23 results about "Rabbit Antibody" patented technology

The invention relates to a system for monitoring mRNA m6A methylation in hepatic stellate cells based on a hybridization chain reaction and a detection method, and belongs to the technical field of analysis and detection. In order to solve the problem of poor sensitivity and detection precision in the prior art, the invention provides a system for monitoring mRNA m6A methylation in hepatic stellate cells based on hybridization chain reaction and a detection method, the method comprises the following steps: contacting a target mRNA m6A methylation sample to be detected with a group of probes comprising a probe a and a probe b; the probe b is connected to an anti-rabbit IgG antibody to form a probe b attached with the anti-rabbit IgG antibody; combining with a rabbit monoclonal anti-m6A antibody; the rabbit monoclonal anti-m6A antibody can be combined with a target mRNA m6A methylated sample to be detected; a hairpin probe H1 monomer and a hairpin probe H2 monomer are subjected to a hybridization chain reaction to form a polymer, and imaging detection is carried out. The method has the advantages of high detection sensitivity, high detection selectivity and strong intuition.

The invention belongs to the technical field of Ultrap1 enzyme quantitative detection, and particularly relates to an Ultrap1 enzyme double-antibody sandwich ELISA (enzyme-linked immuno sorbent assay) kit and application. Comprising an elisa plate coated with an anti-Ultrap1 enzyme mouse monoclonal antibody, an anti-Ultrap1 enzyme rabbit polyclonal antibody, an HRP-labeled goat anti-rabbit antibody, a diluent, a cleaning solution, a confining solution, a developing solution and a stop solution. According to the present invention, the complex of the Ulp1 enzyme and the SUMO tag protein is adopted as the immunogen to simultaneously immunize mice and New Zealand rabbits so as to prepare different species of the good affinity Ulp1 enzyme antibodies, and then the optimal reaction condition is further screened to develop the stable double-antibody sandwich ELISA detection kit with characteristics of high specificity and strong sensitivity, the defects in the prior art can be effectively overcome.

The invention provides a progesterone detection reagent strip based on an antibody synergistic effect and a preparation method of the progesterone detection reagent strip. The progesterone detection reagent strip comprises a bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, the combination pad is sprayed with fluorescent nano-microspheres coupled with a goat-derived PROG antibody and fluorescent nano-microspheres coupled with a goat anti-rabbit antibody, a detection line of the nitrocellulose membrane is coated with a PROG antigen, and a quality control line of the nitrocellulose membrane is coated with a rabbit anti-goat IgG antibody. According to the progesterone detection reagent strip based on the antibody synergistic effect, the competition method detection principle is adopted, the fluorescent microspheres are used as markers, the progesterone antibody and the fluorescent microspheres are combined in a coupling mode, the specificity and binding efficiency of antigen-antibody reaction are optimized based on the antibody synergistic effect, and the progesterone detection reagent strip can be used for detecting progesterone. The stability and sensitivity of the detection system are remarkably improved, and high-sensitivity and high-specificity detection of the progesterone level in serum or plasma is achieved.

The present invention discloses the preparation of antibodies against sickle cell hemoglobin variants and the application of a typing detection kit, belonging to the field of biomedicine. By comparing the sequences of different hemoglobins, the present invention analyzes the key mutation points of sickle cell hemoglobin. A prokaryotic expression system is used to recombinantly express the hemoglobin α chain (HBA) and hemoglobin β chain (including HBB, HBS, and HBC). The target proteins of different typings are then conjugated with KLH to prepare immunogens. Animals are then immunized and screened for antibodies targeting the different hemoglobin variants. Rabbit antibodies are obtained for HBB and HBS proteins, while mouse antibodies are obtained for HBA and HBC. DNA sequencing is used to analyze the antibody sequence information. The present kit has high sensitivity and specificity, and can rapidly distinguish hemoglobin typing, thus overcoming the shortcomings of existing detection technologies and assisting in clinical infection control and treatment.

