56 results about "Lactulose" patented technology

Prebiotic formulations can modulate the growth and / or activity of microorganisms in the urogenital area. Such prebiotic formulations can comprise isomaltulose along with at least one other prebiotic, such as 1-kestose or lactulose. These microorganisms which are modulated can be directly related to urogenital health, and their modulation can be used for the prevention, control, or treatment of dysbiosis of the urogenital area.

The invention discloses a cellobiose 2-epimerase mutant as well as a gene, an expression vector, recombinant bacteria, a preparation method and application thereof. The mutant is obtained by performing the following point mutation on an amino acid sequence as shown in SEQ ID No.1: S12A, S24A, G131A, G176A, G224A, K46R, K69R, K72R, K91R, K103R, K201R, K219R, K223R, K241R, K263R, K266R, K273R, K284R, K325R, K335R, K349R and K387R. The invention further discloses a preparation method of the mutant. The invention further discloses a gene segment, a plasmid and a recombinant bacterium for recombinant expression of the mutant, and the gene segment, the plasmid and the recombinant bacterium are used for recombinant expression preparation of the mutant. The mutant can be used for continuously and stably catalyzing and preparing lactulose at high temperature, is relatively good in thermal stability and has a relatively good industrial application prospect.

1. Name of the designed product: packing box (milk fruit sugar oral solution). 2. Use of the designed product: for product packing. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo showing the design points: perspective view 1.

This invention discloses a pet food with coat-enhancing, shine-promoting, and coat-protecting effects, its preparation method, and applications. The food comprises the following components: chicken meal, fresh chicken meat, duck meal, sardine meal, chicken liver, sweet potato meal, corn meal, chicken heart, beef bone meal, duck meat pear, chicken oil, and egg yolk granules; coriander root powder, inulin, seaweed powder, beet pulp, flaxseed, fish oil, cranberry powder, carrot, krill, honeysuckle, turmeric, chrysanthemum, mannan oligosaccharide, lactulose, etc. The raw materials and auxiliary ingredients of this pet food are widely available, palatable, inexpensive, and readily available. The coriander root powder, other auxiliary ingredients, and nutritional supplements / additives work synergistically to improve the shine of the pet's coat, promote growth, and maintain coat health, while also improving the microstructure of the hair cuticle, fundamentally preventing pet coat health problems. The pet food preparation method disclosed in this invention is simple to operate, safe, and environmentally friendly, and is particularly suitable for feed products with high humidity and high heat sensitivity.

The invention provides novel injection type glycerine enema which comprises an injection assembly, and the injection assembly comprises an injection tube, a piston, a push rod, a push block, a nipple, a fixing ring, a sealing ring, a gradually-hardened tube, a cavity, an L-shaped plate, a clamping block, a flexible steel rope, a pull ring, a mounting block and a clamping groove. And a piston is connected to the inner wall of the injection tube in a sliding manner. The gradually-hard tube is inserted into a place where the injection tube is inserted, the push block is pushed to drive the push rod to move, the push rod drives the piston to slide on the injection tube, and liquid medicine is pushed in through the gradually-hard tube, outflow and injection caused by two-time extrusion of common glycerine enema are avoided through one-time injection of 20 ml, two-time mucosa damage is avoided, pollution caused by excrement back-pumping after extrusion is finished is avoided, and the injection efficiency is improved. The pull ring is pulled leftwards, the pull ring drives the clamping block to be separated from the clamping groove through the flexible steel rope, the sealing ring is moved to take down the gradually-hard tube, and the gradually-hard tube is disinfected and cleaned respectively, so that the comfort degree is improved, the rectal mucosa is not prone to being damaged, all liquid medicine can be pushed in in a pushing injection mode, and the liquid medicine is not left and wasted.

