713 results about "Immunologic function" patented technology

The invention discloses bifidobacterium breve BBr69 with an immunoregulation function as well as application, a product and a method thereof, and belongs to the technical field of microorganisms. The preservation number of the bifidobacterium breve BBr69 with the immunoregulation function is CGMCC (China General Microbiological Culture Collection Center) NO. 32425. Based on the strain, the invention also provides application and related products of the strain. The BBr69 strain has broad-spectrum antibacterial performance, has the function of regulating intestinal flora and also has the function of enhancing immunity, and immunity is enhanced by proliferating RAW264.7 cells, increasing the content of cell factors, inhibiting weight loss, increasing the index of immune organs and reducing ileum tissue damage.

The invention discloses application of a ganoderma lucidum strain SS30 in preparation of a product for treating cancers, and belongs to the technical field of biology. The invention provides ganoderma lucidum strain SS30 mycelium polysaccharide. The ganoderma lucidum strain SS30 mycelium polysaccharide is prepared from glucose, galactose, mannose, fucose, xylose, arabinose, rhamnose, glucuronic acid, glucosamine, ribose and lacturonic acid. The polysaccharide has a remarkable effect of inhibiting transplantable pancreatic cancer and primary colorectal cancer, can achieve the effect of treating cancer by improving the immune function and reducing the oxidation product generation level, provides a new material for development and utilization of lucid ganoderma and products thereof, and has a wide application prospect. And a research basis is provided for research and development of cancer-related drugs and research of a cancer treatment mechanism.

To provide beta-1,3-glucan, and to provide uses thereof for regulating immunity in the human body, which are methods for treating and / or preventing high cholesterol, diabetes, and allergies, and methods for treating and / or preventing intestinal inflammation.SOLUTION: Provided is a composition for oral administration for alleviating symptoms associated with intestinal inflammation in humans, the composition containing an effective amount of beta-1,3-glucan dried to a moisture content of about 40% or less from Euglena grown by fermentation, the beta-1,3-glucan not containing beta-1,6-glycosidic bonds, the effective amount being between 0.1 mg of beta-1,3-glucan / kg body weight and 100 mg of beta-1,3-glucan / kg body weight.SELECTED DRAWING: Figure 1

The invention discloses a metabolite screening method for promoting expression of porcine intestinal defensin pBD1 and application of the metabolite screening method. The screening method comprises the following steps: 1) constructing a porcine defensin pBD1 gene fluorescence report intestinal epithelial cell line; 2) evaluating the promotion effects of different metabolites based on the pBD1 gene fluorescence report intestinal epithelial cell line; and 3) further evaluating the promotion effects of the metabolites screened in the step 2) based on a porcine intestinal epithelial cell IPEC-J2 model. And 4) further evaluating the promotion effect of the metabolite screened in the step 3) based on the pig intestinal organ. The invention can be used for preparing or screening drugs influencing the expression of the porcine intestinal defensin pBD1. According to the application disclosed by the invention, the effect of the D-malic acid for improving the immune function of the pig intestinal tract by promoting the expression of the defensin pBD1 is found for the first time, verification is carried out on a pig intestinal tract organ-like model, and meanwhile, the optimal treatment concentration of the D-malic acid for promoting the expression of the defensin pBD1 in the pig intestinal tract is optimized.

The invention relates to a bifidobacterium longum subsp. Infantis Imu-01 and an application thereof. Specifically, the invention provides bifidobacterium longum subsp.infantis Imu-01, a culture, a preparation and application of the bifidobacterium longum subsp.infantis Imu-01 in preparation of a product for improving the immune function. The bifidobacterium longum subsp. Infantis provided by the invention can regulate and control the immune level of an object, improve the content of butyric acid in intestinal tracts and improve the utilization of oligosaccharide in breast milk.

