74 results about "Lactulose" patented technology

Prebiotic formulations can modulate the growth and / or activity of microorganisms in the urogenital area. Such prebiotic formulations can comprise isomaltulose along with at least one other prebiotic, such as 1-kestose or lactulose. These microorganisms which are modulated can be directly related to urogenital health, and their modulation can be used for the prevention, control, or treatment of dysbiosis of the urogenital area.

The invention discloses a cellobiose 2-epimerase mutant as well as a gene, an expression vector, recombinant bacteria, a preparation method and application thereof. The mutant is obtained by performing the following point mutation on an amino acid sequence as shown in SEQ ID No.1: S12A, S24A, G131A, G176A, G224A, K46R, K69R, K72R, K91R, K103R, K201R, K219R, K223R, K241R, K263R, K266R, K273R, K284R, K325R, K335R, K349R and K387R. The invention further discloses a preparation method of the mutant. The invention further discloses a gene segment, a plasmid and a recombinant bacterium for recombinant expression of the mutant, and the gene segment, the plasmid and the recombinant bacterium are used for recombinant expression preparation of the mutant. The mutant can be used for continuously and stably catalyzing and preparing lactulose at high temperature, is relatively good in thermal stability and has a relatively good industrial application prospect.

The present invention provides a neomycin-lactulose cocrystal and a preparation method thereof. The cocrystal is prepared from neomycin and lactulose. The powder X-ray diffraction pattern of the neomycin-lactulose cocrystal has diffraction angles 2θ=12.3°±0.2°, 13.9°±0.2°, 14.9°±0.2°, 16.2°±0.2°, 17.3°±0.2°, 18.1°±0.2°, 18.8° The neomycin-lactulose cocrystal of the present invention has characteristic peaks at 14.5°±0.2°, 19.5°±0.2°, 22.1°±0.2°, 23.2°±0.2°, 24.7°±0.2°, 25.4°±0.2°, 27.4°±0.2°, 28.0°±0.2°, 29.2°±0.2°, 29.8°±0.2°, 31.7°±0.2°, and 33.3°±0.2°. Compared with existing neomycin, the neomycin-lactulose cocrystal of the present invention has more stable physical properties and significantly improved bioavailability under the same conditions.

1. Name of the designed product: packing box (milk fruit sugar oral solution). 2. Use of the designed product: for product packing. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo showing the design points: perspective view 1.

This invention discloses a pet food with coat-enhancing, shine-promoting, and coat-protecting effects, its preparation method, and applications. The food comprises the following components: chicken meal, fresh chicken meat, duck meal, sardine meal, chicken liver, sweet potato meal, corn meal, chicken heart, beef bone meal, duck meat pear, chicken oil, and egg yolk granules; coriander root powder, inulin, seaweed powder, beet pulp, flaxseed, fish oil, cranberry powder, carrot, krill, honeysuckle, turmeric, chrysanthemum, mannan oligosaccharide, lactulose, etc. The raw materials and auxiliary ingredients of this pet food are widely available, palatable, inexpensive, and readily available. The coriander root powder, other auxiliary ingredients, and nutritional supplements / additives work synergistically to improve the shine of the pet's coat, promote growth, and maintain coat health, while also improving the microstructure of the hair cuticle, fundamentally preventing pet coat health problems. The pet food preparation method disclosed in this invention is simple to operate, safe, and environmentally friendly, and is particularly suitable for feed products with high humidity and high heat sensitivity.

The invention provides novel injection type glycerine enema which comprises an injection assembly, and the injection assembly comprises an injection tube, a piston, a push rod, a push block, a nipple, a fixing ring, a sealing ring, a gradually-hardened tube, a cavity, an L-shaped plate, a clamping block, a flexible steel rope, a pull ring, a mounting block and a clamping groove. And a piston is connected to the inner wall of the injection tube in a sliding manner. The gradually-hard tube is inserted into a place where the injection tube is inserted, the push block is pushed to drive the push rod to move, the push rod drives the piston to slide on the injection tube, and liquid medicine is pushed in through the gradually-hard tube, outflow and injection caused by two-time extrusion of common glycerine enema are avoided through one-time injection of 20 ml, two-time mucosa damage is avoided, pollution caused by excrement back-pumping after extrusion is finished is avoided, and the injection efficiency is improved. The pull ring is pulled leftwards, the pull ring drives the clamping block to be separated from the clamping groove through the flexible steel rope, the sealing ring is moved to take down the gradually-hard tube, and the gradually-hard tube is disinfected and cleaned respectively, so that the comfort degree is improved, the rectal mucosa is not prone to being damaged, all liquid medicine can be pushed in in a pushing injection mode, and the liquid medicine is not left and wasted.

