191 results about "Immunologic function" patented technology

The present application belongs to the field of aquaculture technology, and mainly relates to a traditional Chinese medicine composition for preventing and treating acute hepatopancreas necrosis disease of prawn, a preparation method and application. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 28-32 parts of Radix Sanguisorbae, 28-32 parts of Radix Scutellariae, 18-22 parts of Phellodendri Cortex and 8-12 parts of Lonicerae Japonicae Flos. The traditional Chinese medicine composition can significantly inhibit the activity of the main pathogen of the disease, i.e. Vibrio parahaemolyticus, improve the immune function and disease resistance of prawn, and further repair the hepatopancreas and intestinal tissues of the diseased prawn, thereby achieving the effect of effectively preventing and treating the acute hepatopancreas necrosis disease of prawn.

The invention relates to the field and discloses application of terahertz waves or intermediate infrared in preparation of adoptive T lymphocytes. The Terahertz wave or mid-infrared modulation technology is utilized to specifically regulate and control the functions of the T cells in a flowing irradiation mode, and the functions of the T cells from different sources are enhanced under the conditions that no medicine is used and no exogenous gene is introduced. Terahertz wave or mid-infrared stimulation is applied to the T cells in a flowing irradiation mode, specific terahertz wave or mid-infrared frequency for remarkably enhancing the functions of the T cells is determined in a frequency screening mode, and after the optimal irradiation frequency is determined, the T cells with the most remarkable function improvement are further input back into a donor. The T cells stimulated by terahertz waves or mid-infrared rays can play a powerful immune function in a tumor microenvironment so as to inhibit further development of tumors.

The invention relates to the technical field of bioengineering, in particular to a preparation method of a probiotic and exosome preparation applied to immunoregulation. The probiotics and the umbilical cord stem cell exosome are combined for use, the immune regulation effect is enhanced from multiple targets and multiple ways, the immune function of the body is improved, the exosome carries a specific molecular marker and acts on immune cells in a targeted mode, precise immune regulation is achieved, and the preparation is in an oral form and convenient to use; the exosome is extracted from umbilical cord stem cells, has no pathogenicity and immunogenicity, and is low in adverse reaction risk after being used for a long time, and the prepared combined preparation can be used for treating and preventing various immune-related diseases, and also can be used for daily conditioning of people with low immunity and improving the body resistance; meanwhile, the combined preparation adopts micro-capsule embedding, so that the probiotics can be effectively protected from gastric acid and bile erosion, the survival rate and colonization efficiency of the probiotics in intestinal tracts are improved, the immunoregulation effect is further enhanced, and the combined preparation is convenient to store and transport and has a good application prospect.

The invention discloses an immune homeostasis supporting agent based on mushroom active ingredients and fungal polysaccharides, and relates to the technical field of biological medicine and functional foods.The method comprises the following steps that a pleurotus citrinopileatus homeostasis adjusting extracting solution is prepared, specifically, pleurotus citrinopileatus sporocarp powder is extracted with water at the temperature of 80-100 DEG C according to the material-liquid ratio of 1 g: 35-45 mL, and a pleurotus citrinopileatus homeostasis adjusting extracting solution is obtained; the leaching time is 30-60 minutes, so as to obtain pleurotus citrinopileatus leaching liquor; the pleurotus citrinopileatus leach liquor is sequentially subjected to vacuum concentration and decoloration treatment, a pleurotus citrinopileatus steady-state regulation extracting solution with the concentration being 0.5-2 g / mL is obtained, and the pleurotus citrinopileatus steady-state regulation extracting solution shows the two-way regulation characteristic in an experimental model, so that the immunologic function index in the immunosuppression state can be improved, the excessive immune response can be relieved, and the immunologic function index of the pleurotus citrinopileatus can be improved. Therefore, the immune system is guided and maintained in a balanced and stable optimal state.

Provided are a postbiotic of Weizmannia coagulans, and a preparation method therefor, and a use thereof. The postbiotic comprises inactivated bacteria and / or lipoteichoic acid. The postbiotic can enhance the phagocytosis activity of macrophages, the cytokine and NO secretion ability of macrophages, and the expression levels of immune-function-related genes and proteins, and has an effect of inducing human PBMC cells to kill cancer cells.

