142 results about "Benzopyrene" patented technology

The invention discloses a preparation method and application of a magnetic biochar-loaded bacterium composite functional material, magnetic biochar (MBC) is used as a carrier to load and fix Achromobacter denitrificans BP1 (Achromobacter denitrificans BP1, the NCBI login number is CP154792), and a magnetic biochar-loaded functional bacterium (MBC-coated BP1) is obtained. The invention relates to a water and soil purifying agent which is used for removing pollutants in water and / or soil, the pollutants comprise polycyclic aromatic hydrocarbons (PAHs) and / or heavy metals (HM) and the like, benzo [a] pyrene (BaP) and phenanthrene (Phe) in the soil can be efficiently degraded, Cr (VI) is removed, nitrate (NO3 <->) is metabolized, the soil nutrient level (organic matters, nitrogen, phosphorus and the like) can be increased, the diversity and structural functions of soil microbial communities are improved, and the water and soil purifying agent can be used for purifying water and / or soil. And a green and efficient repairing material and a technical support are provided for a compound pollution complex site.

The invention provides a method for degrading benzo [a] pyrene (BaP) and application of the benzo [a] pyrene (BaP). According to the degradation method, a strain of bacillus thuringiensis PAHc-a with the preservation number of CCTCC No: M 2024700 is used for degrading BaP, and the natural degradation rates of the bacillus thuringiensis PAHc-a respectively reach 17.80%, 63.94% and 83.92% in 3 days, 6 days and 9 days. In order to further improve the degradation rate of benzopyrene, another two degradation bacteria, namely bacillus aryabhattai PAHA-e with the preservation number of CCTCC No: M 2024702 and brucella PAHB-d with the preservation number of CCTCC No: M 2024701, are simultaneously screened in the invention; the three strains are used as a composite flora, cultured and inoculated into pollutants, and the 9-day natural degradation rate of benzopyrene can be increased to 87.10%.

The invention discloses a Pseudomonas nicosulfurones bacterial strain and an application of the Pseudomonas nicosulfurones bacterial strain in degradation of phenanthrene and benzopyrene. According to the invention, a degradation strain Pseudomonas nicosulfurensis DY-8, which takes phenanthrene and benzopyrene as carbon sources, is obtained by domestication, separation and identification from petroleum-polluted soil in Ningbo. The invention also relates to a method for preparing the degradation strain Pseudomonas nicosulfurensis DY-8 with phenanthrene and benzopyrene as carbon sources. The DY-8 can utilize phenanthrene and benzopyrene as carbon sources, and after the DY-8 is cultured in an inorganic salt culture solution with the initial concentration of phenanthrene and benzopyrene being 50 mg.L <-1 > and 25 mg.L <-1 > respectively for 7 days, the degradation rates of phenanthrene and benzopyrene can reach 70% or above. Therefore, the strain has good application potential in the aspect of bioremediation of polycyclic aromatic hydrocarbon.

The invention discloses a plant lactobacillus DACNJS22 strain and application thereof in preventing / relieving / treating biological metabolic toxicity of benzopyrene. The lactobacillus plantarum DACNJS22 is preserved in the China Center for Type Culture Collection, and the preservation number of the lactobacillus plantarum DACNJS22 is CCTCC (China Center for Type Culture Collection) NO: M20241957. The plant lactobacillus DACNJS22 disclosed by the invention can be used for efficiently reducing in-vivo bioconversion of benzopyrene into a key carcinogen (benzo (a) pyrene-7, 8-dihydro-diol-9, 10-epoxide) by inhibiting the expression level of cytochrome P450 family genes, and can be used for reducing the biological metabolic toxicity of benzopyrene from the source, so that the application of the plant lactobacillus DACNJS22 in the field of bioconversion of benzopyrene into the key carcinogen is realized. The intake of benzopyrene-polluted food and the damage of benzopyrene exposed to the environment to animal bodies or human bodies can be reduced; the compound can effectively prevent and / or relieve and / or treat tissue and organ injuries caused by oral exposure of benzopyrene and related cognitive diseases.