The embodiment of the invention discloses a preparation method of a concentrated lactulose solution. The method comprises the following steps: by taking a lactose solution as a raw material and sodium sulfite as a catalyst, carrying out an isomerization reaction in the presence of alkali, and then sequentially carrying out primary crystallization, primary centrifugal separation, deionization treatment, concentration, secondary crystallization, secondary centrifugal separation, filtration and concentration adjustment to prepare the concentrated lactulose solution. According to the method, a raw material recycling mode of'reaction-separation-circulation 'is adopted, lactose which does not participate in an isomerization reaction is recycled by adopting two-time crystallization centrifugal separation operation, and the lactose is put into the isomerization reaction again, so that the recycling of the raw materials is realized. The method effectively avoids the problem of environmental pollution caused by boric acid or borate as a catalyst, simplifies the process, reduces the cost, has high product purity, and is suitable for industrial large-scale production.

The problem addressed by this invention is to provide a method for producing a solid molded body excellent in transportation aptitude and solubility, and use thereof. Said problem is solved by a method for producing a solid molded body, the method comprising: a granulation step for granulating a composition containing a protein-containing food (a) and a carbohydrate (b); and a molding step for molding a granulated product obtained in the granulation step, wherein the amount of the carbohydrate (b) is 1-50 mass% with respect to the total amount of the composition, and the carbohydrate (b) contains one or more selected from the group consisting of maltose, erythritol, trehalose, glucose, lactose, lactulose, and dextrin.

The invention provides a gene expression cassette for improving CsCE expression quantity and application. The gene expression cassette provided by the invention comprises a regulatory element and a target gene, the regulatory element comprises at least one of a coding sequence of dtRNA, a coding sequence of a signal peptide and a coding sequence of a fusion tag, and the target gene comprises a coding sequence of CsCE enzyme. Wherein the dtRNA contributes to keeping the structural stability when the CsCE enzyme is transcribed into mRNA, the signal peptide can promote the secretion of the CsCE enzyme, and the fusion tag can assist the correct folding of the CsCE enzyme, so that the expression of the CsCE enzyme can be synergistically regulated under the three-tube alignment of a DNA transcriptional mRNA layer, a protein secretion layer and a protein folding layer, the expression quantity of the CsCE enzyme can be effectively up-regulated, and the expression quantity of the CsCE enzyme can be effectively up-regulated. Therefore, the volume enzyme activity of the CsCE enzyme is improved, and the CsCE enzyme with higher volume enzyme activity can catalyze lactose to be converted into lactulose in the preparation of lactulose, so that the preparation yield of lactulose is improved.

The invention provides a method for separating lactulose and lactose by using a sequential simulated moving bed chromatography system, and relates to the technical field of saccharide separation and purification. According to the invention, the sequential simulated moving bed chromatography system is used for separating and purifying lactulose, and the analysis area, the refining area, the adsorption area and the isolation area can be operated at the same time under the set temperature condition, so that the separation and purification efficiency of lactulose is remarkably improved, continuous large-scale separation and purification of lactulose can be realized, the separation and purification effect is good, the yield is high, and the product quality is good. According to the method, pure water is used as an eluent, so that analyzed and purified compounds are free of damage and toxicity, rich in source and low in cost.

The invention belongs to the technical field of food processing, and provides horse milk powder for improving intestinal health and a preparation technology thereof. The horse milk powder capable of improving intestinal health is prepared from the following components in parts by weight: 60 to 70 parts of whole horse milk powder, 3.0 to 4.0 parts of galactooligosaccharide, 4.0 to 5.0 parts of lactulose, 3.5 to 4.5 parts of beta-glucan, 1.5 to 2.5 parts of inulin, 5.0 to 6.0 parts of complex probiotics, 2.5 to 3.5 parts of pachymaran, 6.5 to 7.5 parts of astragalus polysaccharide and 5.0 to 6.0 parts of calcium carbonate. According to the horse milk powder capable of improving intestinal health disclosed by the invention, medicinal and edible components are added, and probiotics and various prebiotics are compounded, so that the activities of DAO, D-LAC and MPO in intestinal tissues can be remarkably reduced, the contents of acetic acid, propionic acid and butyric acid are increased, and the barrier function of the intestinal tract is improved.

