10 results about "Rabbit Antibody" patented technology

The invention discloses a lateral flow biosensor for joint detection of SAA and CRP based on an AIE material and application, the lateral flow biosensor comprises a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, the combination pad is coated with fluorescent nano-microsphere labeled antibodies AIE490NP-SAA, AIE490NP-RIgG and AIE610NP-CRP, and the nitrocellulose membrane is coated with fluorescent nano-microsphere labeled antibodies AIE490NP-RIgG and AIE610NP-CRP. A detection line T line and a quality control line C line are sprayed on the nitrocellulose membrane, the T line is formed by an SAA coating antigen and a CRP coating antigen which are coated on the nitrocellulose membrane, and the C line is formed by a goat anti-rabbit IgG antibody coated on the nitrocellulose membrane. The prepared lateral flow biosensor can simultaneously detect CRP and SAA, has high sensitivity and good repeatability, and has wide application prospects in differential diagnosis of early infectious diseases.

The present application relates to the technical field of antibody detection, in particular to a TGEV IgA antibody fluorescent microsphere immunochromatography test strip and preparation and application thereof.The TGEV IgA antibody fluorescent microsphere immunochromatography test strip comprises a base plate, and a sample pad, a nitrocellulose membrane and a water absorption pad which are sequentially and lapped and pasted on the base plate; the sample pad is a glass fiber membrane, and the glass fiber membrane is coated with a fluorescent microsphere labeled TGEV-S1 recombinant protein conjugate and a fluorescent microsphere labeled rabbit IgG antibody conjugate; the nitrocellulose membrane is provided with a detection line and a quality control line, wherein the detection line is coated with a goat anti-pig IgA antibody, and the quality control line is coated with a goat anti-rabbit IgG antibody.The test strip can accurately capture low-titer TGEV IgA antibodies, is suitable for high-throughput rapid on-site detection, and has the characteristics of low reagent cost and detection difficulty, high sensitivity and high specificity.

The invention relates to the technical field of gene engineering, and particularly discloses a one-step RT-PCR (Reverse Transcription-Polymerase Chain Reaction) kit for amplifying a complete sequence of a variable region of a rabbit-derived antibody and a use method of the one-step RT-PCR kit. The kit comprises an One-Step RT Enzyme Mix, a 5 * One-Step RT Buffer, three pairs of specific amplification primers, a positive control, a negative control and a Nuclease-free Water, and is characterized in that the specific amplification primers are used for amplification of the Nuclease-free Water, and the specific amplification primers are used for amplification of the Nuclease-free Water. The three pairs of primers are designed according to rabbit immune globulin gene sequences and can fully cover heavy chain and light chain variable regions. The use method comprises the following steps: sorting positive B cells from rabbit spleen, cracking single cells, continuously performing reverse transcription and PCR (Polymerase Chain Reaction) amplification in the same reaction system by taking a cracking product as a template, and identifying and cloning the product. The kit can efficiently and specifically obtain the complete sequence of the variable region of the rabbit antibody from the single B cell, and has the advantages of wide coverage, high sensitivity, simplicity and convenience in operation and reliable result.

The invention belongs to the technical field of biological detection, and discloses an allergen-specific IgE antibody detection card, a kit and application. The allergen specificity IgE antibody detection card comprises a combination pad and a nitrocellulose membrane, wherein the combination pad is coated with a mouse anti-human IgE antibody marked by AIE polystyrene fluorescent microspheres and a rabbit IgG antibody marked by AIE polystyrene fluorescent microspheres; a detection line of the nitrocellulose membrane is coated with a specific antigen, and a quality control line of the nitrocellulose membrane is coated with a goat anti-rabbit IgG antibody. The kit prepared from the reagent card is used for detecting the allergen-specific IgE antibody, has the characteristics of high detection sensitivity, high specificity, simplicity in operation, cost saving and the like, and has a very large market and a good application prospect.

