46 results about "Benzopyrene" patented technology

The invention relates to a method for reducing harmful components in cigarette smoke by using food-grade cyclodextrin metal organic framework materials, which is characterized in that two food-grade cyclodextrin metal organic framework materials CD-MOFs are added into cigarette filters for reducing the harmful components in the cigarette smoke. The unique pore structure and rich hydroxyl functional sites of the gamma-cyclodextrin-KOH and the beta-cyclodextrin-KOH are utilized to efficiently adsorb various harmful components in the mainstream smoke of the cigarette. Compared with a control group without any adsorbent and a control group with only activated carbon as the adsorbent, the CD-MOFs has efficient capturing capability on harmful gases such as carbon monoxide, nitrosonicotine, ammonia gas, benzo [a] pyrene and phenol in the cigarette smoke, and can obviously reduce the cigarette hazard index. The two materials are simple in preparation process, high in cost benefit and good in chemical stability, are food-grade porous materials and have incomparable practical application potential compared with traditional non-food-grade porous materials, and an effective solution is provided for developing novel low-harm cigarette products.

The invention relates to the technical field of organic pollution treatment, in particular to a lignin grafted graphene oxide quantum dot material, a preparation method thereof and a polycyclic aromatic hydrocarbon degradation method, and the material is prepared by grafting reaction of alkaline lignin and graphene oxide quantum dots under alkaline conditions. When the strain is applied to degradation of polycyclic aromatic hydrocarbon, the strain and a Microbacterium sp.W1-2 strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.35223 form a synergistic degradation system. The material can significantly promote thallus growth, enhance pollutant interface mass transfer and bioavailability, and realize efficient degradation in a dark environment without photocatalysis. Experiments show that under the condition that the initial concentration of benzo [a] pyrene is 5000g / L, by adding the material, the degradation rate within 6 hours can be increased to 651.4 g / h, which is increased by 2.9 times compared with that of a single strain; and the removal rate within 72 hours reaches 86.4%. The method has the advantages of being high in degradation efficiency, short in period, high in environmental adaptability, independent of illumination and the like, and is suitable for rapidly repairing the polycyclic aromatic hydrocarbon in the industrial wastewater.

This invention discloses a benzo[a]pyrene-degrading fungus, *Nigrospora osmanthi* DL-7, for use in petroleum-contaminated soil, along with its inoculum and applications. This invention domesticated, isolated, and identified a benzo[a]pyrene-degrading strain, *Nigrospora osmanthi* DL-7, from petroleum-contaminated soil in Ningbo. DL-7 can utilize benzo[a]pyrene as a carbon source, even at an initial benzo[a]pyrene concentration of 25 mg·L⁻¹. ‑1 After culturing in an inorganic salt broth for 7 days, the pure DL-7 strain achieved a degradation rate of over 85% for benzo[a]pyrene. When prepared as a bacterial agent, the degradation rate of benzo[a]pyrene could be increased to over 90%. Therefore, this strain and its bacterial agent have good application potential in the bioremediation of polycyclic aromatic hydrocarbons.

The invention discloses a method for degrading benzo (alpha) pyrene in water by using gamma rays, belongs to the technical field of sewage treatment, and aims to solve the technical problem that the degradation efficiency and the degradation degree of the benzo (alpha) pyrene in the water by using the gamma rays in the prior art need to be further improved. An iron circulation-peroxymonosulfate composite system composed of a quaternized porous aromatic material, a sulfonated silicon-based hybrid channel material and a cross-linked beta-cyclodextrin material in a synergistic manner is constructed, and the quaternized porous aromatic material realizes stable enrichment of pollutants and peroxymonosulfate through a positive electricity interface; the sulfonated silicon-based hybrid channel material provides a continuous coordination and electron transfer environment for iron species, the cross-linked beta-cyclodextrin material performs confinement and secondary inclusion on hydrophobic pollutants and intermediates, and the sulfonated silicon-based hybrid channel material, the cross-linked beta-cyclodextrin material, the hydrophobic pollutants and the intermediates form a mutually linked reaction path in structure and function, so that construction and maintenance of a reaction micro-domain and continuous organization of the reaction path are realized, and the hydrophobic pollutants and the intermediates are subjected to continuous coordination and electron transfer. Therefore, the degradation efficiency and the cycling stability of pollutants are remarkably improved.

