91 results about "Lactulose" patented technology

The invention provides a cellobiose epimerase mutant E250Q, an engineering bacterium and application of the cellobiose epimerase mutant E250Q, and belongs to the technical field of genetic engineering. According to the invention, glutamic acid at the 250th site of an amino acid sequence of wild cellobiose epimerase is mutated into glutamine, so that the cellobiose epimerase mutant E250Q is obtained. The amino acid sequence of the cellobiose epimerase mutant disclosed by the invention is shown as any one of SEQ ID NO.1-6. The invention further discloses a preparation method of the cellobiose epimerase mutant. According to the method, recombinant bacteria or cellobiose epimerase mutant E250Q is used as a catalyst, lactose is used as a substrate, the conversion rate of converting lactose to lactulose reaches 67.1%, the yield of brepinose is only 4.0-5.7% and is lower than the by-product proportion permitted by Chinese Pharmacopoeia, and the method has extremely high application value in industrial production of lactulose.

The invention is applied to agricultural production, and particularly relates to a biostimulant formula and application of the biostimulant formula in promoting or regulating crop growth and inducing disease resistance in agricultural production. The biostimulant active component contains lactulose and nicotinamide mononucleotide; wherein the weight ratio of lactulose to nicotinamide mononucleotide is (1-10): (1-10). The formula of the biostimulant has the functions of promoting crop growth, improving crop quality and inducing disease resistance at the same time. The raw materials required by the formula are low in price and easy to obtain, the preparation process is simple, the equipment requirement is low, and the formula can be applied to the field of agricultural production.

Prebiotic formulations can modulate the growth and / or activity of microorganisms in the urogenital area. Such prebiotic formulations can comprise isomaltulose along with at least one other prebiotic, such as 1-kestose or lactulose. These microorganisms which are modulated can be directly related to urogenital health, and their modulation can be used for the prevention, control, or treatment of dysbiosis of the urogenital area.

The invention discloses a cellobiose 2-epimerase mutant as well as a gene, an expression vector, recombinant bacteria, a preparation method and application thereof. The mutant is obtained by performing the following point mutation on an amino acid sequence as shown in SEQ ID No.1: S12A, S24A, G131A, G176A, G224A, K46R, K69R, K72R, K91R, K103R, K201R, K219R, K223R, K241R, K263R, K266R, K273R, K284R, K325R, K335R, K349R and K387R. The invention further discloses a preparation method of the mutant. The invention further discloses a gene segment, a plasmid and a recombinant bacterium for recombinant expression of the mutant, and the gene segment, the plasmid and the recombinant bacterium are used for recombinant expression preparation of the mutant. The mutant can be used for continuously and stably catalyzing and preparing lactulose at high temperature, is relatively good in thermal stability and has a relatively good industrial application prospect.

The invention discloses an N-acetyl glucosamine 2-epimerase mutant as well as preparation and application of the N-acetyl glucosamine 2-epimerase mutant. The mutant enzyme comprises an amino acid sequence as shown in SEQ ID No.3. The preparation method comprises the following steps: by taking a recombinant vector containing a Sumo enhancing element and a wild type N-acetyl glucosamine 2-epimerase gene as a template, carrying out PCR (Polymerase Chain Reaction) by adopting a mutation primer to obtain a mutant gene segment; transforming the mutant gene segment into an engineering bacterium to obtain a recombinant bacterium for expressing the N-acetyl glucosamine 2-epimerase mutant protein; culturing recombinant bacteria, collecting and crushing thalli, centrifuging thallus crushing liquid, and taking supernatant, so as to obtain crude enzyme liquid containing the enzyme mutant; the thermal stability of the wild-type enzyme is improved through point mutation of specific amino acid sites, and the substrate affinity and the catalytic efficiency of the mutant are superior to those of the wild-type enzyme. The mutant can be used for efficient catalytic preparation of lactulose, and has the advantages of few byproducts, high product yield and the like.

The present invention provides a neomycin-lactulose cocrystal and a preparation method thereof. The cocrystal is prepared from neomycin and lactulose. The powder X-ray diffraction pattern of the neomycin-lactulose cocrystal has diffraction angles 2θ=12.3°±0.2°, 13.9°±0.2°, 14.9°±0.2°, 16.2°±0.2°, 17.3°±0.2°, 18.1°±0.2°, 18.8° The neomycin-lactulose cocrystal of the present invention has characteristic peaks at 14.5°±0.2°, 19.5°±0.2°, 22.1°±0.2°, 23.2°±0.2°, 24.7°±0.2°, 25.4°±0.2°, 27.4°±0.2°, 28.0°±0.2°, 29.2°±0.2°, 29.8°±0.2°, 31.7°±0.2°, and 33.3°±0.2°. Compared with existing neomycin, the neomycin-lactulose cocrystal of the present invention has more stable physical properties and significantly improved bioavailability under the same conditions.