The problem addressed by this invention is to provide a method for producing a solid molded body excellent in transportation aptitude and solubility, and use thereof. Said problem is solved by a method for producing a solid molded body, the method comprising: a granulation step for granulating a composition containing a protein-containing food (a) and a carbohydrate (b); and a molding step for molding a granulated product obtained in the granulation step, wherein the amount of the carbohydrate (b) is 1-50 mass% with respect to the total amount of the composition, and the carbohydrate (b) contains one or more selected from the group consisting of maltose, erythritol, trehalose, glucose, lactose, lactulose, and dextrin.

The invention provides a gene expression cassette for improving CsCE expression quantity and application. The gene expression cassette provided by the invention comprises a regulatory element and a target gene, the regulatory element comprises at least one of a coding sequence of dtRNA, a coding sequence of a signal peptide and a coding sequence of a fusion tag, and the target gene comprises a coding sequence of CsCE enzyme. Wherein the dtRNA contributes to keeping the structural stability when the CsCE enzyme is transcribed into mRNA, the signal peptide can promote the secretion of the CsCE enzyme, and the fusion tag can assist the correct folding of the CsCE enzyme, so that the expression of the CsCE enzyme can be synergistically regulated under the three-tube alignment of a DNA transcriptional mRNA layer, a protein secretion layer and a protein folding layer, the expression quantity of the CsCE enzyme can be effectively up-regulated, and the expression quantity of the CsCE enzyme can be effectively up-regulated. Therefore, the volume enzyme activity of the CsCE enzyme is improved, and the CsCE enzyme with higher volume enzyme activity can catalyze lactose to be converted into lactulose in the preparation of lactulose, so that the preparation yield of lactulose is improved.

The invention provides a method for separating lactulose and lactose by using a sequential simulated moving bed chromatography system, and relates to the technical field of saccharide separation and purification. According to the invention, the sequential simulated moving bed chromatography system is used for separating and purifying lactulose, and the analysis area, the refining area, the adsorption area and the isolation area can be operated at the same time under the set temperature condition, so that the separation and purification efficiency of lactulose is remarkably improved, continuous large-scale separation and purification of lactulose can be realized, the separation and purification effect is good, the yield is high, and the product quality is good. According to the method, pure water is used as an eluent, so that analyzed and purified compounds are free of damage and toxicity, rich in source and low in cost.

This invention discloses a cellobiose epimerase mutant, engineered bacteria, and its applications. The mutant is obtained by mutating leucine at position 227 of the cellobiose epimerase to valine. The nucleotide sequence of the cellobiose epimerase is shown in SEQ ID NO.1. This invention involves site-directed mutagenesis of existing cellobiose epimerases, maximizing the catalytic performance of the natural enzyme. The resulting mutant exhibits a high lactulose conversion rate. Under optimized conditions, using crude enzyme solution from recombinant bacteria as a catalyst and lactose as a substrate, the conversion rate from lactose to lactulose reaches 70%–73.3%, while the yield of ipilactose is only 7–10%, lower than the byproduct ratio allowed by the Chinese Pharmacopoeia. This demonstrates the potential for large-scale application and has extremely high application value in the industrial production of lactulose.

The application discloses a cellobiose epimerase mutant with high lactulose yield and application thereof, and belongs to the technical field of enzyme engineering. Dictyoglomus The cellobiose epimerase (referred to as Disp-WT enzyme) derived from microorganism Bifidobacterium longum sp. is used as a parent, a gene mutation technology is used, a histidine His at the 245th position is replaced by glutamic acid Glu, and a mutant H245E is obtained.The optimal catalytic condition of the mutant enzyme H245E does not change, but the isomerase activity of the enzyme at 85 DEG C is increased to 254 % of the original, and the generation amount of epi-lactose is greatly reduced.This finding has important research value for researching more cellobiose epimerases with low isomerase activity.