This invention discloses a cellobiose epimerase mutant, engineered bacteria, and its applications. The mutant is obtained by mutating leucine at position 227 of the cellobiose epimerase to valine. The nucleotide sequence of the cellobiose epimerase is shown in SEQ ID NO.1. This invention involves site-directed mutagenesis of existing cellobiose epimerases, maximizing the catalytic performance of the natural enzyme. The resulting mutant exhibits a high lactulose conversion rate. Under optimized conditions, using crude enzyme solution from recombinant bacteria as a catalyst and lactose as a substrate, the conversion rate from lactose to lactulose reaches 70%–73.3%, while the yield of ipilactose is only 7–10%, lower than the byproduct ratio allowed by the Chinese Pharmacopoeia. This demonstrates the potential for large-scale application and has extremely high application value in the industrial production of lactulose.

The application discloses a cellobiose epimerase mutant with high lactulose yield and application thereof, and belongs to the technical field of enzyme engineering. Dictyoglomus The cellobiose epimerase (referred to as Disp-WT enzyme) derived from microorganism Bifidobacterium longum sp. is used as a parent, a gene mutation technology is used, a histidine His at the 245th position is replaced by glutamic acid Glu, and a mutant H245E is obtained.The optimal catalytic condition of the mutant enzyme H245E does not change, but the isomerase activity of the enzyme at 85 DEG C is increased to 254 % of the original, and the generation amount of epi-lactose is greatly reduced.This finding has important research value for researching more cellobiose epimerases with low isomerase activity.

The invention discloses a fermented camel yogurt beverage and a preparation method thereof, and relates to the technical field of fermented dairy products. According to the invention, lactulose is cooperated with a fermentation strain to realize in-vivo and in-vitro dual probiotic synergy, Kluyveromyces marxianus is cooperated with fruit juice to generate natural fragrance, a flavor-guided fermentation technology and a two-stage temperature-controlled fermentation technology are applied, and an ultra-high pressure homogenization technology is combined, so that the flavor-guided fruit juice is obtained. Finally, the fermented camel yogurt beverage which is fine and stable in texture, natural and coordinated in flavor, outstanding in probiotic function, long in shelf life and excellent in weather resistance is obtained.

The invention belongs to the technical field of food, and particularly relates to a preparation method of crystalline lactulose. The preparation method of the crystalline lactulose comprises the following steps: (1) weighing lactulose powder, dissolving in water, heating and stirring to obtain a lactulose solution; (2) adding a composite regulating agent into the lactulose solution obtained in the step (1), and monitoring the viscosity to obtain a mixed solution; (3) adding lactulose crystal seeds into the mixed solution obtained in the step (2) for induced crystallization; then gradient cooling crystallization is adopted; (4) after crystallization is completed, carrying out heat preservation, separation, washing and drying to obtain a lactulose crystal product, and collecting mother liquor for cyclic utilization; according to the preparation method of the crystalline lactulose, the viscosity of the mother liquor can be reduced, the product purity is high, and the yield is high.

The invention belongs to the technical field of chromatographic separation, and particularly relates to a chromatographic column combined lactulose separation method which comprises the following steps: A1, catalytically converting lactose into lactulose to obtain a lactulose conversion solution; a2, adjusting the pH value of the lactulose conversion liquid, performing nanofiltration to obtain a clear liquid and wastewater, performing ion exchange on the clear liquid, and concentrating to obtain a feed liquid; a3, conveying the feed liquid into a first-stage chromatographic separation system, and carrying out first-stage washing separation to obtain a first-stage product containing more than 94 wt% of lactulose and a recovery liquid; and A4, carrying out decoloration, ion exchange and evaporation concentration on the primary product, conveying the primary product into a secondary chromatographic separation system, carrying out secondary washing separation to obtain a secondary product containing more than 99 wt% of lactulose and a waste liquid, and carrying out fixed bed ion exchange, decoloration and evaporation concentration on the secondary product to obtain a lactulose product. According to the method, 99 wt.% of lactulose products are obtained, and a green and environment-friendly process route is opened up for industrial preparation of high-purity lactulose.

The immobilized cell for synthesizing lactulose in the embodiment of the invention comprises a carrier and a host cell immobilized on the carrier, the carrier comprises diatomite, polyethyleneimine and trihydroxymethyl phosphine, and a cellobiose epimerase mutant is synthesized in the host cell; the mass ratio of the host cells to the diatomite is (0.05-3): 1, the ratio of the mass of the host cells to the volume of the polyethyleneimine is 1g: 2.5 mL-12.5 mL, and the ratio of the mass of the host cells to the volume of the trihydroxymethyl phosphine is 1g: 2.5 mL-12.5 mL; by controlling the proportion of the addition amount of the host cell, the diatomite, the polyethyleneimine and the trihydroxymethyl phosphorus in the immobilized cell, the immobilized cell has high stability and retains the catalytic activity of the cellobiose epimerase mutant at the same time, and the lactulose synthesis efficiency can be improved.