1. Name of the designed product: packing box (milk fruit sugar oral solution). 2. Use of the designed product: for product packing. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo showing the design points: perspective view 1.

This invention discloses a pet food with coat-enhancing, shine-promoting, and coat-protecting effects, its preparation method, and applications. The food comprises the following components: chicken meal, fresh chicken meat, duck meal, sardine meal, chicken liver, sweet potato meal, corn meal, chicken heart, beef bone meal, duck meat pear, chicken oil, and egg yolk granules; coriander root powder, inulin, seaweed powder, beet pulp, flaxseed, fish oil, cranberry powder, carrot, krill, honeysuckle, turmeric, chrysanthemum, mannan oligosaccharide, lactulose, etc. The raw materials and auxiliary ingredients of this pet food are widely available, palatable, inexpensive, and readily available. The coriander root powder, other auxiliary ingredients, and nutritional supplements / additives work synergistically to improve the shine of the pet's coat, promote growth, and maintain coat health, while also improving the microstructure of the hair cuticle, fundamentally preventing pet coat health problems. The pet food preparation method disclosed in this invention is simple to operate, safe, and environmentally friendly, and is particularly suitable for feed products with high humidity and high heat sensitivity.

The invention provides novel injection type glycerine enema which comprises an injection assembly, and the injection assembly comprises an injection tube, a piston, a push rod, a push block, a nipple, a fixing ring, a sealing ring, a gradually-hardened tube, a cavity, an L-shaped plate, a clamping block, a flexible steel rope, a pull ring, a mounting block and a clamping groove. And a piston is connected to the inner wall of the injection tube in a sliding manner. The gradually-hard tube is inserted into a place where the injection tube is inserted, the push block is pushed to drive the push rod to move, the push rod drives the piston to slide on the injection tube, and liquid medicine is pushed in through the gradually-hard tube, outflow and injection caused by two-time extrusion of common glycerine enema are avoided through one-time injection of 20 ml, two-time mucosa damage is avoided, pollution caused by excrement back-pumping after extrusion is finished is avoided, and the injection efficiency is improved. The pull ring is pulled leftwards, the pull ring drives the clamping block to be separated from the clamping groove through the flexible steel rope, the sealing ring is moved to take down the gradually-hard tube, and the gradually-hard tube is disinfected and cleaned respectively, so that the comfort degree is improved, the rectal mucosa is not prone to being damaged, all liquid medicine can be pushed in in a pushing injection mode, and the liquid medicine is not left and wasted.

The present invention relates to the field of pharmaceutical preparations, and in particular, to a sevelamer carbonate dry suspension and a preparation method thereof. In the sevelamer carbonate dry suspension, the dosage of the suspending agent is 0.13 - 0.33% of the total mass, the dosage of the flocculant is 0.05 - 0.39% of the total mass, the dosage of lactulose is 72.53 - 86.90% of the total mass, the dosage of the flavoring agent is 0.34 - 0.72% of the total mass, and the dosage of the sevelamer carbonate crude drug is 12.51 - 26.20% of the total mass. This sevelamer carbonate dry suspension not only has excellent dispersibility, but also can improve its gastrointestinal side effects.

The embodiment of the invention discloses a preparation method of a concentrated lactulose solution. The method comprises the following steps: by taking a lactose solution as a raw material and sodium sulfite as a catalyst, carrying out an isomerization reaction in the presence of alkali, and then sequentially carrying out primary crystallization, primary centrifugal separation, deionization treatment, concentration, secondary crystallization, secondary centrifugal separation, filtration and concentration adjustment to prepare the concentrated lactulose solution. According to the method, a raw material recycling mode of'reaction-separation-circulation 'is adopted, lactose which does not participate in an isomerization reaction is recycled by adopting two-time crystallization centrifugal separation operation, and the lactose is put into the isomerization reaction again, so that the recycling of the raw materials is realized. The method effectively avoids the problem of environmental pollution caused by boric acid or borate as a catalyst, simplifies the process, reduces the cost, has high product purity, and is suitable for industrial large-scale production.

