179 results about "Arachidonic acid" patented technology

The invention relates to the technical field of feed products, in particular to a fatty acid composition and application thereof in preparation of products for improving intestinal health. The fatty acid composition comprises arachidonic acid, docosahexaenoic acid, eicosapentaenoic acid, butyric acid and lauric acid, or a precursor substance capable of releasing the fatty acid in the digestive tract, and the mass of the fatty acid composition is calculated according to the mass of the arachidonic acid, the mass of the docosahexaenoic acid, the mass of the eicosapentaenoic acid, the mass of the butyric acid and the mass of the lauric acid. The additive is prepared from the following components in parts by weight: 7-10 parts of arachidonic acid, 3-7 parts of docosahexaenoic acid, 3-7 parts of eicosapentaenoic acid, 2-5 parts of butyric acid and 1-3 parts of lauric acid. The composition can effectively relieve inflammation and diarrhea, regulate intestinal flora and improve intestinal health, and drug resistance is not prone to being generated.

The invention discloses a preparation method of a bio-based epoxy resin anticorrosive coating, and relates to a preparation method of a bio-based epoxy resin coating. The bio-based epoxy resin anticorrosive paint mainly comprises an anticorrosive filler COFs-AA, bio-based epoxy resin and a curing agent. Wherein the anticorrosive filler COFs-AA is prepared from a two-dimensional covalent organic framework COFs and a bio-based material arachidonic acid (AA) through a covalent condensation reaction; the bio-based epoxy resin is prepared by epoxidizing a bio-based material galangin serving as a raw material through epichlorohydrin, and the curing agent is a common curing agent for epoxy resin. The bio-based epoxy resin anticorrosive paint is compounded by taking biomass as a basic component, and has the characteristics of greenness, environmental protection, no toxicity, good hydrophobicity and good corrosion resistance; the bio-based epoxy resin anticorrosive paint can be applied to the field of corrosion protection of materials such as various metals and woodware.

The invention provides universal functional formula milk powder for cats and dogs and a preparation method thereof, and relates to the field of pet formula food. The formula milk powder consists of goat milk powder, colostrum powder, whey protein powder, lactoferrin powder, yolk powder, immune globulin powder, compound grease, compound minerals, compound vitamins, choline, taurine, compound prebiotics, compound probiotics, docosahexaenoic acid, arachidonic acid, casein phosphopeptides, L-glutamine and beta-galactosidase. And the goat milk powder, colostrum powder, whey protein powder, lactoferrin powder, yolk powder and immune globulin powder are subjected to low-temperature sterilization treatment by adopting an induced electric field or high-pressure microjet. The cat and dog formula milk powder overcomes the defects in the prior art, through compounding of basic nutritional ingredients and functional ingredients, a novel low-temperature sterilization technology is adopted for sterilization treatment, on the premise that the functionality of the formula milk powder is guaranteed, the nutritional value of the formula milk powder is reserved to a greater extent, and healthy growth and development of cats and dogs are promoted.

The invention provides a group of metabolic markers, a kit and application of the metabolic markers in auxiliary diagnosis of schizophrenia. The metabolic markers comprise arachidonic acid, palmitamide and lysophosphatidylcholine (LPC) (17: 0 / 0: 0). By utilizing the metabolic marker, auxiliary diagnosis of schizophrenia can be rapidly and accurately completed in the early stage of disease attack.

The invention belongs to the technical field of animal molecular markers, and particularly relates to an SNP molecular marker for beef cattle flavor character breeding and application of the SNP molecular marker. The invention proposes that genotype polymorphism of 84181318bp and 84181699bp on chromosome 3 of an international bovine reference genome AR-UCD1.2 version is related to the character of the content of the beef arachidonic acid for the first time, the genotype polymorphism can be used for evaluating the content of the beef arachidonic acid, and beef cattle with specific genotypes at the two sites has relatively high content of the beef arachidonic acid. The site can be used as a molecular marker for evaluating the content of the arachidonic acid in the beef cattle, early prediction and auxiliary screening of beef cattle individuals with excellent meat quality and flavor characters, and a theoretical basis and a technical support are provided for breeding of the beef cattle with high content of the arachidonic acid in the beef cattle.

