178 results about "Organ damage" patented technology

The invention relates to the technical field of microorganisms, and particularly discloses a fermentation method of bifidobacterium longum BBMN68 as well as a fermented product, a postbiotic and application of the fermented product. According to the method for fermenting the bifidobacterium longum BBMN68, a fermentation substrate comprises alkaline protease hydrolyzed milk and a pomelo peel extract; the hydrolysis rate of the alkaline protease hydrolyzed milk is 9-11%, and the water-soluble dietary fiber content of the shaddock peel extract is 75-80%; the mass ratio of the alkaline protease hydrolyzed milk to the shaddock peel extract is (10-15): (1-3). The content of active ingredients in the fermented product prepared by the method is high, and after the fermented product is prepared into the postbiotics to be taken, the immune function of an organism can be recovered, immune organ damage is reduced, inflammation is relieved, and intestinal flora is regulated and controlled.

The invention discloses a sepsis patient organ function damage early warning judgment method based on machine learning, and the method comprises the steps: obtaining the multi-modal clinical data of a sepsis patient, including vital sign time sequence data, inspection data, treatment intervention data and static patient basic information; after data standardization processing, organ function associated features are extracted through a multi-scale feature fusion strategy, and an organ-level feature set is constructed; the method comprises the following steps of: obtaining a multi-organ collaborative early warning model, inputting the multi-organ collaborative early warning model into a pre-trained multi-organ collaborative early warning model, respectively constructing an inter-organ compensatory relation map by double branches of the model, quantifying organ injury risk contribution degree, and outputting a multi-organ functional injury risk matrix by combining with attention mechanism weighted fusion; and generating an early warning result based on the risk matrix and the organ specificity early warning threshold, including the injury risk level of each organ and the dominant risk key feature identifier, monitoring data update in real time, and dynamically adjusting the interval to update the early warning result. According to the invention, the limitation of traditional single-source data and single-organ early warning is broken through, and the early warning accuracy and real-time performance are improved.

The present disclosure relates to certain compositions, kits, devices, systems and methods, e.g., compositions, kits, devices, systems and methods for assessing methylation status, and / or assessing tissue and / or organ damage or toxicity in a subject. In particular aspects, provided herein are compositions, kits, devices, systems and methods for assessing tissue and / or organ damage or toxicity in a subject based on assessing methylation status of selected one or more tissue-specific or organ-specific target polynucleotide(s), e.g., one or more tissue-specific or organ-specific target genomic DNA sequence(s), from the subject.

The invention discloses a method for evaluating human health by airborne pollutants, and belongs to the technical field of pollutant evaluation, and the method comprises the following steps: S1, simulating a real exposure environment, constructing an airborne pollutant exposure simulation system, S2, systematically monitoring multiple organ indexes, and systematically monitoring pollutants, and S3, carrying out interdisciplinary comprehensive analysis and evaluation. A research team formed by environmental science, medicine and molecular biology multidisciplinary professionals is established, key organ damage is analyzed, the influence of pollutants on human health is comprehensively evaluated, the research design is close to the real environment by simulating the real exposure environment, the whole process of the pollutants from exposure to excretion can be simulated, and the real exposure environment is simulated. A scientific basis is provided for evaluating the health risk of the pollutants, meanwhile, through the propagation path of the pollutants in the human body, multiple organ damages are connected in series, a systematic research framework is formed, and the limitation of single organ analysis in traditional research is avoided.

The invention discloses a preparation method of a cochlear organ chip integrated with a blood labyrinth barrier, and belongs to the field of biomedical engineering. The preparation method of the simulated blood labyrinth barrier comprises the following steps: constructing an endothelial cell culture cavity and a pericyte culture cavity, and separating the endothelial cell culture cavity and the pericyte culture cavity through a simulated basement membrane; and respectively inoculating percutaneous cells and endothelial cells into a percutaneous cell culture cavity and an endothelial cell culture cavity, and culturing to obtain the simulated blood lost barrier. The chip can effectively simulate the structure and the function of the BLB, and the barrier integrity of the BLB is evaluated through TEER (trans-epithelial electrical resistance) measurement and an apparent permeability coefficient. The invention also relates to application of the chip in NIHL drug screening, in particular to drug evaluation for oxidative stress induced inner ear organ injury. Through the platform, the protection effect of the candidate drugs on the TBHP-induced oxidative stress injury of the inner ear organs can be evaluated, and a new tool and thought are provided for developing novel NIHL treatment drugs.