The invention relates to the technical field of immune carriers, in particular to immune carrier microspheres loaded with individualized MHC-II binding polypeptide and vaccine preparation and application of the immune carrier microspheres loaded with the individualized MHC-II binding polypeptide. The core microsphere is loaded with individualized MHC-II binding polypeptide, the sequence of the MHC-II binding polypeptide is obtained by predicting and screening based on an HLA genotyping result, and each HLA allele corresponds to at least one high-affinity MHC-II binding polypeptide; and the shell is a glucan or other polymer coating layer. The preparation method has the advantages that the T epitope and the B epitope are separately subjected to immune competitive inhibition inside and outside the microspheres, so that a better immune effect is obtained. The antibody avoids cross reaction side effects; carrier molecule diversity is reduced, and side effects caused by T cell over-activation are avoided; th1 epitopes and Th2 epitopes can be contained in the microspheres, so that antibody immunity and T cell immunity functions are generated; the particle size of the microspheres is controllable, and the immunologic function can be achieved without adjuvants.

The invention provides the food calling type river crab feed, the application effect of the food calling type river crab feed in river crab culture shows that the food calling type river crab feed has remarkable advantages in the aspects of improving the growth performance, enhancing the immune function, optimizing the intestinal microecology, improving the water quality environment and the like, the use of the fish meal is reduced through the fermented vegetable protein, and the specific composite phagostimulant has the advantages that the utilization rate of the fish meal is reduced; the palatability of the river crab feed with the fish meal content reduced is improved, the feed coefficient is reduced, the unit economic benefit is improved, the problem of environmental pollution in the breeding process is effectively relieved, and the river crab feed has good ecological and economic dual values.

The present application belongs to the technical field of animal health care, and particularly relates to a fermented decoction for improving growth and immune function of broilers and a preparation method thereof. The fermented decoction for improving growth and immune function of broilers is prepared from the following raw materials by fermentation according to weight parts: radix codonopsis 8-12 parts, angelica sinensis 3-7 parts, cornus officinalis 8-12 parts, cinnamomum cassia 3-7 parts and portulaca oleracea 3-7 parts. The fermented decoction significantly increases the body weight of broilers, significantly improves the thymus index and spleen index, and increases the IgG and IgM contents in serum, thereby promoting the growth of broilers and improving the immune function of broilers. The preparation method of the fermented decoction is simple, and is suitable for industrialized production and market promotion and application.

The invention discloses a method for improving the quality of ganoderma lucidum mushroom residues through synergistic fermentation of bacteria and enzymes and application of the method. According to the method disclosed by the invention, cellulase, beta-galactosidase and xylanase with proper concentrations and lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae with proper proportions are adopted, and enzymes and bacteria are mutually cooperated, so that the characteristics of the raw materials are well balanced, the finally prepared ganoderma lucidum mushroom bran is high in protein content and low in fiber content, and the nutritional value of a feed is improved; the total amount of amino acids and essential amino acids are remarkably increased, and the feed protein quality can be improved; the rumen dry matter digestibility and energy metabolites are remarkably improved, and ruminant absorption and utilization are facilitated; the ganoderma lucidum mushroom bran fermented feed is added into the daily ration, so that the daily gain, the carcass rate and the dressing percentage of fed animals can be remarkably improved, the levels of serum GLU, TG, ALT and AST are reduced, the expression of key inflammatory cytokines (IL-1beta and IL-6) is inhibited, and the ganoderma lucidum mushroom bran fermented feed has the comprehensive effects of regulating glycolipid metabolism and relieving liver load and systemic inflammation, so that the immune function of animal bodies is improved.