The invention discloses a KASP molecular marker related to brassica napus quality traits and application of the KASP molecular marker, and belongs to the technical field of molecular genetics. According to the KASP molecular marker related to the brassica napus quality traits, the primers of the KASP molecular marker and the application of the KASP molecular marker and the primers in brassica napus genetic typing or quality traits identification, results show that the KASP molecular marker can accurately identify genotypes, is high in sensitivity and specificity and effectively reduces the error rate. The invention also discloses a brassica napus genotyping / quality identification method, and the result shows that the material with the genotyping result of AA has the characteristics of low erucic acid content, low thioglycoside content, high oleic acid content, high linoleic acid content, low arachidonic acid content and low protein content; gG-type materials exhibit high erucic acid, high thioglycoside, low oleic acid, low linoleic acid, high arachidonic acid and high protein content. Individuals with excellent quality characters can be rapidly screened in early generations, and the long process of character identification after plant maturation in traditional breeding is avoided.

The invention relates to a metabolic biomarker for predicting the cardiovascular disease risk of a patient with senile subclinical hypothyroidism and application of the metabolic biomarker. The marker comprises inosine, arachidonic acid, arginine, phenylalanine and glycocholic acid. According to the method, metabolite information of each plasma sample in a research queue is obtained by establishing an elderly SCH and concurrent CVD crowd queue and detecting by adopting a high performance liquid chromatography-mass spectrometry technology, and a targeted metabolic content map is established. According to the matrix expression of the metabolic marker, in combination with clinical follow-up visit data, a model is constructed by using machine learning Lasso-cox, and a clinical prediction model of the CVD risk of the elderly SCH patient is constructed to guide precise diagnosis and treatment of the elderly SCH. The invention further provides a kit containing the diagnostic marker suitable for predicting the CVD risk of the elderly SCH, and the kit can be used for predicting the CVD risk of the elderly SCH.

The invention relates to the technical field of cell culture. The invention provides a mesenchymal stem cell culture medium without animal-derived components and application of the mesenchymal stem cell culture medium. The culture medium comprises a basic culture medium and an additive, the additives comprise FGF-2, IGF-1, glutamine, folic acid, serotonin, cholesterol, arachidonic acid, an HEPES buffer solution, sodium bicarbonate, SCF, TGF-beta, PDGF, ascorbic acid and transferrin. The mesenchymal stem cell culture medium disclosed by the invention is completely free of animal-derived components, and meets the requirements of FDA / EMA clinical-grade cell therapy products. The culture medium can support high-density amplification of mesenchymal stem cells, the activity of passage cells is effectively improved, and the amplification time is shortened. The method has remarkable significance in improving the amplification efficiency of the mesenchymal stem cells and promoting and applying factory growth.

The present invention provides special medical use formulae useful for enhancing and / or improving the social behavior of an individual. The formula food for the special medical purpose comprises DHA (docosahexaenoic acid), ARA (arachidonic acid) and breast milk oligosaccharide 6-SL and / or LNnT, and the mass ratio of the DHA to the ARA to the breast milk oligosaccharide 6-SL and / or LNnT is (70-210): (60-420): (10-400). The special medical use formulae of the present invention contribute to enhancing and / or improving the social behavior of an individual.

The invention relates to the field of biological medicine, and discloses application of LPC, PC and AA in children sepsis diagnosis and prognosis product development. According to the invention, a high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS / MS) technology is used, plasma samples of a child sepsis patient and a healthy control group are compared, the relationship between lipidome change and pSOFA score is analyzed, and specific lipid molecules closely related to disease severity and prognosis are identified. According to the invention, the following lipid molecules are highlighted as key biomarkers: lysophosphatidylcholine (LPC), phosphatidylcholine (PC) and arachidonic acid (AA). The change of the lipid molecules is closely related to the physiological state of a child sepsis patient, and the lipid molecules can be used for early diagnosis and evaluation of the state of the child sepsis, so that the treatment effect and the accuracy of prognosis evaluation are improved, and a new treatment strategy and a new management method are provided for clinic.