The invention relates to the technical field of microorganisms, and particularly discloses a fermentation method of bifidobacterium longum BBMN68 as well as a fermented product, a postbiotic and application of the fermented product. According to the method for fermenting the bifidobacterium longum BBMN68, a fermentation substrate comprises alkaline protease hydrolyzed milk and a pomelo peel extract; the hydrolysis rate of the alkaline protease hydrolyzed milk is 9-11%, and the water-soluble dietary fiber content of the shaddock peel extract is 75-80%; the mass ratio of the alkaline protease hydrolyzed milk to the shaddock peel extract is (10-15): (1-3). The content of active ingredients in the fermented product prepared by the method is high, and after the fermented product is prepared into the postbiotics to be taken, the immune function of an organism can be recovered, immune organ damage is reduced, inflammation is relieved, and intestinal flora is regulated and controlled.

The invention discloses lactobacillus reuteri LR69 capable of enhancing a gastric mucosal barrier and application, a product and a method thereof, and belongs to the technical field of microorganisms. The invention provides a lactobacillus reuteri LR69, and the preservation number of the lactobacillus reuteri LR69 is CGMCC (China General Microbiological Culture Collection Center) No.33731. The lactobacillus reuteri LR69 is a lactobacillus reuteri LR69. The invention also provides application of the strain in preparation of food with an auxiliary protection function on gastric mucosa, and / or food with an intestinal flora regulating function, and / or a product for relieving gastritis, and / or food with an immunity enhancing function, and / or a bacteriostatic agent. The invention further discloses a food with an auxiliary protection function on gastric mucosa, a food with an intestinal flora regulating function, a product for relieving gastritis, a food with an immunity enhancing function, a bacteriostatic agent and an in-vitro bacteriostasis method based on the strain. The lactobacillus reuteri LR69 disclosed by the invention can effectively assist in protecting gastric mucosa, regulating intestinal flora, relieving gastritis, enhancing immunity and realizing broad-spectrum bacteriostasis.

The present invention addresses the problem of providing a composition for improving the immunostimulatory effect of lactic acid bacteria, preferably Lactobacillus plantarum L-137. The problem is solved by this composition containing turmeric extract.

The invention relates to the technical field of immune carrier microspheres, in particular to an immune carrier microsphere constructed by individualized MHC-II combined polypeptide, a vaccine and application of the immune carrier microsphere and the vaccine. A core comprises microspheres constructed by a plurality of MHC-II binding polypeptides, sequences of the MHC-II binding polypeptides are obtained based on HLA gene typing results of an inoculator, and each HLA gene corresponds to at least one high-affinity MHC-II binding polypeptide; a shell is a glucan with sulfydryl or other polymer coating layer, and sulfydryl is used as a coupling group to be connected with an antigen to construct the vaccine. The method has the advantages that competition is avoided, inhibition is generated, a better immune effect is achieved, antibodies of high-titer carrier molecules are not generated, and cross reaction side effects are avoided. The carrier molecular diversity is reduced; side effects are avoided. And antibody immunity and cellular immunity functions are generated. The particle size of the microspheres is controllable, and the immunologic function can be achieved without adjuvants. The preparation process is simple to operate and good in repeatability.

The invention relates to a construction method of a macrophage specific fluorescence report mouse model, and belongs to the technical field of genetic engineering and genetic modification. According to the invention, a brand-new C1qc-CreChy gene knock-in mouse model is obtained after a C1qc gene locus is edited through a CRISPR / Cas9 genome editing technology for the first time, the expression of Cre and mCherry is directly driven by a C1qc promoter, and flow cytometry verifies that mCherry has very high expression quantity only in microglial cells in the brain of a mouse and macrophages in the spleen of the mouse. And mCherry expression is not detected in other immune cells, so that the two macrophages are traced and used for researching the functions of the macrophages. The invention provides a usable animal model for researching heterogeneity of macrophages, and provides a new animal model for researching complex immune functions in a body.