The invention relates to the technical field of detection reagents, in particular to a test strip for detecting mites, application of the test strip and a kit for detecting the mites. The test strip comprises a sample pad, a combination pad, a nitrocellulose membrane and absorbent paper, the combination pad is coated with an anti-mite antibody marked by colloidal gold; the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is coated with a mite antigen, and the quality control line is coated with a goat anti-rabbit IgG antibody. The test strip is prepared by using a colloidal gold method, and the adopted principle is a double-antibody sandwich method. By utilizing the test strip disclosed by the invention, whether the acarid is contained or not can be obtained within 5-10 minutes without an extra detection instrument, and the detection method is high in accuracy. The possibility is provided for efficiently and quickly detecting the mites.

The invention discloses a lateral flow biosensor for joint detection of SAA and CRP based on an AIE material and application, the lateral flow biosensor comprises a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, the combination pad is coated with fluorescent nano-microsphere labeled antibodies AIE490NP-SAA, AIE490NP-RIgG and AIE610NP-CRP, and the nitrocellulose membrane is coated with fluorescent nano-microsphere labeled antibodies AIE490NP-RIgG and AIE610NP-CRP. A detection line T line and a quality control line C line are sprayed on the nitrocellulose membrane, the T line is formed by an SAA coating antigen and a CRP coating antigen which are coated on the nitrocellulose membrane, and the C line is formed by a goat anti-rabbit IgG antibody coated on the nitrocellulose membrane. The prepared lateral flow biosensor can simultaneously detect CRP and SAA, has high sensitivity and good repeatability, and has wide application prospects in differential diagnosis of early infectious diseases.

The present application relates to the technical field of antibody detection, in particular to a TGEV IgA antibody fluorescent microsphere immunochromatography test strip and preparation and application thereof.The TGEV IgA antibody fluorescent microsphere immunochromatography test strip comprises a base plate, and a sample pad, a nitrocellulose membrane and a water absorption pad which are sequentially and lapped and pasted on the base plate; the sample pad is a glass fiber membrane, and the glass fiber membrane is coated with a fluorescent microsphere labeled TGEV-S1 recombinant protein conjugate and a fluorescent microsphere labeled rabbit IgG antibody conjugate; the nitrocellulose membrane is provided with a detection line and a quality control line, wherein the detection line is coated with a goat anti-pig IgA antibody, and the quality control line is coated with a goat anti-rabbit IgG antibody.The test strip can accurately capture low-titer TGEV IgA antibodies, is suitable for high-throughput rapid on-site detection, and has the characteristics of low reagent cost and detection difficulty, high sensitivity and high specificity.

The invention relates to the technical field of gene engineering, and particularly discloses a one-step RT-PCR (Reverse Transcription-Polymerase Chain Reaction) kit for amplifying a complete sequence of a variable region of a rabbit-derived antibody and a use method of the one-step RT-PCR kit. The kit comprises an One-Step RT Enzyme Mix, a 5 * One-Step RT Buffer, three pairs of specific amplification primers, a positive control, a negative control and a Nuclease-free Water, and is characterized in that the specific amplification primers are used for amplification of the Nuclease-free Water, and the specific amplification primers are used for amplification of the Nuclease-free Water. The three pairs of primers are designed according to rabbit immune globulin gene sequences and can fully cover heavy chain and light chain variable regions. The use method comprises the following steps: sorting positive B cells from rabbit spleen, cracking single cells, continuously performing reverse transcription and PCR (Polymerase Chain Reaction) amplification in the same reaction system by taking a cracking product as a template, and identifying and cloning the product. The kit can efficiently and specifically obtain the complete sequence of the variable region of the rabbit antibody from the single B cell, and has the advantages of wide coverage, high sensitivity, simplicity and convenience in operation and reliable result.