The problem addressed by this invention is to provide a method for producing a solid molded body excellent in transportation aptitude and solubility, and use thereof. Said problem is solved by a method for producing a solid molded body, the method comprising: a granulation step for granulating a composition containing a protein-containing food (a) and a carbohydrate (b); and a molding step for molding a granulated product obtained in the granulation step, wherein the amount of the carbohydrate (b) is 1-50 mass% with respect to the total amount of the composition, and the carbohydrate (b) contains one or more selected from the group consisting of maltose, erythritol, trehalose, glucose, lactose, lactulose, and dextrin.

The invention provides a gene expression cassette for improving CsCE expression quantity and application. The gene expression cassette provided by the invention comprises a regulatory element and a target gene, the regulatory element comprises at least one of a coding sequence of dtRNA, a coding sequence of a signal peptide and a coding sequence of a fusion tag, and the target gene comprises a coding sequence of CsCE enzyme. Wherein the dtRNA contributes to keeping the structural stability when the CsCE enzyme is transcribed into mRNA, the signal peptide can promote the secretion of the CsCE enzyme, and the fusion tag can assist the correct folding of the CsCE enzyme, so that the expression of the CsCE enzyme can be synergistically regulated under the three-tube alignment of a DNA transcriptional mRNA layer, a protein secretion layer and a protein folding layer, the expression quantity of the CsCE enzyme can be effectively up-regulated, and the expression quantity of the CsCE enzyme can be effectively up-regulated. Therefore, the volume enzyme activity of the CsCE enzyme is improved, and the CsCE enzyme with higher volume enzyme activity can catalyze lactose to be converted into lactulose in the preparation of lactulose, so that the preparation yield of lactulose is improved.

The prebiotic formulation is capable of modulating the growth and / or activity of microorganisms in a region of the urogenital system. Such prebiotic formulations can comprise isomaltulose and at least one other prebiotic, such as 1-kestose or lactulose. These modulated microorganisms can be directly related to the health of the urogenital system, and their modulation can be used to prevent, control or treat dysbacteriosis in the region of the urogenital system.

The invention provides an intelligent preparation process and probiotics suitable for intestinal flora. The probiotics provided by the invention are prepared by adopting an intelligent production process, and have the advantages of high production efficiency and uniform proportioning of the probiotics, and the satisfaction degree and credibility of a user on products and services are enhanced. The probiotics at least comprise prebiotics, probiotic strains, a sweetening agent, a filling agent and / or a stabilizing agent; the prebiotics comprise at least one of isomaltooligosacharide, fructo-oligosaccharide, resistant dextrin and inulin; the probiotic strains comprise at least one of lactic acid bacteria, bifidobacterium lactis, lactobacillus casei and lactobacillus rhamnosus; the sweetening agent comprises at least one of sweet sorbitol and erythritol; the filling agent and / or the stabilizing agent comprise at least one of lactitol, lactulose and resistant dextrin. According to the intelligent preparation method, high-quality probiotics suitable for intestinal flora can be prepared in an intelligent preparation mode, and the intelligent preparation method has great benefits on intestinal peristalsis and digestive system of a human body.

The invention provides a method for separating lactulose and lactose by using a sequential simulated moving bed chromatography system, and relates to the technical field of saccharide separation and purification. According to the invention, the sequential simulated moving bed chromatography system is used for separating and purifying lactulose, and the analysis area, the refining area, the adsorption area and the isolation area can be operated at the same time under the set temperature condition, so that the separation and purification efficiency of lactulose is remarkably improved, continuous large-scale separation and purification of lactulose can be realized, the separation and purification effect is good, the yield is high, and the product quality is good. According to the method, pure water is used as an eluent, so that analyzed and purified compounds are free of damage and toxicity, rich in source and low in cost.

The invention belongs to the technical field of food processing, and provides horse milk powder for improving intestinal health and a preparation technology thereof. The horse milk powder capable of improving intestinal health is prepared from the following components in parts by weight: 60 to 70 parts of whole horse milk powder, 3.0 to 4.0 parts of galactooligosaccharide, 4.0 to 5.0 parts of lactulose, 3.5 to 4.5 parts of beta-glucan, 1.5 to 2.5 parts of inulin, 5.0 to 6.0 parts of complex probiotics, 2.5 to 3.5 parts of pachymaran, 6.5 to 7.5 parts of astragalus polysaccharide and 5.0 to 6.0 parts of calcium carbonate. According to the horse milk powder capable of improving intestinal health disclosed by the invention, medicinal and edible components are added, and probiotics and various prebiotics are compounded, so that the activities of DAO, D-LAC and MPO in intestinal tissues can be remarkably reduced, the contents of acetic acid, propionic acid and butyric acid are increased, and the barrier function of the intestinal tract is improved.