The invention discloses an oyster-derived polypeptide and application thereof, and belongs to the technical field of bioactive peptides. The oyster source polypeptide provided by the invention is selected from at least one of the following polypeptide sequences: PAPLWQMPKFRN, NPVDPAPLWQMPK, PFPL, VPPF, PLNF, APAPAVKYLR, PPMIFKTR, PPMIFKTRIM, AVNMVPFPR, FSLP, PFKP, FPSIVGRPR, VPKPYLNHPL, LPPL, PQDVPPKKPLIMIAAR, VPFVPYLNHPL, FLLK, SPPELPDVM and MPVTDPPLRTF. The invention also discloses a preparation method of the oyster source polypeptide. The invention also discloses application of the oyster-derived polypeptide in preparation of food, health care products or medicines with an immune regulation function. The oyster source peptide product disclosed by the invention has digestion stability and immunocompetence, can keep a basic structure after simulating gastric juice digestion, and can activate the immune function of RAW264.7 cells and improve the expression quantity of cell factors. The method provided by the invention can provide a new direction for high-value utilization of marine resources, and has a good application prospect.

The invention relates to the technical field of polypeptide compositions, and discloses a silkworm-derived protein peptide with blood sugar regulation and immune function activity and an application method, the silkworm-derived protein peptide comprises a silkworm-derived acidic active peptide group, a silkworm-derived alkaline daemon peptide group, a divalent cation coordination center and a silkworm-derived proline enriched peptide group; the silkworm source acidic active peptide group and the silkworm source alkaline daemon peptide group are mixed according to the mass ratio of (3: 1)-(5: 1), and an electrically neutral nano peptide cluster with the average particle size smaller than 100 nm is formed through electrostatic complexation; the silkworm-derived alkaline daemon peptide group contains histidine residues, imidazolyl of the silkworm-derived alkaline daemon peptide group is protonated in an acid environment to generate charge repulsive force, electrostatic repulsive force generated when active peptide penetrates through intestinal epithelial cell membranes is eliminated by utilizing a component charge counteracting mechanism, the apparent treatment flux of transporter protein on core components is enhanced, and the biological activity of the silkworm-derived alkaline daemon peptide group is improved. And intracellular dissociation of the peptide cluster is triggered by virtue of a protonation induction lock, so that accurate resetting of an active site at a target spot is ensured, and the technical problems that transmembrane absorption of the high-activity polar peptide is limited and intracellular release is delayed are solved.

This invention discloses a composition for enhancing immunity through gut microbiota, its preparation method, and its application. The composition comprises the following raw materials: a complex prebiotic, polyphenols, inactivated *Saccharomyces boulardii*, and plant extracts. The complex prebiotic includes human milk oligosaccharides and arabinogalactan, the polyphenols include berry polyphenols and grape polyphenols, and the plant extracts include pomegranate seed extract and kale extract. This invention's composition, on the one hand, selectively enriches *A. AKK* bacteria through prebiotics; *A. AKK* can directly interact with various immune cells to enhance immunity. On the other hand, supplementing with yeast factors can also inhibit inflammation and enhance immune responses, thereby significantly increasing the number of *A. AKK* bacteria in the gut microbiota and simultaneously improving intestinal cellular immune function.

The present invention relates to an oncolytic adenovirus capable of co-expressing interleukin-12 and a C-met-inhibiting oligonucleotide; and an antitumor immune-boosting composition and anticancer composition comprising the oncolytic adenovirus. The present invention has first identified an adenovirus system having simultaneous effects of IL-12 expression and C-met inhibition in cancer gene treatment. The adenovirus system of the present invention is capable of inhibiting C-met while expressing interleukin-12, thereby restoring immune functions in a tumor environment to enhance anticancer effects such as the inhibition of tumor recurrence and tumor growth and to inhibit tumor migration. Accordingly, the adenovirus system of the present invention can be effectively used in the treatment of cancer.

The present application relates to the technical field of microorganisms, and particularly relates to a bifidobacterium BGLP1 capable of regulating sugar digestion and high GLP-1 production and application thereof. Bifidobacterium bifidum The bifidobacterium BGLP1 (CGMCC No. 35718) has the abilities of inhibiting alpha-glucosidase and alpha-amylase, stimulating STC-1 cells to secrete GLP-1 and activating the intestinal glucose-lowering pathway, significantly inhibiting pancreatic lipase activity and effectively reducing dietary fat absorption, converting excess sucrose into extracellular polysaccharides to increase the probiotic function, promoting the conversion of white fat into brown fat, and activating macrophages and improving the immune function of the body. In addition, the bifidobacterium BGLP1 has strong intestinal colonization ability, has good tolerance in artificial gastric juice and artificial intestinal juice, and can successfully reach the human intestinal tract.