The invention discloses a composition for preventing and treating plateau erythrocythemia and application of the composition, and relates to the technical field of pharmaceutical composition preparation. The composition is prepared from PT2385 and salidroside. The combination of PT2385 and salidroside can effectively inhibit hyperplasia of red blood cells of HAPC rats, reduce hemoglobin and relieve blood flow stasis and microcirculation disturbance of body tissues and organs caused by hypoxia, so that hypoxia phenotypic characteristics of the HAPC rats are improved. In addition, free radicals can be effectively removed, oxidative stress, peroxidation reaction and inflammatory reaction induced by hypoxia are inhibited, the mitochondrial function is protected, and the dysfunction of HAPC rat tissue and organ injury is improved. The problem that in the prior art, an effective medicine for preventing and treating plateau erythrocythemia is lacked is solved.

The invention relates to an application of a bioactive peptide YPFPGPIH derived from bovine casein in the aspect of anti-inflammation or immunoregulation. The peptide can be combined with TLR2 and TLR4 to inhibit the activation of NF-kappa B and MAPK signal channels, so that the levels of inflammatory factors such as IL-1beta, TNF-alpha and NO are remarkably reduced, the expression of IL-10 is up-regulated, and inflammation balance regulation is realized; the body inflammatory response is improved, and the immune organ injury is recovered. The peptide can be used for preparing anti-inflammatory drugs, immunomodulatory functional foods and nutritional supplements, and has good safety and application prospects.

The invention relates to the technical field of clinical decision support and medical data intelligent analysis, in particular to a critical patient individualized liquid resuscitation decision support system, which is characterized in that a data acquisition unit acquires pulse pressure variation waveform and multi-dimensional physiological data of each stroke volume; a volume benefit analysis channel of the dynamic decision-making unit segments a respiratory cycle, extracts pulse pressure fluctuation characteristics, outputs a per-stroke volume increase probability value through a machine learning classifier, an organ injury early warning channel calculates a blood lactic acid and creatinine clearance rate change rate, and generates an acute kidney injury risk sign in combination with inferior vena cava image verification; the execution control unit sends an infusion control or limit instruction according to the combined state; and the feedback optimization unit adjusts the weight of the model according to the actual value of blood lactic acid removal, and recalculates the probability value, so that individualized precise resuscitation, balance capacity benefit and organ protection are realized, and the treatment safety and effectiveness are improved.

The invention discloses a curcumin nanocrystal preparation as well as a preparation method and application thereof. The invention relates to a curcumin nanocrystal composition which is prepared by drying and curing a curcumin nanocrystal suspension to a blank pellet core, the curcumin nanocrystal suspension contains 20-35% of curcumin nanocrystals, 1-10% of a three-dimensional protective agent and 1-10% of a charge protective agent, and the average particle size of the curcumin nanocrystals is 200-320 nm. The curcumin nanocrystal composition prepared by the invention can effectively prevent and treat heat stroke and complications thereof, remarkably improve heat tolerance, prolong survival time, reduce the level of inflammatory factors in blood, protect intestinal epithelial cells and intestinal barrier functions, reduce damage caused by heat stroke, prevent and treat body and organ damage caused by heat stroke, and improve the curative effect of the curcumin nanocrystal composition. The preparation has the advantages of being high in drug loading capacity, good in stability, high in bioavailability, safe, effective, convenient to carry and the like.

This invention discloses an antigen and vaccine preparation based on dominant Brucella epitopes, and their application, belonging to the field of recombinant subunit vaccine technology. The antigen is composed of CTL epitopes, HTL epitopes, and B-cell epitopes tandemly, and its amino acid sequence is shown in SEQ ID NO.1. Based on screened dominant CTL epitopes, HTL epitopes, and B-cell epitopes of the Omp25 and Omp31 outer membrane proteins, this invention constructs a novel antigen fusion polypeptide. The vaccine prepared based on this polypeptide effectively activates a significant dual immune response, enhancing cellular immunity while inducing the body to produce high levels of specific antibodies. It also provides good protection against organ damage caused by bacterial infection, offering better immune response and protective efficacy against Brucella, laying the foundation for the development of Brucella recombinant subunit vaccines.

