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Industry method for producing arachidonic acid grease by using mortierella alpine

A technology of arachidonic acid and Mortierella alpina, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low mass percentage concentration and limited sources of arachidonic acid, and achieve the promotion of The ability to transform arachidonic acid, improve the dissolved oxygen level, and the effect of simple process

Inactive Publication Date: 2013-02-13
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Arachidonic acid mainly comes from animal liver, fish oil, bovine adrenal gland, etc., but the mass percentage concentration of arachidonic acid in animal tissues is very low, only 0.2%, and the source is very limited

Method used

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  • Industry method for producing arachidonic acid grease by using mortierella alpine
  • Industry method for producing arachidonic acid grease by using mortierella alpine
  • Industry method for producing arachidonic acid grease by using mortierella alpine

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Experimental program
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Effect test

Embodiment 1

[0031] 1. Activation of the original strain: inoculate the Mortierella alpina strain preserved in an amber tube on a PDA slant medium, cultivate it at 28±1°C for 8 days, select a PDA medium plate with vigorous growth of hyphae and spores, and remove the The hyphae and spores on the culture medium are made into spore suspension with sterile water;

[0032] 2. Seed culture: inoculate the spore suspension in the shake flask culture medium, shake and culture at 28±1°C for 48h, the shaking speed of the shaker is 200±20 rpm, the mass of the main raw material components in the liquid culture medium The percentage is glucose 4%, peptone 1%, yeast extract 3%, matrix distilled water, pH value is 7.2;

[0033] 3 seed expansion cultivation: the final fermenter volume is 50m 3 , so the selected seed pots are 10m 3 、1m 3 , inoculate 1.5L of shake flask seed fermentation liquid in step (2) to 1m 3 In the seed expansion fermenter, the mass percentages of the main raw material components i...

Embodiment 2

[0062] 1. Activation of the original strain: inoculate the Mortierella alpina strain preserved in an amber tube on a PDA slant medium, culture it at 28±1°C for 9 days, select a PDA medium plate with vigorous growth of hyphae and spores, and remove it. The hyphae and spores on the culture medium are made into spore suspension with sterile water;

[0063] 2. Seed culture: inoculate the spore suspension in the shake flask culture medium, shake and culture at 28±1°C for 48h, the shaking speed of the shaker is 200±20 rpm, the mass of the main raw material components in the liquid culture medium The percentage is glucose 5%, peptone 1.5%, yeast extract 2%, matrix distilled water, pH value is 7.0;

[0064] 3 seed expansion cultivation: the final fermenter volume is 200m 3 , so the seed tanks are 50m respectively 3 、5m 3 , 500L, inoculate 1L shake flask seed fermentation liquid in the seed expansion fermenter of 500L, cultivate 24h under the temperature of 30 ± 2 ℃, the bacterium c...

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Abstract

The present invention provides an industry method for producing arachidonic acid grease by using mortierella alpine. The method is characterized by comprising the following steps: activating an original strain; carrying out seed culture; carrying out seed enlarge culture; and carrying out fermentation culture, wherein the fermentation culture comprises an initial fermentation stage, the seed enlarge culture liquid is inoculated in a fermentation tank to carry out stirring culture, glucose and edible oil are supplemented according to the situation, the initial fermentation stage is completed when bacterial concentration of the fermentation broth achieves 40% (by ??volume) and the dried mycelium total oil achieves 40 wt%, a post-fermentation stage starts, the fermentation broth is cooled to a temperature of 23-27 DEG C, sterile ammonia water is concurrently added to adjust the pH value to 8-9, standing fermentation is performed while the glucose content is controlled, the post-fermentation stage is completed when the arachidonic acid content is higher than 45 wt%, and the obtained fermentation broth is subjected to a fermentation treatment to obtain the grease containing the arachidonic acid. The industry method has the following characteristics that: a fermentation formula is simplified, specific yield of the arachidonic acid can be 10 gARA / L fermentation broth through the specific process control, a process is simple and easy to control, and industrial production is achieved.

Description

technical field [0001] The invention relates to a method for industrialized production of arachidonic acid, in particular to an industrial method for producing arachidonic acid oil by utilizing Mortierella alpina expansion under specific process conditions. Background technique [0002] Arachidonic Acid (ARA) is an n-6 series polyunsaturated fatty acid, which is a precursor for the synthesis of human prostaglandins, thromboxanes and leukotrienes, and has a wide range of biological activities. Arachidonic acid also has an important nutritional function. As a natural component of human breast milk, it is essential for the baby's neurological and physiological development. It has been recommended by many expert organizations including the World Health Organization as a nutritional supplement to add to infant formula milk powder middle. Arachidonic acid is mainly derived from animal liver, fish oil, bovine adrenal gland, etc., but the mass percentage concentration of arachidoni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
Inventor 唐孝鹏汪志明张玉良朱子清
Owner CABIO BIOTECH WUHAN CO LTD
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