The invention relates to the technical field of edible oil processing, and discloses high-quality special sesame oil and a preparation method thereof.The method comprises the following steps that 1, under the condition that the oxygen concentration is lower than 0.5 v%, an oil material and first edible oil are mixed and then subjected to first-stage heating and second-stage heating in sequence, and a first solid-liquid mixture is obtained; (2) carrying out first solid-liquid separation on the first solid-liquid mixture to obtain first clear oil and first oil residues; wherein the first-stage heating is carried out under negative pressure, and the second-stage heating is carried out under normal pressure; the temperature of the first-stage heating is 30-160 DEG C lower than that of the second-stage heating. The vegetable oil prepared by the method is high in nutrient substance and flavor substance content, low in benzopyrene content, low in acid value and peroxide value, less in oil smoke during cooking, short in processing time and high in aroma production efficiency and nutrient substance extraction rate; in addition, the plant oil prepared by the method does not need to be hydrated and degummed, and the phosphorus content in the plant oil is low.

The invention belongs to the technical field of analysis and detection, and discloses a method for detecting four polycyclic aromatic hydrocarbons in vegetable oil through gas chromatography-tandem mass spectrometry. According to the method, a saponification and degreasing method and dispersive liquid-liquid microextraction (DLLME) are organically combined, so that sample pretreatment is effectively simplified, and the advantages of high selectivity and stable isotope internal standard quantification of gas chromatography-tandem mass spectrometry (GC-MS / MS) are combined; a novel method for detecting four important polycyclic aromatic hydrocarbons (PAH4) including benzo (a) pyrene (BAP), benzo (a) anthracene (BAA), benzo (b) fluoranthene (BBF) and chrysene (CHR) in common edible vegetable oil is established. According to the method, the sample pretreatment is simple and rapid, the personnel environment is relatively friendly, and the detection limit and the accuracy can meet the requirements of monitoring PAH4 in common edible vegetable oil.

The invention discloses a bacterium DY-11 for degrading phenanthrene and benzopyrene in petroleum-contaminated soil and application of the bacterium DY-11. The bacterial strain is Achromobacter expander DY-11, and the preservation number of the bacterial strain is GDMCC No: 66005. Experiments prove that the strain DY-11 can respectively utilize phenanthrene and benzopyrene as carbon sources to degrade phenanthrene and benzopyrene, and after the strain DY-11 is cultured in inorganic salt culture solutions with the initial concentration of phenanthrene and benzopyrene being 50 mg.L <-1 > and 25 mg.L <-1 > respectively for 7 days, the degradation rates of phenanthrene and benzopyrene can reach 65% or above. Therefore, the DY-11 is a strain which can degrade phenanthrene and benzopyrene and has very strong tolerance to phenanthrene and benzopyrene, has strong adaptability to polycyclic aromatic hydrocarbons, and has better application potential in the aspect of bioremediation.

The invention belongs to the technical field of biological agents and bioremediation, and particularly relates to a PAHs degrading strain as well as a fungicide and application thereof. The invention provides a strain for degrading PAHs (polycyclic aromatic hydrocarbons), the strain is Brucella anthrapi M1, and the preservation number of the strain is CCTCC (China Center for Type Culture Collection) M 2024922. The strain M1 can grow and breed by taking benzo (a) pyrene (BaP), pyrene or phenol as the unique carbon source and energy, and can quickly degrade PAHs; the strain is cultured in an inorganic salt culture medium with BaP, pyrene and phenol initial concentration of 5 mg / L for 5 days, and the degradation efficiency reaches 81%, 37% and 28% respectively. The bacterial strain is prepared into a microbial inoculum through crosslinking of sodium alginate and calcium chloride, and the degradation efficiencies of the microbial inoculum to BaP, pyrene or phenol respectively reach 46%, 37% and 80%. After the bacterial strain is prepared into a bacterial agent, the BaP degradation efficiency of the bacterial agent can reach 80%. Therefore, the strain and the fungicide thereof have a good application prospect in the bioremediation of PAHs (especially BaP) polluted soil or water.