The invention discloses a functional probiotic composition and application thereof, and relates to the technical field of microbial engineering. Bifidobacterium longum, lactobacillus acidophilus and enterococcus faecalis are combined to serve as a microbial agent, the microbial agent and active substances of the tea leaves form a composition, then the composition is combined with prebiotics, it is ensured that viable bacteria directly reach the intestinal tract through a microencapsulation technology, and the effect of improving the immune function is achieved; tea polyphenol in the active substances of the tea leaves can serve as a carbon source of prebiotics to stimulate proliferation of the bifidobacterium longum and the lactobacillus acidophilus, so that the acid resistance and intestinal colonization capacity of the microbial inoculum are enhanced, and meanwhile, theanine in the active substances of the tea leaves, the bifidobacterium longum and the lactobacillus acidophilus synergistically promote secretion of gamma-aminobutyric acid, so that the effect of improving the intestinal colonization is achieved. And the tea polyphenol can inhibit the large intestine rod from adhering to the intestinal mucosa, and the probiotic agent secretes antibacterial peptide, so that the integrity of the intestinal barrier is maintained together.

The invention relates to a bactericidal composition for asparagus stem blight, which belongs to the technical field of asparagus stem blight and has good prevention, protection and treatment effects. According to the action mechanism, normal growth of pathogenic bacteria is interfered by inhibiting biosynthesis of ergosterol, a good inhibiting effect on pathogenic bacteria of various plants is achieved, and meanwhile, difenoconazole also has a strong inhibiting effect on spore formation of the pathogenic bacteria of the plants; the chitosan oligosaccharide, the carbendazim, the Chinese herbal medicine active extract, the methanol extract, the plant extract, the difenoconazole, the lentinan and the amino-oligosaccharin lentinan mixed preparation have a good prevention and treatment effect on the asparagus stem blight and can block combination of pathogens and sugar molecules on the surfaces of normal cells through an adhesion effect, so that the infection path of the asparagus stem blight is blocked. The lentinan has potential value in the aspect of preventing and treating the asparagus stem blight, and the lentinan enhances the immune function and blocks the pathogen infection pathway.

The invention relates to a Lactobacillus paracasei LC97, and an application, a product and a method thereof, and belongs to the technical field of microorganisms. The preservation number of the lactobacillus paracasei LC97 for relieving allergy is CGMCC (China General Microbiological Culture Collection Center) NO. 32421. Based on the strain, the invention also provides application and related products of the strain. The LC97 strain has broad-spectrum antibacterial performance, has the functions of enhancing immune function and relieving atopic dermatitis, and can relieve atopic dermatitis by relieving skin lesion, inhibiting inflammation, reducing ear swelling degree and regulating cell factor level. And by reducing the total levels of serum IgE and IL-4, the levels of IFN-gamma and IL-10 are improved, and the levels of cytokines are regulated, so that the immunity is enhanced.

The invention relates to application of 4-GBA in preparation of a medicine for improving infection endurance capacity of a sepsis patient, 4-GBA is used for treating mice infected with sepsis, compared with control mice, the survival rate of the mice treated by the 4-GBA is far larger than that of the control mice, and experiments prove that the 4-GBA has a protection effect on sepsis infection. The mechanism of 4-GBA for improving infection endurance capacity of sepsis patients is researched, and after 4-GBA treatment, the content of liver and kidney functional markers in peripheral blood serum of mice infected with sepsis is remarkably reduced, the content of DAMPs (injury related molecular patterns) related molecules in peripheral blood serum of mice infected with sepsis is remarkably reduced, and the application of 4-GBA in the treatment of the mice infected with sepsis in the treatment of the infection endurance capacity of the patients infected with sepsis in the treatment of the infection endurance capacity of the patients infected with sepsis in the treatment of the patients infected with sepsis in the treatment of the patients infected with sepsis. And the product does not have in-vivo and in-vitro sterilization functions and body immune regulation functions. The result shows that 4-GBA can improve the infection endurance capacity of the sepsis patient, and provides a new direction and a new drug for treatment of the sepsis patient.

The invention provides a fermented feed formula for enhancing animal immune function and a preparation process thereof, and belongs to the field of fermented feed. The preparation process comprises the following steps: preparing the tortoise shell extract; preparing a plant extract; and preparing the fermented feed. The tortoise shell extract and the acacia farnesiana extract are prepared respectively and then added into the fermentation base material to prepare the fermented feed, so that the tortoise shell extract can provide a material basis for proliferation of immune cells and synthesis of immunocompetence substances; the tortoise shell extract creates a good internal environment for normal function exertion of an immune system, in addition, microelements in the tortoise shell extract are matched with bioactive components in the acacia extract, uptake and utilization of the microelements by immune cells are further enhanced, so that the immune regulation effect is more effectively exerted, and the immunity of the human body is improved. The effect of synergistically enhancing the animal immune function is achieved.