The invention discloses application of senkyunolide I in preparation of a medicine for preventing and treating organ injury and / or cytotoxicity induced by cis-platinum, and belongs to the field of medicines, and the mass fraction of the senkyunolide I is greater than or equal to 90%; through a large number of tests, the inventor finds that senkyunolide I can reduce cytotoxicity induced by cis-platinum and improve cell activity; the kidney injury caused by cisplatin chemotherapy is relieved, and the renal tubule injury caused by cisplatin is relieved; the liver injury caused by cisplatin chemotherapy is relieved, the activity of glutamic oxalacetic transaminase and glutamic-pyruvic transaminase induced by cisplatin is reduced, and the degeneration and necrosis of liver cells caused by cisplatin are relieved; moreover, the senkyunolide I can be orally administered, so that the safety risk and adverse reaction caused by the conventional amifostine injection administration can be solved, and the senkyunolide I is expected to be developed into a cisplatin chemotherapy adjuvant drug which can be orally administered.

The present invention relates to methods of producing hypoimmunogenic functional macrophages, and cell compositions comprising the same. The hypoimmunogenic functional macrophages may be polarised or unpolarised. Such cells may be useful in the treatment of diseases, e.g. inflammatory organ damage. The therapy that may be achieved by hypoimmunogenic functional macrophages is universal, and the cells do not require matching from donor to recipient. This broadens the application of cell-based therapies, in particular in patients with chronic liver fibrosis.

The invention discloses application of bacillus subtilis in relieving growth performance reduction and tissue and organ damage of yellow-feathered broilers caused by vomitoxin, and belongs to the field of feed additives. The bacillus subtilis is used for treating the yellow feather broilers eating DON polluted feed, and the bacillus subtilis is found to be capable of remarkably reducing the vomitoxin content in cecum mucosa, cecum content, serum, liver and excrement of the vomitoxin infected yellow feather broilers; the growth performance reduction, liver injury and intestinal injury caused by vomitoxin toxicity of the yellow feather broilers are relieved. The bacillus subtilis plays an important role in early discovery and treatment of yellow feather broilers infected by vomitoxin.

The application provides a preparation method of high-selenium compound powder for postoperative and radiotherapy rehabilitation immune repair, and belongs to the technical field of functional food or special medical use formula food processing technology.The application realizes sufficient wall breaking by respectively pre-wetting and low-temperature complex enzymolysis of hard plant wall and fungal wall raw materials, and then prevents active loss by using endogenous substances of the raw materials for in-situ anchoring without adding, after drying and crushing, adding postbiotic powder, active peptide, whey protein, yeast beta-glucan, EGCG and other heat-sensitive components into low-temperature sterile dry mixing, while maximizing the release of organic selenium and immune active substances, the heat-sensitive functional components are completely protected from degradation and inactivation, and finally the technical target of sufficient wall breaking, complete activity and stable immune repair effect is achieved.The product prepared by the application can improve the immune organ damage and immune homeostasis imbalance caused by radiotherapy, and is suitable for the immune repair of postoperative and radiotherapy rehabilitation population.

The invention is suitable for the technical field of biology, and provides an L-NAME induced trophoblast organ injury model and a construction method and application thereof, and the construction method of the trophoblast organ injury model comprises the following steps: constructing a trophoblast organ by using human villus tissue; the method comprises the following steps: carrying out culture and passage treatment on a trophoblast organ, and then adding L-NAME with the final concentration of 15-60mM to carry out induced injury, so as to obtain the trophoblast organ injury model. The basic model used in the invention is a trophoblast-like organ which can be subcultured for a long time, so that the problems of difficult acquisition of placenta samples and ethics limitation in the clinical early stage are reduced. Meanwhile, L-NAME is used for performing drug-induced injury, a trophoblast organ injury model for simulating the placental pathological state of preeclampsia can be constructed, and compared with traditional hypoxia-induced injury, the method is more convenient, and the limitation of instruments and equipment is reduced.