Using rabbit antibody technology, an anti-TfR1 monoclonal antibody having high affinity, high specificity and high blood safety is obtained by means of phage immune library construction and screening, and the anti-TfR1 monoclonal antibody is subjected to humanization and affinity maturation treatment to obtain a humanized anti-TfR1 monoclonal antibody having high affinity without inducing immunogenicity.

The present invention discloses a kit and a detection kit for detecting herpes simplex virus in tears, the kit comprises: a housing, which comprises an internal cavity, and a sample application area and a detection window which are respectively communicated with the internal cavity; the immunochromatography test strip comprises a sample pad, a release pad and a nitrocellulose membrane which are connected in sequence, the immunochromatography test strip is arranged in the inner containing cavity, and part or all of the sample pad extends to the sample application area; the sampling assembly is in pivot connection with the shell and limits a first limit position, and when the sampling assembly is located at the first limit position, the sampling assembly is in surface contact with the sample pad; wherein herpes simplex virus type 1 G glycoprotein marked by fluorescence is embedded on the release pad; the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is coated with an anti-human IgA antibody, the quality control line is coated with a goat anti-rabbit IgG antibody, and the detection line and the quality control line are exposed to the detection window.

This invention provides a CA15-3 antigen-binding protein and its application. The CA15-3 antigen-binding protein includes a heavy chain variable region and a light chain variable region. The heavy chain variable region includes CDR3 having the sequence shown in SEQ ID NO. 7 or SEQ ID NO. 13; the light chain variable region includes CDR3 having the sequence shown in SEQ ID NO. 10 or SEQ ID NO. 16. The CA15-3 antigen-binding protein of this invention is a rabbit monoclonal antibody. Compared with mouse antibodies and polyclonal antibodies, rabbit antibodies naturally have higher affinity and stronger specificity. Furthermore, the combined antibody pair exhibits high specificity and accuracy. The obtained CA15-3 antigen-binding protein can be paired for the diagnosis of breast cancer.

This invention belongs to the field of pathogen and toxin detection technology, specifically relating to a polyclonal antibody colloidal gold test strip for detecting Clostridium perfringens α toxin and its application. The polyclonal antibody colloidal gold test strip includes a PVC base plate, on which a sample pad, a gold-labeled pad, a chromatography membrane, and an absorbent pad are sequentially overlapped. The gold-labeled pad is coated with colloidal gold particles labeled with polyclonal antibodies against Clostridium perfringens α toxin. A detection line and a control line are sequentially arranged on the chromatography membrane. The detection line is coated with polyclonal antibodies against Clostridium perfringens α toxin, and the control line is coated with goat anti-rabbit IgG (H+L) antibody. The amino acid sequence of the recombinant protein of Clostridium perfringens α toxin is shown in SEQ ID NO.3. The polyclonal antibody colloidal gold test strip provided by this invention can be used for the detection of Clostridium perfringens α toxin, with a detection limit of 31.25 μg / mL.

This invention provides a monoclonal antibody that is universally specific to human IgG but does not bind to monkey IgG. [Solution] An anti-human IgG antibody obtained from rabbits, its antigen-binding portion, and a method for using the antibody and portion are provided.

An AMH semi-quantitative latex-method detection kit, comprising two test strips having different sensitivities. Each test strip comprises a backing plate (5), a sample pad (4), a conjugate pad (3), a nitrocellulose membrane (2), and an absorbent pad (1). The conjugate pad (3) is sprayed with a latex microsphere conjugate solution, the latex microsphere conjugate comprising latex microsphere-labeled AMH capture antibodies and rabbit IgG antibodies. The nitrocellulose membrane (2) is provided with a test line and a control line, the control line and the test line being respectively sprayed with goat anti-rabbit IgG and AMH monoclonal antibodies. The test strips having different sensitivities achieve rapid semi-quantitative detection of AMH levels in whole blood samples without any equipment or other auxiliary reagents. Analysis can be performed immediately at the sampling site, enabling convenient operation.