The present invention belongs to the technical field of nutritional products, and specifically relates to a neuraminic acid composition for improving memory and learning functions and a preparation method thereof. The present invention has developed a multi-component and multi-functional neuraminic acid composition, in which the various nutrients contained therein synergistically enhance the effect of improving memory and learning functions. By adding lysolecithin and resistant dextrin during the preparation of the neuraminic acid composition, a variety of functional components with poor stability and easy oxidation contained in the composition can be stabilized, including neuraminic acid, DHA, arachidonic acid, etc., so that the obtained neuraminic acid composition has a longer shelf life and also improves the bioavailability of the functional components in the composition.

Lymphedema is characterized by the accumulation of protein-rich interstitial fluid, lipids and a significant inflammatory cell infiltrate in the limb. It causes a significant morbidity and is a common disabling disease affecting more than 250 million people worldwide, however there is no curative treatment for lymphedema. Here, the inventors found that dermolipectomies from patient with lymphedema exhibit inflamed gene expression profile compared to normal arm on same patient. After lipidomic analysis, the inventors identified severe decrease in arachidonic acid-derived lipid mediators generated by the 15-lipoxygenase (15-LOX) in lymphedematous arms. Using a mouse model of lymphedema, they reproduced the etiology of the human pathology including the loss of specialized pro-resolving lipid mediators that play essential roles in resolution of inflammation. This was associated with a lack of regulatory T cells (Treg) recruitment in the injured limb adipose tissue. Importantly, the inventors identified the lymphatic endothelial 15-LOX was responsible for the chemoattraction and transendothelial migration of Tregs. These results were confirmed by an aggravation of lymphedema and deterioration of the lymphatic network in an original transgenic mouse model in which ALOX15 gene is selectively deleted in the lymphatic system. Importantly, this was reversed by the injection of 15-LOX expressing lentivectors. These results provide evidence that lymphatic lipoxygenase may represent a novel therapeutic target for lymphedema by serving as a mediator of Treg invasion into lymphedematous adipose tissue.

An Alzheimer's disease biomarker based on a brain metabolite and a use thereof. The biomarker comprises any one or a combination of at least two of palmitic acid, DHA, gallic acid, 11Z,14Z,17Z-eicosatrienoic acid, glycodeoxycholic acid, palmitoleic acid, linoleic acid, erucic acid, petroselinic acid, and arachidonic acid. The level of a metabolite is detected to assist in early diagnosis of the Alzheimer's disease, thus facilitating rapid detection; in addition, the present invention has the characteristics of timeliness, convenience, high specificity and high sensitivity.

Disclosed herein are compositions and methods for controlling chronic inflammation in a patient. The compositions and methods employ D6-arachidonic acid or an ester thereof (D6-AA) as an anti-inflammatory agent that provides for significant reduction in inflammatory processes. In addition. The compositions of deuterated arachidonic acid or ester thereof comprise, on average, at least about 80% of the hydrogen atoms at each of the bis-allylic sites having been replaced by deuterium atoms and, on average, no more than about 30% of the hydrogen atoms at the mono-allylic sites having been replaced by deuterium atoms.

The invention discloses a positive ion saliva marker related to brain glioma and meningioma and application of the positive ion saliva marker, and relates to the field of biological medicine. The positive ion saliva marker is prepared from one or more of L-arabitol, 20-hydroxyarachidonic acid, sulfonate and palm oil acylethanolamide. The invention provides a kit which is used for detecting positive ion saliva markers related to brain glioma and meningioma and comprises a specific detection reagent. Besides, the invention further provides a computer program product which can judge whether the patient to be detected is a glioma patient or a meningioma patient, so that a solid foundation is laid for subsequent treatment. The method has good feasibility and accuracy, is safe and noninvasive, can effectively distinguish brain glioma and meningioma, and provides a brand new powerful tool for clinical diagnosis.