The invention discloses a fermented camel yogurt beverage and a preparation method thereof, and relates to the technical field of fermented dairy products. According to the invention, lactulose is cooperated with a fermentation strain to realize in-vivo and in-vitro dual probiotic synergy, Kluyveromyces marxianus is cooperated with fruit juice to generate natural fragrance, a flavor-guided fermentation technology and a two-stage temperature-controlled fermentation technology are applied, and an ultra-high pressure homogenization technology is combined, so that the flavor-guided fruit juice is obtained. Finally, the fermented camel yogurt beverage which is fine and stable in texture, natural and coordinated in flavor, outstanding in probiotic function, long in shelf life and excellent in weather resistance is obtained.

The invention belongs to the technical field of food, and particularly relates to a preparation method of crystalline lactulose. The preparation method of the crystalline lactulose comprises the following steps: (1) weighing lactulose powder, dissolving in water, heating and stirring to obtain a lactulose solution; (2) adding a composite regulating agent into the lactulose solution obtained in the step (1), and monitoring the viscosity to obtain a mixed solution; (3) adding lactulose crystal seeds into the mixed solution obtained in the step (2) for induced crystallization; then gradient cooling crystallization is adopted; (4) after crystallization is completed, carrying out heat preservation, separation, washing and drying to obtain a lactulose crystal product, and collecting mother liquor for cyclic utilization; according to the preparation method of the crystalline lactulose, the viscosity of the mother liquor can be reduced, the product purity is high, and the yield is high.

The invention belongs to the technical field of chromatographic separation, and particularly relates to a chromatographic column combined lactulose separation method which comprises the following steps: A1, catalytically converting lactose into lactulose to obtain a lactulose conversion solution; a2, adjusting the pH value of the lactulose conversion liquid, performing nanofiltration to obtain a clear liquid and wastewater, performing ion exchange on the clear liquid, and concentrating to obtain a feed liquid; a3, conveying the feed liquid into a first-stage chromatographic separation system, and carrying out first-stage washing separation to obtain a first-stage product containing more than 94 wt% of lactulose and a recovery liquid; and A4, carrying out decoloration, ion exchange and evaporation concentration on the primary product, conveying the primary product into a secondary chromatographic separation system, carrying out secondary washing separation to obtain a secondary product containing more than 99 wt% of lactulose and a waste liquid, and carrying out fixed bed ion exchange, decoloration and evaporation concentration on the secondary product to obtain a lactulose product. According to the method, 99 wt.% of lactulose products are obtained, and a green and environment-friendly process route is opened up for industrial preparation of high-purity lactulose.

The invention discloses a cellobiose epimerase mutant with improved enzymatic activity and application of the cellobiose epimerase mutant. The amino acid sequence of the cellobiose epimerase mutant with improved enzymatic activity is shown as SEQ ID NO.3 or SEQ ID NO.5. The invention further discloses a preparation method of the cellobiose epimerase mutant with improved enzymatic activity. The mutant constructed by the invention can catalyze high-concentration lactose to generate lactulose, and the content of epinose is remarkably reduced. Compared with parent cellobiose epimerase, the mutant provided by the invention has the advantages that the concentration of a catalytic substrate is increased, the by-product brevolactose is reduced, the purity of lactulose is improved, and the mutant has a good application prospect in industrial production of lactulose.

The application belongs to the technical field of pharmaceutical preparations, and discloses a lactulose oral solution and a preparation method thereof.The lactulose oral solution is composed of lactulose, a stabilizer, a flavoring agent, a pH regulator and purified water, wherein the stabilizer is a complex system of cyclodextrin and threonine, and the lactulose and the stabilizer form a lactulose-stabilizer complex liquid through a specific process.Through the synergistic effect of the complex stabilizer, the application effectively inhibits the hydrolysis of lactulose and significantly improves the stability of the preparation;in combination with the preferred flavoring agent, the taste is optimized, and the inherent sweet and greasy taste and peculiar smell of lactulose are covered up;the application is widely applicable to a wide range of people and can meet the individualized medication needs of the elderly, children and other people, improve the clinical treatment effect and the medication compliance of patients, and has important clinical application value and market prospect.

