21 results about "Arachis hypogaea" patented technology

This invention relates to the field of microbial technology, specifically to a compound bacterial agent, its preparation method, and its application, wherein the active ingredient is Bacillus arachidica (B. arachidica). Peanut Bacillus CH-RS16 and cold-resistant *Bacillus subtilis* ( Peribacillus frigoritolerans ZM-RS6. The compound microbial agent provided by this invention promotes the growth and development of wheat and improves its drought resistance.

The invention discloses a compound grease feed additive for improving breeding quality of channa maculata and application. The method comprises the following steps: (1) preparing experimental feed; the method comprises the following steps: respectively preparing an experimental group ARA 1.5 added with 1.5% of arachidonic acid and an experimental group ARA + PC added with 1.5% of arachidonic acid and 1.5% of phosphatidylcholine; (2) feeding; (3) after the culture experiment is finished, treating and detecting the channa maculata; (4) carrying out an artificial propagation test; (5) carrying out ovarian steroid synthesis related gene expression detection; and (6) carrying out data statistical analysis. By adding arachidonic acid (ARA) and phosphatidylcholine (PC) into the feed, the breeding performance of channa maculata and the quality of fry are improved, and the feed has very important significance for improving the aquatic product efficiency of high-quality fry of channa maculata in aquaculture.

The application discloses application of Fagopyrum cymosum root powder in preparation of products for improving egg quality or intestinal health of laying ducks, and belongs to the technical field of feed additives. The application provides application of Fagopyrum cymosum root powder in preparation of products for improving egg quality or intestinal health of laying ducks, and the Fagopyrum cymosum root powder can improve eggshell strength, yolk color and eggshell proportion, improve the content of aspartic acid, threonine, glutamic acid, glycine, cystine and leucine in yolk, improve the content of arachidonic acid and arachidonic acid in yolk, and reduce the content of saturated fatty acid in yolk; the Fagopyrum cymosum root powder can improve the activity of maltase in the mucous membrane of the jejunum of laying ducks, and can improve the diversity and abundance of intestinal flora of laying ducks. The application provides a new strategy for improving egg quality or intestinal health of laying ducks.

The invention discloses a method for identifying the specificity of red seed coat peanuts based on high antioxidant active substances, which is used for identifying or identifying peeled peanut materials or other industrial and agricultural peanut materials, and constructs a specific chromatographic analysis path based on newly found specific chemical components in the red seed coat peanuts. And the red seed coat peanut raw material is identified and identified based on the specific characteristic chromatographic peak of the red seed coat peanut material. For common peanut protein powder, peeled peanut powder, peeled peanut seed kernels and other common materials, the method can directly identify whether the agricultural and industrial peanut raw materials are red-seed-coat peanut sources or not without depending on the integrity of seed coats, and an important technical basis is provided for quality control and variety identification of the peanut raw materials.

The invention discloses a synergistic composition and application thereof in prevention and treatment of peanut diseases, and relates to the technical field of pesticides. Comprising physcion and thifluzamide. The bactericidal composition is applied to prevention and treatment of peanut southern blight caused by sclerotium rolfsii, peanut root rot caused by fusarium solani and focal spot caused by pholiota arachida. The synergistic composition provided by the invention has a great application prospect in prevention and treatment of diseases such as southern blight, root rot and focal spot of peanuts.

This invention belongs to the field of pesticide technology, specifically relating to a pesticide composition containing benzimidazole. The pesticide composition's active ingredient is a compound of benzimidazole and fluazinam or emodin methyl ether. This invention, through indoor bioactivity tests, found that the compound of benzimidazole with fluazinam or emodin methyl ether, within a certain mass ratio range, has a synergistic effect against various plant fungal diseases such as Rhizoctonia solani, Curvularia crescentis, and Sclerotium sclerotiorum, helping to improve control efficacy. This is of great significance for the research and development of pesticides for controlling diseases such as cucumber damping-off, corn curvularia leaf spot, and peanut white mold.

The application provides a mycoplasma hyopneumoniae culture medium capable of significantly improving the strain titer and an application thereof, and belongs to the field of veterinary biological products. The mycoplasma hyopneumoniae culture medium is obtained by adding L-asparagine, myo-inositol, arachidonic acid, urea, histamine, sodium pyruvate, valproic acid, glyceric acid, lysophosphatidic acid, L-citrulline, L-glutamine and L-leucine into a KM2 culture medium containing Bama pig serum. The culture medium can significantly improve the growth rate and viable bacterial titer of the mycoplasma hyopneumoniae under a low serum concentration, and reduces the production cost.