This invention discloses a traditional Chinese medicine composition, preparation, and its application in the preparation of a drug for treating postoperative cancer-related fatigue in thyroid cancer patients. The traditional Chinese medicine composition comprises the following components in parts by weight: 30-50 parts raw Astragalus membranaceus, 5-20 parts stir-fried Atractylodes macrocephala, 5-20 parts Curculigo orchioides, 5-20 parts Epimedium brevicornu, 5-20 parts Prunella vulgaris, 5-20 parts Dendrobium nobile, and 5-20 parts Lycium barbarum. This invention combines raw Astragalus membranaceus, stir-fried Atractylodes macrocephala, Curculigo orchioides, Epimedium brevicornu, Prunella vulgaris, Dendrobium nobile, and Lycium barbarum. Through the rational combination of these raw materials, it can effectively improve immune function, relieve physical and mental fatigue, and improve the quality of life of postoperative thyroid cancer patients.

The application discloses application of COPG1 protein in preparation of a drug for promoting M2 polarization of macrophages, and belongs to the technical field of biological medicines.The application firstly proposes that COPG1 can promote M2 polarization of macrophages, and that COPG1 in tumor-derived exosomes can inhibit the M2 polarization of macrophages.The strategy of regulating the COPG1 content in macrophages and exosomes and inhibiting the M2 polarization of macrophages to regulate the immune function of macrophages proposed by the application provides a theoretical and practical basis for developing an immune regulator by targeting COPG1.

The application discloses a spleen-targeting nanoassemblies of active ingredients of Sanhuang Xiexin Decoction, a preparation method and application thereof, and belongs to the technical field of biological medicine, and comprises spleen-targeting exosomes and nanoassemblies containing active ingredients of Sanhuang Xiexin Decoction, wherein the nanoassemblies containing active ingredients of Sanhuang Xiexin Decoction are loaded on the spleen-targeting exosomes; and the active ingredients of Sanhuang Xiexin Decoction are three active ingredients corresponding to three traditional Chinese medicines, i.e., rhubarb, Coptis chinensis and Scutellaria baicalensis. The application has the beneficial effects that based on three key active components of Sanhuang Xiexin Decoction, the nano delivery system of the carrier-free pure medicine of three active ingredients of traditional Chinese medicine compound is developed for the first time through a chemical self-assembly technology, support is provided for the development of a new dosage form of the nano preparation of the traditional Chinese medicine compound, and the spleen immune function disorder-mediated diseases have a good targeted treatment effect.

The application discloses an immunoregulatory antibacterial peptide targeting LPS and a preparation method and application thereof, and belongs to the technical field of biology. The amino acid sequence of the antibacterial peptide FF2 is shown as SEQ ID No. 1, and the antibacterial peptide FF2 of the application can specifically target the bacterial membrane surface component LPS to make the bacterial membrane leak and inhibit the growth of bacteria. The antibacterial peptide can capture bacteria and trigger the innate immune system to achieve the antibacterial purpose, and can also target the bacterial membrane to break the membrane and kill bacteria, thereby fully avoiding the killing of beneficial bacteria in the host body, avoiding the overuse of the antibacterial peptide, effectively reducing the generation of drug resistance, and mobilizing the immune function of the body to synergistically resist bacterial infection. In conclusion, the antibacterial peptide FF2 is a targeted LPS forming nanofiber antibacterial peptide with immunoregulatory function, which has great development potential and high application value.

The application provides an animal immune evaluation method based on a Lutzomyia longipalpis infection model, and comprises the following steps: S1, experimental animal preparation and screening; S2, Lutzomyia longipalpis preparation and quality verification; S3, Lutzomyia longipalpis infection of experimental animals; S4, immune related index detection; S5, animal immune function evaluation; and S6, repeatability verification and data summarization. The application realizes systematic and objective evaluation of the effect of immune candidate drugs.