The invention discloses a high-temperature-resistant cellobiose epimerase mutant, an engineering bacterium and application of the high-temperature-resistant cellobiose epimerase mutant. According to the high-temperature-resistant cellobiose epimerase mutant, the half-life period of a mutant enzyme at the temperature of 80 DEG C is prolonged, the half-life period of the mutant DiCE / S184K / K7G / N238P at the temperature of 80 DEG C is 184.2 min and is 4.3 times that of an original enzyme, and the optimal temperature reaches 85 DEG C and is increased by 5 DEG C compared with that before mutation. According to the mutant enzyme disclosed by the invention, the substrate affinity of the enzyme to lactose serving as a substrate for synthesizing lactulose is improved, the conversion efficiency is improved, the yield of the product lactulose is obviously increased, the yield of a by-product epinose is obviously reduced, the yield of the product is increased by 11.8%, and the yield of the by-product is reduced by 1.4%. The method has the advantages of environmental protection, low toxicity, few byproducts and high product yield, overcomes the problem of three wastes easily generated by a chemical synthesis method, and has important industrial application prospects.

The invention relates to the technical field of dairy products, in particular to formula goat milk powder as well as a preparation method and application thereof. The formula goat milk powder comprises the following raw materials: whole goat milk powder, lactulose and a medicinal and edible mixed extract, the medicinal and edible mixed extract is prepared by extracting a mixture of astragalus membranaceus, codonopsis pilosula, fructus cannabis, cistanche, orange peel and dogwood. The formula goat milk powder disclosed by the invention has the effects of tonifying kidney, tonifying qi and moistening intestines on the basis of improving the immunity, not only considers the improvement of'standard ', but also considers the conditioning of'root', and can effectively relieve the problems of slow transit constipation and low immunity of old people. In addition, the formula goat milk powder disclosed by the invention is stable in performance, simple in preparation process and easy for large-scale production, and has a wide application prospect.

The invention discloses a method for treating high-boron, high-salt and high-COD (Chemical Oxygen Demand) wastewater produced by lactulose production in a pharmaceutical factory, which comprises the following steps of: carrying out microfiltration on the wastewater, then carrying out nanofiltration separation to obtain organic matter concentrated water and a borate solution, and carrying out electrodialysis stripping salt on the borate solution to obtain fresh water containing boric acid and concentrated water containing salt, organic matters and boron; enabling the fresh water to pass through a reverse osmosis membrane to obtain boric acid concentrated water and pure water, enabling the boric acid concentrated water to pass through a freezing crystallization centrifugal system to obtain crystal boric acid and mother liquor, performing nanofiltration on the mother liquor to obtain permeate liquid and concentrated liquid, and recycling the permeate liquid and the concentrated liquid; concentrated water is subjected to electrocatalytic oxidation to produce water, the water enters a boron removal resin adsorption tower, the produced water is subjected to bipolar membrane electrodialysis to produce recycled sodium hydroxide and hydrochloric acid, light salt brine is subjected to electrodialysis membrane desalination, formed concentrated water is returned to a bipolar membrane electrodialysis system, and formed fresh water is returned to the first electrodialysis system; after resin in the adsorption tower is saturated, hydrochloric acid is used for regeneration, sodium hydroxide is used for transformation, and recycling is carried out. And all wastewater is utilized.

Provided is a method for preparing lactulose using immobilized β-galactosidase. The method comprises the following steps: pretreating a macroporous weakly basic styrene-based anion exchange resin; mixing the pretreated resin with a β-galactosidase solution and adding same to a buffer, adsorbing same using a shaker, and leaving same to stand after adsorption; then adding a glutaraldehyde solution for cross-linking, and discarding the supernatant to obtain an immobilized enzyme column; adding a magnesium ion solution to a mixed solution of lactose and fructose, and then converting same by means of the immobilized enzyme column to obtain a lactulose conversion solution; and sequentially performing decolorization, ion exchange, vacuum concentration, and sequential simulated moving bed chromatography separation on the lactulose conversion solution to obtain a lactulose solution.