The invention provides a pulmonary nodule targeting food and medicine homologous composition based on metabonomics screening and application thereof. By analyzing a pulmonary nodule specific metabolic profile, active components, namely platycodin D, mogroside V, oyster active peptide and fermented cordycepin, which can cooperatively regulate and control an arachidonic acid inflammation pathway, a glutathione antioxidant pathway and a tricarboxylic acid cycle energy pathway are reversely screened out. Animal experiments prove that the composition can reduce the volume of pulmonary nodules by 58.3%, human body application shows that the nodules of 76.7% of subjects are reduced, and liver and kidney toxicity is avoided. The composition can be prepared into tablets, capsules and other dosage forms, can be used as functional food or adjuvant therapy drugs for high risk groups of pulmonary nodules, and realizes accurate nutrition intervention.

The invention discloses a compound grease feed additive for improving breeding quality of channa maculata and application. The method comprises the following steps: (1) preparing experimental feed; the method comprises the following steps: respectively preparing an experimental group ARA 1.5 added with 1.5% of arachidonic acid and an experimental group ARA + PC added with 1.5% of arachidonic acid and 1.5% of phosphatidylcholine; (2) feeding; (3) after the culture experiment is finished, treating and detecting the channa maculata; (4) carrying out an artificial propagation test; (5) carrying out ovarian steroid synthesis related gene expression detection; and (6) carrying out data statistical analysis. By adding arachidonic acid (ARA) and phosphatidylcholine (PC) into the feed, the breeding performance of channa maculata and the quality of fry are improved, and the feed has very important significance for improving the aquatic product efficiency of high-quality fry of channa maculata in aquaculture.

The application relates to a probiotic / arachidonic acid composite microcapsule and a preparation method thereof, and belongs to the technical field of microcapsule preparation, and the specific scheme is as follows: a probiotic / arachidonic acid composite microcapsule comprises wall material and core material, the core material is wrapped in the wall material, the wall material comprises sodium alginate and sodium carboxymethyl cellulose, the core material comprises arachidonic acid and probiotics, the arachidonic acid is dissolved in an oily carrier and forms a water-in-oil emulsion with a probiotic bacterial suspension, the sodium carboxymethyl cellulose wraps water-in-oil emulsion droplets, and the sodium alginate reacts with the sodium carboxymethyl cellulose to generate a gel film microcapsule wall material. The method for preparing the probiotic / arachidonic acid composite microcapsule provided by the application is simple to operate, the microfluidic chip is simple to prepare and low in cost, has a high embedding rate, the particle size of the microcapsule is controllable, and the biological activity is good.

The application belongs to the technical field of molecular biology, and particularly relates to metabolic markers for evaluating the prognosis of patients with hypertrophic cardiomyopathy and application thereof. The application determines that one or more of 3-hydroxyrilotene, asparagine-lysine, cyclohexylamine, arachidonic acid, aconitic acid, propionyl carnitine, ceramide, tryptophan, 3-hydroxysebacic acid, traumatic acid, phosphatidylcholine, alanine-proline, lysophosphatidylcholine, taurine and S-garlicin cysteine are related to the prognosis of hypertrophic cardiomyopathy, can evaluate the prognosis of patients with hypertrophic cardiomyopathy after undergoing muscle resection of interventricular septum, and shows accurate judgment on all-cause death or rehospitalization, cardiovascular events and postoperative new-onset arrhythmia. Finally, all the prognostic biomarkers are reproduced in an independent cohort, which is helpful for postoperative risk evaluation of patients with hypertrophic cardiomyopathy, has high specificity and high sensitivity.

The application belongs to the technical field of biotechnology, and particularly relates to a kind of snake venom polypeptide and application thereof.The snake venom polypeptide is polypeptide DACU-1, polypeptide DACU-2 or polypeptide DACU-3.The application provides a kind of new snake venom polypeptide, including three polypeptides, and the three polypeptides all have anti-platelet aggregation activity, and have the activity of inhibiting platelet aggregation under the stimulation of ADP, arachidonic acid, collagen, thrombin and other various activators, and have the potential to be developed into anti-platelet drugs, functional foods and health products.