The invention relates to application of serum mucoid or Tax1 binding protein 1 in preparation of a medicine for treating medicine-induced liver injury or organ ischemia-reperfusion injury. Experiments prove that by using an adeno-associated virus (AAV) carrying an Orm2 gene, ORM2 recombinant protein or knocking down a TAX1BP1 gene, liver injury and liver failure, ischemia reperfusion injury of the liver, ischemia reperfusion injury of the kidney and ischemia reperfusion induction of intestines induced by APAP treatment can be effectively relieved, and the application has the advantages that the liver injury and liver failure, the ischemia reperfusion injury of the kidney and the ischemia reperfusion induction of the intestine can be effectively relieved; and a new target spot and a new treatment method are provided for treating the ferroptosis-related organ injury.

The invention discloses an interpretable machine learning method for predicting multi-organ injuries of diquat poisoning patients, and the method comprises the steps: constructing and verifying a prediction system of eight kinds of organ injuries through a clinical database; toxic encephalopathy, myocardial injury, acute kidney injury, circulatory failure, skeletal muscle injury, liver injury, lung injury and gastrointestinal tract injury are covered. According to the method, early-stage efficient prediction of multi-organ injury is achieved, deep insight is provided for potential biological mechanisms of organ injury, clinical formulation of individualized intervention strategies is facilitated, the prognosis management level of diquat poisoning patients is expected to be remarkably improved, and the incidence rate and case fatality rate of multi-organ failure are reduced. According to the technical scheme, high prediction precision is kept, meanwhile, balance of interpretability, stability and clinical applicability of the model is achieved, a reliable auxiliary diagnosis tool is provided for organ injury risk assessment of acute poisoning patients, and the method has important clinical application value and wide market prospects.

The present disclosure is directed to novel therapeutic approaches for the prevention, treatment and / or delaying progression of chronic injury, progressive loss of functional parenchymal cells, or fibrosis of an organ. Specifically disclosed are agents for use in increasing homodimer-formation of ARNT in an organ in the prevention, treatment and / or delaying progression of chronic injury, progressive loss of functional parenchymal cells, or fibrosis of said organ, as further defined in the claims. In embodiments, said agent may be (i) an inhibitor of protein phosphatase 2A (PP2A) activity, (ii) an inhibitor of the transcriptional repressor complex FKBP12 / YY1, or (iii) an expression construct, which is capable of over-expressing ARNT in said organ, as well as combinations of (i), (ii), and / or (iii).

The invention discloses a novel ferroptosis inhibitor and application thereof in preparation of drugs for preventing and / or treating sepsis, and belongs to the technical field of pharmaceutical preparations and biological medicines. The invention discloses a preparation method of a novel ferroptosis inhibitor, which can be used for developing a high-efficiency and low-toxicity ferroptosis inhibitor. The medicament can be used for preparing a medicament for preventing organ injury related to sepsis, is a potential medicament for treating sepsis, and can inhibit ferroptosis in kidney, liver, lung and cardiomyopathy induced by sepsis and relieve inflammatory response and organ injury. Meanwhile, the invention has the outstanding advantages of strong specificity, high bioavailability, obvious effect and stable preparation method, and has wide application prospects.

The application discloses a positioning method of uterine cardinal ligament and sacral ligament and a pelvic floor finite element evolution model construction method, and belongs to the field of two-dimensional digital modeling and biomechanics research. According to the pelvic floor anatomy structure, the starting point and the ending point of the cardinal ligament and the sacral ligament in the two-dimensional finite element model are determined, and a distributed nonlinear hyperelastic spring group is proposed to simulate the suspension mechanical properties of the cardinal ligament and the sacral ligament. An organ tissue proportional damage simulation method and a proportional damage expression are proposed, the nonlinear material stress-strain curve of the pelvic floor organ tissue is multiplied by a proportional damage factor to simulate the mechanical properties of the pelvic floor organ damage. A concentrated load application method and a concentrated force-displacement expression of the initial shape feature are proposed to simulate the initial genital cleft and the uterine-vaginal angle. The evolution model can intuitively show the mechanical changes and shape feature change trends of the pelvic floor from the initial injury to the severe prolapse, and provides a basis for the reconstruction surgery design of the pelvic organ prolapse.

