation, relating to microbial fermentation technology. The method employs either solid-state fermentation or liquid-state shallow static fermentation. *Morchella spp.*, used for RAA synthesis, is inoculated onto the surface of a solid-state fermentation medium for solid-state fermentation, or inoculated into a liquid fermentation medium for liquid-state shallow static fermentation. Feeding is performed during fermentation until the RAA content in the *Morchella spp.* reaches its peak. This method can increase the RAA content in synthesized oils to 1.72 times that of deep fermentation, reaching a maximum of 80.93%. The yield of RAA synthesized by *Morchella spp.* per 1L of culture medium consumed is up to four times that of deep fermentation. This method requires less equipment investment, is simple to operate, has very low energy consumption, is pollution-free, and allows for large-scale production of RA-rich oils and microorganisms, showing broad application prospects in pharmaceutical intermediates, nutritional supplements, and animal feed.

The application provides a thrombocytosis and thrombosis zebrafish model and a construction method and application thereof, and belongs to the technical field of biology. CALR del52 The transgenic zebrafish is obtained, and the transgenic zebrafish is added with a thrombus inducer of arachidonic acid to induce and construct; CALR del52 The nucleotide sequence is shown as SEQ ID NO. 1; the thrombocytosis and thrombosis disease zebrafish model provided by the application can be used for screening of anti-thrombus treatment drugs, has a short experimental cycle, a small dosage, and is favorable for high-throughput screening of trace anti-thrombus active natural products.

The application discloses a method for improving egg quality by using peony and a feed additive, and belongs to the field of animal husbandry. The method for improving egg quality comprises adding 0.75-1.5% of peony stem and leaf powder in the basic daily ration of laying hens. The method can significantly improve the production performance of laying hens in the later production stage, and the effect is better than that of aureomycin. Meanwhile, the method can also significantly enhance the antioxidation ability of the body, improve the enrichment of MUFA, gamma-linolenic acid and arachidonic acid in eggs, and has the potential to replace feed antibiotics.

This invention belongs to the field of green pest control technology in agriculture, and provides a method for controlling underground pests in peanuts: before crop sowing, previously planted Cnidium monnieri is pressed into the soil as green foliage; the sowing rate of Cnidium monnieri is 1-6.5 kg per mu, and the amount of green foliage returned to the soil is 7000-37500 kg / ha fresh weight, with the green foliage pressing occurring 5-10 days before crop sowing. The preferred underground pests are grubs, wireworms, and cutworms. This invention regulates the soil ecological environment through agronomic measures, which can increase the enrichment level of Metarhizium anisopliae in the soil and repel grubs, thereby reducing the occurrence of underground pests and mitigating yield losses caused by them. This method does not require the application of additional chemical agents specifically for underground pests and has good ecological and application value.

The invention discloses application of arachidonic acid in induction improvement of corn leukoderma resistance, the arachidonic acid is applied to prevention and treatment of corn leukoderma for the first time, and experiments show that the arachidonic acid can improve the photosynthetic effective leaf area, Fm content, Fv / Fm content, NPQ content, soluble sugar content and soluble protein content of plants; and / or increasing the activity of disease-resistant-related defensive substances; and / or catalyzing synthesis of lignin and phenolic substances; and / or the content of Rfd, MDA and reducing sugar of the plant is reduced to induce and improve the corn leukoderma resistance, so that a new direction and basis can be provided for preventing and treating the corn leukoderma by adopting natural compounds. It is found that the arachidonic acid does not strictly depend on the total spraying amount for inducing and improving the leukoderma resistance, but when the spraying amount is the same and the concentration of the arachidonic acid in the spraying liquid is 15 [mu] M, the arachidonic acid is optimal for inducing and improving the leukoderma resistance. And the application of arachidonic acid is beneficial to reducing the particle size of starch particles in corn kernels under the pressure of leukoderma.

The present application relates to a kind of genetic locus related to peanut leaf size, gene and molecular marker, belong to crop molecular genetics and molecular marker technical field.The two genetic loci related to leaf size of the present application are LA115165101 and LA115027188, wherein LA115165101 is located at the interval of 115165101-115165105 of the 5th chromosome of peanut, is base CGCTC or deletion;LA115027188 is located at 115027188 of the 5th chromosome of peanut, base is C or T;Two genes related to leaf size of the present application are Arahy.09KEBU and Arahy.PRU9CS, two functional KASP molecular markers are developed based on the gene, using the molecular marker can efficiently, accurately distinguish the leaf size trait of peanut, can effectively distinguish large leaf strain and small leaf strain in population, with the characteristics of simple operation, reliable result, low cost, suitable for large-scale molecular marker assisted selection, provide important technical support for cultivating ideal leaf shape, high yield and stable yield of new peanut variety.

The present application belongs to the field of biotechnology, and particularly relates to a method for improving accumulation of fucoxanthin, oleic acid and arachidonic acid in brown flagellate culture. The method is to add microcystis to brown flagellate for mixed culture, and continuously aerate, and the microcystis is phagocytosed by the brown flagellate. The method of phagocytosis of microcystis by brown flagellate is used to realize higher accumulation of fucoxanthin in brown flagellate. Through the experiment, it is proved that the brown flagellate has higher removal efficiency for non-toxic microcystis, and can also effectively ingest toxic microcystis, and a new way for obtaining higher accumulation of fucoxanthin in biological manufacturing is provided.

The application provides a method for promoting synchronous maturation of parent shrimps, which comprises the following steps: temporarily breeding male parent shrimps and female parent shrimps in a pool; adjusting water flow velocity to perform water flow domestication and temperature domestication, setting the water flow velocity to be 0.4-1.5 m / s, and feeding nutrition enhancer to perform intensive feeding when the water flow domestication is performed; the nutrition enhancer comprises the following components: taurine, arachidonic acid, phosphatidylserine and cuttlefish bone powder; setting the temperature to be 18-19 DEG C, increasing the temperature by 0.4-0.6 DEG C per day, and increasing the temperature to 27-28 DEG C. The water flow domestication and the temperature domestication are performed by adjusting the water flow velocity, and the intensive feeding of the nutrition enhancer is combined when the water flow domestication is performed, which is beneficial to the accumulation of nutritional substances in the hepatopancreas tissue of the female shrimps, promotes synchronous development and maturation of the ovary, and can make the maturation rate of the female shrimps reach 100%, and the synchronous maturation rate reach 80%.