This invention provides a method for preparing pomegranate peel charcoal, the pomegranate peel charcoal itself, and a method for quality control thereof. The preparation method includes: drying pomegranate peel to constant weight; heat-treating the dried pomegranate peel at 180°C to 230°C for 5 to 20 minutes to obtain pomegranate peel charcoal. This method has a clear process, low energy consumption, and the obtained pomegranate peel charcoal contains less than 5 μg / kg of benzo[a]pyrene, and can enrich active ingredients such as punicalin. The quality control method includes: irradiating the sample with excitation light, collecting the light radiation signal after irradiation stops, and generating an evaluation index based on the signal. The preferred evaluation index is the initial luminescence intensity Y0, which is positively correlated with the punicalin content and antioxidant activity. By substituting the light radiation signal into a pre-established correlation model, the punicalin content and antioxidant activity of the sample can be determined. The preparation method of this invention is mild and controllable, with high safety, and the quality control method is rapid and non-destructive, making it suitable for the fields of traditional Chinese medicine decoction pieces, functional food raw materials, and dietary supplements.

The application discloses a solid-phase extraction agent and a preparation method and application thereof. Raw materials of the solid-phase extraction agent include cigar stems and shavings, and the mass ratio of the cigar stems to the shavings is 1:1-2. The above raw materials are all natural plants, and in addition to being rich in vascular systems and cellulose, the structure formed by xylem and phloem arranged in bundles and a variety of capillary pore structures make the pores rich, and the structure is provided with rich polar hydroxyl and hydrophobic aromatic functional groups. After being prepared as the solid-phase extraction agent, the structure can form pi-pi bonds and hydrogen bonds with benzo[a]pyrene, has strong adsorption capacity for the benzo[a]pyrene and high adsorption stability, and due to the rich pores, has good permeability. The speed of the benzo[a]pyrene in the solution after adsorption through the extraction column is faster, the adsorption enrichment and purification efficiency of the benzo[a]pyrene in the solution after adsorption is high, and the cost is low and the structure is green and environment-friendly.

The application relates to application of Rhodococcus in degradation of benzene[a]pyrene in a water body and belongs to the technical field of microbial engineering. A Rhodococcus activation strain is subcultured with LB liquid culture medium, the cell concentration is adjusted, and the strain is inoculated into liquid culture medium containing benzene[a]pyrene at a volume ratio of 5%, the final concentration of benzene[a]pyrene in the culture medium is 0.5 mM; and the sample is cultured in a shaking table under the conditions that the temperature is 27 DEG C, the pH is 7.0, and the rotating speed is 180 rpm for 5 days. The Rhodococcus can grow and metabolize by taking benzene[a]pyrene as the only carbon source and energy source, and the benzene[a]pyrene can be efficiently degraded. Through systematic optimization of the degradation conditions, the optimal process parameters are determined, the degradation efficiency is obviously improved, and the application has the advantages of low cost, no secondary pollution, high degradation efficiency and the like. The application provides an efficient and environment-friendly technical scheme for biological remediation of benzene[a]pyrene pollution in a water body, and has good ecological benefits and wide application prospect.

Lubricants with epoxide-based linkages for Heat Assisted Magnetic Recording (HAMR) are provided. Epoxide chemistry produces one such lubricant for magnetic media according to general formula (Ia): (Formula (Ia)) where Dn is –(CF2CF2CF2O)n-, R1, R2, R3, R4, R5 and R6 independently comprise single or branched non-fluorinated or fluorinated C1-C10 alkyl, C1-C10 alkoxy, benzene, naphthalene, anthracene, phenanthrene, dibenz[a,h]anthracene, pyrene or benzopyrene, with n being from 2 to 50.