The invention belongs to the technical field of biology, and particularly relates to a hybridoma cell strain BAP1C12, an anti-benzo [a] pyrene monoclonal antibody generated by the hybridoma cell strain BAP1C12 and application. The hybridoma cell strain BAP1C12 provided by the invention can be used for preparing a high-sensitivity anti-benzo [a] pyrene monoclonal antibody, and the titer measured by an enzyme-linked immunosorbent assay (ELISA) method can reach 2 * 10 < 5 >. The anti-benzo [a] pyrene monoclonal antibody provided by the invention is high in sensitivity and good in specificity, and the 50% inhibition concentration IC50 of the anti-benzo [a] pyrene monoclonal antibody to benzo [a] pyrene is 0.45 ng / mL. The anti-benzo [a] pyrene monoclonal antibody provided by the invention is high in sensitivity and good in specificity, the cross reactions of the anti-benzo [a] pyrene monoclonal antibody to the structural analogues of fluoranthene, benzo [b] fluoranthene, indeno [1, 2, 3-cd] pyrene, benzo [a] anthracene, benzo [k] fluoranthene, anthracene, phenanthrene, acenaphthene, fluorene and naphthalene are less than 3.5%, and the anti-benzo [a] pyrene monoclonal antibody can be applied to benzo [a] pyrene content determination.

The invention belongs to the technical field of food processing, and discloses a preparation method of fragrant nutritional peanut oil, which is based on a second-order squeezing process, and specifically realizes preparation of high-nutrient fragrant peanut oil through the working procedures of raw material soaking, irradiation germination, drying, crushing, pre-squeezing, puffing, solid state fermentation, steaming and frying, squeezing and the like. The obtained peanut oil has the characteristics of high extraction yield, rich nutrient substances, strong flavor, low content of harmful substances such as aflatoxin and benzopyrene, and the like.

The invention relates to a method for reducing harmful components in cigarette smoke by using food-grade cyclodextrin metal organic framework materials, which is characterized in that two food-grade cyclodextrin metal organic framework materials CD-MOFs are added into cigarette filters for reducing the harmful components in the cigarette smoke. The unique pore structure and rich hydroxyl functional sites of the gamma-cyclodextrin-KOH and the beta-cyclodextrin-KOH are utilized to efficiently adsorb various harmful components in the mainstream smoke of the cigarette. Compared with a control group without any adsorbent and a control group with only activated carbon as the adsorbent, the CD-MOFs has efficient capturing capability on harmful gases such as carbon monoxide, nitrosonicotine, ammonia gas, benzo [a] pyrene and phenol in the cigarette smoke, and can obviously reduce the cigarette hazard index. The two materials are simple in preparation process, high in cost benefit and good in chemical stability, are food-grade porous materials and have incomparable practical application potential compared with traditional non-food-grade porous materials, and an effective solution is provided for developing novel low-harm cigarette products.

The invention provides a 6, 6-dimethyl-6H-benzo [cd] pyrene compound, an intermediate thereof, an organic electroluminescent device and a display device, and relates to the technical field of organic photoelectricity. The structural general formula of the 6, 6-dimethyl-6H-benzo [cd] pyrene compound is # imgabs0 #, and m and n are respectively and independently selected from 0 or 1; hydrogen atoms in the formula I are respectively and independently substituted or not substituted by deuterium atoms, F, CN, C1-C12 alkyl, C6-C30 aryl or C3-C30 aromatic heterocyclic groups. According to the present invention, the structure of the 6, 6-dimethyl-6H-benzo [cd] pyrene compound is designed, such that the obtained 6, 6-dimethyl-6H-benzo [cd] pyrene compound has excellent performance, and the organic light-emitting device prepared by using the 6, 6-dimethyl-6H-benzo [cd] pyrene compound as the light-emitting layer material has characteristics of low driving voltage, high current efficiency and long service life.