This invention discloses a cellobiose epimerase mutant, engineered bacteria, and its applications. The mutant is obtained by mutating leucine at position 227 of the cellobiose epimerase to valine. The nucleotide sequence of the cellobiose epimerase is shown in SEQ ID NO.1. This invention involves site-directed mutagenesis of existing cellobiose epimerases, maximizing the catalytic performance of the natural enzyme. The resulting mutant exhibits a high lactulose conversion rate. Under optimized conditions, using crude enzyme solution from recombinant bacteria as a catalyst and lactose as a substrate, the conversion rate from lactose to lactulose reaches 70%–73.3%, while the yield of ipilactose is only 7–10%, lower than the byproduct ratio allowed by the Chinese Pharmacopoeia. This demonstrates the potential for large-scale application and has extremely high application value in the industrial production of lactulose.

The application discloses a cellobiose epimerase mutant with high lactulose yield and application thereof, and belongs to the technical field of enzyme engineering. Dictyoglomus The cellobiose epimerase (referred to as Disp-WT enzyme) derived from microorganism Bifidobacterium longum sp. is used as a parent, a gene mutation technology is used, a histidine His at the 245th position is replaced by glutamic acid Glu, and a mutant H245E is obtained.The optimal catalytic condition of the mutant enzyme H245E does not change, but the isomerase activity of the enzyme at 85 DEG C is increased to 254 % of the original, and the generation amount of epi-lactose is greatly reduced.This finding has important research value for researching more cellobiose epimerases with low isomerase activity.

The invention discloses a fermented camel yogurt beverage and a preparation method thereof, and relates to the technical field of fermented dairy products. According to the invention, lactulose is cooperated with a fermentation strain to realize in-vivo and in-vitro dual probiotic synergy, Kluyveromyces marxianus is cooperated with fruit juice to generate natural fragrance, a flavor-guided fermentation technology and a two-stage temperature-controlled fermentation technology are applied, and an ultra-high pressure homogenization technology is combined, so that the flavor-guided fruit juice is obtained. Finally, the fermented camel yogurt beverage which is fine and stable in texture, natural and coordinated in flavor, outstanding in probiotic function, long in shelf life and excellent in weather resistance is obtained.

The invention belongs to the technical field of food, and particularly relates to a preparation method of crystalline lactulose. The preparation method of the crystalline lactulose comprises the following steps: (1) weighing lactulose powder, dissolving in water, heating and stirring to obtain a lactulose solution; (2) adding a composite regulating agent into the lactulose solution obtained in the step (1), and monitoring the viscosity to obtain a mixed solution; (3) adding lactulose crystal seeds into the mixed solution obtained in the step (2) for induced crystallization; then gradient cooling crystallization is adopted; (4) after crystallization is completed, carrying out heat preservation, separation, washing and drying to obtain a lactulose crystal product, and collecting mother liquor for cyclic utilization; according to the preparation method of the crystalline lactulose, the viscosity of the mother liquor can be reduced, the product purity is high, and the yield is high.

The invention belongs to the technical field of chromatographic separation, and particularly relates to a chromatographic column combined lactulose separation method which comprises the following steps: A1, catalytically converting lactose into lactulose to obtain a lactulose conversion solution; a2, adjusting the pH value of the lactulose conversion liquid, performing nanofiltration to obtain a clear liquid and wastewater, performing ion exchange on the clear liquid, and concentrating to obtain a feed liquid; a3, conveying the feed liquid into a first-stage chromatographic separation system, and carrying out first-stage washing separation to obtain a first-stage product containing more than 94 wt% of lactulose and a recovery liquid; and A4, carrying out decoloration, ion exchange and evaporation concentration on the primary product, conveying the primary product into a secondary chromatographic separation system, carrying out secondary washing separation to obtain a secondary product containing more than 99 wt% of lactulose and a waste liquid, and carrying out fixed bed ion exchange, decoloration and evaporation concentration on the secondary product to obtain a lactulose product. According to the method, 99 wt.% of lactulose products are obtained, and a green and environment-friendly process route is opened up for industrial preparation of high-purity lactulose.

The immobilized cell for synthesizing lactulose in the embodiment of the invention comprises a carrier and a host cell immobilized on the carrier, the carrier comprises diatomite, polyethyleneimine and trihydroxymethyl phosphine, and a cellobiose epimerase mutant is synthesized in the host cell; the mass ratio of the host cells to the diatomite is (0.05-3): 1, the ratio of the mass of the host cells to the volume of the polyethyleneimine is 1g: 2.5 mL-12.5 mL, and the ratio of the mass of the host cells to the volume of the trihydroxymethyl phosphine is 1g: 2.5 mL-12.5 mL; by controlling the proportion of the addition amount of the host cell, the diatomite, the polyethyleneimine and the trihydroxymethyl phosphorus in the immobilized cell, the immobilized cell has high stability and retains the catalytic activity of the cellobiose epimerase mutant at the same time, and the lactulose synthesis efficiency can be improved.