This invention relates to the field of meat processing technology, and discloses a method for controlling the formation of benzo[a]pyrene in high-temperature roasted meat products and improving their quality. The method includes removing the skin and fascia of fresh meat to obtain lean meat; mincing and mixing the lean meat with fat; adding salt to obtain a basic meat paste; adding 0.6% to 0.7% by weight of calcium ascorbate to the basic meat paste and mixing thoroughly to obtain a modified meat paste; marinating the modified meat paste in a refrigerator away from light; stuffing it into sausages using natural casings; igniting a carbon source until it completely turns into white charcoal without an open flame; roasting and turning the sausages on a grill at a vertical distance from the carbon source to obtain high-temperature roasted meat products. This invention, by inhibiting lipid oxidation through calcium ascorbate, cuts off the carbon source supply for the formation of the carcinogen benzo[a]pyrene at its source, while also moderately inhibiting the Maillard reaction process. This method effectively reduces the formation of carcinogens in meat products while significantly improving the product's color and flavor, thus enhancing its overall quality.

The application provides a benzopyrene immunoaffinity column and a preparation method and application thereof, and a filler of the benzopyrene immunoaffinity column comprises a solid carrier coupled with a benzopyrene monoclonal antibody. The benzopyrene monoclonal antibody in the benzopyrene immunoaffinity column is prepared by using a benzopyrene hapten-carrier protein conjugate as an immunogen, so that the antibody can specifically adsorb benzopyrene substances in petroleum chemicals, effectively improves the recovery rate of benzopyrene, reduces the interference of other impurities such as lipids, proteins and pigments in the petroleum chemicals, and improves the accuracy of benzopyrene detection.

The application provides a degradation method of polycyclic aromatic hydrocarbons in coal gangue and application thereof, and the degradation method comprises the following steps: mixing coal gangue, microbial strains with biological weathering, microbial strains for degrading polycyclic aromatic hydrocarbons, nutrients and water, and culturing, so that the degradation of polycyclic aromatic hydrocarbons in the coal gangue is completed; the polycyclic aromatic hydrocarbons include any one or a combination of at least two of naphthalene, acetylene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benz[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, indeno[1,2,3-c,d]pyrene, benz[a,h]anthracene and benzo[g,h,i]perylene. The degradation method of polycyclic aromatic hydrocarbons in the coal gangue provided by the application can simultaneously degrade multiple polycyclic aromatic hydrocarbons, and has good degradation effect.

The application discloses a circularly polarized luminescent material and a preparation method thereof, and a circularly polarized organic light-emitting diode. The circularly polarized luminescent material comprises a compound shown in a general formula (I): wherein R1 is or ; X is OTf, Cl, Br, I, TFSI or PF6; and R2 is any one of a methyl group, a phenyl group, a benzyl group, a methylnaphthalene group, a methylanthracene group, a methylbenzopyrene group, a methylpyrene group, a methylphenanthrene group and a methylperylene group. The circularly polarized luminescent material improves the circularly polarized luminescent performance of the material through the synergistic effect among a chiral binaphthyl structure, a pyridine ring and a substituent group thereof, and a supermolecular structure or a fractal structure formed.

The application provides a mixed bacterial population for degrading polycyclic aromatic hydrocarbons and application thereof, and the mixed bacterial population comprises Bacillus aryabhattai with a preservation number of CCTCC No:M 2024702, Brucella with a preservation number of CCTCC No:M 2024701 and Bacillus thuringiensis with a preservation number of CCTCC No:M 2024700. The mixed bacterial population of the application can degrade typical low, medium and high ring polycyclic aromatic hydrocarbons, and can realize all-round and efficient degradation of polycyclic aromatic hydrocarbons, and the degradation efficiency of benzo[a]pyrene (BaP) can reach 87%.

