10 results about "Lactulose" patented technology

This invention discloses a pet food with coat-enhancing, shine-promoting, and coat-protecting effects, its preparation method, and applications. The food comprises the following components: chicken meal, fresh chicken meat, duck meal, sardine meal, chicken liver, sweet potato meal, corn meal, chicken heart, beef bone meal, duck meat pear, chicken oil, and egg yolk granules; coriander root powder, inulin, seaweed powder, beet pulp, flaxseed, fish oil, cranberry powder, carrot, krill, honeysuckle, turmeric, chrysanthemum, mannan oligosaccharide, lactulose, etc. The raw materials and auxiliary ingredients of this pet food are widely available, palatable, inexpensive, and readily available. The coriander root powder, other auxiliary ingredients, and nutritional supplements / additives work synergistically to improve the shine of the pet's coat, promote growth, and maintain coat health, while also improving the microstructure of the hair cuticle, fundamentally preventing pet coat health problems. The pet food preparation method disclosed in this invention is simple to operate, safe, and environmentally friendly, and is particularly suitable for feed products with high humidity and high heat sensitivity.

The application belongs to the technical field of pharmaceutical preparations, and discloses a lactulose oral solution and a preparation method thereof.The lactulose oral solution is composed of lactulose, a stabilizer, a flavoring agent, a pH regulator and purified water, wherein the stabilizer is a complex system of cyclodextrin and threonine, and the lactulose and the stabilizer form a lactulose-stabilizer complex liquid through a specific process.Through the synergistic effect of the complex stabilizer, the application effectively inhibits the hydrolysis of lactulose and significantly improves the stability of the preparation;in combination with the preferred flavoring agent, the taste is optimized, and the inherent sweet and greasy taste and peculiar smell of lactulose are covered up;the application is widely applicable to a wide range of people and can meet the individualized medication needs of the elderly, children and other people, improve the clinical treatment effect and the medication compliance of patients, and has important clinical application value and market prospect.

Provided is a method for preparing lactulose using immobilized β-galactosidase. The method comprises the following steps: pretreating a macroporous weakly basic styrene-based anion exchange resin; mixing the pretreated resin with a β-galactosidase solution and adding same to a buffer, adsorbing same using a shaker, and leaving same to stand after adsorption; then adding a glutaraldehyde solution for cross-linking, and discarding the supernatant to obtain an immobilized enzyme column; adding a magnesium ion solution to a mixed solution of lactose and fructose, and then converting same by means of the immobilized enzyme column to obtain a lactulose conversion solution; and sequentially performing decolorization, ion exchange, vacuum concentration, and sequential simulated moving bed chromatography separation on the lactulose conversion solution to obtain a lactulose solution.

Galactooligosaccharides characterized by a high lactulose content and a process for their production are proposed.

Presented herein is a galactooligosaccharide characterized by a high raffinose content and a method for its production.

This invention discloses a recombinant vector for expressing N-acetylglucosamine 2-epimerase, a recombinant bacterial strain, and their applications. The recombinant vector is a plasmid containing the gene sequence of the N-acetylglucosamine 2-epimerase mutant shown in SEQ ID No. 1 and a promoter sequence; the promoter sequence contains at least one of the nucleotide sequences shown in SEQ ID Nos. 2-7. A recombinant Bacillus subtilis strain containing the recombinant vector was further constructed. This recombinant bacterial strain can be used in the preparation of the N-acetylglucosamine 2-epimerase mutant or in the catalytic preparation of lactulose using lactose as a substrate. This invention, through expression vector optimization, promoter replacement, and promoter tandem strategies, successfully achieved the efficient secretory expression of the N-acetylglucosamine 2-epimerase mutant K182R using the Bacillus subtilis expression system, providing reliable technical support for the industrial preparation of enzyme preparations or the enzymatic preparation of lactulose.

This invention discloses a cellobiose epimerase mutant, its encoding gene, a recombinant vector constructed from the encoding gene, recombinant microorganisms obtained by transforming the recombinant vector, and an immobilized enzyme prepared from the cellobiose epimerase mutant. This invention also discloses a method for preparing the immobilized enzyme and its application in lactulose synthesis. This invention, through site-directed mutagenesis of wild-type cellobiose epimerases from different sources, screened and obtained cellobiose epimerase mutants AdCE_S173L / P371E and AdCE_S180A / P371E, which possess high catalytic activity, excellent thermal stability, and excellent selectivity. The immobilized enzymes prepared from these mutants maintain a high conversion rate in the synthesis of lactulose from lactose and exhibit good operational stability and reusability, reducing production costs. They are suitable for the efficient biosynthesis of lactulose and have good industrial application potential.

The application discloses a cellobiose epimerase mutant with improved thermal stability and application thereof in synthesis of lactulose. Caldicellulosiruptor saccharolyticus The enzyme catalysis system for high-efficiency conversion of lactose to lactulose is constructed by performing thermal stability modification on the starting enzyme CsCE from The application has the advantages of green environmental protection, low toxicity, less by-products and high product yield, overcomes the three wastes problems easily generated by the chemical synthesis method, and has important industrial application prospect.

The use of 1-lactulose in the preparation of drugs for preventing and treating liver injury or liver fibrosis has the advantage of significant effects in preventing and treating liver injury or liver fibrosis.

This invention discloses a method for dynamically regulating and efficiently producing cellobiose epimerases and its application in lactulose production, belonging to the fields of biotechnology and enzyme engineering. This invention integrates and replaces the promoter mutant P in the Bacillus subtilis genome. ugtP M1 P ugtP M2 P ugtP M3 The QS system and prkC gene were integrated into the amyE site of the genome, and the plasmid pMA5-CS-CE was further transformed into the recombinant strain. Cellobiose epimerase was produced by shake-flask fermentation, with an enzyme activity of 16.05 U / mL; after 24 h of fermentation in a 5 L fermenter, the cellobiose epimerase activity reached 21.52 U / mL. The cellobiose epimerase prepared in this invention can catalyze high concentrations of lactose, yielding lactulose production of up to 375 g·L⁻¹. ‑1 The lactulose conversion rate is 75%.