The invention belongs to the field of bioengineering, and particularly relates to an engineering bacterium for expressing transporter TBDT11 by an outer membrane as well as preparation and application of the engineering bacterium. The preparation method of the engineering bacteria comprises the following steps: (1) connecting a TBDT11 gene with an escherichia coli expression vector pET-28a to obtain a recombinant vector pET-TBDT11; (2) transforming the recombinant vector pET-TBDT11 into an escherichia coli competent cell, so as to obtain an engineering bacterium EcN-pET-TBDT11; and (3) carrying out induced expression on the engineering bacterium EcN-pET-TBDT11 by using isopropyl thiogalactoside IPTG, and culturing to obtain the engineering bacterium of the transporter TBDT11 expressed by the outer membrane of the bacterium. The engineering bacteria prepared by the method can be ingested to reduce the benzopyrene content in the intestinal environment, and the preparation method has the advantages of simplicity, high efficiency, safety, no toxicity, no need of protein purification, low cost and the like.

The invention relates to the technical field of organic pollution treatment, in particular to a lignin grafted graphene oxide quantum dot material, a preparation method thereof and a polycyclic aromatic hydrocarbon degradation method, and the material is prepared by grafting reaction of alkaline lignin and graphene oxide quantum dots under alkaline conditions. When the strain is applied to degradation of polycyclic aromatic hydrocarbon, the strain and a Microbacterium sp.W1-2 strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.35223 form a synergistic degradation system. The material can significantly promote thallus growth, enhance pollutant interface mass transfer and bioavailability, and realize efficient degradation in a dark environment without photocatalysis. Experiments show that under the condition that the initial concentration of benzo [a] pyrene is 5000g / L, by adding the material, the degradation rate within 6 hours can be increased to 651.4 g / h, which is increased by 2.9 times compared with that of a single strain; and the removal rate within 72 hours reaches 86.4%. The method has the advantages of being high in degradation efficiency, short in period, high in environmental adaptability, independent of illumination and the like, and is suitable for rapidly repairing the polycyclic aromatic hydrocarbon in the industrial wastewater.

The invention discloses a coking enterprise peripheral soil benzopyrene monitoring distribution optimization method based on a CALPUFF model, and belongs to the technical field of environmental monitoring, and the method comprises the steps: obtaining annual discharge amount and pollution source parameters of benzopyrene, inputting the CALPUFF model, and integrating meteorological, terrain and land coverage data to construct a three-dimensional meteorological element field, the atmospheric diffusion and dry-wet sedimentation process of benzopyrene is simulated to determine the pollution range; monitoring points are arranged in high-value areas around an enterprise and a factory boundary based on settlement flux distribution, the downstream of a prevailing wind direction and a soil background value area, and the benzopyrene content in soil is detected by referring to the national standard. The monitoring efficiency and the data reliability are improved by accurately simulating the pollution transmission process and the differentiated point distribution strategy, and the method is suitable for monitoring the benzopyrene pollution of the soil around the coking enterprise.

The invention discloses a refining process capable of reducing loss of squalene in camellia oleifera seed oil. The refining process comprises the following steps: 1) degumming crude camellia oleifera seed oil; (2) deacidification; 3) deodorization: introducing water vapor into the deacidified oil subjected to deacidification treatment under the condition that the temperature is 110-120 DEG C, carrying out distillation deodorization for 30-45 minutes, then cooling, adding the nigre obtained by deacidification in the step 2), and carrying out adsorption treatment; 4) decoloring; and 5) winterizing to obtain the refined camellia oleosa seed oil. According to the method, the temperature of steam distillation deodorization can be reduced, the time of steam distillation deodorization is shortened, the energy consumption of steam distillation deodorization and the loss of squalene are reduced, and the quality of the refined camellia oleosa seed oil is improved; moreover, the removal rate of benzo (a) pyrene, di-n-butyl phthalate (DBP) and di (2-ethyl) hexyl phthalate (DEHP) can be effectively improved, and the safety of the camellia oleifera seed oil is improved.