The application discloses a KASP molecular marker related to quality traits of Brassica napus and application thereof, and belongs to the technical field of molecular genetics.The application discloses the KASP molecular marker related to quality traits of Brassica napus and a primer thereof, and application of the KASP molecular marker and the primer in genotyping or quality trait identification of Brassica napus.The results show that the application can accurately identify genotypes, has high sensitivity and specificity, and effectively reduces the error rate.The application further discloses a Brassica napus genotyping / quality identification method.The results show that a material with a genotyping result of AA has the characteristics of low erucic acid, low glucosinolate, high oleic acid, high linoleic acid, low arachidic acid and low protein content; a material with a GG type shows high erucic acid, high glucosinolate, low oleic acid, low linoleic acid, high arachidic acid and high protein content.The application can quickly screen out individuals with excellent quality traits in early generations, and avoids the long process of identifying traits after plants mature in traditional breeding.

This invention discloses a method for increasing the yield of arachidonic acid (RAA) produced by *Morchella spp.* fermentation, relating to microbial fermentation technology. The method employs either solid-state fermentation or liquid-state shallow static fermentation. *Morchella spp.*, used for RAA synthesis, is inoculated onto the surface of a solid-state fermentation medium for solid-state fermentation, or inoculated into a liquid fermentation medium for liquid-state shallow static fermentation. Feeding is performed during fermentation until the RAA content in the *Morchella spp.* reaches its peak. This method can increase the RAA content in synthesized oils to 1.72 times that of deep fermentation, reaching a maximum of 80.93%. The yield of RAA synthesized by *Morchella spp.* per 1L of culture medium consumed is up to four times that of deep fermentation. This method requires less equipment investment, is simple to operate, has very low energy consumption, is pollution-free, and allows for large-scale production of RA-rich oils and microorganisms, showing broad application prospects in pharmaceutical intermediates, nutritional supplements, and animal feed.

The application provides a thrombocytosis and thrombosis zebrafish model and a construction method and application thereof, and belongs to the technical field of biology. CALR del52 The transgenic zebrafish is obtained, and the transgenic zebrafish is added with a thrombus inducer of arachidonic acid to induce and construct; CALR del52 The nucleotide sequence is shown as SEQ ID NO. 1; the thrombocytosis and thrombosis disease zebrafish model provided by the application can be used for screening of anti-thrombus treatment drugs, has a short experimental cycle, a small dosage, and is favorable for high-throughput screening of trace anti-thrombus active natural products.

The application discloses a method for improving egg quality by using peony and a feed additive, and belongs to the field of animal husbandry. The method for improving egg quality comprises adding 0.75-1.5% of peony stem and leaf powder in the basic daily ration of laying hens. The method can significantly improve the production performance of laying hens in the later production stage, and the effect is better than that of aureomycin. Meanwhile, the method can also significantly enhance the antioxidation ability of the body, improve the enrichment of MUFA, gamma-linolenic acid and arachidonic acid in eggs, and has the potential to replace feed antibiotics.

This invention belongs to the field of green pest control technology in agriculture, and provides a method for controlling underground pests in peanuts: before crop sowing, previously planted Cnidium monnieri is pressed into the soil as green foliage; the sowing rate of Cnidium monnieri is 1-6.5 kg per mu, and the amount of green foliage returned to the soil is 7000-37500 kg / ha fresh weight, with the green foliage pressing occurring 5-10 days before crop sowing. The preferred underground pests are grubs, wireworms, and cutworms. This invention regulates the soil ecological environment through agronomic measures, which can increase the enrichment level of Metarhizium anisopliae in the soil and repel grubs, thereby reducing the occurrence of underground pests and mitigating yield losses caused by them. This method does not require the application of additional chemical agents specifically for underground pests and has good ecological and application value.

The invention discloses application of arachidonic acid in induction improvement of corn leukoderma resistance, the arachidonic acid is applied to prevention and treatment of corn leukoderma for the first time, and experiments show that the arachidonic acid can improve the photosynthetic effective leaf area, Fm content, Fv / Fm content, NPQ content, soluble sugar content and soluble protein content of plants; and / or increasing the activity of disease-resistant-related defensive substances; and / or catalyzing synthesis of lignin and phenolic substances; and / or the content of Rfd, MDA and reducing sugar of the plant is reduced to induce and improve the corn leukoderma resistance, so that a new direction and basis can be provided for preventing and treating the corn leukoderma by adopting natural compounds. It is found that the arachidonic acid does not strictly depend on the total spraying amount for inducing and improving the leukoderma resistance, but when the spraying amount is the same and the concentration of the arachidonic acid in the spraying liquid is 15 [mu] M, the arachidonic acid is optimal for inducing and improving the leukoderma resistance. And the application of arachidonic acid is beneficial to reducing the particle size of starch particles in corn kernels under the pressure of leukoderma.