To provide an oral composition for effectively activating plasmacytoid dendritic cells (pDCs).SOLUTION: An oral composition for activating plasmacytoid dendritic cells (pDCs), comprising Lacticaseibacillus rhamnosus CRL1505, wherein the composition is preferably intended for immune activation. The oral composition for activating pDCs is considered to: regulate both innate immunity and acquired immunity; act on an overall immune function, maintain a normal immune function, and maintain good physical conditions. The present invention also provides an oral composition for immune activation characterised in that it comprises Lacticaseibacillus rhamnosus CRL1505, wherein the immune activation is intended for symptoms such as nasal congestion.SELECTED DRAWING: Figure 1

The invention relates to application of polypeptide in preparation of a feed additive for improving disease resistance of prawns, and belongs to the technical field of aquaculture. The polypeptide is anglerfish bone polypeptide, and the molecular weight of the anglerfish bone polypeptide is 1000-5000 Da. The anglerfish bone polypeptide prepared by fermentation pretreatment and enzymolysis processes can effectively improve the growth performance, disease resistance and immune function of litopenaeus vannamei. The prawns fed with the feed additive show significant final weight gain and relatively high survival rate, and especially show strong resistance when coping with infection of common pathogenic bacteria such as vibrio parahaemolyticus and the like. Therefore, the survival rate and the production efficiency of the prawns in the culture process are improved, the use of antibiotics can be reduced, the drug residues and the drug resistance of pathogenic bacteria are reduced, and the development of the green and sustainable prawn culture industry is promoted.

The invention relates to the technical field of microorganisms, and discloses a lactobacillus delbrueckii subsp. Bulgaricus XJFY201503 and an application thereof in improving an immune function, the lactobacillus delbrueckii subsp. Bulgaricus XJFY201503 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.30047, and the preservation time is March 18, 2024. The lactobacillus delbrueckii subsp. Bulgaricus XJFY201503 can be used for directly improving the immune function of an immunosuppressive mouse by protecting and maintaining normal physiological structures of central and peripheral immune organs and promoting secretion of serum cytokines and immune globulins; and the immune function of the mouse can be indirectly promoted by relieving intestinal inflammation reaction.

The invention relates to the technical field of microorganisms, in particular to bifidobacterium bifidum BGLP1 capable of regulating sugar digestion and producing GLP-1 at high yield and application of the bifidobacterium bifidum BGLP1. The preservation number of the bifidobacterium bifidum BGLP1 is CGMCC (China General Microbiological Culture Collection Center) No.35718, and the bifidobacterium bifidum BGLP1 can be used for preparing the feed additive. The strain has the capability of inhibiting alpha-glucosidase and alpha-amylase; sTC-1 cells are stimulated to secrete GLP-1, and the blood glucose reducing pathway capacity of the intestinal tract is activated; the activity of pancreatic lipase is obviously inhibited, and the dietary fat absorption capability can be effectively reduced; redundant cane sugar is converted into exopolysaccharide, so that the probiotic function capability is improved; the capability of converting white fat into brown fat is promoted; macrophages are activated, and the body immune function is improved. In addition, the bifidobacterium bifidum BGLP1 also has relatively strong intestinal colonization ability, has good tolerance in artificial gastric juice and artificial intestinal juice, and can smoothly reach the intestinal tract of a human body.

The invention belongs to the technical field of active peptide preparation, and particularly relates to an active collagen peptide with an immunoregulation function and a preparation method and application thereof. The active collagen peptide with an immunoregulation function is prepared by using a method of performing composite enzymolysis and separating and collecting specified collection liquid by using a chromatographic column, and a new thought is provided for improving the immunity; the prepared active collagen peptide can significantly improve the proliferative activity, phagocytic ability and NO secretion amount of RAW264.7 cells and change the cell morphology, has an important regulation effect on immune balance recovery of immunosuppression model mice, and can regulate the problem of low immunologic function of mice induced by cyclophosphamide, regulate immune balance and improve the body immunity.