The present invention belongs to the technical field of genetic engineering, and particularly relates to an GmHMGS application of a gene and a recombinant overexpression vector in regulating the fatty acid content of plants, and the GmHMGS nucleotide sequence of the gene is shown as SEQ ID NO.1. The present invention uses an overexpression vector of an exogenous gene sequence to introduce the GmHMGS gene shown as SEQ ID NO.1 into plants for overexpression GmHMGS of the gene, and the plants show that the contents of palmitoleic acid, arachidonic acid, docosanoic acid and erucic acid are significantly higher than those of the wild type; at the same time, the contents of stearic acid, oleic acid and linolenic acid are significantly reduced. The present invention regulates the GmHMGS expression of the gene in plants and then uses it to cultivate new plant varieties with adjustable fatty acid content, which provides a new idea for cultivating new crop varieties and has great application value in molecular breeding.

A method for inhibiting the progression of amyotrophic lateral sclerosis (ALS) in humans is disclosed that utilizes a synergistic combination of 2,4-dihydro-3H-pyrazol-3-one and deuterated arachidonic acid or a prodrug thereof, such as 11,11-D2-linoleic acid or an ester thereof.

The application discloses application of Fagopyrum cymosum root powder in preparation of products for improving egg quality or intestinal health of laying ducks, and belongs to the technical field of feed additives. The application provides application of Fagopyrum cymosum root powder in preparation of products for improving egg quality or intestinal health of laying ducks, and the Fagopyrum cymosum root powder can improve eggshell strength, yolk color and eggshell proportion, improve the content of aspartic acid, threonine, glutamic acid, glycine, cystine and leucine in yolk, improve the content of arachidonic acid and arachidonic acid in yolk, and reduce the content of saturated fatty acid in yolk; the Fagopyrum cymosum root powder can improve the activity of maltase in the mucous membrane of the jejunum of laying ducks, and can improve the diversity and abundance of intestinal flora of laying ducks. The application provides a new strategy for improving egg quality or intestinal health of laying ducks.

The invention discloses application of a metabolite of arachidonic acid in mycoplasma pneumonia, and relates to the technical field of biological detection. According to the application of the arachidonic acid metabolite in mycoplasma pneumonia, the arachidonic acid metabolite is detected, so that the content of the arachidonic acid metabolite in a biological sample is accurately detected through chromatography-mass spectrometry according to the change of the level of the metabolite in inflammatory reaction, and the arachidonic acid metabolite can be used as an auxiliary index for diagnosis of inflammatory diseases such as pneumonia; if in a pneumonia patient, the concentration may be higher than that of a healthy person; in different types of inflammatory diseases, metabolite change modes of arachidonic acid are different, and detection of the metabolite in combination with other clinical indexes is helpful for distinguishing pneumonia and other inflammatory diseases with similar symptoms, such as differential diagnosis of bronchitis and the like; meanwhile, in the pneumonia development process, the inflammation progress can be known by detecting the content of metabolites.

The invention discloses a composite elicitor for promoting taxus chinensis embryogenic stem cells to produce paclitaxel and application, and belongs to the technical field of biology. The compound elicitor consists of methyl jasmonate, indolebutyric acid and salicylic acid, and the working concentrations of the methyl jasmonate, the indolebutyric acid and the salicylic acid in a culture medium are respectively as follows: 50-800 mu mol / L of methyl jasmonate, 12.5-50 mu mol / L of indolebutyric acid and 0.1-0.5 mg / L of salicylic acid. Preferably, the jasmonic acid methyl ester is 200 [mu] mol / L, the indolebutyric acid is 25 [mu] mol / L, the salicylic acid is 0.5 mg / L, and 0.1-0.5 mg / L arachidonic acid can be selectively added. The composite elicitor is added in the early stage of the exponential growth phase of the taxus chinensis embryogenic stem cells, and the taxus chinensis embryogenic stem cells are continuously cultured for 5 days, so that the yield of paclitaxel reaches 27.52 mg / L and is 7.86 times that of blank control, and meanwhile, the maximum biomass is obtained. According to the invention, the synergistic interaction of the three elicitors is realized for the first time, the production period is remarkably shortened, a set of efficient and stable paclitaxel production system is established, and an innovative solution is provided for large-scale production of paclitaxel.