The application discloses a detoxification agent and a preparation method and application thereof, and relates to the technical field of detoxification agents, and particularly relates to a detoxification agent and a preparation method and application thereof. The preparation method comprises the following steps: (1) performing crushing treatment on shelled shrimp and / or shelled crab which are cleaned and dried, then performing hydrochloric acid immersion and centrifugal treatment to obtain first precipitates, and finally performing washing and drying on the first precipitates to obtain first dried matters; (2) performing calcination, grinding, ultrasonic treatment and centrifugal treatment on the first dried matters to obtain second precipitates, then performing washing and drying on the second precipitates to obtain second dried matters; (3) performing calcination, ultrasonic treatment and centrifugal treatment on the second dried matters to obtain third precipitates, then performing washing and drying on the third precipitates to obtain the detoxification agent. The detoxification agent disclosed by the application can efficiently remove harmful substances such as zearalenone and / or benzo[a]pyrene.

The application discloses a strain of bacteria DY-11 for degrading phenanthrene and benzopyrene in oil-contaminated soil and application thereof. The strain is Achromobacter anxifer DY-11, and the preservation number is GDMCC No: 66005. Experiments prove that the strain DY-11 can utilize phenanthrene and benzopyrene as carbon sources to degrade them, and the degradation rates of the phenanthrene and the benzopyrene can reach more than 65% after the strain is cultured in inorganic salt culture solution with the initial concentration of the phenanthrene or the benzopyrene being 50 mg·L ‑1 and 25 mg·L ‑1 respectively for 7 days. Therefore, the strain DY-11 is a strain capable of degrading phenanthrene and benzopyrene and having strong tolerance to the phenanthrene and the benzopyrene, has strong adaptability to polycyclic aromatic hydrocarbons, and has good application potential in biological remediation.

The application provides a method and system for removing benzene[a]pyrene in flue gas, the method comprising: using an aqueous solution of dimethyl sulfoxide as a reaction solution; continuously feeding cooled flue gas into the reaction solution under the irradiation of an ultraviolet light source; benzene[a]pyrene in the flue gas is dissolved in the reaction solution and degraded under the action of the irradiation of the ultraviolet light source, and flue gas with benzene[a]pyrene removed is obtained. The aqueous solution of dimethyl sulfoxide is used as a photochemical reaction solution, which can not only completely dissolve benzene[a]pyrene in different phases in the flue gas, but also promote the efficient degradation of benzene[a]pyrene, so that the complete removal of benzene[a]pyrene in the flue gas can be achieved in a short time. After the liquid phase photochemical reaction condition is optimized, the removal rate of benzene[a]pyrene in the flue gas can reach more than 99%. The application has the advantages of simple and reliable operation, low investment, low energy consumption, low operation and maintenance cost, and has a wide popularization and application prospect.

This invention discloses a method for preparing ultra-high softening point environmentally friendly asphalt, comprising adding medium-temperature asphalt to a reaction vessel, adding 5-12% of a reactant, and gradually heating to 240-260°C under normal or slightly negative pressure. After the asphalt melts, the stirrer is turned on to allow the reaction to proceed fully. The asphalt is then heated to 300-350°C, and air is introduced to induce a thermal polymerization-oxidation reaction, at which point the softening point and coking value are increased, and more than 50% of 3,4-benzo[a]pyrene is removed. At the end of the reaction, the temperature is increased to 350-380°C, and the vacuum degree is increased to 0.05-0.1 MPa, further increasing the softening point and coking value, with a cumulative benzo[a]pyrene removal rate of over 95%. When the asphalt quality meets the requirements, the vacuum is stopped. The invention also provides the asphalt and its applications. This invention, by adding a certain amount of reactant to medium-temperature asphalt and then gradually increasing the reaction temperature and vacuum degree, not only increases the softening point and coking value but also further reduces benzo[a]pyrene, resulting in ultra-high softening point environmentally friendly asphalt.

The invention relates to the field of edible oil preparation, and particularly discloses a preparation method of benzopyrene-removed aromatic peanut oil. The invention relates to a preparation method of benzopyrene-removed aromatic peanut oil. The preparation method comprises the following steps: (1) parching; (2) squeezing: the peanuts are divided into A parts and B parts, the A parts of the peanuts are squeezed by a squeezer A to obtain crude oil A, the current proportion of the squeezer A is 45-48%, the B parts of the peanuts are squeezed by a squeezer B to obtain crude oil B, and the current proportion of the squeezer B is 52-63%; (3) mixing: mixing the crude oil A and the crude oil B; (4) degumming and crystal growing: carrying out hydration degumming on the mixed crude oil to obtain degummed oil, adding activated carbon into the degummed oil, and growing the crystal at 18-22 DEG C for 6-8 hours to obtain crystallized oil; (5) filtering: filtering the crystallized oil to prepare the aromatic peanut oil from which benzopyrene is removed; the preparation method has the advantages that benzopyrene is removed, and meanwhile the characteristic flavor of the peanut oil is reserved.