The invention discloses preparation of an anti-sickle cell anemia hemoglobin variant antibody and application of a typing detection kit, and belongs to the field of biological medicine. Through sequence comparison of different hemoglobin, key mutation point structures of hemoglobin of sickle anemia are analyzed, a prokaryotic expression system is adopted for recombinant expression of hemoglobin alpha chains (HBA) and hemoglobin beta chains (containing HBB, HBS and HBC), and target proteins of different types are coupled with KLH to prepare immunogens. Respectively carrying out animal immunization and screening corresponding antibodies aiming at different mutants of hemoglobin. Wherein the HBB protein and the HBS protein obtain rabbit source antibodies, and the HBA protein and the HBC protein obtain mouse source antibodies; according to the present invention, the kit has advantages of high sensitivity, good specificity, rapid detection capable of distinguishing hemoglobin typing, compensation of the defects of the existing detection technology, and clinical infection control and treatment assistance.

The invention belongs to the technical field of biological detection, and discloses an allergen-specific IgE antibody detection card, a kit and application. The allergen specificity IgE antibody detection card comprises a combination pad and a nitrocellulose membrane, wherein the combination pad is coated with a mouse anti-human IgE antibody marked by AIE polystyrene fluorescent microspheres and a rabbit IgG antibody marked by AIE polystyrene fluorescent microspheres; a detection line of the nitrocellulose membrane is coated with a specific antigen, and a quality control line of the nitrocellulose membrane is coated with a goat anti-rabbit IgG antibody. The kit prepared from the reagent card is used for detecting the allergen-specific IgE antibody, has the characteristics of high detection sensitivity, high specificity, simplicity in operation, cost saving and the like, and has a very large market and a good application prospect.

Using rabbit antibody technology, an anti-TfR1 monoclonal antibody having high affinity, high specificity and high blood safety is obtained by means of phage immune library construction and screening, and the anti-TfR1 monoclonal antibody is subjected to humanization and affinity maturation treatment to obtain a humanized anti-TfR1 monoclonal antibody having high affinity without inducing immunogenicity.

The invention relates to the technical field of biological medicine, in particular to a polyclonal antibody aiming at near-infrared fluorescent protein emiRFP as well as preparation and application of the polyclonal antibody. The preparation method comprises the following steps: taking the emiRFP polypeptide as shown in SEQ ID NO.1 as an antigen immune animal, collecting serum, and collecting the polyclonal antibody aiming at the emiRFP from the serum. Compared with the prior art, the polyclonal rabbit antibody has the beneficial effects that the polyclonal rabbit antibody of the near-infrared fluorescent protein emiRFP is successfully prepared for the first time, the technical blank is filled, and an effective supplement scheme is provided for detection and application of the near-infrared fluorescent protein.

Herein is reported an antibody binding to rabbit CD19 comprising (a) a HVR-H1 comprising the amino acid sequence of SEQ ID NO: 32 or 33 or 34, (b) a HVR-H2 comprising the amino acid sequence of SEQ ID NO: 35 or 36, (c) a HVR-H3 comprising the amino acid sequence of SEQ ID NO: 37, (d) a HVR-L1 comprising the amino acid sequence of SEQ ID NO: 38, (e) a HVR-L2 comprising the amino acid sequence of SEQ ID NO: 39, and (f) a HVR-L3 comprising the amino acid sequence of SEQ ID NO: 40, as well as methods of using the same, especially in the identification and selection of antibody producing rabbit B-cells.

The present invention discloses a kit and a detection kit for detecting herpes simplex virus in tears, the kit comprises: a housing, which comprises an internal cavity, and a sample application area and a detection window which are respectively communicated with the internal cavity; the immunochromatography test strip comprises a sample pad, a release pad and a nitrocellulose membrane which are connected in sequence, the immunochromatography test strip is arranged in the inner containing cavity, and part or all of the sample pad extends to the sample application area; the sampling assembly is in pivot connection with the shell and limits a first limit position, and when the sampling assembly is located at the first limit position, the sampling assembly is in surface contact with the sample pad; wherein herpes simplex virus type 1 G glycoprotein marked by fluorescence is embedded on the release pad; the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is coated with an anti-human IgA antibody, the quality control line is coated with a goat anti-rabbit IgG antibody, and the detection line and the quality control line are exposed to the detection window.