The present invention relates to an animal model for research on cancer immunotherapy agents, and a preparation method therefor. A nude mouse, which is a mouse model widely used for evaluating the effects of anticancer agents, can verify the efficacy of a cytotoxic anticancer agent, but cannot confirm the effect of an cancer immunotherapy agent because immune function is deficient. However, a cancer animal model according to the present invention has normal immune function, thereby enabling preclinical efficacy of a cancer immunotherapy agent to be accurately predicted, and thus is expected to contribute to improving the prognosis of a patient for whom surgery is not possible.

The application relates to application of a reagent for detecting expression levels of a PSIP1 gene or a coded protein LEDGF / p75 in preparation of a product for evaluating systemic lupus erythematosus disease activity, which makes up for the problems of insufficient sensitivity and specificity of existing evaluation indexes; the application also discloses a key role of the PSIP1 gene in a pathogenesis mechanism of systemic lupus erythematosus, and clearly shows the relationship between the PSIP1 gene in T cells and cell cycle arrest, cell aging and abnormal immune function, so as to provide a theoretical basis for target regulation of the PSIP1 to improve T cell function and delay or block disease progression; the application firstly proposes that the expression levels of the PSIP1 / LEDGF / p75 are detected to be used for evaluating systemic lupus erythematosus disease activity, auxiliary diagnosis, prognosis prediction and treatment effect monitoring, so as to provide a brand-new tool and direction for precise diagnosis, activity evaluation, prognosis judgment and future target treatment development of systemic lupus erythematosus.

The invention provides an application of OICR-9429 or a pharmaceutically acceptable salt thereof in preparation of a product for improving senescence indications. The OICR-9429 provided by the invention shows multiple beneficial technical effects in the aspect of improving senescence-related indications, not only can effectively relieve inflammatory response caused by pyroptosis in the senescence process, but also can improve the immune function and anti-infection ability of senescence organisms. The OICR-9429 also has a remarkable treatment effect on sepsis, the increase of the inflammation level caused by infection is inhibited, the survival rate of old patients is increased, and the prognosis is improved. Long-term intervention of the OICR-9429 can continuously relieve the chronic inflammation state of an aging body, increase the muscle mass, enhance the exercise ability, prolong the life and reduce various lesions accompanied by aging. The invention lays a foundation for developing products for comprehensively intervening aging and related function decline, and has important application value in the aspect of improving the life quality of old people.

Methods of inoculating an animal with a non-cultivable spore-forming bacteria, such as segmented filamentous bacteria (SFB), are provided. Also provided are methods of preparing a litter enriched with the bacteria, as are methods of using the enriched litter to inoculate animals with the bacteria. The disclosure further relates to the use of non-cultivable spore-forming bacteria to improve gastrointestinal health, reduce bacterial pathogens, and stimulate host immune function in animals.

The invention belongs to the field of feed additives, and particularly relates to a nano feed additive for improving intestinal flora and a preparation method thereof.The preparation method comprises the following steps that (1) an electric arc furnace with pure copper as an anode and a graphite rod as a cathode is provided, absolute ethyl alcohol is injected into the furnace body in the vacuum state in the electric arc furnace, the arc starting current is controlled to be 40-60 A, and the temperature is controlled to be 20-30 DEG C; and purifying in an argon atmosphere for 12-48 hours to obtain the Cu-coated C nano-particles. And (2) carrying out oxidation reaction on the Cu-coated C nano-particles in an oxygen-containing atmosphere at the reaction temperature of 200-350 DEG C for 1-5 hours to obtain an oxidized product, namely the nano feed additive. The nano feed additive disclosed by the invention can be used for preventing and treating bacterial infection, enhancing the immune function and the anti-oxidative stress function of an organism, improving intestinal flora and prolonging the service life of animals, is an effective and safe functional feed additive, is simple in preparation method and low in production cost, can realize large-scale preparation, and has an important application prospect.