The invention provides an electrochemical sensor for rapidly detecting polycyclic aromatic hydrocarbon in traditional Chinese medicinal materials and a preparation method and application of the electrochemical sensor. The electrochemical sensor is a three-electrode system and comprises a working electrode, a counter electrode and a reference electrode, the working electrode is a hydrotalcite-derived carbon / reduced graphene oxide and silver nanoparticle modified glassy carbon electrode prepared by coating the surface of a glassy carbon electrode with a hydrotalcite-derived carbon / reduced graphene oxide and silver nanoparticle composite material. The polycyclic aromatic hydrocarbon is benzo [a] pyrene and phenanthrene. And putting the electrochemical sensor into the prepared traditional Chinese medicinal material test solution to measure the oxidation peak current, calculating the concentration of the polycyclic aromatic hydrocarbon according to the linear relationship between the benzo [a] pyrene and phenanthrene standard solution and the oxidation peak current, and calculating the content of the polycyclic aromatic hydrocarbon in the traditional Chinese medicinal material to be measured according to the concentration. The electrochemical sensor has relatively high sensitivity, selectivity and stability on polycyclic aromatic hydrocarbon, does not need to be subjected to complex pretreatment on traditional Chinese medicinal materials to be detected, and has a wide application prospect.

The invention provides a benzophenanthrene polycyclic aromatic hydrocarbon hapten, a monoclonal antibody, a hybridoma cell strain and application, and relates to the technical field of immunodetection. According to the invention, triphenylene hapten is synthesized, triphenylene complete antigen is prepared, cell fusion and indirect competitive enzyme-linked immunosorbent assay screening are carried out, a hybridoma cell strain is obtained, the hybridoma cell strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.46732, and the hybridoma cell strain can be used for preparing a triphenylene monoclonal antibody. The monoclonal antibody secreted by the hybridoma cell strain has good detection sensitivity to benzo [a] pyrene, benzo [a] anthracene, benzo [b] anthracene, dibenzo [a, h] anthracene and benzo [k] anthracene, the IC50 values are 6.3 ng / mL, 19.4 ng / mL, 6.8 ng / mL, 55.5 ng / mL and 21.7 ng / mL respectively, and the monoclonal antibody can be used for establishing an immunological detection method for benzophenanthrene polycyclic aromatic hydrocarbons.

The utility model discloses a cold press for producing special-grade virgin camellia oil, which comprises a base, a support frame arranged at the rear end of the base, a hydraulic device I arranged at the top of the support frame, a hydraulic rod I mounted in the hydraulic device I, a pressing plate fixedly mounted at the bottom of the hydraulic rod I, and a fixing plate arranged in the middle of the front end of the support frame, a mounting hole is formed in the front end of the top of the fixing plate, an extrusion cavity is mounted in the mounting hole, a second hydraulic device is arranged at the rear end of the top of the fixing plate, a second hydraulic rod is mounted in the second hydraulic device, and a connecting plate is fixedly mounted at the bottom of the second hydraulic rod. The hydraulic device is adopted for cold pressing of tea seeds, heating is not needed, generation of benzopyrene, acrylamide and other cancerogenic substances in the pressing process is avoided, the oil yield is high, and the oil residue separation effect is good.