This invention relates to the field of biomedical technology and discloses an amphiphilic nicotinamide adenine dinucleotide (NAD) + Couplings, nanomicelles and their applications. Amphiphilic NAD+ + Couplings have the following general structural formula: ; where NAD + R is a hydrophilic group, R is a hydrophobic antioxidant group, and phenylboronic acid derivative is the linking bond; R is selected from free radical scavengers, small molecule organoselenic compounds that mimic the function of glutathione peroxidase 4, iron chelating agents, 7-dehydrocholesterol, royal jelly acid, α-lipoic acid, oleic acid, or derivatives of the above molecules. In this invention, NAD... + Conjugates and their self-assembled nanomicelles possess functions such as promoting DNA repair, maintaining cellular homeostasis, and delaying aging. Simultaneously, nanomicelles can inhibit inflammation and oxidative stress, and can physically encapsulate hydrophobic active drugs, showing broad application prospects in inflammatory diseases, age-related diseases, organ damage, and the prevention and treatment of toxic side effects from chemotherapy drugs.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for improving alcohol tolerance of a user and relieving uncomfortable symptoms of the user after drinking. Compared with modern medical treatment, the traditional Chinese medicine compound is started from multiple mechanisms of relieving alcoholism, protecting liver and stomach and dredging collaterals and blood stasis, whole-course intervention from acute symptom control to organ injury repair is achieved, and the traditional Chinese medicine compound is more suitable for the complex pathogenesis of alcoholism multi-system injury. According to the formula, an effective traditional Chinese medicine scheme is provided for drunkenness patients according to the core treatment principle of separately eliminating and draining, and clearing away heat and toxic materials. The composition can significantly improve the alcohol tolerance of a user, alleviate the vomiting symptom of the user after drinking, and improve the discomfort symptom of the user after sobering up. The traditional Chinese medicine composition is a pure traditional Chinese medicine formula, is safe and has no toxic or side effect.

Provided is a pharmaceutical composition and application thereof, specifically, said pharmaceutical composition comprises: salvianolic acid B as a first active ingredient; and ginsenoside Rg1 as a second active ingredient, and the weight ratio of the first active ingredient to the second active ingredient being 5:(1-4.5). The pharmaceutical composition has a better active-ingredient proportion than the prior art, has a better therapeutic efficacy in preventing and / or treating ischemic diseases, and ischemia-reperfusion tissue or organ damage, and can be used for treating ischemia and / or ischemia-reperfusion damage of different tissues and organs.

The invention belongs to the technical field of biological medicine, and provides a human umbilical cord mesenchymal stem cell preparation and application thereof, the preparation comprises human umbilical cord mesenchymal stem cells, ginsenoside Rg3 and a medicinal carrier; the medicinal carrier is a phosphate buffer solution; according to the application, 1 * 10 < 6 > HUCMSCs pretreated by Rg3 are subjected to caudal vein injection on a T2DM mouse model induced by high fat diet combined with STZ, the curative effect and mechanism are verified by combining OGTT, IPITT, serum biochemical detection, HE / PAS tissue staining, q-PCR and other technologies, and finally the purposes of improving glycometabolism of the T2DM mouse, repairing liver / kidney / pancreas target organ injuries, protecting pancreas islet beta cell functions, reducing pancreas islet beta cell functions, reducing pancreas islet beta cell functions, reducing pancreas islet beta cell functions, reducing pancreas islet beta cell functions and the like are achieved. And a theoretical basis is provided for accurate intervention of T2DM by activating an IGF-1R / PI3K-AKT signal channel and regulating and controlling the expression of a glycometabolism-related gene.

The present invention relates to macrophages engineered to express matrix metallopeptidase, and therapeutic uses thereof. Such macrophages may be used in the treatment of inflammatory conditions, including fibrotic components, in subjects, such as inflammatory organ damage. The inflammatory conditions may be acute or chronic and may involve fibrotic components.