The present invention relates to a bispecific chimeric antigen receptor targeting CD117 and CD7 and its application, wherein the bispecific chimeric antigen receptor comprises a signal peptide, an antigen binding domain, a hinge region, a transmembrane domain and a signal transduction domain, wherein the antigen binding domain comprises a CD117 binding domain and a CD7 binding domain. The bispecific chimeric antigen receptor provided by the present invention combines targeted antibodies for CD117 and CD7 together, recognizes and binds to two different antigens at the same time, enhances the targeted elimination ability of malignant cells, and by acting on hematopoietic stem cells and immune cells at the same time, can more effectively eliminate tumor cells in the receptor, while retaining or reconstructing normal hematopoietic and immune functions.

The invention provides application of lactobacillus paracasei IOB413 and a metagen thereof in preparation of a medicine for improving radioactive hematopoietic injury, and relates to the field of microorganisms. The lactobacillus paracasei IOB413 is preserved in the China General Microbiological Culture Collection Center on June 29, 2018, and the preservation number of the lactobacillus paracasei IOB413 is CGMCC (China General Microbiological Culture Collection Center) No.16022. The lactobacillus paracasei IOB413 has the advantages that the lactobacillus paracasei IOB413 is preserved in the China General Microbiological Culture Collection Center; the lactobacillus paracasei IOB413 and the metaplasm thereof can improve the damage condition of immune organs, increase the number of lymphocytes, increase the number of red blood cells and blood platelets and recover the immune function and hematopoietic function of the organism, so that the radioactive hematopoietic damage is improved.

The present application relates to a kind of Bifidobacterium longum infantis subsp.Imu-01 and its application. Specifically, the present application provides Bifidobacterium longum infantis subsp.Imu-01, culture, preparation and preparation of immune function improving product application. The present application provides that Bifidobacterium longum infantis can regulate the immune level of the subject, improve the content of intestinal butyric acid, improve the utilization of human milk oligosaccharide.

The invention relates to the technical field of genetic engineering and biological agents, in particular to a hybrid peptide with immunoregulation and anti-oxidation functions and a preparation method and application of the hybrid peptide. The polypeptide provided by the invention has immunoregulation and antioxidation functions at the same time, and in a normal or immunosuppression state, the polypeptide can significantly improve the immunologic function of a body and reduce the damage of immunosuppression to the body; the oxidation resistance of cells and organisms can be enhanced; meanwhile, the polypeptide has the advantages of low cytotoxicity, high safety, simplicity and convenience in preparation and low cost, can be used as an ideal immunomodulator and an antioxidant, is widely applied to the fields of medicines, foods, health care, feeds, nutrition and the like of human and animals, and has very good application potential and value.

The invention provides a novel three-antigen HSV-2 adjuvant subunit vaccine as well as a preparation method and application thereof. The vaccine comprises herpes simplex virus recombinant protein and a composite adjuvant, the herpes simplex virus recombinant protein comprises extracellular domains of three proteins, namely HSV-2gE, gD and gC, wherein the amino acid sequences of the extracellular domains of the HSV-2gE, the gD and the gC are respectively shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3; and the composite adjuvant is composed of CpG oligodeoxynucleotide and QS-21. Experiments prove that by adding the composite adjuvant composed of CpG oligodeoxynucleotide and QS-21, the immunogenicity, in-vivo protectiveness, virus removal and other immune functions of the HSV-2 subunit vaccine can be remarkably improved; meanwhile, the vaccine is a cross immune protection vaccine of an HSV-2 virus strain and an HSV-1 virus strain.