Process for the preparation of a galacto-oligosaccharide preparation, which process comprises the step of contacting a lactulose-containing feed with a beta-galactosidase derived from Papiliotrema terrestris. The resulting galacto-oligosaccharide is acceptable to subjects suffering from GOS-related allergy and subjects having lactose intolerance.

The invention belongs to the technical field of lactulose preparation, and particularly relates to a preparation method of high-purity lactulose, which comprises the following steps: preparing lactulose syrup, carrying out nanofiltration purification and concentration, removing boron element by using boron removal resin, removing residual salt by using amphoteric resin, and finally concentrating to obtain a high-purity lactulose concentrated solution. According to the method, the reaction conditions are optimized, the conversion rate of the lactulose is increased, meanwhile, generation of monosaccharide is reduced by controlling the pH value, and the conventional mode that residual salt in the lactulose is removed by alternately using anion exchange resin, cation exchange resin, anion exchange and cation exchange resin is broken through; according to the method, the amphoteric exchange resin is creatively used for removing salt in lactulose at one time, so that the time of a desalting process is greatly shortened, the activation time of the resin is also shortened, purified water required by activation is reduced, the production cost is saved, and the economic benefit is improved.

Method for importing a disaccharide inside a cell The present invention is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The present invention relates to methods for importing a disaccharide inside a cell wherein the disaccharide is lactose, lactulose, N-acetyllactosamine and / or lacto-N-biose. The present invention als relates to the use of the method for the production of a saccharide.

The invention discloses a cellobiose epimerase mutant with improved enzymatic activity and application of the cellobiose epimerase mutant. The amino acid sequence of the cellobiose epimerase mutant with improved enzymatic activity is shown as SEQ ID NO.3 or SEQ ID NO.5. The invention further discloses a preparation method of the cellobiose epimerase mutant with improved enzymatic activity. The mutant constructed by the invention can catalyze high-concentration lactose to generate lactulose, and the content of epinose is remarkably reduced. Compared with parent cellobiose epimerase, the mutant provided by the invention has the advantages that the concentration of a catalytic substrate is increased, the by-product brevolactose is reduced, the purity of lactulose is improved, and the mutant has a good application prospect in industrial production of lactulose.

The present invention discloses a thermostable cellobiose epimerase mutant and its application. The mutant is obtained by a single mutation at position 242 of the amino acid sequence shown in SEQ ID No. 1. The present invention molecularly modifies the cellobiose epimerase (DiCE) from Dictyoglomus thermophilum to screen for mutants with improved thermostability. The resulting mutants have significantly improved lactulose conversion efficiency. Mutant A242L, in particular, can tolerate temperatures of 80°C and has a lactulose conversion rate of 64.41%, showing promising prospects for industrial application.

The present invention discloses a cellobiose epimerase mutant and its application, belonging to the field of bioengineering technology. The mutant is a mutant in which seven amino acids of the amino acid sequence shown in SEQ ID NO.2 are simultaneously subjected to site-directed mutation; the site-directed mutations are: arginine at position 17 is mutated to glutamine, alanine at position 24 is mutated to serine, isoleucine at position 64 is mutated to valine, leucine at position 184 is mutated to serine, phenylalanine at position 243 is mutated to leucine, glutamic acid at position 339 is mutated to aspartic acid, and lysine at position 340 is mutated to isoleucine. The mutant can be applied to the industrial production of lactulose, can efficiently prepare lactulose, and has high production intensity, good catalytic effect on high-concentration substrates, and the enzyme exhibits better stability, can achieve efficient conversion of lactulose, and further provides a favorable foundation for the industrial application of cellobiose epimerase.

The application belongs to the technical field of pharmaceutical preparations, and discloses a lactulose oral solution and a preparation method thereof.The lactulose oral solution is composed of lactulose, a stabilizer, a flavoring agent, a pH regulator and purified water, wherein the stabilizer is a complex system of cyclodextrin and threonine, and the lactulose and the stabilizer form a lactulose-stabilizer complex liquid through a specific process.Through the synergistic effect of the complex stabilizer, the application effectively inhibits the hydrolysis of lactulose and significantly improves the stability of the preparation;in combination with the preferred flavoring agent, the taste is optimized, and the inherent sweet and greasy taste and peculiar smell of lactulose are covered up;the application is widely applicable to a wide range of people and can meet the individualized medication needs of the elderly, children and other people, improve the clinical treatment effect and the medication compliance of patients, and has important clinical application value and market prospect.