The invention discloses a pharmaceutical composition for treating psoriasis and application thereof, and belongs to the technical field of traditional Chinese medicines. The pharmaceutical composition is prepared from the following raw materials in parts by weight: 12 to 35 parts of radix isatidis, 12 to 20 parts of sophora flower bud, 8 to 18 parts of radix sophorae flavescentis, 8 to 18 parts of radix salviae miltiorrhizae, 8 to 15 parts of radix angelicae sinensis, 8 to 18 parts of herba houttuyniae, 8 to 15 parts of solanum septemlobum bunge, 8 to 18 parts of caulis spatholobi, 8 to 18 parts of radix astragali seu hedysari and 4 to 8 parts of radix et rhizoma rhei. The pharmaceutical composition disclosed by the invention has a remarkable curative effect on psoriasis vulgaris, can effectively relieve skin lesion symptoms of patients, alleviate the inflammation level of organisms and regulate the immune function of the organisms, and has important significance on improvement of prognosis conditions of the patients.

The application discloses a caviar polypeptide composition with enhanced immune function, a preparation method, application and preparation, belongs to the technical field of bioactive polypeptides, and comprises at least one polypeptide with an amino acid sequence of RWFPGKPLFWRA, FWKPLRGALFPW or KWRFPGALPWFA and a molecular weight of 1400-1550 Da. The preparation method comprises caviar protein extraction, complex enzymolysis, ultrafiltration separation, chromatography purification and solid-phase synthesis. Animal experiments prove that the polypeptide can significantly enhance cell immunity, humoral immunity, monocyte-macrophage function and NK cell activity, the mechanism is related to TLR2 / TLR4 receptor activation and NF-κB / MAPK signal pathways, the safety is good, and the polypeptide is suitable for development into an immune-enhancing health food or medicine.

The invention discloses a feed additive for replacing antibiotics to treat chicken necrotic enteritis and application of the feed additive, and belongs to the technical field of feed. The effective component of the feed additive is the tannin extract from chestnut trees, and animal experiments prove that the tannin extract from chestnut trees can improve the production performance of broiler chickens with necrotic enteritis to a certain extent, improve the intestinal morphology and immune function of the broiler chickens with necrotic enteritis and improve the immunity of the broiler chickens with necrotic enteritis. Therefore, the chestnut tree tannin extract can replace antibiotics and has a good treatment effect on broiler intestinal inflammation caused by clostridium perfringens and coccidiosis.

The invention discloses application of a molecular genetic marker in simultaneous prediction of intestinal immunity, egg weight at first laying and body weight at first laying. The molecular genetic marker disclosed by the invention can be used for simultaneously marking the intestinal immune function, the egg weight at the first laying time and the body weight at the first laying time; by means of the detection method, one-time detection can be achieved, and early, accurate and efficient prediction of the intestinal immune function, the first laying weight and the first laying egg weight of the chickens can be completed. The'one-point three-detection 'technology accords with the mainstream goal of laying hen breeding, and can save the detection cost: taking a single sample detection condition as an example, under the detection method of the same type of PCR principle, corresponding sites are respectively detected for three times if the three characters are detected, however, the prediction of the three characters can be realized by only detecting for one time in the invention, and the detection cost is greatly reduced. The detection cost is reduced by 2 / 3, and meanwhile, the important characters of a plurality of chickens can be predicted.

The application discloses a composite adjuvant containing CpG oligodeoxynucleotide and aluminum adjuvant, and a new coronavirus COVID-19 inactivated vaccine using the composite adjuvant. The CpG oligodeoxynucleotide is used in combination with the aluminum adjuvant, and a strong immune enhancement effect can be generated. When the composite adjuvant is applied to the new coronavirus COVID-19 inactivated vaccine, the residence time of the antigen in the body can be greatly prolonged, and the hydrolysis of the antigen by various hydrolytic enzymes is reduced. The preparation method of the new coronavirus COVID-19 inactivated vaccine provided by the application is simple, the quality is easy to control, the vaccine is easy to mass-produce, has good safety, low toxic and side effects, and is particularly suitable for being used for the prevention and treatment of the new coronavirus COVID-19 in various populations, including the low-immunity population such as the middle-aged and the old.

The present invention provides a method of treating high-grade serous cancer in a patient in need thereof, comprising administering to the patient in need thereof an effective amount of an SIK2 / 3 inhibitor or a pharmaceutically acceptable salt thereof, where the effective amount is lower than the amount required to directly kill tumor cells. The method of treating high-grade serous cancer in immune-functioning patients in need thereof may also include administering an immune checkpoint inhibitor.