The present application relates to a kind of genetic locus related to peanut leaf size, gene and molecular marker, belong to crop molecular genetics and molecular marker technical field.The two genetic loci related to leaf size of the present application are LA115165101 and LA115027188, wherein LA115165101 is located at the interval of 115165101-115165105 of the 5th chromosome of peanut, is base CGCTC or deletion;LA115027188 is located at 115027188 of the 5th chromosome of peanut, base is C or T;Two genes related to leaf size of the present application are Arahy.09KEBU and Arahy.PRU9CS, two functional KASP molecular markers are developed based on the gene, using the molecular marker can efficiently, accurately distinguish the leaf size trait of peanut, can effectively distinguish large leaf strain and small leaf strain in population, with the characteristics of simple operation, reliable result, low cost, suitable for large-scale molecular marker assisted selection, provide important technical support for cultivating ideal leaf shape, high yield and stable yield of new peanut variety.

The present application belongs to the field of biotechnology, and particularly relates to a method for improving accumulation of fucoxanthin, oleic acid and arachidonic acid in brown flagellate culture. The method is to add microcystis to brown flagellate for mixed culture, and continuously aerate, and the microcystis is phagocytosed by the brown flagellate. The method of phagocytosis of microcystis by brown flagellate is used to realize higher accumulation of fucoxanthin in brown flagellate. Through the experiment, it is proved that the brown flagellate has higher removal efficiency for non-toxic microcystis, and can also effectively ingest toxic microcystis, and a new way for obtaining higher accumulation of fucoxanthin in biological manufacturing is provided.

The application provides a method for promoting synchronous maturation of parent shrimps, which comprises the following steps: temporarily breeding male parent shrimps and female parent shrimps in a pool; adjusting water flow velocity to perform water flow domestication and temperature domestication, setting the water flow velocity to be 0.4-1.5 m / s, and feeding nutrition enhancer to perform intensive feeding when the water flow domestication is performed; the nutrition enhancer comprises the following components: taurine, arachidonic acid, phosphatidylserine and cuttlefish bone powder; setting the temperature to be 18-19 DEG C, increasing the temperature by 0.4-0.6 DEG C per day, and increasing the temperature to 27-28 DEG C. The water flow domestication and the temperature domestication are performed by adjusting the water flow velocity, and the intensive feeding of the nutrition enhancer is combined when the water flow domestication is performed, which is beneficial to the accumulation of nutritional substances in the hepatopancreas tissue of the female shrimps, promotes synchronous development and maturation of the ovary, and can make the maturation rate of the female shrimps reach 100%, and the synchronous maturation rate reach 80%.

The invention provides a preparation for resisting plant root rot and application, and belongs to the technical field of plant disease control. The compound lipid is prepared from docosanoic acid, arachidonic acid, oleic acid, gamma-linolenic acid and cis-11-eicosenoic acid. The compound lipid can inhibit fusarium oxysporum, and has a good effect in the aspect of preventing and treating plant root rot and plant fusarium wilt. The result of the embodiment shows that the growth condition of fusarium oxysporum in a culture medium can be obviously inhibited by adopting the compound lipid. The compound lipid is applied to prevention and treatment of Arabidopsis thaliana wilt and ginseng root rot, and the disease index of Arabidopsis thaliana wilt and ginseng root rot can be remarkably reduced. Therefore, the compound lipid provided by the invention has an efficient inhibition effect on the fusarium oxysporum as a phytopathogen, and can be applied to prevention and treatment of plant fusarium wilt and plant root rot.

The application belongs to the technical field of microbial application, and provides a strain of sphingomonas yanoikuyae with a preservation number of CGMCC No.28289.The strain can effectively degrade peanut phenolic acid allelochemicals, alleviate autotoxicity, and antagonize peanut soil-borne pathogenic bacteria, and can alleviate peanut continuous cropping obstacles.

This disclosure relates to a method for controlling or preventing plant pathogenic fungi on plants or their plant propagation material, the plant pathogenic fungi belonging to the genus *Cyclocarya paliurus*, *Bacillus oryzae*, *Cyclocarya argentea*, *Cyclocarya gleditsiae*, *Cyclocarya rotundifolia*, *Bacillus aubergine*, *Botrytis cinerea*, *Botrytis tulipta*, *Cercospora multiflora*, *Cercospora kikuchi*, and / or *Cercospora davidii*, the method comprising applying a composition containing jassamycin to the plant, its plant propagation material, or the site thereof.

The present utility model discloses the composition of sea grape (Caulerpa lentillifera) coconut cracker as a delicacy consisting of sea grapes, all-purpose flour, white sugar, powdered milk, grated mature coconut (Cocos nucifera), crushed fried peanut (Arachis hypogaea L.). and oil for frying. Based on the sensory evaluation, the sea grapes coconut cracker obtained ratings of “Like Extremely” in terms of color, aroma, texture and taste. Furthermore, results from the visual quality assessment confirmed that the product can be regarded as a delicious and nutritious delicacy suitable for all age groups.