The invention provides a gene expression cassette for improving CsCE expression quantity and application. The gene expression cassette provided by the invention comprises a regulatory element and a target gene, the regulatory element comprises a coding sequence of a fusion tag, and the target gene comprises a coding sequence of CsCE enzyme. The fusion tag can promote the expression quantity of the CsCE enzyme on the level of regulating and controlling protein folding, and the expression quantity of the CsCE enzyme is improved through the synergistic effect of the fusion tag and the CsCE enzyme, so that the volume enzyme activity of the CsCE enzyme is improved, the CsCE enzyme with higher volume enzyme activity can catalyze lactose to be converted into lactulose in the preparation of lactulose, and the lactulose can be used for preparing lactulose. Further, the preparation yield of the lactulose is improved.

Galactooligosaccharides characterized by a high lactulose content and a process for their production are proposed.

The invention discloses a preparation method of a concentrated lactulose solution, which comprises the following steps: by taking lactose as an initial raw material and phenylboronic acid as a protective agent, carrying out differentiated reaction with sodium hydroxide, and carrying out acidification, extraction, ion exchange and concentration on the product to obtain the concentrated lactulose solution. After phenylboronic acid is added, the reaction is better, and few impurities are generated; the solubility of phenylboronic acid in water is very poor and is only 10g / L (20 DEG C), most phenylboronic acid can be separated out after acid adjustment and cooling, phenylboronic acid can be repeatedly used, the cost is greatly reduced, and the boron content can be reduced to 5 ppm or below after dichloromethane or 2-methyltetrahydrofuran is used for extraction; solvent extraction can be avoided, and the same effect can be achieved by connecting two boron removal resin columns in series; the chemical purity of the obtained concentrated lactulose solution reaches 88.0% or above, the impurity B content is smaller than 6%, the impurity C content is smaller than 5%, post-treatment is simple, the molar yield is 90-95%, and the concentrated lactulose solution is suitable for industrial production.

The application discloses a liquid phase detection method for 2'-fucosyllactose and related impurities, and belongs to the technical field of 2'-fucosyllactose detection. The technical problem to be solved is to provide a detection method for 2'-fucosyllactose and related impurities, which is efficient, has excellent durability of a chromatographic column and excellent resolution. The technical solution points are as follows: a chromatographic column ACQUITY UPLC BEH Amide with a long service life is selected, detection parameters of an electrospray detector are introduced and optimized, a traditional differential detector is replaced, strong separation capacity brought by gradient elution is utilized, a relatively simple and neutral mobile phase is used, and the problems of poor separation effect and poor durability of the chromatographic column for some impurities (lactose and fucosyllactose) are solved.

The invention relates to the technical field of bioinformatics, in particular to a mutation screening method of cellobiose epimerase and related equipment. According to the method provided by the embodiment of the invention, a protein diffusion model is adopted to reconstruct the key catalytic region to obtain a regenerated mutant protein skeleton; performing side chain optimization on the mutant protein skeleton by using a deep learning neural network model to obtain a predicted amino acid sequence of the mutant protein skeleton; obtaining a three-dimensional structure of a cellobiose epimerase mutant-substrate compound according to the predicted amino acid sequence and a molecular structure of a substrate, and screening the three-dimensional structure of the cellobiose epimerase mutant-substrate compound; through the mode, the mutation screening efficiency of the key catalytic region can be improved, the mutant capable of improving the conversion efficiency of lactulose can be rapidly screened, and the screening efficiency of the cellobiose epimerase mutant with the improved conversion efficiency of lactulose is improved.

The invention discloses a cellobiose epimerase mutant with high yield of lactulose and application of the cellobiose epimerase mutant, and belongs to the technical field of enzyme engineering. The invention discloses a cellobiose epimerase (Disp-WT enzyme for short) derived from a microorganism Dictyoglomus sp. As a parent, histidine His at the 245th site is replaced with glutamic acid Glu by using a gene mutation technology, and a mutant H2455E is obtained. The optimal catalysis condition of the mutant enzyme H2455E is not changed, but the isomerase activity of the enzyme at 85 DEG C is improved to 254% of the original isomerase activity, and the generation amount of epinose is greatly reduced. The discovery has an important research value for researching more cellobiose epimerase with relatively low isomerase activity.