This invention provides a CA15-3 antigen-binding protein and its application. The CA15-3 antigen-binding protein includes a heavy chain variable region and a light chain variable region. The heavy chain variable region includes CDR3 having the sequence shown in SEQ ID NO. 7 or SEQ ID NO. 13; the light chain variable region includes CDR3 having the sequence shown in SEQ ID NO. 10 or SEQ ID NO. 16. The CA15-3 antigen-binding protein of this invention is a rabbit monoclonal antibody. Compared with mouse antibodies and polyclonal antibodies, rabbit antibodies naturally have higher affinity and stronger specificity. Furthermore, the combined antibody pair exhibits high specificity and accuracy. The obtained CA15-3 antigen-binding protein can be paired for the diagnosis of breast cancer.

The invention discloses a circulating tumor cell bimodal co-localization model and application thereof. The bimodal co-localization model comprises a morphological analysis unit and an immunofluorescence signal analysis unit for circulating tumor cells, wherein the morphological analysis unit comprises Diff staining, and the immunofluorescence signal analysis unit comprises a CD45 mouse antibody, a 488-mouse secondary antibody, a CK rabbit antibody and a 594-rabbit secondary antibody. The circulating tumor cell bimodal co-localization model has the advantages that in the application process of the circulating tumor cell bimodal co-localization model, a non-antibody-dependent nano magnetic bead enrichment technology is adopted, so that the completeness and cell viability of CTC are improved, and the success rate of subsequent multi-step dyeing is ensured; the use amount of the sample and the collection frequency are reduced; the use amount of the peripheral blood sample is reduced through double analysis (morphology and molecular markers), so that the clinical universality is improved; in combination with morphology and immunofluorescence detection, the risk of false positive and false negative is reduced, and the diagnosis accuracy of CTC is improved.

This invention belongs to the field of pathogen and toxin detection technology, specifically relating to a polyclonal antibody colloidal gold test strip for detecting Clostridium perfringens α toxin and its application. The polyclonal antibody colloidal gold test strip includes a PVC base plate, on which a sample pad, a gold-labeled pad, a chromatography membrane, and an absorbent pad are sequentially overlapped. The gold-labeled pad is coated with colloidal gold particles labeled with polyclonal antibodies against Clostridium perfringens α toxin. A detection line and a control line are sequentially arranged on the chromatography membrane. The detection line is coated with polyclonal antibodies against Clostridium perfringens α toxin, and the control line is coated with goat anti-rabbit IgG (H+L) antibody. The amino acid sequence of the recombinant protein of Clostridium perfringens α toxin is shown in SEQ ID NO.3. The polyclonal antibody colloidal gold test strip provided by this invention can be used for the detection of Clostridium perfringens α toxin, with a detection limit of 31.25 μg / mL.

This invention provides a monoclonal antibody that is universally specific to human IgG but does not bind to monkey IgG. [Solution] An anti-human IgG antibody obtained from rabbits, its antigen-binding portion, and a method for using the antibody and portion are provided.

An AMH semi-quantitative latex-method detection kit, comprising two test strips having different sensitivities. Each test strip comprises a backing plate (5), a sample pad (4), a conjugate pad (3), a nitrocellulose membrane (2), and an absorbent pad (1). The conjugate pad (3) is sprayed with a latex microsphere conjugate solution, the latex microsphere conjugate comprising latex microsphere-labeled AMH capture antibodies and rabbit IgG antibodies. The nitrocellulose membrane (2) is provided with a test line and a control line, the control line and the test line being respectively sprayed with goat anti-rabbit IgG and AMH monoclonal antibodies. The test strips having different sensitivities achieve rapid semi-quantitative detection of AMH levels in whole blood samples without any equipment or other auxiliary reagents. Analysis can be performed immediately at the sampling site, enabling convenient operation.