The utility model provides roasting furnace flue gas treatment equipment, and relates to the technical field of flue gas treatment. The system comprises an SNCR (selective non-catalytic reduction) denitration device, a metallurgical coke adsorption device, an RTO (regenerative thermal oxidizer) heat storage high-temperature combustion device, a wet desulfurization device, a wet electrostatic precipitation device and an induced draft fan. The SNCR denitration device can remove nitric oxide in flue gas through dry denitration, the metallurgical coke adsorption device is used for removing part of asphalt smoke and part of benzopyrene in the flue gas, and the RTO heat storage high-temperature combustion device is used for removing residual asphalt smoke, benzopyrene and VOCS in the flue gas. And finally, sulfur dioxide and dust in the flue gas are removed through a wet desulphurization device and a wet electric dust removal device. The roasting furnace flue gas treatment equipment provided by the utility model not only can treat sulfur dioxide, dust and asphalt fume in the flue gas, but also can reduce the content of VOCS and benzopyrene, so that the flue gas treatment effect is effectively improved; and ultralow emission of flue gas is realized.

The invention discloses a benzo [a] pyrene on-site rapid quantitative detection method and system and a storage medium. The detection method comprises the following steps: automatically collecting an original sample fluorescence spectrum of a to-be-detected sample, adding a preset amount of a standard solution of a safety standard substance, collecting a labeled spectrum, establishing a dual-mode dynamic correction framework, and realizing self-calibration by utilizing the response of the labeled safety standard substance; the signal intensity of a safety standard substance peak and a benzo [a] pyrene peak is extracted according to a preset peak window, the response coefficient and the concentration increment of a standard substance are calculated through an internal standard method, and the concentration of benzo [a] pyrene in a sample is calculated through a relative response factor. Complex correction and quantification processes are carried in a storage medium or a detection system and converted into one-button operation, and the method can be used for a small fluorescence spectrometer to realize on-site rapid quantitative detection of benzo [a] pyrene.

An artificial nanocarrier with antioxidant function, its preparation method, and its application, involving microbiology. It utilizes genetically engineered *Escherichia coli*. Escherichia coli :: ompA‑st Based on the cell membrane, it loads components via the SpyTag / SpyCatcher protein covalent linking system. Escherichia coli :: gshB‑sc Glutathione synthase B tagged with SpyCatcher was expressed. Two engineered bacterial strains, with preservation numbers GDMCC No. 67612 and 67613, were constructed, expressing the OmpA-st membrane protein and the GshB-sc fusion protein, respectively. Antioxidant artificial nanocarriers were obtained through disruption, purification, membrane fragment separation, covalent coupling, and ultracentrifugation. These carriers effectively scavenge reactive oxygen species, reduce ROS levels in recipient bacteria under benzo[a]pyrene stress, enhance cellular antioxidant capacity, and help cells resist oxidative stress.

The invention belongs to the technical field of edible vegetable oil processing, and particularly relates to a grease processing method. Specifically, the invention provides a method for reducing benzopyrene in grease, the method comprises the following steps: filtering the grease by using an activated carbon layer and other optional filter media, and the activated carbon layer is contacted with the grease before the other filter media.

The invention belongs to the technical field of detection methods, and particularly relates to a method for detecting the content of benzo (a) pyrene in traditional Chinese medicine by GC-MSMS (Gas Chromatography-Mass Spectrometry-Mass Spectrometry) and application of the method. The invention discloses a method for detecting the content of benzo (a) pyrene in traditional Chinese medicine by GC-MSMS (Gas Chromatography-Mass Spectrometer). The method comprises the following steps: preparing a standard solution to obtain a standard curve; pretreating the traditional Chinese medicine sample to obtain a solid sample; extracting the solid sample to obtain a sample to be detected; the sample to be detected is subjected to GC-MSMS detection, an external standard method is adopted, and the content of benzo (a) pyrene in the traditional Chinese medicine is obtained through quantification of the standard curve. According to the method for detecting the benzo (a) pyrene content in the traditional Chinese medicine, GC-MSMS is matched with an external standard method for quantitative determination, compared with a traditional internal standard method for quantitative determination, the detection cost is reduced, tedious detection steps are optimized, and in cooperation with traditional Chinese medicine sample pretreatment, it is ensured that the purification effect is good, the matrix effect is low, and the detection accuracy is high. The method provided by the invention is rapid, sensitive and high in accuracy.