The invention discloses a Schiff base functionalized silica aerogel / benzo (a) pyrene molecularly imprinted material and application thereof, pyrene is used as a virtual template molecule, and Schiff base functionalized silica aerogel is added; adding a functional monomer, a cross-linking agent and an initiator; and preparing the Schiff base functionalized silicon dioxide aerogel / benzo (a) pyrene molecularly imprinted material by adopting a surface imprinting polymerization method. The silicon dioxide aerogel is used as a base material, and the adsorption efficiency of benzo (a) pyrene is enhanced through the porous structure and high porosity of the silicon dioxide aerogel. Schiff base groups are introduced to form strong interaction with benzo (a) pyrene molecules, so that the adsorption capacity is further improved. And by combining a molecular imprinting technology, holes highly matched with benzo (a) pyrene are formed on the surface of the Schiff base functionalized aerogel, so that the material is endowed with high selectivity. Pyrene is used as a virtual template, so that the use of benzo (a) pyrene is avoided, and the environmental risk and the material cost are reduced.

The invention provides a method for reducing benzopyrene and a plasticizer in capsicum oleoresin and a product thereof. The method specifically comprises the following steps: by taking crude extract ointment of capsicum oleoresin as a raw material, dissolving the crude extract ointment in a small-molecular alcohol solvent, and respectively performing extraction and reverse extraction by CO2 and the small-molecular alcohol solvent in a supercritical two-stage series mode; in an extraction kettle I, CO2 is adopted for extracting the small molecule alcohol solution, and a CO2 extraction phase and a small molecule alcohol phase are obtained; carrying out vacuum concentration and solvent residue removal treatment on the small molecular alcohol phase to obtain capsicum oleoresin with high purity and low benzopyrene and plasticizer; the CO2 extraction phase enters an extraction kettle II and is subjected to reverse extraction with a small-molecule alcohol solvent, and the obtained small-molecule alcohol phase is used for dissolving the capsicum oleoresin crude extraction ointment of the next batch. According to the invention, the removal effect of the plasticizer, benzopyrene and odor in the chili oil extract is effectively improved, so that the benzopyrene, the plasticizer and inherent flavor and odor in the chili oil extract reach extremely low levels, which are obviously superior to those of commercially available products.

The present invention discloses a Chinese yam meat ball steaming production method which comprises the following steps: fresh Chinese yams are washed and peeled, and Chinese yam powder is prepared; cleaning and peeling fresh pork to prepare minced pork; mixing and stirring the crushed Chinese yams, the minced pork, edible salt, bruised ginger, bruised scallions, pepper powder, paprika powder, peanut oil, sesame oil, sweet potato starch and egg white to form a mixed material; and preparing the mixed material into spherical balls, putting the spherical balls into a steamer, and steaming and curing to obtain the Chinese yam meat balls. The Chinese yam meatballs are prepared by steaming and curing, and the Chinese yam meatballs are prepared by the processing method, so that the problem that cancerogenic substances benzopyrene and acrylamide are easily generated in the process of preparing the Chinese yam meatballs by high-temperature frying and are not beneficial to human health is solved, and the Chinese yam meatballs are more in line with the life concept of healthy diet pursued by modern people.

The invention discloses a Bosea thioxidans DY-5 strain and an application of the Bosea thioxidans DY-5 strain in degradation of phenanthrene and benzopyrene. The strain Bosea thioxidans DY-5 is obtained through domestication, separation and identification from petroleum-contaminated soil of Ningbo, phenanthrene and benzopyrene can be used as carbon sources, and after the strain is cultured in an inorganic salt culture solution with the initial concentration of phenanthrene and benzopyrene being 50 mg.L <-1 > and 25 mg.L <-1 > respectively for 7 days, the degradation rate of phenanthrene and benzopyrene can reach 75% or above. Therefore, the strain has good application potential in the aspect of bioremediation of polycyclic aromatic hydrocarbon.

Provided is a laccase derived from a strain of Versicolor versicolor. The present invention relates to a polypeptide comprising the mature polypeptide of the amino acid sequence of SEQ ID NO: 2. Compared to existing laccases, the polypeptide of the present invention exhibits a higher benzo[a]pyrene removal effect.