The invention discloses bifidobacterium breve BBr26 capable of inhibiting hyaluronidase activity and relieving allergy as well as an application, a product and a method of the bifidobacterium breve BBr26, and belongs to the technical field of microorganisms. The invention provides a Bifidobacterium breve (Bifidobacterium breve) strain BBr26 with anti-allergy and / or immunoregulation capability, and the preservation number of the Bifidobacterium breve strain BBr26 is CGMCC (China General Microbiological Culture Collection Center) NO.32424. The invention also provides a preparation method of the Bifidobacterium breve strain The invention also provides an application of the strain bifidobacterium breve BBr26 in preparation of products for relieving atopic dermatitis, and / or food for enhancing immune function, and / or bacteriostatic agents, and a product and a method based on the strain BBr26. The strain disclosed by the invention has broad-spectrum bacteriostasis, can relieve allergy and atopic dermatitis, and can effectively play a role in immunoregulation.

The invention provides a strain for enhancing an immune function and application thereof, and belongs to the technical field of microbial medical treatment. The fungus provided by the invention is cordyceps sinensis qinggensis Cs-2025, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO.41879. The invention also provides a preparation method of the cordyceps sinensis qinggensis. The strain provided by the invention can be used as a substitute of cordyceps sinensis, has the effect of remarkably enhancing the immune function, and is free of toxic and side effects. The method disclosed by the invention is low in production cost, short in time and easy for large-scale production, and an effective way is provided for solving the problem of shortage of natural cordyceps sinensis resources.

The invention discloses a method for generating a personalized vaccination scheme of a rabies vaccine in combination with an electronic medical record, and relates to the technical field of intelligent vaccination management, and the method comprises the following steps: extracting all structured feature information and unstructured feature information related to immune function evaluation in the electronic medical record of a patient, and constructing a feature data original mapping table; according to the method, by constructing the feature mapping table, the conflict relation matrix and the propagation path diagram, the logic conflict of the immune related information in the electronic medical record is accurately identified, the conflict intensity is quantified, the risk index is generated, and then the inoculation strategy path of the main feature and the alternative feature is dynamically adjusted. According to the method, the adaptability and fault-tolerant ability of the system to multi-source heterogeneous medical data are improved, decision deviation caused by feature conflicts is effectively avoided, the scientificity and safety of a personalized vaccination scheme of the rabies vaccine are enhanced, and the method has remarkable clinical and public health values.

The invention discloses bifidobacterium longum subsp. Infantis BIN31 for regulating immunity as well as application, a product and a method thereof, and belongs to the technical field of microorganisms. The invention provides a bifidobacterium longum subsp. Infantis BIN31, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No. 33422, and the preservation number of the bifidobacterium longum subsp. Infantis BIN31 is CGMCC No. 33422. The invention also provides an application of the strain in preparation of food for enhancing immune function, and / or food with an auxiliary protection function on intestinal barrier, and / or food for regulating intestinal flora function, and / or a bacteriostatic agent, and also provides an application of the strain in preparation of food for enhancing immune function, and / or food with an auxiliary protection function on intestinal barrier, and / or food for regulating intestinal flora function. The invention also discloses a bacteriostatic agent and an in-vitro bacteriostatic method based on the strain, which have the functions of enhancing immune function, assisting in protecting intestinal barriers and regulating intestinal flora. The bifidobacterium longum subsp. Infantis BIN31 disclosed by the invention can effectively assist in protecting intestinal barriers, regulating intestinal flora, enhancing immunity and realizing broad-spectrum bacteriostasis.

The invention relates to the technical field of food, medicine and health care products, and discloses a composition containing rosemary exosome vesicles and application of the composition in anti-inflammatory, the composition is formed by mixing plant cell-derived exosome vesicles and functional active ingredients, the composition comprises the following components in percentage by mass: 45-55% of rosemary exosome and 45-55% of turmeric exosome; the preparation method of the composition containing the rosemary exosome vesicles comprises the following steps: S1, taking a certain amount of fresh rosemary and turmeric plants, removing root hairs and impurities, washing the fresh plants with domestic drinking water, washing the fresh plants with purified water for three times, and finally washing the fresh plants with ultrapure water for three times for 3-5 minutes each time; the product can carry various bioactive molecules, transfer cell signals in a transboundary manner, participate in anti-inflammation, inhibit tumor cell proliferation and promote normal tissue repair, has an anti-inflammatory effect, inhibits release of inflammatory factors, improves the immune function of a body and adjusts the immune balance state, and can play a role in neovascularization diseases of the body.