Process for the preparation of a galacto-oligosaccharide preparation, which process comprises the step of contacting a lactulose-containing feed with a beta-galactosidase derived from Papiliotrema terrestris. The resulting galacto-oligosaccharide is acceptable to subjects suffering from GOS-related allergy and subjects having lactose intolerance.

The invention belongs to the technical field of lactulose preparation, and particularly relates to a preparation method of high-purity lactulose, which comprises the following steps: preparing lactulose syrup, carrying out nanofiltration purification and concentration, removing boron element by using boron removal resin, removing residual salt by using amphoteric resin, and finally concentrating to obtain a high-purity lactulose concentrated solution. According to the method, the reaction conditions are optimized, the conversion rate of the lactulose is increased, meanwhile, generation of monosaccharide is reduced by controlling the pH value, and the conventional mode that residual salt in the lactulose is removed by alternately using anion exchange resin, cation exchange resin, anion exchange and cation exchange resin is broken through; according to the method, the amphoteric exchange resin is creatively used for removing salt in lactulose at one time, so that the time of a desalting process is greatly shortened, the activation time of the resin is also shortened, purified water required by activation is reduced, the production cost is saved, and the economic benefit is improved.

Method for importing a disaccharide inside a cell The present invention is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The present invention relates to methods for importing a disaccharide inside a cell wherein the disaccharide is lactose, lactulose, N-acetyllactosamine and / or lacto-N-biose. The present invention als relates to the use of the method for the production of a saccharide.

The invention discloses a rhamnosus lactobacillus that does not utilize lactulose but can utilize galactose and its application. The strain is rhamnosus lactobacillus grx21, and its preservation number is CGMCC No.26009. The rhamnosus lactobacillus grx21 of the invention can utilize galactose but cannot utilize lactulose, has a fast metabolic rate and a high survival rate, can be used to remove the byproduct galactose in the hot alkali synthesis lactulose system, is used to prepare high-purity lactulose suitable for use by people with galactosemia, and is used as a probiotic in food or medicine.

The invention discloses a cellobiose epimerase mutant with improved enzymatic activity and application of the cellobiose epimerase mutant. The amino acid sequence of the cellobiose epimerase mutant with improved enzymatic activity is shown as SEQ ID NO.3 or SEQ ID NO.5. The invention further discloses a preparation method of the cellobiose epimerase mutant with improved enzymatic activity. The mutant constructed by the invention can catalyze high-concentration lactose to generate lactulose, and the content of epinose is remarkably reduced. Compared with parent cellobiose epimerase, the mutant provided by the invention has the advantages that the concentration of a catalytic substrate is increased, the by-product brevolactose is reduced, the purity of lactulose is improved, and the mutant has a good application prospect in industrial production of lactulose.

The present invention discloses a thermostable cellobiose epimerase mutant and its application. The mutant is obtained by a single mutation at position 242 of the amino acid sequence shown in SEQ ID No. 1. The present invention molecularly modifies the cellobiose epimerase (DiCE) from Dictyoglomus thermophilum to screen for mutants with improved thermostability. The resulting mutants have significantly improved lactulose conversion efficiency. Mutant A242L, in particular, can tolerate temperatures of 80°C and has a lactulose conversion rate of 64.41%, showing promising prospects for industrial application.

The present invention discloses a cellobiose epimerase mutant and its application, belonging to the field of bioengineering technology. The mutant is a mutant in which seven amino acids of the amino acid sequence shown in SEQ ID NO.2 are simultaneously subjected to site-directed mutation; the site-directed mutations are: arginine at position 17 is mutated to glutamine, alanine at position 24 is mutated to serine, isoleucine at position 64 is mutated to valine, leucine at position 184 is mutated to serine, phenylalanine at position 243 is mutated to leucine, glutamic acid at position 339 is mutated to aspartic acid, and lysine at position 340 is mutated to isoleucine. The mutant can be applied to the industrial production of lactulose, can efficiently prepare lactulose, and has high production intensity, good catalytic effect on high-concentration substrates, and the enzyme exhibits better stability, can achieve efficient conversion of lactulose, and further provides a favorable foundation for the industrial application of cellobiose epimerase.