The invention discloses a preparation method of an arachidonic acid lipid nano drug delivery system, and relates to the technical field of biology, the lipid nano drug delivery system comprises the following components: (1) ionizable lipid; (2) arachidonic acid; (3) a conjugated lipid that inhibits particle aggregation of a non-cationic lipid or an ionizable lipid; (4) non-cationic lipids or non-ionizable lipids other than (3); (5) metal ions; (6) a nucleic acid drug; the lipid nano drug delivery system is formed by mixing and self-assembling at least the components (1) to (6). According to the novel preparation method of the lipid nano drug delivery system, cancer cells are specifically killed through lipid replacement and simultaneous use of nuclear nucleic acid drugs and metal ions, the treatment accuracy is improved, and the stability and the lysosome escape rate can be increased.

The present invention discloses a breeding method for sturgeon, a sturgeon feed and a preparation method thereof, belonging to the technical field of aquaculture. The sturgeon feed includes basic nutrients such as protein, fat, vitamins and minerals. In the feed, arachidonic acid accounts for 0.1% - 2% of the weight percentage of the feed. In addition, the present invention also provides a preparation method for the above sturgeon feed, including the following steps: S1. Mix fish oil, soybean oil, choline, arachidonic acid, compound multivitamins, compound multiminerals, first flour and water, and granulate to obtain the first granules; S2. Mix the first granules, fish meal, soybean meal, chicken meal, squid visceral powder, sodium carboxymethylcellulose, calcium dihydrogen phosphate and second flour, and granulate and dry to obtain the second granules. In addition, the present invention also provides a breeding method for sturgeon. The feed provided by the present invention can develop the ovaries of 10-year-old Yangtze sturgeon at stage III to stage IV when fed.

The invention provides application of arachidonic acid in delaying reproductive aging, and particularly relates to the technical field of reproductive health. The invention provides application of arachidonic acid in preparation of a product for delaying reproductive aging and / or prolonging the reproductive period. By taking caenorhabditis elegans and mice as examples, the arachidonic acid can obviously activate reproductive aging gonad lysosome, protect oocyte quality and prolong reproductive cycle, thereby delaying reproductive aging. The invention provides a new application prospect of arachidonic acid in the aspects of delaying ovarian aging and protecting fertility.

The invention belongs to the technical field of functional food, and particularly relates to a brain-strengthening and eyesight-improving composition as well as a preparation method, a product and application thereof. The composition is prepared from algal oil, linseed oil, arachidonic acid, marigold extract, zeaxanthin suspension oil, NA nervonic acid and PS phosphatidylserine according to a specific weight part ratio. Wherein the marigold extract is prepared by adopting freeze-grinding pretreatment, combining enzymolysis and an ultrasonic-microwave synergistic extraction technology, and performing nanofiltration membrane concentration and vacuum freeze-drying. According to the composition, through the synergistic effect of all the components and an innovative extraction process, the effects of efficiently strengthening the brain and improving eyesight are achieved, and compared with a traditional formula and an extraction method, the component activity retention and the effective component extraction rate are remarkably improved.

The invention provides a feed additive for improving the hypoxia tolerance function of micropterus salmoides. The feed additive is prepared from the following raw materials in percentage by mass: 20% of arachidonic acid and the balance of maltodextrin. The invention also provides a feed composition of the feed additive. The feed is prepared from the following raw materials in percentage by mass: 450 per mill of fish meal, 50 per mill of fermented hermetia illucens pulp, 69.5 per mill of enzymolysis chicken powder, 60 per mill of chicken liver powder, 60 per mill of enzymolysis soybean meal, 75 per mill of fermented soybean meal, 60 per mill of cottonseed meal, 29 per mill of dextrin, 40.5 per mill of cassava starch, 23 per mill of soybean oil, 23 per mill of linseed oil, 15 per mill of monocalcium phosphate, 5 per mill of choline chloride, 1 per mill of a micropterus salmoides premix, 1 per mill of zeolite powder and 20 per mill of a feed additive. The feed composition is used for improving the hypoxia tolerance of the micropterus salmoides. According to the invention, arachidonic acid is used as a functional active substance to prepare the hypoxia stress resistant functional feed, and the addition of arachidonic acid can significantly activate liver angiogenesis genes, promote the liver angiogenesis level and improve the hypoxia tolerance of largemouth bass.