The invention belongs to the technical field of immunoassay, and particularly relates to a multicolor joint detection test strip for detecting transgenosis, which comprises a bottom plate, and a sample pad, a combination pad, an NC membrane and a water absorption pad which are fixed on the bottom plate and are connected in sequence, the conjugate pad is coated with a first microballoon-CP4mAb1 compound and a second microballoon-BTmAb1 compound; a detection line T1 and a detection line T2 are marked on the NC membrane by using CP4mAb2 and BTmAb2 respectively, and a quality control line is marked by using a goat anti-rabbit IgG antibody; wherein the first microballe-CP4mAb1 compound is a compound formed by coupling a first nano microsphere and a first antibody of a CP4EPSPS protein, and the second microballe-BTmAb1 compound is a compound formed by coupling a second nano microsphere and a first antibody of a BT-Cry1Ab / Ac protein; the CP4mAb2 is a second antibody of a CP4EPSPS protein, and the BTmAb2 is a second antibody of a BT-Cry1Ab / Ac protein. The test strip provided by the invention can be used for simultaneously detecting the BT-Cry1Ab protein, the BT-Cry1Ac protein and the CP4EPSPS protein, the detection result is accurate, the specificity and the stability are good, the sensitivity is relatively high, the screening efficiency can be remarkably improved, and the cross interference among the components is effectively reduced.

The application provides an immunohistochemical double-staining three-marker kit for breast invasive carcinoma Ki-67 index interpretation, which comprises a mixed primary antibody; the mixed primary antibody comprises a mouse antibody and a rabbit antibody; the mixed primary antibody comprises a myoepithelial marker antibody, a glandular epithelial marker antibody and a Ki-67 antibody; the myoepithelial marker antibody and the glandular epithelial marker antibody are located in the cytoplasm and / or cell membrane. Different from the prior art, the above technical scheme adopts the mixed primary antibody of the myoepithelial marker antibody, the glandular epithelial marker antibody and the Ki-67 antibody, can simultaneously and synchronously mark on the same breast or breast cancer tissue section, and accurately and quickly distinguishes the invasive carcinoma and the carcinoma in situ and the interstitial and lymphocyte regions through the immunohistochemical multiplex staining mode. Combined with morphological characteristics, through the location and color difference, the invasive carcinoma region is obviously displayed, and the percentage of Ki-67 positive cells in the invasive carcinoma region is calculated.

This invention discloses a method and application for detecting goblet cell-related antigen channels in the piglet intestine. It relates to the field of antigen detection technology. The method includes the following steps: taking a portion of the mid-jejunum from a piglet; processing it; cutting the processed mid-jejunum into small pieces; incubating the pieces in a culture medium containing a marker; fixing; dehydrating and clearing the fixed pieces; embedding them in paraffin; preparing tissue sections; staining the tissue sections with a rabbit antibody against the goblet cell marker protein mucin 2 and a goat anti-rabbit fluorescent secondary antibody; mounting with the nuclear dye DAPI; and observing and analyzing under a fluorescence microscope. The method provided by this invention is simple to operate and can successfully detect goblet cell-related antigen channels in weaned piglets, laying the foundation for subsequent pig nutrition formulations.

The present invention discloses a test strip for detecting neutralizing antibodies to human adenovirus type 5. The test strip comprises a PVC base plate, and a sample pad, a fluorescent pad, a chromatographic membrane, and an absorbent pad arranged in sequence above the PVC base plate. The chromatographic membrane comprises a solid-phase nitrocellulose membrane consisting of a test line and a quality control line. The test line is coated with mouse anti-human IgG antibodies, the quality control line is coated with goat anti-rabbit IgG antibodies, and the fluorescent pad is coated with quantum dot-labeled rabbit IgG and quantum dot-labeled antigens. The quantum dot-labeled antigens are either or both of quantum dot-labeled human adenovirus type 5 fiber knob protein and quantum dot-labeled human adenovirus type 5 intact inactivated virus. The present invention also discloses a method for preparing the test strip, as well as a test card and kit using the same. The present invention can be used to rapidly assess the level of neutralizing antibodies to adenovirus type 5 in a population, providing guidance for adenovirus vector therapy methods, and the like.