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Benzopyrene rapid detection card

A detection card, benzopyrene technology, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., achieves the effects of easy preparation, high sensitivity, and saving detection costs

Inactive Publication Date: 2011-01-26
无锡安迪生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The third is the vegetable oil refining process, repeated high-temperature heating, baking, steaming and frying tea cakes are prone to produce benzopyrene

Method used

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  • Benzopyrene rapid detection card
  • Benzopyrene rapid detection card

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A kind of benzopyrene quick detection card, it is to arrange test strip 2 in detection card shell 1, the structure of test card shell 1 and the setting of test strip 2 refer to figure 1 , The detection card shell 1 is a long flat thin shell shell, 70mm long, 20mm wide, 5mm thick, and the thin shell wall thickness is 1mm, made of engineering plastics. The detection card casing 1 is composed of two halves of a casing cover and a casing seat, and a detection window 3 and a sample injection hole 4 are arranged on the casing cover of the detection card casing 1 . The test strip 2 is placed in the shell seat of the test card shell 1 .

[0025] The test strip 2 is a narrow sheet with a multilayer structure, the length of the sheet is about 60mm, the width of the sheet is about 4mm, and the thickness is about 2.5mm. The test strip 2 has a multi-layer structure: the bottom layer is a supporting backboard 5, which is a polyvinyl chloride plastic sheet with a thickness of about 0...

Embodiment 2

[0028] The sample processing method is:

[0029] The treatment method for the edible oil sample is: weigh 1mL edible oil sample, add 1mL sample extract (pH 7.6, mixed solution containing 50% acetone and 10mmol PBST by volume fraction), stir evenly in the test tube, and then use sample diluent (pH7.6, solution containing 10mmol PBST) diluted to 2 times volume for detection;

[0030] The treatment method for fried food samples is: take 1 g of mashed fried food samples, add 2 mL of sample extract (pH 7.6, a mixed solution containing 50% acetone and 10 mmol PBST by volume fraction), stir evenly in a test tube, Then use the sample diluent (pH7.6, solution containing 10mmol PBST) to dilute to 2 times the volume for detection;

[0031] When using the benzopyrene rapid detection card for detection, the sample liquid to be detected is dripped from the sample hole 4 into the sample pad 8 of the benzopyrene rapid detection card test strip 2, and due to the principle of siphon action, th...

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Abstract

A benzopyrene rapid detection card belongs to the technical field of food detection. A test strip is arranged in the casing of the benzopyrene rapid detection card; the test strip is formed by sticking a sample pad, a colloidal gold film, a nitrocellulose film and a water absorbent film on a test strip support back plate in turn, wherein the colloidal gold film is a fiberglass membrane containing the anti-benzopyrene antibody colloidal gold marker; and the nitrocellulose film is provided with two display belts, one is a detection belt containing benzopyrene-protein conjugate, the other is a quality control containing anti-rabbit antibody or anti-rat antibody. The invention has the advantage that the immunological method is used to directly detect the content of benzopyrene in samples such as edible oil and fried food. The benzopyrene rapid detection card is easy to prepare and convenient and fast to use, and the detection result is accurate.

Description

technical field [0001] The invention relates to a quick detection card for benzopyrene, which is a detection device for detecting benzopyrene and belongs to the technical field of food detection. Background technique [0002] Benzopyrene (3,4-Benzopyrene, benzo[a]pyrene), also known as 3,4-benzopyrene, is a polycyclic aromatic hydrocarbon composed of five benzene rings. It was first isolated from asphalt in 1933. carcinogenic hydrocarbons. It is a light yellow crystalline solid at room temperature, with a melting point of 179°C and a boiling point of 312°C. It is hardly soluble in water, but easily soluble in organic solvents such as benzene, toluene, acetone, ethane, etc. It is stable under alkaline conditions and easily reacts with acids. Changes, properties are relatively stable, the chemical half-life in the atmosphere is less than 1 day when there is sunlight, and several days when there is no sunlight. The half-life of 3,4-benzopyrene in the surface layer of the wate...

Claims

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Application Information

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IPC IPC(8): G01N33/558
Inventor 周坚
Owner 无锡安迪生物工程有限公司
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