This invention discloses a benzo[a]pyrene-degrading fungus, *Nigrospora osmanthi* DL-7, for use in petroleum-contaminated soil, along with its inoculum and applications. This invention domesticated, isolated, and identified a benzo[a]pyrene-degrading strain, *Nigrospora osmanthi* DL-7, from petroleum-contaminated soil in Ningbo. DL-7 can utilize benzo[a]pyrene as a carbon source, even at an initial benzo[a]pyrene concentration of 25 mg·L⁻¹. ‑1 After culturing in an inorganic salt broth for 7 days, the pure DL-7 strain achieved a degradation rate of over 85% for benzo[a]pyrene. When prepared as a bacterial agent, the degradation rate of benzo[a]pyrene could be increased to over 90%. Therefore, this strain and its bacterial agent have good application potential in the bioremediation of polycyclic aromatic hydrocarbons.

The invention discloses a method for degrading benzo (alpha) pyrene in water by using gamma rays, belongs to the technical field of sewage treatment, and aims to solve the technical problem that the degradation efficiency and the degradation degree of the benzo (alpha) pyrene in the water by using the gamma rays in the prior art need to be further improved. An iron circulation-peroxymonosulfate composite system composed of a quaternized porous aromatic material, a sulfonated silicon-based hybrid channel material and a cross-linked beta-cyclodextrin material in a synergistic manner is constructed, and the quaternized porous aromatic material realizes stable enrichment of pollutants and peroxymonosulfate through a positive electricity interface; the sulfonated silicon-based hybrid channel material provides a continuous coordination and electron transfer environment for iron species, the cross-linked beta-cyclodextrin material performs confinement and secondary inclusion on hydrophobic pollutants and intermediates, and the sulfonated silicon-based hybrid channel material, the cross-linked beta-cyclodextrin material, the hydrophobic pollutants and the intermediates form a mutually linked reaction path in structure and function, so that construction and maintenance of a reaction micro-domain and continuous organization of the reaction path are realized, and the hydrophobic pollutants and the intermediates are subjected to continuous coordination and electron transfer. Therefore, the degradation efficiency and the cycling stability of pollutants are remarkably improved.

This invention provides a method for preparing pomegranate peel charcoal, the pomegranate peel charcoal itself, and a method for quality control thereof. The preparation method includes: drying pomegranate peel to constant weight; heat-treating the dried pomegranate peel at 180°C to 230°C for 5 to 20 minutes to obtain pomegranate peel charcoal. This method has a clear process, low energy consumption, and the obtained pomegranate peel charcoal contains less than 5 μg / kg of benzo[a]pyrene, and can enrich active ingredients such as punicalin. The quality control method includes: irradiating the sample with excitation light, collecting the light radiation signal after irradiation stops, and generating an evaluation index based on the signal. The preferred evaluation index is the initial luminescence intensity Y0, which is positively correlated with the punicalin content and antioxidant activity. By substituting the light radiation signal into a pre-established correlation model, the punicalin content and antioxidant activity of the sample can be determined. The preparation method of this invention is mild and controllable, with high safety, and the quality control method is rapid and non-destructive, making it suitable for the fields of traditional Chinese medicine decoction pieces, functional food raw materials, and dietary supplements.

The application discloses a solid-phase extraction agent and a preparation method and application thereof. Raw materials of the solid-phase extraction agent include cigar stems and shavings, and the mass ratio of the cigar stems to the shavings is 1:1-2. The above raw materials are all natural plants, and in addition to being rich in vascular systems and cellulose, the structure formed by xylem and phloem arranged in bundles and a variety of capillary pore structures make the pores rich, and the structure is provided with rich polar hydroxyl and hydrophobic aromatic functional groups. After being prepared as the solid-phase extraction agent, the structure can form pi-pi bonds and hydrogen bonds with benzo[a]pyrene, has strong adsorption capacity for the benzo[a]pyrene and high adsorption stability, and due to the rich pores, has good permeability. The speed of the benzo[a]pyrene in the solution after adsorption through the extraction column is faster, the adsorption enrichment and purification efficiency of the benzo[a]pyrene in the solution after adsorption is high, and the cost is low and the structure is green and environment-friendly.