The invention relates to the field of enzyme engineering, and provides cellobiose epimerase, a nucleotide sequence, a preparation method and application, the cellobiose epimerase comprises one of H1, H2 and H3, the amino acid sequence of the H1 is shown as SEQ ID NO: 1, the amino acid sequence of the H2 is shown as SEQ ID NO: 2, and the amino acid sequence of the H3 is shown as SEQ ID NO: 3. The cellobiose epimerase has relatively high mass enzyme activity, and is beneficial to improving the conversion rate and the yield of lactulose.

This invention relates to the field of enzyme engineering, and provides a cellobiose epimerase, its nucleotide sequence, preparation method, and application. The cellobiose epimerase includes one of H1, H2, and H3. The amino acid sequence of H1 is shown in SEQ ID NO:1, the amino acid sequence of H2 is shown in SEQ ID NO:2, and the amino acid sequence of H3 is shown in SEQ ID NO:3. This cellobiose epimerase has high quality enzyme activity, which helps to improve the conversion rate and yield of lactulose.

The invention discloses a high-temperature-resistant cellobiose epimerase mutant, an engineering bacterium and application of the high-temperature-resistant cellobiose epimerase mutant. According to the high-temperature-resistant cellobiose epimerase mutant, the half-life period of a mutant enzyme at the temperature of 80 DEG C is prolonged, the half-life period of the mutant DiCE / S184K / K7G / N238P at the temperature of 80 DEG C is 184.2 min and is 4.3 times that of an original enzyme, and the optimal temperature reaches 85 DEG C and is increased by 5 DEG C compared with that before mutation. According to the mutant enzyme disclosed by the invention, the substrate affinity of the enzyme to lactose serving as a substrate for synthesizing lactulose is improved, the conversion efficiency is improved, the yield of the product lactulose is obviously increased, the yield of a by-product epinose is obviously reduced, the yield of the product is increased by 11.8%, and the yield of the by-product is reduced by 1.4%. The method has the advantages of environmental protection, low toxicity, few byproducts and high product yield, overcomes the problem of three wastes easily generated by a chemical synthesis method, and has important industrial application prospects.

The invention relates to the technical field of dairy products, in particular to formula goat milk powder as well as a preparation method and application thereof. The formula goat milk powder comprises the following raw materials: whole goat milk powder, lactulose and a medicinal and edible mixed extract, the medicinal and edible mixed extract is prepared by extracting a mixture of astragalus membranaceus, codonopsis pilosula, fructus cannabis, cistanche, orange peel and dogwood. The formula goat milk powder disclosed by the invention has the effects of tonifying kidney, tonifying qi and moistening intestines on the basis of improving the immunity, not only considers the improvement of'standard ', but also considers the conditioning of'root', and can effectively relieve the problems of slow transit constipation and low immunity of old people. In addition, the formula goat milk powder disclosed by the invention is stable in performance, simple in preparation process and easy for large-scale production, and has a wide application prospect.

The present invention relates to the use of monoester glycolipids or mixtures of monoester glycolipids in laundry detergent compositions. The monoester glycolipids or mixtures of monoester glycolipids include a carbohydrate fraction selected from the group consisting of maltose, cellobiose, trehalose, isomaltulose, lactulose, isomaltose and mixtures thereof.

The invention discloses a method for treating high-boron, high-salt and high-COD (Chemical Oxygen Demand) wastewater produced by lactulose production in a pharmaceutical factory, which comprises the following steps of: carrying out microfiltration on the wastewater, then carrying out nanofiltration separation to obtain organic matter concentrated water and a borate solution, and carrying out electrodialysis stripping salt on the borate solution to obtain fresh water containing boric acid and concentrated water containing salt, organic matters and boron; enabling the fresh water to pass through a reverse osmosis membrane to obtain boric acid concentrated water and pure water, enabling the boric acid concentrated water to pass through a freezing crystallization centrifugal system to obtain crystal boric acid and mother liquor, performing nanofiltration on the mother liquor to obtain permeate liquid and concentrated liquid, and recycling the permeate liquid and the concentrated liquid; concentrated water is subjected to electrocatalytic oxidation to produce water, the water enters a boron removal resin adsorption tower, the produced water is subjected to bipolar membrane electrodialysis to produce recycled sodium hydroxide and hydrochloric acid, light salt brine is subjected to electrodialysis membrane desalination, formed concentrated water is returned to a bipolar membrane electrodialysis system, and formed fresh water is returned to the first electrodialysis system; after resin in the adsorption tower is saturated, hydrochloric acid is used for regeneration, sodium hydroxide is used for transformation, and recycling is carried out. And all wastewater is utilized.