The invention discloses screening of transcriptome biomarkers responsive to tanshinone IIA and application of the transcriptome biomarkers in sepsis diagnosis and treatment kits. Tanshinone IIA (TSA) inhibits an SAMSN1 gene in a targeted manner by up-regulating expression of mmu-miR-1896, so that an NRF2 / KEAP1 antioxidant pathway is activated, and GSDMD-mediated pyroptosis is inhibited, thereby reducing multi-organ injury. 10-20 mg / kg of TSA can significantly improve the alveolar structure of the LPS model and reduce inflammatory factors. A kit which is developed on the basis of the mechanism and is used for detecting the expression levels of mmu-miR-1896, SAMSN1 and NRF2-Kea1-GSDMD can be used for evaluating the curative effect.

Provided herein are formulations for use in enteral tube feeding, that contain one or more whole food-based ingredients, mineral nutrients, small molecules, and / or large molecules, the combination of which is effective to bind bile acids when administered to a subject in need thereof. The combination of crafted ingredients for tube feeding reduce symptoms of gastroesophageal reflux, vomiting, abdominal pain, irregular stooling (diarrhea or constipation) and to reduce organ damage including pneumonia, pharyngitis, aspiration, esophagitis, gastritis and enteritis.

The invention relates to an establishment method of a high-fat induced intestinal organ injury model and an injury intervention method for the model. The establishment method comprises the following steps: step A, preparation: preparing colon cells, PBMC cells and an organ-like culture chip for later use; step B, colon barrier construction: inoculating colon cells to the surface of a barrier membrane on the lower side of a culture chip by using an organoid culture chip to obtain a colon barrier model; step C, constructing a co-culture model, and inoculating PBMC cells to the basal side of the colon barrier on the basis of the colon barrier to obtain a human colon barrier-PBMC co-culture model; d, constructing a damage model, and performing damage treatment on the co-culture model by using a treating fluid to obtain the damage model; according to the invention, the barrier function organ-like chip is used, a human intestinal cell-PBMC co-culture model is formed by utilizing human intestinal organs and PBMC cells, and OXLDL / palmitic acid can play a role in an injured intestinal organ barrier model and form a high-fat induced intestinal organ injury model through the prepared injury treating fluid.

The invention provides application of hippophae rhamnoides extract in preparation of a product for dispelling the effects of alcohol or preventing and treating visceral organ injury caused by drinking, and belongs to the technical field of biological medicine. The invention provides application of a sea-buckthorn extract or a product containing the sea-buckthorn extract in preparation of a product for dispelling the effects of alcohol or preventing and treating visceral organ injury caused by drinking. The sea-buckthorn extract comprises sea-buckthorn fruit oil, sea-buckthorn seed oil and / or sea-buckthorn fruit pulp. The organ injury comprises alcoholic fatty liver, gastric mucosa injury or duodenum mucosa injury. The hippophae rhamnoides fruit extract (hippophae rhamnoides fruit pulp, hippophae rhamnoides fruit oil and hippophae rhamnoides fruit seed oil) and the hippophae rhamnoides fruit and Chinese wolfberry fruit composite primary pulp provided by the invention have the effects of promoting the repair of gastric mucosa and duodenal mucosa and further enhancing the immunity of gastrointestinal tracts, and can effectively prevent and / or treat gastritis, alcoholic fatty liver and duodenal mucosa injury caused by drinking; the traditional Chinese medicine composition can replace traditional medicine treatment and has the advantages of small side effect, economy, practicability, suitability for long-term administration and good compliance.

The application discloses a bat moth lepidoptera cordyceps strain and application thereof in preparation of anti-neutron radiation medicines. Samsoniella hepiali The strain is the bat moth lepidoptera cordyceps strain P969 (Pleurotus spodoleucus), which is preserved in the China Center for Type Culture Collection and has a preservation number of CCTCC NO: M 20251172 and a preservation date of May 26, 2025. Mycelium extracts of the strain can protect biological tissues against neutron radiation and repair organ damage caused by neutron radiation, so that the strain has a wide development space and a good development and application prospect.