Presented herein is a galactooligosaccharide characterized by a high raffinose content and a method for its production.

This invention discloses a recombinant vector for expressing N-acetylglucosamine 2-epimerase, a recombinant bacterial strain, and their applications. The recombinant vector is a plasmid containing the gene sequence of the N-acetylglucosamine 2-epimerase mutant shown in SEQ ID No. 1 and a promoter sequence; the promoter sequence contains at least one of the nucleotide sequences shown in SEQ ID Nos. 2-7. A recombinant Bacillus subtilis strain containing the recombinant vector was further constructed. This recombinant bacterial strain can be used in the preparation of the N-acetylglucosamine 2-epimerase mutant or in the catalytic preparation of lactulose using lactose as a substrate. This invention, through expression vector optimization, promoter replacement, and promoter tandem strategies, successfully achieved the efficient secretory expression of the N-acetylglucosamine 2-epimerase mutant K182R using the Bacillus subtilis expression system, providing reliable technical support for the industrial preparation of enzyme preparations or the enzymatic preparation of lactulose.

The application relates to the technical field of a calcium fortification method of food, and particularly discloses a preparation method and application of calcium fortified food for preventing constipation. The preparation method of the calcium fortified food comprises the following steps: wall material: the wall material is added into deionized water and stirred to obtain a wall material solution; the wall material comprises the following raw materials: modified starch, xanthan gum, sodium hydroxymethyl cellulose, magnesium stearate, modified inulin, sodium alginate and fucoidan; core material: a constipation improvement composition, gum arabic, carrageenan, lactulose and perilla seed oil are mixed, uniformly stirred, ground, and sieved to obtain the core material; the core material and the wall material solution are mixed, homogenized by a colloid mill, and spray dried to obtain the calcium fortified food. In the calcium fortified food for preventing constipation prepared by the application, various components cooperate with each other to improve the mechanical properties of the wall material, so that the wall material is not easy to break, and the storage period of the food is prolonged, and meanwhile, the incidence of constipation caused by calcium supplement products can be obviously reduced.

The application discloses a method for preparing a high-purity lactulose solution by continuously circulating sodium metaaluminate, and belongs to the technical field of oligosaccharide preparation. The preparation method of the application can reduce the reaction temperature, improve the lactulose conversion rate and purity, use food-grade activated carbon to adsorb lactulose clear liquid, remove 96.0% of aluminum ions at most, greatly reduce the cost of aluminum ion removal, and recycle sodium metaaluminate prepared by calcining aluminum hydroxide precipitation, thereby reducing the catalyst consumption. The process optimization method provided by the application is simple and feasible, meets the production demand of resource-saving and environment-friendly, and provides beneficial reference for promoting the industrialized production of lactulose prepared by using sodium metaaluminate / alkaline substance as an alkaline complex catalyst.

This invention discloses a cellobiose epimerase mutant, its encoding gene, a recombinant vector constructed from the encoding gene, recombinant microorganisms obtained by transforming the recombinant vector, and an immobilized enzyme prepared from the cellobiose epimerase mutant. This invention also discloses a method for preparing the immobilized enzyme and its application in lactulose synthesis. This invention, through site-directed mutagenesis of wild-type cellobiose epimerases from different sources, screened and obtained cellobiose epimerase mutants AdCE_S173L / P371E and AdCE_S180A / P371E, which possess high catalytic activity, excellent thermal stability, and excellent selectivity. The immobilized enzymes prepared from these mutants maintain a high conversion rate in the synthesis of lactulose from lactose and exhibit good operational stability and reusability, reducing production costs. They are suitable for the efficient biosynthesis of lactulose and have good industrial application potential.