The application discloses a benzo[a]pyrene-degrading fungus Arthrinium acutiapicum DL-5 in petroleum contaminated soil, a microbial inoculum of the Arthrinium acutiapicum DL-5 and application of the Arthrinium acutiapicum DL-5. ‑1 The benzo[a]pyrene-degrading rate of the pure Arthrinium acutiapicum DL-5 to benzo[a]pyrene can reach more than 75% after the Arthrinium acutiapicum DL-5 is cultured in an inorganic salt culture solution with an initial concentration of 25 mg / L of benzo[a]pyrene for 7 days. After the Arthrinium acutiapicum DL-5 is made into a microbial inoculum, the benzo[a]pyrene-degrading rate of the Arthrinium acutiapicum DL-5 to benzo[a]pyrene can be promoted to more than 80%, and therefore, the Arthrinium acutiapicum DL-5 and the microbial inoculum have good application potential in the bioremediation of polycyclic aromatic hydrocarbons.

The present research aims to successfully establish an acute gout mouse model, and to observe the joint and organ pathological changes of the animal and detect the joint and blood related indexes to evaluate the stability of the model, so as to make an acute gout mouse model with good stability, good repeatability, practicability and closeness to clinic, facilitate further research on the pathogenesis and pathophysiological changes of acute gout, and provide a certain basis for how to implement the protective intervention measures of acute gout. The present application induces an acute gout mouse model with good stability and repeatability, practicability and closeness to clinic through injecting aromatic oil and benzopyrene into the mouse, electric shock stimulation, lithium hydroxide stimulation, hydroxyacetic acid gavage and blue light irradiation, and the acute gout model induced by multiple factors can better simulate the characteristics of human acute gout.

The invention discloses an on-site rapid detection method and equipment for benzopyrene in soil, and the method comprises the following steps: weighing a target soil sample, adding a solvent into the target soil sample, and carrying out ultrasonic extraction on a mixture of the target soil sample and the solvent to respectively obtain first supernate; adding the first supernate into a display reagent to carry out color development reaction, so as to obtain a polymer with a first color; and carrying out color comparison on the polymer with the first color and a color developing card, and obtaining the pollution grade of benzopyrene in the target soil sample according to the concentration grade corresponding to the color of the color developing card. By means of the method, the whole process operation from soil sampling to pollution grading can be completed within 60 minutes, the detection sensitivity needs to reach the order of magnitude of 0.5 mg / kg, and the matrix interference resistance needs to meet the requirement for common soil types with the organic matter content smaller than 15%.

This invention discloses an enzyme-linked immunosorbent assay (ELISA) kit for detecting benzo[a]pyrene in edible oil, kitchen fumes, and vehicle exhaust. The kit includes: a sample reconstitution solution, an enzyme conjugate / antibody diluent, a standard / sample diluent, a benzo[a]pyrene standard, a benzo[a]pyrene-specific mouse monoclonal antibody, an enzyme conjugate, an ELISA plate coated with benzo[a]pyrene antigen, a chromogenic solution, a stop solution, and a washing solution. This invention also discloses a method for detecting benzo[a]pyrene in edible oil, kitchen fumes, and vehicle exhaust using the kit, including sample pretreatment, ELISA detection, and result processing and analysis. The sample reconstitution solution, enzyme conjugate / antibody diluent, and standard / sample diluent provided by this invention overcome the solubility problem of benzo[a]pyrene in edible oil, kitchen fumes, and vehicle exhaust while maintaining antibody sensitivity, and can also significantly improve antibody titer, thus possessing significant value for widespread application.

The invention belongs to the technical field of resource reutilization, and discloses a soil solidification and stabilization agent and application thereof and a blast furnace slag resource utilization method, the solidification and stabilization agent is prepared from blast furnace fly ash, expanded slag, sewage plant sludge and a naphthalene water reducer; the mass ratio of the blast furnace fly ash to the expanded slag to the sewage plant sludge to the naphthalene water reducer is (20-40): (20-30): (40-60): (10-15); the sewage plant sludge is the sewage plant sludge obtained in a Fenton process. According to the soil solidification stabilizer provided by the invention, through the combined action of the blast furnace fly ash, the expanded slag, the sewage plant sludge and the naphthalene water reducer, leaching of high-concentration heavy metals such as lead and cadmium, arsenic and a semi-volatile organic compound benzo (a) pyrene in soil can be effectively inhibited at the same time, so that the underground water IV-class standard is met.