The invention provides a method for reducing harmful components in cigarette smoke by using a metal organic framework material, and belongs to the technical field of metal organic frameworks. The MOFs material is respectively HKUST-1, MOF-74 (Zn), NH2-MIL-53 (Al) or NO2 (MIL-53) (Al), and the MOFs material is a metal organic framework material. The obtained material is diversified in structure, adjustable in pore diameter and good in chemical stability, and the materials can efficiently and synergistically adsorb various harmful ingredients in main stream smoke of cigarettes through unique pore structures, high specific surface area and specific functional groups. The MOFs material is added into a traditional cellulose acetate filter tip, and compared with a control group which is not added with any adsorbent and is added with activated carbon as the adsorbent, MOFs have efficient capturing capacity on harmful gases such as carbon monoxide, nitrosonicotine, ammonia gas, benzopyrene and phenol in cigarette smoke; the cigarette perniciousness index can be obviously reduced, and the smoking quality is improved. The preparation process is simple, the cost benefit is high, and an effective solution is provided for developing novel low-harm cigarette products.

This invention relates to the technical field of analytical chemistry, specifically to an analytical detection method for benzo[a]pyrene in nicotine bags, comprising the following steps: placing the nicotine bag sample in a container, adding an internal standard and a cyclohexane solution to the container to obtain a mixture, and performing shaking extraction, centrifugation, and filtration, taking the supernatant as the test solution; wherein the internal standard includes benzo[a]pyrene-D12; detecting the test solution using gas chromatography-tandem mass spectrometry, and performing quantitative analysis of benzo[a]pyrene based on the response signal of the internal standard. This invention, through the selection of a specific internal standard and cyclohexane pretreatment, can be specifically used for the quantitative analysis of benzo[a]pyrene in nicotine bags, and has the advantages of efficient and simple pretreatment and effective overcoming interference from complex matrices.

The application discloses a composite bacterium for degrading and repairing benzo[a]pyrene as well as a functional bacterium agent and application thereof. The composite bacterium comprises Mycolicibacterium sp. 142 and Stenotrophomonas sp. 143, and the preservation numbers are CCTCC NO: M 20221294 and CCTCC NO: M 20221295 respectively. The composite bacterium obtained by separation and screening can effectively degrade benzo[a]pyrene and various polycyclic aromatic hydrocarbons; can quickly and effectively degrade more than 90% of benzo[a]pyrene in an inorganic salt culture medium; meanwhile, the composite bacterium agent can degrade more than 80% of high-concentration benzo[a]pyrene in soil in a short time, significantly improves the degradation effect of a single strain, and effectively solves the problem of hazards of residual benzo[a]pyrene to soil or water environment, crops and human health.

The present disclosure discloses a method for reducing benzopyrene and plasticizers in capsicum oleoresin and a product obtained thereby. The method includes steps: dissolving crude oil paste of capsicum oleoresin as raw material with a small molecular alcohol solvent to obtain a small molecular alcohol solution of capsicum oleoresin; performing extraction and back-extraction with CO2 and small molecular alcohol solvents, respectively in a supercritical two-stage series mode; performing extracting on the small molecular alcohol solution by using CO2 in extraction kettle I, to obtain a CO2 extract phase and a raffinate phase (small molecular alcohol phase); obtaining high-purity capsicum oleoresin with reduced benzopyrene and plasticizers by conducting vacuum concentration and residual solvent removal on the small molecular alcohol phase; performing back-extraction on the CO2 extract phase by using a small molecular alcohol solvent after the CO2 extract phase enters extraction kettle II, so as to obtain small molecular alcohol phase, which is used for dissolving the crude oil paste of capsicum oleoresin in the next batch. The present disclosure improves the content and yield of refined capsicum oleoresin products and removing odor, benzopyrene, and plasticizers. Additionally, in the present disclosure, the removal efficiency of plasticizers, benzopyrene, and odors in capsicum oleoresin extracts may be improved, and the levels of benzopyrene, plasticizers, and inherent odors in the capsicum extract are reduced to extremely low levels, so that capsicum oleoresin may be significantly superior to products produced in prior art.